RchiOBHm_Chr1g0313341

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002059
Physical Location & Seq
Forward (+)
437116 .. 453950
16835 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54416

Sequence Viewer

Length: 4386 bp
ATGGCATATAGAAGGGGCTTTGACCAGCAGCATCCGCAGAGGCGGATTCTGCGGACTCAGACTGCCGGCCCCGGCAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCCCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAAGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGATTAGATCCCACATCCAGTGGCCGTGGTGTTCGCCAGTTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCACAGTACTGCTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTATTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTTCCTCTTGATCGTGATAGTCGAAATCAGGCAATCATGAGATATCCCGAGATTCTTGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGCCGAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTATTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACAGTAGTGATGAAGAAACTTTTCAAAAATTACAAAAAATGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTAGAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCCGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGGAATTATGATGACCTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACCATACACCGGGGATCGATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTACAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCGCAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCCGTGACTTATGCATATAGTTGGAAAAATCCCTCTGGGTTTGCACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTGTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGTTTCTTGAAAGATCAAACTATAGGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACTCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTGGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCAACTAATATAGAGAATGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCACTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGAACCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCAGTTAGAGAGTGCTATCTTTCGTTTAGAAGTATTATCAATGTTTTAGTCCAAGGAGAACGCGAAAAGATGGTTATAAATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACACTGACGAAGGATGTTAAATTGAGTGCTCTCCCAAGCCTCCACGAACAATTTATTAAGCTAATTGATTATCTGTTGAAAAACAAAAGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGAAGATGAAATTACCACCTTGGTAGATTCAAACCATGGTGGATCATATGGAAATGATGGGGGAATGAGACCTCTTAATCAACAAGATACATACTTCAGTGAACTGAATTTCCCAGTGACGCAACAATCAGAAGCTTGGAAAGAAAAGATCCGAAGGCTGCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCGTCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGACATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCGGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAATGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACTAACTTTCTTGAACGGGTCAAATGTGCTGAAGATGAACTCAGAGCAAACGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTATAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGATAGGCCGTTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCCCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTTTCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGTAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGCATTCTCATGGCTCTGAAACTGTTTCAAGTCTTTATTTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1461

Amino Acids

169.21

Weight (kDa)

8.85

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 173 1.2e-16 Vta1 like
FKS1_dom1 PF14288 319 - 431 6.1e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 854 - 1043 7.2e-63 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1141 2e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1144 - 1450 2.2e-103 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2756
AasI GACNNNNNNGTC 1 cut(s) 1538
Acc16I TGCGCA 1 cut(s) 1593
AccB1I GGYRCC 1 cut(s) 1425
AccB7I CCANNNNNTGG 1 cut(s) 1121
AccI GTMKAC 2 cut(s) 1988, 3828
AccII CGCG 2 cut(s) 1970, 2742
AciI CCGC 8 cut(s) 35, 43, 52, 267, 952, 3445, 3851, 3936
AclWI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3878
AcoI YGGCCR 3 cut(s) 241, 665, 4243
AcsI RAATTY 7 cut(s) 2199, 2393, 2435, 2763, 3061, 3656, 4073
AcuI CTGAAG 4 cut(s) 698, 3034, 3423, 3509
AcvI CACGTG 1 cut(s) 3992
AfaI GTAC 5 cut(s) 394, 896, 1522, 2183, 3489
AfeI AGCGCT 1 cut(s) 3516
AflII CTTAAG 1 cut(s) 4318
AflIII ACRYGT 2 cut(s) 2925, 3989
AhlI ACTAGT 3 cut(s) 2465, 3791, 4181
AjnI CCWGG 8 cut(s) 662, 1691, 2901, 3303, 3534, 3903, 3928, 4023
AjuI GAANNNNNNNTTGG 4 cut(s) 995, 1027, 1777, 1809
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 2 cut(s) 3048, 3080
Alw21I GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
Alw26I GTCTC 4 cut(s) 1932, 3016, 3334, 4172
Alw44I GTGCAC 2 cut(s) 414, 2087
AlwI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3878
AlwNI CAGNNNCTG 1 cut(s) 488
Ama87I CYCGRG 2 cut(s) 614, 2124
Aor51HI AGCGCT 1 cut(s) 3516
ApaLI GTGCAC 2 cut(s) 414, 2087
ApoI RAATTY 7 cut(s) 2199, 2393, 2435, 2763, 3061, 3656, 4073
Asp700I GAANNNNTTC 4 cut(s) 699, 866, 3282, 4074
AspLEI GCGC 4 cut(s) 1594, 1970, 2676, 3517
AspS9I GGNCC 6 cut(s) 68, 1781, 2899, 2947, 3901, 3932
AsuC2I CCSGG 3 cut(s) 72, 1078, 1375
AsuHPI GGTGA 5 cut(s) 1112, 1139, 1547, 3293, 3719
AsuII TTCGAA 1 cut(s) 2018
AsuNHI GCTAGC 3 cut(s) 1072, 2576, 4214
AvaI CYCGRG 2 cut(s) 614, 2124
AvaII GGWCC 5 cut(s) 1781, 2899, 2947, 3901, 3932
AxyI CCTNAGG 1 cut(s) 2337
BaeGI GKGCMC 2 cut(s) 418, 2091
BalI TGGCCA 1 cut(s) 4245
BanI GGYRCC 1 cut(s) 1425
BanII GRGCYC 1 cut(s) 2167
BarI GAAGNNNNNNTAC 4 cut(s) 2198, 2230, 3166, 3198
BauI CACGAG 2 cut(s) 333, 3714
BbrPI CACGTG 1 cut(s) 3992
BbsI GAAGAC 4 cut(s) 702, 3285, 3432, 3804
Bbv12I GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
BbvCI CCTCAGC 1 cut(s) 501
BccI CCATC 9 cut(s) 1377, 1766, 2219, 2743, 3005, 3317, 3760, 4085, 4254
BceAI ACGGC 4 cut(s) 73, 228, 812, 3604
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 8 cut(s) 664, 1693, 2903, 3305, 3536, 3905, 3930, 4025
BciVI GTATCC 1 cut(s) 2185
BclI TGATCA 4 cut(s) 814, 1485, 2782, 4311
BcnI CCSGG 3 cut(s) 72, 1078, 1375
BcoDI GTCTC 4 cut(s) 1932, 3016, 3334, 4172
BcuI ACTAGT 3 cut(s) 2465, 3791, 4181
BfmI CTRYAG 4 cut(s) 1176, 1686, 1897, 2589
BfoI RGCGCY 1 cut(s) 3518
BfrI CTTAAG 1 cut(s) 4318
BfuI GTATCC 1 cut(s) 2185
BglII AGATCT 1 cut(s) 3556
BmcAI AGTACT 2 cut(s) 394, 896
Bme18I GGWCC 5 cut(s) 1781, 2899, 2947, 3901, 3932
BmeT110I CYCGRG 2 cut(s) 614, 2124
BmgT120I GGNCC 6 cut(s) 68, 1781, 2899, 2947, 3901, 3932
BmiI GGNNCC 6 cut(s) 70, 1427, 2166, 2948, 3855, 3934
BmrI ACTGGG 1 cut(s) 3062
BmsI GCATC 7 cut(s) 40, 319, 1294, 1899, 3124, 3464, 3700
BmtI GCTAGC 3 cut(s) 1076, 2580, 4218
BmuI ACTGGG 1 cut(s) 3062
BpiI GAAGAC 4 cut(s) 702, 3285, 3432, 3804
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 1400, 1432
BpmI CTGGAG 1 cut(s) 2297
Bpu10I CCTNAGC 4 cut(s) 501, 519, 525, 4118
Bpu14I TTCGAA 1 cut(s) 2018
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 3 cut(s) 72, 1078, 1375
Bsa29I ATCGAT 1 cut(s) 1381
BsaAI YACGTR 1 cut(s) 3992
BsaBI GATNNNNATC 3 cut(s) 2299, 3555, 3912
BsaI GGTCTC 2 cut(s) 1932, 3016
BsaJI CCNNGG 9 cut(s) 70, 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BsaWI WCCGGW 1 cut(s) 3507
BsaXI ACNNNNNCTCC 4 cut(s) 1072, 1102, 3122, 3152
Bse118I RCCGGY 3 cut(s) 65, 1875, 2253
Bse1I ACTGG 6 cut(s) 237, 256, 2554, 3068, 3887, 4141
Bse21I CCTNAGG 1 cut(s) 2337
Bse3DI GCAATG 2 cut(s) 1488, 1584
Bse8I GATNNNNATC 3 cut(s) 2299, 3555, 3912
BseBI CCWGG 8 cut(s) 664, 1693, 2903, 3305, 3536, 3905, 3930, 4025
BseCI ATCGAT 1 cut(s) 1381
BseDI CCNNGG 9 cut(s) 70, 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BseGI GGATG 8 cut(s) 31, 233, 838, 2550, 2818, 3154, 3455, 4246
BseJI GATNNNNATC 3 cut(s) 2299, 3555, 3912
BseMI GCAATG 2 cut(s) 1488, 1584
BseNI ACTGG 6 cut(s) 237, 256, 2554, 3068, 3887, 4141
BseRI GAGGAG 4 cut(s) 518, 1148, 2354, 3846
BseSI GKGCMC 2 cut(s) 418, 2091
BsgI GTGCAG 3 cut(s) 621, 2700, 3207
Bsh1236I CGCG 2 cut(s) 1970, 2742
Bsh1285I CGRYCG 1 cut(s) 3594
BshNI GGYRCC 1 cut(s) 1425
BshVI ATCGAT 1 cut(s) 1381
BsiEI CGRYCG 1 cut(s) 3594
BsiHKAI GWGCWC 5 cut(s) 418, 765, 1107, 2091, 2830
BsiHKCI CYCGRG 2 cut(s) 614, 2124
BsiSI CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3508
BslFI GGGAC 3 cut(s) 2382, 2504, 2960
BsmAI GTCTC 4 cut(s) 1932, 3016, 3334, 4172
BsmFI GGGAC 3 cut(s) 2382, 2504, 2960
BsmI GAATGC 7 cut(s) 175, 1028, 1074, 1219, 1962, 4046, 4341
Bso31I GGTCTC 2 cut(s) 1932, 3016
BsoBI CYCGRG 2 cut(s) 614, 2124
Bsp119I TTCGAA 1 cut(s) 2018
Bsp1286I GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
Bsp19I CCATGG 1 cut(s) 2989
BspACI CCGC 8 cut(s) 35, 43, 52, 267, 952, 3445, 3851, 3936
BspDI ATCGAT 1 cut(s) 1381
BspFNI CGCG 2 cut(s) 1970, 2742
BspHI TCATGA 2 cut(s) 603, 2079
BspLI GGNNCC 6 cut(s) 70, 1427, 2166, 2948, 3855, 3934
BspMAI CTGCAG 1 cut(s) 1690
BspOI GCTAGC 3 cut(s) 1076, 2580, 4218
BspPI GGATC 8 cut(s) 221, 1386, 2398, 2490, 2578, 3004, 3097, 3878
BspT104I TTCGAA 1 cut(s) 2018
BspT107I GGYRCC 1 cut(s) 1425
BspTI CTTAAG 1 cut(s) 4318
BspTNI GGTCTC 2 cut(s) 1932, 3016
BsrDI GCAATG 2 cut(s) 1488, 1584
BsrFI RCCGGY 3 cut(s) 65, 1875, 2253
BsrI ACTGG 6 cut(s) 237, 256, 2554, 3068, 3887, 4141
BssAI RCCGGY 3 cut(s) 65, 1875, 2253
BssECI CCNNGG 9 cut(s) 70, 244, 662, 1374, 1692, 1934, 2731, 2973, 2989
BssNAI GTATAC 1 cut(s) 1989
BssSI CACGAG 2 cut(s) 333, 3714
BssT1I CCWWGG 3 cut(s) 2731, 2973, 2989
Bst1107I GTATAC 1 cut(s) 1989
Bst2BI CACGAG 2 cut(s) 333, 3714
Bst2UI CCWGG 8 cut(s) 664, 1693, 2903, 3305, 3536, 3905, 3930, 4025
Bst6I CTCTTC 6 cut(s) 1125, 1945, 2126, 2472, 3429, 3762
BstAFI CTTAAG 1 cut(s) 4318
BstAPI GCANNNNNTGC 3 cut(s) 179, 2438, 3121
BstBAI YACGTR 1 cut(s) 3992
BstBI TTCGAA 1 cut(s) 2018
BstDSI CCRYGG 2 cut(s) 244, 2989
BstENI CCTNNNNNAGG 1 cut(s) 1651
BstF5I GGATG 8 cut(s) 31, 233, 838, 2550, 2818, 3154, 3455, 4246
BstFNI CGCG 2 cut(s) 1970, 2742
BstH2I RGCGCY 1 cut(s) 3518
BstHHI GCGC 4 cut(s) 1594, 1970, 2676, 3517
BstMAI GTCTC 4 cut(s) 1932, 3016, 3334, 4172
BstMCI CGRYCG 1 cut(s) 3594
BstNI CCWGG 8 cut(s) 664, 1693, 2903, 3305, 3536, 3905, 3930, 4025
BstNSI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
BstSFI CTRYAG 4 cut(s) 1176, 1686, 1897, 2589
BstSLI GKGCMC 2 cut(s) 418, 2091
BstUI CGCG 2 cut(s) 1970, 2742
BstV2I GAAGAC 4 cut(s) 702, 3285, 3432, 3804
BstX2I RGATCY 6 cut(s) 226, 2403, 2482, 2583, 3102, 3556
BstXI CCANNNNNNTGG 3 cut(s) 3369, 3686, 3814
BstYI RGATCY 6 cut(s) 226, 2403, 2482, 2583, 3102, 3556
BstZ17I GTATAC 1 cut(s) 1989
Bsu15I ATCGAT 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 2337
BsuI GTATCC 1 cut(s) 2185
BsuTUI ATCGAT 1 cut(s) 1381
BtgI CCRYGG 2 cut(s) 244, 2989
BtsCI GGATG 8 cut(s) 31, 233, 838, 2550, 2818, 3154, 3455, 4246
BtsI GCAGTG 2 cut(s) 2303, 4147
BtsIMutI CAGTG 9 cut(s) 244, 1530, 2303, 2561, 2635, 2801, 3058, 3075, 4147
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 2 cut(s) 603, 2079
CfoI GCGC 4 cut(s) 1594, 1970, 2676, 3517
Cfr10I RCCGGY 3 cut(s) 65, 1875, 2253
Cfr13I GGNCC 6 cut(s) 68, 1781, 2899, 2947, 3901, 3932
ClaI ATCGAT 1 cut(s) 1381
CseI GACGC 1 cut(s) 3082
CsiI ACCWGGT 1 cut(s) 2901
Csp6I GTAC 5 cut(s) 393, 895, 1521, 2182, 3488
CviQI GTAC 5 cut(s) 393, 895, 1521, 2182, 3488
DraI TTTAAA 2 cut(s) 262, 1546
DrdI GACNNNNNNGTC 1 cut(s) 1538
DseDI GACNNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 3 cut(s) 241, 665, 4243
Eam1104I CTCTTC 6 cut(s) 1125, 1945, 2126, 2472, 3429, 3762
EarI CTCTTC 6 cut(s) 1125, 1945, 2126, 2472, 3429, 3762
EciI GGCGGA 2 cut(s) 58, 3434
Eco130I CCWWGG 3 cut(s) 2731, 2973, 2989
Eco147I AGGCCT 3 cut(s) 1139, 2053, 3530
Eco24I GRGCYC 1 cut(s) 2167
Eco31I GGTCTC 2 cut(s) 1932, 3016
Eco32I GATATC 2 cut(s) 611, 2077
Eco47I GGWCC 5 cut(s) 1781, 2899, 2947, 3901, 3932
Eco47III AGCGCT 1 cut(s) 3516
Eco57I CTGAAG 4 cut(s) 698, 3034, 3423, 3509
Eco72I CACGTG 1 cut(s) 3992
Eco81I CCTNAGG 1 cut(s) 2337
Eco88I CYCGRG 2 cut(s) 614, 2124
EcoNI CCTNNNNNAGG 1 cut(s) 1651
EcoO109I RGGNCCY 1 cut(s) 2947
EcoRII CCWGG 8 cut(s) 662, 1691, 2901, 3303, 3534, 3903, 3928, 4023
EcoRV GATATC 2 cut(s) 611, 2077
EcoT14I CCWWGG 3 cut(s) 2731, 2973, 2989
EcoT22I ATGCAT 3 cut(s) 205, 1723, 1962
EcoT38I GRGCYC 1 cut(s) 2167
ErhI CCWWGG 3 cut(s) 2731, 2973, 2989
FalI AAGNNNNNCTT 8 cut(s) 687, 719, 1777, 1809, 3270, 3302, 4285, 4317
FaqI GGGAC 3 cut(s) 2382, 2504, 2960
FauI CCCGC 1 cut(s) 3943
FauNDI CATATG 3 cut(s) 1047, 3001, 3664
FbaI TGATCA 4 cut(s) 814, 1485, 2782, 4311
FblI GTMKAC 2 cut(s) 1988, 3828
FokI GGATG 8 cut(s) 18, 220, 845, 2537, 2825, 3161, 3442, 4233
FriOI GRGCYC 1 cut(s) 2167
FspI TGCGCA 1 cut(s) 1593
GlaI GCGC 4 cut(s) 1593, 1969, 2675, 3516
GsuI CTGGAG 1 cut(s) 2297
HaeII RGCGCY 1 cut(s) 3518
HapII CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3508
HgaI GACGC 1 cut(s) 3082
HhaI GCGC 4 cut(s) 1594, 1970, 2676, 3517
Hin6I GCGC 4 cut(s) 1592, 1968, 2674, 3515
HinP1I GCGC 4 cut(s) 1592, 1968, 2674, 3515
HincII GTYRAC 2 cut(s) 1345, 3649
HindII GTYRAC 2 cut(s) 1345, 3649
HindIII AAGCTT 5 cut(s) 381, 1586, 3087, 3923, 4299
HpaII CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3508
HphI GGTGA 5 cut(s) 1112, 1139, 1547, 3293, 3719
HpyCH4IV ACGT 3 cut(s) 280, 3282, 3991
HpySE526I ACGT 3 cut(s) 280, 3282, 3991
HspAI GCGC 4 cut(s) 1592, 1968, 2674, 3515
KroI GCCGGC 2 cut(s) 65, 2253
KroNI GCCGGC 2 cut(s) 67, 2255
Ksp22I TGATCA 4 cut(s) 814, 1485, 2782, 4311
LmnI GCTCC 6 cut(s) 760, 1197, 1455, 2170, 3859, 4123
LweI GCATC 7 cut(s) 40, 319, 1294, 1899, 3124, 3464, 3700
MabI ACCWGGT 1 cut(s) 2901
MaeII ACGT 3 cut(s) 280, 3282, 3991
MaeIII GTNAC 7 cut(s) 1156, 1711, 2033, 2135, 2938, 3070, 4189
MfeI CAATTG 2 cut(s) 2169, 3870
MflI RGATCY 6 cut(s) 226, 2403, 2482, 2583, 3102, 3556
MhlI GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
MlsI TGGCCA 1 cut(s) 4245
MluNI TGGCCA 1 cut(s) 4245
MlyI GAGTC 3 cut(s) 49, 2266, 3146
MmeI TCCRAC 6 cut(s) 72, 810, 1709, 1927, 3219, 3884
Mox20I TGGCCA 1 cut(s) 4245
Mph1103I ATGCAT 3 cut(s) 205, 1723, 1962
MroNI GCCGGC 2 cut(s) 65, 2253
MroXI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4074
MscI TGGCCA 1 cut(s) 4245
MslI CAYNNNNRTG 4 cut(s) 2084, 2446, 4251, 4346
Msp20I TGGCCA 1 cut(s) 4245
MspA1I CMGCKG 2 cut(s) 952, 1685
MspCI CTTAAG 1 cut(s) 4318
MspI CCGG 7 cut(s) 66, 72, 1077, 1374, 1876, 2254, 3508
MunI CAATTG 2 cut(s) 2169, 3870
Mva1269I GAATGC 7 cut(s) 175, 1028, 1074, 1219, 1962, 4046, 4341
MvaI CCWGG 8 cut(s) 664, 1693, 2903, 3305, 3536, 3905, 3930, 4025
MvnI CGCG 2 cut(s) 1970, 2742
NaeI GCCGGC 2 cut(s) 67, 2255
NciI CCSGG 3 cut(s) 72, 1078, 1375
NcoI CCATGG 1 cut(s) 2989
NdeI CATATG 3 cut(s) 1047, 3001, 3664
NgoMIV GCCGGC 2 cut(s) 65, 2253
NheI GCTAGC 3 cut(s) 1072, 2576, 4214
NlaIV GGNNCC 6 cut(s) 70, 1427, 2166, 2948, 3855, 3934
NmeAIII GCCGAG 1 cut(s) 1915
NmuCI GTSAC 3 cut(s) 1711, 2938, 3070
NsbI TGCGCA 1 cut(s) 1593
NsiI ATGCAT 3 cut(s) 205, 1723, 1962
NspI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
NspV TTCGAA 1 cut(s) 2018
OliI CACNNNNGTG 1 cut(s) 2446
PaeR7I CTCGAG 1 cut(s) 2124
PagI TCATGA 2 cut(s) 603, 2079
PceI AGGCCT 3 cut(s) 1139, 2053, 3530
PciI ACATGT 1 cut(s) 2925
PcsI WCGNNNNNNNCGW 1 cut(s) 586
PctI GAATGC 7 cut(s) 175, 1028, 1074, 1219, 1962, 4046, 4341
PdiI GCCGGC 2 cut(s) 67, 2255
PdmI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4074
PflMI CCANNNNNTGG 1 cut(s) 1121
PfoI TCCNGGA 2 cut(s) 3534, 3928
Ple19I CGATCG 1 cut(s) 3594
PleI GAGTC 3 cut(s) 49, 2266, 3145
PmaCI CACGTG 1 cut(s) 3992
PmlI CACGTG 1 cut(s) 3992
PpsI GAGTC 3 cut(s) 49, 2266, 3145
Ppu21I YACGTR 1 cut(s) 3992
PpuMI RGGWCCY 1 cut(s) 2947
PscI ACATGT 1 cut(s) 2925
PsiI TTATAA 1 cut(s) 2756
Psp5II RGGWCCY 1 cut(s) 2947
Psp6I CCWGG 8 cut(s) 662, 1691, 2901, 3303, 3534, 3903, 3928, 4023
PspCI CACGTG 1 cut(s) 3992
PspGI CCWGG 8 cut(s) 662, 1691, 2901, 3303, 3534, 3903, 3928, 4023
PspN4I GGNNCC 6 cut(s) 70, 1427, 2166, 2948, 3855, 3934
PspPI GGNCC 6 cut(s) 68, 1781, 2899, 2947, 3901, 3932
PspPPI RGGWCCY 1 cut(s) 2947
PspXI VCTCGAGB 1 cut(s) 2124
PstI CTGCAG 1 cut(s) 1690
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 6 cut(s) 226, 2403, 2482, 2583, 3102, 3556
PvuI CGATCG 1 cut(s) 3594
PvuII CAGCTG 1 cut(s) 1685
RsaI GTAC 5 cut(s) 394, 896, 1522, 2183, 3489
RsaNI GTAC 5 cut(s) 393, 895, 1521, 2182, 3488
RseI CAYNNNNRTG 4 cut(s) 2084, 2446, 4251, 4346
Sau96I GGNCC 6 cut(s) 68, 1781, 2899, 2947, 3901, 3932
ScaI AGTACT 2 cut(s) 394, 896
SchI GAGTC 3 cut(s) 49, 2266, 3146
SduI GDGCHC 6 cut(s) 418, 765, 1107, 2091, 2167, 2830
SexAI ACCWGGT 1 cut(s) 2901
SfaNI GCATC 7 cut(s) 40, 319, 1294, 1899, 3124, 3464, 3700
SfcI CTRYAG 4 cut(s) 1176, 1686, 1897, 2589
Sfr274I CTCGAG 1 cut(s) 2124
SfuI TTCGAA 1 cut(s) 2018
SinI GGWCC 5 cut(s) 1781, 2899, 2947, 3901, 3932
SlaI CTCGAG 1 cut(s) 2124
SmiMI CAYNNNNRTG 4 cut(s) 2084, 2446, 4251, 4346
SmlI CTYRAG 3 cut(s) 1006, 2124, 4318
SmoI CTYRAG 3 cut(s) 1006, 2124, 4318
SpeI ACTAGT 3 cut(s) 2465, 3791, 4181
SseBI AGGCCT 3 cut(s) 1139, 2053, 3530
SsiI CCGC 8 cut(s) 35, 43, 52, 267, 952, 3445, 3851, 3936
SspI AATATT 1 cut(s) 565
StuI AGGCCT 3 cut(s) 1139, 2053, 3530
StyI CCWWGG 3 cut(s) 2731, 2973, 2989
TaiI ACGT 3 cut(s) 283, 3285, 3994
TatI WGTACW 4 cut(s) 392, 894, 1520, 2181
TauI GCSGC 1 cut(s) 3854
TscAI CASTG 9 cut(s) 244, 1530, 2310, 2561, 2635, 2808, 3058, 3075, 4147
TseFI GTSAC 3 cut(s) 1711, 2938, 3070
Tsp45I GTSAC 3 cut(s) 1711, 2938, 3070
TspGWI ACGGA 3 cut(s) 131, 3143, 3144
TspRI CASTG 9 cut(s) 244, 1530, 2310, 2561, 2635, 2808, 3058, 3075, 4147
Van91I CCANNNNNTGG 1 cut(s) 1121
Vha464I CTTAAG 1 cut(s) 4318
VneI GTGCAC 2 cut(s) 414, 2087
VpaK11BI GGWCC 5 cut(s) 1781, 2899, 2947, 3901, 3932
XagI CCTNNNNNAGG 1 cut(s) 1651
XapI RAATTY 7 cut(s) 2199, 2393, 2435, 2763, 3061, 3656, 4073
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 4 cut(s) 1642, 1840, 2929, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3301
XhoI CTCGAG 1 cut(s) 2124
XmiI GTMKAC 2 cut(s) 1988, 3828
XmnI GAANNNNTTC 4 cut(s) 699, 866, 3282, 4074
ZrmI AGTACT 2 cut(s) 394, 896
Zsp2I ATGCAT 3 cut(s) 205, 1723, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.