Rorug04G0187900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004342
Physical Location & Seq
Forward (+)
32651575 .. 32651841
267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0187900.1

Sequence Viewer

Length: 267 bp
ATGGGTATAGTTGACATGGGAGGCAGTGAGGCACCCCGATTCGAAGCCTCTTCCATTAAACCACCTGCAGAACAGAGACAACCGGTCGGCGTCCTCGACATCCGCTTCCCTGCTCATCCGGTCGTCGAGTTGCAGAGGCACCAGGCCAGCGTCAAAGCCGTTGCTTGGGCCAACCCATCTGCACTGCCGGGGACGACTCACAGGCCCTCATCTGGGACCTCTCCTCCATGGGCCAACCCGTCGAGGGTGGCCACGATCCCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

88

Amino Acids

9.35

Weight (kDa)

9.95

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 2 cut(s) 31, 138
AciI CCGC 1 cut(s) 103
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 4 cut(s) 165, 212, 213, 244
AgeI ACCGGT 1 cut(s) 82
AhdI GACNNNNNGTC 1 cut(s) 83
AjnI CCWGG 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 1 cut(s) 250
AoxI GGCC 5 cut(s) 144, 168, 203, 231, 249
AsiGI ACCGGT 1 cut(s) 82
AspS9I GGNCC 4 cut(s) 168, 204, 216, 231
AsuC2I CCSGG 1 cut(s) 189
AsuII TTCGAA 1 cut(s) 42
AvaII GGWCC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 2 cut(s) 31, 138
BccI CCATC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 143
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 70
BfmI CTRYAG 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 143, 189
Bme18I GGWCC 1 cut(s) 216
BmeRI GACNNNNNGTC 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 168, 204, 216, 231
BmiI GGNNCC 3 cut(s) 33, 140, 217
BmrFI CCNGG 2 cut(s) 143, 189
Bpu14I TTCGAA 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 188, 227
BsaWI WCCGGW 2 cut(s) 82, 118
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 4 cut(s) 165, 212, 213, 244
Bse118I RCCGGY 1 cut(s) 82
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 2 cut(s) 188, 227
BseGI GGATG 2 cut(s) 99, 115
BseLI CCNNNNNNNGG 4 cut(s) 165, 212, 213, 244
BseRI GAGGAG 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 165
Bsh1285I CGRYCG 2 cut(s) 87, 123
BshFI GGCC 5 cut(s) 146, 170, 205, 233, 251
BshNI GGYRCC 2 cut(s) 31, 138
BshTI ACCGGT 1 cut(s) 82
BsiEI CGRYCG 2 cut(s) 87, 123
BsiSI CCGG 3 cut(s) 83, 119, 188
BslFI GGGAC 2 cut(s) 205, 229
BslI CCNNNNNNNGG 4 cut(s) 165, 212, 213, 244
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 205, 229
BsnI GGCC 5 cut(s) 146, 170, 205, 233, 251
Bsp119I TTCGAA 1 cut(s) 42
Bsp143I GATC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 1 cut(s) 103
BspANI GGCC 5 cut(s) 146, 170, 205, 233, 251
BspLI GGNNCC 3 cut(s) 33, 140, 217
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 1 cut(s) 250
BspT104I TTCGAA 1 cut(s) 42
BspT107I GGYRCC 2 cut(s) 31, 138
BsrFI RCCGGY 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 188, 227
BssMI GATC 1 cut(s) 255
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 55
BstACI GRCGYC 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 148
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 99, 115
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 1 cut(s) 255
BstMCI CGRYCG 2 cut(s) 87, 123
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 141, 187
BstSFI CTRYAG 1 cut(s) 66
BsuRI GGCC 5 cut(s) 146, 170, 205, 233, 251
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 2 cut(s) 99, 115
BtsI GCAGTG 2 cut(s) 31, 182
BtsIMutI CAGTG 2 cut(s) 31, 182
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 148
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 4 cut(s) 168, 204, 216, 231
CseI GACGC 2 cut(s) 79, 139
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 2 cut(s) 16, 228
CviJI RGCY 7 cut(s) 47, 146, 158, 170, 205, 233, 251
CviKI_1 RGCY 7 cut(s) 47, 146, 158, 170, 205, 233, 251
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
DriI GACNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 55
Eam1105I GACNNNNNGTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 204, 216
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 2 cut(s) 19, 231
FaiI YATR 3 cut(s) 8, 17, 229
FaqI GGGAC 2 cut(s) 205, 229
FatI CATG 2 cut(s) 15, 227
FokI GGATG 2 cut(s) 86, 102
HaeIII GGCC 5 cut(s) 146, 170, 205, 233, 251
HapII CCGG 3 cut(s) 83, 119, 188
HgaI GACGC 2 cut(s) 79, 139
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 2 cut(s) 19, 231
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 39, 196
HpaII CCGG 3 cut(s) 83, 119, 188
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 266
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 2 cut(s) 128, 244
HpyCH4V TGCA 3 cut(s) 68, 133, 182
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 2 cut(s) 19, 231
Kzo9I GATC 1 cut(s) 255
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 251
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 9 cut(s) 14, 22, 58, 104, 129, 217, 229, 234, 237
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 57
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 3 cut(s) 83, 119, 188
MspR9I CCNGG 2 cut(s) 143, 189
MvaI CCWGG 1 cut(s) 143
NciI CCSGG 1 cut(s) 189
NcoI CCATGG 1 cut(s) 227
NdeII GATC 1 cut(s) 255
NlaIII CATG 2 cut(s) 19, 231
NlaIV GGNNCC 3 cut(s) 33, 140, 217
NspV TTCGAA 1 cut(s) 42
PaqCI CACCTGC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 39
PinAI ACCGGT 1 cut(s) 82
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 3 cut(s) 33, 140, 217
PspPI GGNCC 4 cut(s) 168, 204, 216, 231
PspPPI RGGWCCY 1 cut(s) 216
PstI CTGCAG 1 cut(s) 70
SaqAI TTAA 1 cut(s) 57
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 4 cut(s) 168, 204, 216, 231
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 143, 189
SetI ASST 2 cut(s) 67, 221
SfcI CTRYAG 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 42
SinI GGWCC 1 cut(s) 216
SsiI CCGC 1 cut(s) 103
StyD4I CCNGG 2 cut(s) 141, 187
StyI CCWWGG 1 cut(s) 227
TaqI TCGA 4 cut(s) 42, 96, 126, 242
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 2 cut(s) 31, 189
TspRI CASTG 2 cut(s) 31, 189
VpaK11BI GGWCC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.