Rorug07G0154900

protein At4g18490 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001455
Physical Location & Seq
Forward (+)
12256590 .. 12257228
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0154900.1

Sequence Viewer

Length: 639 bp
ATGACTGAAAAGATCAAAAACGCCTTGGCATCAGTCAAAGGTTACACTACCAGAAAAGATGAACAAATGCCCACCTTCCAATCCGAGGAGGAACTCAAACGCCAGAAGAGGATCAAGTTGTTTACATATATTGGCATTTTCATAGTGTTTCAAATCATAGTCATGACTGTATTTGGTCTCACTATCATGAAAGTGAAGACTCCAAAGGTGAGATTGGGCACCACCAATGTCCAAAATCTCAACTTTGTTCCCACGACTCCTTCATTTGACACAACCTTTGCAACCCAAATCAGGGTCAAGAACACAAATTGGGGTGCCTATAAGTTCGATGCAGGCATTGCAACGTTCATGTACCAAGGTGTAGCAGTGGGGCAAGTTTCTATTCCAAAGAGCAAGGCCGGAATGCGTTCCACCAAAAAGATCAATGTCGAGGTGAGTTTGAATTCCAATGGATTGCCAAGCACTTCCAATCTTGGAAGTGAATTGAGCAGTGGCGTATTAACTTTGACCAGCGAAGCTAAATTGACTGGAAAAGTTGAATTGATGCTTATAATGAAAAAGAAGAAAGCCGCCACAATGAACTGTGCCATGAAACTTGATTTGTCCACAAAGACAATTCAAGCTTTGGAATGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.44

Weight (kDa)

9.98

Isoelectric Point (pI)

22.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 97 - 195 4.2e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
AccB1I GGYRCC 2 cut(s) 218, 314
AciI CCGC 1 cut(s) 570
AclI AACGTT 1 cut(s) 344
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 1 cut(s) 442
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 85, 291, 292
AgsI TTSAA 4 cut(s) 152, 442, 539, 620
AluBI AGCT 2 cut(s) 518, 623
AluI AGCT 2 cut(s) 518, 623
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 396
ApoI RAATTY 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 406
AsuHPI GGTGA 2 cut(s) 220, 445
BaeGI GKGCMC 1 cut(s) 221
BanI GGYRCC 2 cut(s) 218, 314
BbsI GAAGAC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 571
BmiI GGNNCC 2 cut(s) 220, 316
BmsI GCATC 3 cut(s) 38, 319, 534
BpiI GAAGAC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 24, 84, 355
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 291, 292
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 24, 84, 355
BseLI CCNNNNNNNGG 3 cut(s) 85, 291, 292
BseMI GCAATG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 221
BshFI GGCC 1 cut(s) 398
BshNI GGYRCC 2 cut(s) 218, 314
BsiSI CCGG 1 cut(s) 399
BslI CCNNNNNNNGG 3 cut(s) 85, 291, 292
BsmAI GTCTC 1 cut(s) 182
BsmI GAATGC 2 cut(s) 408, 635
BsnI GGCC 1 cut(s) 398
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 3 cut(s) 12, 111, 420
BspACI CCGC 1 cut(s) 570
BspANI GGCC 1 cut(s) 398
BspHI TCATGA 2 cut(s) 162, 186
BspLI GGNNCC 2 cut(s) 220, 316
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 218, 314
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 24, 84, 355
BssMI GATC 3 cut(s) 12, 111, 420
BssT1I CCWWGG 2 cut(s) 24, 355
Bst4CI ACNGT 2 cut(s) 169, 584
Bst6I CTCTTC 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 334
BstKTI GATC 3 cut(s) 15, 114, 423
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 3 cut(s) 12, 111, 420
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstSLI GKGCMC 1 cut(s) 221
BstV2I GAAGAC 1 cut(s) 203
BsuRI GGCC 1 cut(s) 398
BtsI GCAGTG 2 cut(s) 372, 496
BtsIMutI CAGTG 2 cut(s) 372, 496
Cac8I GCNNGC 1 cut(s) 334
CciI TCATGA 2 cut(s) 162, 186
Csp6I GTAC 1 cut(s) 352
CviAII CATG 4 cut(s) 163, 187, 349, 589
CviJI RGCY 4 cut(s) 398, 518, 569, 623
CviKI_1 RGCY 4 cut(s) 398, 518, 569, 623
CviQI GTAC 1 cut(s) 352
DpnI GATC 3 cut(s) 14, 113, 422
DpnII GATC 3 cut(s) 12, 111, 420
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 24, 355
Eco31I GGTCTC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 24, 355
ErhI CCWWGG 2 cut(s) 24, 355
FaeI CATG 4 cut(s) 166, 190, 352, 592
FatI CATG 4 cut(s) 162, 186, 348, 588
Fnu4HI GCNGC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 570
GluI GCNGC 1 cut(s) 570
HaeIII GGCC 1 cut(s) 398
HapII CCGG 1 cut(s) 399
Hin1II CATG 4 cut(s) 166, 190, 352, 592
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 2 cut(s) 199, 256
HpaII CCGG 1 cut(s) 399
HphI GGTGA 2 cut(s) 220, 445
Hpy166II GTNNAC 2 cut(s) 123, 606
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 3 cut(s) 163, 187, 298
Hpy8I GTNNAC 2 cut(s) 123, 606
HpyAV CCTTC 2 cut(s) 85, 270
HpyCH4III ACNGT 2 cut(s) 169, 584
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 281, 332, 341, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 4 cut(s) 166, 190, 352, 592
Kzo9I GATC 3 cut(s) 12, 111, 420
LpnPI CCDG 7 cut(s) 64, 116, 277, 318, 412, 513, 523
LweI GCATC 3 cut(s) 38, 319, 534
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 41
MalI GATC 3 cut(s) 14, 113, 422
MboI GATC 3 cut(s) 12, 111, 420
MboII GAAGA 3 cut(s) 118, 208, 574
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 6 cut(s) 307, 442, 482, 521, 539, 615
MlyI GAGTC 2 cut(s) 193, 250
MnlI CCTC 4 cut(s) 79, 82, 102, 424
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 1 cut(s) 500
MslI CAYNNNNRTG 3 cut(s) 161, 185, 191
MspI CCGG 1 cut(s) 399
Mva1269I GAATGC 2 cut(s) 408, 635
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 3 cut(s) 12, 111, 420
NlaIII CATG 4 cut(s) 166, 190, 352, 592
NlaIV GGNNCC 2 cut(s) 220, 316
PagI TCATGA 2 cut(s) 162, 186
PctI GAATGC 2 cut(s) 408, 635
PdmI GAANNNNTTC 1 cut(s) 406
PkrI GCNGC 1 cut(s) 571
PleI GAGTC 2 cut(s) 193, 250
PpsI GAGTC 2 cut(s) 193, 250
PsiI TTATAA 1 cut(s) 551
Psp1406I AACGTT 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 220, 316
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 3 cut(s) 161, 185, 191
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 3 cut(s) 12, 111, 420
SchI GAGTC 2 cut(s) 193, 250
SduI GDGCHC 1 cut(s) 221
SetI ASST 9 cut(s) 43, 77, 210, 278, 347, 361, 435, 520, 625
SfaNI GCATC 3 cut(s) 38, 319, 534
SmiMI CAYNNNNRTG 3 cut(s) 161, 185, 191
Sse9I AATT 6 cut(s) 307, 442, 482, 521, 539, 615
SsiI CCGC 1 cut(s) 570
StyI CCWWGG 2 cut(s) 24, 355
TaaI ACNGT 2 cut(s) 169, 584
TaiI ACGT 1 cut(s) 347
TaqI TCGA 2 cut(s) 327, 429
TasI AATT 6 cut(s) 307, 442, 482, 521, 539, 615
TauI GCSGC 1 cut(s) 572
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TscAI CASTG 2 cut(s) 372, 496
TspDTI ATGAA 8 cut(s) 75, 130, 203, 252, 337, 569, 593, 605
TspRI CASTG 2 cut(s) 372, 496
XapI RAATTY 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.