RLG00000030805

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002059
Physical Location & Seq
Reverse (-)
67816743 .. 67820714
3972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030805

Sequence Viewer

Length: 1398 bp
ATGGCATATAGAAGGGGCTTCGACCAGCCGCAACCGCAGAGGCGGATTCTGCGGACTCAGACTGCTGGAAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGTTCCTATTCTCAGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGGGTTGCCTATCTCTGTCGCTTCTTTGCATTTTGGAAAGCTGACAGACTAGATCCAGCATCTGGTGGCTCTGGTGTTCGCCAGTTTAAAACTGCATTTCTTCAACGTCTAGAGAGGGAAAAAGAAACAACATTGGCAGGACGAGCAGAGAGTGATGCTTGTGAAATGCAGAGTTTATATCAAGATTACTACCAAAAATACATTCAAGCTTTGCACAGTACTGCTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTGTTTTGTATGAGGTTTTGATGGCTGTCAATCGGACAGAGTGTGTTGAAGTCGCTGGAGAGTTTTTGGAAGCCCACACTAAGGTTGAAGAAAAACAACAGATATATCTACCTTACAATATTCTTCCTCTTGATCCTAATAGTCATCAGGCAATCATGAGATGTCCCGAGACTCATGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGTCGAAGGACTACAAAAAGAAAGAAGATGCAGACATTGTTGATTGGCTTCAAGCTATGTTTGGTTTTCAGAAGGATAATATCGCCAATCAAAGGGAGCACTTGATTCTTTTGATCGCTAGTGTTCACTTACGGCAATTACCAAAATCTGATCAGCATACAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGAATCATATGGCATTTGAATTATATGGAATGCTAGCTGGGAATGTTAATCCAATGACGGGTGAGCACATAAAGCCCGCCTATGGTGGTGAAGAGGGGGCCTTGTTGAGGAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCATTGGAGAAATTATGATGACTTGAATGAATACTTTCGGAAAGTTGATTGTTTTAGGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTGGTAAAGAGCTTGAAGTTGACAGAGAAGGAGTGGAGAGTTCGAGGGCTAGGGAGGAGATGGAAAAGACTTTTGAGGAACAAGCAAACACAGCCAGTGCTATGGTGGTTGCAGTGGAGGAGGCAGCGGCAGGTGTGACGGGGAAGCTCCGGCTAGAGAGGAGGAAGGGTCGAAGTTCAAGCGCACACGCCATGCACTTTCGCTGGCCCCTGGAAGGCTGGAAGAGGATAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

466

Amino Acids

52.92

Weight (kDa)

6.94

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 169 1.3e-13 Vta1 like
FKS1_dom1 PF14288 291 - 377 2.3e-17 1,3-beta-glucan synthase subunit FKS1, domain-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1283
Acc36I ACCTGC 1 cut(s) 1283
AccB7I CCANNNNNTGG 1 cut(s) 230
AciI CCGC 7 cut(s) 29, 35, 43, 52, 821, 954, 1289
AclWI GGATC 2 cut(s) 215, 566
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 7 cut(s) 230, 520, 747, 935, 959, 984, 1376
AgsI TTSAA 9 cut(s) 272, 374, 488, 527, 707, 896, 1087, 1178, 1341
AjnI CCWGG 3 cut(s) 653, 1162, 1371
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 6 cut(s) 209, 377, 710, 914, 1174, 1309
AluI AGCT 6 cut(s) 209, 377, 710, 914, 1174, 1309
Alw21I GWGCWC 3 cut(s) 412, 756, 945
Alw26I GTCTC 1 cut(s) 602
Alw44I GTGCAC 1 cut(s) 408
AlwI GGATC 2 cut(s) 215, 566
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 3 cut(s) 975, 1127, 1367
ApaLI GTGCAC 1 cut(s) 408
ApeKI GCWGC 4 cut(s) 437, 818, 867, 1286
Asp700I GAANNNNTTC 1 cut(s) 1095
AspLEI GCGC 1 cut(s) 1346
AspS9I GGNCC 2 cut(s) 975, 1368
AsuHPI GGTGA 2 cut(s) 950, 977
AsuNHI GCTAGC 1 cut(s) 910
AvaI CYCGRG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 412
Bbv12I GWGCWC 3 cut(s) 412, 756, 945
BbvI GCAGC 4 cut(s) 424, 830, 854, 1298
BccI CCATC 2 cut(s) 454, 1216
BceAI ACGGC 2 cut(s) 67, 803
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 3 cut(s) 655, 1164, 1373
BclI TGATCA 1 cut(s) 805
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 8 cut(s) 159, 218, 278, 774, 911, 1121, 1212, 1316
BfmI CTRYAG 1 cut(s) 1014
BfuAI ACCTGC 1 cut(s) 1283
BisI GCNGC 6 cut(s) 29, 438, 819, 868, 1287, 1290
BlsI GCNGC 6 cut(s) 30, 439, 820, 869, 1288, 1291
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 3 cut(s) 655, 1164, 1373
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 975, 1368
BmiI GGNNCC 2 cut(s) 976, 1370
BmrFI CCNGG 3 cut(s) 655, 1164, 1373
BmsI GCATC 4 cut(s) 236, 313, 673, 1132
BmtI GCTAGC 1 cut(s) 914
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 896
BsaJI CCNNGG 2 cut(s) 653, 1371
BsaXI ACNNNNNCTCC 2 cut(s) 1315, 1345
Bsc4I CCNNNNNNNGG 7 cut(s) 230, 520, 747, 935, 959, 984, 1376
Bse1I ACTGG 2 cut(s) 250, 1257
BseBI CCWGG 3 cut(s) 655, 1164, 1373
BseDI CCNNGG 2 cut(s) 653, 1371
BseLI CCNNNNNNNGG 7 cut(s) 230, 520, 747, 935, 959, 984, 1376
BseMII CTCAG 2 cut(s) 71, 148
BseNI ACTGG 2 cut(s) 250, 1257
BseRI GAGGAG 3 cut(s) 1232, 1295, 1336
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 4 cut(s) 424, 830, 854, 1298
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 3 cut(s) 977, 1129, 1369
BsiHKAI GWGCWC 3 cut(s) 412, 756, 945
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 1 cut(s) 1312
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 7 cut(s) 230, 520, 747, 935, 959, 984, 1376
BsmAI GTCTC 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 3 cut(s) 169, 912, 1057
BsnI GGCC 3 cut(s) 977, 1129, 1369
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 3 cut(s) 412, 756, 945
Bsp143I GATC 5 cut(s) 220, 403, 571, 768, 805
BspACI CCGC 7 cut(s) 29, 35, 43, 52, 821, 954, 1289
BspANI GGCC 3 cut(s) 977, 1129, 1369
BspCNI CTCAG 2 cut(s) 70, 147
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 976, 1370
BspMI ACCTGC 1 cut(s) 1283
BspOI GCTAGC 1 cut(s) 914
BspPI GGATC 2 cut(s) 215, 566
BsrI ACTGG 2 cut(s) 250, 1257
BssECI CCNNGG 2 cut(s) 653, 1371
BssMI GATC 5 cut(s) 220, 403, 571, 768, 805
Bst2UI CCWGG 3 cut(s) 655, 1164, 1373
Bst4CI ACNGT 2 cut(s) 386, 622
Bst6I CTCTTC 2 cut(s) 963, 1379
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 5 cut(s) 912, 954, 1127, 1137, 1367
BstDEI CTNAG 3 cut(s) 57, 134, 519
BstENI CCTNNNNNAGG 1 cut(s) 982
BstHHI GCGC 1 cut(s) 1346
BstKTI GATC 5 cut(s) 223, 406, 574, 771, 808
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 5 cut(s) 220, 403, 571, 768, 805
BstMWI GCNNNNNNNGC 8 cut(s) 34, 49, 173, 311, 949, 1025, 1126, 1253
BstNI CCWGG 3 cut(s) 655, 1164, 1373
BstSCI CCNGG 3 cut(s) 653, 1162, 1371
BstSFI CTRYAG 1 cut(s) 1014
BstSLI GKGCMC 1 cut(s) 412
BstV1I GCAGC 4 cut(s) 424, 830, 854, 1298
BstX2I RGATCY 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 1264
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 3 cut(s) 977, 1129, 1369
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 3 cut(s) 142, 1264, 1281
BveI ACCTGC 1 cut(s) 1283
Cac8I GCNNGC 5 cut(s) 912, 954, 1127, 1137, 1367
CaiI CAGNNNCTG 1 cut(s) 230
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 1346
Cfr13I GGNCC 2 cut(s) 975, 1368
Csp6I GTAC 1 cut(s) 387
CviAII CATG 4 cut(s) 595, 614, 836, 1354
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 3 cut(s) 57, 134, 519
DpnI GATC 5 cut(s) 222, 405, 573, 770, 807
DpnII GATC 5 cut(s) 220, 403, 571, 768, 805
DraI TTTAAA 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 963, 1379
EarI CTCTTC 2 cut(s) 963, 1379
EciI GGCGGA 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 982
EcoO109I RGGNCCY 1 cut(s) 975
EcoRII CCWGG 3 cut(s) 653, 1162, 1371
FaeI CATG 4 cut(s) 598, 617, 839, 1357
FaqI GGGAC 1 cut(s) 588
FatI CATG 4 cut(s) 594, 613, 835, 1353
FauI CCCGC 1 cut(s) 961
FauNDI CATATG 1 cut(s) 885
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 6 cut(s) 29, 438, 819, 868, 1287, 1290
Fsp4HI GCNGC 6 cut(s) 29, 438, 819, 868, 1287, 1290
FspBI CTAG 8 cut(s) 159, 218, 278, 774, 911, 1121, 1212, 1316
GlaI GCGC 1 cut(s) 1345
GluI GCNGC 6 cut(s) 29, 438, 819, 868, 1287, 1290
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 3 cut(s) 977, 1129, 1369
HapII CCGG 1 cut(s) 1312
HhaI GCGC 1 cut(s) 1346
Hin1II CATG 4 cut(s) 598, 617, 839, 1357
Hin6I GCGC 1 cut(s) 1344
HinP1I GCGC 1 cut(s) 1344
HincII GTYRAC 1 cut(s) 1183
HindII GTYRAC 1 cut(s) 1183
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 6 cut(s) 46, 55, 155, 610, 760, 880
HpaII CCGG 1 cut(s) 1312
HphI GGTGA 2 cut(s) 950, 977
Hpy166II GTNNAC 4 cut(s) 410, 650, 781, 1183
Hpy188I TCNGA 6 cut(s) 60, 474, 635, 726, 805, 1101
Hpy188III TCNNGA 5 cut(s) 278, 350, 569, 595, 605
Hpy8I GTNNAC 4 cut(s) 410, 650, 781, 1183
HpyAV CCTTC 7 cut(s) 6, 114, 655, 721, 1184, 1321, 1370
HpyCH4III ACNGT 2 cut(s) 386, 622
HpyCH4IV ACGT 1 cut(s) 274
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 49, 173, 311, 949, 1025, 1126, 1253
HpyF3I CTNAG 3 cut(s) 57, 134, 519
HpySE526I ACGT 1 cut(s) 274
Hsp92II CATG 4 cut(s) 598, 617, 839, 1357
HspAI GCGC 1 cut(s) 1344
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 5 cut(s) 220, 403, 571, 768, 805
LmnI GCTCC 3 cut(s) 751, 1035, 1314
Lsp1109I GCAGC 4 cut(s) 424, 830, 854, 1298
LweI GCATC 4 cut(s) 236, 313, 673, 1132
MaeI CTAG 8 cut(s) 159, 218, 278, 774, 911, 1121, 1212, 1316
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 2 cut(s) 994, 1297
MalI GATC 5 cut(s) 222, 405, 573, 770, 807
MboI GATC 5 cut(s) 220, 403, 571, 768, 805
MboII GAAGA 8 cut(s) 99, 260, 539, 554, 692, 840, 980, 1396
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 3 cut(s) 412, 756, 945
MluCI AATT 5 cut(s) 791, 825, 896, 1072, 1392
MlyI GAGTC 2 cut(s) 49, 604
MmeI TCCRAC 1 cut(s) 66
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 2 cut(s) 255, 924
MspA1I CMGCKG 2 cut(s) 821, 1289
MspI CCGG 1 cut(s) 1312
MspR9I CCNGG 3 cut(s) 655, 1164, 1373
Mva1269I GAATGC 3 cut(s) 169, 912, 1057
MvaI CCWGG 3 cut(s) 655, 1164, 1373
MwoI GCNNNNNNNGC 8 cut(s) 34, 49, 173, 311, 949, 1025, 1126, 1253
NdeI CATATG 1 cut(s) 885
NdeII GATC 5 cut(s) 220, 403, 571, 768, 805
NheI GCTAGC 1 cut(s) 910
NlaIII CATG 4 cut(s) 598, 617, 839, 1357
NlaIV GGNNCC 2 cut(s) 976, 1370
NmuCI GTSAC 1 cut(s) 1297
PagI TCATGA 1 cut(s) 594
PaqCI CACCTGC 1 cut(s) 1283
PctI GAATGC 3 cut(s) 169, 912, 1057
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 4 cut(s) 46, 155, 760, 880
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 6 cut(s) 30, 439, 820, 869, 1288, 1291
PleI GAGTC 2 cut(s) 49, 604
PpsI GAGTC 2 cut(s) 49, 604
Psp6I CCWGG 3 cut(s) 653, 1162, 1371
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 3 cut(s) 653, 1162, 1371
PspN4I GGNNCC 2 cut(s) 976, 1370
PspPI GGNCC 2 cut(s) 975, 1368
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 255, 924
SatI GCNGC 6 cut(s) 29, 438, 819, 868, 1287, 1290
Sau3AI GATC 5 cut(s) 220, 403, 571, 768, 805
Sau96I GGNCC 2 cut(s) 975, 1368
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 2 cut(s) 49, 604
ScrFI CCNGG 3 cut(s) 655, 1164, 1373
SduI GDGCHC 3 cut(s) 412, 756, 945
SfaNI GCATC 4 cut(s) 236, 313, 673, 1132
SfcI CTRYAG 1 cut(s) 1014
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
Sse9I AATT 5 cut(s) 791, 825, 896, 1072, 1392
SsiI CCGC 7 cut(s) 29, 35, 43, 52, 821, 954, 1289
SspI AATATT 1 cut(s) 559
SspMI CTAG 8 cut(s) 159, 218, 278, 774, 911, 1121, 1212, 1316
StyD4I CCNGG 3 cut(s) 653, 1162, 1371
TaaI ACNGT 2 cut(s) 386, 622
TaiI ACGT 1 cut(s) 277
TaqI TCGA 5 cut(s) 21, 153, 659, 1205, 1333
TasI AATT 5 cut(s) 791, 825, 896, 1072, 1392
TatI WGTACW 1 cut(s) 386
TauI GCSGC 2 cut(s) 31, 1292
TfiI GAWTC 4 cut(s) 46, 155, 760, 880
Tru1I TTAA 2 cut(s) 255, 924
Tru9I TTAA 2 cut(s) 255, 924
TscAI CASTG 3 cut(s) 142, 1264, 1281
TseFI GTSAC 1 cut(s) 1297
TseI GCWGC 4 cut(s) 437, 818, 867, 1286
Tsp45I GTSAC 1 cut(s) 1297
TspDTI ATGAA 2 cut(s) 162, 1104
TspRI CASTG 3 cut(s) 142, 1264, 1281
Van91I CCANNNNNTGG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 408
XagI CCTNNNNNAGG 1 cut(s) 982
XbaI TCTAGA 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 8 cut(s) 159, 218, 278, 774, 911, 1121, 1212, 1316
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.