Rh4BG252000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004342
Physical Location & Seq
Forward (+)
42209955 .. 42211096
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG252000.1

Sequence Viewer

Length: 345 bp
ATGGGGAATGTTCAAGTTTCTGTTGCTCCTCCTACACGGCCTCCTCCAAGAACTGTTCCTAATTACACTCGTGGGGGACATGATACCGATCGGCATGCATATGCCAACACGAACATGGAGAATCGTGACCAAATTCCAGGAGTAGGAGTGCAGAAGCAAAGACCCGTCATCAACTCTGATGAGGCTGCGCGACGCTACGGTGGGTTTTTCTACCTTGTTGCTTTCGAAGATACAATTCTTCACCTATTATTGGATCTTAACCGATGTGCAAAAATGCACTTGAAAAAGGTTGAAAACATCCATTCGAGAAAAAAGCGAACAATGGCACCAACGATGAATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.94

Weight (kDa)

10.0

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AccII CGCG 1 cut(s) 190
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 1 cut(s) 132
AfiI CCNNNNNNNGG 2 cut(s) 143, 250
AgsI TTSAA 3 cut(s) 14, 283, 293
AjnI CCWGG 1 cut(s) 136
AlwI GGATC 1 cut(s) 261
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 132
AspLEI GCGC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 233
AsuII TTCGAA 1 cut(s) 225
BanI GGYRCC 1 cut(s) 325
BauI CACGAG 1 cut(s) 69
BbvI GCAGC 1 cut(s) 172
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 1 cut(s) 138
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 138
Bpu14I TTCGAA 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 87
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 250
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 297
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 143, 250
BseRI GAGGAG 2 cut(s) 18, 33
BseXI GCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 190
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 143, 250
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 1 cut(s) 40
Bsp119I TTCGAA 1 cut(s) 225
Bsp143I GATC 2 cut(s) 88, 253
BspANI GGCC 1 cut(s) 40
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 327
BspPI GGATC 1 cut(s) 261
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 325
BssMI GATC 2 cut(s) 88, 253
BssSI CACGAG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 55, 200
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 2 cut(s) 91, 256
BstMBI GATC 2 cut(s) 88, 253
BstMCI CGRYCG 1 cut(s) 91
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 136
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 40
BtsCI GGATG 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 190
CseI GACGC 1 cut(s) 201
CviAII CATG 3 cut(s) 80, 95, 115
CviJI RGCY 2 cut(s) 40, 185
CviKI_1 RGCY 2 cut(s) 40, 185
DdeI CTNAG 1 cut(s) 342
DpnI GATC 2 cut(s) 90, 255
DpnII GATC 2 cut(s) 88, 253
EcoRII CCWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 100
FaeI CATG 3 cut(s) 83, 98, 118
FaiI YATR 5 cut(s) 81, 96, 100, 102, 116
FaqI GGGAC 1 cut(s) 90
FatI CATG 3 cut(s) 79, 94, 114
FauNDI CATATG 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 186
GlaI GCGC 1 cut(s) 189
GluI GCNGC 1 cut(s) 186
HaeIII GGCC 1 cut(s) 40
HgaI GACGC 1 cut(s) 201
HhaI GCGC 1 cut(s) 190
Hin1II CATG 3 cut(s) 83, 98, 118
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 2 cut(s) 121, 337
HphI GGTGA 1 cut(s) 233
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 2 cut(s) 125, 306
Hpy99I CGWCG 1 cut(s) 195
HpyCH4III ACNGT 2 cut(s) 55, 200
HpyCH4V TGCA 4 cut(s) 98, 151, 269, 277
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 3 cut(s) 83, 98, 118
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 2 cut(s) 88, 253
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 2 cut(s) 123, 150
Lsp1109I GCAGC 1 cut(s) 172
MaeIII GTNAC 1 cut(s) 125
MalI GATC 2 cut(s) 90, 255
MboI GATC 2 cut(s) 88, 253
MboII GAAGA 2 cut(s) 230, 239
MflI RGATCY 1 cut(s) 253
MluCI AATT 3 cut(s) 61, 132, 234
MnlI CCTC 4 cut(s) 39, 51, 54, 175
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 2 cut(s) 99, 113
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 190
NdeI CATATG 1 cut(s) 100
NdeII GATC 2 cut(s) 88, 253
NlaIII CATG 3 cut(s) 83, 98, 118
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 100
NspI RCATGY 1 cut(s) 98
NspV TTCGAA 1 cut(s) 225
PaeI GCATGC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 121, 337
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 1 cut(s) 187
Ple19I CGATCG 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 327
PsuI RGATCY 1 cut(s) 253
PvuI CGATCG 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 99, 113
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 88, 253
ScrFI CCNGG 1 cut(s) 138
SetI ASST 3 cut(s) 216, 246, 291
SfuI TTCGAA 1 cut(s) 225
SmiMI CAYNNNNRTG 2 cut(s) 99, 113
SphI GCATGC 1 cut(s) 98
Sse9I AATT 3 cut(s) 61, 132, 234
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 55, 200
TaqI TCGA 2 cut(s) 225, 305
TasI AATT 3 cut(s) 61, 132, 234
TfiI GAWTC 2 cut(s) 121, 337
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 125
XapI RAATTY 1 cut(s) 132
XceI RCATGY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.