Rroxscaffold_7G00189830

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002752
Physical Location & Seq
Forward (+)
29659416 .. 29664572
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189830.1

Sequence Viewer

Length: 1200 bp
ATGCGTGTCCGGACACGTGGACGGACACGATCTCCCAAAACCGACACCGACATAGACCACGATTTGAAGGAGATTGCTTCGCTTCTTGAGACCGCTGCGTACTCCAAGGAGGTCAGGCGAGTTGTCCGCGCCGTTAGGCTGACGATGGCGAAGAGGCGGAAGCTGAGTGTCTCTGTGCTCTCGGCGTTTCTCAGTTTCGCCTTTTCGCCGGGATCTGAGGCTCACACCCGGTTGTCCTCCTATCTTCCGAAGGACGATGAGCATGACATGGATGTTGATACTGCAACTTCTGCCACTCAAGTTCCCAAGAAACATGCCTCGCCTGAGCTAGAGATTTACTGCTACTTGCTGGTGCTGATTTTTCTGATTGATCAGAAAAAATACAGTGAGGAGACACTTCTGAACCTTCTGCTTCGCAATTACCTGTCATATAACCTATATGATCAGGCAGAGAAACTGAGGTCCAAGGCTCCCCGATTTGAGGCTCACTCAAACCAGCAGTTTTGTCGGTATCTCTTTTATCTGGGAAAGATCAGAACCATTCAGTTGGAGTATACTGATGCAAAGGAGTCCCTTCTTCAAGCTGCTAGGAAGGCCCCTGTAGCTGCCCTTGGCTTTCGAATTCAATGCAACAAGTGGGCAGTTATCGTACGCTTGTTGCTTGGAGAAATCCCTGAGAGGACTGTGTTTATGCAAAAAGGAATGGAGAAGGCTTTGAGGCCATATTTTGAACTAACAAATGCTGTGAGGATTGGTGACTTGGAACTATTCAGAACTATAGCTGACAAGTTCTCGAATGTGTTTAGCTCAGACGGAACCCACAATTTGATTGTGAGGCTGCGACATAATGTTATAAGAACAGGGTTGCCTGAGATTGGACTCCCCAACCCTGTTGCTGATGCTGAAAGTATTGTGGCCAAGGCAATCCGAGATGGTGCTATTGATGCCACTCTGGATCATGCAAATGGATATATGGTGTCAAAGGAAACTGGAGATATCTACTCTACAAATGAGCCGCAGGCTGCATTTGATACAAGGATTGCTTTCTGCCTCAATTTGCACAATGATGCAGTGCGAGCACTAAGGTTTCCACCAAATTCTCACAAGGAGAAAGAAAGTGCTGAGAAAAGGAGGGAGAGACAGCAGCAGGAGCAAGAGCTTGCAAAGCATATAGCTGAGGAGGATGATGATGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
PCI Rpn3_C

Protein Analysis

399

Amino Acids

45.59

Weight (kDa)

8.48

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PSMD3_N PF25573 15 - 130 4.2e-16 PSMD3-like, N-terminal TPR repeats
TPR_PSMD3_N PF25573 130 - 246 2.2e-55 PSMD3-like, N-terminal TPR repeats
PCI PF01399 297 - 328 4.9e-06 PCI domain
Rpn3_C PF08375 332 - 397 3e-27 Proteasome regulatory subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 854
AccI GTMKAC 1 cut(s) 554
AccII CGCG 1 cut(s) 129
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 4 cut(s) 93, 127, 157, 1016
AclWI GGATC 2 cut(s) 220, 963
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 3 cut(s) 621, 1096, 1193
AcvI CACGTG 1 cut(s) 17
AfaI GTAC 2 cut(s) 101, 651
AfiI CCNNNNNNNGG 2 cut(s) 481, 875
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 4 cut(s) 67, 581, 626, 731
AluBI AGCT 8 cut(s) 163, 328, 584, 605, 782, 807, 1159, 1175
AluI AGCT 8 cut(s) 163, 328, 584, 605, 782, 807, 1159, 1175
Alw21I GWGCWC 2 cut(s) 180, 1081
Alw26I GTCTC 4 cut(s) 83, 175, 386, 1132
AlwI GGATC 2 cut(s) 220, 963
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 3 cut(s) 594, 719, 915
ApeKI GCWGC 6 cut(s) 95, 584, 605, 838, 1022, 1144
ApoI RAATTY 3 cut(s) 621, 1096, 1193
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 462, 595
AsuC2I CCSGG 2 cut(s) 210, 229
AsuHPI GGTGA 1 cut(s) 767
AsuII TTCGAA 1 cut(s) 619
AvaII GGWCC 1 cut(s) 462
BalI TGGCCA 1 cut(s) 917
BbrPI CACGTG 1 cut(s) 17
Bbv12I GWGCWC 2 cut(s) 180, 1081
BbvCI CCTCAGC 1 cut(s) 1176
BbvI GCAGC 6 cut(s) 82, 571, 592, 825, 1009, 1156
BccI CCATC 2 cut(s) 139, 926
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 4 cut(s) 488, 522, 609, 643
BclI TGATCA 2 cut(s) 370, 442
BcnI CCSGG 2 cut(s) 210, 229
BcoDI GTCTC 4 cut(s) 83, 175, 386, 1132
BfaI CTAG 2 cut(s) 329, 588
BfmI CTRYAG 2 cut(s) 600, 777
BisI GCNGC 7 cut(s) 96, 585, 606, 839, 1016, 1023, 1145
BlsI GCNGC 7 cut(s) 97, 586, 607, 840, 1017, 1024, 1146
Bme1390I CCNGG 2 cut(s) 210, 229
Bme18I GGWCC 1 cut(s) 462
BmgT120I GGNCC 2 cut(s) 462, 595
BmiI GGNNCC 3 cut(s) 471, 597, 817
BmrFI CCNGG 2 cut(s) 210, 229
BmsI GCATC 4 cut(s) 550, 889, 934, 1057
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 1011
Bpu10I CCTNAGC 2 cut(s) 324, 1176
Bpu14I TTCGAA 1 cut(s) 619
BpuEI CTTGAG 2 cut(s) 107, 282
BpuMI CCSGG 2 cut(s) 210, 229
BsaAI YACGTR 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 4 cut(s) 105, 465, 610, 918
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 875
Bse1I ACTGG 1 cut(s) 994
BseAI TCCGGA 1 cut(s) 9
BseDI CCNNGG 4 cut(s) 105, 465, 610, 918
BseGI GGATG 2 cut(s) 277, 1189
BseLI CCNNNNNNNGG 2 cut(s) 481, 875
BseNI ACTGG 1 cut(s) 994
BseRI GAGGAG 2 cut(s) 404, 1193
BseXI GCAGC 6 cut(s) 82, 571, 592, 825, 1009, 1156
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 596, 721, 917
BsiHKAI GWGCWC 2 cut(s) 180, 1081
BsiSI CCGG 3 cut(s) 10, 209, 229
BsiWI CGTACG 1 cut(s) 649
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 481, 875
BsmAI GTCTC 4 cut(s) 83, 175, 386, 1132
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 3 cut(s) 596, 721, 917
Bso31I GGTCTC 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 180, 1081
Bsp13I TCCGGA 1 cut(s) 9
Bsp143I GATC 6 cut(s) 29, 212, 370, 442, 531, 955
BspACI CCGC 4 cut(s) 93, 127, 157, 1016
BspANI GGCC 3 cut(s) 596, 721, 917
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 471, 597, 817
BspPI GGATC 2 cut(s) 220, 963
BspT104I TTCGAA 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 994
BssECI CCNNGG 4 cut(s) 105, 465, 610, 918
BssMI GATC 6 cut(s) 29, 212, 370, 442, 531, 955
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 4 cut(s) 105, 465, 610, 918
Bst1107I GTATAC 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 386, 685
Bst6I CTCTTC 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 1020, 1077, 1161
BstF5I GGATG 2 cut(s) 277, 1189
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 6 cut(s) 32, 215, 373, 445, 534, 958
BstMAI GTCTC 4 cut(s) 83, 175, 386, 1132
BstMBI GATC 6 cut(s) 29, 212, 370, 442, 531, 955
BstMWI GCNNNNNNNGC 7 cut(s) 290, 593, 602, 944, 1076, 1150, 1165
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 208, 227
BstSFI CTRYAG 2 cut(s) 600, 777
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 6 cut(s) 82, 571, 592, 825, 1009, 1156
BstX2I RGATCY 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 1 cut(s) 212
BstZ17I GTATAC 1 cut(s) 555
BsuRI GGCC 3 cut(s) 596, 721, 917
BtsCI GGATG 2 cut(s) 277, 1189
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 2 cut(s) 391, 1077
Cac8I GCNNGC 3 cut(s) 1020, 1077, 1161
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 462, 595
Csp6I GTAC 2 cut(s) 100, 650
CviAII CATG 4 cut(s) 263, 268, 314, 959
CviQI GTAC 2 cut(s) 100, 650
DpnI GATC 6 cut(s) 31, 214, 372, 444, 533, 957
DpnII GATC 6 cut(s) 29, 212, 370, 442, 531, 955
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 4 cut(s) 105, 465, 610, 918
Eco31I GGTCTC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 1 cut(s) 462
Eco72I CACGTG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 621
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 4 cut(s) 105, 465, 610, 918
ErhI CCWWGG 4 cut(s) 105, 465, 610, 918
FaeI CATG 4 cut(s) 266, 271, 317, 962
FalI AAGNNNNNCTT 2 cut(s) 1027, 1059
FaqI GGGAC 1 cut(s) 556
FatI CATG 4 cut(s) 262, 267, 313, 958
FbaI TGATCA 2 cut(s) 370, 442
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 7 cut(s) 96, 585, 606, 839, 1016, 1023, 1145
FokI GGATG 2 cut(s) 284, 1196
Fsp4HI GCNGC 7 cut(s) 96, 585, 606, 839, 1016, 1023, 1145
FspBI CTAG 2 cut(s) 329, 588
GlaI GCGC 1 cut(s) 130
GluI GCNGC 7 cut(s) 96, 585, 606, 839, 1016, 1023, 1145
GsuI CTGGAG 1 cut(s) 1011
HaeIII GGCC 3 cut(s) 596, 721, 917
HapII CCGG 3 cut(s) 10, 209, 229
HhaI GCGC 1 cut(s) 131
Hin1II CATG 4 cut(s) 266, 271, 317, 962
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 2 cut(s) 569, 879
HpaII CCGG 3 cut(s) 10, 209, 229
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 2 cut(s) 20, 555
Hpy188I TCNGA 9 cut(s) 217, 249, 366, 375, 402, 536, 773, 811, 929
Hpy188III TCNNGA 4 cut(s) 10, 86, 793, 953
Hpy8I GTNNAC 2 cut(s) 20, 555
HpyAV CCTTC 6 cut(s) 61, 244, 416, 584, 586, 703
HpyCH4III ACNGT 2 cut(s) 386, 685
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 9 cut(s) 284, 563, 630, 694, 962, 1025, 1060, 1070, 1163
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 593, 602, 944, 1076, 1150, 1165
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 4 cut(s) 266, 271, 317, 962
HspAI GCGC 1 cut(s) 129
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 370, 442
Kzo9I GATC 6 cut(s) 29, 212, 370, 442, 531, 955
LmnI GCTCC 2 cut(s) 475, 1150
Lsp1109I GCAGC 6 cut(s) 82, 571, 592, 825, 1009, 1156
LweI GCATC 4 cut(s) 550, 889, 934, 1057
MaeI CTAG 2 cut(s) 329, 588
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 755
MalI GATC 6 cut(s) 31, 214, 372, 444, 533, 957
MboI GATC 6 cut(s) 29, 212, 370, 442, 531, 955
MboII GAAGA 3 cut(s) 163, 236, 569
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 2 cut(s) 180, 1081
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 6 cut(s) 418, 621, 823, 1054, 1096, 1193
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 2 cut(s) 578, 873
MmeI TCCRAC 1 cut(s) 528
Mox20I TGGCCA 1 cut(s) 917
MroI TCCGGA 1 cut(s) 9
MscI TGGCCA 1 cut(s) 917
MslI CAYNNNNRTG 2 cut(s) 963, 1065
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 3 cut(s) 10, 209, 229
MspR9I CCNGG 2 cut(s) 210, 229
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 7 cut(s) 290, 593, 602, 944, 1076, 1150, 1165
NciI CCSGG 2 cut(s) 210, 229
NdeII GATC 6 cut(s) 29, 212, 370, 442, 531, 955
NlaIII CATG 4 cut(s) 266, 271, 317, 962
NlaIV GGNNCC 3 cut(s) 471, 597, 817
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 755
NspI RCATGY 1 cut(s) 317
NspV TTCGAA 1 cut(s) 619
Pfl23II CGTACG 1 cut(s) 649
PkrI GCNGC 7 cut(s) 97, 586, 607, 840, 1017, 1024, 1146
PleI GAGTC 2 cut(s) 577, 873
PmaCI CACGTG 1 cut(s) 17
PmlI CACGTG 1 cut(s) 17
PpsI GAGTC 2 cut(s) 577, 873
Ppu21I YACGTR 1 cut(s) 17
PsiI TTATAA 1 cut(s) 854
PspCI CACGTG 1 cut(s) 17
PspLI CGTACG 1 cut(s) 649
PspN4I GGNNCC 3 cut(s) 471, 597, 817
PspPI GGNCC 2 cut(s) 462, 595
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 2 cut(s) 101, 651
RsaNI GTAC 2 cut(s) 100, 650
RseI CAYNNNNRTG 2 cut(s) 963, 1065
SatI GCNGC 7 cut(s) 96, 585, 606, 839, 1016, 1023, 1145
Sau3AI GATC 6 cut(s) 29, 212, 370, 442, 531, 955
Sau96I GGNCC 2 cut(s) 462, 595
SchI GAGTC 2 cut(s) 578, 873
ScrFI CCNGG 2 cut(s) 210, 229
SduI GDGCHC 2 cut(s) 180, 1081
SfaNI GCATC 4 cut(s) 550, 889, 934, 1057
SfcI CTRYAG 2 cut(s) 600, 777
SfuI TTCGAA 1 cut(s) 619
SinI GGWCC 1 cut(s) 462
SmiMI CAYNNNNRTG 2 cut(s) 963, 1065
SmlI CTYRAG 2 cut(s) 86, 297
SmoI CTYRAG 2 cut(s) 86, 297
Sse9I AATT 6 cut(s) 418, 621, 823, 1054, 1096, 1193
SsiI CCGC 4 cut(s) 93, 127, 157, 1016
SspMI CTAG 2 cut(s) 329, 588
StyD4I CCNGG 2 cut(s) 208, 227
StyI CCWWGG 4 cut(s) 105, 465, 610, 918
TaaI ACNGT 2 cut(s) 386, 685
TaiI ACGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 619, 794
TasI AATT 6 cut(s) 418, 621, 823, 1054, 1096, 1193
TauI GCSGC 1 cut(s) 1018
TscAI CASTG 2 cut(s) 391, 1077
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 6 cut(s) 95, 584, 605, 838, 1022, 1144
Tsp45I GTSAC 1 cut(s) 755
TspGWI ACGGA 2 cut(s) 37, 828
TspRI CASTG 2 cut(s) 391, 1077
VpaK11BI GGWCC 1 cut(s) 462
XapI RAATTY 3 cut(s) 621, 1096, 1193
XceI RCATGY 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 2 cut(s) 329, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.