Rroxscaffold_5G00365030

Phylloplanin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002452
Physical Location & Seq
Forward (+)
46322454 .. 46326395
3942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365030.1

Sequence Viewer

Length: 582 bp
ATGGCGCCCTCCAAAAAGACAATGTTGTTTGTGTGCCTCTTGGTTGCTGCAGTGGCAGTGGTTGTTCCGATAGCTCAAGGGCAGCTGAACTTTGGAGACCTCATCGCGCCTCTTCTTCGTCTCGTCAGCATCCAAGGCACAGTCTTTTGCAGTGCCAATGGTCGTGCCACCGTTGGCACCATTCTTACCCCACCATTTGCAAACGCTACGGTCCAACTACGTTGCGGAGCAGGAAATGGTACTGTGATTGCAGCTGTGCAGACCAACTCCAACGGAGTGTTTTCAATCGTGTTAGACCCTTCCTCCATTACAATACCTCAAATATTGTCCGGATGCCGTGCTGTGGTTACAACACCACTTGTCAGTTGCAACGCCACCCTCCCTTCAACTGGAACTCTCACATCGACCTTGACATCAATTGGAAATACCACTGTTGGCCTCTTGCCCGTCATCAATTTCAGTGCCGACCTGTTCACTAGCACCAAAATAAGCGGAGCTGTGGAAACGAGAGAAAATAGTGCGAAATTAGAGCAAATTGAAGTTTCGACAAAAGGACGAAATTGTCGACACAATCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

193

Amino Acids

19.88

Weight (kDa)

9.12

Isoelectric Point (pI)

24.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 44 - 126 5.4e-06 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 4, 176
AccI GTMKAC 1 cut(s) 565
AccII CGCG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 2 cut(s) 225, 492
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 2 cut(s) 343, 389
AgsI TTSAA 3 cut(s) 285, 387, 539
AluBI AGCT 4 cut(s) 74, 85, 254, 497
AluI AGCT 4 cut(s) 74, 85, 254, 497
Alw26I GTCTC 2 cut(s) 90, 125
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 3 cut(s) 47, 82, 251
AspLEI GCGC 2 cut(s) 7, 109
AspS9I GGNCC 1 cut(s) 211
AvaII GGWCC 1 cut(s) 211
BanI GGYRCC 2 cut(s) 4, 176
BbvI GCAGC 3 cut(s) 34, 94, 263
BceAI ACGGC 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 90, 125
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 48, 83, 252
BlsI GCNGC 3 cut(s) 49, 84, 253
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 6, 178
BmsI GCATC 2 cut(s) 138, 323
BpuEI CTTGAG 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 389
Bse1I ACTGG 1 cut(s) 394
BseAI TCCGGA 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 2 cut(s) 129, 338
BseLI CCNNNNNNNGG 2 cut(s) 343, 389
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 3 cut(s) 34, 94, 263
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 2 cut(s) 4, 176
BsiSI CCGG 1 cut(s) 330
BslI CCNNNNNNNGG 2 cut(s) 343, 389
BsmAI GTCTC 2 cut(s) 90, 125
BsmBI CGTCTC 1 cut(s) 125
BsnI GGCC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 90
Bsp13I TCCGGA 1 cut(s) 329
BspACI CCGC 2 cut(s) 225, 492
BspANI GGCC 1 cut(s) 438
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 6, 178
BspMAI CTGCAG 1 cut(s) 52
BspT107I GGYRCC 2 cut(s) 4, 176
BspTNI GGTCTC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 133
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 133
Bst4CI ACNGT 5 cut(s) 142, 172, 211, 244, 433
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 5
BstF5I GGATG 2 cut(s) 129, 338
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 109
BstMAI GTCTC 2 cut(s) 90, 125
BstMWI GCNNNNNNNGC 2 cut(s) 53, 135
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 34, 94, 263
BsuRI GGCC 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 129, 338
BtsI GCAGTG 3 cut(s) 57, 63, 157
BtsIMutI CAGTG 5 cut(s) 57, 63, 157, 429, 466
CfoI GCGC 2 cut(s) 7, 109
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 240
CviAII CATG 1 cut(s) 579
CviJI RGCY 5 cut(s) 74, 85, 254, 438, 497
CviKI_1 RGCY 5 cut(s) 74, 85, 254, 438, 497
CviQI GTAC 1 cut(s) 240
DinI GGCGCC 1 cut(s) 6
DrdI GACNNNNNNGTC 1 cut(s) 561
DseDI GACNNNNNNGTC 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 133
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 133
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 1 cut(s) 582
FaiI YATR 1 cut(s) 580
FatI CATG 1 cut(s) 578
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 3 cut(s) 48, 83, 252
FokI GGATG 2 cut(s) 116, 345
Fsp4HI GCNGC 3 cut(s) 48, 83, 252
FspBI CTAG 1 cut(s) 477
GlaI GCGC 2 cut(s) 6, 108
GluI GCNGC 3 cut(s) 48, 83, 252
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 438
HapII CCGG 1 cut(s) 330
HhaI GCGC 2 cut(s) 7, 109
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 582
Hin6I GCGC 2 cut(s) 5, 107
HinP1I GCGC 2 cut(s) 5, 107
HincII GTYRAC 1 cut(s) 566
HindII GTYRAC 1 cut(s) 566
HpaII CCGG 1 cut(s) 330
Hpy166II GTNNAC 2 cut(s) 474, 566
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 1 cut(s) 330
Hpy8I GTNNAC 2 cut(s) 474, 566
HpyAV CCTTC 2 cut(s) 309, 393
HpyCH4III ACNGT 5 cut(s) 142, 172, 211, 244, 433
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 6 cut(s) 50, 150, 200, 251, 259, 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 135
HpySE526I ACGT 1 cut(s) 220
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 582
HspAI GCGC 2 cut(s) 5, 107
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 329
LmnI GCTCC 2 cut(s) 227, 494
LpnPI CCDG 4 cut(s) 216, 343, 375, 482
Lsp1109I GCAGC 3 cut(s) 34, 94, 263
LweI GCATC 2 cut(s) 138, 323
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 1 cut(s) 346
MboII GAAGA 2 cut(s) 104, 107
MfeI CAATTG 1 cut(s) 417
MluCI AATT 5 cut(s) 417, 454, 524, 534, 559
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 238, 294
MnlI CCTC 8 cut(s) 19, 47, 110, 120, 313, 327, 389, 449
MroI TCCGGA 1 cut(s) 329
MspA1I CMGCKG 2 cut(s) 85, 254
MspI CCGG 1 cut(s) 330
MunI CAATTG 1 cut(s) 417
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 53, 135
NarI GGCGCC 1 cut(s) 5
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 2 cut(s) 6, 178
PcsI WCGNNNNNNNCGW 1 cut(s) 562
PkrI GCNGC 3 cut(s) 49, 84, 253
PluTI GGCGCC 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 6, 178
PspPI GGNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 52
PvuII CAGCTG 2 cut(s) 85, 254
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
SalI GTCGAC 1 cut(s) 564
SatI GCNGC 3 cut(s) 48, 83, 252
Sau96I GGNCC 1 cut(s) 211
SetI ASST 9 cut(s) 76, 87, 102, 223, 256, 319, 410, 471, 499
SfaNI GCATC 2 cut(s) 138, 323
SfcI CTRYAG 1 cut(s) 48
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 5 cut(s) 417, 454, 524, 534, 559
SsiI CCGC 2 cut(s) 225, 492
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 324
SspMI CTAG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 5 cut(s) 142, 172, 211, 244, 433
TaiI ACGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 404, 545, 565
TasI AATT 5 cut(s) 417, 454, 524, 534, 559
TscAI CASTG 5 cut(s) 57, 63, 157, 436, 466
TseI GCWGC 3 cut(s) 47, 82, 251
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 5 cut(s) 57, 63, 157, 436, 466
VpaK11BI GGWCC 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.