Rw6G018200

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006431
Physical Location & Seq
Reverse (-)
36083027 .. 36086040
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018200.1

Sequence Viewer

Length: 1005 bp
ATGGTTCTGCGAGTTCACAGATGTTGCCTGGATGAAGAAGCCAAGTTCAAAAACCTCGATCAGTTTATGCGGAATTTACTAGGTATTGATGGGATCAGAAGGCTTTTGTTCTCTGCTTTAGGCATGGAAGGTGAACAAAGAGGCATATGTGACCTTTACGTGATGGCGATGTCATCCGATTTGGGATGTAAGTCAACTCGATTATACCAATTCCAAGCACCTTCTGAGTATAAGCACATTCAGGAGACTGATGCTCCAGTAAGACTCCAAATACAATTGCTGAGAAGTGGATCTACACCGTTGGATGCTGATAGCTTGACTTCTGAGAGCAATTGGACTTTTGACCATGTCACAAAACCGTGGATTTCGAAGACTCATTTGGCAAATGCTGTCAAAGTTGATGACATCAGGAGATTTTTGGTGTTGCAACATGTTGAAAAGCTGGATCTTCCGTTTATAGCTATGTGTAGGAGGGAGGATTGCCCGAGTTTATTCAAAGATCCTGATCAACTTGAAGATTATCCTCCATATCAGGATAAAAGTGAGCTAAACTGGCACAAGATGCTTTGGGCCATTCAAGATTTGGACCTCGAATGGCTCATGCTCCAGAAGCGGAAGCGGGAATTGAAAGATCGTTGTCAGAAGTTCTTTGACGAATCACGAAGGCCACATGATAAGATGAGACAGTCATTATATAAGAAGATTTTTCAATCTTCTATGAAGTTCCTTGAGAGTGCTAACTCAGAGAGAGAAGTTGAGGACGTTGAAACAAAATTCATATTCCATTTCAATCAAGATGTGTTACCTCTTGAGCTACATAATGTCGCTGAGGAAGATATTTGGAAGCAAGTGTGGGAAGCATGCCAGTATAGGGTGGAATCAGTTTATAGCGTCTGCAGCAAGGCTGGCCTGTGGAGGGTTGCATGCAACTTCGGTCCTAGCTCTGAAGAACTTGGGTTGCAATTATCAGGGGAAATGGCTATCAAGGAAGACAATGTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

334

Amino Acids

39.2

Weight (kDa)

5.59

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_44 PF14641 100 - 215 4.3e-06 Helix-turn-helix DNA-binding domain of SPT6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 70, 613, 619
AclWI GGATC 4 cut(s) 101, 298, 453, 494
AcsI RAATTY 2 cut(s) 73, 773
AcuI CTGAAG 1 cut(s) 966
AfiI CCNNNNNNNGG 2 cut(s) 871, 916
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 9 cut(s) 49, 437, 496, 515, 578, 628, 710, 767, 790
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 6 cut(s) 315, 442, 461, 547, 814, 942
AluI AGCT 6 cut(s) 315, 442, 461, 547, 814, 942
Alw26I GTCTC 2 cut(s) 239, 676
AlwI GGATC 4 cut(s) 101, 298, 453, 494
Ama87I CYCGRG 1 cut(s) 484
AoxI GGCC 4 cut(s) 570, 665, 907, 999
ApeKI GCWGC 1 cut(s) 897
ApoI RAATTY 2 cut(s) 73, 773
AspS9I GGNCC 3 cut(s) 570, 586, 935
AsuHPI GGTGA 1 cut(s) 143
AsuII TTCGAA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 2 cut(s) 586, 935
BarI GAAGNNNNNNTAC 2 cut(s) 277, 309
BbsI GAAGAC 2 cut(s) 377, 996
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 1 cut(s) 909
BccI CCATC 2 cut(s) 83, 157
BciT130I CCWGG 1 cut(s) 29
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 239, 676
BfaI CTAG 2 cut(s) 80, 939
BfmI CTRYAG 1 cut(s) 895
BisI GCNGC 1 cut(s) 898
BlsI GCNGC 1 cut(s) 899
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 2 cut(s) 586, 935
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 3 cut(s) 570, 586, 935
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 241, 295, 552
BpiI GAAGAC 2 cut(s) 377, 996
BpmI CTGGAG 2 cut(s) 240, 590
Bpu10I CCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 2 cut(s) 749, 830
BsaAI YACGTR 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 2 cut(s) 871, 916
Bse1I ACTGG 3 cut(s) 257, 557, 865
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 359
BseGI GGATG 4 cut(s) 37, 173, 191, 310
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 2 cut(s) 871, 916
BseMII CTCAG 5 cut(s) 216, 272, 315, 756, 819
BseNI ACTGG 3 cut(s) 257, 557, 865
BseXI GCAGC 1 cut(s) 909
BshFI GGCC 4 cut(s) 572, 667, 909, 1001
BsiHKCI CYCGRG 1 cut(s) 484
BslI CCNNNNNNNGG 2 cut(s) 871, 916
BsmAI GTCTC 2 cut(s) 239, 676
BsnI GGCC 4 cut(s) 572, 667, 909, 1001
BsoBI CYCGRG 1 cut(s) 484
Bsp119I TTCGAA 1 cut(s) 368
Bsp143I GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
BspACI CCGC 3 cut(s) 70, 613, 619
BspANI GGCC 4 cut(s) 572, 667, 909, 1001
BspCNI CTCAG 5 cut(s) 217, 273, 316, 755, 820
BspMAI CTGCAG 1 cut(s) 899
BspPI GGATC 4 cut(s) 101, 298, 453, 494
BspT104I TTCGAA 1 cut(s) 368
BsrI ACTGG 3 cut(s) 257, 557, 865
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 300, 360, 687
BstAPI GCANNNNNTGC 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 862, 907, 925
BstDEI CTNAG 5 cut(s) 225, 281, 324, 742, 828
BstDSI CCRYGG 1 cut(s) 359
BstF5I GGATG 4 cut(s) 37, 173, 191, 310
BstKTI GATC 7 cut(s) 61, 96, 293, 448, 502, 508, 634
BstMAI GTCTC 2 cut(s) 239, 676
BstMBI GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
BstMWI GCNNNNNNNGC 5 cut(s) 553, 562, 610, 897, 906
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 3 cut(s) 434, 864, 927
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 895
BstV1I GCAGC 1 cut(s) 909
BstV2I GAAGAC 2 cut(s) 377, 996
BstX2I RGATCY 3 cut(s) 290, 445, 499
BstYI RGATCY 3 cut(s) 290, 445, 499
BsuRI GGCC 4 cut(s) 572, 667, 909, 1001
BtgI CCRYGG 1 cut(s) 359
BtgZI GCGATG 1 cut(s) 182
BtsCI GGATG 4 cut(s) 37, 173, 191, 310
Cac8I GCNNGC 3 cut(s) 862, 907, 925
Cfr13I GGNCC 3 cut(s) 570, 586, 935
CseI GACGC 1 cut(s) 880
CviAII CATG 7 cut(s) 124, 347, 431, 601, 671, 861, 924
DdeI CTNAG 5 cut(s) 225, 281, 324, 742, 828
DpnI GATC 7 cut(s) 60, 95, 292, 447, 501, 507, 633
DpnII GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
Eco47I GGWCC 2 cut(s) 586, 935
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 27
FaeI CATG 7 cut(s) 127, 350, 434, 604, 674, 864, 927
FatI CATG 7 cut(s) 123, 346, 430, 600, 670, 860, 923
FauI CCCGC 1 cut(s) 612
FauNDI CATATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 898
FokI GGATG 4 cut(s) 44, 160, 198, 317
Fsp4HI GCNGC 1 cut(s) 898
FspBI CTAG 2 cut(s) 80, 939
GluI GCNGC 1 cut(s) 898
GsuI CTGGAG 2 cut(s) 240, 590
HaeIII GGCC 4 cut(s) 572, 667, 909, 1001
HgaI GACGC 1 cut(s) 880
Hin1II CATG 7 cut(s) 127, 350, 434, 604, 674, 864, 927
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 4 cut(s) 264, 373, 656, 878
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 3 cut(s) 16, 134, 195
Hpy188I TCNGA 7 cut(s) 98, 178, 226, 325, 642, 745, 946
Hpy188III TCNNGA 9 cut(s) 242, 409, 503, 533, 578, 607, 660, 794, 809
Hpy8I GTNNAC 3 cut(s) 16, 134, 195
HpyAV CCTTC 4 cut(s) 93, 122, 231, 657
HpyCH4III ACNGT 3 cut(s) 300, 360, 687
HpyCH4IV ACGT 2 cut(s) 159, 762
HpyCH4V TGCA 5 cut(s) 427, 897, 923, 927, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 553, 562, 610, 897, 906
HpyF3I CTNAG 5 cut(s) 225, 281, 324, 742, 828
HpySE526I ACGT 2 cut(s) 159, 762
Hsp92II CATG 7 cut(s) 127, 350, 434, 604, 674, 864, 927
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
LmnI GCTCC 2 cut(s) 259, 609
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 3 cut(s) 241, 295, 552
MaeI CTAG 2 cut(s) 80, 939
MaeII ACGT 2 cut(s) 159, 762
MaeIII GTNAC 3 cut(s) 149, 349, 801
MalI GATC 7 cut(s) 60, 95, 292, 447, 501, 507, 633
MboI GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
MboII GAAGA 9 cut(s) 47, 382, 440, 527, 705, 712, 845, 959, 1001
MfeI CAATTG 2 cut(s) 275, 331
MflI RGATCY 3 cut(s) 290, 445, 499
MluCI AATT 7 cut(s) 73, 209, 275, 331, 623, 773, 962
MlyI GAGTC 2 cut(s) 258, 367
MmeI TCCRAC 1 cut(s) 282
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 2 cut(s) 275, 331
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 5 cut(s) 553, 562, 610, 897, 906
NdeI CATATG 1 cut(s) 146
NdeII GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
NlaIII CATG 7 cut(s) 127, 350, 434, 604, 674, 864, 927
NmuCI GTSAC 2 cut(s) 149, 349
NspI RCATGY 3 cut(s) 434, 864, 927
NspV TTCGAA 1 cut(s) 368
PaeI GCATGC 2 cut(s) 864, 927
PciI ACATGT 1 cut(s) 430
PfeI GAWTC 2 cut(s) 656, 878
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 1 cut(s) 899
PleI GAGTC 2 cut(s) 258, 367
PpsI GAGTC 2 cut(s) 258, 367
Ppu21I YACGTR 1 cut(s) 160
PscI ACATGT 1 cut(s) 430
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspPI GGNCC 3 cut(s) 570, 586, 935
PstI CTGCAG 1 cut(s) 899
PsuI RGATCY 3 cut(s) 290, 445, 499
PsyI GACNNNGTC 1 cut(s) 347
SatI GCNGC 1 cut(s) 898
Sau3AI GATC 7 cut(s) 58, 93, 290, 445, 499, 505, 631
Sau96I GGNCC 3 cut(s) 570, 586, 935
SchI GAGTC 2 cut(s) 258, 367
ScrFI CCNGG 1 cut(s) 29
SfaNI GCATC 3 cut(s) 241, 295, 552
SfcI CTRYAG 1 cut(s) 895
SfuI TTCGAA 1 cut(s) 368
SinI GGWCC 2 cut(s) 586, 935
SmlI CTYRAG 2 cut(s) 728, 809
SmoI CTYRAG 2 cut(s) 728, 809
SphI GCATGC 2 cut(s) 864, 927
Sse9I AATT 7 cut(s) 73, 209, 275, 331, 623, 773, 962
SsiI CCGC 3 cut(s) 70, 613, 619
SspMI CTAG 2 cut(s) 80, 939
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 300, 360, 687
TaiI ACGT 2 cut(s) 162, 765
TaqI TCGA 4 cut(s) 57, 199, 368, 591
TaqII GACCGA 1 cut(s) 923
TasI AATT 7 cut(s) 73, 209, 275, 331, 623, 773, 962
TfiI GAWTC 2 cut(s) 656, 878
TseFI GTSAC 2 cut(s) 149, 349
TseI GCWGC 1 cut(s) 897
Tsp45I GTSAC 2 cut(s) 149, 349
TspDTI ATGAA 3 cut(s) 48, 734, 766
TspGWI ACGGA 1 cut(s) 441
Tth111I GACNNNGTC 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 586, 935
XapI RAATTY 2 cut(s) 73, 773
XceI RCATGY 3 cut(s) 434, 864, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 580
XspI CTAG 2 cut(s) 80, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.