Rh2DG021400

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007544
Physical Location & Seq
Reverse (-)
1245154 .. 1246086
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG021400.1

Sequence Viewer

Length: 906 bp
ATGGCTCTTGATTTTTTGGGAGGAGCACCCTTTCAGCTGCTTTATGATGGGGTGAAGCTCGCACTTCAGAACTCGAAGAGCTTCAAGACTCAGTTGAAGAACCTGGAATTCACTTTAAGAGCACTTCAGCCAGCTGCAGTGGAGCAGCTCCGATTAAACAACATACATCTCAGCCTTTCTACTAGATACCTAGAAGAGTTAGAGAAGAAAATGAACGACGGTGTTCAGCTTCTTAGCCAAATGTCAAGTGTTAGCGGTTTAAATGTGCACGTATGGGGCTGTTGCAAGAGGAGTTATGCACAAGACCTTGAAAATTTAAACCAGGAGCTTCAAATTTTTCTAGAAAGGCCTAGGGTGGAGTCAATGATACTTACGAGACAGATTGCCATTTGGACCAAGGCACTATACGACGACCGGGATGATGGAACGGGGAAAAATCAGGTTAGCAATCAGCCCAATGATATAATTGGCCAAGATGTTTCGGCAAGAGAAGGGCCTGCTGTTCCTTCTCTGATATCACAACTTCTGAGTGCATTTCGTGCCTTCTCCGATGCCCTACAAGGAAATTCAAAAAGTTGCAAGATTTATACACTGCTGGAACTGAGTCTAAAGTGTCTGGAACCATTGCTGTTACTGCTATCTACATATGGAAAGGCCTTCCATTCAAAGGAAGAACTGTTGAAGCTTGTTGGCAAATTTTGGAATAGGTACAATAGTGATTACAAGGACAAGCTCTCTCAGTTAGACTTTGATCTCCAAGACCTCTTCCTTATACTGTTCTTGGAAATTGAAAATGATTTAAAGAGCTTGCAATGGGAGATCGATCAGCAGCGTGCTTTGGTTCCAGCATGTGAAGGGCAATCAGTATCTGAAGTTGACACTGTAGCAGCGGAATCAGTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

301

Amino Acids

34.0

Weight (kDa)

5.09

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 115 1e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 255, 890
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 5 cut(s) 107, 313, 333, 565, 695
AcuI CTGAAG 3 cut(s) 50, 110, 891
AfaI GTAC 1 cut(s) 710
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 8 cut(s) 85, 97, 311, 332, 570, 666, 682, 791
AjnI CCWGG 2 cut(s) 102, 321
Alw21I GWGCWC 3 cut(s) 28, 124, 270
Alw26I GTCTC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 869
AoxI GGCC 4 cut(s) 347, 469, 494, 654
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 5 cut(s) 37, 134, 145, 829, 887
ApoI RAATTY 5 cut(s) 107, 313, 333, 565, 695
Asp700I GAANNNNTTC 1 cut(s) 80
AspA2I CCTAGG 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 393, 494
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 1 cut(s) 393
AvrII CCTAGG 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 270
BaeI ACNNNNGTAYC 2 cut(s) 700, 733
BalI TGGCCA 1 cut(s) 471
Bbv12I GWGCWC 3 cut(s) 28, 124, 270
BbvI GCAGC 5 cut(s) 24, 121, 157, 841, 899
BccI CCATC 2 cut(s) 41, 416
BciT130I CCWGG 2 cut(s) 104, 323
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 4 cut(s) 183, 191, 341, 351
BfmI CTRYAG 2 cut(s) 135, 882
BisI GCNGC 5 cut(s) 38, 135, 146, 830, 888
BlnI CCTAGG 1 cut(s) 350
BlsI GCNGC 5 cut(s) 39, 136, 147, 831, 889
Bme1390I CCNGG 3 cut(s) 104, 323, 416
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 393, 494
BmiI GGNNCC 2 cut(s) 621, 843
BmrFI CCNGG 3 cut(s) 104, 323, 416
BmsI GCATC 1 cut(s) 541
BpuMI CCSGG 1 cut(s) 416
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 350, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse3DI GCAATG 2 cut(s) 623, 818
BseBI CCWGG 2 cut(s) 104, 323
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 2 cut(s) 350, 396
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMI GCAATG 2 cut(s) 623, 818
BseMII CTCAG 5 cut(s) 104, 184, 518, 593, 752
BseRI GAGGAG 2 cut(s) 36, 304
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 5 cut(s) 24, 121, 157, 841, 899
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 4 cut(s) 349, 471, 496, 656
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 3 cut(s) 28, 124, 270
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 1 cut(s) 370
BsnI GGCC 4 cut(s) 349, 471, 496, 656
Bsp1286I GDGCHC 3 cut(s) 28, 124, 270
Bsp143I GATC 3 cut(s) 751, 819, 823
BspACI CCGC 2 cut(s) 255, 890
BspANI GGCC 4 cut(s) 349, 471, 496, 656
BspCNI CTCAG 5 cut(s) 103, 183, 519, 594, 751
BspDI ATCGAT 1 cut(s) 822
BspLI GGNNCC 2 cut(s) 621, 843
BspMAI CTGCAG 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 623, 818
BssECI CCNNGG 2 cut(s) 350, 396
BssMI GATC 3 cut(s) 751, 819, 823
BssT1I CCWWGG 2 cut(s) 350, 396
Bst2UI CCWGG 2 cut(s) 104, 323
Bst4CI ACNGT 4 cut(s) 221, 678, 777, 883
Bst6I CTCTTC 3 cut(s) 71, 189, 770
BstAPI GCANNNNNTGC 1 cut(s) 539
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 5 cut(s) 60, 132, 498, 809, 834
BstDEI CTNAG 6 cut(s) 90, 170, 233, 527, 602, 738
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 3 cut(s) 754, 822, 826
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 3 cut(s) 751, 819, 823
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 2 cut(s) 539, 634
BstNI CCWGG 2 cut(s) 104, 323
BstNSI RCATGY 1 cut(s) 852
BstSCI CCNGG 3 cut(s) 102, 321, 414
BstSFI CTRYAG 2 cut(s) 135, 882
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 5 cut(s) 24, 121, 157, 841, 899
Bsu15I ATCGAT 1 cut(s) 822
BsuRI GGCC 4 cut(s) 349, 471, 496, 656
BsuTUI ATCGAT 1 cut(s) 822
BtsCI GGATG 1 cut(s) 424
BtsI GCAGTG 2 cut(s) 144, 590
BtsIMutI CAGTG 3 cut(s) 144, 590, 879
Cac8I GCNNGC 5 cut(s) 60, 132, 498, 809, 834
CaiI CAGNNNCTG 1 cut(s) 869
Cfr13I GGNCC 2 cut(s) 393, 494
ClaI ATCGAT 1 cut(s) 822
Csp6I GTAC 1 cut(s) 709
CviAII CATG 1 cut(s) 849
CviQI GTAC 1 cut(s) 709
DdeI CTNAG 6 cut(s) 90, 170, 233, 527, 602, 738
DpnI GATC 3 cut(s) 753, 821, 825
DpnII GATC 3 cut(s) 751, 819, 823
DraI TTTAAA 3 cut(s) 261, 318, 801
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 3 cut(s) 71, 189, 770
EarI CTCTTC 3 cut(s) 71, 189, 770
Eco130I CCWWGG 2 cut(s) 350, 396
Eco147I AGGCCT 2 cut(s) 349, 656
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 393
Eco57I CTGAAG 3 cut(s) 50, 110, 891
EcoO109I RGGNCCY 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 102, 321
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 2 cut(s) 350, 396
ErhI CCWWGG 2 cut(s) 350, 396
FaeI CATG 1 cut(s) 852
FatI CATG 1 cut(s) 848
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 38, 135, 146, 830, 888
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 5 cut(s) 38, 135, 146, 830, 888
FspBI CTAG 4 cut(s) 183, 191, 341, 351
GluI GCNGC 5 cut(s) 38, 135, 146, 830, 888
HaeIII GGCC 4 cut(s) 349, 471, 496, 656
HapII CCGG 1 cut(s) 415
Hin1II CATG 1 cut(s) 852
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 4 cut(s) 88, 359, 604, 893
HpaII CCGG 1 cut(s) 415
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 268, 877
Hpy188I TCNGA 6 cut(s) 69, 152, 513, 528, 550, 871
Hpy188III TCNNGA 4 cut(s) 8, 85, 341, 617
Hpy8I GTNNAC 2 cut(s) 268, 877
Hpy99I CGWCG 2 cut(s) 221, 413
HpyAV CCTTC 5 cut(s) 485, 516, 553, 667, 848
HpyCH4III ACNGT 4 cut(s) 221, 678, 777, 883
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 7 cut(s) 137, 268, 285, 299, 533, 579, 811
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 634
HpyF3I CTNAG 6 cut(s) 90, 170, 233, 527, 602, 738
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 852
Kzo9I GATC 3 cut(s) 751, 819, 823
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 4 cut(s) 23, 142, 153, 325
Lsp1109I GCAGC 5 cut(s) 24, 121, 157, 841, 899
LweI GCATC 1 cut(s) 541
MaeI CTAG 4 cut(s) 183, 191, 341, 351
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 753, 821, 825
MboI GATC 3 cut(s) 751, 819, 823
MboII GAAGA 6 cut(s) 88, 109, 206, 217, 683, 757
MhlI GDGCHC 3 cut(s) 28, 124, 270
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 7 cut(s) 107, 313, 333, 465, 565, 695, 786
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 3 cut(s) 82, 368, 613
MnlI CCTC 3 cut(s) 14, 282, 773
Mox20I TGGCCA 1 cut(s) 471
MroXI GAANNNNTTC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 471
MseI TTAA 6 cut(s) 116, 155, 260, 317, 800, 904
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 3 cut(s) 37, 134, 890
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 3 cut(s) 104, 323, 416
MvaI CCWGG 2 cut(s) 104, 323
MwoI GCNNNNNNNGC 2 cut(s) 539, 634
NciI CCSGG 1 cut(s) 416
NdeI CATATG 1 cut(s) 646
NdeII GATC 3 cut(s) 751, 819, 823
NlaIII CATG 1 cut(s) 852
NlaIV GGNNCC 2 cut(s) 621, 843
NspI RCATGY 1 cut(s) 852
PceI AGGCCT 2 cut(s) 349, 656
PciSI GCTCTTC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 893
PkrI GCNGC 5 cut(s) 39, 136, 147, 831, 889
PleI GAGTC 3 cut(s) 82, 367, 612
PpsI GAGTC 3 cut(s) 82, 367, 612
Ppu21I YACGTR 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 102, 321
PspGI CCWGG 2 cut(s) 102, 321
PspN4I GGNNCC 2 cut(s) 621, 843
PspPI GGNCC 2 cut(s) 393, 494
PstI CTGCAG 1 cut(s) 139
PstNI CAGNNNCTG 1 cut(s) 869
PvuII CAGCTG 2 cut(s) 37, 134
RsaI GTAC 1 cut(s) 710
RsaNI GTAC 1 cut(s) 709
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 6 cut(s) 116, 155, 260, 317, 800, 904
SatI GCNGC 5 cut(s) 38, 135, 146, 830, 888
Sau3AI GATC 3 cut(s) 751, 819, 823
Sau96I GGNCC 2 cut(s) 393, 494
SchI GAGTC 3 cut(s) 82, 368, 613
ScrFI CCNGG 3 cut(s) 104, 323, 416
SduI GDGCHC 3 cut(s) 28, 124, 270
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 2 cut(s) 135, 882
SinI GGWCC 1 cut(s) 393
Sse9I AATT 7 cut(s) 107, 313, 333, 465, 565, 695, 786
SseBI AGGCCT 2 cut(s) 349, 656
SsiI CCGC 2 cut(s) 255, 890
SspMI CTAG 4 cut(s) 183, 191, 341, 351
StuI AGGCCT 2 cut(s) 349, 656
StyD4I CCNGG 3 cut(s) 102, 321, 414
StyI CCWWGG 2 cut(s) 350, 396
TaaI ACNGT 4 cut(s) 221, 678, 777, 883
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 74, 822
TasI AATT 7 cut(s) 107, 313, 333, 465, 565, 695, 786
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 6 cut(s) 116, 155, 260, 317, 800, 904
Tru9I TTAA 6 cut(s) 116, 155, 260, 317, 800, 904
TscAI CASTG 3 cut(s) 144, 597, 886
TseI GCWGC 5 cut(s) 37, 134, 145, 829, 887
TspDTI ATGAA 1 cut(s) 227
TspRI CASTG 3 cut(s) 144, 597, 886
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 5 cut(s) 107, 313, 333, 565, 695
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 1 cut(s) 852
XmaJI CCTAGG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 4 cut(s) 183, 191, 341, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.