Rw3G014010

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003118
Physical Location & Seq
Reverse (-)
13780323 .. 13788178
7856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014010.1

Sequence Viewer

Length: 699 bp
ATGGAGAAAATTGCCTGGCTGATATTGGTGTTCTTGATTGGCAAATGTTACATCAACTCATCAGAGATAAAAGTTTTGCAGATTGACCCGGAAAAATACAAAAGCGTTATCTCTGGATTTGGAGTTACGTTCAGGGAGCAAGGAATTAGAGGTTTCTTTAGGGGTTGGGTGCCCACTTTGATTGGTTACAGTGCTCAGGGTGCCGGCAAGTACGGCCTCTACGAGTTCTTTGCCGTTTGCCAGAGGTTTGTCAGATGGGCTACCAAATACAAAGGGGTTGTTCCTCTTTGGGAACGCCAAATTCCTTATGCAATGATGAAATTTTCAACTTTCGAGAAATTTGTCGAGCTCACTTACAAGCATGTGATCCCAACACCGAAAGAGCAGTGCAGCACACCCTTGCAGCTCGGAGTGAGCTTTGCGTGTGGATATGTGGCTGGTGTGTTCTGTGCTGTTGTTTCACACCCTGCTGATAACTTGGTGTCTTTCCTCAACAATGCCAAGGGGGGAACTGTTAGTGATGCTGTGAAGAATCTAGGAGTGTGGGGTCTTTTCCCCCGGGGTCTTCCTCATCGTATAGTCATGATCGGAACCCTTACTGGAAGCCAATGGGGTATCTATGATGCCTGCAAAGTTTTCATGGGCGTACCAACTACTGGCGGCGCTGCTCCTGCTGCCACTGAGCTTGCCAATGCTTGA

Protein Analysis

232

Amino Acids

25.53

Weight (kDa)

9.29

Isoelectric Point (pI)

27.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 26 - 77 1.8e-09 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 169, 200
AccB7I CCANNNNNTGG 1 cut(s) 656
AciI CCGC 1 cut(s) 660
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 3 cut(s) 300, 320, 338
AfaI GTAC 2 cut(s) 212, 648
AfiI CCNNNNNNNGG 1 cut(s) 656
AgsI TTSAA 1 cut(s) 327
AjnI CCWGG 1 cut(s) 14
AluBI AGCT 4 cut(s) 349, 406, 417, 685
AluI AGCT 4 cut(s) 349, 406, 417, 685
Alw21I GWGCWC 2 cut(s) 196, 351
AlwI GGATC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 4 cut(s) 390, 403, 665, 674
ApoI RAATTY 3 cut(s) 300, 320, 338
AspLEI GCGC 1 cut(s) 665
AsuC2I CCSGG 3 cut(s) 89, 559, 560
AvaI CYCGRG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 2 cut(s) 169, 200
BanII GRGCYC 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 2 cut(s) 196, 351
BbvI GCAGC 4 cut(s) 402, 415, 652, 661
BccI CCATC 1 cut(s) 249
BceAI ACGGC 2 cut(s) 218, 229
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 16
BcnI CCSGG 3 cut(s) 89, 559, 560
BfaI CTAG 1 cut(s) 536
BfoI RGCGCY 1 cut(s) 666
BisI GCNGC 5 cut(s) 391, 404, 661, 666, 675
BlsI GCNGC 5 cut(s) 392, 405, 662, 667, 676
Bme1390I CCNGG 4 cut(s) 16, 89, 559, 560
BmeT110I CYCGRG 1 cut(s) 558
BmiI GGNNCC 3 cut(s) 171, 202, 592
BmrFI CCNGG 4 cut(s) 16, 89, 559, 560
BmsI GCATC 2 cut(s) 511, 613
BpiI GAAGAC 1 cut(s) 557
Bpu10I CCTNAGC 1 cut(s) 195
BpuMI CCSGG 3 cut(s) 89, 559, 560
BsaJI CCNNGG 4 cut(s) 501, 557, 558, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 604, 661
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 16
BseDI CCNNGG 4 cut(s) 501, 557, 558, 559
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 209, 672
BseNI ACTGG 2 cut(s) 604, 661
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 4 cut(s) 402, 415, 652, 661
BsgI GTGCAG 1 cut(s) 409
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 2 cut(s) 169, 200
BsiHKAI GWGCWC 2 cut(s) 196, 351
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 3 cut(s) 89, 204, 559
BslI CCNNNNNNNGG 1 cut(s) 656
BsnI GGCC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 174, 196, 351
Bsp143I GATC 2 cut(s) 366, 585
BspACI CCGC 1 cut(s) 660
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 2 cut(s) 208, 673
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 3 cut(s) 171, 202, 592
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 2 cut(s) 169, 200
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 604, 661
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 4 cut(s) 501, 557, 558, 559
BssMI GATC 2 cut(s) 366, 585
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 2 cut(s) 191, 514
BstC8I GCNNGC 3 cut(s) 205, 628, 687
BstDEI CTNAG 2 cut(s) 195, 681
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 2 cut(s) 369, 588
BstMBI GATC 2 cut(s) 366, 585
BstMWI GCNNNNNNNGC 4 cut(s) 200, 213, 671, 674
BstNI CCWGG 1 cut(s) 16
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 4 cut(s) 14, 87, 557, 558
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 4 cut(s) 402, 415, 652, 661
BstV2I GAAGAC 1 cut(s) 557
BsuRI GGCC 1 cut(s) 216
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 3 cut(s) 196, 392, 678
Cac8I GCNNGC 3 cut(s) 205, 628, 687
CciI TCATGA 1 cut(s) 582
CfoI GCGC 1 cut(s) 665
Cfr10I RCCGGY 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 558
Csp6I GTAC 2 cut(s) 211, 647
CspCI CAANNNNNGTGG 3 cut(s) 163, 198, 667
CviAII CATG 3 cut(s) 362, 583, 640
CviJI RGCY 9 cut(s) 19, 216, 260, 349, 406, 417, 437, 606, 685
CviKI_1 RGCY 9 cut(s) 19, 216, 260, 349, 406, 417, 437, 606, 685
CviQI GTAC 2 cut(s) 211, 647
DdeI CTNAG 2 cut(s) 195, 681
DpnI GATC 2 cut(s) 368, 587
DpnII GATC 2 cut(s) 366, 585
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 558
EcoICRI GAGCTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 3 cut(s) 365, 586, 643
FaiI YATR 7 cut(s) 309, 363, 432, 578, 584, 621, 641
FatI CATG 3 cut(s) 361, 582, 639
Fnu4HI GCNGC 5 cut(s) 391, 404, 661, 666, 675
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 5 cut(s) 391, 404, 661, 666, 675
FspBI CTAG 1 cut(s) 536
GlaI GCGC 1 cut(s) 664
GluI GCNGC 5 cut(s) 391, 404, 661, 666, 675
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 1 cut(s) 216
HapII CCGG 3 cut(s) 89, 204, 559
HhaI GCGC 1 cut(s) 665
Hin1II CATG 3 cut(s) 365, 586, 643
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HinfI GANTC 1 cut(s) 532
HpaII CCGG 3 cut(s) 89, 204, 559
Hpy188I TCNGA 4 cut(s) 64, 254, 410, 590
Hpy188III TCNNGA 4 cut(s) 34, 114, 334, 583
HpyCH4III ACNGT 2 cut(s) 191, 514
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 5 cut(s) 79, 311, 390, 403, 630
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 213, 671, 674
HpyF3I CTNAG 2 cut(s) 195, 681
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 3 cut(s) 365, 586, 643
HspAI GCGC 1 cut(s) 663
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 2 cut(s) 366, 585
LmnI GCTCC 2 cut(s) 136, 673
Lsp1109I GCAGC 4 cut(s) 402, 415, 652, 661
LweI GCATC 2 cut(s) 511, 613
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 3 cut(s) 47, 124, 185
MalI GATC 2 cut(s) 368, 587
MboI GATC 2 cut(s) 366, 585
MboII GAAGA 2 cut(s) 541, 557
MhlI GDGCHC 3 cut(s) 174, 196, 351
MluCI AATT 5 cut(s) 9, 144, 300, 320, 338
MnlI CCTC 6 cut(s) 143, 227, 237, 294, 500, 579
MroNI GCCGGC 1 cut(s) 203
MspI CCGG 3 cut(s) 89, 204, 559
MspR9I CCNGG 4 cut(s) 16, 89, 559, 560
MvaI CCWGG 1 cut(s) 16
MwoI GCNNNNNNNGC 4 cut(s) 200, 213, 671, 674
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 3 cut(s) 89, 559, 560
NdeII GATC 2 cut(s) 366, 585
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 3 cut(s) 365, 586, 643
NlaIV GGNNCC 3 cut(s) 171, 202, 592
NspI RCATGY 1 cut(s) 365
PagI TCATGA 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 532
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 5 cut(s) 392, 405, 662, 667, 676
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 3 cut(s) 171, 202, 592
RsaI GTAC 2 cut(s) 212, 648
RsaNI GTAC 2 cut(s) 211, 647
SacI GAGCTC 1 cut(s) 351
SatI GCNGC 5 cut(s) 391, 404, 661, 666, 675
Sau3AI GATC 2 cut(s) 366, 585
ScrFI CCNGG 4 cut(s) 16, 89, 559, 560
SduI GDGCHC 3 cut(s) 174, 196, 351
SetI ASST 7 cut(s) 131, 154, 248, 351, 408, 419, 687
SfaNI GCATC 2 cut(s) 511, 613
SmaI CCCGGG 1 cut(s) 560
Sse9I AATT 5 cut(s) 9, 144, 300, 320, 338
SsiI CCGC 1 cut(s) 660
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 4 cut(s) 14, 87, 557, 558
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 191, 514
TaiI ACGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 333, 345
TasI AATT 5 cut(s) 9, 144, 300, 320, 338
TauI GCSGC 1 cut(s) 663
TfiI GAWTC 1 cut(s) 532
TscAI CASTG 3 cut(s) 196, 392, 685
TseI GCWGC 4 cut(s) 390, 403, 665, 674
TspDTI ATGAA 2 cut(s) 332, 628
TspMI CCCGGG 1 cut(s) 558
TspRI CASTG 3 cut(s) 196, 392, 685
Van91I CCANNNNNTGG 1 cut(s) 656
XapI RAATTY 3 cut(s) 300, 320, 338
XceI RCATGY 1 cut(s) 365
XmaI CCCGGG 1 cut(s) 558
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.