Rroxscaffold_4G00332260

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002059
Physical Location & Seq
Reverse (-)
66318033 .. 66337631
19599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332260.1

Sequence Viewer

Length: 5853 bp
ATGGCATATAGAAGGGGCTTCGATCAGCAGCAACCGCAGAGGCGAATTCTGCGGACTCAGACTGCCGGCGCCGGCAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGTTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCACAGTACTGCTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTGTTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTCCCTCTTGATCCTGATAGTCGAAATCAGGCAATCATGAGATATCCCGAGATTCTTGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGCCGAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTATTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACAGTAGTGATGAAGAAACTTTTCAAAAATTACAAAAAATGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTACAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCCGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACCTGAATGAATACTTTTGGTCAGTAGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACCATACACCGGGGATCGATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTTCAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCGCAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCCGTGACTTATGCATATAGTTGGAAAAATCCCTCTGGGTTTACACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTTTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGCATCTTGAAAGATCAAACTATAGGATTATGATGCTGATGATGTGGTGGTCTCAGACTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCAATCTTCTCCACAATCTATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTGGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAGTGCTTCCTTGATTCCTGAGGAGAAAAAAGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCATCCAATATAGAGAATGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCACTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGAACCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCTGTTAGAGAGTGCTATCTTTCGTTTCGAAGTATTATCAATGTTTTAGTCCAAGGAGAACGCGAAAAGATGGTTATAAATGAAATTTTTTACATAGTTGATGGTCATATAGAAAAAGGAACACTGACGAAGGAAGTTAAATTGAGTGCTCTCCCCAGCCTCCACGAACAATTTATTAAGCTAATTGATTATCTGTTGAAAAACAAAAGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGAAGATGAAATTACCACCTTGGTAGATTCAAACCATGGTGGATCATATGGAAACGATGGGGGAATGAGACCTCTTAATCAACAAGATACATACTTCAGTGAACTGAATTTCCCAGTGACGCAGCAATCAGAGGCTTGGAAAGAAAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGACATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCGGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAACGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACTAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAACTCAGAGCAAACGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTATAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGAAAGGCCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCTGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCTCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTTGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAACGGTGAGCAGACACTGAGTCGTGACATCTATAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTCGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTAATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTCGGTACAAAGACTCACTATTATGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACTGTACAGATATGGTTCATGGTTGGAACATGGCTTTTCGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATTGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTACTCTGGGATGCGCGGTATCATTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAAAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATCGTTGTTTTGTTGCTGATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTTTTGTTTCGACTTGTTAAGGGATGTATGTTTGTTACACTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATTCTTTTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCGGTCGGTTCAAACACTTGCTCGTGCTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCTTGGTTCCCTTTTGTTTCTGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGTCCCCGCGCTAGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1950

Amino Acids

225.16

Weight (kDa)

9.28

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 170 2.4e-16 Vta1 like
FKS1_dom1 PF14288 319 - 431 8.8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 854 - 1043 2.6e-62 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1141 5.2e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1145 - 1845 4.5e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2756
AasI GACNNNNNNGTC 1 cut(s) 1538
Acc16I TGCGCA 1 cut(s) 1593
Acc36I ACCTGC 2 cut(s) 3946, 4331
AccB1I GGYRCC 2 cut(s) 68, 1425
AccB7I CCANNNNNTGG 1 cut(s) 1121
AccBSI CCGCTC 1 cut(s) 4629
AccI GTMKAC 3 cut(s) 1988, 3831, 5313
AccII CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
AclWI GGATC 9 cut(s) 221, 572, 1386, 2398, 2490, 2578, 3004, 3097, 3881
AcoI YGGCCR 3 cut(s) 241, 665, 4246
AcuI CTGAAG 4 cut(s) 698, 3034, 3426, 3512
AcvI CACGTG 1 cut(s) 3995
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 4787
AfeI AGCGCT 1 cut(s) 3519
AflII CTTAAG 1 cut(s) 4321
AflIII ACRYGT 2 cut(s) 2925, 3992
AhdI GACNNNNNGTC 1 cut(s) 4479
AhlI ACTAGT 3 cut(s) 2465, 3794, 4184
AjuI GAANNNNNNNTTGG 4 cut(s) 995, 1027, 1777, 1809
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 4 cut(s) 3048, 3080, 4754, 4786
Alw21I GWGCWC 6 cut(s) 418, 765, 1107, 2091, 2830, 5532
Alw26I GTCTC 4 cut(s) 1932, 3016, 3334, 4175
Alw44I GTGCAC 2 cut(s) 414, 2087
AlwI GGATC 9 cut(s) 221, 572, 1386, 2398, 2490, 2578, 3004, 3097, 3881
AlwNI CAGNNNCTG 2 cut(s) 488, 4477
Ama87I CYCGRG 2 cut(s) 614, 2124
Aor51HI AGCGCT 1 cut(s) 3519
ApaLI GTGCAC 2 cut(s) 414, 2087
AseI ATTAAT 1 cut(s) 4799
Asp700I GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
AspA2I CCTAGG 1 cut(s) 5819
AspLEI GCGC 8 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304, 5837
AspS9I GGNCC 9 cut(s) 1781, 2899, 2947, 3904, 3935, 4690, 4816, 5816, 5828
AsuC2I CCSGG 3 cut(s) 1078, 1375, 5711
AsuHPI GGTGA 9 cut(s) 1112, 1139, 1547, 3293, 3722, 4478, 4534, 5327, 5401
AsuII TTCGAA 3 cut(s) 2018, 2707, 4666
AsuNHI GCTAGC 4 cut(s) 1072, 2576, 4217, 5837
AvaI CYCGRG 2 cut(s) 614, 2124
AvaII GGWCC 6 cut(s) 1781, 2899, 2947, 3904, 3935, 5828
AvrII CCTAGG 1 cut(s) 5819
AxyI CCTNAGG 1 cut(s) 2337
BaeGI GKGCMC 2 cut(s) 418, 2091
BaeI ACNNNNGTAYC 2 cut(s) 5344, 5377
BalI TGGCCA 1 cut(s) 4248
BanI GGYRCC 2 cut(s) 68, 1425
BanII GRGCYC 1 cut(s) 2167
BarI GAAGNNNNNNTAC 4 cut(s) 2198, 2230, 3166, 3198
BauI CACGAG 4 cut(s) 333, 3717, 5243, 5670
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 4 cut(s) 702, 3285, 3435, 3807
Bbv12I GWGCWC 6 cut(s) 418, 765, 1107, 2091, 2830, 5532
BbvCI CCTCAGC 1 cut(s) 501
BceAI ACGGC 3 cut(s) 73, 228, 812
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciVI GTATCC 2 cut(s) 2185, 5372
BclI TGATCA 4 cut(s) 814, 1485, 4314, 5391
BcnI CCSGG 3 cut(s) 1078, 1375, 5711
BcoDI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BcuI ACTAGT 3 cut(s) 2465, 3794, 4184
BfmI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
BfoI RGCGCY 2 cut(s) 72, 3521
BfrI CTTAAG 1 cut(s) 4321
BfuAI ACCTGC 2 cut(s) 3946, 4331
BfuI GTATCC 2 cut(s) 2185, 5372
BglII AGATCT 2 cut(s) 3559, 4428
BlnI CCTAGG 1 cut(s) 5819
BmcAI AGTACT 3 cut(s) 394, 896, 4651
Bme18I GGWCC 6 cut(s) 1781, 2899, 2947, 3904, 3935, 5828
BmeRI GACNNNNNGTC 1 cut(s) 4479
BmeT110I CYCGRG 2 cut(s) 614, 2124
BmgT120I GGNCC 9 cut(s) 1781, 2899, 2947, 3904, 3935, 4690, 4816, 5816, 5828
BmrI ACTGGG 1 cut(s) 3062
BmsI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
BmtI GCTAGC 4 cut(s) 1076, 2580, 4221, 5841
BmuI ACTGGG 1 cut(s) 3062
BpiI GAAGAC 4 cut(s) 702, 3285, 3435, 3807
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 1400, 1432
BpmI CTGGAG 1 cut(s) 2297
Bpu10I CCTNAGC 4 cut(s) 501, 519, 525, 4121
Bpu14I TTCGAA 3 cut(s) 2018, 2707, 4666
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 3 cut(s) 1078, 1375, 5711
Bsa29I ATCGAT 1 cut(s) 1381
BsaAI YACGTR 3 cut(s) 3995, 4361, 5804
BsaBI GATNNNNATC 3 cut(s) 2299, 3558, 3915
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 2 cut(s) 1932, 3016
BsaJI CCNNGG 9 cut(s) 244, 662, 1374, 1692, 2731, 2973, 2989, 5709, 5819
BsaWI WCCGGW 1 cut(s) 3510
BsaXI ACNNNNNCTCC 6 cut(s) 1072, 1102, 3122, 3152, 4415, 4445
Bse118I RCCGGY 4 cut(s) 65, 71, 1875, 2253
Bse1I ACTGG 9 cut(s) 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317, 5590
Bse21I CCTNAGG 1 cut(s) 2337
Bse3DI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
Bse8I GATNNNNATC 3 cut(s) 2299, 3558, 3915
BseCI ATCGAT 1 cut(s) 1381
BseDI CCNNGG 9 cut(s) 244, 662, 1374, 1692, 2731, 2973, 2989, 5709, 5819
BseJI GATNNNNATC 3 cut(s) 2299, 3558, 3915
BseMI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
BseNI ACTGG 9 cut(s) 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317, 5590
BseRI GAGGAG 4 cut(s) 518, 1148, 2354, 3849
BseSI GKGCMC 2 cut(s) 418, 2091
BseYI CCCAGC 1 cut(s) 2834
BsgI GTGCAG 5 cut(s) 621, 3207, 4764, 4908, 5469
Bsh1236I CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
Bsh1285I CGRYCG 3 cut(s) 3597, 4609, 5654
BshNI GGYRCC 2 cut(s) 68, 1425
BshVI ATCGAT 1 cut(s) 1381
BsiEI CGRYCG 3 cut(s) 3597, 4609, 5654
BsiHKAI GWGCWC 6 cut(s) 418, 765, 1107, 2091, 2830, 5532
BsiHKCI CYCGRG 2 cut(s) 614, 2124
BsiSI CCGG 8 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511, 5711
BslFI GGGAC 4 cut(s) 2382, 2504, 2960, 5814
BsmAI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BsmFI GGGAC 4 cut(s) 2382, 2504, 2960, 5814
Bso31I GGTCTC 2 cut(s) 1932, 3016
BsoBI CYCGRG 2 cut(s) 614, 2124
Bsp119I TTCGAA 3 cut(s) 2018, 2707, 4666
Bsp1286I GDGCHC 7 cut(s) 418, 765, 1107, 2091, 2167, 2830, 5532
Bsp1407I TGTACA 1 cut(s) 5065
Bsp19I CCATGG 1 cut(s) 2989
BspDI ATCGAT 1 cut(s) 1381
BspFNI CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
BspHI TCATGA 3 cut(s) 603, 2079, 4381
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 2 cut(s) 3946, 4331
BspOI GCTAGC 4 cut(s) 1076, 2580, 4221, 5841
BspPI GGATC 9 cut(s) 221, 572, 1386, 2398, 2490, 2578, 3004, 3097, 3881
BspT104I TTCGAA 3 cut(s) 2018, 2707, 4666
BspT107I GGYRCC 2 cut(s) 68, 1425
BspTI CTTAAG 1 cut(s) 4321
BspTNI GGTCTC 2 cut(s) 1932, 3016
BsrBI CCGCTC 1 cut(s) 4629
BsrDI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
BsrFI RCCGGY 4 cut(s) 65, 71, 1875, 2253
BsrGI TGTACA 1 cut(s) 5065
BsrI ACTGG 9 cut(s) 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317, 5590
BssAI RCCGGY 4 cut(s) 65, 71, 1875, 2253
BssECI CCNNGG 9 cut(s) 244, 662, 1374, 1692, 2731, 2973, 2989, 5709, 5819
BssNAI GTATAC 1 cut(s) 1989
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 4 cut(s) 333, 3717, 5243, 5670
BssT1I CCWWGG 4 cut(s) 2731, 2973, 2989, 5819
Bst1107I GTATAC 1 cut(s) 1989
Bst2BI CACGAG 4 cut(s) 333, 3717, 5243, 5670
Bst6I CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
BstACI GRCGYC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 4321
BstAPI GCANNNNNTGC 2 cut(s) 179, 2438
BstAUI TGTACA 1 cut(s) 5065
BstBAI YACGTR 3 cut(s) 3995, 4361, 5804
BstBI TTCGAA 3 cut(s) 2018, 2707, 4666
BstDSI CCRYGG 2 cut(s) 244, 2989
BstENI CCTNNNNNAGG 1 cut(s) 1651
BstFNI CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
BstH2I RGCGCY 2 cut(s) 72, 3521
BstHHI GCGC 8 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304, 5837
BstMAI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BstMCI CGRYCG 3 cut(s) 3597, 4609, 5654
BstNSI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
BstSFI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
BstSLI GKGCMC 2 cut(s) 418, 2091
BstUI CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
BstV2I GAAGAC 4 cut(s) 702, 3285, 3435, 3807
BstX2I RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
BstXI CCANNNNNNTGG 5 cut(s) 3369, 3689, 3817, 5021, 5589
BstYI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
BstZ17I GTATAC 1 cut(s) 1989
Bsu15I ATCGAT 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 2337
BsuI GTATCC 2 cut(s) 2185, 5372
BsuTUI ATCGAT 1 cut(s) 1381
BtgI CCRYGG 2 cut(s) 244, 2989
BtsI GCAGTG 2 cut(s) 2303, 4150
BveI ACCTGC 2 cut(s) 3946, 4331
CaiI CAGNNNCTG 2 cut(s) 488, 4477
CciI TCATGA 3 cut(s) 603, 2079, 4381
CfoI GCGC 8 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304, 5837
Cfr10I RCCGGY 4 cut(s) 65, 71, 1875, 2253
Cfr13I GGNCC 9 cut(s) 1781, 2899, 2947, 3904, 3935, 4690, 4816, 5816, 5828
ClaI ATCGAT 1 cut(s) 1381
CseI GACGC 1 cut(s) 3082
CsiI ACCWGGT 1 cut(s) 2901
DinI GGCGCC 1 cut(s) 70
DraI TTTAAA 2 cut(s) 262, 1546
DraIII CACNNNGTG 1 cut(s) 4787
DrdI GACNNNNNNGTC 1 cut(s) 1538
DriI GACNNNNNGTC 1 cut(s) 4479
DseDI GACNNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 3 cut(s) 241, 665, 4246
Eam1104I CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
Eam1105I GACNNNNNGTC 1 cut(s) 4479
EarI CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
EciI GGCGGA 1 cut(s) 3437
Eco130I CCWWGG 4 cut(s) 2731, 2973, 2989, 5819
Eco147I AGGCCT 3 cut(s) 1139, 2053, 3533
Eco24I GRGCYC 1 cut(s) 2167
Eco31I GGTCTC 2 cut(s) 1932, 3016
Eco32I GATATC 2 cut(s) 611, 2077
Eco47I GGWCC 6 cut(s) 1781, 2899, 2947, 3904, 3935, 5828
Eco47III AGCGCT 1 cut(s) 3519
Eco57I CTGAAG 4 cut(s) 698, 3034, 3426, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco81I CCTNAGG 1 cut(s) 2337
Eco88I CYCGRG 2 cut(s) 614, 2124
EcoNI CCTNNNNNAGG 1 cut(s) 1651
EcoO109I RGGNCCY 3 cut(s) 2947, 5816, 5828
EcoRI GAATTC 3 cut(s) 45, 5595, 5747
EcoRV GATATC 2 cut(s) 611, 2077
EcoT14I CCWWGG 4 cut(s) 2731, 2973, 2989, 5819
EcoT22I ATGCAT 3 cut(s) 205, 1723, 1962
EcoT38I GRGCYC 1 cut(s) 2167
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 4 cut(s) 2731, 2973, 2989, 5819
FaqI GGGAC 4 cut(s) 2382, 2504, 2960, 5814
FauI CCCGC 1 cut(s) 5840
FauNDI CATATG 4 cut(s) 1047, 3001, 3667, 5538
FbaI TGATCA 4 cut(s) 814, 1485, 4314, 5391
FblI GTMKAC 3 cut(s) 1988, 3831, 5313
FriOI GRGCYC 1 cut(s) 2167
FspI TGCGCA 1 cut(s) 1593
GlaI GCGC 8 cut(s) 70, 1593, 1969, 2675, 3519, 5270, 5303, 5836
GsaI CCCAGC 1 cut(s) 2838
GsuI CTGGAG 1 cut(s) 2297
HaeII RGCGCY 2 cut(s) 72, 3521
HapII CCGG 8 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511, 5711
HgaI GACGC 1 cut(s) 3082
HhaI GCGC 8 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304, 5837
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 8 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302, 5835
HinP1I GCGC 8 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302, 5835
HincII GTYRAC 3 cut(s) 1345, 3652, 4575
HindII GTYRAC 3 cut(s) 1345, 3652, 4575
HindIII AAGCTT 5 cut(s) 381, 1586, 3926, 4302, 5613
HpaI GTTAAC 1 cut(s) 4575
HpaII CCGG 8 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511, 5711
HphI GGTGA 9 cut(s) 1112, 1139, 1547, 3293, 3722, 4478, 4534, 5327, 5401
Hpy99I CGWCG 1 cut(s) 5442
HpyCH4IV ACGT 5 cut(s) 280, 3282, 3994, 4360, 5803
HpySE526I ACGT 5 cut(s) 280, 3282, 3994, 4360, 5803
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 8 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302, 5835
KasI GGCGCC 1 cut(s) 68
KflI GGGWCCC 1 cut(s) 5828
KroI GCCGGC 4 cut(s) 65, 71, 1875, 2253
KroNI GCCGGC 4 cut(s) 67, 73, 1877, 2255
Ksp22I TGATCA 4 cut(s) 814, 1485, 4314, 5391
KspAI GTTAAC 1 cut(s) 4575
LmnI GCTCC 8 cut(s) 136, 760, 1197, 1455, 2170, 3862, 4126, 5107
LweI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
MabI ACCWGGT 1 cut(s) 2901
MaeII ACGT 5 cut(s) 280, 3282, 3994, 4360, 5803
MbiI CCGCTC 1 cut(s) 4629
MfeI CAATTG 3 cut(s) 2169, 3873, 4811
MflI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
MhlI GDGCHC 7 cut(s) 418, 765, 1107, 2091, 2167, 2830, 5532
MlsI TGGCCA 1 cut(s) 4248
MluNI TGGCCA 1 cut(s) 4248
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 7 cut(s) 49, 1927, 2266, 3146, 4489, 4626, 4846
MmeI TCCRAC 8 cut(s) 72, 810, 1709, 1927, 3219, 3887, 4130, 5065
Mox20I TGGCCA 1 cut(s) 4248
Mph1103I ATGCAT 3 cut(s) 205, 1723, 1962
MroNI GCCGGC 4 cut(s) 65, 71, 1875, 2253
MroXI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
MscI TGGCCA 1 cut(s) 4248
MslI CAYNNNNRTG 6 cut(s) 2084, 2446, 4254, 4860, 4884, 5537
Msp20I TGGCCA 1 cut(s) 4248
MspA1I CMGCKG 3 cut(s) 952, 1685, 5429
MspCI CTTAAG 1 cut(s) 4321
MspI CCGG 8 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511, 5711
MunI CAATTG 3 cut(s) 2169, 3873, 4811
MvnI CGCG 5 cut(s) 1970, 2742, 5271, 5758, 5835
NaeI GCCGGC 4 cut(s) 67, 73, 1877, 2255
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 3 cut(s) 1078, 1375, 5711
NcoI CCATGG 1 cut(s) 2989
NdeI CATATG 4 cut(s) 1047, 3001, 3667, 5538
NgoMIV GCCGGC 4 cut(s) 65, 71, 1875, 2253
NheI GCTAGC 4 cut(s) 1072, 2576, 4217, 5837
NmeAIII GCCGAG 1 cut(s) 1915
NmuCI GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
NsbI TGCGCA 1 cut(s) 1593
NsiI ATGCAT 3 cut(s) 205, 1723, 1962
NspI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
NspV TTCGAA 3 cut(s) 2018, 2707, 4666
OliI CACNNNNGTG 1 cut(s) 2446
PaeR7I CTCGAG 1 cut(s) 2124
PagI TCATGA 3 cut(s) 603, 2079, 4381
PceI AGGCCT 3 cut(s) 1139, 2053, 3533
PciI ACATGT 1 cut(s) 2925
PcsI WCGNNNNNNNCGW 1 cut(s) 4663
PdiI GCCGGC 4 cut(s) 67, 73, 1877, 2255
PdmI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
PflMI CCANNNNNTGG 1 cut(s) 1121
PfoI TCCNGGA 2 cut(s) 3537, 3931
Ple19I CGATCG 1 cut(s) 3597
PleI GAGTC 7 cut(s) 49, 1927, 2266, 3145, 4488, 4626, 4846
PluTI GGCGCC 1 cut(s) 72
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 7 cut(s) 49, 1927, 2266, 3145, 4488, 4626, 4846
Ppu21I YACGTR 3 cut(s) 3995, 4361, 5804
PpuMI RGGWCCY 2 cut(s) 2947, 5828
PscI ACATGT 1 cut(s) 2925
PshBI ATTAAT 1 cut(s) 4799
PsiI TTATAA 1 cut(s) 2756
Psp5II RGGWCCY 2 cut(s) 2947, 5828
PspCI CACGTG 1 cut(s) 3995
PspFI CCCAGC 1 cut(s) 2834
PspPI GGNCC 9 cut(s) 1781, 2899, 2947, 3904, 3935, 4690, 4816, 5816, 5828
PspPPI RGGWCCY 2 cut(s) 2947, 5828
PspXI VCTCGAGB 1 cut(s) 2124
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 2 cut(s) 488, 4477
PsuI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
PvuI CGATCG 1 cut(s) 3597
PvuII CAGCTG 2 cut(s) 1685, 5429
RseI CAYNNNNRTG 6 cut(s) 2084, 2446, 4254, 4860, 4884, 5537
Sau96I GGNCC 9 cut(s) 1781, 2899, 2947, 3904, 3935, 4690, 4816, 5816, 5828
ScaI AGTACT 3 cut(s) 394, 896, 4651
SchI GAGTC 7 cut(s) 49, 1927, 2266, 3146, 4489, 4626, 4846
SduI GDGCHC 7 cut(s) 418, 765, 1107, 2091, 2167, 2830, 5532
SexAI ACCWGGT 1 cut(s) 2901
SfaNI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
SfcI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
SfoI GGCGCC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 2124
SfuI TTCGAA 3 cut(s) 2018, 2707, 4666
SinI GGWCC 6 cut(s) 1781, 2899, 2947, 3904, 3935, 5828
SlaI CTCGAG 1 cut(s) 2124
SmiMI CAYNNNNRTG 6 cut(s) 2084, 2446, 4254, 4860, 4884, 5537
SmlI CTYRAG 3 cut(s) 1006, 2124, 4321
SmoI CTYRAG 3 cut(s) 1006, 2124, 4321
SpeI ACTAGT 3 cut(s) 2465, 3794, 4184
SseBI AGGCCT 3 cut(s) 1139, 2053, 3533
SspDI GGCGCC 1 cut(s) 68
SspI AATATT 1 cut(s) 565
StuI AGGCCT 3 cut(s) 1139, 2053, 3533
StyI CCWWGG 4 cut(s) 2731, 2973, 2989, 5819
TaiI ACGT 5 cut(s) 283, 3285, 3997, 4363, 5806
TatI WGTACW 7 cut(s) 392, 894, 943, 2181, 4649, 4893, 5065
TauI GCSGC 2 cut(s) 3625, 3857
TseFI GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
Tsp45I GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
TspGWI ACGGA 3 cut(s) 131, 3143, 4763
Van91I CCANNNNNTGG 1 cut(s) 1121
Vha464I CTTAAG 1 cut(s) 4321
VneI GTGCAC 2 cut(s) 414, 2087
VpaK11BI GGWCC 6 cut(s) 1781, 2899, 2947, 3904, 3935, 5828
VspI ATTAAT 1 cut(s) 4799
XagI CCTNNNNNAGG 1 cut(s) 1651
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3301
XhoI CTCGAG 1 cut(s) 2124
XmaJI CCTAGG 1 cut(s) 5819
XmiI GTMKAC 3 cut(s) 1988, 3831, 5313
XmnI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
ZrmI AGTACT 3 cut(s) 394, 896, 4651
Zsp2I ATGCAT 3 cut(s) 205, 1723, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.