Rorug06G0072200

protein At4g18490 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001455
Physical Location & Seq
Reverse (-)
9595565 .. 9596164
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0072200.1

Sequence Viewer

Length: 333 bp
ATGCCGAGTTTCGCCTCCGAAAACGGGCGGCTGGGCAGCGACTTCTTGTGGCTGGGATTCAGCATCAAGAACGGATCGCTGGCAAATGCCTCCATGATGAAGTTCTGTGTCCACTCGGGCACTCACGTCGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGATTTGATGTCGATGCTTTAGACCTCGAAGTGTTAAACGGCCCCGCGCTGGTGGTTGATGTTCCCAGGGATAAGAACATAACTGCTGAAGTAATGAAGGCTTTGAACATTCCTAAAGGAGTGCGTCGTGTGCTTTTCAGAACCTTAAATACTGACAGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

12.12

Weight (kDa)

6.16

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 31 - 104 1.6e-10 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 28, 213
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 276
AfiI CCNNNNNNNGG 2 cut(s) 24, 217
AgsI TTSAA 1 cut(s) 274
AjiI CACGTC 2 cut(s) 127, 145
AjnI CCWGG 2 cut(s) 136, 233
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 48
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 2 cut(s) 138, 235
BisI GCNGC 2 cut(s) 29, 37
BlsI GCNGC 2 cut(s) 30, 38
Bme1390I CCNGG 2 cut(s) 138, 235
BmeT110I CYCGRG 1 cut(s) 115
BmgBI CACGTC 2 cut(s) 127, 145
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 138, 235
BmsI GCATC 2 cut(s) 72, 172
BpiI GAAGAC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 233, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 217
BseBI CCWGG 2 cut(s) 138, 235
BseDI CCNNGG 2 cut(s) 233, 234
BseLI CCNNNNNNNGG 2 cut(s) 24, 217
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 2 cut(s) 31, 52
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 24, 217
BsnI GGCC 1 cut(s) 210
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 1 cut(s) 74
BspACI CCGC 2 cut(s) 28, 213
BspANI GGCC 1 cut(s) 210
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 211
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 233, 234
BssMI GATC 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 138, 235
BstC8I GCNNGC 1 cut(s) 81
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 77
BstMBI GATC 1 cut(s) 74
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 2 cut(s) 138, 235
BstSCI CCNGG 2 cut(s) 136, 233
BstSLI GKGCMC 1 cut(s) 122
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 210
BtrI CACGTC 2 cut(s) 127, 145
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 2 cut(s) 140, 281
CsiI ACCWGGT 1 cut(s) 136
CviAII CATG 2 cut(s) 94, 164
CviJI RGCY 5 cut(s) 31, 52, 210, 269, 327
CviKI_1 RGCY 5 cut(s) 31, 52, 210, 269, 327
DpnI GATC 1 cut(s) 76
DpnII GATC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 136, 233
FaeI CATG 2 cut(s) 97, 167
FaiI YATR 3 cut(s) 95, 165, 248
FatI CATG 2 cut(s) 93, 163
FauI CCCGC 1 cut(s) 220
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 29, 37
Fsp4HI GCNGC 2 cut(s) 29, 37
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 29, 37
GsaI CCCAGC 2 cut(s) 35, 56
HaeIII GGCC 1 cut(s) 210
HgaI GACGC 2 cut(s) 140, 281
HhaI GCGC 1 cut(s) 217
Hin1II CATG 2 cut(s) 97, 167
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 112, 130
Hpy188I TCNGA 2 cut(s) 19, 308
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 2 cut(s) 112, 130
Hpy99I CGWCG 3 cut(s) 131, 134, 297
HpyAV CCTTC 1 cut(s) 259
HpyCH4IV ACGT 2 cut(s) 126, 144
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpySE526I ACGT 2 cut(s) 126, 144
Hsp92II CATG 2 cut(s) 97, 167
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 2 cut(s) 72, 172
MabI ACCWGGT 1 cut(s) 136
MaeII ACGT 2 cut(s) 126, 144
MaeIII GTNAC 1 cut(s) 140
MalI GATC 1 cut(s) 76
MboI GATC 1 cut(s) 74
MboII GAAGA 1 cut(s) 139
MhlI GDGCHC 1 cut(s) 122
MnlI CCTC 3 cut(s) 25, 100, 203
MseI TTAA 2 cut(s) 203, 314
MspA1I CMGCKG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 138, 235
MvaI CCWGG 2 cut(s) 138, 235
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 298
NdeII GATC 1 cut(s) 74
NlaIII CATG 2 cut(s) 97, 167
NlaIV GGNNCC 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 234
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 2 cut(s) 30, 38
Psp6I CCWGG 2 cut(s) 136, 233
PspFI CCCAGC 2 cut(s) 31, 52
PspGI CCWGG 2 cut(s) 136, 233
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 209
PsrI GAACNNNNNNTAC 2 cut(s) 301, 333
PvuII CAGCTG 1 cut(s) 327
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 2 cut(s) 203, 314
SatI GCNGC 2 cut(s) 29, 37
Sau3AI GATC 1 cut(s) 74
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 138, 235
SduI GDGCHC 1 cut(s) 122
SetI ASST 6 cut(s) 129, 139, 147, 195, 314, 329
SexAI ACCWGGT 1 cut(s) 136
SfaNI GCATC 2 cut(s) 72, 172
SgrDI CGTCGACG 1 cut(s) 128
SsiI CCGC 2 cut(s) 28, 213
StyD4I CCNGG 2 cut(s) 136, 233
TaiI ACGT 2 cut(s) 129, 147
TaqI TCGA 4 cut(s) 129, 150, 180, 195
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 2 cut(s) 203, 314
Tru9I TTAA 2 cut(s) 203, 314
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 1 cut(s) 36
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 113, 278
TspGWI ACGGA 1 cut(s) 87
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.