Rh2DG021500

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007544
Physical Location & Seq
Reverse (-)
1247738 .. 1248205
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG021500.1

Sequence Viewer

Length: 468 bp
ATGGCTCTTGATTTTTTGGGAGGAGCACCATTTCAGCTGCTTTATGATGGGGTGAAGCTCGCACTTCAGAACTCGAGGAGCTTCAAGACTCAGTTGAAGAACCTGGAATTCACTTTAAGAGCACTTCAGCCAGCAGTAGTGGAGCAGCTCCGATTAAACAACCTACATCTCAGCCTTTCTATTGGATACCTAGAAGAGTTAGAGAAGAAAATGAACGACGGTGTTCAGCTTCTTAGCCAAATGTCAAGTGTTAGCGGTTTAAATGTGCACTTATGGGGCTGTTGCGAGAGGAGTTATGCACAAGACCTTGAAAATTTAAACCAGGAGCTTCAAATTCTTCTAGAAAGGCTTAGGGTGGAGTCAATGATACTTACGAGACAGATTGCCATTTGGACCAGGGCACTATACGACGACCGGGATGATGGAACGGGAAAATGTATGAAGTATTGTTTAAATTCGACCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

155

Amino Acids

17.71

Weight (kDa)

5.45

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 114 3.3e-08 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 255
AcsI RAATTY 4 cut(s) 107, 313, 333, 454
AcuI CTGAAG 2 cut(s) 50, 110
AgsI TTSAA 4 cut(s) 85, 97, 311, 332
AjnI CCWGG 3 cut(s) 102, 321, 395
AluBI AGCT 6 cut(s) 37, 58, 81, 148, 229, 328
AluI AGCT 6 cut(s) 37, 58, 81, 148, 229, 328
Alw21I GWGCWC 3 cut(s) 28, 124, 270
Alw26I GTCTC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 73
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 2 cut(s) 37, 145
ApoI RAATTY 4 cut(s) 107, 313, 333, 454
AspS9I GGNCC 1 cut(s) 393
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 393
BaeGI GKGCMC 2 cut(s) 270, 403
Bbv12I GWGCWC 3 cut(s) 28, 124, 270
BbvI GCAGC 2 cut(s) 24, 157
BccI CCATC 2 cut(s) 41, 416
BciT130I CCWGG 3 cut(s) 104, 323, 397
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 2 cut(s) 191, 341
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 2 cut(s) 38, 146
BlsI GCNGC 2 cut(s) 39, 147
Bme1390I CCNGG 4 cut(s) 104, 323, 397, 416
Bme18I GGWCC 1 cut(s) 393
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 393
BmrFI CCNGG 4 cut(s) 104, 323, 397, 416
Bpu10I CCTNAGC 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 396
BseBI CCWGG 3 cut(s) 104, 323, 397
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 104, 184
BseRI GAGGAG 3 cut(s) 36, 91, 304
BseSI GKGCMC 2 cut(s) 270, 403
BseXI GCAGC 2 cut(s) 24, 157
Bsh1285I CGRYCG 2 cut(s) 415, 462
BsiEI CGRYCG 2 cut(s) 415, 462
BsiHKAI GWGCWC 3 cut(s) 28, 124, 270
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 4 cut(s) 28, 124, 270, 403
BspACI CCGC 1 cut(s) 255
BspCNI CTCAG 2 cut(s) 103, 183
BssECI CCNNGG 1 cut(s) 396
Bst2UI CCWGG 3 cut(s) 104, 323, 397
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 60, 132
BstDEI CTNAG 4 cut(s) 90, 170, 233, 350
BstF5I GGATG 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 370
BstMCI CGRYCG 2 cut(s) 415, 462
BstNI CCWGG 3 cut(s) 104, 323, 397
BstSCI CCNGG 4 cut(s) 102, 321, 395, 414
BstSLI GKGCMC 2 cut(s) 270, 403
BstV1I GCAGC 2 cut(s) 24, 157
BsuI GTATCC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 60, 132
Cfr13I GGNCC 1 cut(s) 393
DdeI CTNAG 4 cut(s) 90, 170, 233, 350
DraI TTTAAA 3 cut(s) 261, 318, 453
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 50, 110
Eco88I CYCGRG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 3 cut(s) 102, 321, 395
FaiI YATR 5 cut(s) 45, 274, 297, 406, 440
Fnu4HI GCNGC 2 cut(s) 38, 146
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 38, 146
FspBI CTAG 2 cut(s) 191, 341
GluI GCNGC 2 cut(s) 38, 146
HapII CCGG 1 cut(s) 415
HinfI GANTC 2 cut(s) 88, 359
HpaII CCGG 1 cut(s) 415
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 2 cut(s) 69, 152
Hpy188III TCNNGA 3 cut(s) 8, 85, 341
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 2 cut(s) 221, 413
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 2 cut(s) 268, 299
HpyF3I CTNAG 4 cut(s) 90, 170, 233, 350
LmnI GCTCC 5 cut(s) 23, 78, 142, 153, 325
LpnPI CCDG 8 cut(s) 89, 116, 144, 308, 335, 382, 409, 428
Lsp1109I GCAGC 2 cut(s) 24, 157
MaeI CTAG 2 cut(s) 191, 341
MboII GAAGA 4 cut(s) 109, 206, 217, 329
MhlI GDGCHC 4 cut(s) 28, 124, 270, 403
MluCI AATT 4 cut(s) 107, 313, 333, 454
MlyI GAGTC 2 cut(s) 82, 368
MnlI CCTC 3 cut(s) 14, 69, 282
MseI TTAA 5 cut(s) 116, 155, 260, 317, 452
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 4 cut(s) 104, 323, 397, 416
MvaI CCWGG 3 cut(s) 104, 323, 397
NciI CCSGG 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 73
PkrI GCNGC 2 cut(s) 39, 147
PleI GAGTC 2 cut(s) 82, 367
PpsI GAGTC 2 cut(s) 82, 367
Psp6I CCWGG 3 cut(s) 102, 321, 395
PspGI CCWGG 3 cut(s) 102, 321, 395
PspPI GGNCC 1 cut(s) 393
PspXI VCTCGAGB 1 cut(s) 73
PvuII CAGCTG 1 cut(s) 37
SaqAI TTAA 5 cut(s) 116, 155, 260, 317, 452
SatI GCNGC 2 cut(s) 38, 146
Sau96I GGNCC 1 cut(s) 393
SchI GAGTC 2 cut(s) 82, 368
ScrFI CCNGG 4 cut(s) 104, 323, 397, 416
SduI GDGCHC 4 cut(s) 28, 124, 270, 403
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 393
SlaI CTCGAG 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 4 cut(s) 107, 313, 333, 454
SsiI CCGC 1 cut(s) 255
SspMI CTAG 2 cut(s) 191, 341
StyD4I CCNGG 4 cut(s) 102, 321, 395, 414
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 2 cut(s) 74, 458
TasI AATT 4 cut(s) 107, 313, 333, 454
Tru1I TTAA 5 cut(s) 116, 155, 260, 317, 452
Tru9I TTAA 5 cut(s) 116, 155, 260, 317, 452
TseI GCWGC 2 cut(s) 37, 145
TspDTI ATGAA 2 cut(s) 227, 455
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 4 cut(s) 107, 313, 333, 454
XbaI TCTAGA 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 2 cut(s) 191, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.