Rh1CG007400

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002059
Physical Location & Seq
Forward (+)
1110983 .. 1130616
19634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG007400.1

Sequence Viewer

Length: 5415 bp
ATGGCATATAGAAGGGGCTTCGACCAGCAGCAACCGCAGAGGCGGATTCTGCGGACTCAGACTGCCGGCGCCGGCAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCCAGTGGCCGTGGTGTTCGCCAGTTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGACTTTTTATCGAGATTACTACAAAAAATACATTCAAGATTTGCACAGGACTACTGATAAACCTGATCGTGCACAACTGACAAAGTCATACAAAACTGCTTCTATTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTTCCTCTTGATCGTGATAGTCGAAATCAGGCAATCATGAGATATCCCGAGATTCTAGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGCCGAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTATTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACAGTAGTGATGAAGAAGCTTTTCAAAAATTACAAAAAATGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTAGAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCCGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACCTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACCATACACCGGGGATCGATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTACAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCGCAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCTGTGACTTATGCATTTAGCTGGAAAAATCCCTCTGGGTTTGCACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTGTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGCATCTTGAAAGATCAAACTATAGGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTGGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCATCTAATATAGAGAATGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCACTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGACCCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCAGTTAGAGAGTGCTATCTCTCATTTAAAAGTATTATCAATGTTTTAGTCCAAGGAGAACGCGAAAAGATGGTTATAGATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACACTGACGAAGGAAGTTAAATTGAGTGCTCTCCCAAGCCTCCATGAACAATTTATTAAGCTAATTGATTATCTGTTGAAAAACAAAAGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGAAGATGAAATTACCACCTTGGTAGATTCAAACCATGGTGGATCATATGGAAACGATGGGGGAATGAGACCTCTTAATCAACAAGATACATACTTCAGTGAACTGAATTTCCCAGTGACGCAACAATCAGAGGCTTGGAAAGAAAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGATATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCAGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAACGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACCAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAACTCAGAGCAAGCGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTACAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGAAAGGCCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCCCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCATTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTCGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAACGGTGAGCAGACACTGAGTCGTGACATCTATAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTCTAATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTTGGTACAAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACTGTACAGATATGGTTCATGGTTGGAACATGGCTTTTCGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATTGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTATTCTGGGATGCGCGGTATCGTTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAGAGAACAAAAGTATCCTGGTATATTTGAGTACCTTTTATGCATTTTCAACCTTAAAAAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1804

Amino Acids

208.97

Weight (kDa)

9.04

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 173 6.3e-16 Vta1 like
FKS1_dom1 PF14288 319 - 431 8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 854 - 1043 7.7e-63 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1141 5.5e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1145 - 1760 1.1e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1538
Acc16I TGCGCA 1 cut(s) 1593
Acc36I ACCTGC 1 cut(s) 4331
AccB1I GGYRCC 2 cut(s) 68, 1425
AccB7I CCANNNNNTGG 1 cut(s) 1121
AccBSI CCGCTC 1 cut(s) 4629
AccI GTMKAC 3 cut(s) 1988, 3831, 5313
AccII CGCG 3 cut(s) 1970, 2742, 5271
AclWI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3881
AcoI YGGCCR 3 cut(s) 241, 665, 4246
AcsI RAATTY 8 cut(s) 2199, 2393, 2435, 2763, 3061, 3659, 4076, 4934
AcuI CTGAAG 4 cut(s) 698, 3034, 3426, 3512
AcvI CACGTG 1 cut(s) 3995
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 4787
AfeI AGCGCT 1 cut(s) 3519
AflII CTTAAG 1 cut(s) 4321
AflIII ACRYGT 2 cut(s) 2925, 3992
AhdI GACNNNNNGTC 2 cut(s) 4412, 4479
AhlI ACTAGT 3 cut(s) 2465, 3794, 4184
AjuI GAANNNNNNNTTGG 4 cut(s) 995, 1027, 1777, 1809
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 4 cut(s) 3048, 3080, 4754, 4786
Alw21I GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
Alw26I GTCTC 4 cut(s) 1932, 3016, 3334, 4175
Alw44I GTGCAC 2 cut(s) 414, 2087
AlwI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3881
AlwNI CAGNNNCTG 2 cut(s) 488, 4477
Ama87I CYCGRG 2 cut(s) 614, 2124
Aor51HI AGCGCT 1 cut(s) 3519
ApaLI GTGCAC 2 cut(s) 414, 2087
ApoI RAATTY 8 cut(s) 2199, 2393, 2435, 2763, 3061, 3659, 4076, 4934
AseI ATTAAT 1 cut(s) 4799
Asp700I GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
AspLEI GCGC 7 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304
AspS9I GGNCC 8 cut(s) 1781, 2899, 2947, 3372, 3904, 3935, 4690, 4816
AsuC2I CCSGG 2 cut(s) 1078, 1375
AsuHPI GGTGA 8 cut(s) 1112, 1139, 1547, 3293, 3722, 4478, 4534, 5327
AsuII TTCGAA 2 cut(s) 2018, 4666
AsuNHI GCTAGC 3 cut(s) 1072, 2576, 4217
AvaI CYCGRG 2 cut(s) 614, 2124
AvaII GGWCC 6 cut(s) 1781, 2899, 2947, 3372, 3904, 3935
AxyI CCTNAGG 1 cut(s) 2337
BaeGI GKGCMC 2 cut(s) 418, 2091
BaeI ACNNNNGTAYC 2 cut(s) 5344, 5377
BalI TGGCCA 1 cut(s) 4248
BanI GGYRCC 2 cut(s) 68, 1425
BanII GRGCYC 1 cut(s) 2167
BarI GAAGNNNNNNTAC 4 cut(s) 2198, 2230, 3166, 3198
BauI CACGAG 3 cut(s) 333, 3717, 5243
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 4 cut(s) 702, 3285, 3435, 3807
Bbv12I GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
BbvCI CCTCAGC 1 cut(s) 501
BceAI ACGGC 3 cut(s) 73, 228, 812
BciVI GTATCC 2 cut(s) 2185, 5372
BclI TGATCA 4 cut(s) 814, 1485, 2782, 4314
BcnI CCSGG 2 cut(s) 1078, 1375
BcoDI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BcuI ACTAGT 3 cut(s) 2465, 3794, 4184
BfmI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
BfoI RGCGCY 2 cut(s) 72, 3521
BfrI CTTAAG 1 cut(s) 4321
BfuAI ACCTGC 1 cut(s) 4331
BfuI GTATCC 2 cut(s) 2185, 5372
BglII AGATCT 2 cut(s) 3559, 4428
BmcAI AGTACT 2 cut(s) 896, 4651
Bme18I GGWCC 6 cut(s) 1781, 2899, 2947, 3372, 3904, 3935
BmeRI GACNNNNNGTC 2 cut(s) 4412, 4479
BmeT110I CYCGRG 2 cut(s) 614, 2124
BmgT120I GGNCC 8 cut(s) 1781, 2899, 2947, 3372, 3904, 3935, 4690, 4816
BmiI GGNNCC 8 cut(s) 70, 1427, 2166, 2948, 3858, 3937, 4772, 4818
BmrI ACTGGG 1 cut(s) 3062
BmsI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
BmtI GCTAGC 3 cut(s) 1076, 2580, 4221
BmuI ACTGGG 1 cut(s) 3062
BpiI GAAGAC 4 cut(s) 702, 3285, 3435, 3807
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 1400, 1432
BpmI CTGGAG 1 cut(s) 2297
Bpu10I CCTNAGC 4 cut(s) 501, 519, 525, 4121
Bpu14I TTCGAA 2 cut(s) 2018, 4666
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 2 cut(s) 1078, 1375
Bsa29I ATCGAT 1 cut(s) 1381
BsaAI YACGTR 2 cut(s) 3995, 4361
BsaBI GATNNNNATC 3 cut(s) 2299, 3558, 3915
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 2 cut(s) 1932, 3016
BsaJI CCNNGG 8 cut(s) 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BsaWI WCCGGW 1 cut(s) 3510
BsaXI ACNNNNNCTCC 6 cut(s) 1072, 1102, 3122, 3152, 4415, 4445
Bse118I RCCGGY 4 cut(s) 65, 71, 1875, 2253
Bse1I ACTGG 9 cut(s) 237, 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317
Bse21I CCTNAGG 1 cut(s) 2337
Bse3DI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
Bse8I GATNNNNATC 3 cut(s) 2299, 3558, 3915
BseCI ATCGAT 1 cut(s) 1381
BseDI CCNNGG 8 cut(s) 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BseGI GGATG 8 cut(s) 233, 838, 2550, 3154, 3458, 4249, 4417, 5271
BseJI GATNNNNATC 3 cut(s) 2299, 3558, 3915
BseMI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
BseNI ACTGG 9 cut(s) 237, 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317
BseRI GAGGAG 4 cut(s) 518, 1148, 2354, 3849
BseSI GKGCMC 2 cut(s) 418, 2091
BsgI GTGCAG 5 cut(s) 621, 2700, 3207, 4764, 4908
Bsh1236I CGCG 3 cut(s) 1970, 2742, 5271
Bsh1285I CGRYCG 2 cut(s) 3597, 4609
BshNI GGYRCC 2 cut(s) 68, 1425
BshVI ATCGAT 1 cut(s) 1381
BsiEI CGRYCG 2 cut(s) 3597, 4609
BsiHKAI GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
BsiHKCI CYCGRG 2 cut(s) 614, 2124
BsiSI CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511
BslFI GGGAC 3 cut(s) 2382, 2504, 2960
BsmAI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BsmFI GGGAC 3 cut(s) 2382, 2504, 2960
BsmI GAATGC 6 cut(s) 175, 1028, 1074, 1219, 1962, 4049
Bso31I GGTCTC 2 cut(s) 1932, 3016
BsoBI CYCGRG 2 cut(s) 614, 2124
Bsp119I TTCGAA 2 cut(s) 2018, 4666
Bsp1286I GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
Bsp1407I TGTACA 1 cut(s) 5065
Bsp19I CCATGG 1 cut(s) 2989
BspDI ATCGAT 1 cut(s) 1381
BspFNI CGCG 3 cut(s) 1970, 2742, 5271
BspHI TCATGA 3 cut(s) 603, 2079, 4381
BspLI GGNNCC 8 cut(s) 70, 1427, 2166, 2948, 3858, 3937, 4772, 4818
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 1 cut(s) 4331
BspOI GCTAGC 3 cut(s) 1076, 2580, 4221
BspPI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3881
BspT104I TTCGAA 2 cut(s) 2018, 4666
BspT107I GGYRCC 2 cut(s) 68, 1425
BspTI CTTAAG 1 cut(s) 4321
BspTNI GGTCTC 2 cut(s) 1932, 3016
BsrBI CCGCTC 1 cut(s) 4629
BsrDI GCAATG 4 cut(s) 1488, 1584, 4733, 5297
BsrFI RCCGGY 4 cut(s) 65, 71, 1875, 2253
BsrGI TGTACA 1 cut(s) 5065
BsrI ACTGG 9 cut(s) 237, 256, 2554, 3068, 3890, 4144, 4909, 5159, 5317
BssAI RCCGGY 4 cut(s) 65, 71, 1875, 2253
BssECI CCNNGG 8 cut(s) 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BssNAI GTATAC 1 cut(s) 1989
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 3 cut(s) 333, 3717, 5243
BssT1I CCWWGG 3 cut(s) 2731, 2973, 2989
Bst1107I GTATAC 1 cut(s) 1989
Bst2BI CACGAG 3 cut(s) 333, 3717, 5243
Bst6I CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
BstACI GRCGYC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 4321
BstAPI GCANNNNNTGC 2 cut(s) 179, 2438
BstAUI TGTACA 1 cut(s) 5065
BstBAI YACGTR 2 cut(s) 3995, 4361
BstBI TTCGAA 2 cut(s) 2018, 4666
BstDSI CCRYGG 2 cut(s) 244, 2989
BstENI CCTNNNNNAGG 1 cut(s) 1651
BstF5I GGATG 8 cut(s) 233, 838, 2550, 3154, 3458, 4249, 4417, 5271
BstFNI CGCG 3 cut(s) 1970, 2742, 5271
BstH2I RGCGCY 2 cut(s) 72, 3521
BstHHI GCGC 7 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304
BstMAI GTCTC 4 cut(s) 1932, 3016, 3334, 4175
BstMCI CGRYCG 2 cut(s) 3597, 4609
BstNSI RCATGY 3 cut(s) 1642, 1840, 2929
BstSFI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
BstSLI GKGCMC 2 cut(s) 418, 2091
BstUI CGCG 3 cut(s) 1970, 2742, 5271
BstV2I GAAGAC 4 cut(s) 702, 3285, 3435, 3807
BstX2I RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
BstXI CCANNNNNNTGG 4 cut(s) 3369, 3689, 3817, 5021
BstYI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
BstZ17I GTATAC 1 cut(s) 1989
Bsu15I ATCGAT 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 2337
BsuI GTATCC 2 cut(s) 2185, 5372
BsuTUI ATCGAT 1 cut(s) 1381
BtgI CCRYGG 2 cut(s) 244, 2989
BtsCI GGATG 8 cut(s) 233, 838, 2550, 3154, 3458, 4249, 4417, 5271
BtsI GCAGTG 2 cut(s) 2303, 4150
BveI ACCTGC 1 cut(s) 4331
CaiI CAGNNNCTG 2 cut(s) 488, 4477
CciI TCATGA 3 cut(s) 603, 2079, 4381
CfoI GCGC 7 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304
Cfr10I RCCGGY 4 cut(s) 65, 71, 1875, 2253
Cfr13I GGNCC 8 cut(s) 1781, 2899, 2947, 3372, 3904, 3935, 4690, 4816
ClaI ATCGAT 1 cut(s) 1381
CseI GACGC 1 cut(s) 3082
CsiI ACCWGGT 1 cut(s) 2901
DinI GGCGCC 1 cut(s) 70
DraI TTTAAA 3 cut(s) 262, 1546, 2707
DraIII CACNNNGTG 1 cut(s) 4787
DrdI GACNNNNNNGTC 1 cut(s) 1538
DriI GACNNNNNGTC 2 cut(s) 4412, 4479
DseDI GACNNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 3 cut(s) 241, 665, 4246
Eam1104I CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
Eam1105I GACNNNNNGTC 2 cut(s) 4412, 4479
EarI CTCTTC 7 cut(s) 1125, 1945, 2126, 2472, 3432, 3765, 4686
EciI GGCGGA 2 cut(s) 58, 3437
Eco130I CCWWGG 3 cut(s) 2731, 2973, 2989
Eco147I AGGCCT 3 cut(s) 1139, 2053, 3533
Eco24I GRGCYC 1 cut(s) 2167
Eco31I GGTCTC 2 cut(s) 1932, 3016
Eco32I GATATC 2 cut(s) 611, 2077
Eco47I GGWCC 6 cut(s) 1781, 2899, 2947, 3372, 3904, 3935
Eco47III AGCGCT 1 cut(s) 3519
Eco57I CTGAAG 4 cut(s) 698, 3034, 3426, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco81I CCTNAGG 1 cut(s) 2337
Eco88I CYCGRG 2 cut(s) 614, 2124
EcoNI CCTNNNNNAGG 1 cut(s) 1651
EcoO109I RGGNCCY 1 cut(s) 2947
EcoRV GATATC 2 cut(s) 611, 2077
EcoT14I CCWWGG 3 cut(s) 2731, 2973, 2989
EcoT22I ATGCAT 4 cut(s) 205, 1723, 1962, 5392
EcoT38I GRGCYC 1 cut(s) 2167
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 3 cut(s) 2731, 2973, 2989
FaqI GGGAC 3 cut(s) 2382, 2504, 2960
FauI CCCGC 1 cut(s) 3946
FauNDI CATATG 3 cut(s) 1047, 3001, 3667
FbaI TGATCA 4 cut(s) 814, 1485, 2782, 4314
FblI GTMKAC 3 cut(s) 1988, 3831, 5313
FokI GGATG 8 cut(s) 220, 845, 2537, 3161, 3445, 4236, 4424, 5278
FriOI GRGCYC 1 cut(s) 2167
FspI TGCGCA 1 cut(s) 1593
GlaI GCGC 7 cut(s) 70, 1593, 1969, 2675, 3519, 5270, 5303
GsuI CTGGAG 1 cut(s) 2297
HaeII RGCGCY 2 cut(s) 72, 3521
HapII CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511
HgaI GACGC 1 cut(s) 3082
HhaI GCGC 7 cut(s) 71, 1594, 1970, 2676, 3520, 5271, 5304
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 7 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302
HinP1I GCGC 7 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302
HincII GTYRAC 3 cut(s) 1345, 3652, 4575
HindII GTYRAC 3 cut(s) 1345, 3652, 4575
HindIII AAGCTT 4 cut(s) 863, 1586, 3926, 4302
HpaI GTTAAC 1 cut(s) 4575
HpaII CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511
HphI GGTGA 8 cut(s) 1112, 1139, 1547, 3293, 3722, 4478, 4534, 5327
HpyCH4IV ACGT 4 cut(s) 280, 3282, 3994, 4360
HpySE526I ACGT 4 cut(s) 280, 3282, 3994, 4360
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 7 cut(s) 69, 1592, 1968, 2674, 3518, 5269, 5302
KasI GGCGCC 1 cut(s) 68
KroI GCCGGC 4 cut(s) 65, 71, 1875, 2253
KroNI GCCGGC 4 cut(s) 67, 73, 1877, 2255
Ksp22I TGATCA 4 cut(s) 814, 1485, 2782, 4314
KspAI GTTAAC 1 cut(s) 4575
LmnI GCTCC 8 cut(s) 136, 760, 1197, 1455, 2170, 3862, 4126, 5107
LweI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
MabI ACCWGGT 1 cut(s) 2901
MaeII ACGT 4 cut(s) 280, 3282, 3994, 4360
MbiI CCGCTC 1 cut(s) 4629
MfeI CAATTG 3 cut(s) 2169, 3873, 4811
MflI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
MhlI GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
MlsI TGGCCA 1 cut(s) 4248
MluNI TGGCCA 1 cut(s) 4248
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 5 cut(s) 49, 2266, 3146, 4489, 4846
MmeI TCCRAC 6 cut(s) 72, 810, 1927, 3219, 3887, 5065
Mox20I TGGCCA 1 cut(s) 4248
Mph1103I ATGCAT 4 cut(s) 205, 1723, 1962, 5392
MroNI GCCGGC 4 cut(s) 65, 71, 1875, 2253
MroXI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
MscI TGGCCA 1 cut(s) 4248
MslI CAYNNNNRTG 4 cut(s) 2084, 2446, 4254, 4884
Msp20I TGGCCA 1 cut(s) 4248
MspA1I CMGCKG 2 cut(s) 952, 1685
MspCI CTTAAG 1 cut(s) 4321
MspI CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3511
MunI CAATTG 3 cut(s) 2169, 3873, 4811
Mva1269I GAATGC 6 cut(s) 175, 1028, 1074, 1219, 1962, 4049
MvnI CGCG 3 cut(s) 1970, 2742, 5271
NaeI GCCGGC 4 cut(s) 67, 73, 1877, 2255
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 2 cut(s) 1078, 1375
NcoI CCATGG 1 cut(s) 2989
NdeI CATATG 3 cut(s) 1047, 3001, 3667
NgoMIV GCCGGC 4 cut(s) 65, 71, 1875, 2253
NheI GCTAGC 3 cut(s) 1072, 2576, 4217
NlaIV GGNNCC 8 cut(s) 70, 1427, 2166, 2948, 3858, 3937, 4772, 4818
NmeAIII GCCGAG 1 cut(s) 1915
NmuCI GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
NsbI TGCGCA 1 cut(s) 1593
NsiI ATGCAT 4 cut(s) 205, 1723, 1962, 5392
NspI RCATGY 3 cut(s) 1642, 1840, 2929
NspV TTCGAA 2 cut(s) 2018, 4666
OliI CACNNNNGTG 1 cut(s) 2446
PaeR7I CTCGAG 1 cut(s) 2124
PagI TCATGA 3 cut(s) 603, 2079, 4381
PceI AGGCCT 3 cut(s) 1139, 2053, 3533
PciI ACATGT 1 cut(s) 2925
PcsI WCGNNNNNNNCGW 2 cut(s) 586, 4663
PctI GAATGC 6 cut(s) 175, 1028, 1074, 1219, 1962, 4049
PdiI GCCGGC 4 cut(s) 67, 73, 1877, 2255
PdmI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
PflFI GACNNNGTC 1 cut(s) 427
PflMI CCANNNNNTGG 1 cut(s) 1121
PfoI TCCNGGA 2 cut(s) 3537, 3931
Ple19I CGATCG 1 cut(s) 3597
PleI GAGTC 5 cut(s) 49, 2266, 3145, 4488, 4846
PluTI GGCGCC 1 cut(s) 72
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 5 cut(s) 49, 2266, 3145, 4488, 4846
Ppu21I YACGTR 2 cut(s) 3995, 4361
PpuMI RGGWCCY 1 cut(s) 2947
PscI ACATGT 1 cut(s) 2925
PshBI ATTAAT 1 cut(s) 4799
Psp5II RGGWCCY 1 cut(s) 2947
PspCI CACGTG 1 cut(s) 3995
PspN4I GGNNCC 8 cut(s) 70, 1427, 2166, 2948, 3858, 3937, 4772, 4818
PspPI GGNCC 8 cut(s) 1781, 2899, 2947, 3372, 3904, 3935, 4690, 4816
PspPPI RGGWCCY 1 cut(s) 2947
PspXI VCTCGAGB 1 cut(s) 2124
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 2 cut(s) 488, 4477
PsuI RGATCY 7 cut(s) 226, 2403, 2482, 2583, 3102, 3559, 4428
PsyI GACNNNGTC 1 cut(s) 427
PvuI CGATCG 1 cut(s) 3597
PvuII CAGCTG 1 cut(s) 1685
RseI CAYNNNNRTG 4 cut(s) 2084, 2446, 4254, 4884
Sau96I GGNCC 8 cut(s) 1781, 2899, 2947, 3372, 3904, 3935, 4690, 4816
ScaI AGTACT 2 cut(s) 896, 4651
SchI GAGTC 5 cut(s) 49, 2266, 3146, 4489, 4846
SduI GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
SexAI ACCWGGT 1 cut(s) 2901
SfaNI GCATC 8 cut(s) 319, 1294, 1888, 1899, 3467, 3703, 5256, 5257
SfcI CTRYAG 7 cut(s) 1176, 1686, 1897, 2589, 4492, 4707, 4948
SfoI GGCGCC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 2124
SfuI TTCGAA 2 cut(s) 2018, 4666
SinI GGWCC 6 cut(s) 1781, 2899, 2947, 3372, 3904, 3935
SlaI CTCGAG 1 cut(s) 2124
SmiMI CAYNNNNRTG 4 cut(s) 2084, 2446, 4254, 4884
SmlI CTYRAG 3 cut(s) 1006, 2124, 4321
SmoI CTYRAG 3 cut(s) 1006, 2124, 4321
SpeI ACTAGT 3 cut(s) 2465, 3794, 4184
SseBI AGGCCT 3 cut(s) 1139, 2053, 3533
SspDI GGCGCC 1 cut(s) 68
SspI AATATT 1 cut(s) 565
StuI AGGCCT 3 cut(s) 1139, 2053, 3533
StyI CCWWGG 3 cut(s) 2731, 2973, 2989
TaiI ACGT 4 cut(s) 283, 3285, 3997, 4363
TatI WGTACW 6 cut(s) 894, 1520, 2181, 4649, 4893, 5065
TauI GCSGC 2 cut(s) 3625, 3857
TseFI GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
Tsp45I GTSAC 5 cut(s) 1711, 2938, 3070, 4372, 4484
TspGWI ACGGA 4 cut(s) 131, 3143, 4763, 4878
Tth111I GACNNNGTC 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 1121
Vha464I CTTAAG 1 cut(s) 4321
VneI GTGCAC 2 cut(s) 414, 2087
VpaK11BI GGWCC 6 cut(s) 1781, 2899, 2947, 3372, 3904, 3935
VspI ATTAAT 1 cut(s) 4799
XagI CCTNNNNNAGG 1 cut(s) 1651
XapI RAATTY 8 cut(s) 2199, 2393, 2435, 2763, 3061, 3659, 4076, 4934
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 3 cut(s) 1642, 1840, 2929
XcmI CCANNNNNNNNNTGG 1 cut(s) 3301
XhoI CTCGAG 1 cut(s) 2124
XmiI GTMKAC 3 cut(s) 1988, 3831, 5313
XmnI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4077
ZrmI AGTACT 2 cut(s) 896, 4651
Zsp2I ATGCAT 4 cut(s) 205, 1723, 1962, 5392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.