Rroxscaffold_4G00311770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006431
Physical Location & Seq
Forward (+)
34340616 .. 34348990
8375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311770.1

Sequence Viewer

Length: 411 bp
ATGCACAATCATAAAGATTATAACAATGGGCATCCTTGGATCTTCACCAAATTACCACGATCCTTCTTCAATCAAAAATATGAACACCGGCCTTGTATCCTCCTCGGTGCGATCGATCGAACCCAAACATCCCTCCGCTTAATCTCTCCGTCTCCGGCGGCGGCGGTGCGAGCTGTACATCCCTCCTCCCTCGTTCTCTTCCACCGCGCCGGCCGAAGGCACTTCATTTACACTCCTTTTCGCTCTCGCTGGACACAATTTCCTACCGGACCTCCTCCACCGTCTTCGGCCTCGGCGGAGGCGCTCGCTGTTGTAGACGTCGCGGAAGAAGCCGTCGACGATCATCGAGCCCACCGGATCGGCGTGGGATTGGCCGTCTACATTTCCACCACCAACCTTTCGGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.23

Weight (kDa)

9.88

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AatII GACGTC 1 cut(s) 321
AccI GTMKAC 3 cut(s) 315, 336, 378
AccII CGCG 2 cut(s) 207, 323
AciI CCGC 7 cut(s) 136, 158, 161, 164, 205, 296, 323
AclWI GGATC 4 cut(s) 47, 54, 365, 398
AcoI YGGCCR 2 cut(s) 211, 372
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 1 cut(s) 70
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 4 cut(s) 47, 54, 365, 398
AoxI GGCC 4 cut(s) 89, 211, 288, 372
AspLEI GCGC 2 cut(s) 209, 304
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 317, 359
BciVI GTATCC 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 409
BfoI RGCGCY 1 cut(s) 305
BfuI GTATCC 1 cut(s) 107
BisI GCNGC 2 cut(s) 159, 162
BlsI GCNGC 2 cut(s) 160, 163
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 405
BmsI GCATC 1 cut(s) 40
BpiI GAAGAC 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 35, 103, 291
BsaWI WCCGGW 2 cut(s) 266, 354
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse118I RCCGGY 2 cut(s) 87, 209
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 35, 103, 291
BseGI GGATG 3 cut(s) 31, 128, 178
BseLI CCNNNNNNNGG 1 cut(s) 216
BseRI GAGGAG 3 cut(s) 92, 175, 264
BseX3I CGGCCG 1 cut(s) 211
Bsh1236I CGCG 2 cut(s) 207, 323
Bsh1285I CGRYCG 3 cut(s) 114, 118, 214
BshFI GGCC 4 cut(s) 91, 213, 290, 374
BshVI ATCGAT 1 cut(s) 114
BsiEI CGRYCG 3 cut(s) 114, 118, 214
BsiSI CCGG 5 cut(s) 88, 155, 210, 267, 355
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 1 cut(s) 156
BsmBI CGTCTC 1 cut(s) 156
BsnI GGCC 4 cut(s) 91, 213, 290, 374
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
BspACI CCGC 7 cut(s) 136, 158, 161, 164, 205, 296, 323
BspANI GGCC 4 cut(s) 91, 213, 290, 374
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 2 cut(s) 207, 323
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 4 cut(s) 47, 54, 365, 398
BsrFI RCCGGY 2 cut(s) 87, 209
BsrGI TGTACA 1 cut(s) 175
BssAI RCCGGY 2 cut(s) 87, 209
BssECI CCNNGG 3 cut(s) 35, 103, 291
BssMI GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 282
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 3 cut(s) 171, 211, 306
BstF5I GGATG 3 cut(s) 31, 128, 178
BstFNI CGCG 2 cut(s) 207, 323
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 209, 304
BstKTI GATC 7 cut(s) 42, 62, 114, 118, 343, 360, 406
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
BstMCI CGRYCG 3 cut(s) 114, 118, 214
BstMWI GCNNNNNNNGC 2 cut(s) 170, 329
BstUI CGCG 2 cut(s) 207, 323
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 39, 403
BstYI RGATCY 2 cut(s) 39, 403
BstZI CGGCCG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 114
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 4 cut(s) 91, 213, 290, 374
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 3 cut(s) 31, 128, 178
Cac8I GCNNGC 3 cut(s) 171, 211, 306
CfoI GCGC 2 cut(s) 209, 304
Cfr10I RCCGGY 2 cut(s) 87, 209
Cfr13I GGNCC 1 cut(s) 269
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 1 cut(s) 176
CviJI RGCY 7 cut(s) 91, 173, 213, 290, 332, 350, 374
CviKI_1 RGCY 7 cut(s) 91, 173, 213, 290, 332, 350, 374
CviQI GTAC 1 cut(s) 176
DpnI GATC 7 cut(s) 41, 61, 113, 117, 342, 359, 405
DpnII GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
EaeI YGGCCR 2 cut(s) 211, 372
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 269
Eco52I CGGCCG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 35
Esp3I CGTCTC 1 cut(s) 156
FaiI YATR 3 cut(s) 12, 21, 81
FblI GTMKAC 3 cut(s) 315, 336, 378
Fnu4HI GCNGC 2 cut(s) 159, 162
FokI GGATG 3 cut(s) 18, 115, 165
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 2 cut(s) 159, 162
FspBI CTAG 1 cut(s) 409
GlaI GCGC 2 cut(s) 208, 303
GluI GCNGC 2 cut(s) 159, 162
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 4 cut(s) 91, 213, 290, 374
HapII CCGG 5 cut(s) 88, 155, 210, 267, 355
HhaI GCGC 2 cut(s) 209, 304
Hin1I GRCGYC 1 cut(s) 318
Hin6I GCGC 2 cut(s) 207, 302
HinP1I GCGC 2 cut(s) 207, 302
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HpaII CCGG 5 cut(s) 88, 155, 210, 267, 355
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 3 cut(s) 316, 337, 379
Hpy188I TCNGA 1 cut(s) 403
Hpy8I GTNNAC 3 cut(s) 316, 337, 379
Hpy99I CGWCG 3 cut(s) 323, 338, 341
HpyAV CCTTC 2 cut(s) 73, 210
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 329
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 318
HspAI GCGC 2 cut(s) 207, 302
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
LpnPI CCDG 6 cut(s) 101, 168, 223, 235, 280, 368
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 409
MaeII ACGT 1 cut(s) 318
MalI GATC 7 cut(s) 41, 61, 113, 117, 342, 359, 405
MboI GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
MboII GAAGA 5 cut(s) 34, 58, 190, 276, 338
MflI RGATCY 2 cut(s) 39, 403
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 2 cut(s) 50, 257
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 1 cut(s) 140
MspI CCGG 5 cut(s) 88, 155, 210, 267, 355
MvnI CGCG 2 cut(s) 207, 323
MwoI GCNNNNNNNGC 2 cut(s) 170, 329
NaeI GCCGGC 1 cut(s) 211
NdeII GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
NgoMIV GCCGGC 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 405
NmeAIII GCCGAG 1 cut(s) 272
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdiI GCCGGC 1 cut(s) 211
PkrI GCNGC 2 cut(s) 160, 163
Ple19I CGATCG 2 cut(s) 114, 118
PsiI TTATAA 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 269
PsuI RGATCY 2 cut(s) 39, 403
PvuI CGATCG 2 cut(s) 114, 118
RsaI GTAC 1 cut(s) 177
RsaNI GTAC 1 cut(s) 176
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 2 cut(s) 159, 162
Sau3AI GATC 7 cut(s) 39, 59, 111, 115, 340, 357, 403
Sau96I GGNCC 1 cut(s) 269
SduI GDGCHC 1 cut(s) 352
SetI ASST 4 cut(s) 175, 274, 321, 399
SfaNI GCATC 1 cut(s) 40
SgrDI CGTCGACG 1 cut(s) 335
SinI GGWCC 1 cut(s) 269
Sse9I AATT 2 cut(s) 50, 257
SsiI CCGC 7 cut(s) 136, 158, 161, 164, 205, 296, 323
SspMI CTAG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 282
TaiI ACGT 1 cut(s) 321
TaqI TCGA 4 cut(s) 114, 118, 336, 346
TasI AATT 2 cut(s) 50, 257
TatI WGTACW 1 cut(s) 175
TauI GCSGC 2 cut(s) 161, 164
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 96, 214
TspGWI ACGGA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 269
XmiI GTMKAC 3 cut(s) 315, 336, 378
XspI CTAG 1 cut(s) 409
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.