MD07G1001800.v1.1

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002059
Physical Location & Seq
Forward (+)
235867 .. 251143
15277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1001800.v1.1.491

Sequence Viewer

Length: 4236 bp
ATGGCATATCGAAGAGGAGGGTTTGAAGGGCAGCCGCAGAGGCGGATTCTGCGCACCCAGACTGCTGGGAGTTTTGGAGAGCCCATGCTGGATAGTGAGGTGGTGCCTTCTTCCCTCGTTGACATTGCTCCTATTCTCCGTGTTGCAAACGAAGTTGAGTCCAGGAATCCAAGAGTTGCCTATCTATGTCGGTTCTATGCCTTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGACGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAGCGTCTAGAAAGGGAAAATGAAACAACACTGGCAGGAAGGGCAAAGAGTGATGCTCGGGAAATGCAGAGTTTTTATAGAGATTACTACAAAAAATACATTCAAGCTTTGCAGAATGCTGCTGATAAAGCAGATCGGGCACAACTTACAAAAGCATACCAAACTGCTGCTGTTTTATTTGAGGTTTTGAAGGCTGTCAATCAGACGGAGGCTGTTGAAGTAGCTGAGGAGATTTTGGAAGCTCATACCAAGGTTGAAGAAAAACAGCAGATATATGTACCTTACAATATTCTTCCTCTTGATCCTGATAGTCAAAATCAGGCGATCATGAGATATCCCGAGATTCATGCTACTGTTTCTGCACTCCGTAACACCAGGGGTCTACCATGGCCGAAGGCCCACAAAAAGAAAGTAGATGAAGACATTCTAGACTGGCTTCAGGCCATGTTTGGCTTTCAGAAAGATAATGTGGCAAATCAAAGGGAACACTTGATTCTGCTGGTTGCTAATGTGCACATACGGCAATTACCGAATCCTGATCAACAACCCAAGTTGGATGATCGCGCTTTAACGGATGTGATGAAGAAGCTTTTCAAAAATTACAAAAAGTGGTGTAAATACCTGGATCGGAAAAGTAGCCTTTGGCTGCCAACTATTCAGCAGGAAGTACAGCAACGAAAATTGCTATACATGGGTTTATATCTTCTTATATGGGGAGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGCTATATCTATCATCATATGGCATTTGAATTGTATGGAATGCTAGCTGGGAGTGTTAGTCCATTGACAGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGATGAGGCCTTTTTGTCGAAAGTAGTCACTCCAATATATGATACAATAGCAAAGGAGGCTAAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGACGACTTGAATGAATATTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCGGATGCTGATTTCTTTTGCCAGCCTATCGACGATATTCAAGGTGATAGAGATGAGAGAAAGAAACCATATACTGGGGACCGATGGATTGGCAAAGTAAACTTTGTCGAAATACGCTCATTTTTGCACATTTTCAGAAGTTTTGATAGGATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATTGTTGCTTGGAATGGGTCGGGTGACTTAGGTTCCATGTTTGAGGGTGATGTCTTTAAGAAAGTGCTGAGTATCTTCATAACTGCTGCGATATTGAAGCTTGCACAAGCTGTTCTTGATTTAATTTTGAGCTGGAAAGCTAGGCAAAGCATGTCAGTCTTTGTCAAGTTAAGATATGTCTTGAAGGCTGTTTCAGCTGCAGCGTGGGTCATTGTTTTACCAGTGACTTACGCATTTAGTTGGAAAGATCCTACTGGATTTGCACGGACCATCAAAACTTGGTTTGGCAATGGTCCAAGTTCAAATTCTTTGTTCATTTTGGCTGTTGTCATCTACTTATCTCCAAACATGCTCTCTGGATTGTTGTTTATGTTCCCATTCATTCGCCGGTTTCTTGAAAGGTCAAACTTAAAGCTTGTGATGCTCATGATGTGGTGGTCTCAGACCCGGCTTTATGTTGGAAGGGGAATGCATGAAAGCTCAATTTCTCTTTTCAAGTATACCCTTTTTTGGGTTCTCCTGCTTGTATCGAAATTAGCCTTCAGTTACTACGTAGAGATTAGGCCTCTTGTAAAACCAACTAAAGATATAATGAGGGCTCGCGTAAAGACCTACCAATGGCATGAGTTCTTCCCTCAAGCCAAGAATAACATTGGTGTGGTGATTTCGCTATGGACTCCCATTGTTCTTGTCTACTTTATGGATACTCAAATTTGGTATGCCATATTTTCCACAATATTCGGAGGTATATACGGTGCATTTCGCCGTCTTGGAGAGATTCGAACGCTGGGAATGTTGCGGTCTCGATTTGAATCACTGCCAGGTGCTTTTAATAGTTTTTTGATTCCAGTGGAAAGGAATGAGCAGACTAAAAAGAAGGGAATACTAAAAGCCACTTTCTCTCGCAAGTTTGATAAGACCCCATCTAGTAAAGAAAAGGAAGCAGCAAAGTTTGCACAGATGTGGAACGAAATAATTAGCAGTTTCAGAGAAGAGGATCTGATTAGTGATAGGGAAATGAACTTGTTGCTCGTTCCATATGGGGCTGACCCAGATTTAGTAGACCTAATCCAGTGGCCTCCTTTTCTGCTTGCTAGCAAGATCCCGATAGCACTGGACATGGCAAAAGACAGCAAAGATAAAGACCGTGAGCTGAAAAAGAGAATGAGTACTGACAATTATATGCGCTGTGCTATTCACGAATGCTATCTCTCGTTTAGAAGTATTATTAACTTTTTGGTCCTTGGAGATCGTGAAAAGAAGGATATAAATGAAATCTTCGCCATAGTTGACGATCATATTGAAAAGGGAAACCTGACAACAGAGTTCAATATGAGTGCTCTTCCAAGTCTCCATGAACAATTTGTCAAGTTAATTGAATATCTGATGGAAAACAAAAAGGAGGACAAGGACCAGGTTGTAATTGTCTTGCTCAACATGTTAGAGGTTGTGACAAGAGACATAATGGAGGATGAAAGTCCCAACTTGCTAGAGTCGAGTGATGGATTACATGGAAAAGATGAGGGAATGACACCCCTTGATCAACGGGATACATATTTTGGTGCACTGAGGTTCCCAGTCCCAGTGACTGCAAAAACAGGAGCTTGGAAAGAAAAGATCAGAAGGCTACATCTATTACTTACAGAGAAAGAGTCTGCTATGGATGTCCCATCCAACTTGGAAGCAAGAAGGCGCATTTCTTTCTTCTCCAACTCGTTGTTTATGGACATGCCTCCTGCACCCAAAGTTCGAAATATGCTTTCCTTCTCGGTTTTGACACCATACTATTCAGAAGACGTTCTTTTCTCCAAAAAACATCTGGAATGGCAGAACGAAGATGGTGTCTCTATCCTTTTCTACTTGCAGAAGATCTTTCCAGATGAATGGACTAACTTTCTTGAGCGGGTCAAGTGTGAAAATGAAGAAGAACTCACAGAAAACGATGAATTGGAAGAGAAACTCCGTCTATGGGCATCCTATCGAGGCCAAACATTGACCAAAACTGTACGGGGTATGATGTATTACCGCAAAGCTTTGGAACTTCAGGCCTTCCTTGATATGGCAAAAGATGAAGAGCTAATGGAAGGCTATAAGGCTGCTGAATCAACTATTGAGGAACACTCAAAAGCAGAAAGGTCACTTTTGGCACAATGTCAGGCAGTGGTTGATATGAAATTCTCATATGTTGTGTCATGCCAACAATATGGAATTCACAAGCGATCTGGAGATGCTCGTTCCAAGGACATTTTGAAACTTATGGCAACTTATCCATCTCTTCGAGTAGCTTACATTGATGAGGTAGAGAAAACTAGTGAAGATAAATCCAAAAAGAATGTTTGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACTAAGTCAGTTGATTCTTCTGATCCAGTTCAAAGGTTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCTGCAATATTGGGAGGGGGAAAGCCAGAAAATCAAAACCACGCCATCATTTTCACACGTGGGGAAGGCTTGCAAACCATAGATATGAACCAGGATAATTACTTAGAAGAAGCATTCAAAATGAGGAACCTACTGCAAGAATTTCTTGTGAAGCATGATGGTGTTAGATTTCCGACAATACTTGGACTTAGGGAGCATATTTTCACTGGCAGTGTTTCATCTCTTGCATGGTTTATGTCAAATCAAGAGACTTGTTTTGTAACTATTGGGCAGAGATTGCTTGCTAACCCTCTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1412

Amino Acids

163.2

Weight (kDa)

8.72

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 169 2.3e-18 Vta1 like
FKS1_dom1 PF14288 318 - 430 2.7e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 855 - 1043 2.9e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1143 1.8e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1143 - 1408 1.3e-76 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 3946
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 2 cut(s) 1118, 1370
AccBSI CCGCTC 1 cut(s) 3388
AccI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
AccII CGCG 2 cut(s) 837, 2088
AciI CCGC 7 cut(s) 35, 43, 1298, 2284, 3388, 3511, 3854
AclWI GGATC 7 cut(s) 218, 569, 906, 1727, 2490, 2581, 3881
AcoI YGGCCR 1 cut(s) 662
AcsI RAATTY 6 cut(s) 1000, 1789, 2196, 3659, 3693, 4076
AcuI CTGAAG 4 cut(s) 257, 695, 2011, 3512
AcvI CACGTG 1 cut(s) 3995
AfaI GTAC 4 cut(s) 552, 942, 2656, 3492
AfiI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
AflIII ACRYGT 2 cut(s) 2922, 3992
AhlI ACTAGT 1 cut(s) 3794
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 8 cut(s) 161, 647, 894, 2305, 2898, 3906, 3931, 4026
AjuI GAANNNNNNNTTGG 2 cut(s) 1774, 1806
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 4 cut(s) 789, 1104, 2827, 3052
Alw26I GTCTC 6 cut(s) 1929, 2292, 2840, 2937, 3334, 4178
Alw44I GTGCAC 2 cut(s) 785, 3048
AlwI GGATC 7 cut(s) 218, 569, 906, 1727, 2490, 2581, 3881
AlwNI CAGNNNCTG 1 cut(s) 3074
Ama87I CYCGRG 2 cut(s) 330, 611
AoxI GGCC 9 cut(s) 662, 669, 714, 1134, 1286, 2048, 2561, 3469, 3531
ApaLI GTGCAC 2 cut(s) 785, 3048
ApoI RAATTY 6 cut(s) 1000, 1789, 2196, 3659, 3693, 4076
ArsI GACNNNNNNTTYG 4 cut(s) 231, 263, 3312, 3344
Asp700I GAANNNNTTC 3 cut(s) 696, 863, 3282
AspLEI GCGC 4 cut(s) 54, 839, 2673, 3180
AspS9I GGNCC 8 cut(s) 670, 1375, 1752, 1778, 2725, 2896, 3904, 3935
AsuC2I CCSGG 1 cut(s) 1933
AsuHPI GGTGA 6 cut(s) 1109, 1136, 1352, 1520, 1544, 2158
AsuII TTCGAA 2 cut(s) 2266, 3235
AsuNHI GCTAGC 2 cut(s) 1069, 2579
AvaI CYCGRG 2 cut(s) 330, 611
AvaII GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
BaeGI GKGCMC 3 cut(s) 415, 789, 3052
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 2 cut(s) 84, 2086
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 2 cut(s) 699, 3285
Bbv12I GWGCWC 4 cut(s) 789, 1104, 2827, 3052
BbvCI CCTCAGC 1 cut(s) 498
BceAI ACGGC 2 cut(s) 809, 2235
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 8 cut(s) 163, 649, 896, 2307, 2900, 3908, 3933, 4028
BciVI GTATCC 2 cut(s) 2182, 3028
BclI TGATCA 3 cut(s) 811, 1482, 3025
BcnI CCSGG 1 cut(s) 1933
BcoDI GTCTC 6 cut(s) 1929, 2292, 2840, 2937, 3334, 4178
BcuI ACTAGT 1 cut(s) 3794
BfmI CTRYAG 1 cut(s) 1683
BfuAI ACCTGC 1 cut(s) 3946
BfuI GTATCC 2 cut(s) 2182, 3028
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 1 cut(s) 3354
BmcAI AGTACT 1 cut(s) 2656
Bme1390I CCNGG 9 cut(s) 163, 649, 896, 1933, 2307, 2900, 3908, 3933, 4028
Bme18I GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
BmeT110I CYCGRG 2 cut(s) 330, 611
BmgBI CACGTC 1 cut(s) 242
BmgT120I GGNCC 8 cut(s) 670, 1375, 1752, 1778, 2725, 2896, 3904, 3935
BmiI GGNNCC 6 cut(s) 105, 1376, 1519, 3059, 3858, 4064
BmrFI CCNGG 9 cut(s) 163, 649, 896, 1933, 2307, 2900, 3908, 3933, 4028
BmrI ACTGGG 3 cut(s) 1380, 3056, 3062
BmsI GCATC 5 cut(s) 316, 1291, 1896, 3467, 3703
BmtI GCTAGC 2 cut(s) 1073, 2583
BmuI ACTGGG 3 cut(s) 1380, 3056, 3062
BpiI GAAGAC 2 cut(s) 699, 3285
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 3590, 3622
BpmI CTGGAG 1 cut(s) 3729
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 2266, 3235
BpuEI CTTGAG 2 cut(s) 2106, 3404
BpuMI CCSGG 1 cut(s) 1933
BsaAI YACGTR 2 cut(s) 2038, 3995
BsaBI GATNNNNATC 2 cut(s) 2296, 3915
BsaI GGTCTC 2 cut(s) 1929, 2292
BsaJI CCNNGG 5 cut(s) 522, 648, 659, 2728, 3723
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
Bse118I RCCGGY 1 cut(s) 1872
Bse3DI GCAATG 3 cut(s) 123, 1485, 1780
Bse8I GATNNNNATC 2 cut(s) 2296, 3915
BseBI CCWGG 8 cut(s) 163, 649, 896, 2307, 2900, 3908, 3933, 4028
BseDI CCNNGG 5 cut(s) 522, 648, 659, 2728, 3723
BseGI GGATG 8 cut(s) 835, 853, 1306, 1452, 2962, 3154, 3155, 3458
BseJI GATNNNNATC 2 cut(s) 2296, 3915
BseLI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
BseMI GCAATG 3 cut(s) 123, 1485, 1780
BseMII CTCAG 5 cut(s) 489, 1544, 1940, 3044, 4220
BseRI GAGGAG 3 cut(s) 30, 515, 3849
BseSI GKGCMC 3 cut(s) 415, 789, 3052
BseYI CCCAGC 3 cut(s) 65, 1073, 2272
BsgI GTGCAG 3 cut(s) 249, 618, 3207
Bsh1236I CGCG 2 cut(s) 837, 2088
BshFI GGCC 9 cut(s) 664, 671, 716, 1136, 1288, 2050, 2563, 3471, 3533
BshNI GGYRCC 1 cut(s) 103
BsiHKAI GWGCWC 4 cut(s) 789, 1104, 2827, 3052
BsiHKCI CYCGRG 2 cut(s) 330, 611
BsiSI CCGG 2 cut(s) 1873, 1933
BslFI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
BslI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
BsmAI GTCTC 6 cut(s) 1929, 2292, 2840, 2937, 3334, 4178
BsmFI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
BsmI GAATGC 7 cut(s) 394, 1025, 1071, 1216, 1959, 2693, 4049
BsnI GGCC 9 cut(s) 664, 671, 716, 1136, 1288, 2050, 2563, 3471, 3533
Bso31I GGTCTC 2 cut(s) 1929, 2292
BsoBI CYCGRG 2 cut(s) 330, 611
Bsp119I TTCGAA 2 cut(s) 2266, 3235
Bsp1286I GDGCHC 7 cut(s) 84, 415, 789, 1104, 2086, 2827, 3052
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 7 cut(s) 35, 43, 1298, 2284, 3388, 3511, 3854
BspANI GGCC 9 cut(s) 664, 671, 716, 1136, 1288, 2050, 2563, 3471, 3533
BspCNI CTCAG 5 cut(s) 490, 1545, 1939, 3045, 4221
BspFNI CGCG 2 cut(s) 837, 2088
BspHI TCATGA 2 cut(s) 600, 1911
BspLI GGNNCC 6 cut(s) 105, 1376, 1519, 3059, 3858, 4064
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 1 cut(s) 3946
BspOI GCTAGC 2 cut(s) 1073, 2583
BspPI GGATC 7 cut(s) 218, 569, 906, 1727, 2490, 2581, 3881
BspQI GCTCTTC 2 cut(s) 2832, 3552
BspT104I TTCGAA 2 cut(s) 2266, 3235
BspT107I GGYRCC 1 cut(s) 103
BspTNI GGTCTC 2 cut(s) 1929, 2292
BsrBI CCGCTC 1 cut(s) 3388
BsrDI GCAATG 3 cut(s) 123, 1485, 1780
BsrFI RCCGGY 1 cut(s) 1872
BssAI RCCGGY 1 cut(s) 1872
BssECI CCNNGG 5 cut(s) 522, 648, 659, 2728, 3723
BssNAI GTATAC 1 cut(s) 1986
BssT1I CCWWGG 4 cut(s) 522, 659, 2728, 3723
Bst1107I GTATAC 1 cut(s) 1986
Bst2UI CCWGG 8 cut(s) 163, 649, 896, 2307, 2900, 3908, 3933, 4028
Bst4CI ACNGT 4 cut(s) 628, 2240, 2633, 3490
Bst6I CTCTTC 6 cut(s) 7, 2472, 2832, 3432, 3552, 3765
BstAPI GCANNNNNTGC 2 cut(s) 2438, 4213
BstBAI YACGTR 2 cut(s) 2038, 3995
BstBI TTCGAA 2 cut(s) 2266, 3235
BstDEI CTNAG 9 cut(s) 498, 1513, 1553, 1926, 3053, 3867, 4039, 4124, 4229
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 8 cut(s) 835, 853, 1306, 1452, 2962, 3154, 3155, 3458
BstFNI CGCG 2 cut(s) 837, 2088
BstHHI GCGC 4 cut(s) 54, 839, 2673, 3180
BstMAI GTCTC 6 cut(s) 1929, 2292, 2840, 2937, 3334, 4178
BstNI CCWGG 8 cut(s) 163, 649, 896, 2307, 2900, 3908, 3933, 4028
BstNSI RCATGY 4 cut(s) 1639, 1837, 2926, 3217
BstSCI CCNGG 9 cut(s) 161, 647, 894, 1931, 2305, 2898, 3906, 3931, 4026
BstSFI CTRYAG 1 cut(s) 1683
BstSLI GKGCMC 3 cut(s) 415, 789, 3052
BstSNI TACGTA 1 cut(s) 2038
BstUI CGCG 2 cut(s) 837, 2088
BstV2I GAAGAC 2 cut(s) 699, 3285
BstX2I RGATCY 5 cut(s) 223, 1732, 2482, 2586, 3354
BstXI CCANNNNNNTGG 3 cut(s) 65, 3369, 3689
BstYI RGATCY 5 cut(s) 223, 1732, 2482, 2586, 3354
BstZ17I GTATAC 1 cut(s) 1986
BsuI GTATCC 2 cut(s) 2182, 3028
BsuRI GGCC 9 cut(s) 664, 671, 716, 1136, 1288, 2050, 2563, 3471, 3533
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 8 cut(s) 835, 853, 1306, 1452, 2962, 3154, 3155, 3458
BtsI GCAGTG 3 cut(s) 2300, 3651, 4153
BveI ACCTGC 1 cut(s) 3946
CaiI CAGNNNCTG 1 cut(s) 3074
CciI TCATGA 2 cut(s) 600, 1911
CfoI GCGC 4 cut(s) 54, 839, 2673, 3180
Cfr10I RCCGGY 1 cut(s) 1872
Cfr13I GGNCC 8 cut(s) 670, 1375, 1752, 1778, 2725, 2896, 3904, 3935
CseI GACGC 1 cut(s) 266
CsiI ACCWGGT 1 cut(s) 2898
Csp6I GTAC 4 cut(s) 551, 941, 2655, 3491
CviQI GTAC 4 cut(s) 551, 941, 2655, 3491
DdeI CTNAG 9 cut(s) 498, 1513, 1553, 1926, 3053, 3867, 4039, 4124, 4229
DraI TTTAAA 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 662
Eam1104I CTCTTC 6 cut(s) 7, 2472, 2832, 3432, 3552, 3765
EarI CTCTTC 6 cut(s) 7, 2472, 2832, 3432, 3552, 3765
EciI GGCGGA 1 cut(s) 58
Eco105I TACGTA 1 cut(s) 2038
Eco130I CCWWGG 4 cut(s) 522, 659, 2728, 3723
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3533
Eco24I GRGCYC 2 cut(s) 84, 2086
Eco31I GGTCTC 2 cut(s) 1929, 2292
Eco32I GATATC 1 cut(s) 608
Eco47I GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
Eco57I CTGAAG 4 cut(s) 257, 695, 2011, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco88I CYCGRG 2 cut(s) 330, 611
EcoRI GAATTC 1 cut(s) 3693
EcoRII CCWGG 8 cut(s) 161, 647, 894, 2305, 2898, 3906, 3931, 4026
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 4 cut(s) 522, 659, 2728, 3723
EcoT22I ATGCAT 1 cut(s) 1959
EcoT38I GRGCYC 2 cut(s) 84, 2086
ErhI CCWWGG 4 cut(s) 522, 659, 2728, 3723
FalI AAGNNNNNCTT 6 cut(s) 1584, 1616, 3270, 3302, 4065, 4097
FaqI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
FauI CCCGC 1 cut(s) 3381
FauNDI CATATG 3 cut(s) 1044, 2524, 3667
FbaI TGATCA 3 cut(s) 811, 1482, 3025
FblI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
FokI GGATG 8 cut(s) 842, 860, 1313, 1459, 2969, 3142, 3161, 3445
FriOI GRGCYC 2 cut(s) 84, 2086
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 4 cut(s) 53, 838, 2672, 3179
GsaI CCCAGC 3 cut(s) 69, 1077, 2276
GsuI CTGGAG 1 cut(s) 3729
HaeIII GGCC 9 cut(s) 664, 671, 716, 1136, 1288, 2050, 2563, 3471, 3533
HapII CCGG 2 cut(s) 1873, 1933
HgaI GACGC 1 cut(s) 266
HhaI GCGC 4 cut(s) 54, 839, 2673, 3180
Hin6I GCGC 4 cut(s) 52, 837, 2671, 3178
HinP1I GCGC 4 cut(s) 52, 837, 2671, 3178
HincII GTYRAC 2 cut(s) 121, 2776
HindII GTYRAC 2 cut(s) 121, 2776
HindIII AAGCTT 6 cut(s) 378, 860, 1583, 1898, 3516, 3926
HpaII CCGG 2 cut(s) 1873, 1933
HphI GGTGA 6 cut(s) 1109, 1136, 1352, 1520, 1544, 2158
Hpy99I CGWCG 1 cut(s) 1331
HpyCH4III ACNGT 4 cut(s) 628, 2240, 2633, 3490
HpyCH4IV ACGT 4 cut(s) 241, 2037, 3282, 3994
HpyF3I CTNAG 9 cut(s) 498, 1513, 1553, 1926, 3053, 3867, 4039, 4124, 4229
HpySE526I ACGT 4 cut(s) 241, 2037, 3282, 3994
HspAI GCGC 4 cut(s) 52, 837, 2671, 3178
Ksp22I TGATCA 3 cut(s) 811, 1482, 3025
LguI GCTCTTC 2 cut(s) 2832, 3552
LmnI GCTCC 6 cut(s) 133, 1194, 1452, 3086, 3862, 4129
LweI GCATC 5 cut(s) 316, 1291, 1896, 3467, 3703
MabI ACCWGGT 1 cut(s) 2898
MaeII ACGT 4 cut(s) 241, 2037, 3282, 3994
MaeIII GTNAC 9 cut(s) 641, 1153, 1508, 1708, 2030, 2935, 3070, 3621, 4195
MbiI CCGCTC 1 cut(s) 3388
MflI RGATCY 5 cut(s) 223, 1732, 2482, 2586, 3354
MhlI GDGCHC 7 cut(s) 84, 415, 789, 1104, 2086, 2827, 3052
MlyI GAGTC 4 cut(s) 167, 2155, 2987, 3146
MmeI TCCRAC 8 cut(s) 807, 1706, 1924, 3183, 3219, 3882, 3887, 4133
Mph1103I ATGCAT 1 cut(s) 1959
MroXI GAANNNNTTC 3 cut(s) 696, 863, 3282
MslI CAYNNNNRTG 4 cut(s) 2141, 2446, 4019, 4095
MspA1I CMGCKG 1 cut(s) 1682
MspI CCGG 2 cut(s) 1873, 1933
MspR9I CCNGG 9 cut(s) 163, 649, 896, 1933, 2307, 2900, 3908, 3933, 4028
Mva1269I GAATGC 7 cut(s) 394, 1025, 1071, 1216, 1959, 2693, 4049
MvaI CCWGG 8 cut(s) 163, 649, 896, 2307, 2900, 3908, 3933, 4028
MvnI CGCG 2 cut(s) 837, 2088
NciI CCSGG 1 cut(s) 1933
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 3 cut(s) 1044, 2524, 3667
NheI GCTAGC 2 cut(s) 1069, 2579
NlaIV GGNNCC 6 cut(s) 105, 1376, 1519, 3059, 3858, 4064
NmuCI GTSAC 6 cut(s) 1153, 1508, 1708, 2935, 3070, 3621
NsbI TGCGCA 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 1959
NspI RCATGY 4 cut(s) 1639, 1837, 2926, 3217
NspV TTCGAA 2 cut(s) 2266, 3235
OliI CACNNNNGTG 1 cut(s) 2446
PagI TCATGA 2 cut(s) 600, 1911
PceI AGGCCT 3 cut(s) 1136, 2050, 3533
PciI ACATGT 1 cut(s) 2922
PciSI GCTCTTC 2 cut(s) 2832, 3552
PctI GAATGC 7 cut(s) 394, 1025, 1071, 1216, 1959, 2693, 4049
PdmI GAANNNNTTC 3 cut(s) 696, 863, 3282
PflMI CCANNNNNTGG 2 cut(s) 1118, 1370
PfoI TCCNGGA 2 cut(s) 161, 3931
PleI GAGTC 4 cut(s) 166, 2155, 2986, 3145
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 4 cut(s) 166, 2155, 2986, 3145
Ppu21I YACGTR 2 cut(s) 2038, 3995
PscI ACATGT 1 cut(s) 2922
Psp6I CCWGG 8 cut(s) 161, 647, 894, 2305, 2898, 3906, 3931, 4026
PspCI CACGTG 1 cut(s) 3995
PspFI CCCAGC 3 cut(s) 65, 1073, 2272
PspGI CCWGG 8 cut(s) 161, 647, 894, 2305, 2898, 3906, 3931, 4026
PspN4I GGNNCC 6 cut(s) 105, 1376, 1519, 3059, 3858, 4064
PspPI GGNCC 8 cut(s) 670, 1375, 1752, 1778, 2725, 2896, 3904, 3935
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 1 cut(s) 3074
PsuI RGATCY 5 cut(s) 223, 1732, 2482, 2586, 3354
PvuII CAGCTG 1 cut(s) 1682
RsaI GTAC 4 cut(s) 552, 942, 2656, 3492
RsaNI GTAC 4 cut(s) 551, 941, 2655, 3491
RseI CAYNNNNRTG 4 cut(s) 2141, 2446, 4019, 4095
SapI GCTCTTC 2 cut(s) 2832, 3552
Sau96I GGNCC 8 cut(s) 670, 1375, 1752, 1778, 2725, 2896, 3904, 3935
ScaI AGTACT 1 cut(s) 2656
SchI GAGTC 4 cut(s) 167, 2155, 2987, 3146
ScrFI CCNGG 9 cut(s) 163, 649, 896, 1933, 2307, 2900, 3908, 3933, 4028
SduI GDGCHC 7 cut(s) 84, 415, 789, 1104, 2086, 2827, 3052
SexAI ACCWGGT 1 cut(s) 2898
SfaNI GCATC 5 cut(s) 316, 1291, 1896, 3467, 3703
SfcI CTRYAG 1 cut(s) 1683
SfuI TTCGAA 2 cut(s) 2266, 3235
SinI GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
SmiMI CAYNNNNRTG 4 cut(s) 2141, 2446, 4019, 4095
SmlI CTYRAG 2 cut(s) 2121, 3383
SmoI CTYRAG 2 cut(s) 2121, 3383
SnaBI TACGTA 1 cut(s) 2038
SpeI ACTAGT 1 cut(s) 3794
SseBI AGGCCT 3 cut(s) 1136, 2050, 3533
SsiI CCGC 7 cut(s) 35, 43, 1298, 2284, 3388, 3511, 3854
SspI AATATT 4 cut(s) 562, 1253, 2223, 3945
StuI AGGCCT 3 cut(s) 1136, 2050, 3533
StyD4I CCNGG 9 cut(s) 161, 647, 894, 1931, 2305, 2898, 3906, 3931, 4026
StyI CCWWGG 4 cut(s) 522, 659, 2728, 3723
TaaI ACNGT 4 cut(s) 628, 2240, 2633, 3490
TaiI ACGT 4 cut(s) 244, 2040, 3285, 3997
TaqII GACCGA 1 cut(s) 1392
TatI WGTACW 2 cut(s) 940, 2654
TauI GCSGC 2 cut(s) 37, 3857
TseFI GTSAC 6 cut(s) 1153, 1508, 1708, 2935, 3070, 3621
Tsp45I GTSAC 6 cut(s) 1153, 1508, 1708, 2935, 3070, 3621
TspGWI ACGGA 6 cut(s) 128, 494, 629, 860, 1765, 3437
Van91I CCANNNNNTGG 2 cut(s) 1118, 1370
VneI GTGCAC 2 cut(s) 785, 3048
VpaK11BI GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
XapI RAATTY 6 cut(s) 1000, 1789, 2196, 3659, 3693, 4076
XbaI TCTAGA 2 cut(s) 280, 700
XceI RCATGY 4 cut(s) 1639, 1837, 2926, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3301
XmiI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
XmnI GAANNNNTTC 3 cut(s) 696, 863, 3282
ZrmI AGTACT 1 cut(s) 2656
Zsp2I ATGCAT 1 cut(s) 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.