Rroxscaffold_2G00138830

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005561
Physical Location & Seq
Reverse (-)
77032444 .. 77033909
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138830.1

Sequence Viewer

Length: 378 bp
ATGGTTGGTGGTGACGGAGTGTGGGTTAGTGGTACTGTCACCGGCGACCACCGCTCCGCCCTACTCCAAGTCATCGGCATAGTCCCCGCCTCGCCGACTCCGACGACCTTTGGCCCAACCAAAGCTTCTACGTCCGGCCTCTCCGACTCCCTCAACTCCACCTACGTCTCCCTCTCCGAGAAAGACACCGATCTCATCCTCGCCAACCGCCTCCTTCGTCCGGCTCGGCCGGTTCGTCTACGTCGACCGCTTCGATTTCGACTCTCCGTGCGGCGCGTCGCCGAATCGCCCTCATCGCCGGCCGCCACCCCTTCGTTGGTTCGCCGGAGCCTCGGTGGCGGATTTCTCCGAGGAAAAGATTGTCCGTCGGGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

125

Amino Acids

13.18

Weight (kDa)

11.27

Isoelectric Point (pI)

71.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF936 PF06075 12 - 71 1.9e-13 Plant protein of unknown function (DUF936)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 54
AccI GTMKAC 2 cut(s) 238, 244
AccII CGCG 1 cut(s) 276
AciI CCGC 8 cut(s) 52, 57, 87, 208, 248, 271, 303, 339
AcoI YGGCCR 2 cut(s) 227, 300
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 220, 316
AjuI GAANNNNNNNTTGG 2 cut(s) 109, 141
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 172
AoxI GGCC 4 cut(s) 112, 136, 227, 300
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 23, 31
BcoDI GTCTC 1 cut(s) 172
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 2 cut(s) 272, 303
BlsI GCNGC 2 cut(s) 273, 304
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 1 cut(s) 329
BsaJI CCNNGG 2 cut(s) 331, 349
BsaXI ACNNNNNCTCC 6 cut(s) 9, 38, 39, 68, 319, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 316
Bse118I RCCGGY 3 cut(s) 41, 229, 298
BseDI CCNNGG 2 cut(s) 331, 349
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 220, 316
BseX3I CGGCCG 2 cut(s) 227, 300
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 3 cut(s) 230, 248, 303
BshFI GGCC 4 cut(s) 114, 138, 229, 302
BsiEI CGRYCG 3 cut(s) 230, 248, 303
BsiSI CCGG 6 cut(s) 42, 135, 221, 230, 299, 325
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 220, 316
BsmAI GTCTC 1 cut(s) 172
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 4 cut(s) 114, 138, 229, 302
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 8 cut(s) 52, 57, 87, 208, 248, 271, 303, 339
BspANI GGCC 4 cut(s) 114, 138, 229, 302
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 329
BsrBI CCGCTC 1 cut(s) 54
BsrFI RCCGGY 3 cut(s) 41, 229, 298
BssAI RCCGGY 3 cut(s) 41, 229, 298
BssECI CCNNGG 2 cut(s) 331, 349
BssMI GATC 1 cut(s) 190
Bst4CI ACNGT 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 300
BstF5I GGATG 1 cut(s) 195
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 1 cut(s) 190
BstMCI CGRYCG 3 cut(s) 230, 248, 303
BstMWI GCNNNNNNNGC 3 cut(s) 51, 295, 336
BstUI CGCG 1 cut(s) 276
BstZI CGGCCG 2 cut(s) 227, 300
BsuRI GGCC 4 cut(s) 114, 138, 229, 302
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 3 cut(s) 41, 229, 298
Cfr13I GGNCC 1 cut(s) 113
CseI GACGC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 33
CviJI RGCY 7 cut(s) 114, 125, 138, 224, 229, 302, 330
CviKI_1 RGCY 7 cut(s) 114, 125, 138, 224, 229, 302, 330
CviQI GTAC 1 cut(s) 33
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 2 cut(s) 227, 300
EagI CGGCCG 2 cut(s) 227, 300
EciI GGCGGA 2 cut(s) 46, 354
EclXI CGGCCG 2 cut(s) 227, 300
Eco52I CGGCCG 2 cut(s) 227, 300
Esp3I CGTCTC 1 cut(s) 172
FaiI YATR 1 cut(s) 80
FaqI GGGAC 1 cut(s) 68
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 2 cut(s) 238, 244
Fnu4HI GCNGC 2 cut(s) 272, 303
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 272, 303
GlaI GCGC 1 cut(s) 275
GluI GCNGC 2 cut(s) 272, 303
HaeIII GGCC 4 cut(s) 114, 138, 229, 302
HapII CCGG 6 cut(s) 42, 135, 221, 230, 299, 325
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 276
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 245
HindII GTYRAC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 4 cut(s) 97, 146, 261, 284
HpaII CCGG 6 cut(s) 42, 135, 221, 230, 299, 325
HphI GGTGA 2 cut(s) 23, 31
Hpy166II GTNNAC 2 cut(s) 239, 245
Hpy188I TCNGA 4 cut(s) 102, 145, 178, 350
Hpy8I GTNNAC 2 cut(s) 239, 245
Hpy99I CGWCG 4 cut(s) 106, 246, 281, 370
HpyAV CCTTC 2 cut(s) 224, 321
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 3 cut(s) 131, 165, 241
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 295, 336
HpySE526I ACGT 3 cut(s) 131, 165, 241
HspAI GCGC 1 cut(s) 274
KroI GCCGGC 1 cut(s) 298
KroNI GCCGGC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 2 cut(s) 59, 327
LpnPI CCDG 6 cut(s) 55, 148, 234, 243, 312, 338
MaeII ACGT 3 cut(s) 131, 165, 241
MaeIII GTNAC 2 cut(s) 11, 37
MalI GATC 1 cut(s) 192
MbiI CCGCTC 1 cut(s) 54
MboI GATC 1 cut(s) 190
MlyI GAGTC 3 cut(s) 91, 140, 255
MmeI TCCRAC 2 cut(s) 125, 168
MnlI CCTC 9 cut(s) 100, 149, 161, 182, 209, 221, 301, 341, 344
MroNI GCCGGC 1 cut(s) 298
MspI CCGG 6 cut(s) 42, 135, 221, 230, 299, 325
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 51, 295, 336
NaeI GCCGGC 1 cut(s) 300
NdeII GATC 1 cut(s) 190
NgoMIV GCCGGC 1 cut(s) 298
NlaIV GGNNCC 1 cut(s) 329
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 11, 37
PcsI WCGNNNNNNNCGW 4 cut(s) 98, 232, 241, 250
PdiI GCCGGC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 2 cut(s) 273, 304
PleI GAGTC 3 cut(s) 91, 140, 255
PpsI GAGTC 3 cut(s) 91, 140, 255
PspN4I GGNNCC 1 cut(s) 329
PspPI GGNCC 1 cut(s) 113
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SalI GTCGAC 1 cut(s) 243
SatI GCNGC 2 cut(s) 272, 303
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 3 cut(s) 91, 140, 255
SetI ASST 6 cut(s) 110, 127, 134, 164, 168, 244
SgrAI CRCCGGYG 1 cut(s) 41
SsiI CCGC 8 cut(s) 52, 57, 87, 208, 248, 271, 303, 339
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 3 cut(s) 134, 168, 244
TaqI TCGA 3 cut(s) 244, 253, 259
TauI GCSGC 2 cut(s) 274, 305
TfiI GAWTC 1 cut(s) 284
TseFI GTSAC 2 cut(s) 11, 37
Tsp45I GTSAC 2 cut(s) 11, 37
TspGWI ACGGA 3 cut(s) 30, 256, 354
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XmiI GTMKAC 2 cut(s) 238, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.