AT1G05570

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002059
Physical Location & Seq
Reverse (-)
1647575 .. 1659611
12037 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05570.2

Sequence Viewer

Length: 5853 bp
ATGGCTCAAAGAAGGGAACCTGATCCGCCTCCACCGCAGAGGCGGATTCTGCGGACTCAGACTGTTGGTAGTCTCGGAGAGGCTATGCTTGATAGTGAAGTTGTTCCCTCTTCTTTAGTTGAGATTGCTCCGATTCTACGTGTGGCTAATGAAGTTGAAGCTAGTAATCCACGAGTTGCTTACCTATGTCGTTTCTACGCATTTGAGAAGGCGCATAGGCTTGATCCAACTTCAAGTGGTCGTGGTGTTCGCCAGTTTAAGACCGCGCTTCTTCAACGATTAGAAAGGGAGAATGAAACAACTTTGGCAGGAAGGCAAAAGAGTGATGCACGTGAAATGCAGAGTTTCTATCAACACTACTACAAGAAATACATCCAAGCTTTGCTGAATGCTGCGGATAAAGCTGACCGTGCTCAACTTACAAAGGCCTATCAAACTGCTGCCGTTCTCTTTGAAGTCTTGAAGGCTGTTAATCAAACAGAAGATGTGGAAGTGGCTGATGAGATTTTGGAAACGCACAATAAGGTGGAAGAGAAGACCCAGATTTACGTGCCTTACAATATACTTCCCCTTGACCCTGATAGTCAGAATCAGGCAATAATGAGATTGCCTGAGATACAAGCTGCTGTAGCCGCTCTTAGAAATACAAGGGGATTACCATGGACAGCAGGTCACAAGAAGAAACTCGATGAAGACATTCTAGACTGGCTTCAGTCTATGTTTGGTTTTCAGAAAGATAATGTTTTGAATCAAAGAGAGCACTTGATCTTGTTACTTGCTAATGTCCACATCCGACAATTTCCAAAACCTGACCAACAACCAAAGTTGGATGATCGTGCCTTGACCATAGTGATGAAGAAACTTTTCAGAAACTACAAAAAGTGGTGCAAATACTTGGGTCGGAAAAGCAGCCTTTGGTTGCCTACTATTCAGCAAGAGGTACAGCAGCGTAAACTGCTCTACATGGGACTTTATCTATTAATATGGGGAGAAGCTGCAAACCTGAGATTCATGCCAGAATGCCTCTGTTATATATATCACCATATGGCTTTCGAGCTTTATGGTATGTTGGCCGGTAGTGTTAGTCCCATGACAGGGGAGCATGTAAAGCCAGCGTATGGTGGTGAAGATGAAGCTTTCCTGCAGAAAGTTGTGACTCCTATATACCAGACAATATCAAAGGAAGCAAAGAGAAGTAGAGGAGGAAAGTCAAAGCATTCTGTGTGGAGAAACTATGATGACTTGAATGAATATTTTTGGTCAATTCGATGTTTTCGCTTAGGGTGGCCAATGCGGGCTGATGCTGATTTCTTTTGTCAGACAGCTGAAGAACTTCGACTTGAGAGAAGTGAGATAAAATCAAACAGTGGAGATCGGTGGATGGGCAAAGTCAATTTTGTGGAGATACGCTCGTTCTGGCATATATTTAGAAGTTTTGATAGATTGTGGAGCTTTTATATCCTGTGTTTGCAGGCAATGATTGTTATTGCATGGAATGGATCCGGAGAGTTAAGCGCCATTTTTCAGGGTGATGTCTTCCTAAAGGTTTTGAGCGTCTTCATTACAGCAGCTATACTAAAGCTTGCACAAGCTGTCCTTGACATAGCCCTCAGCTGGAAGGCAAGACATAGTATGTCACTCTATGTGAAACTCAGATACGTCATGAAGGTAGGTGCGGCTGCAGTATGGGTTGTAGTCATGGCAGTTACTTATGCGTACAGCTGGAAAAACGCCTCCGGTTTTTCTCAGACCATAAAGAATTGGTTTGGGGGACACAGCCATAATTCACCTTCTCTTTTCATAGTGGCAATTCTTATCTACTTATCCCCGAACATGCTTTCTGCGTTGCTGTTTTTGTTTCCATTCATTCGGCGCTATCTTGAAAGGTCTGACTATAAGATAATGATGCTGATGATGTGGTGGTCTCAGCCTCGTCTGTATATAGGAAGGGGTATGCATGAGAGCGCTTTGTCACTTTTCAAGTACACAATGTTTTGGATCGTTCTGCTGATTTCAAAGCTGGCATTCAGTTATTACGCTGAGATTAAGCCCCTTGTTGGTCCAACCAAAGATATAATGCGAATCCATATATCCGTCTACAGCTGGCATGAGTTTTTCCCTCACGCCAAGAATAATTTAGGAGTCGTCATCGCGCTCTGGTCACCAGTTATTCTCGTTTATTTCATGGACACTCAAATATGGTATGCTATAGTTTCAACTCTGGTCGGAGGTTTAAATGGAGCTTTCCGCCGTCTTGGAGAGATCCGAACACTTGGAATGTTGAGGTCGAGATTTCAGTCCATACCAGGGGCCTTTAATGATTGCTTGGTACCACAAGATAACAGCGATGATACAAAGAAAAAGAGATTTAGGGCGACCTTCTCTCGCAAGTTTGATCAGCTCCCATCTAGTAAAGACAAGGAAGCAGCGCGGTTTGCTCAGATGTGGAACAAAATAATCAGTAGTTTCCGAGAAGAGGACTTGATTAGTGATAGGGAAATGGAACTATTGCTTGTGCCATATTGGTCTGACCCTGATTTGGATCTTATCCGGTGGCCACCTTTTCTATTGGCTAGTAAGATCCCAATAGCTTTGGATATGGCTAAAGACAGCAATGGAAAAGACCGTGAACTGAAAAAGAGATTAGCTGTTGACAGCTACATGACTTGTGCAGTTAGAGAGTGCTATGCCTCTTTTAAGAATCTCATCAATTATTTGGTGGTCGGAGAACGAGAAGGACAGGTCATAAACGACATTTTCTCCAAGATTGATGAACATATTGAGAAGGAAACGCTGATAACAGAATTGAACCTGAGTGCTCTTCCTGATCTGTATGGCCAGTTCGTTCGGCTAATCGAATATTTGCTAGAAAACAGGGAGGAGGACAAGGATCAAATTGTAATTGTCTTGCTGAACATGTTAGAATTGGTGACTAGAGACATCATGGAAGAAGAAGTCCCAAGCTTACTAGAGACTGCCCACAATGGATCTTATGTCAAGTATGACGTCATGACCCCTCTTCATCAACAGCGCAAATATTTCAGTCAACTTCGATTCCCAGTTTATTCTCAAACGGAAGCTTGGAAAGAAAAAATTAAGAGGCTTCATCTTTTGCTTACTGTCAAGGAGTCAGCTATGGATGTTCCATCCAACTTGGAAGCTCGAAGGCGTCTTACTTTCTTTTCAAATTCACTATTCATGGACATGCCTCCTGCACCCAAGATCCGCAACATGCTTTCCTTCTCGGTCCTTACACCATACTTTTCAGAGGATGTTCTTTTCTCCATATTTGGCTTAGAACAGCAAAATGAAGATGGAGTTTCTATACTCTTTTACTTGCAGAAGATCTTCCCAGATGAATGGACAAACTTTCTGGAACGAGTTAAATGTGGCAACGAAGAAGAACTAAGAGCAAGAGAGGATTTGGAAGAGGAGCTTCGTCTTTGGGCATCTTACAGAGGCCAAACTCTGACTAAAACTGTGCGAGGAATGATGTATTACCGAAAGGCTTTGGAACTTCAGGCCTTCCTTGATATGGCAAAGGATGAAGAATTATTAAAAGGCTACAAGGCTCTCGAGTTAACCAGTGAAGAAGCATCAAAGAGCGGAGGGTCACTTTGGGCGCAGTGTCAAGCACTTGCAGATATGAAGTTCACCTTTGTGGTTTCTTGTCAACAATACAGTATTCATAAGAGATCTGGCGACCAACGTGCTAAAGACATATTGAGACTTATGACGACGTATCCGTCTATTCGTGTCGCCTATATCGATGAGGTAGAGCAAACACATAAAGAAAGTTATAAGGGGACAGAGGAGAAAATTTATTACTCAGCGCTTGTAAAAGCTGCTCCACAGACCAAACCAATGGATTCTTCTGAATCTGTTCAAACACTAGATCAGCTCATTTATCGGATTAAGCTTCCGGGACCAGCTATACTAGGAGAGGGAAAGCCGGAAAATCAGAACCATGCTATCATATTTACCCGTGGAGAAGGGTTGCAGACGATAGACATGAATCAGGACAACTACATGGAGGAAGCTTTCAAAATGAGGAACTTGCTGCAAGAGTTTCTTGAAAAGCATGGAGGCGTAAGATGTCCTACGATTCTTGGTCTTAGAGAGCATATTTTCACTGGAAGTGTGTCTTCTCTTGCATGGTTTATGTCAAATCAAGAGAACAGTTTTGTAACGATTGGGCAAAGAGTGCTAGCTAGTCCCTTGAAAGTACGATTCCATTACGGACATCCAGATATTTTTGATCGTCTGTTTCACCTTACCAGAGGTGGTATCTGCAAAGCTTCTAAAGTCATCAATCTTAGTGAAGACATATTTGCAGGTTTTAATTCTACTCTGCGTGAAGGAAATGTGACTCATCATGAATATATACAAGTTGGTAAAGGGAGAGATGTGGGCCTCAACCAGATCTCAATGTTTGAGGCAAAAATCGCCAATGGAAATGGCGAGCAAACTTTGAGTCGCGACCTTTATAGGCTAGGACACCGATTTGATTTCTTCCGGATGCTGTCTTGTTATTTCACCACAATTGGGTTCTACTTCAGTACCATGTTAACCGTGCTTACGGTGTATGTGTTTCTTTATGGACGGTTATATCTAGTACTTAGTGGGCTTGAAGAAGGATTGAGTAGCCAACGAGCTTTCCGTAACAATAAGCCTCTTGAGGCAGCTCTTGCTTCTCAATCATTTGTGCAGATTGGTTTTTTAATGGCTTTGCCTATGATGATGGAGATTGGACTTGAGAGGGGGTTCCACAATGCATTGATTGAGTTTGTGCTGATGCAGTTACAACTTGCTTCCGTCTTTTTCACGTTTCAGCTCGGGACAAAAACACATTACTACGGAAGGACATTGTTTCATGGAGGCGCAGAGTATAGAGGAACTGGTCGAGGGTTTGTGGTCTTCCACGCGAAATTTGCTGAGAACTATAGGTTCTATTCCCGTAGTCACTTTGTGAAGGGCATTGAACTTATGATTCTTCTACTGGTATATCAGATCTTTGGTCAATCATATAGGGGTGTGGTTACTTATATTCTGATCACTGTTTCGATATGGTTCATGGTGGTGACATGGCTCTTTGCTCCTTTCCTTTTCAATCCGTCTGGTTTTGAGTGGCAAAAGATTGTTGATGACTGGACAGACTGGAACAAATGGATTTATAACCGAGGAGGAATTGGTGTCCCTCCTGAGAAGAGTTGGGAATCTTGGTGGGAGAAAGAGTTAGAACATCTTAGACATTCAGGGGTTCGTGGTATCACCCTTGAGATCTTTTTGGCCTTGCGCTTCTTCATCTTTCAATATGGACTTGTGTATCATCTCAGTACCTTCAAAGGAAAAAATCAGAGCTTTTGGGTTTATGGAGCGTCATGGTTTGTGATCTTATTCATTCTACTGATTGTGAAGGGTTTGGGCGTTGGAAGGCGAAGATTCAGCACGAATTTCCAGCTTCTATTCAGAATCATAAAGGGTCTTGTGTTCCTCACATTTGTGGCAATTCTCATAACTTTCCTCGCACTTCCTCTGATAACAATCAAAGACTTATTCATCTGCATGCTTGCCTTCATGCCAACTGGATGGGGAATGCTTCTCATTGCACAAGCGTGTAAGCCTCTGATCCAACAGCTAGGGATATGGTCATCAGTTAGGACGCTAGCTCGAGGCTACGAGATCGTGATGGGCTTGCTTCTGTTCACACCAGTTGCGTTTTTGGCCTGGTTTCCTTTTGTGTCTGAGTTCCAGACAAGAATGCTCTTTAACCAAGCCTTTAGTAGAGGTCTACAAATCTCTCGTATTCTTGGTGGTCAAAGAAAAGACCGGTCTTCAAAAAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1950

Amino Acids

225.76

Weight (kDa)

9.06

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 167 2.3e-18 Vta1 like
FKS1_dom1 PF14288 318 - 430 3.7e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 812 - 1000 3e-64 Callose synthase 1 domain
Glucan_synthase PF02364 1007 - 1097 2.9e-26 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1101 - 1717 9.7e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3801, 5175
AasI GACNNNNNNGTC 1 cut(s) 3745
AatII GACGTC 1 cut(s) 3009
Acc36I ACCTGC 2 cut(s) 659, 4325
Acc65I GGTACC 1 cut(s) 2329
AccB1I GGYRCC 1 cut(s) 2329
AccB7I CCANNNNNTGG 1 cut(s) 1118
AccBSI CCGCTC 2 cut(s) 635, 3606
AccI GTMKAC 2 cut(s) 2097, 5791
AccII CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
AccIII TCCGGA 2 cut(s) 1502, 4515
AcoI YGGCCR 4 cut(s) 1071, 1286, 2555, 2836
AcsI RAATTY 4 cut(s) 3187, 3819, 4928, 5452
AcuI CTGAAG 4 cut(s) 695, 1347, 3503, 4540
AcvI CACGTG 1 cut(s) 332
AcyI GRCGYC 2 cut(s) 3006, 3169
AdeI CACNNNGTG 1 cut(s) 4969
AfaI GTAC 8 cut(s) 942, 1718, 1985, 2331, 4227, 4561, 4617, 5338
AfeI AGCGCT 2 cut(s) 1966, 3834
AflIII ACRYGT 2 cut(s) 139, 2916
AgeI ACCGGT 1 cut(s) 5829
AhdI GACNNNNNGTC 1 cut(s) 669
AjnI CCWGG 2 cut(s) 2305, 5726
AjuI GAANNNNNNNTTGG 2 cut(s) 3856, 3888
AleI CACNNNNGTG 3 cut(s) 3659, 5501, 5614
Alw21I GWGCWC 3 cut(s) 415, 762, 2821
Alw26I GTCTC 5 cut(s) 77, 1929, 2931, 2966, 3721
Ama87I CYCGRG 3 cut(s) 3575, 4835, 5670
Aor13HI TCCGGA 2 cut(s) 1502, 4515
Aor51HI AGCGCT 2 cut(s) 1966, 3834
ApoI RAATTY 4 cut(s) 3187, 3819, 4928, 5452
ArsI GACNNNNNNTTYG 2 cut(s) 4459, 4491
AseI ATTAAT 1 cut(s) 980
AsiGI ACCGGT 1 cut(s) 5829
Asp700I GAANNNNTTC 6 cut(s) 102, 696, 863, 1332, 3347, 3882
Asp718I GGTACC 1 cut(s) 2329
AspS9I GGNCC 5 cut(s) 2060, 2310, 3247, 3926, 4411
AsuC2I CCSGG 1 cut(s) 3924
AsuNHI GCTAGC 2 cut(s) 4207, 5665
AvaI CYCGRG 3 cut(s) 3575, 4835, 5670
AvaII GGWCC 3 cut(s) 2060, 3247, 3926
BaeI ACNNNNGTAYC 6 cut(s) 1397, 1430, 4543, 4576, 5309, 5342
BalI TGGCCA 3 cut(s) 1288, 2557, 2838
BamHI GGATCC 1 cut(s) 1499
BanI GGYRCC 1 cut(s) 2329
BarI GAAGNNNNNNTAC 2 cut(s) 3157, 3189
BauI CACGAG 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 332
BbsI GAAGAC 8 cut(s) 542, 699, 1528, 1549, 4137, 4329, 4909, 5826
Bbv12I GWGCWC 3 cut(s) 415, 762, 2821
BbvCI CCTCAGC 1 cut(s) 1610
BccI CCATC 7 cut(s) 1375, 2413, 3154, 3308, 4735, 5583, 5683
BceAI ACGGC 2 cut(s) 428, 2235
BcgI CGANNNNNNTGC 2 cut(s) 3692, 3726
BciT130I CCWGG 2 cut(s) 2307, 5728
BciVI GTATCC 1 cut(s) 3753
BclI TGATCA 2 cut(s) 2395, 5052
BcnI CCSGG 1 cut(s) 3924
BcoDI GTCTC 5 cut(s) 77, 1929, 2931, 2966, 3721
BfmI CTRYAG 6 cut(s) 627, 1142, 1680, 2098, 2208, 4942
BfoI RGCGCY 4 cut(s) 1518, 1876, 1968, 3836
BfuAI ACCTGC 2 cut(s) 659, 4325
BfuI GTATCC 1 cut(s) 3753
BglII AGATCT 5 cut(s) 3345, 3695, 4422, 5010, 5280
BmcAI AGTACT 1 cut(s) 4617
Bme1390I CCNGG 3 cut(s) 2307, 3924, 5728
Bme18I GGWCC 3 cut(s) 2060, 3247, 3926
BmeRI GACNNNNNGTC 1 cut(s) 669
BmeT110I CYCGRG 3 cut(s) 3575, 4835, 5670
BmgT120I GGNCC 5 cut(s) 2060, 2310, 3247, 3926, 4411
BmiI GGNNCC 6 cut(s) 18, 1501, 2311, 2331, 3927, 4766
BmrFI CCNGG 3 cut(s) 2307, 3924, 5728
BmrI ACTGGG 1 cut(s) 3053
BmsI GCATC 7 cut(s) 316, 1291, 1896, 3458, 3605, 4509, 4785
BmtI GCTAGC 2 cut(s) 4211, 5669
BmuI ACTGGG 1 cut(s) 3053
BpiI GAAGAC 8 cut(s) 542, 699, 1528, 1549, 4137, 4329, 4909, 5826
BplI GAGNNNNNCTC 2 cut(s) 1394, 1426
Bpu10I CCTNAGC 2 cut(s) 1279, 1610
BpuEI CTTGAG 4 cut(s) 1361, 4697, 4775, 5297
BpuMI CCSGG 1 cut(s) 3924
Bsa29I ATCGAT 1 cut(s) 3768
BsaAI YACGTR 3 cut(s) 140, 332, 550
BsaBI GATNNNNATC 2 cut(s) 2541, 5543
BsaHI GRCGYC 2 cut(s) 3006, 3169
BsaI GGTCTC 1 cut(s) 1929
BsaJI CCNNGG 4 cut(s) 659, 2306, 3985, 5179
BsaWI WCCGGW 5 cut(s) 1502, 1736, 2550, 4515, 5829
BsaXI ACNNNNNCTCC 4 cut(s) 2744, 2774, 5219, 5249
Bse118I RCCGGY 2 cut(s) 1073, 5829
Bse3DI GCAATG 3 cut(s) 1482, 2620, 5604
Bse8I GATNNNNATC 2 cut(s) 2541, 5543
BseAI TCCGGA 2 cut(s) 1502, 4515
BseBI CCWGG 2 cut(s) 2307, 5728
BseCI ATCGAT 1 cut(s) 3768
BseDI CCNNGG 4 cut(s) 659, 2306, 3985, 5179
BseJI GATNNNNATC 2 cut(s) 2541, 5543
BseMI GCAATG 3 cut(s) 1482, 2620, 5604
BseRI GAGGAG 5 cut(s) 1215, 2894, 3446, 3827, 5196
BsgI GTGCAG 3 cut(s) 2691, 3198, 4727
Bsh1236I CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
BshNI GGYRCC 1 cut(s) 2329
BshTI ACCGGT 1 cut(s) 5829
BshVI ATCGAT 1 cut(s) 3768
BsiHKAI GWGCWC 3 cut(s) 415, 762, 2821
BsiHKCI CYCGRG 3 cut(s) 3575, 4835, 5670
BsiSI CCGG 8 cut(s) 1074, 1503, 1737, 2551, 3923, 3953, 4516, 5830
BslFI GGGAC 9 cut(s) 981, 1071, 1785, 2942, 3820, 3939, 4200, 4852, 5180
BsmAI GTCTC 5 cut(s) 77, 1929, 2931, 2966, 3721
BsmFI GGGAC 9 cut(s) 981, 1071, 1785, 2942, 3820, 3939, 4200, 4852, 5180
BsmI GAATGC 6 cut(s) 394, 1025, 1216, 2024, 5601, 5766
Bso31I GGTCTC 1 cut(s) 1929
BsoBI CYCGRG 3 cut(s) 3575, 4835, 5670
Bsp1286I GDGCHC 3 cut(s) 415, 762, 2821
Bsp13I TCCGGA 2 cut(s) 1502, 4515
Bsp19I CCATGG 1 cut(s) 659
Bsp68I TCGCGA 1 cut(s) 4479
BspDI ATCGAT 1 cut(s) 3768
BspEI TCCGGA 2 cut(s) 1502, 4515
BspFNI CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
BspHI TCATGA 3 cut(s) 1662, 3009, 4375
BspLI GGNNCC 6 cut(s) 18, 1501, 2311, 2331, 3927, 4766
BspMAI CTGCAG 2 cut(s) 1146, 1684
BspMI ACCTGC 2 cut(s) 659, 4325
BspOI GCTAGC 2 cut(s) 4211, 5669
BspQI GCTCTTC 1 cut(s) 2826
BspT107I GGYRCC 1 cut(s) 2329
BspTNI GGTCTC 1 cut(s) 1929
BsrBI CCGCTC 2 cut(s) 635, 3606
BsrDI GCAATG 3 cut(s) 1482, 2620, 5604
BsrFI RCCGGY 2 cut(s) 1073, 5829
BssAI RCCGGY 2 cut(s) 1073, 5829
BssECI CCNNGG 4 cut(s) 659, 2306, 3985, 5179
BssNI GRCGYC 2 cut(s) 3006, 3169
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 659
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 2307, 5728
Bst6I CTCTTC 7 cut(s) 115, 525, 2469, 2826, 3024, 3423, 5201
BstACI GRCGYC 2 cut(s) 3006, 3169
BstAPI GCANNNNNTGC 2 cut(s) 4204, 4688
BstBAI YACGTR 3 cut(s) 140, 332, 550
BstDSI CCRYGG 2 cut(s) 659, 3985
BstEII GGTNACC 1 cut(s) 2160
BstFNI CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
BstH2I RGCGCY 4 cut(s) 1518, 1876, 1968, 3836
BstMAI GTCTC 5 cut(s) 77, 1929, 2931, 2966, 3721
BstNI CCWGG 2 cut(s) 2307, 5728
BstNSI RCATGY 6 cut(s) 1106, 1837, 2920, 3208, 3235, 5569
BstPI GGTNACC 1 cut(s) 2160
BstSCI CCNGG 3 cut(s) 2305, 3922, 5726
BstSFI CTRYAG 6 cut(s) 627, 1142, 1680, 2098, 2208, 4942
BstUI CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
BstV2I GAAGAC 8 cut(s) 542, 699, 1528, 1549, 4137, 4329, 4909, 5826
BstXI CCANNNNNNTGG 3 cut(s) 3360, 3865, 5589
Bsu15I ATCGAT 1 cut(s) 3768
BsuI GTATCC 1 cut(s) 3753
BsuTUI ATCGAT 1 cut(s) 3768
BtgI CCRYGG 2 cut(s) 659, 3985
BtgZI GCGATG 2 cut(s) 2134, 2361
BtsI GCAGTG 1 cut(s) 3632
BtsIMutI CAGTG 5 cut(s) 1372, 3592, 3632, 4131, 5055
BtuMI TCGCGA 1 cut(s) 4479
BveI ACCTGC 2 cut(s) 659, 4325
CciI TCATGA 3 cut(s) 1662, 3009, 4375
Cfr10I RCCGGY 2 cut(s) 1073, 5829
Cfr13I GGNCC 5 cut(s) 2060, 2310, 3247, 3926, 4411
ClaI ATCGAT 1 cut(s) 3768
CseI GACGC 4 cut(s) 1543, 3158, 5367, 5671
Csp6I GTAC 8 cut(s) 941, 1717, 1984, 2330, 4226, 4560, 4616, 5337
CspAI ACCGGT 1 cut(s) 5829
CviQI GTAC 8 cut(s) 941, 1717, 1984, 2330, 4226, 4560, 4616, 5337
DraI TTTAAA 1 cut(s) 2235
DraIII CACNNNGTG 1 cut(s) 4969
DrdI GACNNNNNNGTC 1 cut(s) 3745
DriI GACNNNNNGTC 1 cut(s) 669
DseDI GACNNNNNNGTC 1 cut(s) 3745
EaeI YGGCCR 4 cut(s) 1071, 1286, 2555, 2836
Eam1104I CTCTTC 7 cut(s) 115, 525, 2469, 2826, 3024, 3423, 5201
Eam1105I GACNNNNNGTC 1 cut(s) 669
EarI CTCTTC 7 cut(s) 115, 525, 2469, 2826, 3024, 3423, 5201
EciI GGCGGA 3 cut(s) 15, 58, 2237
Eco130I CCWWGG 1 cut(s) 659
Eco147I AGGCCT 2 cut(s) 428, 3524
Eco31I GGTCTC 1 cut(s) 1929
Eco47I GGWCC 3 cut(s) 2060, 3247, 3926
Eco47III AGCGCT 2 cut(s) 1966, 3834
Eco57I CTGAAG 4 cut(s) 695, 1347, 3503, 4540
Eco72I CACGTG 1 cut(s) 332
Eco88I CYCGRG 3 cut(s) 3575, 4835, 5670
Eco91I GGTNACC 1 cut(s) 2160
EcoO109I RGGNCCY 1 cut(s) 2310
EcoO65I GGTNACC 1 cut(s) 2160
EcoRII CCWGG 2 cut(s) 2305, 5726
EcoT14I CCWWGG 1 cut(s) 659
EcoT22I ATGCAT 2 cut(s) 1959, 4777
ErhI CCWWGG 1 cut(s) 659
FaqI GGGAC 9 cut(s) 981, 1071, 1785, 2942, 3820, 3939, 4200, 4852, 5180
FauI CCCGC 1 cut(s) 1287
FauNDI CATATG 1 cut(s) 1044
FbaI TGATCA 2 cut(s) 2395, 5052
FblI GTMKAC 2 cut(s) 2097, 5791
HaeII RGCGCY 4 cut(s) 1518, 1876, 1968, 3836
HapII CCGG 8 cut(s) 1074, 1503, 1737, 2551, 3923, 3953, 4516, 5830
HgaI GACGC 4 cut(s) 1543, 3158, 5367, 5671
Hin1I GRCGYC 2 cut(s) 3006, 3169
HincII GTYRAC 5 cut(s) 2653, 3047, 3582, 3674, 4569
HindII GTYRAC 5 cut(s) 2653, 3047, 3582, 3674, 4569
HindIII AAGCTT 8 cut(s) 378, 1134, 1580, 2962, 3078, 3917, 4038, 4296
HpaI GTTAAC 2 cut(s) 3582, 4569
HpaII CCGG 8 cut(s) 1074, 1503, 1737, 2551, 3923, 3953, 4516, 5830
Hpy99I CGWCG 1 cut(s) 3742
HpyCH4IV ACGT 8 cut(s) 139, 331, 549, 1659, 3006, 3709, 3740, 4826
HpySE526I ACGT 8 cut(s) 139, 331, 549, 1659, 3006, 3709, 3740, 4826
Hsp92I GRCGYC 2 cut(s) 3006, 3169
Kpn2I TCCGGA 2 cut(s) 1502, 4515
KpnI GGTACC 1 cut(s) 2333
Ksp22I TGATCA 2 cut(s) 2395, 5052
KspAI GTTAAC 2 cut(s) 3582, 4569
LguI GCTCTTC 1 cut(s) 2826
LmnI GCTCC 9 cut(s) 133, 1099, 1449, 2240, 2406, 3433, 3853, 5101, 5375
LweI GCATC 7 cut(s) 316, 1291, 1896, 3458, 3605, 4509, 4785
MaeII ACGT 8 cut(s) 139, 331, 549, 1659, 3006, 3709, 3740, 4826
MbiI CCGCTC 2 cut(s) 635, 3606
MfeI CAATTG 1 cut(s) 4542
MhlI GDGCHC 3 cut(s) 415, 762, 2821
MlsI TGGCCA 3 cut(s) 1288, 2557, 2838
MluNI TGGCCA 3 cut(s) 1288, 2557, 2838
MlyI GAGTC 6 cut(s) 49, 1150, 2151, 3137, 4363, 4483
Mox20I TGGCCA 3 cut(s) 1288, 2557, 2838
Mph1103I ATGCAT 2 cut(s) 1959, 4777
MroI TCCGGA 2 cut(s) 1502, 4515
MroXI GAANNNNTTC 6 cut(s) 102, 696, 863, 1332, 3347, 3882
MscI TGGCCA 3 cut(s) 1288, 2557, 2838
MslI CAYNNNNRTG 7 cut(s) 851, 3203, 3659, 5078, 5501, 5564, 5614
Msp20I TGGCCA 3 cut(s) 1288, 2557, 2838
MspA1I CMGCKG 4 cut(s) 1325, 1614, 1722, 2103
MspI CCGG 8 cut(s) 1074, 1503, 1737, 2551, 3923, 3953, 4516, 5830
MspR9I CCNGG 3 cut(s) 2307, 3924, 5728
MunI CAATTG 1 cut(s) 4542
Mva1269I GAATGC 6 cut(s) 394, 1025, 1216, 2024, 5601, 5766
MvaI CCWGG 2 cut(s) 2307, 5728
MvnI CGCG 5 cut(s) 266, 2153, 2431, 4479, 4925
NciI CCSGG 1 cut(s) 3924
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 1 cut(s) 1044
NheI GCTAGC 2 cut(s) 4207, 5665
NlaIV GGNNCC 6 cut(s) 18, 1501, 2311, 2331, 3927, 4766
NruI TCGCGA 1 cut(s) 4479
NsiI ATGCAT 2 cut(s) 1959, 4777
NspI RCATGY 6 cut(s) 1106, 1837, 2920, 3208, 3235, 5569
OliI CACNNNNGTG 3 cut(s) 3659, 5501, 5614
PaeI GCATGC 1 cut(s) 5569
PaeR7I CTCGAG 2 cut(s) 3575, 5670
PagI TCATGA 3 cut(s) 1662, 3009, 4375
PceI AGGCCT 2 cut(s) 428, 3524
PciI ACATGT 1 cut(s) 2916
PciSI GCTCTTC 1 cut(s) 2826
PcsI WCGNNNNNNNCGW 3 cut(s) 3743, 3765, 4657
PctI GAATGC 6 cut(s) 394, 1025, 1216, 2024, 5601, 5766
PdmI GAANNNNTTC 6 cut(s) 102, 696, 863, 1332, 3347, 3882
PflMI CCANNNNNTGG 1 cut(s) 1118
PfoI TCCNGGA 1 cut(s) 3922
PinAI ACCGGT 1 cut(s) 5829
PleI GAGTC 6 cut(s) 49, 1150, 2150, 3136, 4363, 4482
PmaCI CACGTG 1 cut(s) 332
PmlI CACGTG 1 cut(s) 332
PpsI GAGTC 6 cut(s) 49, 1150, 2150, 3136, 4363, 4482
Ppu21I YACGTR 3 cut(s) 140, 332, 550
PscI ACATGT 1 cut(s) 2916
PshBI ATTAAT 1 cut(s) 980
PsiI TTATAA 2 cut(s) 3801, 5175
Psp6I CCWGG 2 cut(s) 2305, 5726
PspCI CACGTG 1 cut(s) 332
PspEI GGTNACC 1 cut(s) 2160
PspGI CCWGG 2 cut(s) 2305, 5726
PspN4I GGNNCC 6 cut(s) 18, 1501, 2311, 2331, 3927, 4766
PspPI GGNCC 5 cut(s) 2060, 2310, 3247, 3926, 4411
PspXI VCTCGAGB 1 cut(s) 5670
PsrI GAACNNNNNNTAC 2 cut(s) 2825, 2857
PstI CTGCAG 2 cut(s) 1146, 1684
PvuII CAGCTG 4 cut(s) 1325, 1614, 1722, 2103
RruI TCGCGA 1 cut(s) 4479
RsaI GTAC 8 cut(s) 942, 1718, 1985, 2331, 4227, 4561, 4617, 5338
RsaNI GTAC 8 cut(s) 941, 1717, 1984, 2330, 4226, 4560, 4616, 5337
RseI CAYNNNNRTG 7 cut(s) 851, 3203, 3659, 5078, 5501, 5564, 5614
SapI GCTCTTC 1 cut(s) 2826
Sau96I GGNCC 5 cut(s) 2060, 2310, 3247, 3926, 4411
ScaI AGTACT 1 cut(s) 4617
SchI GAGTC 6 cut(s) 49, 1150, 2151, 3137, 4363, 4483
ScrFI CCNGG 3 cut(s) 2307, 3924, 5728
SduI GDGCHC 3 cut(s) 415, 762, 2821
SfaNI GCATC 7 cut(s) 316, 1291, 1896, 3458, 3605, 4509, 4785
SfcI CTRYAG 6 cut(s) 627, 1142, 1680, 2098, 2208, 4942
Sfr274I CTCGAG 2 cut(s) 3575, 5670
SinI GGWCC 3 cut(s) 2060, 3247, 3926
SlaI CTCGAG 2 cut(s) 3575, 5670
SmiMI CAYNNNNRTG 7 cut(s) 851, 3203, 3659, 5078, 5501, 5564, 5614
SmlI CTYRAG 6 cut(s) 1340, 3575, 4676, 4754, 5276, 5670
SmoI CTYRAG 6 cut(s) 1340, 3575, 4676, 4754, 5276, 5670
SphI GCATGC 1 cut(s) 5569
SseBI AGGCCT 2 cut(s) 428, 3524
SspI AATATT 3 cut(s) 1253, 2861, 3038
StuI AGGCCT 2 cut(s) 428, 3524
StyD4I CCNGG 3 cut(s) 2305, 3922, 5726
StyI CCWWGG 1 cut(s) 659
TaiI ACGT 8 cut(s) 142, 334, 552, 1662, 3009, 3712, 3743, 4829
TaqII GACCGA 1 cut(s) 3235
TatI WGTACW 2 cut(s) 1983, 4615
TauI GCSGC 2 cut(s) 635, 1679
TscAI CASTG 5 cut(s) 1372, 3592, 3632, 4138, 5062
TspGWI ACGGA 8 cut(s) 2083, 3089, 3735, 4254, 4649, 4804, 4872, 5103
TspRI CASTG 5 cut(s) 1372, 3592, 3632, 4138, 5062
Van91I CCANNNNNTGG 1 cut(s) 1118
VpaK11BI GGWCC 3 cut(s) 2060, 3247, 3926
VspI ATTAAT 1 cut(s) 980
XapI RAATTY 4 cut(s) 3187, 3819, 4928, 5452
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 6 cut(s) 1106, 1837, 2920, 3208, 3235, 5569
XhoI CTCGAG 2 cut(s) 3575, 5670
XmiI GTMKAC 2 cut(s) 2097, 5791
XmnI GAANNNNTTC 6 cut(s) 102, 696, 863, 1332, 3347, 3882
ZraI GACGTC 1 cut(s) 3007
ZrmI AGTACT 1 cut(s) 4617
Zsp2I ATGCAT 2 cut(s) 1959, 4777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.