AT5G36870

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002059
Physical Location & Seq
Forward (+)
14518008 .. 14534060
16053 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G36870.2

Sequence Viewer

Length: 5616 bp
ATGAATCAGCCCAACAGAGGGCAGATTCTCCAAACTGTGTTTAGCCACTTTTTCCCAGTAGCTAGTCCTGACAGTGAGCTGGTTCCATCTTCTCTTCATGAGGATATTACTCCCATCCTTCGTGTAGCCAAAGACGTAGAGGATACTAATCCCCGGAGTTTATTTCTTCAAGATCTTGACATCAAATCTGTGGATGATTCTATAAATATACTGTCAGGTCATTCACATGCGCTAGATAAGGCAAACGAGCTTGATCCCACATCAAGTGGACGTGATGTTCGCCAGTTCAAGAATACAATTCTTCAATGGTTAGAAAAGAATAATGAAAGTACTCTAAAGGCAAGACAGAAGAGTAGTGATGCACATGAAATGCAAAGTTTCTATCAACAATATGGTGACGAGGGCATCAATGATTTGCTAAATGCTGGTGCTGGCAGTTCTAGTTCTCAACGTACAAAAATATATCAAACTGCTGTTGTTCTCTATGATGTCTTGGATGCTGTTCATCGGAAAGCTAATATCAAAGTGGCTGCTAAGATTTTGGAATCCCATGCCGAGGTGGAAGCAAAAAACAAAATCTATGTGCCTTACAATATTCTTCCCCTCGATCCTGATAGTAAAAATCACGCTATGATGAGAGATCCTAAGATTGTAGCTGTATTAAAGGCAATTCGTTACACAAGTGACTTAACATGGCAGATAGGTCACAAGATCAATGACGATGAAGACGTGCTAGACTGGCTAAAAACTATGTTTCGCTTTCAGAAAGATAATGTTTCAAATCAAAGAGAGCACTTGATTTTGTTACTTGCTAATGTCCAAATGCGACAAACCCAGAGACAACCAAATTTGTTGGATGATCGAGCCTTGGATACAGTGATGGAGAAATTATTAGGAAATTACAATAAATGGTGTAATCATGTGGGCCTGGAAAGCAGCCTTAGGTTCCCTAAGGATAAGCAACAAAAGGTGGTACAACAGAGGAAACTACTATATACAGGACTCTATCTTTTAATATGGGGAGAAGCTGCAAACCTTAGATTCATGCCCGAGTGCCTTTGCTATATCTATCACCATATGGCGTTTGAGTTGTTTGAGATGTTGGAGAGTAAAGGTAGTAAGAAGAAATATAAGCCTAAAAACCCAACATATAGTGGTAAAGATGAAGATTTCTTGACGAAAGTAGTGACTCCTGTTTACAAGACAATAGCCGAGGAAGCAAAAAAAAGTGGCGAAGGAAAGCATTCTGAGTGGAGAAACTATGACGACTTGAACGAATACTTCTGGTCAAAACAATACCTTGATAAGTTAGGGTGGCCTATGAAAGCCAATGCTGATTTCTTTTGTAAGACATCTCAACAACTTGGTCTGAATAAAAGTGAGAAGAAACCAGATCTGGGAGATGGATGTGTCGGAAAAGTCAATTTTGTTGAGATACGCACCTTTTGGCATCTATTTAGAAGTTTTGACAGAATGTGGAGTTTTTATATCTTGTCTTTGCAGGCAATGATCATAATTGCTTGGAATGAAACCAGTGAATCTGGGGGTGCGGTCTTTCATAAGGTTTTGAGCGTCTTCATCACAGCTGCTAAATTAAACCTTTTCCAAGCTTTCCTTGATATAGCTCTGAGCTGGAAAGCAAGACATAGCATGTCAACACATGTGAGGCAAAGATACATCTTCAAGGCAGTGGCGGCTGCAGTATGGGTTTTACTCATGCCATTAACTTACGCATACAGCCACACCTCTATTTTCATTGTGGCTATTCTTATCTACTTATCCCCAAACATGCTCCCGGAAATGTTGCTCTTGATTCCTAGCATCCGACGTACACTTGAAAAGTCTGACTTTAGGCCAGTGAAATTGATAATGTGGTGGTCTCAGCCTGAACTGTATATAGGAAGAGGTATGCATGAAAGTGCATGGTCAATCTACAAGTACATGATGTTCTGGATTGTCTTGTTAACCTCAAAGCTTGCATTCAGTTATTATGTGGAGCAGATTAAGCCTCTTATGGGTCCAACCAAAGAAATTATGAGTGTTCCTATGCCAGGCTACTGGTTGCCCGAGTTTTTCCCTCATGTTAAGAACAATAGAGGTGTTGTGATCACACTCTGGTCACCTGTTATCCTTGTTTATTTCATGGACACTCAAATATGGTATGCTATAGTATCAACTCTGGTTGGAGGTTTATATGGAGCTTTTCGACACATCGGAGAGATCCAAACACTTGGAATGTTGAGGTCTAGATTTCAGTCCTTACCAGGAGCCTTTAATGCTTGTTTGATTCCAAATGAGAATACGAAAGAAAAAGGAATTAAATTGGCTTTCTCTCGCAAGTGCCACAAGATCCCAAATACTAATGGCAAGGAAGCAAAACAATTTTCTCAGATGTGGAACACCATAATTAATAGTTTTCGAGAAGAGGATCTGATTAGCAATAGGGAACTGGAACTCCTGCTGATGTCATGTTGGGCTTACCCTGATCTGGATTTTATCCGCTGGCCAATTTTTCTGTTGGCCAGTAAGATCCCAATAGCCGTGGATATTGCAAAAAAAAGGAATGGAAAACACCGTGAACTGAAAAATATACTGGCTGAGGACAATTGCATGAGTTGTGCTGTTAGAGAGTGTTATGCTTCTATTAAGAAACTCTTGAATACTCTGGTCACCGGAAATAGTGATTTAATGCTCATAACCACAGTTTTCACCATTATTGACACACATATAGAGAAAGATACACTCTTGACAGAGTTGAATTTGAGTGTTCTTCCTGATCTGCATGGCCACTTTGTTAAGCTAACTGAATATGTGCTGCAAAACAAAGACAAGGACAAGATTCAGATTGTTAATGTCTTGCTGAAAATATTAGAAATGGTGACAAAAGACATACTAAAGGAAGAGATCAAGAGGCTTCATCTTTTGCTTACTGTCAAAGAGTCGGCTATGGATGTTCCATCCAACTTGGAAGCGCGAAGGCGTCTTACATTCTTTTCAAACTCATTATTCATGGAAATGCCTGGTGCACCCAAAATCCAAAACATGCTTTCCTTTTCGGCACTAACACCATACTATTCAGAGGATGTCCTTTTCTCCACATTTGATCTGGAAAAGGAAAATGATGGAGTCTCAATACTGTTTTACTTGCAGAAAATATTTCCAGATGAATGGAAAAACTTTCTGGAACGAGTTAAATGTGGCACTGAGGAAGAACTCGACGCAATAGATTATTTGAAAGAGGAGATTCGTCTTTGGGCATCTTACAGGGGCCAAACACTGACCAAAACAGTGCGAGGCATGATGTATTACCAAAAGGCTTTGGAGCTTCAGGCCTTCTTTGATCTAGCTAATGAAAGAGAATTAATGAAAGGATACAAGAGTGCCGAAGCTTCAAGTAGTGGAAGTTCACTCTGGGCTGAGTGTCAAGCACTTGCTGATATTAAATTCACCTATGTGGTCGCATGCCAACAATACAGTATACATAAGAGATCTGGTGACCAACGTGCTAAAGACATATTGACGCTTATGACTACGTACCCATCTTTACGTGTTGCTTATATCGATGAGGTTGAGCAAACACATATATATAGTAAGGGGACAAGTGAAAATTTTTATTACTCAGCTCTTGTCAAAGCTGCTCCACAAACCTATTCTACCGATTCCTCTGATTCTGGTCATATGCTAGACCAGGTCATTTATCAGATTAAACTACCAGGACCTCCTATAATAGGAGAAGGAAAACCGGAAAATCAGAACAATGCTATCATTTTTACACGCGGAGAAGCGTTGCAGACAATAGACATGAACCAGGATTATTACATAGAAGAAGCTTTCAAAATGAGGAACTTGCTACAAGAATTTCTTGAAAAGAATGGAGGCGTGAGATATCCTACGATACTTGGACTAAGAGAGCATATTTTCACACGAAGTGTTTCTTGTCTTGCATGGTTTATGTCAAATCAGGAGCATAGCTTTGTTACTATTGGGCAGAGGGTGCTAGCTAATCCTTTGAAGGTACGATTTCATTACGGTCATCCAGATGTTTTTGATAGAGTATTTCACCTGACCAGAGGGGGTGTCTCCAAAGCTTCTAAAGTTATTAATCTAAGTGAAGACATATTTGCAGGTTTCAATTCTACTCTACGTGAAGGCACCGTGAGTCATCATGAATACATACAAGTTGGTAAAGGGAGAGATGTGGGACTCAACCAAATTTCAATGTTTGAGGCAAAGATAGCTAACGGAAGTGGTGAGCAAACTCTAAGTCGTGACCTATATAGACTAGGCCACCAATTTGATTTCTTCCGTATGCTCTCTTGCTATTTCACTACAGTTGGGTTCTATTTCTGTAGCATGTTAACCGTGCTTACGGTCTATGTATTTCTCTATGGACGGTTGTATCTCGTTCTAAGTGGGGTTGAAAAAGAATTGGGGAACAAGCCTATGATGATGGAAATTATCCTTGCTTCGCAGTCGTTCGTGCAGATTGTTTTTTTGATGGCCATGCCTATGATCATGGAGATTGGGCTTGAGAGGGGATTTTATGACGCCCTATTTGATTTTGTATTGATGCAGCTACAACTTGCTTCCGTATTTTTCACATTTCAACTTGGGACAAAATTTCATTACTACTGCAAGACATTGTTGCATGGAGGCGCAGAATACAGAGGTACTGGCCGTGGATTTGTGGTGTTCCATGCCAAATTTGCTGAAAACTACAGGTTTTACTCTCGTAGTCATTTTGTGAAGGCTACTGAGCTAGGGATTCTCCTACTCGTGTATCACATATTTGGCCCAACCTATATAGGGTTGTTCACTATTTCGATATGGTTCATGGTGGGTACATGGCTCTTTGCTCCTTTCTTATTCAACCCATCTGGTTTTGAGTGGCATGAAATTGTCGAAGACTGGGCAGATTGGAAAAAATGGATTGAATATGATAATGGGGGGATAGGTGTTCCTCCAGAGAAGAGCTGGGAATCATGGTGGGAGAAGGATATTGAACATCTTCAACACTCGGGAAAATGGGGTATCGTTGTTGAGATTTTTTTTGCATTACGCTTCTTCATATTTCAGTACGGACTTGTGTATCAACTCAGCGCCTTTAAAAATAAATATAGTAGTTTATGGGTTTTTGGGGCTTCATGGTTACTGATCTTAATCCTTTTACTCACTGTGACGGTTTTGGATTATGCAAGGCGACGGCTGGGAACAGAGTTCCAACTTTTATTCAGGATCATCAAGGTTTCCTTGTTTCTCGCATTTATGGCAATATTCATTACTCTCATGACTTGTCGTCTTATACTACCCCAAGACGTCTTCCTCTGCATGCTTGCATTAATCCCAACTGGATGGGGATTACTACTTATTGCACAATCTTGTAAGCCTCTGATTCAACAACCAGGGATATGGTCTTGGGTGATGACGTTGGCTTGGGTCTACGACCTCGTGATGGGTTCTCTCTTGTTTATTCCAATAGCGTTTATGGCCTGGTTCCCATTCATATCGGAGTTCCAAACGAGAATGCTCTTTAACCAAGCCTTTAGCAGAGGTCTACATATCTCACGTATTCTCAGTGGTCAGAGAAAACACCGCTCCTCCAAGAATAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1871

Amino Acids

216.14

Weight (kDa)

8.46

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 43 - 171 1.9e-09 Vta1 like
FKS1_dom1 PF14288 321 - 432 1.4e-40 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 828 - 959 6.6e-39 Callose synthase 1 domain
Glucan_synthase PF02364 977 - 1064 5.2e-26 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1072 - 1672 4e-196 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 5319
Acc36I ACCTGC 1 cut(s) 4115
AccB1I GGYRCC 1 cut(s) 4151
AccBSI CCGCTC 1 cut(s) 5595
AccI GTMKAC 3 cut(s) 3478, 5439, 5554
AccII CGCG 2 cut(s) 2974, 3777
AciI CCGC 5 cut(s) 1550, 1694, 2500, 3777, 5593
AclWI GGATC 8 cut(s) 248, 602, 635, 2215, 2344, 2436, 2524, 5243
AcoI YGGCCR 5 cut(s) 2504, 2520, 2785, 4500, 4675
AcsI RAATTY 8 cut(s) 847, 2758, 3443, 3607, 3857, 4212, 4619, 4703
AcuI CTGAAG 1 cut(s) 3311
AcyI GRCGYC 3 cut(s) 2980, 4548, 5316
AdeI CACNNNGTG 1 cut(s) 3929
AflIII ACRYGT 2 cut(s) 1660, 3547
AhdI GACNNNNNGTC 1 cut(s) 5295
AjiI CACGTC 2 cut(s) 272, 730
AjnI CCWGG 9 cut(s) 927, 2050, 2263, 3019, 3687, 3712, 3807, 5401, 5489
AjuI GAANNNNNNNTTGG 2 cut(s) 1322, 1354
AleI CACNNNNGTG 1 cut(s) 3452
AloI GAACNNNNNNTCC 4 cut(s) 261, 293, 2439, 2471
Alw21I GWGCWC 2 cut(s) 795, 3028
Alw26I GTCTC 4 cut(s) 832, 1884, 3133, 4084
Alw44I GTGCAC 1 cut(s) 3024
AlwI GGATC 8 cut(s) 248, 602, 635, 2215, 2344, 2436, 2524, 5243
Ama87I CYCGRG 3 cut(s) 1049, 2066, 5017
ApaLI GTGCAC 1 cut(s) 3024
ApeKI GCWGC 8 cut(s) 530, 936, 1028, 1586, 1697, 2815, 3635, 4573
ApoI RAATTY 8 cut(s) 847, 2758, 3443, 3607, 3857, 4212, 4619, 4703
ArsI GACNNNNNNTTYG 6 cut(s) 261, 293, 4249, 4281, 4538, 4570
AseI ATTAAT 4 cut(s) 2409, 3362, 4101, 5339
Asp700I GAANNNNTTC 3 cut(s) 1243, 3931, 5007
AspLEI GCGC 4 cut(s) 232, 2974, 4658, 5102
AspS9I GGNCC 5 cut(s) 925, 2018, 3268, 3716, 4793
AsuC2I CCSGG 2 cut(s) 154, 1796
AsuNHI GCTAGC 1 cut(s) 3997
AvaI CYCGRG 3 cut(s) 1049, 2066, 5017
AvaII GGWCC 2 cut(s) 2018, 3716
AxyI CCTNAGG 2 cut(s) 941, 951
BaeGI GKGCMC 1 cut(s) 3028
BaeI ACNNNNGTAYC 8 cut(s) 3364, 3397, 4382, 4415, 4763, 4796, 5072, 5105
BalI TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
BanI GGYRCC 1 cut(s) 4151
BarI GAAGNNNNNNTAC 2 cut(s) 2968, 3000
BauI CACGAG 2 cut(s) 4775, 5447
BbsI GAAGAC 5 cut(s) 732, 1567, 4119, 4911, 5311
Bbv12I GWGCWC 2 cut(s) 795, 3028
BbvCI CCTCAGC 1 cut(s) 2598
BbvI GCAGC 8 cut(s) 517, 948, 1015, 1573, 1684, 2802, 3622, 4585
BceAI ACGGC 3 cut(s) 2525, 4662, 5219
BciT130I CCWGG 9 cut(s) 929, 2052, 2265, 3021, 3689, 3714, 3809, 5403, 5491
BciVI GTATCC 3 cut(s) 136, 865, 3365
BclI TGATCA 3 cut(s) 1509, 2106, 4512
BcnI CCSGG 2 cut(s) 154, 1796
BcoDI GTCTC 4 cut(s) 832, 1884, 3133, 4084
BfmI CTRYAG 5 cut(s) 1698, 2166, 4329, 4348, 4717
BfoI RGCGCY 1 cut(s) 5103
BfuAI ACCTGC 1 cut(s) 4115
BfuI GTATCC 3 cut(s) 136, 865, 3365
BglII AGATCT 3 cut(s) 172, 1393, 3488
BisI GCNGC 9 cut(s) 531, 937, 1029, 1587, 1695, 1698, 2816, 3636, 4574
BlsI GCNGC 9 cut(s) 532, 938, 1030, 1588, 1696, 1699, 2817, 3637, 4575
BmcAI AGTACT 1 cut(s) 331
Bme18I GGWCC 2 cut(s) 2018, 3716
BmeRI GACNNNNNGTC 1 cut(s) 5295
BmeT110I CYCGRG 3 cut(s) 1049, 2066, 5017
BmgBI CACGTC 2 cut(s) 272, 730
BmgT120I GGNCC 5 cut(s) 925, 2018, 3268, 3716, 4793
BmiI GGNNCC 7 cut(s) 84, 947, 2019, 2269, 3269, 4153, 5495
BmrI ACTGGG 2 cut(s) 50, 4918
BmsI GCATC 7 cut(s) 349, 414, 487, 1459, 1830, 3266, 4560
BmtI GCTAGC 1 cut(s) 4001
BmuI ACTGGG 2 cut(s) 50, 4918
BpiI GAAGAC 5 cut(s) 732, 1567, 4119, 4911, 5311
BpmI CTGGAG 1 cut(s) 4947
Bpu10I CCTNAGC 1 cut(s) 2598
BpuEI CTTGAG 1 cut(s) 4550
BpuMI CCSGG 2 cut(s) 154, 1796
Bsa29I ATCGAT 1 cut(s) 3561
BsaAI YACGTR 4 cut(s) 3534, 3548, 4145, 5567
BsaBI GATNNNNATC 2 cut(s) 147, 5159
BsaHI GRCGYC 3 cut(s) 2980, 4548, 5316
BsaI GGTCTC 1 cut(s) 1884
BsaJI CCNNGG 7 cut(s) 152, 555, 867, 1212, 2541, 4678, 5402
BsaWI WCCGGW 2 cut(s) 2672, 3742
BsaXI ACNNNNNCTCC 4 cut(s) 2439, 2469, 5582, 5612
Bse21I CCTNAGG 2 cut(s) 941, 951
Bse3DI GCAATG 1 cut(s) 1512
Bse8I GATNNNNATC 2 cut(s) 147, 5159
BseBI CCWGG 9 cut(s) 929, 2052, 2265, 3021, 3689, 3714, 3809, 5403, 5491
BseCI ATCGAT 1 cut(s) 3561
BseDI CCNNGG 7 cut(s) 152, 555, 867, 1212, 2541, 4678, 5402
BseJI GATNNNNATC 2 cut(s) 147, 5159
BseMI GCAATG 1 cut(s) 1512
BseRI GAGGAG 2 cut(s) 3254, 5587
BseSI GKGCMC 1 cut(s) 3028
BseXI GCAGC 8 cut(s) 517, 948, 1015, 1573, 1684, 2802, 3622, 4585
BseYI CCCAGC 2 cut(s) 4974, 5206
BsgI GTGCAG 1 cut(s) 4502
Bsh1236I CGCG 2 cut(s) 2974, 3777
BshNI GGYRCC 1 cut(s) 4151
BshVI ATCGAT 1 cut(s) 3561
BsiHKAI GWGCWC 2 cut(s) 795, 3028
BsiHKCI CYCGRG 3 cut(s) 1049, 2066, 5017
BsiSI CCGG 4 cut(s) 154, 1796, 2673, 3743
BslFI GGGAC 3 cut(s) 3610, 4215, 4627
BsmAI GTCTC 4 cut(s) 832, 1884, 3133, 4084
BsmFI GGGAC 3 cut(s) 3610, 4215, 4627
BsmI GAATGC 3 cut(s) 1243, 1979, 5529
Bso31I GGTCTC 1 cut(s) 1884
BsoBI CYCGRG 3 cut(s) 1049, 2066, 5017
Bsp1286I GDGCHC 2 cut(s) 795, 3028
BspACI CCGC 5 cut(s) 1550, 1694, 2500, 3777, 5593
BspDI ATCGAT 1 cut(s) 3561
BspFNI CGCG 2 cut(s) 2974, 3777
BspHI TCATGA 3 cut(s) 97, 4165, 5286
BspLI GGNNCC 7 cut(s) 84, 947, 2019, 2269, 3269, 4153, 5495
BspMAI CTGCAG 1 cut(s) 1702
BspMI ACCTGC 1 cut(s) 4115
BspOI GCTAGC 1 cut(s) 4001
BspPI GGATC 8 cut(s) 248, 602, 635, 2215, 2344, 2436, 2524, 5243
BspQI GCTCTTC 1 cut(s) 4964
BspT107I GGYRCC 1 cut(s) 4151
BspTNI GGTCTC 1 cut(s) 1884
BsrBI CCGCTC 1 cut(s) 5595
BsrDI GCAATG 1 cut(s) 1512
BssECI CCNNGG 7 cut(s) 152, 555, 867, 1212, 2541, 4678, 5402
BssNAI GTATAC 1 cut(s) 3479
BssNI GRCGYC 3 cut(s) 2980, 4548, 5316
BssSI CACGAG 2 cut(s) 4775, 5447
BssT1I CCWWGG 1 cut(s) 867
Bst1107I GTATAC 1 cut(s) 3479
Bst2BI CACGAG 2 cut(s) 4775, 5447
Bst2UI CCWGG 9 cut(s) 929, 2052, 2265, 3021, 3689, 3714, 3809, 5403, 5491
Bst6I CTCTTC 6 cut(s) 99, 344, 1897, 2418, 2895, 4964
BstACI GRCGYC 3 cut(s) 2980, 4548, 5316
BstAPI GCANNNNNTGC 1 cut(s) 3994
BstBAI YACGTR 4 cut(s) 3534, 3548, 4145, 5567
BstC8I GCNNGC 8 cut(s) 433, 1503, 1977, 2504, 3463, 3999, 5330, 5334
BstDSI CCRYGG 2 cut(s) 2541, 4678
BstEII GGTNACC 3 cut(s) 2118, 2668, 3494
BstENI CCTNNNNNAGG 2 cut(s) 2049, 3726
BstFNI CGCG 2 cut(s) 2974, 3777
BstH2I RGCGCY 1 cut(s) 5103
BstHHI GCGC 4 cut(s) 232, 2974, 4658, 5102
BstMAI GTCTC 4 cut(s) 832, 1884, 3133, 4084
BstNI CCWGG 9 cut(s) 929, 2052, 2265, 3021, 3689, 3714, 3809, 5403, 5491
BstNSI RCATGY 8 cut(s) 230, 1654, 1664, 1792, 3046, 3465, 4357, 5332
BstPI GGTNACC 3 cut(s) 2118, 2668, 3494
BstSFI CTRYAG 5 cut(s) 1698, 2166, 4329, 4348, 4717
BstSLI GKGCMC 1 cut(s) 3028
BstSNI TACGTA 1 cut(s) 3534
BstUI CGCG 2 cut(s) 2974, 3777
BstV1I GCAGC 8 cut(s) 517, 948, 1015, 1573, 1684, 2802, 3622, 4585
BstV2I GAAGAC 5 cut(s) 732, 1567, 4119, 4911, 5311
BstX2I RGATCY 8 cut(s) 172, 640, 1393, 2220, 2349, 2428, 2529, 3488
BstXI CCANNNNNNTGG 5 cut(s) 2058, 2231, 3168, 5352, 5409
BstYI RGATCY 8 cut(s) 172, 640, 1393, 2220, 2349, 2428, 2529, 3488
BstZ17I GTATAC 1 cut(s) 3479
Bsu15I ATCGAT 1 cut(s) 3561
Bsu36I CCTNAGG 2 cut(s) 941, 951
BsuI GTATCC 3 cut(s) 136, 865, 3365
BsuTUI ATCGAT 1 cut(s) 3561
BtgI CCRYGG 2 cut(s) 2541, 4678
BtrI CACGTC 2 cut(s) 272, 730
BtsI GCAGTG 1 cut(s) 1695
BveI ACCTGC 1 cut(s) 4115
Cac8I GCNNGC 8 cut(s) 433, 1503, 1977, 2504, 3463, 3999, 5330, 5334
CciI TCATGA 3 cut(s) 97, 4165, 5286
CfoI GCGC 4 cut(s) 232, 2974, 4658, 5102
Cfr13I GGNCC 5 cut(s) 925, 2018, 3268, 3716, 4793
ClaI ATCGAT 1 cut(s) 3561
CseI GACGC 5 cut(s) 1561, 2969, 3227, 3529, 4556
CsiI ACCWGGT 1 cut(s) 3687
CspCI CAANNNNNGTGG 2 cut(s) 2523, 2558
DraI TTTAAA 1 cut(s) 5107
DraIII CACNNNGTG 1 cut(s) 3929
DriI GACNNNNNGTC 1 cut(s) 5295
EaeI YGGCCR 5 cut(s) 2504, 2520, 2785, 4500, 4675
Eam1104I CTCTTC 6 cut(s) 99, 344, 1897, 2418, 2895, 4964
Eam1105I GACNNNNNGTC 1 cut(s) 5295
EarI CTCTTC 6 cut(s) 99, 344, 1897, 2418, 2895, 4964
Eco105I TACGTA 1 cut(s) 3534
Eco130I CCWWGG 1 cut(s) 867
Eco147I AGGCCT 1 cut(s) 3332
Eco31I GGTCTC 1 cut(s) 1884
Eco32I GATATC 1 cut(s) 3887
Eco47I GGWCC 2 cut(s) 2018, 3716
Eco57I CTGAAG 1 cut(s) 3311
Eco81I CCTNAGG 2 cut(s) 941, 951
Eco88I CYCGRG 3 cut(s) 1049, 2066, 5017
Eco91I GGTNACC 3 cut(s) 2118, 2668, 3494
EcoNI CCTNNNNNAGG 2 cut(s) 2049, 3726
EcoO109I RGGNCCY 1 cut(s) 3716
EcoO65I GGTNACC 3 cut(s) 2118, 2668, 3494
EcoRII CCWGG 9 cut(s) 927, 2050, 2263, 3019, 3687, 3712, 3807, 5401, 5489
EcoRV GATATC 1 cut(s) 3887
EcoT14I CCWWGG 1 cut(s) 867
EcoT22I ATGCAT 1 cut(s) 1914
ErhI CCWWGG 1 cut(s) 867
FaqI GGGAC 3 cut(s) 3610, 4215, 4627
FauNDI CATATG 2 cut(s) 1077, 3678
FbaI TGATCA 3 cut(s) 1509, 2106, 4512
FblI GTMKAC 3 cut(s) 3478, 5439, 5554
Fnu4HI GCNGC 9 cut(s) 531, 937, 1029, 1587, 1695, 1698, 2816, 3636, 4574
Fsp4HI GCNGC 9 cut(s) 531, 937, 1029, 1587, 1695, 1698, 2816, 3636, 4574
GlaI GCGC 4 cut(s) 231, 2973, 4657, 5101
GluI GCNGC 9 cut(s) 531, 937, 1029, 1587, 1695, 1698, 2816, 3636, 4574
GsaI CCCAGC 2 cut(s) 4978, 5210
GsuI CTGGAG 1 cut(s) 4947
HaeII RGCGCY 1 cut(s) 5103
HapII CCGG 4 cut(s) 154, 1796, 2673, 3743
HgaI GACGC 5 cut(s) 1561, 2969, 3227, 3529, 4556
HhaI GCGC 4 cut(s) 232, 2974, 4658, 5102
Hin1I GRCGYC 3 cut(s) 2980, 4548, 5316
Hin6I GCGC 4 cut(s) 230, 2972, 4656, 5100
HinP1I GCGC 4 cut(s) 230, 2972, 4656, 5100
HincII GTYRAC 3 cut(s) 1656, 1965, 4359
HindII GTYRAC 3 cut(s) 1656, 1965, 4359
HindIII AAGCTT 5 cut(s) 1608, 1973, 3387, 3828, 4086
HpaI GTTAAC 2 cut(s) 1965, 4359
HpaII CCGG 4 cut(s) 154, 1796, 2673, 3743
Hpy99I CGWCG 3 cut(s) 1830, 3221, 5205
HpyAV CCTTC 9 cut(s) 128, 1229, 2970, 3343, 3728, 4006, 4142, 4741, 4987
Hsp92I GRCGYC 3 cut(s) 2980, 4548, 5316
HspAI GCGC 4 cut(s) 230, 2972, 4656, 5100
Ksp22I TGATCA 3 cut(s) 1509, 2106, 4512
KspAI GTTAAC 2 cut(s) 1965, 4359
LguI GCTCTTC 1 cut(s) 4964
LmnI GCTCC 9 cut(s) 1797, 1996, 2198, 2267, 3322, 3643, 3964, 4861, 5600
Lsp1109I GCAGC 8 cut(s) 517, 948, 1015, 1573, 1684, 2802, 3622, 4585
LweI GCATC 7 cut(s) 349, 414, 487, 1459, 1830, 3266, 4560
MabI ACCWGGT 1 cut(s) 3687
MbiI CCGCTC 1 cut(s) 5595
MfeI CAATTG 1 cut(s) 2605
MflI RGATCY 8 cut(s) 172, 640, 1393, 2220, 2349, 2428, 2529, 3488
MhlI GDGCHC 2 cut(s) 795, 3028
MlsI TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
MluNI TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
MlyI GAGTC 6 cut(s) 996, 1183, 2948, 3135, 4168, 4197
MmeI TCCRAC 8 cut(s) 834, 1083, 1393, 1849, 2045, 2164, 2985, 5245
Mox20I TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
Mph1103I ATGCAT 1 cut(s) 1914
MroXI GAANNNNTTC 3 cut(s) 1243, 3931, 5007
MscI TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
MslI CAYNNNNRTG 6 cut(s) 225, 1917, 3014, 3452, 4038, 4508
Msp20I TGGCCA 4 cut(s) 2506, 2522, 2787, 4502
MspA1I CMGCKG 2 cut(s) 1586, 2502
MspI CCGG 4 cut(s) 154, 1796, 2673, 3743
MunI CAATTG 1 cut(s) 2605
Mva1269I GAATGC 3 cut(s) 1243, 1979, 5529
MvaI CCWGG 9 cut(s) 929, 2052, 2265, 3021, 3689, 3714, 3809, 5403, 5491
MvnI CGCG 2 cut(s) 2974, 3777
NciI CCSGG 2 cut(s) 154, 1796
NdeI CATATG 2 cut(s) 1077, 3678
NheI GCTAGC 1 cut(s) 3997
NlaIV GGNNCC 7 cut(s) 84, 947, 2019, 2269, 3269, 4153, 5495
NmeAIII GCCGAG 2 cut(s) 580, 1237
NsiI ATGCAT 1 cut(s) 1914
NspI RCATGY 8 cut(s) 230, 1654, 1664, 1792, 3046, 3465, 4357, 5332
OliI CACNNNNGTG 1 cut(s) 3452
PaeI GCATGC 2 cut(s) 3465, 5332
PagI TCATGA 3 cut(s) 97, 4165, 5286
PceI AGGCCT 1 cut(s) 3332
PciI ACATGT 1 cut(s) 1660
PciSI GCTCTTC 1 cut(s) 4964
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 1272
PctI GAATGC 3 cut(s) 1243, 1979, 5529
PdmI GAANNNNTTC 3 cut(s) 1243, 3931, 5007
PflFI GACNNNGTC 1 cut(s) 3689
PfoI TCCNGGA 1 cut(s) 1794
PkrI GCNGC 9 cut(s) 532, 938, 1030, 1588, 1696, 1699, 2817, 3637, 4575
PleI GAGTC 6 cut(s) 996, 1183, 2947, 3134, 4167, 4197
PpsI GAGTC 6 cut(s) 996, 1183, 2947, 3134, 4167, 4197
Ppu21I YACGTR 4 cut(s) 3534, 3548, 4145, 5567
PpuMI RGGWCCY 1 cut(s) 3716
PscI ACATGT 1 cut(s) 1660
PshBI ATTAAT 4 cut(s) 2409, 3362, 4101, 5339
Psp5II RGGWCCY 1 cut(s) 3716
Psp6I CCWGG 9 cut(s) 927, 2050, 2263, 3019, 3687, 3712, 3807, 5401, 5489
PspEI GGTNACC 3 cut(s) 2118, 2668, 3494
PspFI CCCAGC 2 cut(s) 4974, 5206
PspGI CCWGG 9 cut(s) 927, 2050, 2263, 3019, 3687, 3712, 3807, 5401, 5489
PspN4I GGNNCC 7 cut(s) 84, 947, 2019, 2269, 3269, 4153, 5495
PspPI GGNCC 5 cut(s) 925, 2018, 3268, 3716, 4793
PspPPI RGGWCCY 1 cut(s) 3716
PsrI GAACNNNNNNTAC 4 cut(s) 1931, 1963, 3388, 3420
PstI CTGCAG 1 cut(s) 1702
PsuI RGATCY 8 cut(s) 172, 640, 1393, 2220, 2349, 2428, 2529, 3488
PsyI GACNNNGTC 1 cut(s) 3689
PvuII CAGCTG 1 cut(s) 1586
RseI CAYNNNNRTG 6 cut(s) 225, 1917, 3014, 3452, 4038, 4508
SapI GCTCTTC 1 cut(s) 4964
SatI GCNGC 9 cut(s) 531, 937, 1029, 1587, 1695, 1698, 2816, 3636, 4574
Sau96I GGNCC 5 cut(s) 925, 2018, 3268, 3716, 4793
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 6 cut(s) 996, 1183, 2948, 3135, 4168, 4197
SduI GDGCHC 2 cut(s) 795, 3028
SexAI ACCWGGT 1 cut(s) 3687
SfaNI GCATC 7 cut(s) 349, 414, 487, 1459, 1830, 3266, 4560
SfcI CTRYAG 5 cut(s) 1698, 2166, 4329, 4348, 4717
SinI GGWCC 2 cut(s) 2018, 3716
SmiMI CAYNNNNRTG 6 cut(s) 225, 1917, 3014, 3452, 4038, 4508
SmlI CTYRAG 1 cut(s) 4529
SmoI CTYRAG 1 cut(s) 4529
SnaBI TACGTA 1 cut(s) 3534
SphI GCATGC 2 cut(s) 3465, 5332
SseBI AGGCCT 1 cut(s) 3332
SsiI CCGC 5 cut(s) 1550, 1694, 2500, 3777, 5593
SspI AATATT 4 cut(s) 595, 2868, 3156, 5274
StuI AGGCCT 1 cut(s) 3332
StyI CCWWGG 1 cut(s) 867
TaqI TCGA 8 cut(s) 606, 862, 2206, 2419, 3216, 3561, 4823, 4902
TatI WGTACW 2 cut(s) 329, 1938
TauI GCSGC 1 cut(s) 1697
TseI GCWGC 8 cut(s) 530, 936, 1028, 1586, 1697, 2815, 3635, 4573
TspGWI ACGGA 4 cut(s) 4257, 4295, 4579, 5094
Tth111I GACNNNGTC 1 cut(s) 3689
VneI GTGCAC 1 cut(s) 3024
VpaK11BI GGWCC 2 cut(s) 2018, 3716
VspI ATTAAT 4 cut(s) 2409, 3362, 4101, 5339
XagI CCTNNNNNAGG 2 cut(s) 2049, 3726
XapI RAATTY 8 cut(s) 847, 2758, 3443, 3607, 3857, 4212, 4619, 4703
XbaI TCTAGA 1 cut(s) 2246
XceI RCATGY 8 cut(s) 230, 1654, 1664, 1792, 3046, 3465, 4357, 5332
XcmI CCANNNNNNNNNTGG 2 cut(s) 3103, 4971
XmiI GTMKAC 3 cut(s) 3478, 5439, 5554
XmnI GAANNNNTTC 3 cut(s) 1243, 3931, 5007
ZraI GACGTC 1 cut(s) 5317
ZrmI AGTACT 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 1914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.