Rw2G018340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003925
Physical Location & Seq
Forward (+)
23495598 .. 23495963
366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018340.1

Sequence Viewer

Length: 366 bp
ATGGACGCCACTGTCTCCCCAGACCCAATCCCCAACCCGAATCCGTCGTCCGACCCATACGTGCTGGCATTCAGTGACCCGAAAACCTGGGCCAAAGCCTTCAGAGAGTGCGAGTCCAATATCCTACACCAATGCGAGGTCGGATCGAGAATCGGGTGCGCCATCATCACATCGAGCAAGTGTAAGCCGCCGTGGTGGCGGTTTCTGATTGGGCAAAAAGCACCGGACTTGAAGCAGAGGACGGAGTGCGAGGAGAGAGAGACGGAGGGGTGTTTGGCTCCGAATTGTAGGGCGGGTGGGCTGGTTGGTTGTTCCGGTGTGGTTGATCGGGTTTTAGTTGGTGGCAGTGAGACTTTGAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.13

Weight (kDa)

5.82

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AciI CCGC 3 cut(s) 188, 199, 293
AclWI GGATC 1 cut(s) 151
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 136
AgsI TTSAA 2 cut(s) 232, 358
AjnI CCWGG 1 cut(s) 86
Alw26I GTCTC 3 cut(s) 19, 254, 344
AlwI GGATC 1 cut(s) 151
AoxI GGCC 1 cut(s) 90
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 90
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 1 cut(s) 88
BcoDI GTCTC 3 cut(s) 19, 254, 344
BglI GCCNNNNNGGC 1 cut(s) 196
BisI GCNGC 1 cut(s) 188
BlsI GCNGC 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 87, 191
BsaWI WCCGGW 2 cut(s) 223, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 136
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 2 cut(s) 87, 191
BseLI CCNNNNNNNGG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 266
BshFI GGCC 1 cut(s) 92
BsiSI CCGG 2 cut(s) 224, 315
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 3 cut(s) 19, 254, 344
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 143, 325
BspACI CCGC 3 cut(s) 188, 199, 293
BspANI GGCC 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 279
BspPI GGATC 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 87, 191
BssMI GATC 2 cut(s) 143, 325
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 1 cut(s) 13
BstACI GRCGYC 1 cut(s) 6
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 66
BstDSI CCRYGG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 146, 328
BstMAI GTCTC 3 cut(s) 19, 254, 344
BstMBI GATC 2 cut(s) 143, 325
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 86
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 191
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 9, 79, 352
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 90
CseI GACGC 1 cut(s) 14
CviJI RGCY 5 cut(s) 92, 98, 187, 278, 301
CviKI_1 RGCY 5 cut(s) 92, 98, 187, 278, 301
DpnI GATC 2 cut(s) 145, 327
DpnII GATC 2 cut(s) 143, 325
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 254
FaiI YATR 1 cut(s) 58
FauI CCCGC 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 188
GlaI GCGC 1 cut(s) 160
GluI GCNGC 1 cut(s) 188
HaeIII GGCC 1 cut(s) 92
HapII CCGG 2 cut(s) 224, 315
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 3 cut(s) 40, 113, 150
HpaII CCGG 2 cut(s) 224, 315
Hpy188I TCNGA 5 cut(s) 52, 104, 143, 207, 282
Hpy188III TCNNGA 1 cut(s) 147
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 143, 325
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 7 cut(s) 33, 50, 73, 100, 237, 287, 328
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 74
MalI GATC 2 cut(s) 145, 327
MboI GATC 2 cut(s) 143, 325
MluCI AATT 1 cut(s) 283
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 75, 121
MnlI CCTC 4 cut(s) 130, 231, 244, 259
MspI CCGG 2 cut(s) 224, 315
MspR9I CCNGG 1 cut(s) 88
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 196
NdeII GATC 2 cut(s) 143, 325
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 74
PctI GAATGC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 40, 150
PkrI GCNGC 1 cut(s) 189
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 90
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 2 cut(s) 143, 325
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 88
SetI ASST 3 cut(s) 63, 89, 141
Sse9I AATT 1 cut(s) 283
SsiI CCGC 3 cut(s) 188, 199, 293
StyD4I CCNGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 63
TaqI TCGA 2 cut(s) 146, 173
TasI AATT 1 cut(s) 283
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 2 cut(s) 40, 150
TscAI CASTG 3 cut(s) 16, 79, 352
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
TspGWI ACGGA 3 cut(s) 33, 257, 278
TspRI CASTG 3 cut(s) 16, 79, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.