Rh4AG247000

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004342
Physical Location & Seq
Forward (+)
55916139 .. 55919853
3715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG247000.1

Sequence Viewer

Length: 441 bp
ATGGCAACGCAAGAAGACCTAGCAAAGCTTGGGATGGAGGCTTTTGCTATGCTAGACAAGTACTATGGCCGGCCCACTGCCGGAGGGGGAATCAGTACTCCATTTCCTTATTCTCATGAAGATCATCACCAACCTCGAGCTCATCCTCATCACATACTAGCCGGAAGACCACGTCCTCAGCAGGCCGGTGTTATTAACAGCAAGGATGCAGCTCTGGCCTTTGGAGGGTTTGAGATCAAAGAATATGACCAAAAACGGTTTCGACCACCTCCGACCCGGCCACAACCAAGGACTACTGTTACTCCTCCTAACTACCCTAGTCCAGGAGCACCGCAGAAGCAGCCCATCAACTCTGACGAGGCAGCACGACGCTACGGTGGGATTGTCTTTACTGAATATGGTACTACTACGAATAATCCAACTGTCAATAGGCGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.17

Weight (kDa)

9.64

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 332
AcoI YGGCCR 2 cut(s) 67, 278
AfaI GTAC 3 cut(s) 62, 97, 403
AfiI CCNNNNNNNGG 3 cut(s) 80, 225, 323
AjiI CACGTC 1 cut(s) 173
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 3 cut(s) 28, 140, 212
AluI AGCT 3 cut(s) 28, 140, 212
Alw21I GWGCWC 2 cut(s) 142, 331
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 5 cut(s) 67, 71, 183, 216, 278
ApeKI GCWGC 3 cut(s) 209, 340, 362
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 135
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 21, 172
Bbv12I GWGCWC 2 cut(s) 142, 331
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 3 cut(s) 221, 352, 374
BccI CCATC 2 cut(s) 28, 353
BciT130I CCWGG 1 cut(s) 324
BcnI CCSGG 1 cut(s) 277
BfaI CTAG 4 cut(s) 20, 53, 158, 318
BisI GCNGC 3 cut(s) 210, 341, 363
BlsI GCNGC 3 cut(s) 211, 342, 364
BmcAI AGTACT 2 cut(s) 62, 97
Bme1390I CCNGG 2 cut(s) 277, 324
BmeT110I CYCGRG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 277, 324
BmsI GCATC 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 21, 172
Bpu10I CCTNAGC 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 225, 323
Bse118I RCCGGY 2 cut(s) 69, 185
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 3 cut(s) 39, 142, 211
BseLI CCNNNNNNNGG 3 cut(s) 80, 225, 323
BseMII CTCAG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 294
BseXI GCAGC 3 cut(s) 221, 352, 374
BshFI GGCC 5 cut(s) 69, 73, 185, 218, 280
BsiHKAI GWGCWC 2 cut(s) 142, 331
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 5 cut(s) 70, 81, 162, 186, 277
BslI CCNNNNNNNGG 3 cut(s) 80, 225, 323
BsnI GGCC 5 cut(s) 69, 73, 185, 218, 280
BsoBI CYCGRG 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 142, 331
Bsp143I GATC 2 cut(s) 121, 234
BspACI CCGC 1 cut(s) 332
BspANI GGCC 5 cut(s) 69, 73, 185, 218, 280
BspCNI CTCAG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 69, 185
BssAI RCCGGY 2 cut(s) 69, 185
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 2 cut(s) 121, 234
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 4 cut(s) 258, 298, 377, 424
BstC8I GCNNGC 2 cut(s) 71, 183
BstDEI CTNAG 2 cut(s) 177, 438
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 39, 142, 211
BstKTI GATC 2 cut(s) 124, 237
BstMBI GATC 2 cut(s) 121, 234
BstMWI GCNNNNNNNGC 2 cut(s) 215, 340
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 275, 322
BstV1I GCAGC 3 cut(s) 221, 352, 374
BstV2I GAAGAC 2 cut(s) 21, 172
BsuRI GGCC 5 cut(s) 69, 73, 185, 218, 280
BtrI CACGTC 1 cut(s) 173
BtsCI GGATG 3 cut(s) 39, 142, 211
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 2 cut(s) 71, 183
CciI TCATGA 1 cut(s) 115
Cfr10I RCCGGY 2 cut(s) 69, 185
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 378
Csp6I GTAC 3 cut(s) 61, 96, 402
CviAII CATG 1 cut(s) 116
CviQI GTAC 3 cut(s) 61, 96, 402
DdeI CTNAG 2 cut(s) 177, 438
DpnI GATC 2 cut(s) 123, 236
DpnII GATC 2 cut(s) 121, 234
EaeI YGGCCR 2 cut(s) 67, 278
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 140
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 1 cut(s) 119
FaiI YATR 6 cut(s) 50, 66, 117, 155, 246, 399
FatI CATG 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 210, 341, 363
FokI GGATG 3 cut(s) 46, 129, 218
FriOI GRGCYC 1 cut(s) 142
FseI GGCCGGCC 1 cut(s) 73
Fsp4HI GCNGC 3 cut(s) 210, 341, 363
FspBI CTAG 4 cut(s) 20, 53, 158, 318
GluI GCNGC 3 cut(s) 210, 341, 363
HaeIII GGCC 5 cut(s) 69, 73, 185, 218, 280
HapII CCGG 5 cut(s) 70, 81, 162, 186, 277
HgaI GACGC 1 cut(s) 378
Hin1II CATG 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 90
HpaII CCGG 5 cut(s) 70, 81, 162, 186, 277
HphI GGTGA 1 cut(s) 119
Hpy188I TCNGA 2 cut(s) 273, 355
Hpy188III TCNNGA 1 cut(s) 116
Hpy99I CGWCG 1 cut(s) 372
HpyCH4III ACNGT 4 cut(s) 258, 298, 377, 424
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 340
HpyF3I CTNAG 2 cut(s) 177, 438
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 1 cut(s) 119
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Kzo9I GATC 2 cut(s) 121, 234
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 9 cut(s) 83, 94, 167, 175, 199, 200, 290, 309, 336
Lsp1109I GCAGC 3 cut(s) 221, 352, 374
LweI GCATC 1 cut(s) 196
MaeI CTAG 4 cut(s) 20, 53, 158, 318
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 123, 236
MboI GATC 2 cut(s) 121, 234
MboII GAAGA 3 cut(s) 26, 131, 177
MhlI GDGCHC 2 cut(s) 142, 331
MmeI TCCRAC 1 cut(s) 296
MnlI CCTC 9 cut(s) 31, 77, 144, 156, 186, 218, 279, 315, 352
MroNI GCCGGC 1 cut(s) 69
MseI TTAA 1 cut(s) 195
MspI CCGG 5 cut(s) 70, 81, 162, 186, 277
MspR9I CCNGG 2 cut(s) 277, 324
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 2 cut(s) 215, 340
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 277
NdeII GATC 2 cut(s) 121, 234
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 135
PagI TCATGA 1 cut(s) 115
PdiI GCCGGC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 90
PflFI GACNNNGTC 1 cut(s) 171
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 3 cut(s) 211, 342, 364
Psp124BI GAGCTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspPI GGNCC 1 cut(s) 72
PspXI VCTCGAGB 1 cut(s) 135
PsyI GACNNNGTC 1 cut(s) 171
RigI GGCCGGCC 1 cut(s) 73
RsaI GTAC 3 cut(s) 62, 97, 403
RsaNI GTAC 3 cut(s) 61, 96, 402
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 210, 341, 363
Sau3AI GATC 2 cut(s) 121, 234
Sau96I GGNCC 1 cut(s) 72
ScaI AGTACT 2 cut(s) 62, 97
ScrFI CCNGG 2 cut(s) 277, 324
SduI GDGCHC 2 cut(s) 142, 331
SetI ASST 7 cut(s) 21, 30, 136, 142, 175, 214, 271
SfaNI GCATC 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 135
SlaI CTCGAG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
SsiI CCGC 1 cut(s) 332
SspMI CTAG 4 cut(s) 20, 53, 158, 318
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 2 cut(s) 275, 322
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 4 cut(s) 258, 298, 377, 424
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 136, 262
TatI WGTACW 2 cut(s) 60, 95
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 82
TseI GCWGC 3 cut(s) 209, 340, 362
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 1 cut(s) 82
Tth111I GACNNNGTC 1 cut(s) 171
XagI CCTNNNNNAGG 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 135
XspI CTAG 4 cut(s) 20, 53, 158, 318
ZrmI AGTACT 2 cut(s) 62, 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.