Rorug03G0164300

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001970
Physical Location & Seq
Forward (+)
13749656 .. 13753286
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0164300.1

Sequence Viewer

Length: 1131 bp
ATGGCTTCCCAGCTATTGAGGCGCGTAATCGGAAGCCGCTCCACCCACTTTCTCTCCGGCGCCGCGAATCCCTCCCCGACCTTGCTCTCATCGTCGTCTCCGGCAGCTAGGGTTTTCTCATCTACTTCCACTCCGATCACTGCCACCCTCTTCCCCGGCGACGGTATCGGCCCCGAGATCGCCGAGTCCGTCAAACAGATATTCAGAGAAGCTGAAGTCCCAATTGAATGGGAAGAACACTATGTTGGGGAGCAAATAGATCCCAGGACTCAGAGTTTTCTGACATGGGAAAGTTTAGAGTCGGTACGAAGGAATAAGGTGGGCTTGAAAGGGCCGATGGCCACACCAATTGGGAAGGGTCATCGTTCATTGAACCTTACACTGAGGAAAGAACTGAATTTGTTTGCAAATGTCAGACCTTGCTACAGCCTTCCTGGCTATAAGACTCGATATGATGATGTAGATCTTGTCACTATCCGTGAGAATACGGAGGGGGAATACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTTGTTGAGAGCCTCAAGATCATTACCCGTCAAGCTAGCTTGAGGGTGGCTGAGTATGCTTTTCATTATGCCAAGGCCCATGGAAGAGAAAGAGTGTCTGCAATACACAAAGCAAATATAATGCAGAAAACCGACGGTCTTTTTCTCAAGTGTTGCCGCGAAGTTGCAGAGAAGTACCCTGATATAAAGTATGAGGAAGTTGTCATTGATAATTGCTGTATGATGCTTGTGAAGAATCCAGCACTTTTTGATGTGCTAGTGATGCCTAACCTCTATGGTGATATTATTAGTGACCTTTGCGCTGGGTTGATAGGGGGTTTGGGCTTAACACCAAGCTGCAATATTGGTGAGGGAGGTATTGCCCTTGCTGAAGCTGTACATGGATCGGCACCTGATATTGCTGGAAAGAATTTGGCAAATCCAACTGCTCTGCTTTTGAGTGCTGTTACCATGCTTCGCCATTTGGAGCTCCATGATAAAGCTGATCGGATCCAGGATGCGATCCTGAAGACAATTGCAGAGGGCAAGTACCGTACTGCTGACCTTGGTGGCAAATCAACAACTACTGACTTTACACAAGCGATTTGTGATCATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001678 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010182 GO:0010243 GO:0010255 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0019941 GO:0022624 GO:0023052 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032991 GO:0033500 GO:0033554 GO:0034284 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042592 GO:0042593 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055082 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700 GO:1901701 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

376

Amino Acids

41.11

Weight (kDa)

6.43

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 49 - 372 8.7e-109 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 59, 919
AccBSI CCGCTC 1 cut(s) 39
AccII CGCG 3 cut(s) 24, 65, 690
AciI CCGC 3 cut(s) 37, 63, 688
AclWI GGATC 5 cut(s) 254, 922, 1015, 1027, 1028
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 2 cut(s) 397, 940
AcuI CTGAAG 3 cut(s) 234, 921, 1058
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 5 cut(s) 306, 707, 909, 1061, 1066
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 4 cut(s) 227, 328, 373, 512
AjnI CCWGG 3 cut(s) 263, 433, 1023
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AluBI AGCT 9 cut(s) 13, 107, 212, 566, 570, 867, 905, 1000, 1013
AluI AGCT 9 cut(s) 13, 107, 212, 566, 570, 867, 905, 1000, 1013
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 5 cut(s) 254, 922, 1015, 1027, 1028
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 4 cut(s) 169, 332, 339, 606
ApeKI GCWGC 2 cut(s) 104, 867
ApoI RAATTY 2 cut(s) 397, 940
AspLEI GCGC 3 cut(s) 24, 62, 833
AspS9I GGNCC 3 cut(s) 170, 332, 607
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 535, 821, 890
AsuNHI GCTAGC 1 cut(s) 566
AvaI CYCGRG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 341
BamHI GGATCC 1 cut(s) 1020
BanI GGYRCC 2 cut(s) 59, 919
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 1 cut(s) 1046
Bbv12I GWGCWC 1 cut(s) 1002
BbvI GCAGC 2 cut(s) 116, 854
BccI CCATC 1 cut(s) 331
BciT130I CCWGG 3 cut(s) 265, 435, 1025
BclI TGATCA 1 cut(s) 1120
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 3 cut(s) 108, 567, 788
BfmI CTRYAG 1 cut(s) 424
BfoI RGCGCY 1 cut(s) 63
BglI GCCNNNNNGGC 1 cut(s) 435
BglII AGATCT 1 cut(s) 463
BisI GCNGC 5 cut(s) 37, 63, 105, 688, 868
BlsI GCNGC 5 cut(s) 38, 64, 106, 689, 869
Bme1390I CCNGG 4 cut(s) 156, 265, 435, 1025
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 3 cut(s) 170, 332, 607
BmiI GGNNCC 4 cut(s) 61, 172, 921, 1022
BmrFI CCNGG 4 cut(s) 156, 265, 435, 1025
BmsI GCATC 3 cut(s) 744, 783, 1018
BmtI GCTAGC 1 cut(s) 570
BpiI GAAGAC 1 cut(s) 1046
BpuEI CTTGAG 3 cut(s) 530, 592, 662
BpuMI CCSGG 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 5 cut(s) 154, 263, 603, 610, 1075
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 115, 145
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 3 cut(s) 265, 435, 1025
BseDI CCNNGG 5 cut(s) 154, 263, 603, 610, 1075
BseGI GGATG 1 cut(s) 1033
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 3 cut(s) 284, 374, 573
BseXI GCAGC 2 cut(s) 116, 854
BseYI CCCAGC 2 cut(s) 9, 833
Bsh1236I CGCG 3 cut(s) 24, 65, 690
BshFI GGCC 4 cut(s) 171, 334, 341, 608
BshNI GGYRCC 2 cut(s) 59, 919
BsiHKAI GWGCWC 1 cut(s) 1002
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 57, 101, 156
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 102
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 4 cut(s) 171, 334, 341, 608
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp1407I TGTACA 1 cut(s) 907
Bsp19I CCATGG 1 cut(s) 610
BspACI CCGC 3 cut(s) 37, 63, 688
BspANI GGCC 4 cut(s) 171, 334, 341, 608
BspCNI CTCAG 3 cut(s) 283, 375, 574
BspFNI CGCG 3 cut(s) 24, 65, 690
BspLI GGNNCC 4 cut(s) 61, 172, 921, 1022
BspOI GCTAGC 1 cut(s) 570
BspPI GGATC 5 cut(s) 254, 922, 1015, 1027, 1028
BspT107I GGYRCC 2 cut(s) 59, 919
BsrBI CCGCTC 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 907
BssECI CCNNGG 5 cut(s) 154, 263, 603, 610, 1075
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 3 cut(s) 603, 610, 1075
Bst2UI CCWGG 3 cut(s) 265, 435, 1025
Bst4CI ACNGT 4 cut(s) 164, 503, 668, 1064
Bst6I CTCTTC 2 cut(s) 155, 610
BstACI GRCGYC 1 cut(s) 60
BstAUI TGTACA 1 cut(s) 907
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 3 cut(s) 270, 383, 582
BstDSI CCRYGG 1 cut(s) 610
BstF5I GGATG 1 cut(s) 1033
BstFNI CGCG 3 cut(s) 24, 65, 690
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 3 cut(s) 24, 62, 833
BstMAI GTCTC 1 cut(s) 102
BstMWI GCNNNNNNNGC 4 cut(s) 19, 435, 587, 793
BstNI CCWGG 3 cut(s) 265, 435, 1025
BstSCI CCNGG 4 cut(s) 154, 263, 433, 1023
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 3 cut(s) 24, 65, 690
BstV1I GCAGC 2 cut(s) 116, 854
BstV2I GAAGAC 1 cut(s) 1046
BstX2I RGATCY 3 cut(s) 259, 463, 1020
BstXI CCANNNNNNTGG 1 cut(s) 228
BstYI RGATCY 3 cut(s) 259, 463, 1020
BsuRI GGCC 4 cut(s) 171, 334, 341, 608
BtgI CCRYGG 1 cut(s) 610
BtsCI GGATG 1 cut(s) 1033
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 138, 380, 508
Cac8I GCNNGC 1 cut(s) 568
CfoI GCGC 3 cut(s) 24, 62, 833
Cfr13I GGNCC 3 cut(s) 170, 332, 607
Csp6I GTAC 5 cut(s) 305, 706, 908, 1060, 1065
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 5 cut(s) 285, 611, 911, 982, 1004
CviQI GTAC 5 cut(s) 305, 706, 908, 1060, 1065
DdeI CTNAG 3 cut(s) 270, 383, 582
DinI GGCGCC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 155, 610
EarI CTCTTC 2 cut(s) 155, 610
Ecl136II GAGCTC 1 cut(s) 1000
Eco130I CCWWGG 3 cut(s) 603, 610, 1075
Eco24I GRGCYC 1 cut(s) 1002
Eco53kI GAGCTC 1 cut(s) 1000
Eco57I CTGAAG 3 cut(s) 234, 921, 1058
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 263, 433, 1023
EcoT14I CCWWGG 3 cut(s) 603, 610, 1075
EcoT38I GRGCYC 1 cut(s) 1002
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 603, 610, 1075
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 5 cut(s) 288, 614, 914, 985, 1007
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 1 cut(s) 203
FatI CATG 5 cut(s) 284, 610, 910, 981, 1003
FbaI TGATCA 1 cut(s) 1120
Fnu4HI GCNGC 5 cut(s) 37, 63, 105, 688, 868
FokI GGATG 1 cut(s) 1040
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 5 cut(s) 37, 63, 105, 688, 868
FspBI CTAG 3 cut(s) 108, 567, 788
GlaI GCGC 3 cut(s) 23, 61, 832
GluI GCNGC 5 cut(s) 37, 63, 105, 688, 868
GsaI CCCAGC 2 cut(s) 13, 837
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 4 cut(s) 171, 334, 341, 608
HapII CCGG 3 cut(s) 57, 101, 156
HhaI GCGC 3 cut(s) 24, 62, 833
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 5 cut(s) 288, 614, 914, 985, 1007
Hin6I GCGC 3 cut(s) 22, 60, 831
HinP1I GCGC 3 cut(s) 22, 60, 831
HinfI GANTC 6 cut(s) 67, 185, 268, 299, 445, 766
HpaII CCGG 3 cut(s) 57, 101, 156
HphI GGTGA 3 cut(s) 535, 821, 890
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 8 cut(s) 32, 135, 206, 273, 282, 416, 1020, 1130
Hpy188III TCNNGA 2 cut(s) 547, 1036
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 3 cut(s) 97, 164, 668
HpyAV CCTTC 3 cut(s) 303, 349, 440
HpyCH4III ACNGT 4 cut(s) 164, 503, 668, 1064
HpyCH4V TGCA 6 cut(s) 407, 632, 655, 698, 870, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 19, 435, 587, 793
HpyF3I CTNAG 3 cut(s) 270, 383, 582
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 5 cut(s) 288, 614, 914, 985, 1007
HspAI GCGC 3 cut(s) 22, 60, 831
KasI GGCGCC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 1120
LmnI GCTCC 4 cut(s) 44, 250, 997, 1005
Lsp1109I GCAGC 2 cut(s) 116, 854
LweI GCATC 3 cut(s) 744, 783, 1018
MaeI CTAG 3 cut(s) 108, 567, 788
MaeIII GTNAC 3 cut(s) 469, 821, 976
MbiI CCGCTC 1 cut(s) 39
MboII GAAGA 5 cut(s) 142, 245, 627, 775, 1051
MfeI CAATTG 3 cut(s) 222, 348, 1044
MflI RGATCY 3 cut(s) 259, 463, 1020
MhlI GDGCHC 1 cut(s) 1002
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 6 cut(s) 222, 348, 397, 742, 940, 1044
MluNI TGGCCA 1 cut(s) 341
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 4 cut(s) 194, 262, 308, 439
MmeI TCCRAC 1 cut(s) 977
Mox20I TGGCCA 1 cut(s) 341
MscI TGGCCA 1 cut(s) 341
MseI TTAA 1 cut(s) 857
Msp20I TGGCCA 1 cut(s) 341
MspI CCGG 3 cut(s) 57, 101, 156
MspR9I CCNGG 4 cut(s) 156, 265, 435, 1025
MunI CAATTG 3 cut(s) 222, 348, 1044
MvaI CCWGG 3 cut(s) 265, 435, 1025
MvnI CGCG 3 cut(s) 24, 65, 690
MwoI GCNNNNNNNGC 4 cut(s) 19, 435, 587, 793
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 610
NheI GCTAGC 1 cut(s) 566
NlaIII CATG 5 cut(s) 288, 614, 914, 985, 1007
NlaIV GGNNCC 4 cut(s) 61, 172, 921, 1022
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 469, 821
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 2 cut(s) 67, 766
PfoI TCCNGGA 1 cut(s) 1023
PkrI GCNGC 5 cut(s) 38, 64, 106, 689, 869
PleI GAGTC 4 cut(s) 193, 262, 307, 439
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 4 cut(s) 193, 262, 307, 439
Psp124BI GAGCTC 1 cut(s) 1002
Psp6I CCWGG 3 cut(s) 263, 433, 1023
PspFI CCCAGC 2 cut(s) 9, 833
PspGI CCWGG 3 cut(s) 263, 433, 1023
PspN4I GGNNCC 4 cut(s) 61, 172, 921, 1022
PspPI GGNCC 3 cut(s) 170, 332, 607
PsuI RGATCY 3 cut(s) 259, 463, 1020
RsaI GTAC 5 cut(s) 306, 707, 909, 1061, 1066
RsaNI GTAC 5 cut(s) 305, 706, 908, 1060, 1065
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 1 cut(s) 857
SatI GCNGC 5 cut(s) 37, 63, 105, 688, 868
Sau96I GGNCC 3 cut(s) 170, 332, 607
SchI GAGTC 4 cut(s) 194, 262, 308, 439
ScrFI CCNGG 4 cut(s) 156, 265, 435, 1025
SduI GDGCHC 1 cut(s) 1002
SfaNI GCATC 3 cut(s) 744, 783, 1018
SfcI CTRYAG 1 cut(s) 424
SfoI GGCGCC 1 cut(s) 61
SmlI CTYRAG 3 cut(s) 545, 571, 677
SmoI CTYRAG 3 cut(s) 545, 571, 677
Sse9I AATT 6 cut(s) 222, 348, 397, 742, 940, 1044
SsiI CCGC 3 cut(s) 37, 63, 688
SspDI GGCGCC 1 cut(s) 59
SspI AATATT 1 cut(s) 874
SspMI CTAG 3 cut(s) 108, 567, 788
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 4 cut(s) 154, 263, 433, 1023
StyI CCWWGG 3 cut(s) 603, 610, 1075
TaaI ACNGT 4 cut(s) 164, 503, 668, 1064
TaqI TCGA 1 cut(s) 448
TasI AATT 6 cut(s) 222, 348, 397, 742, 940, 1044
TatI WGTACW 1 cut(s) 907
TauI GCSGC 3 cut(s) 39, 65, 690
TfiI GAWTC 2 cut(s) 67, 766
Tru1I TTAA 1 cut(s) 857
Tru9I TTAA 1 cut(s) 857
TscAI CASTG 3 cut(s) 145, 387, 508
TseFI GTSAC 2 cut(s) 469, 821
TseI GCWGC 2 cut(s) 104, 867
Tsp45I GTSAC 2 cut(s) 469, 821
TspDTI ATGAA 2 cut(s) 357, 584
TspGWI ACGGA 3 cut(s) 178, 467, 503
TspRI CASTG 3 cut(s) 145, 387, 508
XapI RAATTY 2 cut(s) 397, 940
XspI CTAG 3 cut(s) 108, 567, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.