RLG00000030802

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002059
Physical Location & Seq
Reverse (-)
67759898 .. 67777891
17994 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030802

Sequence Viewer

Length: 5733 bp
ATGGCATATAGAAGGCGCTTCGATCAGCAGCAACCGCAGAGGCCGATTCTACGGACTCAGACTGCCGGAAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAAGCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGTTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCGAGATTACTACCAAAAATACATTCAAGCTTTGCACACTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTGTTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGATGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTTCCTCTTGATCCTGATAGTCAAAATCAGGCAATCATGAGATATCCCGAGATTCATGCTACTGTTTCTGCACTTCGAAACACCAGAGGTTTACCCTGGCCGAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTGTTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCTAAGCAGGAAGTACAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCTGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACCTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGATAAAGAAACCATACACCGGGGATCAATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTTCAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCACAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCCGTGACTTATGCATATAGTTGGAAAAATCCCTCTGGGTTTGCACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTGTTCATTCTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGCTTCTTGAAAGATCAAACTATAAGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGTGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTTGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATAATGAGAGTGCACATTTCGATCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTTTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTAGATTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCATCTAATAAAGAGAAGGAGGCAGCAAAATTTGCTCAGATGTGGAATGAAATAATCAGTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTAGTCCCATATTGGGCTGACCCAGATTTGAACCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCTGTTAGAGAGTGCTATCTTTCGTTTCGAAGTATTATCAATGTTTTAGTCCATGGAGAACGTGAAAAGATGGTTATAAATGAAATTTTTTCCATAGTTGATCATCATATAGAACAAGGAACGCTGACGAAGGAAGTTAAATTGAGTGCCCTCCCAAGCCTCCACGAACAATTTATTATGCTAATTGATTATCTGTTGAAAAACAAAAGGGAGGACAAGGATCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATTGATGATGAAATTACCACCTTGCTAGATTCAAACCATGGTGGATCATATGGAAACGATGGGGGAATGAGACCCCTTAATCAACAAGATTCATACTTCAGTGAACTGAATTTCCCAGTGACGCCACAAACAGAGGCTTGGAAAGAAAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGACATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCAGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAACGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACTAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAACTCAGAGGAAACGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCTTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTATAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGAAAGGCCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAATACCATGATGGCAGACCTCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTGACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTGGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAATGGTGAGCAGACACTGAGTCGTGACATCTACAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGTGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAGACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTGATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTCGGTACAAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTTAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACCGTACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATCGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTACTCTGGGATGCGCGGTATCATTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAAAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATCGTTGTTTTGTTGCTTATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTTTTGTTTCGACTTGTTAAGGGATGTATGTTTATTACACTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTTTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCAGTCGGTTCAAACACTTGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCTTGGTTCCCTTTTGTTTCCGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGCCCCCGCGCTGGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1911

Amino Acids

220.37

Weight (kDa)

9.09

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 40 - 169 1.9e-14 Vta1 like
FKS1_dom1 PF14288 279 - 391 8.6e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 814 - 1003 1.1e-61 Callose synthase 1 domain
Glucan_synthase PF02364 1011 - 1099 7.3e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1106 - 1803 6.2e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2636
AasI GACNNNNNNGTC 1 cut(s) 1418
Acc36I ACCTGC 1 cut(s) 4211
AccB1I GGYRCC 1 cut(s) 1305
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccI GTMKAC 3 cut(s) 1868, 3711, 5193
AccII CGCG 3 cut(s) 5151, 5638, 5715
AciI CCGC 9 cut(s) 35, 261, 832, 3328, 3503, 3734, 3819, 5151, 5713
AcoI YGGCCR 3 cut(s) 235, 653, 4126
AcsI RAATTY 9 cut(s) 2079, 2273, 2315, 2643, 2941, 3539, 3956, 4814, 5627
AcuI CTGAAG 4 cut(s) 686, 2914, 3306, 3392
AcvI CACGTG 1 cut(s) 3875
AcyI GRCGYC 1 cut(s) 2954
AdeI CACNNNGTG 1 cut(s) 4667
AfaI GTAC 9 cut(s) 825, 3283, 3372, 4531, 4657, 4726, 4775, 4892, 4947
AfeI AGCGCT 1 cut(s) 3399
AflII CTTAAG 1 cut(s) 4201
AflIII ACRYGT 2 cut(s) 2805, 3872
AhdI GACNNNNNGTC 1 cut(s) 4359
AhlI ACTAGT 2 cut(s) 3674, 4064
AjnI CCWGG 8 cut(s) 650, 1571, 3183, 3786, 3811, 3906, 5105, 5244
AjuI GAANNNNNNNTTGG 6 cut(s) 354, 386, 875, 907, 1657, 1689
AloI GAACNNNNNNTCC 4 cut(s) 2928, 2960, 4634, 4666
Alw21I GWGCWC 5 cut(s) 406, 753, 987, 1971, 5412
Alw26I GTCTC 5 cut(s) 1812, 2896, 3214, 4055, 4542
Alw44I GTGCAC 2 cut(s) 402, 1967
AlwNI CAGNNNCTG 2 cut(s) 476, 4357
Ama87I CYCGRG 2 cut(s) 602, 2004
Aor51HI AGCGCT 1 cut(s) 3399
ApaI GGGCCC 1 cut(s) 5711
ApaLI GTGCAC 2 cut(s) 402, 1967
ApoI RAATTY 9 cut(s) 2079, 2273, 2315, 2643, 2941, 3539, 3956, 4814, 5627
ArsI GACNNNNNNTTYG 2 cut(s) 2143, 2175
AseI ATTAAT 1 cut(s) 4679
Asp700I GAANNNNTTC 3 cut(s) 687, 3162, 3957
AspA2I CCTAGG 1 cut(s) 5699
AspLEI GCGC 6 cut(s) 18, 2556, 3400, 5151, 5184, 5717
AsuC2I CCSGG 2 cut(s) 1255, 5591
AsuHPI GGTGA 9 cut(s) 992, 1019, 1427, 3173, 3602, 4358, 4414, 5207, 5281
AsuII TTCGAA 3 cut(s) 631, 1898, 2587
AsuNHI GCTAGC 3 cut(s) 952, 2456, 4097
AvaI CYCGRG 2 cut(s) 602, 2004
AvaII GGWCC 4 cut(s) 1661, 2827, 3784, 3815
AvrII CCTAGG 1 cut(s) 5699
AxyI CCTNAGG 1 cut(s) 2217
BaeGI GKGCMC 4 cut(s) 406, 1971, 2710, 5711
BaeI ACNNNNGTAYC 2 cut(s) 5224, 5257
BalI TGGCCA 1 cut(s) 4128
BanI GGYRCC 1 cut(s) 1305
BanII GRGCYC 2 cut(s) 2047, 5711
BarI GAAGNNNNNNTAC 4 cut(s) 2078, 2110, 3046, 3078
BauI CACGAG 4 cut(s) 327, 3597, 5123, 5550
BbrPI CACGTG 1 cut(s) 3875
BbsI GAAGAC 4 cut(s) 690, 3165, 3315, 3687
Bbv12I GWGCWC 5 cut(s) 406, 753, 987, 1971, 5412
BceAI ACGGC 3 cut(s) 67, 222, 800
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 8 cut(s) 652, 1573, 3185, 3788, 3813, 3908, 5107, 5246
BciVI GTATCC 2 cut(s) 2065, 5252
BclI TGATCA 5 cut(s) 802, 1365, 2659, 4194, 5271
BcnI CCSGG 2 cut(s) 1255, 5591
BcoDI GTCTC 5 cut(s) 1812, 2896, 3214, 4055, 4542
BcuI ACTAGT 2 cut(s) 3674, 4064
BfmI CTRYAG 6 cut(s) 1056, 1566, 2469, 4372, 4587, 4828
BfoI RGCGCY 2 cut(s) 19, 3401
BfrI CTTAAG 1 cut(s) 4201
BfuAI ACCTGC 1 cut(s) 4211
BfuI GTATCC 2 cut(s) 2065, 5252
BglII AGATCT 2 cut(s) 3439, 4308
BlnI CCTAGG 1 cut(s) 5699
BmcAI AGTACT 1 cut(s) 4531
Bme18I GGWCC 4 cut(s) 1661, 2827, 3784, 3815
BmeRI GACNNNNNGTC 1 cut(s) 4359
BmeT110I CYCGRG 2 cut(s) 602, 2004
BmrI ACTGGG 1 cut(s) 2942
BmsI GCATC 7 cut(s) 313, 1174, 1779, 3347, 3583, 5136, 5137
BmtI GCTAGC 3 cut(s) 956, 2460, 4101
BmuI ACTGGG 1 cut(s) 2942
BpiI GAAGAC 4 cut(s) 690, 3165, 3315, 3687
BplI GAGNNNNNCTC 4 cut(s) 310, 342, 1280, 1312
Bpu10I CCTNAGC 4 cut(s) 507, 513, 813, 4001
Bpu14I TTCGAA 3 cut(s) 631, 1898, 2587
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 2 cut(s) 1255, 5591
BsaAI YACGTR 3 cut(s) 3875, 4241, 5684
BsaBI GATNNNNATC 3 cut(s) 2179, 3438, 3795
BsaHI GRCGYC 1 cut(s) 2954
BsaI GGTCTC 2 cut(s) 1812, 2896
BsaJI CCNNGG 9 cut(s) 238, 650, 1254, 1572, 1814, 2611, 2869, 5589, 5699
BsaWI WCCGGW 1 cut(s) 3390
BsaXI ACNNNNNCTCC 6 cut(s) 952, 982, 3002, 3032, 4295, 4325
Bse118I RCCGGY 2 cut(s) 1755, 2133
Bse1I ACTGG 9 cut(s) 250, 2434, 2948, 3770, 4024, 4789, 5039, 5197, 5470
Bse21I CCTNAGG 1 cut(s) 2217
Bse3DI GCAATG 4 cut(s) 1368, 1464, 4613, 5177
Bse8I GATNNNNATC 3 cut(s) 2179, 3438, 3795
BseBI CCWGG 8 cut(s) 652, 1573, 3185, 3788, 3813, 3908, 5107, 5246
BseDI CCNNGG 9 cut(s) 238, 650, 1254, 1572, 1814, 2611, 2869, 5589, 5699
BseGI GGATG 9 cut(s) 2430, 3034, 3338, 4129, 4297, 5151, 5369, 5437, 5474
BseJI GATNNNNATC 3 cut(s) 2179, 3438, 3795
BseMI GCAATG 4 cut(s) 1368, 1464, 4613, 5177
BseNI ACTGG 9 cut(s) 250, 2434, 2948, 3770, 4024, 4789, 5039, 5197, 5470
BseRI GAGGAG 3 cut(s) 1028, 2234, 3729
BseSI GKGCMC 4 cut(s) 406, 1971, 2710, 5711
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 5 cut(s) 609, 3087, 4644, 4788, 5349
Bsh1236I CGCG 3 cut(s) 5151, 5638, 5715
Bsh1285I CGRYCG 2 cut(s) 3477, 4489
BshNI GGYRCC 1 cut(s) 1305
BsiEI CGRYCG 2 cut(s) 3477, 4489
BsiHKAI GWGCWC 5 cut(s) 406, 753, 987, 1971, 5412
BsiHKCI CYCGRG 2 cut(s) 602, 2004
BsiSI CCGG 6 cut(s) 66, 1254, 1756, 2134, 3391, 5591
BslFI GGGAC 3 cut(s) 2262, 2384, 2840
BsmAI GTCTC 5 cut(s) 1812, 2896, 3214, 4055, 4542
BsmFI GGGAC 3 cut(s) 2262, 2384, 2840
Bso31I GGTCTC 2 cut(s) 1812, 2896
BsoBI CYCGRG 2 cut(s) 602, 2004
Bsp119I TTCGAA 3 cut(s) 631, 1898, 2587
Bsp120I GGGCCC 1 cut(s) 5707
Bsp1286I GDGCHC 8 cut(s) 406, 753, 987, 1971, 2047, 2710, 5412, 5711
Bsp19I CCATGG 2 cut(s) 2611, 2869
BspACI CCGC 9 cut(s) 35, 261, 832, 3328, 3503, 3734, 3819, 5151, 5713
BspFNI CGCG 3 cut(s) 5151, 5638, 5715
BspHI TCATGA 2 cut(s) 591, 4261
BspMAI CTGCAG 1 cut(s) 1570
BspMI ACCTGC 1 cut(s) 4211
BspOI GCTAGC 3 cut(s) 956, 2460, 4101
BspT104I TTCGAA 3 cut(s) 631, 1898, 2587
BspT107I GGYRCC 1 cut(s) 1305
BspTI CTTAAG 1 cut(s) 4201
BspTNI GGTCTC 2 cut(s) 1812, 2896
BsrDI GCAATG 4 cut(s) 1368, 1464, 4613, 5177
BsrFI RCCGGY 2 cut(s) 1755, 2133
BsrI ACTGG 9 cut(s) 250, 2434, 2948, 3770, 4024, 4789, 5039, 5197, 5470
BssAI RCCGGY 2 cut(s) 1755, 2133
BssECI CCNNGG 9 cut(s) 238, 650, 1254, 1572, 1814, 2611, 2869, 5589, 5699
BssNAI GTATAC 1 cut(s) 1869
BssNI GRCGYC 1 cut(s) 2954
BssSI CACGAG 4 cut(s) 327, 3597, 5123, 5550
BssT1I CCWWGG 3 cut(s) 2611, 2869, 5699
Bst1107I GTATAC 1 cut(s) 1869
Bst2BI CACGAG 4 cut(s) 327, 3597, 5123, 5550
Bst2UI CCWGG 8 cut(s) 652, 1573, 3185, 3788, 3813, 3908, 5107, 5246
Bst6I CTCTTC 7 cut(s) 1005, 1825, 2006, 2352, 3312, 3645, 4566
BstACI GRCGYC 1 cut(s) 2954
BstAFI CTTAAG 1 cut(s) 4201
BstAPI GCANNNNNTGC 2 cut(s) 173, 2318
BstBAI YACGTR 3 cut(s) 3875, 4241, 5684
BstBI TTCGAA 3 cut(s) 631, 1898, 2587
BstDSI CCRYGG 3 cut(s) 238, 2611, 2869
BstENI CCTNNNNNAGG 1 cut(s) 1531
BstF5I GGATG 9 cut(s) 2430, 3034, 3338, 4129, 4297, 5151, 5369, 5437, 5474
BstFNI CGCG 3 cut(s) 5151, 5638, 5715
BstH2I RGCGCY 2 cut(s) 19, 3401
BstHHI GCGC 6 cut(s) 18, 2556, 3400, 5151, 5184, 5717
BstMAI GTCTC 5 cut(s) 1812, 2896, 3214, 4055, 4542
BstMCI CGRYCG 2 cut(s) 3477, 4489
BstNI CCWGG 8 cut(s) 652, 1573, 3185, 3788, 3813, 3908, 5107, 5246
BstNSI RCATGY 4 cut(s) 1522, 1720, 2809, 3097
BstSFI CTRYAG 6 cut(s) 1056, 1566, 2469, 4372, 4587, 4828
BstSLI GKGCMC 4 cut(s) 406, 1971, 2710, 5711
BstUI CGCG 3 cut(s) 5151, 5638, 5715
BstV2I GAAGAC 4 cut(s) 690, 3165, 3315, 3687
BstX2I RGATCY 7 cut(s) 220, 2283, 2362, 2463, 2982, 3439, 4308
BstXI CCANNNNNNTGG 5 cut(s) 3249, 3569, 3697, 4901, 5469
BstYI RGATCY 7 cut(s) 220, 2283, 2362, 2463, 2982, 3439, 4308
BstZ17I GTATAC 1 cut(s) 1869
Bsu36I CCTNAGG 1 cut(s) 2217
BsuI GTATCC 2 cut(s) 2065, 5252
BtgI CCRYGG 3 cut(s) 238, 2611, 2869
BtsCI GGATG 9 cut(s) 2430, 3034, 3338, 4129, 4297, 5151, 5369, 5437, 5474
BtsI GCAGTG 2 cut(s) 2183, 4030
BveI ACCTGC 1 cut(s) 4211
CaiI CAGNNNCTG 2 cut(s) 476, 4357
CciI TCATGA 2 cut(s) 591, 4261
CfoI GCGC 6 cut(s) 18, 2556, 3400, 5151, 5184, 5717
Cfr10I RCCGGY 2 cut(s) 1755, 2133
CseI GACGC 1 cut(s) 2962
Csp6I GTAC 9 cut(s) 824, 3282, 3371, 4530, 4656, 4725, 4774, 4891, 4946
CviQI GTAC 9 cut(s) 824, 3282, 3371, 4530, 4656, 4725, 4774, 4891, 4946
DraI TTTAAA 2 cut(s) 256, 1426
DraIII CACNNNGTG 1 cut(s) 4667
DrdI GACNNNNNNGTC 1 cut(s) 1418
DriI GACNNNNNGTC 1 cut(s) 4359
DseDI GACNNNNNNGTC 1 cut(s) 1418
EaeI YGGCCR 3 cut(s) 235, 653, 4126
Eam1104I CTCTTC 7 cut(s) 1005, 1825, 2006, 2352, 3312, 3645, 4566
Eam1105I GACNNNNNGTC 1 cut(s) 4359
EarI CTCTTC 7 cut(s) 1005, 1825, 2006, 2352, 3312, 3645, 4566
EciI GGCGGA 1 cut(s) 3317
Eco130I CCWWGG 3 cut(s) 2611, 2869, 5699
Eco147I AGGCCT 3 cut(s) 1019, 1933, 3413
Eco24I GRGCYC 2 cut(s) 2047, 5711
Eco31I GGTCTC 2 cut(s) 1812, 2896
Eco32I GATATC 1 cut(s) 599
Eco47I GGWCC 4 cut(s) 1661, 2827, 3784, 3815
Eco47III AGCGCT 1 cut(s) 3399
Eco57I CTGAAG 4 cut(s) 686, 2914, 3306, 3392
Eco72I CACGTG 1 cut(s) 3875
Eco81I CCTNAGG 1 cut(s) 2217
Eco88I CYCGRG 2 cut(s) 602, 2004
EcoNI CCTNNNNNAGG 1 cut(s) 1531
EcoO109I RGGNCCY 4 cut(s) 2827, 5696, 5707, 5708
EcoRI GAATTC 1 cut(s) 5627
EcoRII CCWGG 8 cut(s) 650, 1571, 3183, 3786, 3811, 3906, 5105, 5244
EcoRV GATATC 1 cut(s) 599
EcoT14I CCWWGG 3 cut(s) 2611, 2869, 5699
EcoT22I ATGCAT 3 cut(s) 199, 1603, 1842
EcoT38I GRGCYC 2 cut(s) 2047, 5711
ErhI CCWWGG 3 cut(s) 2611, 2869, 5699
FaqI GGGAC 3 cut(s) 2262, 2384, 2840
FauI CCCGC 2 cut(s) 3826, 5720
FauNDI CATATG 4 cut(s) 927, 2881, 3547, 5418
FbaI TGATCA 5 cut(s) 802, 1365, 2659, 4194, 5271
FblI GTMKAC 3 cut(s) 1868, 3711, 5193
FokI GGATG 9 cut(s) 2417, 3041, 3325, 4116, 4304, 5158, 5376, 5444, 5481
FriOI GRGCYC 2 cut(s) 2047, 5711
GlaI GCGC 6 cut(s) 17, 2555, 3399, 5150, 5183, 5716
GsaI CCCAGC 1 cut(s) 960
HaeII RGCGCY 2 cut(s) 19, 3401
HapII CCGG 6 cut(s) 66, 1254, 1756, 2134, 3391, 5591
HgaI GACGC 1 cut(s) 2962
HhaI GCGC 6 cut(s) 18, 2556, 3400, 5151, 5184, 5717
Hin1I GRCGYC 1 cut(s) 2954
Hin6I GCGC 6 cut(s) 16, 2554, 3398, 5149, 5182, 5715
HinP1I GCGC 6 cut(s) 16, 2554, 3398, 5149, 5182, 5715
HincII GTYRAC 3 cut(s) 1225, 3532, 4455
HindII GTYRAC 3 cut(s) 1225, 3532, 4455
HindIII AAGCTT 5 cut(s) 375, 1466, 3806, 4182, 5493
HpaI GTTAAC 1 cut(s) 4455
HpaII CCGG 6 cut(s) 66, 1254, 1756, 2134, 3391, 5591
HphI GGTGA 9 cut(s) 992, 1019, 1427, 3173, 3602, 4358, 4414, 5207, 5281
Hpy99I CGWCG 1 cut(s) 5322
HpyCH4IV ACGT 6 cut(s) 274, 2620, 3162, 3874, 4240, 5683
HpySE526I ACGT 6 cut(s) 274, 2620, 3162, 3874, 4240, 5683
Hsp92I GRCGYC 1 cut(s) 2954
HspAI GCGC 6 cut(s) 16, 2554, 3398, 5149, 5182, 5715
KroI GCCGGC 2 cut(s) 1755, 2133
KroNI GCCGGC 2 cut(s) 1757, 2135
Ksp22I TGATCA 5 cut(s) 802, 1365, 2659, 4194, 5271
KspAI GTTAAC 1 cut(s) 4455
LmnI GCTCC 8 cut(s) 130, 748, 1077, 1335, 2050, 3742, 4006, 4987
LweI GCATC 7 cut(s) 313, 1174, 1779, 3347, 3583, 5136, 5137
MaeII ACGT 6 cut(s) 274, 2620, 3162, 3874, 4240, 5683
MaeIII GTNAC 9 cut(s) 1036, 1591, 1913, 2015, 2818, 2950, 4072, 4252, 4364
MfeI CAATTG 3 cut(s) 2049, 3753, 4691
MflI RGATCY 7 cut(s) 220, 2283, 2362, 2463, 2982, 3439, 4308
MhlI GDGCHC 8 cut(s) 406, 753, 987, 1971, 2047, 2710, 5412, 5711
MlsI TGGCCA 1 cut(s) 4128
MluNI TGGCCA 1 cut(s) 4128
MlyI GAGTC 5 cut(s) 49, 2146, 3026, 4369, 4726
MmeI TCCRAC 7 cut(s) 66, 1589, 1807, 3099, 3767, 4010, 4945
Mox20I TGGCCA 1 cut(s) 4128
Mph1103I ATGCAT 3 cut(s) 199, 1603, 1842
MroNI GCCGGC 2 cut(s) 1755, 2133
MroXI GAANNNNTTC 3 cut(s) 687, 3162, 3957
MscI TGGCCA 1 cut(s) 4128
MslI CAYNNNNRTG 4 cut(s) 1845, 4134, 4764, 5417
Msp20I TGGCCA 1 cut(s) 4128
MspA1I CMGCKG 3 cut(s) 832, 1565, 5309
MspCI CTTAAG 1 cut(s) 4201
MspI CCGG 6 cut(s) 66, 1254, 1756, 2134, 3391, 5591
MunI CAATTG 3 cut(s) 2049, 3753, 4691
MvaI CCWGG 8 cut(s) 652, 1573, 3185, 3788, 3813, 3908, 5107, 5246
MvnI CGCG 3 cut(s) 5151, 5638, 5715
NaeI GCCGGC 2 cut(s) 1757, 2135
NciI CCSGG 2 cut(s) 1255, 5591
NcoI CCATGG 2 cut(s) 2611, 2869
NdeI CATATG 4 cut(s) 927, 2881, 3547, 5418
NgoMIV GCCGGC 2 cut(s) 1755, 2133
NheI GCTAGC 3 cut(s) 952, 2456, 4097
NmeAIII GCCGAG 1 cut(s) 1795
NmuCI GTSAC 6 cut(s) 1591, 2818, 2950, 4072, 4252, 4364
NsiI ATGCAT 3 cut(s) 199, 1603, 1842
NspI RCATGY 4 cut(s) 1522, 1720, 2809, 3097
NspV TTCGAA 3 cut(s) 631, 1898, 2587
PaeR7I CTCGAG 1 cut(s) 2004
PagI TCATGA 2 cut(s) 591, 4261
PceI AGGCCT 3 cut(s) 1019, 1933, 3413
PciI ACATGT 1 cut(s) 2805
PcsI WCGNNNNNNNCGW 1 cut(s) 4543
PdiI GCCGGC 2 cut(s) 1757, 2135
PdmI GAANNNNTTC 3 cut(s) 687, 3162, 3957
PflMI CCANNNNNTGG 1 cut(s) 1001
PfoI TCCNGGA 1 cut(s) 3811
Ple19I CGATCG 1 cut(s) 3477
PleI GAGTC 5 cut(s) 49, 2146, 3025, 4368, 4726
PmaCI CACGTG 1 cut(s) 3875
PmlI CACGTG 1 cut(s) 3875
PpsI GAGTC 5 cut(s) 49, 2146, 3025, 4368, 4726
Ppu21I YACGTR 3 cut(s) 3875, 4241, 5684
PpuMI RGGWCCY 1 cut(s) 2827
PscI ACATGT 1 cut(s) 2805
PshBI ATTAAT 1 cut(s) 4679
PsiI TTATAA 1 cut(s) 2636
Psp5II RGGWCCY 1 cut(s) 2827
Psp6I CCWGG 8 cut(s) 650, 1571, 3183, 3786, 3811, 3906, 5105, 5244
PspCI CACGTG 1 cut(s) 3875
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 8 cut(s) 650, 1571, 3183, 3786, 3811, 3906, 5105, 5244
PspOMI GGGCCC 1 cut(s) 5707
PspPPI RGGWCCY 1 cut(s) 2827
PspXI VCTCGAGB 1 cut(s) 2004
PstI CTGCAG 1 cut(s) 1570
PstNI CAGNNNCTG 2 cut(s) 476, 4357
PsuI RGATCY 7 cut(s) 220, 2283, 2362, 2463, 2982, 3439, 4308
PvuI CGATCG 1 cut(s) 3477
PvuII CAGCTG 2 cut(s) 1565, 5309
RsaI GTAC 9 cut(s) 825, 3283, 3372, 4531, 4657, 4726, 4775, 4892, 4947
RsaNI GTAC 9 cut(s) 824, 3282, 3371, 4530, 4656, 4725, 4774, 4891, 4946
RseI CAYNNNNRTG 4 cut(s) 1845, 4134, 4764, 5417
ScaI AGTACT 1 cut(s) 4531
SchI GAGTC 5 cut(s) 49, 2146, 3026, 4369, 4726
SduI GDGCHC 8 cut(s) 406, 753, 987, 1971, 2047, 2710, 5412, 5711
SfaNI GCATC 7 cut(s) 313, 1174, 1779, 3347, 3583, 5136, 5137
SfcI CTRYAG 6 cut(s) 1056, 1566, 2469, 4372, 4587, 4828
Sfr274I CTCGAG 1 cut(s) 2004
SfuI TTCGAA 3 cut(s) 631, 1898, 2587
SinI GGWCC 4 cut(s) 1661, 2827, 3784, 3815
SlaI CTCGAG 1 cut(s) 2004
SmiMI CAYNNNNRTG 4 cut(s) 1845, 4134, 4764, 5417
SmlI CTYRAG 3 cut(s) 886, 2004, 4201
SmoI CTYRAG 3 cut(s) 886, 2004, 4201
SpeI ACTAGT 2 cut(s) 3674, 4064
SseBI AGGCCT 3 cut(s) 1019, 1933, 3413
SsiI CCGC 9 cut(s) 35, 261, 832, 3328, 3503, 3734, 3819, 5151, 5713
SspI AATATT 1 cut(s) 553
StuI AGGCCT 3 cut(s) 1019, 1933, 3413
StyI CCWWGG 3 cut(s) 2611, 2869, 5699
TaiI ACGT 6 cut(s) 277, 2623, 3165, 3877, 4243, 5686
TatI WGTACW 3 cut(s) 823, 4529, 4773
TauI GCSGC 2 cut(s) 3505, 3737
TseFI GTSAC 6 cut(s) 1591, 2818, 2950, 4072, 4252, 4364
Tsp45I GTSAC 6 cut(s) 1591, 2818, 2950, 4072, 4252, 4364
TspGWI ACGGA 5 cut(s) 67, 125, 3023, 4643, 4758
Van91I CCANNNNNTGG 1 cut(s) 1001
Vha464I CTTAAG 1 cut(s) 4201
VneI GTGCAC 2 cut(s) 402, 1967
VpaK11BI GGWCC 4 cut(s) 1661, 2827, 3784, 3815
VspI ATTAAT 1 cut(s) 4679
XagI CCTNNNNNAGG 1 cut(s) 1531
XapI RAATTY 9 cut(s) 2079, 2273, 2315, 2643, 2941, 3539, 3956, 4814, 5627
XbaI TCTAGA 2 cut(s) 277, 2155
XceI RCATGY 4 cut(s) 1522, 1720, 2809, 3097
XcmI CCANNNNNNNNNTGG 1 cut(s) 3181
XhoI CTCGAG 1 cut(s) 2004
XmaJI CCTAGG 1 cut(s) 5699
XmiI GTMKAC 3 cut(s) 1868, 3711, 5193
XmnI GAANNNNTTC 3 cut(s) 687, 3162, 3957
ZrmI AGTACT 1 cut(s) 4531
Zsp2I ATGCAT 3 cut(s) 199, 1603, 1842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.