RchiOBHm_Chr1g0313311

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002059
Physical Location & Seq
Forward (+)
430098 .. 431425
1328 bp
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UTR
Exon/CDS
Intron
PRQ54413

Sequence Viewer

Length: 315 bp
ATGGCATATAGAAGGGGCTTCGACCAGCAGCAACCGCAGAGGCGGATTCTGCGGACTCAGACTGCCGGAAGTTTTGGAGAGCCTATGTTGGATAGTGATGTTGTGCCTTCTTCTCTGGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGCTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAACTCTATCAGGAAATTGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

104

Amino Acids

11.91

Weight (kDa)

9.73

Isoelectric Point (pI)

61.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 96 6.3e-10 Vta1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 35, 43, 52, 264
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 1 cut(s) 238
AgsI TTSAA 1 cut(s) 275
AluBI AGCT 2 cut(s) 212, 256
AluI AGCT 2 cut(s) 212, 256
AlwI GGATC 1 cut(s) 218
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 225
BfaI CTAG 4 cut(s) 162, 221, 234, 281
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 40
BshFI GGCC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 66
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 1 cut(s) 240
Bsp143I GATC 1 cut(s) 223
BspACI CCGC 4 cut(s) 35, 43, 52, 264
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 1 cut(s) 223
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 241
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 3 cut(s) 34, 49, 176
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 254
CviJI RGCY 5 cut(s) 18, 82, 212, 240, 256
CviKI_1 RGCY 5 cut(s) 18, 82, 212, 240, 256
DdeI CTNAG 1 cut(s) 57
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 238
EciI GGCGGA 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 202
FaiI YATR 4 cut(s) 7, 9, 86, 198
Fnu4HI GCNGC 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 29
FspBI CTAG 4 cut(s) 162, 221, 234, 281
GluI GCNGC 1 cut(s) 29
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 66
HinfI GANTC 3 cut(s) 46, 55, 158
HpaII CCGG 1 cut(s) 66
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 2 cut(s) 281, 299
HpyAV CCTTC 2 cut(s) 6, 117
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 3 cut(s) 146, 170, 200
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 49, 176
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 277
Kzo9I GATC 1 cut(s) 223
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 5 cut(s) 38, 79, 101, 266, 284
Lsp1109I GCAGC 1 cut(s) 40
MaeI CTAG 4 cut(s) 162, 221, 234, 281
MaeII ACGT 1 cut(s) 277
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MboII GAAGA 2 cut(s) 102, 263
MflI RGATCY 1 cut(s) 223
MluCI AATT 1 cut(s) 303
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 2 cut(s) 33, 279
Mph1103I ATGCAT 1 cut(s) 202
MseI TTAA 1 cut(s) 258
MspI CCGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 172
MwoI GCNNNNNNNGC 3 cut(s) 34, 49, 176
NdeII GATC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 202
PctI GAATGC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 46, 158
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PsuI RGATCY 1 cut(s) 223
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 1 cut(s) 223
SchI GAGTC 1 cut(s) 49
SetI ASST 3 cut(s) 214, 258, 280
Sse9I AATT 1 cut(s) 303
SsiI CCGC 4 cut(s) 35, 43, 52, 264
SspMI CTAG 4 cut(s) 162, 221, 234, 281
TaiI ACGT 1 cut(s) 280
TaqI TCGA 2 cut(s) 21, 156
TasI AATT 1 cut(s) 303
TfiI GAWTC 2 cut(s) 46, 158
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 1 cut(s) 128
XbaI TCTAGA 1 cut(s) 280
XspI CTAG 4 cut(s) 162, 221, 234, 281
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.