Rorug06G0096900

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006431
Physical Location & Seq
Reverse (-)
12940747 .. 12970089
29343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0096900.1

Sequence Viewer

Length: 2883 bp
ATGGCAGCACAAACCGAGCTTAACATCACCACAGACCGGTCTGCTCTTCTTGCTCTCAATGCCCGTATCACCAGTGACCCTCAAAACATGATCTTCACCAACTGGTCAACCACTACTCCTATTTGCAACTGGGTTGGGGTCACTTGTGGTGCACGCCATCATCGAGTCGCAGACTTGAACCTCTCTTACTTTGGTCTCACAGGCACCATTCCTCCGGAGTTAGGAAACTTATCATTTCTTGTTAATATGGACTTCAGCAATAACAATTTCCATGGTATATTGCCTCAGGAATTGGCTCGCCTGCGTCGGTTGAAGTTTATCAGCTTGAGAAAAAACAAGTTCATGAGAGTCATTCCGTCATGGTTTGGGTCCTTATTCAAACTTCAAGTATTCAGTTTGTTTGGTAATCAATTTTCAGGTTCCATACCTGCTACAATCTTCAACTTATCTGCTCTGCAAAAAATAGATCTGAGGGATAACCAACTCTCAGGCACCATTCCTCCGCAACTAGGCAACTTATCTTTTCTTGTTGATCTAGACTTGAGAAATAATAGTTTTCACGGTCCTATACCATATGAGATTGGTTATCTTCCAAATTTAGAGGAATTGTCACTCGGTGGTAATAATCTACATGGCCATATCCCATCCTCAATCTTCAATATCTCCACAATAAAAGTATTATATCTCAACTTCAATAAGCTCTCAGGTAGCCTCCCTGCAAACATAGGCCTTGGGCTTCCAAACCTAGAAGTGTTTACTGCAGGATCGAATCACCTCAGTGGAGTAATCCCCGAATCCATCTCCAATGCTTCTAAGCTTCGGATTCTAGACCTGGGAGGTAACTCTTTTTCTGGATTTATTCCAACCACTCTGTGCCTCTTGACAAACCTTGAATTCCTCAGATTGGTCTTCAATAATTTGACCATTGATGCCTCTACTCCAGAAGTTAATATTTTTTCTTGTCTGCCAAATCTTAGAAATTTAAGGATTTTAGCCTTTGAAAGAAATCCATTTGATGCCAAGCTTTCAATTTCCTTCGGCAATTTCTCTGCATCACTTCAATATCTTTATTTAGGAAATTGCAGATTCAGAGGTAACATCCCCAGTGATATTGGGAATTTGAGTAGCTTGATATTGTTATCCCTCGATGAGAATCAATTGAGCGGGTCAATTCCAAGCTCAATGGGAAGACTACTGAATCTTCAAGGTTTGTATATGTATGATAACGCATTGCAAGGATACATTCCAGATGAACTTTGTCAACTAGAAGACTTAGATGTTTTGTATTTGGGTGGGAATCAACTCTCTGGTTTTGTACCTTCCTGCTTGGGTAATCTAACAACATCTCTTAGATATCTATCCTTAGATTCCAATTTGTTGAATTCCACAATACCATCTACCTTGTGGGGACTTACAGATATCTTGGAACTAGACTTGTCATCCAATTCTCTAATTGGGCCTCTATCAGCTGCTGTTGGTAATTTGAAAGTTGCTACATACATAAATGTATCATACAACCAATTATCTGGGAGTGTACCAAGTAGCATTGGGAGTTTAGTGAGTTTGGTCAATCTCTCCTTAGCACATAACAATCTAGAAGGCCCTATTCCTAGTTCCTTTGAAGATTGTGTAAGCTTAGAAGGATTGGATTTATCTCGAAATAGTTTCACTGGAGTGATTCCAAAGTCTCTAGAAGCACTCTCATATCTCAAGCATCTGAATTTGTCTTTCAACAGACTCCAAGGAGAAATTCCAAGCGGCGGTCCTTTCAAAAACTTCTCTGCTGAATCATTTGTTTCAAACCATGGACTCTGTGGTGGATCCCAATTTCAAGTTCCACCATGCAAAAGAAAAACTAGCATATCTATCTTGAAGTATGTTATTCCGGGGATCTTGTCAGCAATGCTTCTAGCGGCCTCCATATTTTGGATGTTGATGTTACACAGAAAAAACAATGCAATAGCTGCAGCACATACTATCTTGACACCTCAAGTTTTCTGGAGAAGAGTTTCATACATAGAGCTCCTACATGCGACAGATGGATTCAATGAAAGCAACTTACTTGGTATTGGGGGTTTTGGCTCAGTATATAAAGGAACATTGTCAGATGGGATAGACGTTGCGATAAAGGTTTTCAATTTGGAGTTAGAAGGGGCATTTACAAGTTTTGAGGTTGAATGTGAAGTGCTAAGTAATGTTCGTCACCGAAATCTTGTCAAAGTCATCAGTTGCTGCAGTCAAATTGATTTCAAAGGTCTTGTATTGAATTACATGCCTAATGGGAGCCTTGACAAGTGGTTGTATTCTCAGAATTCTTCTTTGAACATAATGCAGAGGTTGGATATAATGATAGATGTTGCATCGGCATTAGAGTACCTTCATCATGGTTATGAAGTACCTATTGTCCATTGTGATTTGAAGCCAAGCAACAACCTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGTATTGCGAGACTCTTGGGTGGAGGAGATTCTATGACTCAAACCATGACTCTAGCCACAATTGGGTATACTGCTCCAGTGATGGAAACATTCACAAGAAGGAAGCCAACCGATGAGATCTTCATTGGGGACATGAGATTTAATCAATGGGTTGCAAGTTTTCTAGATGCGGATGCGATAGCTGAAGTTGTGGATGCTACTTTACTTGGAACCGAGGAGGATCATGAGTTCGTGAGCAAGAGGGAGTGCTTATCATCTATTATGAGATTAGCTGTTGCTTGTTCAGCAGAATCTCCGGAAGAGAGGATAAACATGCAAGAGGCTGTAGCCATGCTTAAAACAATCAAGATCAAGTTTTCAAAGCAAACTGCAGCAGGAGGTATGGTGTTGAACTGTCGTCCTGTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

960

Amino Acids

105.25

Weight (kDa)

5.67

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 11 - 49 2.3e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 60 - 134 8.5e-06 Leucine-rich repeat region
LRR_8 PF13855 102 - 162 2.7e-06 Leucine rich repeat
LRR_14 PF23598 123 - 235 1.9e-06 Leucine-rich repeat region
LRR_8 PF13855 247 - 307 1.3e-06 Leucine rich repeat
LRR_14 PF23598 312 - 458 5.3e-11 Leucine-rich repeat region
LRR_8 PF13855 537 - 580 3e-07 Leucine rich repeat
Pkinase PF00069 683 - 850 5.9e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 686 - 934 3.2e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 2 cut(s) 203, 491
AccB7I CCANNNNNTGG 1 cut(s) 1926
AccBSI CCGCTC 1 cut(s) 1164
AccI GTMKAC 1 cut(s) 2540
AccIII TCCGGA 2 cut(s) 214, 2767
AciI CCGC 6 cut(s) 503, 1164, 1758, 1761, 1913, 2642
AclWI GGATC 5 cut(s) 772, 1815, 1828, 1898, 2700
AcoI YGGCCR 1 cut(s) 634
AcsI RAATTY 8 cut(s) 595, 893, 979, 1117, 1381, 1720, 1749, 2311
AcuI CTGAAG 2 cut(s) 238, 2676
AdeI CACNNNGTG 2 cut(s) 617, 873
AfaI GTAC 4 cut(s) 1317, 1536, 2375, 2397
AfiI CCNNNNNNNGG 7 cut(s) 36, 221, 509, 904, 1760, 1926, 2535
AflIII ACRYGT 1 cut(s) 2458
AgeI ACCGGT 1 cut(s) 36
AjnI CCWGG 1 cut(s) 831
AjuI GAANNNNNNNTTGG 4 cut(s) 1734, 1766, 1818, 1850
AleI CACNNNNGTG 2 cut(s) 777, 1673
AloI GAACNNNNNNTCC 2 cut(s) 2684, 2716
Alw21I GWGCWC 2 cut(s) 154, 2025
Alw26I GTCTC 3 cut(s) 200, 1692, 2476
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 5 cut(s) 772, 1815, 1828, 1898, 2700
AlwNI CAGNNNCTG 2 cut(s) 1472, 2232
Aor13HI TCCGGA 2 cut(s) 214, 2767
AoxI GGCC 5 cut(s) 634, 727, 1457, 1600, 1914
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 6 cut(s) 5, 1469, 1964, 1967, 2232, 2843
ApoI RAATTY 8 cut(s) 595, 893, 979, 1117, 1381, 1720, 1749, 2311
AsiGI ACCGGT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 2008
AspS9I GGNCC 5 cut(s) 369, 563, 1457, 1601, 1763
AsuC2I CCSGG 1 cut(s) 1887
AsuHPI GGTGA 5 cut(s) 19, 61, 88, 764, 2195
AvaII GGWCC 3 cut(s) 369, 563, 1763
AxyI CCTNAGG 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 636
BamHI GGATCC 1 cut(s) 1820
BanI GGYRCC 2 cut(s) 203, 491
BanII GRGCYC 1 cut(s) 2025
BbsI GAAGAC 3 cut(s) 901, 1195, 1275
Bbv12I GWGCWC 2 cut(s) 154, 2025
BbvI GCAGC 6 cut(s) 17, 1456, 1951, 1979, 2219, 2855
BccI CCATC 7 cut(s) 165, 652, 806, 1402, 2033, 2102, 2548
BciT130I CCWGG 1 cut(s) 833
BciVI GTATCC 1 cut(s) 1232
BcnI CCSGG 1 cut(s) 1887
BcoDI GTCTC 3 cut(s) 200, 1692, 2476
BfmI CTRYAG 5 cut(s) 759, 1965, 2233, 2796, 2841
BfuAI ACCTGC 1 cut(s) 436
BfuI GTATCC 1 cut(s) 1232
BglII AGATCT 2 cut(s) 466, 2589
BisI GCNGC 8 cut(s) 6, 1470, 1759, 1914, 1965, 1968, 2233, 2844
BlsI GCNGC 8 cut(s) 7, 1471, 1760, 1915, 1966, 1969, 2234, 2845
Bme1390I CCNGG 2 cut(s) 833, 1887
Bme18I GGWCC 3 cut(s) 369, 563, 1763
BmgT120I GGNCC 5 cut(s) 369, 563, 1457, 1601, 1763
BmiI GGNNCC 7 cut(s) 205, 370, 421, 493, 1822, 2285, 2683
BmrFI CCNGG 2 cut(s) 833, 1887
BmrI ACTGGG 2 cut(s) 139, 1098
BmsI GCATC 8 cut(s) 919, 1006, 1061, 1723, 2369, 2629, 2635, 2656
BmuI ACTGGG 2 cut(s) 139, 1098
BpiI GAAGAC 3 cut(s) 901, 1195, 1275
BpmI CTGGAG 4 cut(s) 924, 1692, 2020, 2532
Bpu10I CCTNAGC 1 cut(s) 1579
BpuEI CTTGAG 4 cut(s) 346, 562, 1694, 1974
BpuMI CCSGG 1 cut(s) 1887
BsaBI GATNNNNATC 2 cut(s) 1152, 1357
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 7 cut(s) 271, 730, 832, 1741, 1804, 1886, 2685
BsaWI WCCGGW 3 cut(s) 36, 214, 2767
BsaXI ACNNNNNCTCC 8 cut(s) 100, 130, 196, 226, 484, 514, 2841, 2871
Bsc4I CCNNNNNNNGG 7 cut(s) 36, 221, 509, 904, 1760, 1926, 2535
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 6 cut(s) 72, 107, 134, 1104, 1675, 2549
Bse21I CCTNAGG 1 cut(s) 285
Bse3DI GCAATG 2 cut(s) 1229, 1908
Bse8I GATNNNNATC 2 cut(s) 1152, 1357
BseAI TCCGGA 2 cut(s) 214, 2767
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 7 cut(s) 271, 730, 832, 1741, 1804, 1886, 2685
BseGI GGATG 6 cut(s) 644, 1098, 1439, 1935, 2650, 2671
BseJI GATNNNNATC 2 cut(s) 1152, 1357
BseLI CCNNNNNNNGG 7 cut(s) 36, 221, 509, 904, 1760, 1926, 2535
BseMI GCAATG 2 cut(s) 1229, 1908
BseMII CTCAG 8 cut(s) 299, 461, 501, 717, 790, 913, 2097, 2321
BseNI ACTGG 6 cut(s) 72, 107, 134, 1104, 1675, 2549
BseRI GAGGAG 2 cut(s) 2511, 2702
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 6 cut(s) 17, 1456, 1951, 1979, 2219, 2855
BshFI GGCC 5 cut(s) 636, 729, 1459, 1602, 1916
BshNI GGYRCC 2 cut(s) 203, 491
BshTI ACCGGT 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 154, 2025
BsiSI CCGG 4 cut(s) 37, 215, 1886, 2768
BslFI GGGAC 2 cut(s) 1422, 2615
BslI CCNNNNNNNGG 7 cut(s) 36, 221, 509, 904, 1760, 1926, 2535
BsmAI GTCTC 3 cut(s) 200, 1692, 2476
BsmFI GGGAC 2 cut(s) 1422, 2615
BsnI GGCC 5 cut(s) 636, 729, 1459, 1602, 1916
Bso31I GGTCTC 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 154, 2025
Bsp13I TCCGGA 2 cut(s) 214, 2767
Bsp143I GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
Bsp19I CCATGG 2 cut(s) 271, 1804
BspACI CCGC 6 cut(s) 503, 1164, 1758, 1761, 1913, 2642
BspANI GGCC 5 cut(s) 636, 729, 1459, 1602, 1916
BspCNI CTCAG 8 cut(s) 298, 462, 500, 716, 789, 912, 2096, 2320
BspEI TCCGGA 2 cut(s) 214, 2767
BspHI TCATGA 2 cut(s) 342, 2695
BspLI GGNNCC 7 cut(s) 205, 370, 421, 493, 1822, 2285, 2683
BspMAI CTGCAG 4 cut(s) 763, 1969, 2237, 2845
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 5 cut(s) 772, 1815, 1828, 1898, 2700
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 2 cut(s) 203, 491
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 1164
BsrDI GCAATG 2 cut(s) 1229, 1908
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 6 cut(s) 72, 107, 134, 1104, 1675, 2549
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 7 cut(s) 271, 730, 832, 1741, 1804, 1886, 2685
BssMI GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
BssNAI GTATAC 1 cut(s) 2541
BssT1I CCWWGG 4 cut(s) 271, 730, 1741, 1804
Bst1107I GTATAC 1 cut(s) 2541
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 2 cut(s) 563, 2867
Bst6I CTCTTC 3 cut(s) 51, 1999, 2766
BstAPI GCANNNNNTGC 2 cut(s) 1964, 2462
BstC8I GCNNGC 3 cut(s) 154, 298, 302
BstDSI CCRYGG 2 cut(s) 271, 1804
BstF5I GGATG 6 cut(s) 644, 1098, 1439, 1935, 2650, 2671
BstKTI GATC 9 cut(s) 93, 469, 535, 767, 1823, 1893, 2592, 2695, 2823
BstMAI GTCTC 3 cut(s) 200, 1692, 2476
BstMBI GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
BstMWI GCNNNNNNNGC 5 cut(s) 50, 59, 1964, 2462, 2756
BstNI CCWGG 1 cut(s) 833
BstNSI RCATGY 4 cut(s) 2033, 2275, 2462, 2788
BstSCI CCNGG 2 cut(s) 831, 1885
BstSFI CTRYAG 5 cut(s) 759, 1965, 2233, 2796, 2841
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 6 cut(s) 17, 1456, 1951, 1979, 2219, 2855
BstV2I GAAGAC 3 cut(s) 901, 1195, 1275
BstX2I RGATCY 4 cut(s) 466, 1820, 1890, 2589
BstXI CCANNNNNNTGG 1 cut(s) 1526
BstYI RGATCY 4 cut(s) 466, 1820, 1890, 2589
BstZ17I GTATAC 1 cut(s) 2541
Bsu36I CCTNAGG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 1232
BsuRI GGCC 5 cut(s) 636, 729, 1459, 1602, 1916
BtgI CCRYGG 2 cut(s) 271, 1804
BtsCI GGATG 6 cut(s) 644, 1098, 1439, 1935, 2650, 2671
BtsIMutI CAGTG 5 cut(s) 79, 784, 1111, 1668, 2556
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 154, 298, 302
CaiI CAGNNNCTG 2 cut(s) 1472, 2232
CciI TCATGA 2 cut(s) 342, 2695
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 5 cut(s) 369, 563, 1457, 1601, 1763
CseI GACGC 1 cut(s) 293
Csp6I GTAC 4 cut(s) 1316, 1535, 2374, 2396
CspAI ACCGGT 1 cut(s) 36
CviQI GTAC 4 cut(s) 1316, 1535, 2374, 2396
DpnI GATC 9 cut(s) 92, 468, 534, 766, 1822, 1892, 2591, 2694, 2822
DpnII GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
DraIII CACNNNGTG 2 cut(s) 617, 873
EaeI YGGCCR 1 cut(s) 634
Eam1104I CTCTTC 3 cut(s) 51, 1999, 2766
EarI CTCTTC 3 cut(s) 51, 1999, 2766
Ecl136II GAGCTC 1 cut(s) 2023
Eco130I CCWWGG 4 cut(s) 271, 730, 1741, 1804
Eco147I AGGCCT 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 2025
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 2 cut(s) 1355, 1420
Eco47I GGWCC 3 cut(s) 369, 563, 1763
Eco53kI GAGCTC 1 cut(s) 2023
Eco57I CTGAAG 2 cut(s) 238, 2676
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 2023
EcoO109I RGGNCCY 2 cut(s) 369, 1601
EcoRI GAATTC 3 cut(s) 893, 1381, 2311
EcoRII CCWGG 1 cut(s) 831
EcoRV GATATC 2 cut(s) 1355, 1420
EcoT14I CCWWGG 4 cut(s) 271, 730, 1741, 1804
EcoT38I GRGCYC 1 cut(s) 2025
ErhI CCWWGG 4 cut(s) 271, 730, 1741, 1804
FaqI GGGAC 2 cut(s) 1422, 2615
FauI CCCGC 1 cut(s) 1157
FauNDI CATATG 1 cut(s) 574
FblI GTMKAC 1 cut(s) 2540
Fnu4HI GCNGC 8 cut(s) 6, 1470, 1759, 1914, 1965, 1968, 2233, 2844
FokI GGATG 6 cut(s) 631, 1085, 1426, 1942, 2657, 2678
FriOI GRGCYC 1 cut(s) 2025
Fsp4HI GCNGC 8 cut(s) 6, 1470, 1759, 1914, 1965, 1968, 2233, 2844
GluI GCNGC 8 cut(s) 6, 1470, 1759, 1914, 1965, 1968, 2233, 2844
GsuI CTGGAG 4 cut(s) 924, 1692, 2020, 2532
HaeIII GGCC 5 cut(s) 636, 729, 1459, 1602, 1916
HapII CCGG 4 cut(s) 37, 215, 1886, 2768
HgaI GACGC 1 cut(s) 293
HincII GTYRAC 2 cut(s) 108, 1262
HindII GTYRAC 2 cut(s) 108, 1262
HindIII AAGCTT 3 cut(s) 815, 1022, 1633
HpaII CCGG 4 cut(s) 37, 215, 1886, 2768
HphI GGTGA 5 cut(s) 19, 61, 88, 764, 2195
Hpy166II GTNNAC 6 cut(s) 108, 152, 756, 1262, 1535, 2541
Hpy188I TCNGA 7 cut(s) 471, 822, 902, 1091, 1719, 2107, 2310
Hpy8I GTNNAC 6 cut(s) 108, 152, 756, 1262, 1535, 2541
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 7 cut(s) 1045, 1329, 1592, 1634, 2144, 2387, 2565
HpyCH4III ACNGT 2 cut(s) 563, 2867
HpyCH4IV ACGT 1 cut(s) 2118
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 59, 1964, 2462, 2756
HpySE526I ACGT 1 cut(s) 2118
Kpn2I TCCGGA 2 cut(s) 214, 2767
Kzo9I GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 3 cut(s) 2028, 2283, 2551
Lsp1109I GCAGC 6 cut(s) 17, 1456, 1951, 1979, 2219, 2855
LweI GCATC 8 cut(s) 919, 1006, 1061, 1723, 2369, 2629, 2635, 2656
MaeII ACGT 1 cut(s) 2118
MaeIII GTNAC 7 cut(s) 74, 139, 609, 839, 1094, 1938, 2201
MalI GATC 9 cut(s) 92, 468, 534, 766, 1822, 1892, 2591, 2694, 2822
MbiI CCGCTC 1 cut(s) 1164
MboI GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
MfeI CAATTG 2 cut(s) 1157, 2532
MflI RGATCY 4 cut(s) 466, 1820, 1890, 2589
MhlI GDGCHC 2 cut(s) 154, 2025
MlsI TGGCCA 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 636
MlyI GAGTC 7 cut(s) 174, 357, 1731, 1803, 2478, 2503, 2515
MmeI TCCRAC 2 cut(s) 887, 2319
Mox20I TGGCCA 1 cut(s) 636
MroI TCCGGA 2 cut(s) 214, 2767
MroXI GAANNNNTTC 1 cut(s) 2008
MscI TGGCCA 1 cut(s) 636
MseI TTAA 6 cut(s) 21, 243, 948, 983, 2613, 2808
MslI CAYNNNNRTG 3 cut(s) 777, 1673, 2388
Msp20I TGGCCA 1 cut(s) 636
MspA1I CMGCKG 1 cut(s) 1469
MspI CCGG 4 cut(s) 37, 215, 1886, 2768
MspR9I CCNGG 2 cut(s) 833, 1887
MunI CAATTG 2 cut(s) 1157, 2532
MvaI CCWGG 1 cut(s) 833
MwoI GCNNNNNNNGC 5 cut(s) 50, 59, 1964, 2462, 2756
NciI CCSGG 1 cut(s) 1887
NcoI CCATGG 2 cut(s) 271, 1804
NdeI CATATG 1 cut(s) 574
NdeII GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
NlaIV GGNNCC 7 cut(s) 205, 370, 421, 493, 1822, 2285, 2683
NmuCI GTSAC 4 cut(s) 74, 139, 609, 2201
NspI RCATGY 4 cut(s) 2033, 2275, 2462, 2788
OliI CACNNNNGTG 2 cut(s) 777, 1673
PagI TCATGA 2 cut(s) 342, 2695
PceI AGGCCT 1 cut(s) 729
PciI ACATGT 1 cut(s) 2458
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 2008
PflMI CCANNNNNTGG 1 cut(s) 1926
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 8 cut(s) 7, 1471, 1760, 1915, 1966, 1969, 2234, 2845
PleI GAGTC 7 cut(s) 173, 356, 1731, 1803, 2478, 2503, 2515
PpsI GAGTC 7 cut(s) 173, 356, 1731, 1803, 2478, 2503, 2515
PpuMI RGGWCCY 1 cut(s) 369
PscI ACATGT 1 cut(s) 2458
Psp124BI GAGCTC 1 cut(s) 2025
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 831
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 7 cut(s) 205, 370, 421, 493, 1822, 2285, 2683
PspPI GGNCC 5 cut(s) 369, 563, 1457, 1601, 1763
PspPPI RGGWCCY 1 cut(s) 369
PsrI GAACNNNNNNTAC 2 cut(s) 170, 202
PstI CTGCAG 4 cut(s) 763, 1969, 2237, 2845
PstNI CAGNNNCTG 2 cut(s) 1472, 2232
PsuI RGATCY 4 cut(s) 466, 1820, 1890, 2589
PvuII CAGCTG 1 cut(s) 1469
RsaI GTAC 4 cut(s) 1317, 1536, 2375, 2397
RsaNI GTAC 4 cut(s) 1316, 1535, 2374, 2396
RseI CAYNNNNRTG 3 cut(s) 777, 1673, 2388
SacI GAGCTC 1 cut(s) 2025
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 6 cut(s) 21, 243, 948, 983, 2613, 2808
SatI GCNGC 8 cut(s) 6, 1470, 1759, 1914, 1965, 1968, 2233, 2844
Sau3AI GATC 9 cut(s) 90, 466, 532, 764, 1820, 1890, 2589, 2692, 2820
Sau96I GGNCC 5 cut(s) 369, 563, 1457, 1601, 1763
SchI GAGTC 7 cut(s) 174, 357, 1731, 1803, 2478, 2503, 2515
ScrFI CCNGG 2 cut(s) 833, 1887
SduI GDGCHC 2 cut(s) 154, 2025
SfaNI GCATC 8 cut(s) 919, 1006, 1061, 1723, 2369, 2629, 2635, 2656
SfcI CTRYAG 5 cut(s) 759, 1965, 2233, 2796, 2841
SinI GGWCC 3 cut(s) 369, 563, 1763
SmiMI CAYNNNNRTG 3 cut(s) 777, 1673, 2388
SmlI CTYRAG 4 cut(s) 325, 541, 1709, 1989
SmoI CTYRAG 4 cut(s) 325, 541, 1709, 1989
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 6 cut(s) 503, 1164, 1758, 1761, 1913, 2642
SspI AATATT 1 cut(s) 952
SstI GAGCTC 1 cut(s) 2025
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 2 cut(s) 831, 1885
StyI CCWWGG 4 cut(s) 271, 730, 1741, 1804
TaaI ACNGT 2 cut(s) 563, 2867
TaiI ACGT 1 cut(s) 2121
TaqI TCGA 4 cut(s) 163, 767, 1146, 1657
TauI GCSGC 2 cut(s) 1761, 1916
Tru1I TTAA 6 cut(s) 21, 243, 948, 983, 2613, 2808
Tru9I TTAA 6 cut(s) 21, 243, 948, 983, 2613, 2808
TscAI CASTG 5 cut(s) 79, 784, 1111, 1675, 2556
TseFI GTSAC 4 cut(s) 74, 139, 609, 2201
TseI GCWGC 6 cut(s) 5, 1469, 1964, 1967, 2232, 2843
Tsp45I GTSAC 4 cut(s) 74, 139, 609, 2201
TspDTI ATGAA 7 cut(s) 331, 1266, 2001, 2064, 2369, 2406, 2584
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 5 cut(s) 79, 784, 1111, 1675, 2556
Van91I CCANNNNNTGG 1 cut(s) 1926
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 3 cut(s) 369, 563, 1763
XapI RAATTY 8 cut(s) 595, 893, 979, 1117, 1381, 1720, 1749, 2311
XbaI TCTAGA 5 cut(s) 535, 826, 1594, 1690, 2635
XceI RCATGY 4 cut(s) 2033, 2275, 2462, 2788
XcmI CCANNNNNNNNNTGG 2 cut(s) 1401, 1811
XmiI GTMKAC 1 cut(s) 2540
XmnI GAANNNNTTC 1 cut(s) 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.