Rroxscaffold_7G00190320

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002752
Physical Location & Seq
Forward (+)
30421374 .. 30424882
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190320.1

Sequence Viewer

Length: 1266 bp
ATGACTCAAGACGTGGAGATGAAAGAGGTCCCGGCCCCTTCCAATTCAGTCTCTTCTACTACACCAACAACTCTCCACCATTTGAAGGAGATTGCTTCGCTTCTTGAGACCGCTGCGTACTCCAAGGAGGTCAGGCGAGTTGTCCGCGCCGTTAGGCTGACGATGGCGAAGAGGCGGAAGCTGAGTGTCTCTGTGCTCTCGGCGTTTCTCAGTTTCGCCTTTTCGCCGGGATCTGAGGCTCACACCCGGTTGTCCTCCTATCTTCCGAAGGACGATGAGCATGACATGGATGTTGATACTGCAACTTCTGCCACTCAAGTTCCCAAGAAACATGCCTCGCCTGAGCTAGAGATTTACTGCTACTTGCTGGTGCTGATTTTTCTGATTGATCAGAAAAAATACAGTGAGGTGAGTGAATATAGTCATCGTGACGTGTGGGTCTTGAACCTTCTTAATCTTCACCGGATTGCAACTTTGCGCCGTGATGAGCTGGGTCAGGAGACACTTCTGAACCTTCTGCTTCGCAATTACCTGTCATATAACCTATATGATCAGGCAGAGAAACTGAGGTCCAAGGCTCCCCGATTTGAGGCTCACTCAAACCAGCAGTTTTGTCGGTATCTCTTTTATCTGGGAAAGATCAGAACCATTCAGTTGGAGTATACTGATGCAAAGGAGTCCCTTCTTCAAGCTGCTAGGAAGGCCCCTGTAGCTGCCCTTGGCTTTCGAATTCAATGCAACAAGTGGGCAGTTATCGTACGCTTGTTGCTTGGAGAAATCCCTGAGAGGACTGTGTTTATGCAAAAAGGAATGGAGAAGGCTTTGAGGCCATATTTTGAACTAACAAATGCTGTGAGGATTGGTGACTTGGAACTATTCAGAACTATAGCTGACAAGTTCTCGAATGTGTTTAGCTCAGACGGAACCCACAATTTGATTATTGGACTCCCCAACCCTGTTGCTGATGCTGAAAGTATTGTGGCCAAGGCAATCCGAGATGGTGCTATTGATGCCACTCTGGATCATGCAAATGGATATATGGTGTCAAAGGAAACTGGAGATATCTACTCTACAAATGAGCCGCAGGCTGCATTTGATACAAGGATTGCTTTCTGCCTCAATTTGCACAATGATGCAGTGCGAGCACTAAGGTTTCCACCAAATTCTCACAAGGAGAAAGAAAGTGCTGAGAAAAGGAGGGAGAGACAGCAGCAGGAGCAAGAGCTTGCAAAGCATATAGCTGAGGAGGATGATGATGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
PCI Rpn3_C

Protein Analysis

421

Amino Acids

47.95

Weight (kDa)

6.52

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PSMD3_N PF25573 23 - 145 3.8e-17 PSMD3-like, N-terminal TPR repeats
TPR_PSMD3_N PF25573 149 - 282 1.2e-61 PSMD3-like, N-terminal TPR repeats
PCI PF01399 319 - 350 5.4e-06 PCI domain
Rpn3_C PF08375 354 - 419 3.3e-27 Proteasome regulatory subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 662
AccII CGCG 1 cut(s) 147
AciI CCGC 4 cut(s) 111, 145, 175, 1082
AclWI GGATC 2 cut(s) 238, 1029
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 3 cut(s) 729, 1162, 1259
AfaI GTAC 2 cut(s) 119, 759
AfiI CCNNNNNNNGG 2 cut(s) 85, 589
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 5 cut(s) 85, 445, 689, 734, 839
AjiI CACGTC 2 cut(s) 13, 433
AluBI AGCT 9 cut(s) 181, 346, 490, 692, 713, 890, 915, 1225, 1241
AluI AGCT 9 cut(s) 181, 346, 490, 692, 713, 890, 915, 1225, 1241
Alw21I GWGCWC 2 cut(s) 198, 1147
Alw26I GTCTC 5 cut(s) 55, 101, 193, 494, 1198
AlwI GGATC 2 cut(s) 238, 1029
AoxI GGCC 4 cut(s) 33, 702, 827, 981
ApeKI GCWGC 5 cut(s) 113, 692, 713, 1088, 1210
ApoI RAATTY 3 cut(s) 729, 1162, 1259
AspLEI GCGC 2 cut(s) 149, 480
AspS9I GGNCC 4 cut(s) 28, 34, 570, 703
AsuC2I CCSGG 3 cut(s) 32, 228, 247
AsuHPI GGTGA 3 cut(s) 421, 452, 875
AsuII TTCGAA 1 cut(s) 727
AvaII GGWCC 2 cut(s) 28, 570
BalI TGGCCA 1 cut(s) 983
Bbv12I GWGCWC 2 cut(s) 198, 1147
BbvCI CCTCAGC 1 cut(s) 1242
BbvI GCAGC 5 cut(s) 100, 679, 700, 1075, 1222
BccI CCATC 2 cut(s) 157, 992
BceAI ACGGC 2 cut(s) 134, 465
BcgI CGANNNNNNTGC 4 cut(s) 596, 630, 717, 751
BclI TGATCA 2 cut(s) 388, 550
BcnI CCSGG 3 cut(s) 32, 228, 247
BcoDI GTCTC 5 cut(s) 55, 101, 193, 494, 1198
BfaI CTAG 2 cut(s) 347, 696
BfmI CTRYAG 2 cut(s) 708, 885
BisI GCNGC 6 cut(s) 114, 693, 714, 1082, 1089, 1211
BlsI GCNGC 6 cut(s) 115, 694, 715, 1083, 1090, 1212
Bme1390I CCNGG 3 cut(s) 32, 228, 247
Bme18I GGWCC 2 cut(s) 28, 570
BmgBI CACGTC 2 cut(s) 13, 433
BmgT120I GGNCC 4 cut(s) 28, 34, 570, 703
BmiI GGNNCC 5 cut(s) 30, 36, 579, 705, 925
BmrFI CCNGG 3 cut(s) 32, 228, 247
BmsI GCATC 4 cut(s) 658, 955, 1000, 1123
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 1077
Bpu10I CCTNAGC 2 cut(s) 342, 1242
Bpu14I TTCGAA 1 cut(s) 727
BpuEI CTTGAG 2 cut(s) 125, 300
BpuMI CCSGG 3 cut(s) 32, 228, 247
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 123, 573, 718, 984
BsaWI WCCGGW 1 cut(s) 462
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 589
Bse1I ACTGG 1 cut(s) 1060
BseDI CCNNGG 4 cut(s) 123, 573, 718, 984
BseGI GGATG 2 cut(s) 295, 1255
BseLI CCNNNNNNNGG 2 cut(s) 85, 589
BseMII CTCAG 9 cut(s) 173, 223, 225, 333, 557, 774, 930, 1179, 1233
BseNI ACTGG 1 cut(s) 1060
BseRI GAGGAG 1 cut(s) 1259
BseXI GCAGC 5 cut(s) 100, 679, 700, 1075, 1222
BseYI CCCAGC 1 cut(s) 490
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 4 cut(s) 35, 704, 829, 983
BsiHKAI GWGCWC 2 cut(s) 198, 1147
BsiSI CCGG 4 cut(s) 32, 227, 247, 463
BsiWI CGTACG 1 cut(s) 757
BslFI GGGAC 2 cut(s) 14, 664
BslI CCNNNNNNNGG 2 cut(s) 85, 589
BsmAI GTCTC 5 cut(s) 55, 101, 193, 494, 1198
BsmFI GGGAC 2 cut(s) 14, 664
BsnI GGCC 4 cut(s) 35, 704, 829, 983
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 198, 1147
Bsp143I GATC 5 cut(s) 230, 388, 550, 639, 1021
BspACI CCGC 4 cut(s) 111, 145, 175, 1082
BspANI GGCC 4 cut(s) 35, 704, 829, 983
BspCNI CTCAG 9 cut(s) 174, 222, 226, 334, 558, 775, 929, 1180, 1234
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 5 cut(s) 30, 36, 579, 705, 925
BspPI GGATC 2 cut(s) 238, 1029
BspT104I TTCGAA 1 cut(s) 727
BspTNI GGTCTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 1060
BssECI CCNNGG 4 cut(s) 123, 573, 718, 984
BssMI GATC 5 cut(s) 230, 388, 550, 639, 1021
BssNAI GTATAC 1 cut(s) 663
BssT1I CCWWGG 4 cut(s) 123, 573, 718, 984
Bst1107I GTATAC 1 cut(s) 663
Bst4CI ACNGT 2 cut(s) 404, 793
Bst6I CTCTTC 2 cut(s) 58, 164
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 727
BstC8I GCNNGC 3 cut(s) 1086, 1143, 1227
BstF5I GGATG 2 cut(s) 295, 1255
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 2 cut(s) 149, 480
BstKTI GATC 5 cut(s) 233, 391, 553, 642, 1024
BstMAI GTCTC 5 cut(s) 55, 101, 193, 494, 1198
BstMBI GATC 5 cut(s) 230, 388, 550, 639, 1021
BstMWI GCNNNNNNNGC 7 cut(s) 308, 701, 710, 1010, 1142, 1216, 1231
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 3 cut(s) 30, 226, 245
BstSFI CTRYAG 2 cut(s) 708, 885
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 100, 679, 700, 1075, 1222
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 655
BstYI RGATCY 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 663
BsuRI GGCC 4 cut(s) 35, 704, 829, 983
BtrI CACGTC 2 cut(s) 13, 433
BtsCI GGATG 2 cut(s) 295, 1255
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 2 cut(s) 409, 1143
Cac8I GCNNGC 3 cut(s) 1086, 1143, 1227
CfoI GCGC 2 cut(s) 149, 480
Cfr13I GGNCC 4 cut(s) 28, 34, 570, 703
Csp6I GTAC 2 cut(s) 118, 758
CviAII CATG 4 cut(s) 281, 286, 332, 1025
CviQI GTAC 2 cut(s) 118, 758
DpnI GATC 5 cut(s) 232, 390, 552, 641, 1023
DpnII GATC 5 cut(s) 230, 388, 550, 639, 1021
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 2 cut(s) 58, 164
EarI CTCTTC 2 cut(s) 58, 164
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 4 cut(s) 123, 573, 718, 984
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 1063
Eco47I GGWCC 2 cut(s) 28, 570
EcoO109I RGGNCCY 2 cut(s) 28, 703
EcoRI GAATTC 1 cut(s) 729
EcoRV GATATC 1 cut(s) 1063
EcoT14I CCWWGG 4 cut(s) 123, 573, 718, 984
ErhI CCWWGG 4 cut(s) 123, 573, 718, 984
FaeI CATG 4 cut(s) 284, 289, 335, 1028
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 2 cut(s) 14, 664
FatI CATG 4 cut(s) 280, 285, 331, 1024
FbaI TGATCA 2 cut(s) 388, 550
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 6 cut(s) 114, 693, 714, 1082, 1089, 1211
FokI GGATG 2 cut(s) 302, 1262
Fsp4HI GCNGC 6 cut(s) 114, 693, 714, 1082, 1089, 1211
FspBI CTAG 2 cut(s) 347, 696
GlaI GCGC 2 cut(s) 148, 479
GluI GCNGC 6 cut(s) 114, 693, 714, 1082, 1089, 1211
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 1 cut(s) 1077
HaeIII GGCC 4 cut(s) 35, 704, 829, 983
HapII CCGG 4 cut(s) 32, 227, 247, 463
HhaI GCGC 2 cut(s) 149, 480
Hin1II CATG 4 cut(s) 284, 289, 335, 1028
Hin6I GCGC 2 cut(s) 147, 478
HinP1I GCGC 2 cut(s) 147, 478
HinfI GANTC 3 cut(s) 4, 677, 945
HpaII CCGG 4 cut(s) 32, 227, 247, 463
HphI GGTGA 3 cut(s) 421, 452, 875
Hpy166II GTNNAC 1 cut(s) 663
Hpy188I TCNGA 9 cut(s) 235, 267, 384, 393, 510, 644, 881, 919, 995
Hpy188III TCNNGA 7 cut(s) 8, 104, 428, 442, 497, 901, 1019
Hpy8I GTNNAC 1 cut(s) 663
HpyAV CCTTC 8 cut(s) 48, 79, 262, 458, 524, 692, 694, 811
HpyCH4III ACNGT 2 cut(s) 404, 793
HpyCH4IV ACGT 2 cut(s) 12, 432
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 701, 710, 1010, 1142, 1216, 1231
HpySE526I ACGT 2 cut(s) 12, 432
Hsp92II CATG 4 cut(s) 284, 289, 335, 1028
HspAI GCGC 2 cut(s) 147, 478
Ksp22I TGATCA 2 cut(s) 388, 550
Kzo9I GATC 5 cut(s) 230, 388, 550, 639, 1021
LmnI GCTCC 2 cut(s) 583, 1216
Lsp1109I GCAGC 5 cut(s) 100, 679, 700, 1075, 1222
LweI GCATC 4 cut(s) 658, 955, 1000, 1123
MaeI CTAG 2 cut(s) 347, 696
MaeII ACGT 2 cut(s) 12, 432
MaeIII GTNAC 2 cut(s) 428, 863
MalI GATC 5 cut(s) 232, 390, 552, 641, 1023
MboI GATC 5 cut(s) 230, 388, 550, 639, 1021
MboII GAAGA 5 cut(s) 45, 181, 254, 449, 677
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 2 cut(s) 198, 1147
MlsI TGGCCA 1 cut(s) 983
MluCI AATT 7 cut(s) 43, 526, 729, 931, 1120, 1162, 1259
MluNI TGGCCA 1 cut(s) 983
MlyI GAGTC 2 cut(s) 686, 939
MmeI TCCRAC 1 cut(s) 636
Mox20I TGGCCA 1 cut(s) 983
MscI TGGCCA 1 cut(s) 983
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 1029, 1131
Msp20I TGGCCA 1 cut(s) 983
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 4 cut(s) 32, 227, 247, 463
MspR9I CCNGG 3 cut(s) 32, 228, 247
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 7 cut(s) 308, 701, 710, 1010, 1142, 1216, 1231
NciI CCSGG 3 cut(s) 32, 228, 247
NdeII GATC 5 cut(s) 230, 388, 550, 639, 1021
NlaIII CATG 4 cut(s) 284, 289, 335, 1028
NlaIV GGNNCC 5 cut(s) 30, 36, 579, 705, 925
NmeAIII GCCGAG 1 cut(s) 179
NmuCI GTSAC 2 cut(s) 428, 863
NspI RCATGY 1 cut(s) 335
NspV TTCGAA 1 cut(s) 727
Pfl23II CGTACG 1 cut(s) 757
PkrI GCNGC 6 cut(s) 115, 694, 715, 1083, 1090, 1212
PleI GAGTC 2 cut(s) 685, 939
PpsI GAGTC 2 cut(s) 685, 939
PpuMI RGGWCCY 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 490
PspLI CGTACG 1 cut(s) 757
PspN4I GGNNCC 5 cut(s) 30, 36, 579, 705, 925
PspPI GGNCC 4 cut(s) 28, 34, 570, 703
PspPPI RGGWCCY 1 cut(s) 28
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 2 cut(s) 119, 759
RsaNI GTAC 2 cut(s) 118, 758
RseI CAYNNNNRTG 2 cut(s) 1029, 1131
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 6 cut(s) 114, 693, 714, 1082, 1089, 1211
Sau3AI GATC 5 cut(s) 230, 388, 550, 639, 1021
Sau96I GGNCC 4 cut(s) 28, 34, 570, 703
SchI GAGTC 2 cut(s) 686, 939
ScrFI CCNGG 3 cut(s) 32, 228, 247
SduI GDGCHC 2 cut(s) 198, 1147
SfaNI GCATC 4 cut(s) 658, 955, 1000, 1123
SfcI CTRYAG 2 cut(s) 708, 885
SfuI TTCGAA 1 cut(s) 727
SinI GGWCC 2 cut(s) 28, 570
SmiMI CAYNNNNRTG 2 cut(s) 1029, 1131
SmlI CTYRAG 3 cut(s) 6, 104, 315
SmoI CTYRAG 3 cut(s) 6, 104, 315
Sse9I AATT 7 cut(s) 43, 526, 729, 931, 1120, 1162, 1259
SsiI CCGC 4 cut(s) 111, 145, 175, 1082
SspMI CTAG 2 cut(s) 347, 696
StyD4I CCNGG 3 cut(s) 30, 226, 245
StyI CCWWGG 4 cut(s) 123, 573, 718, 984
TaaI ACNGT 2 cut(s) 404, 793
TaiI ACGT 2 cut(s) 15, 435
TaqI TCGA 2 cut(s) 727, 902
TasI AATT 7 cut(s) 43, 526, 729, 931, 1120, 1162, 1259
TauI GCSGC 1 cut(s) 1084
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 409, 1143
TseFI GTSAC 2 cut(s) 428, 863
TseI GCWGC 5 cut(s) 113, 692, 713, 1088, 1210
Tsp45I GTSAC 2 cut(s) 428, 863
TspDTI ATGAA 1 cut(s) 35
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 2 cut(s) 409, 1143
VpaK11BI GGWCC 2 cut(s) 28, 570
XapI RAATTY 3 cut(s) 729, 1162, 1259
XceI RCATGY 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 2 cut(s) 347, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.