Rh6DG317200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003925
Physical Location & Seq
Forward (+)
51950812 .. 51951435
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG317200.1

Sequence Viewer

Length: 624 bp
ATGGCAGCCCCGGAACCCAGCAACATCGACGAGGAGCTCTCCGCCGCCTCCACGACCATCTTATTCGACCGCCCCGTCCCCTTGCTCCGCGGGCCGGTCCGGGCCGGCCCGACCGACGACCCGTCGTCCGGCCCGTACGTGCTGGCATTCCGCGACGCGAAAACCTGGGCCAACGCCTACAGAGCGTGCGAGTCGAAGATCCTCCACCAGTGCGAGGCCGGGTCGAGAATCGGGTGCGCCCTCAGCGCATCGAGCAAGTGTAAGCCGCCGTGGTGGCGGTTTCTGATTGGGCAGAAAGCGCCGGAGTTGAAGCAGAGGGCGGAGTGCGAGGAGAGAGAGACGGAGGGGTGTTTGGCTACGGCGAAGGAGAAGTGCGTTGGGTTTGCGAGAGACAAGTGCTTGGAGCCGTTTAGGGAGGCCAGGGTTTGTGGGGTGAGTAAGAAACAGGCGGAGAAGATGGTGTGCTGGGCGAGTGTGATCGATAAAAGTAGTACATGGGTGAGTTTGATTGGCTTGGAGAATTGTGGGTTTGGGGCGACGAATTGTAGAGCGGTTGAGCTGGTTGGGTATTCCGGTGAGGTTGATCGGGTTTTAGGTGGTGGCAATGAGACTTTGAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.47

Weight (kDa)

7.41

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 551
AccII CGCG 3 cut(s) 90, 153, 158
AclWI GGATC 1 cut(s) 193
AfaI GTAC 2 cut(s) 137, 493
AfiI CCNNNNNNNGG 3 cut(s) 94, 128, 214
AgsI TTSAA 2 cut(s) 310, 616
AhdI GACNNNNNGTC 2 cut(s) 121, 124
AjnI CCWGG 2 cut(s) 164, 419
AluBI AGCT 2 cut(s) 37, 559
AluI AGCT 2 cut(s) 37, 559
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 3 cut(s) 332, 384, 602
AlwI GGATC 1 cut(s) 193
AoxI GGCC 7 cut(s) 92, 102, 106, 130, 168, 216, 417
ApeKI GCWGC 1 cut(s) 5
AspLEI GCGC 3 cut(s) 239, 248, 301
AspS9I GGNCC 6 cut(s) 92, 97, 102, 107, 131, 168
AsuC2I CCSGG 3 cut(s) 11, 101, 220
AsuHPI GGTGA 3 cut(s) 445, 511, 587
AvaII GGWCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 65, 451
BceAI ACGGC 3 cut(s) 253, 375, 391
BciT130I CCWGG 2 cut(s) 166, 421
BcnI CCSGG 3 cut(s) 11, 101, 220
BcoDI GTCTC 3 cut(s) 332, 384, 602
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 274
BisI GCNGC 3 cut(s) 6, 45, 266
BlsI GCNGC 3 cut(s) 7, 46, 267
Bme1390I CCNGG 5 cut(s) 11, 101, 166, 220, 421
Bme18I GGWCC 1 cut(s) 97
BmeRI GACNNNNNGTC 2 cut(s) 121, 124
BmgT120I GGNCC 6 cut(s) 92, 97, 102, 107, 131, 168
BmiI GGNNCC 2 cut(s) 15, 405
BmrFI CCNGG 5 cut(s) 11, 101, 166, 220, 421
BmsI GCATC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu10I CCTNAGC 1 cut(s) 242
BpuMI CCSGG 3 cut(s) 11, 101, 220
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 139
BsaJI CCNNGG 5 cut(s) 9, 88, 165, 269, 420
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 128, 214
Bse118I RCCGGY 2 cut(s) 94, 104
Bse1I ACTGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 610
BseBI CCWGG 2 cut(s) 166, 421
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 5 cut(s) 9, 88, 165, 269, 420
BseLI CCNNNNNNNGG 3 cut(s) 94, 128, 214
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 2 cut(s) 47, 344
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 2 cut(s) 17, 465
Bsh1236I CGCG 3 cut(s) 90, 153, 158
Bsh1285I CGRYCG 2 cut(s) 70, 114
BshFI GGCC 7 cut(s) 94, 104, 108, 132, 170, 218, 419
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 2 cut(s) 70, 114
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 8 cut(s) 11, 95, 100, 105, 129, 219, 302, 573
BsiWI CGTACG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 94, 128, 214
BsmAI GTCTC 3 cut(s) 332, 384, 602
BsmBI CGTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 7 cut(s) 94, 104, 108, 132, 170, 218, 419
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 198, 477, 583
BspANI GGCC 7 cut(s) 94, 104, 108, 132, 170, 218, 419
BspCNI CTCAG 1 cut(s) 255
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 3 cut(s) 90, 153, 158
BspLI GGNNCC 2 cut(s) 15, 405
BspPI GGATC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 2 cut(s) 94, 104
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 2 cut(s) 94, 104
BssECI CCNNGG 5 cut(s) 9, 88, 165, 269, 420
BssMI GATC 3 cut(s) 198, 477, 583
Bst2UI CCWGG 2 cut(s) 166, 421
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 4 cut(s) 92, 106, 144, 187
BstDEI CTNAG 1 cut(s) 242
BstDSI CCRYGG 2 cut(s) 88, 269
BstFNI CGCG 3 cut(s) 90, 153, 158
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 3 cut(s) 239, 248, 301
BstKTI GATC 3 cut(s) 201, 480, 586
BstMAI GTCTC 3 cut(s) 332, 384, 602
BstMBI GATC 3 cut(s) 198, 477, 583
BstMCI CGRYCG 2 cut(s) 70, 114
BstMWI GCNNNNNNNGC 7 cut(s) 91, 182, 243, 245, 252, 274, 298
BstNI CCWGG 2 cut(s) 166, 421
BstSCI CCNGG 5 cut(s) 9, 99, 164, 218, 419
BstSFI CTRYAG 1 cut(s) 178
BstUI CGCG 3 cut(s) 90, 153, 158
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 7 cut(s) 94, 104, 108, 132, 170, 218, 419
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 2 cut(s) 88, 269
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 4 cut(s) 92, 106, 144, 187
CfoI GCGC 3 cut(s) 239, 248, 301
Cfr10I RCCGGY 2 cut(s) 94, 104
Cfr13I GGNCC 6 cut(s) 92, 97, 102, 107, 131, 168
Cfr42I CCGCGG 1 cut(s) 91
ClaI ATCGAT 1 cut(s) 480
CpoI CGGWCCG 1 cut(s) 97
CseI GACGC 1 cut(s) 164
Csp6I GTAC 2 cut(s) 136, 492
CspI CGGWCCG 1 cut(s) 97
CviAII CATG 1 cut(s) 495
CviQI GTAC 2 cut(s) 136, 492
DdeI CTNAG 1 cut(s) 242
DpnI GATC 3 cut(s) 200, 479, 585
DpnII GATC 3 cut(s) 198, 477, 583
DrdI GACNNNNNNGTC 1 cut(s) 74
DriI GACNNNNNGTC 2 cut(s) 121, 124
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1105I GACNNNNNGTC 2 cut(s) 121, 124
EciI GGCGGA 3 cut(s) 31, 335, 464
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 164, 419
EcoT38I GRGCYC 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 1 cut(s) 498
FaiI YATR 1 cut(s) 496
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 494
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 3 cut(s) 6, 45, 266
FriOI GRGCYC 1 cut(s) 39
FseI GGCCGGCC 1 cut(s) 108
Fsp4HI GCNGC 3 cut(s) 6, 45, 266
GlaI GCGC 3 cut(s) 238, 247, 300
GluI GCNGC 3 cut(s) 6, 45, 266
GsaI CCCAGC 2 cut(s) 21, 469
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 7 cut(s) 94, 104, 108, 132, 170, 218, 419
HapII CCGG 8 cut(s) 11, 95, 100, 105, 129, 219, 302, 573
HgaI GACGC 1 cut(s) 164
HhaI GCGC 3 cut(s) 239, 248, 301
Hin1II CATG 1 cut(s) 498
Hin6I GCGC 3 cut(s) 237, 246, 299
HinP1I GCGC 3 cut(s) 237, 246, 299
HinfI GANTC 2 cut(s) 191, 228
HpaII CCGG 8 cut(s) 11, 95, 100, 105, 129, 219, 302, 573
HphI GGTGA 3 cut(s) 445, 511, 587
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 1 cut(s) 225
Hpy99I CGWCG 5 cut(s) 32, 119, 127, 158, 541
HpyAV CCTTC 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 182, 243, 245, 252, 274, 298
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 1 cut(s) 498
HspAI GCGC 3 cut(s) 237, 246, 299
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 3 cut(s) 198, 477, 583
LmnI GCTCC 3 cut(s) 34, 90, 403
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 257
MaeII ACGT 1 cut(s) 138
MalI GATC 3 cut(s) 200, 479, 585
MbiI CCGCTC 1 cut(s) 551
MboI GATC 3 cut(s) 198, 477, 583
MboII GAAGA 2 cut(s) 208, 466
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 2 cut(s) 520, 541
MlyI GAGTC 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 8 cut(s) 11, 95, 100, 105, 129, 219, 302, 573
MspR9I CCNGG 5 cut(s) 11, 101, 166, 220, 421
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 2 cut(s) 166, 421
MvnI CGCG 3 cut(s) 90, 153, 158
MwoI GCNNNNNNNGC 7 cut(s) 91, 182, 243, 245, 252, 274, 298
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 3 cut(s) 11, 101, 220
NdeII GATC 3 cut(s) 198, 477, 583
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 1 cut(s) 498
NlaIV GGNNCC 2 cut(s) 15, 405
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 131
PctI GAATGC 1 cut(s) 146
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 228
Pfl23II CGTACG 1 cut(s) 135
PkrI GCNGC 3 cut(s) 7, 46, 267
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 164, 419
PspFI CCCAGC 2 cut(s) 17, 465
PspGI CCWGG 2 cut(s) 164, 419
PspLI CGTACG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 15, 405
PspPI GGNCC 6 cut(s) 92, 97, 102, 107, 131, 168
PsuI RGATCY 1 cut(s) 198
RigI GGCCGGCC 1 cut(s) 108
RsaI GTAC 2 cut(s) 137, 493
RsaNI GTAC 2 cut(s) 136, 492
Rsr2I CGGWCCG 1 cut(s) 97
RsrII CGGWCCG 1 cut(s) 97
SacI GAGCTC 1 cut(s) 39
SacII CCGCGG 1 cut(s) 91
SatI GCNGC 3 cut(s) 6, 45, 266
Sau3AI GATC 3 cut(s) 198, 477, 583
Sau96I GGNCC 6 cut(s) 92, 97, 102, 107, 131, 168
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 5 cut(s) 11, 101, 166, 220, 421
SduI GDGCHC 1 cut(s) 39
SetI ASST 6 cut(s) 39, 141, 167, 561, 582, 598
SfaNI GCATC 1 cut(s) 257
SfcI CTRYAG 1 cut(s) 178
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
SinI GGWCC 1 cut(s) 97
Sse9I AATT 2 cut(s) 520, 541
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 9, 99, 164, 218, 419
TaiI ACGT 1 cut(s) 141
TaqI TCGA 6 cut(s) 27, 66, 194, 224, 251, 480
TaqII GACCGA 1 cut(s) 128
TasI AATT 2 cut(s) 520, 541
TatI WGTACW 1 cut(s) 491
TauI GCSGC 2 cut(s) 47, 268
TfiI GAWTC 1 cut(s) 228
TscAI CASTG 1 cut(s) 215
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.