Rorug03G0164200

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001970
Physical Location & Seq
Forward (+)
13705912 .. 13709397
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0164200.1

Sequence Viewer

Length: 2184 bp
ATGGAGACCATAACCCAGAATGGAAACCACCAGAATGGGATCCAAAACGGTTCCTTGGATGATCTCTGCGTCAAAACAGAGTCCCTCAAAGCAGAGCTCTCTGTTTCGGACCCGCTTAACTGGGGAGCGGCGGCGGAGTCAATGGCAGGCAGCCACCTTGATGAAGTGAAGCGCATGGTGGCCGAGTACAAGAAGCCGGTGGTGAAACTCGGTGGAAAATCCTTGACTATTTCTCAGGTGGCAGCTATAGCCAACCATGACTCTGGTGTCAAGGTTGAACTGGCTGAGTCGGCCAGGGCTGGTGTGAAGGCCAGTAGTGATTGGGTCATGGAGTCCCTGAACAAGGGTACGGACAGCTATGGTGTCACCACCGGGTTCGGTGCAACCTCCCACAGACGAACCAAACAAGGCGCTGCTCTTCAGAAAGAGCTAATTAGATTCTTGAATGCTGGAGTATTTGGCAATGGAACAGAGTCTCACACCCTGCCTCACTCTGCAACAAGAGCAGCCATGCTCGTTAGAATCAACACTCTCCTGCAAGGCTACTCCGGCATAAGATTCGAAATCTTAGAAGCCATCACCAAGTTTCTCAACCACAACATTACTCCCTGCTTGCCTCTCCGCGGCACGATCACCGCCTCCGGAGACCTTGTGCCGCTGTCCTACATCGCCGGACTACTGACTGGCCGCCCCAATTCCAAGGCAGTCGGGCCAAAAGGCGAGACCCTCAATGCCGCTCAGGCTTTCGAACAAGTCGGTATCAGCTCAGGGTTCTTCGAATTGCAGCCCAAAGAAGGACTTGCTCTTGTTAATGGTACTGCTGTTGGCTCTGGCTTGGCCTCCACGGTTCTTTTCGAGACCAACATTTTGGCCTTGCTTTCCGAAATCTTGTCTGCGATTTTCGCTGAAGTAATGCAAGGGAAGCCCGAGTTTACAGACCACTTGACACACAAGTTAAAGCACCACCCTGGTCAAATTGAGGCTGCTGCAATTATGGAATACATTTTGGATGGCAGTGATTACGTTAAAGCTGCTAAGAAGCTACATGAGCAGGACCCTCTTCAGAAGCCTAAACAAGACCGTTACGCTCTGCGGACCTCACCACAATGGCTCGGTCCACAAATTGAAGTGATTCGGTTTTCAACTAAATCTATCGAGAGGGAGATTAATTCTGTAAATGACAACCCTTTGATTGATGTTTCAAGGAACAAGGCATTGCATGGTGGCAACTTCCAGGGTACGCCAATTGGAGTGTCCATGGATAACACCCGTTTGGCTATTGCATCCATTGGGAAGCTCATGTTTGCTCAGTTTTCGGAACTCGTCAATGACTTTTACAACAACGGCTTGCCGTCCAATTTGTCCGGTGGGAGGAACCCAAGTTTGGATTACGGCTTTAAGGGAGCTGAGATTGCCATGGCATCTTATTGTTCTGAGCTTCAGTTTCTCGCAAATCCAGTGACTAACCATGTCCAGAGTGCTGAGCAGCACAACCAGGATGTCAACTCTTTGGGGTTGATTTCGTCACGAAAAACCGCAGAAGCTGTTGACATACTGAAGCTCATGTCTTCCACATTTATGGTAGCGCTTTGCCAAGCTATTGACTTGAGGCATTTGGAGGAGAACTTGAAGAGCACGGTTAAGAACACTGTGAGTCAATTGGCCAAGAGGCTTTTGACTACCGGGGTTAATGGAGAGCTTCACCCTTCAAGATTCTGCGAGAAGGATTTGCTTATGGTTGTTGAAAGGGAGTACCTTTTCGCCTACATTGATGATCCTTGCAGCGCAACATATCCCTTAATGCAGAGGCTAAGGCAAGTGCTTGTTGAACACGCGTTGACAAATGGTGAGAATGAGAAGAATGCAAACACTTCAATTTTCCAAAAGATTTCGGCATTTGAGGAAGAGCTGAAGACCATTTTGCCTAAAGAGGTTGAGAGCGCTAGGGCCGCATACGAGAGCGGGAATGCTGCGATTCCAAACAGAATCATCGAGTGCAGGTCTTATCCTTTGTACAAATTTGTGAGGGAGGAGTTGGGGGGAGAGTTCCTGACTGGCGAAAAGTTCAGGTCCCCCGGGGAGGAATGTGACAAGGTGTTCACAGCTATGTGCCAGGGGAAAATCATTGATCCGATTCTCAATTGCCTCAGTGGTTGGAACGGTGAACCTCTTCCGATCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001678 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010182 GO:0010243 GO:0010255 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0019941 GO:0022624 GO:0023052 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032991 GO:0033500 GO:0033554 GO:0034284 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042592 GO:0042593 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055082 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700 GO:1901701 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

727

Amino Acids

79.2

Weight (kDa)

6.03

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_aromatic PF00221 75 - 550 2.2e-154 Aromatic amino acid lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 1989
AccBSI CCGCTC 3 cut(s) 128, 737, 1962
AccII CGCG 2 cut(s) 624, 1835
AccIII TCCGGA 1 cut(s) 641
AclWI GGATC 4 cut(s) 34, 47, 1769, 2123
AcoI YGGCCR 4 cut(s) 180, 291, 685, 1662
AcsI RAATTY 1 cut(s) 2018
AcuI CTGAAG 6 cut(s) 404, 927, 1046, 1424, 1577, 1931
AfaI GTAC 6 cut(s) 188, 349, 817, 1240, 1754, 2015
AfeI AGCGCT 2 cut(s) 1587, 1942
AfiI CCNNNNNNNGG 6 cut(s) 343, 623, 794, 1371, 1384, 2080
AflIII ACRYGT 1 cut(s) 1833
AjnI CCWGG 5 cut(s) 293, 967, 1233, 1494, 2112
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
Alw21I GWGCWC 2 cut(s) 99, 1637
Alw26I GTCTC 4 cut(s) 480, 639, 716, 851
AlwI GGATC 4 cut(s) 34, 47, 1769, 2123
AlwNI CAGNNNCTG 1 cut(s) 1544
Ama87I CYCGRG 2 cut(s) 926, 2075
Aor13HI TCCGGA 1 cut(s) 641
Aor51HI AGCGCT 2 cut(s) 1587, 1942
AoxI GGCC 9 cut(s) 180, 291, 309, 685, 710, 837, 870, 1662, 1947
ApoI RAATTY 1 cut(s) 2018
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AseI ATTAAT 1 cut(s) 1167
Asp700I GAANNNNTTC 2 cut(s) 1131, 2169
AspLEI GCGC 5 cut(s) 174, 413, 1588, 1787, 1943
AspS9I GGNCC 7 cut(s) 109, 710, 1054, 1095, 1115, 1947, 2070
AsuC2I CCSGG 4 cut(s) 373, 1684, 2076, 2077
AsuHPI GGTGA 8 cut(s) 214, 358, 571, 625, 1092, 1694, 1859, 2174
AsuII TTCGAA 3 cut(s) 561, 747, 777
AvaI CYCGRG 2 cut(s) 926, 2075
AvaII GGWCC 5 cut(s) 109, 1054, 1095, 1115, 2070
BaeI ACNNNNGTAYC 2 cut(s) 807, 840
BalI TGGCCA 1 cut(s) 1664
BamHI GGATCC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 1560, 1919
Bbv12I GWGCWC 2 cut(s) 99, 1637
BccI CCATC 2 cut(s) 584, 1004
BceAI ACGGC 3 cut(s) 1336, 1360, 1408
BcgI CGANNNNNNTGC 2 cut(s) 541, 575
BciT130I CCWGG 5 cut(s) 295, 969, 1235, 1496, 2114
BcnI CCSGG 4 cut(s) 373, 1684, 2076, 2077
BcoDI GTCTC 4 cut(s) 480, 639, 716, 851
BfaI CTAG 2 cut(s) 1944, 2182
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 3 cut(s) 414, 1589, 1944
BfuAI ACCTGC 1 cut(s) 1989
BglI GCCNNNNNGGC 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 1482
Bme1390I CCNGG 9 cut(s) 295, 373, 969, 1235, 1496, 1684, 2076, 2077, 2114
Bme18I GGWCC 5 cut(s) 109, 1054, 1095, 1115, 2070
BmeT110I CYCGRG 2 cut(s) 926, 2075
BmgT120I GGNCC 7 cut(s) 109, 710, 1054, 1095, 1115, 1947, 2070
BmiI GGNNCC 6 cut(s) 41, 52, 111, 1056, 1376, 2072
BmrFI CCNGG 9 cut(s) 295, 373, 969, 1235, 1496, 1684, 2076, 2077, 2114
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 2 cut(s) 1292, 1430
BmuI ACTGGG 1 cut(s) 130
BpiI GAAGAC 2 cut(s) 1560, 1919
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 3 cut(s) 738, 766, 1811
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 3 cut(s) 561, 747, 777
BpuEI CTTGAG 1 cut(s) 1627
BpuMI CCSGG 4 cut(s) 373, 1684, 2076, 2077
BsaI GGTCTC 3 cut(s) 639, 716, 851
BsaWI WCCGGW 2 cut(s) 641, 1364
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 6 cut(s) 343, 623, 794, 1371, 1384, 2080
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 6 cut(s) 125, 285, 312, 688, 1457, 2059
Bse3DI GCAATG 2 cut(s) 469, 1214
BseAI TCCGGA 1 cut(s) 641
BseBI CCWGG 5 cut(s) 295, 969, 1235, 1496, 2114
BseGI GGATG 4 cut(s) 64, 1015, 1283, 1504
BseLI CCNNNNNNNGG 6 cut(s) 343, 623, 794, 1371, 1384, 2080
BseMI GCAATG 2 cut(s) 469, 1214
BseMII CTCAG 9 cut(s) 248, 276, 752, 780, 1322, 1398, 1425, 1473, 2161
BseNI ACTGG 6 cut(s) 125, 285, 312, 688, 1457, 2059
BseRI GAGGAG 2 cut(s) 1634, 2045
BsgI GTGCAG 1 cut(s) 2017
Bsh1236I CGCG 2 cut(s) 624, 1835
BshFI GGCC 9 cut(s) 182, 293, 311, 687, 712, 839, 872, 1664, 1949
BsiHKAI GWGCWC 2 cut(s) 99, 1637
BsiHKCI CYCGRG 2 cut(s) 926, 2075
BsiSI CCGG 8 cut(s) 197, 372, 549, 642, 672, 1365, 1683, 2076
BslFI GGGAC 3 cut(s) 67, 319, 2056
BslI CCNNNNNNNGG 6 cut(s) 343, 623, 794, 1371, 1384, 2080
BsmAI GTCTC 4 cut(s) 480, 639, 716, 851
BsmFI GGGAC 3 cut(s) 67, 319, 2056
BsmI GAATGC 3 cut(s) 451, 1867, 1972
BsnI GGCC 9 cut(s) 182, 293, 311, 687, 712, 839, 872, 1664, 1949
Bso31I GGTCTC 3 cut(s) 639, 716, 851
BsoBI CYCGRG 2 cut(s) 926, 2075
Bsp119I TTCGAA 3 cut(s) 561, 747, 777
Bsp1286I GDGCHC 2 cut(s) 99, 1637
Bsp13I TCCGGA 1 cut(s) 641
Bsp1407I TGTACA 1 cut(s) 2013
Bsp143I GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
Bsp1720I GCTNAGC 1 cut(s) 1482
Bsp19I CCATGG 2 cut(s) 1257, 1416
BspANI GGCC 9 cut(s) 182, 293, 311, 687, 712, 839, 872, 1664, 1949
BspCNI CTCAG 9 cut(s) 247, 277, 751, 779, 1321, 1399, 1426, 1474, 2160
BspEI TCCGGA 1 cut(s) 641
BspFNI CGCG 2 cut(s) 624, 1835
BspLI GGNNCC 6 cut(s) 41, 52, 111, 1056, 1376, 2072
BspMI ACCTGC 1 cut(s) 1989
BspPI GGATC 4 cut(s) 34, 47, 1769, 2123
BspQI GCTCTTC 3 cut(s) 423, 1625, 1899
BspT104I TTCGAA 3 cut(s) 561, 747, 777
BspTNI GGTCTC 3 cut(s) 639, 716, 851
BsrBI CCGCTC 3 cut(s) 128, 737, 1962
BsrDI GCAATG 2 cut(s) 469, 1214
BsrFI RCCGGY 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 2013
BsrI ACTGG 6 cut(s) 125, 285, 312, 688, 1457, 2059
BssAI RCCGGY 1 cut(s) 196
BssMI GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
BssT1I CCWWGG 4 cut(s) 54, 699, 1257, 1416
Bst2UI CCWGG 5 cut(s) 295, 969, 1235, 1496, 2114
Bst4CI ACNGT 6 cut(s) 50, 847, 1082, 1639, 1651, 2162
Bst6I CTCTTC 5 cut(s) 423, 1065, 1625, 1899, 2175
BstAUI TGTACA 1 cut(s) 2013
BstBI TTCGAA 3 cut(s) 561, 747, 777
BstC8I GCNNGC 3 cut(s) 148, 614, 1349
BstDSI CCRYGG 4 cut(s) 622, 843, 1257, 1416
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 4 cut(s) 64, 1015, 1283, 1504
BstFNI CGCG 2 cut(s) 624, 1835
BstH2I RGCGCY 3 cut(s) 414, 1589, 1944
BstHHI GCGC 5 cut(s) 174, 413, 1588, 1787, 1943
BstKTI GATC 6 cut(s) 42, 64, 633, 1777, 2131, 2178
BstMAI GTCTC 4 cut(s) 480, 639, 716, 851
BstMBI GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
BstNI CCWGG 5 cut(s) 295, 969, 1235, 1496, 2114
BstSCI CCNGG 9 cut(s) 293, 371, 967, 1233, 1494, 1682, 2074, 2075, 2112
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 2 cut(s) 624, 1835
BstV2I GAAGAC 2 cut(s) 1560, 1919
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 4 cut(s) 35, 263, 868, 1579
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 9 cut(s) 182, 293, 311, 687, 712, 839, 872, 1664, 1949
BtgI CCRYGG 4 cut(s) 622, 843, 1257, 1416
BtgZI GCGATG 1 cut(s) 652
BtsCI GGATG 4 cut(s) 64, 1015, 1283, 1504
BtsI GCAGTG 1 cut(s) 1021
BtsIMutI CAGTG 4 cut(s) 1021, 1464, 1647, 2155
BveI ACCTGC 1 cut(s) 1989
Cac8I GCNNGC 3 cut(s) 148, 614, 1349
CaiI CAGNNNCTG 1 cut(s) 1544
CfoI GCGC 5 cut(s) 174, 413, 1588, 1787, 1943
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 7 cut(s) 109, 710, 1054, 1095, 1115, 1947, 2070
Cfr42I CCGCGG 1 cut(s) 625
Cfr9I CCCGGG 1 cut(s) 2075
CseI GACGC 1 cut(s) 58
Csp6I GTAC 6 cut(s) 187, 348, 816, 1239, 1753, 2014
CviQI GTAC 6 cut(s) 187, 348, 816, 1239, 1753, 2014
DpnI GATC 6 cut(s) 41, 63, 632, 1776, 2130, 2177
DpnII GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 4 cut(s) 180, 291, 685, 1662
Eam1104I CTCTTC 5 cut(s) 423, 1065, 1625, 1899, 2175
EarI CTCTTC 5 cut(s) 423, 1065, 1625, 1899, 2175
EciI GGCGGA 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 4 cut(s) 54, 699, 1257, 1416
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 3 cut(s) 639, 716, 851
Eco47I GGWCC 5 cut(s) 109, 1054, 1095, 1115, 2070
Eco47III AGCGCT 2 cut(s) 1587, 1942
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 6 cut(s) 404, 927, 1046, 1424, 1577, 1931
Eco88I CYCGRG 2 cut(s) 926, 2075
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 1054, 2070
EcoRII CCWGG 5 cut(s) 293, 967, 1233, 1494, 2112
EcoT14I CCWWGG 4 cut(s) 54, 699, 1257, 1416
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 4 cut(s) 54, 699, 1257, 1416
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 3 cut(s) 67, 319, 2056
FauI CCCGC 2 cut(s) 120, 1955
FokI GGATG 4 cut(s) 71, 1022, 1270, 1511
FriOI GRGCYC 1 cut(s) 99
FspBI CTAG 2 cut(s) 1944, 2182
GlaI GCGC 5 cut(s) 173, 412, 1587, 1786, 1942
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 3 cut(s) 414, 1589, 1944
HaeIII GGCC 9 cut(s) 182, 293, 311, 687, 712, 839, 872, 1664, 1949
HapII CCGG 8 cut(s) 197, 372, 549, 642, 672, 1365, 1683, 2076
HgaI GACGC 1 cut(s) 58
HhaI GCGC 5 cut(s) 174, 413, 1588, 1787, 1943
Hin6I GCGC 5 cut(s) 172, 411, 1586, 1785, 1941
HinP1I GCGC 5 cut(s) 172, 411, 1586, 1785, 1941
HincII GTYRAC 3 cut(s) 1504, 1549, 1839
HindII GTYRAC 3 cut(s) 1504, 1549, 1839
HpaII CCGG 8 cut(s) 197, 372, 549, 642, 672, 1365, 1683, 2076
HphI GGTGA 8 cut(s) 214, 358, 571, 625, 1092, 1694, 1859, 2174
Hpy166II GTNNAC 7 cut(s) 933, 1118, 1504, 1549, 1839, 2100, 2165
Hpy188I TCNGA 8 cut(s) 109, 423, 883, 1065, 1318, 1435, 2133, 2175
Hpy188III TCNNGA 8 cut(s) 442, 642, 856, 1156, 1474, 1527, 1710, 2050
Hpy8I GTNNAC 7 cut(s) 933, 1118, 1504, 1549, 1839, 2100, 2165
HpyAV CCTTC 4 cut(s) 301, 788, 1716, 1717
HpyCH4III ACNGT 6 cut(s) 50, 847, 1082, 1639, 1651, 2162
HpyCH4IV ACGT 1 cut(s) 1023
HpySE526I ACGT 1 cut(s) 1023
HspAI GCGC 5 cut(s) 172, 411, 1586, 1785, 1941
Kpn2I TCCGGA 1 cut(s) 641
KspI CCGCGG 1 cut(s) 625
Kzo9I GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
LguI GCTCTTC 3 cut(s) 423, 1625, 1899
LmnI GCTCC 2 cut(s) 125, 1403
LweI GCATC 2 cut(s) 1292, 1430
MaeI CTAG 2 cut(s) 1944, 2182
MaeII ACGT 1 cut(s) 1023
MaeIII GTNAC 5 cut(s) 364, 1082, 1459, 1524, 2087
MalI GATC 6 cut(s) 41, 63, 632, 1776, 2130, 2177
MbiI CCGCTC 3 cut(s) 128, 737, 1962
MboI GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
MboII GAAGA 9 cut(s) 410, 766, 1052, 1560, 1642, 1870, 1916, 1924, 2162
MfeI CAATTG 3 cut(s) 1245, 1658, 2140
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 2 cut(s) 99, 1637
MlsI TGGCCA 1 cut(s) 1664
MluI ACGCGT 1 cut(s) 1833
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 7 cut(s) 89, 146, 254, 296, 341, 482, 1663
MmeI TCCRAC 1 cut(s) 2135
Mox20I TGGCCA 1 cut(s) 1664
MroI TCCGGA 1 cut(s) 641
MroXI GAANNNNTTC 2 cut(s) 1131, 2169
MscI TGGCCA 1 cut(s) 1664
MseI TTAA 9 cut(s) 117, 810, 956, 1026, 1167, 1398, 1641, 1689, 1799
MslI CAYNNNNRTG 5 cut(s) 33, 159, 1105, 1577, 2105
Msp20I TGGCCA 1 cut(s) 1664
MspA1I CMGCKG 2 cut(s) 624, 658
MspI CCGG 8 cut(s) 197, 372, 549, 642, 672, 1365, 1683, 2076
MspR9I CCNGG 9 cut(s) 295, 373, 969, 1235, 1496, 1684, 2076, 2077, 2114
MunI CAATTG 3 cut(s) 1245, 1658, 2140
Mva1269I GAATGC 3 cut(s) 451, 1867, 1972
MvaI CCWGG 5 cut(s) 295, 969, 1235, 1496, 2114
MvnI CGCG 2 cut(s) 624, 1835
NciI CCSGG 4 cut(s) 373, 1684, 2076, 2077
NcoI CCATGG 2 cut(s) 1257, 1416
NdeII GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
NlaIV GGNNCC 6 cut(s) 41, 52, 111, 1056, 1376, 2072
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 4 cut(s) 364, 1459, 1524, 2087
NspV TTCGAA 3 cut(s) 561, 747, 777
PciSI GCTCTTC 3 cut(s) 423, 1625, 1899
PctI GAATGC 3 cut(s) 451, 1867, 1972
PdmI GAANNNNTTC 2 cut(s) 1131, 2169
PfeI GAWTC 8 cut(s) 438, 522, 558, 1132, 1713, 1975, 1986, 2134
PleI GAGTC 7 cut(s) 88, 145, 254, 295, 340, 481, 1662
PpsI GAGTC 7 cut(s) 88, 145, 254, 295, 340, 481, 1662
PpuMI RGGWCCY 2 cut(s) 1054, 2070
PshBI ATTAAT 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 2 cut(s) 1054, 2070
Psp6I CCWGG 5 cut(s) 293, 967, 1233, 1494, 2112
PspGI CCWGG 5 cut(s) 293, 967, 1233, 1494, 2112
PspN4I GGNNCC 6 cut(s) 41, 52, 111, 1056, 1376, 2072
PspPI GGNCC 7 cut(s) 109, 710, 1054, 1095, 1115, 1947, 2070
PspPPI RGGWCCY 2 cut(s) 1054, 2070
PstNI CAGNNNCTG 1 cut(s) 1544
PsuI RGATCY 1 cut(s) 39
RsaI GTAC 6 cut(s) 188, 349, 817, 1240, 1754, 2015
RsaNI GTAC 6 cut(s) 187, 348, 816, 1239, 1753, 2014
RseI CAYNNNNRTG 5 cut(s) 33, 159, 1105, 1577, 2105
SacI GAGCTC 1 cut(s) 99
SacII CCGCGG 1 cut(s) 625
SapI GCTCTTC 3 cut(s) 423, 1625, 1899
SaqAI TTAA 9 cut(s) 117, 810, 956, 1026, 1167, 1398, 1641, 1689, 1799
Sau3AI GATC 6 cut(s) 39, 61, 630, 1774, 2128, 2175
Sau96I GGNCC 7 cut(s) 109, 710, 1054, 1095, 1115, 1947, 2070
SchI GAGTC 7 cut(s) 89, 146, 254, 296, 341, 482, 1663
ScrFI CCNGG 9 cut(s) 295, 373, 969, 1235, 1496, 1684, 2076, 2077, 2114
SduI GDGCHC 2 cut(s) 99, 1637
SfaNI GCATC 2 cut(s) 1292, 1430
SfcI CTRYAG 1 cut(s) 246
Sfr303I CCGCGG 1 cut(s) 625
SfuI TTCGAA 3 cut(s) 561, 747, 777
SgrBI CCGCGG 1 cut(s) 625
SinI GGWCC 5 cut(s) 109, 1054, 1095, 1115, 2070
SmaI CCCGGG 1 cut(s) 2077
SmiMI CAYNNNNRTG 5 cut(s) 33, 159, 1105, 1577, 2105
SmlI CTYRAG 1 cut(s) 1606
SmoI CTYRAG 1 cut(s) 1606
SspMI CTAG 2 cut(s) 1944, 2182
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 9 cut(s) 293, 371, 967, 1233, 1494, 1682, 2074, 2075, 2112
StyI CCWWGG 4 cut(s) 54, 699, 1257, 1416
TaaI ACNGT 6 cut(s) 50, 847, 1082, 1639, 1651, 2162
TaiI ACGT 1 cut(s) 1026
TaqI TCGA 6 cut(s) 561, 747, 777, 855, 1155, 1992
TaqII GACCGA 1 cut(s) 1103
TatI WGTACW 2 cut(s) 186, 2013
TauI GCSGC 7 cut(s) 131, 134, 627, 658, 690, 737, 1952
TfiI GAWTC 8 cut(s) 438, 522, 558, 1132, 1713, 1975, 1986, 2134
Tru1I TTAA 9 cut(s) 117, 810, 956, 1026, 1167, 1398, 1641, 1689, 1799
Tru9I TTAA 9 cut(s) 117, 810, 956, 1026, 1167, 1398, 1641, 1689, 1799
TscAI CASTG 4 cut(s) 1021, 1464, 1654, 2155
TseFI GTSAC 4 cut(s) 364, 1459, 1524, 2087
Tsp45I GTSAC 4 cut(s) 364, 1459, 1524, 2087
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 365
TspMI CCCGGG 1 cut(s) 2075
TspRI CASTG 4 cut(s) 1021, 1464, 1654, 2155
VpaK11BI GGWCC 5 cut(s) 109, 1054, 1095, 1115, 2070
VspI ATTAAT 1 cut(s) 1167
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 1 cut(s) 2018
XmaI CCCGGG 1 cut(s) 2075
XmnI GAANNNNTTC 2 cut(s) 1131, 2169
XspI CTAG 2 cut(s) 1944, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.