Rroxscaffold_5G00365000

Phylloplanin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002452
Physical Location & Seq
Forward (+)
46263924 .. 46271694
7771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365000.1

Sequence Viewer

Length: 666 bp
ATGGCGCCCTCCAAAAAGACAATGTTGTTTGTGTGCCTCTTGGTTGCTGCAGTGGCAGTAGTTGTTCCGATAGCTCAAGGGCAGCTGAACTTTGGAGACCTCATCGCGCCTCTTCTTCGTCTCGTCAGCATCCAAGGCACAGTCTTTTGCAGTGCCAATGGTCGTGCCACCGTTGGCACCATTCTTACCCCACCATTTGCAAACGCTACGGTCCAACTACGCTGCGGAGCAGGAAATGGTACTGTGATTGCAACTGCGCAGACCAACTCCAACGGAGTGTTTTCAATCGTGTTAGACCCTTCCTCCATTACAATACCTCAAATATTGTCTGGATGCCGTGCTGTGGTTACAACACCACTTGTCAGTTGCAACGCCACCCTCCCTTCAACTGGAACTCTCACATCGACCTTGACATCAATTGGAAACACCACTGTTGGCCTCTTGCCCGTCATCAATTTCAGTGCCGACCTGTTCACTAGCACCGCAATCTGGTTGAGCAAGTTTTCTATGACATTTCCGACATGGGACTCACCTAGTCACCTTTCATTGAGTCCGTTAAGTTGGTTGCAAGCTGGAAACCGGAGATCGAAGGTGTGTGAATGGTGCTACAGCTCCGCCTATGCTTCGCAGTACCGTTCATCTTCGATCGGCAACGATGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

221

Amino Acids

23.07

Weight (kDa)

8.78

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 44 - 126 2.1e-06 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
AccB1I GGYRCC 2 cut(s) 4, 176
AccII CGCG 1 cut(s) 107
AciI CCGC 3 cut(s) 225, 483, 615
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 241, 632
AfiI CCNNNNNNNGG 3 cut(s) 343, 389, 489
AgsI TTSAA 2 cut(s) 285, 387
AluBI AGCT 4 cut(s) 74, 85, 572, 612
AluI AGCT 4 cut(s) 74, 85, 572, 612
Alw26I GTCTC 2 cut(s) 90, 125
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 3 cut(s) 47, 82, 222
AspLEI GCGC 3 cut(s) 7, 109, 259
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 522, 530
AvaII GGWCC 1 cut(s) 211
BanI GGYRCC 2 cut(s) 4, 176
BbvI GCAGC 3 cut(s) 34, 94, 209
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 90, 125
BfaI CTAG 2 cut(s) 477, 534
BfmI CTRYAG 2 cut(s) 48, 607
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 48, 83, 223
BlsI GCNGC 3 cut(s) 49, 84, 224
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 6, 178
BmsI GCATC 2 cut(s) 138, 323
BpuEI CTTGAG 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 389, 489
Bse1I ACTGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 2 cut(s) 129, 338
BseLI CCNNNNNNNGG 3 cut(s) 343, 389, 489
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 3 cut(s) 34, 94, 209
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 648
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 2 cut(s) 4, 176
BsiEI CGRYCG 1 cut(s) 648
BsiSI CCGG 1 cut(s) 580
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 3 cut(s) 343, 389, 489
BsmAI GTCTC 2 cut(s) 90, 125
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 539
BsnI GGCC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 584, 645
BspACI CCGC 3 cut(s) 225, 483, 615
BspANI GGCC 1 cut(s) 438
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 6, 178
BspMAI CTGCAG 1 cut(s) 52
BspT107I GGYRCC 2 cut(s) 4, 176
BspTNI GGTCTC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 584, 645
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 133
Bst4CI ACNGT 6 cut(s) 142, 172, 211, 244, 433, 635
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 570
BstF5I GGATG 2 cut(s) 129, 338
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 109, 259
BstKTI GATC 2 cut(s) 587, 648
BstMAI GTCTC 2 cut(s) 90, 125
BstMBI GATC 2 cut(s) 584, 645
BstMCI CGRYCG 1 cut(s) 648
BstMWI GCNNNNNNNGC 2 cut(s) 53, 135
BstSFI CTRYAG 2 cut(s) 48, 607
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 34, 94, 209
BsuRI GGCC 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 129, 338
BtsI GCAGTG 2 cut(s) 57, 157
BtsIMutI CAGTG 4 cut(s) 57, 157, 429, 466
Cac8I GCNNGC 1 cut(s) 570
CfoI GCGC 3 cut(s) 7, 109, 259
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 240, 631
CviAII CATG 1 cut(s) 522
CviJI RGCY 5 cut(s) 74, 85, 438, 572, 612
CviKI_1 RGCY 5 cut(s) 74, 85, 438, 572, 612
CviQI GTAC 2 cut(s) 240, 631
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 586, 647
DpnII GATC 2 cut(s) 584, 645
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 133
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 133
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 1 cut(s) 525
FaiI YATR 3 cut(s) 509, 523, 621
FaqI GGGAC 1 cut(s) 539
FatI CATG 1 cut(s) 521
Fnu4HI GCNGC 3 cut(s) 48, 83, 223
FokI GGATG 2 cut(s) 116, 345
Fsp4HI GCNGC 3 cut(s) 48, 83, 223
FspBI CTAG 2 cut(s) 477, 534
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 3 cut(s) 6, 108, 258
GluI GCNGC 3 cut(s) 48, 83, 223
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 438
HapII CCGG 1 cut(s) 580
HhaI GCGC 3 cut(s) 7, 109, 259
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 525
Hin6I GCGC 3 cut(s) 5, 107, 257
HinP1I GCGC 3 cut(s) 5, 107, 257
HinfI GANTC 2 cut(s) 527, 550
HpaII CCGG 1 cut(s) 580
HphI GGTGA 2 cut(s) 522, 530
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 69, 519
Hpy188III TCNNGA 1 cut(s) 330
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 3 cut(s) 309, 393, 583
HpyCH4III ACNGT 6 cut(s) 142, 172, 211, 244, 433, 635
HpyCH4V TGCA 6 cut(s) 50, 150, 200, 251, 369, 568
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 135
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 525
HspAI GCGC 3 cut(s) 5, 107, 257
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 584, 645
LmnI GCTCC 2 cut(s) 227, 617
LpnPI CCDG 7 cut(s) 216, 315, 375, 475, 482, 558, 593
Lsp1109I GCAGC 3 cut(s) 34, 94, 209
LweI GCATC 2 cut(s) 138, 323
MaeI CTAG 2 cut(s) 477, 534
MaeIII GTNAC 2 cut(s) 346, 536
MalI GATC 2 cut(s) 586, 647
MboI GATC 2 cut(s) 584, 645
MboII GAAGA 3 cut(s) 104, 107, 633
MfeI CAATTG 1 cut(s) 417
MluCI AATT 2 cut(s) 417, 454
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 521, 559
MmeI TCCRAC 3 cut(s) 238, 294, 542
MnlI CCTC 8 cut(s) 19, 47, 110, 120, 313, 327, 389, 449
MseI TTAA 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 1 cut(s) 580
MunI CAATTG 1 cut(s) 417
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 53, 135
NarI GGCGCC 1 cut(s) 5
NdeII GATC 2 cut(s) 584, 645
NlaIII CATG 1 cut(s) 525
NlaIV GGNNCC 2 cut(s) 6, 178
NmuCI GTSAC 1 cut(s) 536
NsbI TGCGCA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 49, 84, 224
Ple19I CGATCG 1 cut(s) 648
PleI GAGTC 2 cut(s) 521, 558
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 521, 558
PspN4I GGNNCC 2 cut(s) 6, 178
PspPI GGNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 52
PvuI CGATCG 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 85
RsaI GTAC 2 cut(s) 241, 632
RsaNI GTAC 2 cut(s) 240, 631
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 3 cut(s) 48, 83, 223
Sau3AI GATC 2 cut(s) 584, 645
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 2 cut(s) 521, 559
SfaNI GCATC 2 cut(s) 138, 323
SfcI CTRYAG 2 cut(s) 48, 607
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 2 cut(s) 417, 454
SsiI CCGC 3 cut(s) 225, 483, 615
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 324
SspMI CTAG 2 cut(s) 477, 534
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 6 cut(s) 142, 172, 211, 244, 433, 635
TaqI TCGA 3 cut(s) 404, 587, 644
TasI AATT 2 cut(s) 417, 454
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 4 cut(s) 57, 157, 436, 466
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 3 cut(s) 47, 82, 222
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 2 cut(s) 534, 627
TspGWI ACGGA 2 cut(s) 288, 543
TspRI CASTG 4 cut(s) 57, 157, 436, 466
VpaK11BI GGWCC 1 cut(s) 211
XspI CTAG 2 cut(s) 477, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.