Rh1CG001700

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002059
Physical Location & Seq
Reverse (-)
160596 .. 178991
18396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG001700.1

Sequence Viewer

Length: 5856 bp
ATGGCATATAGAAGGGGCTTCGACCAGCAGCAACCGCAGAGGCGGATTCTGCGGACTCAGACTGCCGGAAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTTGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCACAGTACTACTGCTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTGTTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTTCCTCTTGATCCTGATAGTCGAAATCAGGCAATCATGAGATATCCCGAGATTCATGATACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGCCAAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTGTTGATCGCTAATGTTCACATACGGCAATTACCAAAACCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACAGTAGTGATGAAGAAACTTTTCAAAAATTACAAAAAATGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTACAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATATGGGGAGAGGCTGCCAACTTGAGATTCATGCCGGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCTGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGGAAAGTAGTAACTCCAATATATGATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCGAAGCATTCTCAATGGAGAAATTATGATGACTTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCGATGCGTGCTGATGCTGATTTCTTTTACAAGCCCAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACCATACACGGGGCATCGATGGATTGGCAAAGTCAACTTTGTCGAGATACGCTCATTTTGGCACATTTTCCGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGAAATTAAGTTCAGTGTTTGAGGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCACAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCTTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCTGTGACTTATGCATTTAGTTGGAAAAATCCCTCTGGGTTTGCACAGACTATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTTTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGCATCTTGAAAGATCAAACTATAAGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTAATCTGCTTGTTTCGAAATTAGCCTTCAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTAGAGTTGTTGAGAAGTCGATTTGAATCACTTCCCCGTGCTTTTAATGCTACCTTGGTTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCAACTAATAAAGAGATCGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCAGTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGAACCTCATCCGGTGGCCTCCTTTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGGGCTGTGCTGTTAGAGAGTGCTATCTTTCGTTTAGAAGTATTATCAATGTTTTAGTCGATGGAGAACGCGAAAAGATGGTTATAAATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACGCTGACGAAGGAAGTTAAATTGAGTGCTCTCCCAAGCCTCCACGAACAATTTATTAAGCTAATTGAATATCTGTTGAAAAACGAACGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGAAGATGAAATTCCCACCTTGCTAGATTCAAACCATGGTGGATCATATGGAAACGATGAGGGAATGAGACCCCTTAATCAACGAGATACATACTTCGGTGAACTGAATTTCCCAGTGACATCACAAACAGAGGCTTGGAAAGAAAAGATCCGGAGGCTTCATCTATTGCTTACAGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGAAATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCAGAAGATGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAATGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATCTTTCCAGATGAGTGGACTAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAGCTCAGAGCAAACGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGTAAAGCGCTAGAACTTCAGGCCTTCCTGGACATGGCAAAAGATGAAGTTCTAATGGAAGGGTATAAGGCTGCTGAATCAACGATTGAGGAACATTCAAAGGCTGAAAGGTCGCTATCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACCAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATATTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCTCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTCGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAACGGTGAGCAGACACTGAGTCGTGACATCTACAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTGATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTCGGTACGAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACCGTACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACCGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATCGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTACTCTGGGATGCGTGGTATCATTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAAAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATCGTTGTTTTGTTGCTGATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTTTTGTTTCGACTTGTTAAGGGATGTATGTTTGTTACGCTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTTTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCAGTCGGTTCAAACACTTGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTCGCATTCTTGGCTTGGTTCCCTTTTGTTTCTGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGTCCCCGCGCTGGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1951

Amino Acids

225.3

Weight (kDa)

9.25

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 40 - 169 8.2e-15 Vta1 like
FKS1_dom1 PF14288 318 - 430 1.3e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 853 - 1042 3e-62 Callose synthase 1 domain
Glucan_synthase PF02364 1050 - 1140 4.1e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1144 - 1846 2e-207 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2753
Acc36I ACCTGC 1 cut(s) 4334
AccB7I CCANNNNNTGG 1 cut(s) 1118
AccBSI CCGCTC 1 cut(s) 4632
AccI GTMKAC 3 cut(s) 1985, 3834, 5316
AccII CGCG 4 cut(s) 1967, 2739, 5761, 5838
AccIII TCCGGA 1 cut(s) 3102
AciI CCGC 9 cut(s) 35, 43, 52, 949, 3445, 3857, 3942, 4632, 5836
AclWI GGATC 9 cut(s) 215, 569, 2395, 2487, 2575, 3001, 3094, 3884, 5204
AcoI YGGCCR 4 cut(s) 235, 662, 1286, 4249
AcuI CTGAAG 4 cut(s) 695, 2011, 3423, 3509
AcvI CACGTG 1 cut(s) 3998
AdeI CACNNNGTG 1 cut(s) 4790
AfeI AGCGCT 1 cut(s) 3516
AflII CTTAAG 1 cut(s) 4324
AflIII ACRYGT 2 cut(s) 2922, 3995
AhdI GACNNNNNGTC 2 cut(s) 4415, 4482
AhlI ACTAGT 2 cut(s) 3797, 4187
AjuI GAANNNNNNNTTGG 4 cut(s) 992, 1024, 1774, 1806
AleI CACNNNNGTG 1 cut(s) 2443
AloI GAACNNNNNNTCC 6 cut(s) 1501, 1533, 3045, 3077, 4757, 4789
Alw21I GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2827, 3414, 5535
Alw26I GTCTC 4 cut(s) 1929, 3013, 3331, 4178
Alw44I GTGCAC 2 cut(s) 411, 2084
AlwI GGATC 9 cut(s) 215, 569, 2395, 2487, 2575, 3001, 3094, 3884, 5204
AlwNI CAGNNNCTG 2 cut(s) 485, 4480
Ama87I CYCGRG 2 cut(s) 611, 2121
Aor13HI TCCGGA 1 cut(s) 3102
Aor51HI AGCGCT 1 cut(s) 3516
ApaLI GTGCAC 2 cut(s) 411, 2084
AseI ATTAAT 1 cut(s) 4802
Asp700I GAANNNNTTC 5 cut(s) 696, 863, 2301, 3279, 4080
AspA2I CCTAGG 1 cut(s) 5822
AspLEI GCGC 4 cut(s) 1967, 3517, 5307, 5840
AsuC2I CCSGG 1 cut(s) 5714
AsuII TTCGAA 2 cut(s) 2015, 4669
AsuNHI GCTAGC 3 cut(s) 1069, 2573, 4220
AvaI CYCGRG 2 cut(s) 611, 2121
AvaII GGWCC 7 cut(s) 1778, 2896, 2944, 3907, 3938, 5160, 5831
AvrII CCTAGG 1 cut(s) 5822
AxyI CCTNAGG 1 cut(s) 2334
BaeGI GKGCMC 2 cut(s) 415, 2088
BaeI ACNNNNGTAYC 2 cut(s) 5347, 5380
BalI TGGCCA 2 cut(s) 664, 4251
BanII GRGCYC 2 cut(s) 2164, 3414
BarI GAAGNNNNNNTAC 4 cut(s) 2195, 2227, 3163, 3195
BauI CACGAG 4 cut(s) 327, 3720, 5246, 5673
BbrPI CACGTG 1 cut(s) 3998
BbsI GAAGAC 4 cut(s) 699, 3432, 3810, 4859
Bbv12I GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2827, 3414, 5535
BbvCI CCTCAGC 1 cut(s) 498
BceAI ACGGC 3 cut(s) 67, 222, 809
BciVI GTATCC 2 cut(s) 2182, 5375
BclI TGATCA 5 cut(s) 811, 1482, 2779, 4317, 5394
BcnI CCSGG 1 cut(s) 5714
BcoDI GTCTC 4 cut(s) 1929, 3013, 3331, 4178
BcuI ACTAGT 2 cut(s) 3797, 4187
BfmI CTRYAG 6 cut(s) 1173, 1683, 2586, 4495, 4710, 4951
BfoI RGCGCY 1 cut(s) 3518
BfrI CTTAAG 1 cut(s) 4324
BfuAI ACCTGC 1 cut(s) 4334
BfuI GTATCC 2 cut(s) 2182, 5375
BglII AGATCT 2 cut(s) 3351, 4431
BlnI CCTAGG 1 cut(s) 5822
BmcAI AGTACT 3 cut(s) 388, 893, 4654
Bme18I GGWCC 7 cut(s) 1778, 2896, 2944, 3907, 3938, 5160, 5831
BmeRI GACNNNNNGTC 2 cut(s) 4415, 4482
BmeT110I CYCGRG 2 cut(s) 611, 2121
BmrI ACTGGG 2 cut(s) 1317, 3059
BmtI GCTAGC 3 cut(s) 1073, 2577, 4224
BmuI ACTGGG 2 cut(s) 1317, 3059
BpiI GAAGAC 4 cut(s) 699, 3432, 3810, 4859
BplI GAGNNNNNCTC 4 cut(s) 310, 342, 1397, 1429
Bpu10I CCTNAGC 4 cut(s) 498, 516, 522, 4124
Bpu14I TTCGAA 2 cut(s) 2015, 4669
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 1 cut(s) 5714
Bsa29I ATCGAT 1 cut(s) 1378
BsaAI YACGTR 3 cut(s) 3998, 4364, 5807
BsaBI GATNNNNATC 2 cut(s) 2296, 3918
BsaI GGTCTC 2 cut(s) 1929, 3013
BsaJI CCNNGG 8 cut(s) 238, 659, 1689, 1931, 2325, 2986, 5712, 5822
BsaWI WCCGGW 2 cut(s) 2550, 3102
BsaXI ACNNNNNCTCC 4 cut(s) 1069, 1099, 4418, 4448
Bse118I RCCGGY 2 cut(s) 1872, 2250
Bse1I ACTGG 9 cut(s) 250, 1323, 3065, 3893, 4147, 4912, 5162, 5320, 5593
Bse21I CCTNAGG 1 cut(s) 2334
Bse3DI GCAATG 4 cut(s) 1485, 1581, 4736, 5300
Bse8I GATNNNNATC 2 cut(s) 2296, 3918
BseAI TCCGGA 1 cut(s) 3102
BseCI ATCGAT 1 cut(s) 1378
BseDI CCNNGG 8 cut(s) 238, 659, 1689, 1931, 2325, 2986, 5712, 5822
BseJI GATNNNNATC 2 cut(s) 2296, 3918
BseMI GCAATG 4 cut(s) 1485, 1581, 4736, 5300
BseNI ACTGG 9 cut(s) 250, 1323, 3065, 3893, 4147, 4912, 5162, 5320, 5593
BseRI GAGGAG 4 cut(s) 515, 1145, 2351, 3852
BseSI GKGCMC 2 cut(s) 415, 2088
BseYI CCCAGC 1 cut(s) 1073
BsgI GTGCAG 6 cut(s) 246, 618, 3204, 4767, 4911, 5472
Bsh1236I CGCG 4 cut(s) 1967, 2739, 5761, 5838
Bsh1285I CGRYCG 2 cut(s) 4612, 5718
BshVI ATCGAT 1 cut(s) 1378
BsiEI CGRYCG 2 cut(s) 4612, 5718
BsiHKAI GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2827, 3414, 5535
BsiHKCI CYCGRG 2 cut(s) 611, 2121
BsiSI CCGG 7 cut(s) 66, 1016, 1873, 2251, 2551, 3103, 5714
BslFI GGGAC 4 cut(s) 2379, 2501, 2957, 5817
BsmAI GTCTC 4 cut(s) 1929, 3013, 3331, 4178
BsmFI GGGAC 4 cut(s) 2379, 2501, 2957, 5817
Bso31I GGTCTC 2 cut(s) 1929, 3013
BsoBI CYCGRG 2 cut(s) 611, 2121
Bsp119I TTCGAA 2 cut(s) 2015, 4669
Bsp1286I GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2827, 3414, 5535
Bsp13I TCCGGA 1 cut(s) 3102
Bsp19I CCATGG 1 cut(s) 2986
BspACI CCGC 9 cut(s) 35, 43, 52, 949, 3445, 3857, 3942, 4632, 5836
BspDI ATCGAT 1 cut(s) 1378
BspEI TCCGGA 1 cut(s) 3102
BspFNI CGCG 4 cut(s) 1967, 2739, 5761, 5838
BspHI TCATGA 4 cut(s) 600, 619, 2076, 4384
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 1 cut(s) 4334
BspOI GCTAGC 3 cut(s) 1073, 2577, 4224
BspPI GGATC 9 cut(s) 215, 569, 2395, 2487, 2575, 3001, 3094, 3884, 5204
BspT104I TTCGAA 2 cut(s) 2015, 4669
BspTI CTTAAG 1 cut(s) 4324
BspTNI GGTCTC 2 cut(s) 1929, 3013
BsrBI CCGCTC 1 cut(s) 4632
BsrDI GCAATG 4 cut(s) 1485, 1581, 4736, 5300
BsrFI RCCGGY 2 cut(s) 1872, 2250
BsrI ACTGG 9 cut(s) 250, 1323, 3065, 3893, 4147, 4912, 5162, 5320, 5593
BssAI RCCGGY 2 cut(s) 1872, 2250
BssECI CCNNGG 8 cut(s) 238, 659, 1689, 1931, 2325, 2986, 5712, 5822
BssNAI GTATAC 1 cut(s) 1986
BssSI CACGAG 4 cut(s) 327, 3720, 5246, 5673
BssT1I CCWWGG 3 cut(s) 2325, 2986, 5822
Bst1107I GTATAC 1 cut(s) 1986
Bst2BI CACGAG 4 cut(s) 327, 3720, 5246, 5673
Bst6I CTCTTC 7 cut(s) 1122, 1942, 2123, 2469, 3429, 3768, 4689
BstAFI CTTAAG 1 cut(s) 4324
BstAPI GCANNNNNTGC 2 cut(s) 173, 2435
BstBAI YACGTR 3 cut(s) 3998, 4364, 5807
BstBI TTCGAA 2 cut(s) 2015, 4669
BstDSI CCRYGG 2 cut(s) 238, 2986
BstENI CCTNNNNNAGG 1 cut(s) 1648
BstFNI CGCG 4 cut(s) 1967, 2739, 5761, 5838
BstH2I RGCGCY 1 cut(s) 3518
BstHHI GCGC 4 cut(s) 1967, 3517, 5307, 5840
BstMAI GTCTC 4 cut(s) 1929, 3013, 3331, 4178
BstMCI CGRYCG 2 cut(s) 4612, 5718
BstNSI RCATGY 3 cut(s) 1639, 1837, 2926
BstSFI CTRYAG 6 cut(s) 1173, 1683, 2586, 4495, 4710, 4951
BstSLI GKGCMC 2 cut(s) 415, 2088
BstUI CGCG 4 cut(s) 1967, 2739, 5761, 5838
BstV2I GAAGAC 4 cut(s) 699, 3432, 3810, 4859
BstX2I RGATCY 7 cut(s) 220, 2400, 2479, 2580, 3099, 3351, 4431
BstXI CCANNNNNNTGG 5 cut(s) 3366, 3692, 3820, 5024, 5592
BstYI RGATCY 7 cut(s) 220, 2400, 2479, 2580, 3099, 3351, 4431
BstZ17I GTATAC 1 cut(s) 1986
Bsu15I ATCGAT 1 cut(s) 1378
Bsu36I CCTNAGG 1 cut(s) 2334
BsuI GTATCC 2 cut(s) 2182, 5375
BsuTUI ATCGAT 1 cut(s) 1378
BtgI CCRYGG 2 cut(s) 238, 2986
BtsI GCAGTG 1 cut(s) 4153
BtsIMutI CAGTG 6 cut(s) 1527, 2632, 3072, 4153, 4478, 4587
BveI ACCTGC 1 cut(s) 4334
CaiI CAGNNNCTG 2 cut(s) 485, 4480
CciI TCATGA 4 cut(s) 600, 619, 2076, 4384
CfoI GCGC 4 cut(s) 1967, 3517, 5307, 5840
Cfr10I RCCGGY 2 cut(s) 1872, 2250
ClaI ATCGAT 1 cut(s) 1378
CsiI ACCWGGT 1 cut(s) 2898
DraI TTTAAA 2 cut(s) 256, 1543
DraIII CACNNNGTG 1 cut(s) 4790
DriI GACNNNNNGTC 2 cut(s) 4415, 4482
EaeI YGGCCR 4 cut(s) 235, 662, 1286, 4249
Eam1104I CTCTTC 7 cut(s) 1122, 1942, 2123, 2469, 3429, 3768, 4689
Eam1105I GACNNNNNGTC 2 cut(s) 4415, 4482
EarI CTCTTC 7 cut(s) 1122, 1942, 2123, 2469, 3429, 3768, 4689
EciI GGCGGA 2 cut(s) 58, 3434
Ecl136II GAGCTC 1 cut(s) 3412
Eco130I CCWWGG 3 cut(s) 2325, 2986, 5822
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3530
Eco24I GRGCYC 2 cut(s) 2164, 3414
Eco31I GGTCTC 2 cut(s) 1929, 3013
Eco32I GATATC 2 cut(s) 608, 2074
Eco47I GGWCC 7 cut(s) 1778, 2896, 2944, 3907, 3938, 5160, 5831
Eco47III AGCGCT 1 cut(s) 3516
Eco53kI GAGCTC 1 cut(s) 3412
Eco57I CTGAAG 4 cut(s) 695, 2011, 3423, 3509
Eco72I CACGTG 1 cut(s) 3998
Eco81I CCTNAGG 1 cut(s) 2334
Eco88I CYCGRG 2 cut(s) 611, 2121
EcoICRI GAGCTC 1 cut(s) 3412
EcoNI CCTNNNNNAGG 1 cut(s) 1648
EcoO109I RGGNCCY 3 cut(s) 2944, 5819, 5831
EcoRI GAATTC 1 cut(s) 5750
EcoRV GATATC 2 cut(s) 608, 2074
EcoT14I CCWWGG 3 cut(s) 2325, 2986, 5822
EcoT22I ATGCAT 3 cut(s) 199, 1720, 1959
EcoT38I GRGCYC 2 cut(s) 2164, 3414
ErhI CCWWGG 3 cut(s) 2325, 2986, 5822
FaqI GGGAC 4 cut(s) 2379, 2501, 2957, 5817
FauI CCCGC 2 cut(s) 3949, 5843
FauNDI CATATG 4 cut(s) 1044, 2998, 3670, 5541
FbaI TGATCA 5 cut(s) 811, 1482, 2779, 4317, 5394
FblI GTMKAC 3 cut(s) 1985, 3834, 5316
FriOI GRGCYC 2 cut(s) 2164, 3414
GlaI GCGC 4 cut(s) 1966, 3516, 5306, 5839
GsaI CCCAGC 1 cut(s) 1077
HaeII RGCGCY 1 cut(s) 3518
HapII CCGG 7 cut(s) 66, 1016, 1873, 2251, 2551, 3103, 5714
HhaI GCGC 4 cut(s) 1967, 3517, 5307, 5840
Hin6I GCGC 4 cut(s) 1965, 3515, 5305, 5838
HinP1I GCGC 4 cut(s) 1965, 3515, 5305, 5838
HincII GTYRAC 4 cut(s) 1342, 1396, 3655, 4578
HindII GTYRAC 4 cut(s) 1342, 1396, 3655, 4578
HindIII AAGCTT 5 cut(s) 375, 1583, 3929, 4305, 5616
HpaI GTTAAC 1 cut(s) 4578
HpaII CCGG 7 cut(s) 66, 1016, 1873, 2251, 2551, 3103, 5714
Hpy99I CGWCG 1 cut(s) 5445
HpyCH4IV ACGT 4 cut(s) 274, 3997, 4363, 5806
HpySE526I ACGT 4 cut(s) 274, 3997, 4363, 5806
HspAI GCGC 4 cut(s) 1965, 3515, 5305, 5838
KflI GGGWCCC 1 cut(s) 5831
Kpn2I TCCGGA 1 cut(s) 3102
KroI GCCGGC 2 cut(s) 1872, 2250
KroNI GCCGGC 2 cut(s) 1874, 2252
Ksp22I TGATCA 5 cut(s) 811, 1482, 2779, 4317, 5394
KspAI GTTAAC 1 cut(s) 4578
LmnI GCTCC 8 cut(s) 130, 757, 1194, 1452, 2167, 3865, 4129, 5110
MabI ACCWGGT 1 cut(s) 2898
MaeII ACGT 4 cut(s) 274, 3997, 4363, 5806
MbiI CCGCTC 1 cut(s) 4632
MfeI CAATTG 3 cut(s) 2166, 3876, 4814
MflI RGATCY 7 cut(s) 220, 2400, 2479, 2580, 3099, 3351, 4431
MhlI GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2827, 3414, 5535
MlsI TGGCCA 2 cut(s) 664, 4251
MluNI TGGCCA 2 cut(s) 664, 4251
MlyI GAGTC 5 cut(s) 49, 2263, 3143, 4492, 4849
MmeI TCCRAC 8 cut(s) 66, 807, 1706, 1924, 3216, 3890, 4133, 5068
Mox20I TGGCCA 2 cut(s) 664, 4251
Mph1103I ATGCAT 3 cut(s) 199, 1720, 1959
MroI TCCGGA 1 cut(s) 3102
MroNI GCCGGC 2 cut(s) 1872, 2250
MroXI GAANNNNTTC 5 cut(s) 696, 863, 2301, 3279, 4080
MscI TGGCCA 2 cut(s) 664, 4251
MslI CAYNNNNRTG 5 cut(s) 2081, 2443, 4257, 4887, 5540
Msp20I TGGCCA 2 cut(s) 664, 4251
MspA1I CMGCKG 3 cut(s) 949, 1682, 5432
MspCI CTTAAG 1 cut(s) 4324
MspI CCGG 7 cut(s) 66, 1016, 1873, 2251, 2551, 3103, 5714
MunI CAATTG 3 cut(s) 2166, 3876, 4814
MvnI CGCG 4 cut(s) 1967, 2739, 5761, 5838
NaeI GCCGGC 2 cut(s) 1874, 2252
NciI CCSGG 1 cut(s) 5714
NcoI CCATGG 1 cut(s) 2986
NdeI CATATG 4 cut(s) 1044, 2998, 3670, 5541
NgoMIV GCCGGC 2 cut(s) 1872, 2250
NheI GCTAGC 3 cut(s) 1069, 2573, 4220
NmeAIII GCCGAG 1 cut(s) 1912
NmuCI GTSAC 5 cut(s) 1708, 2935, 3067, 4375, 4487
NsiI ATGCAT 3 cut(s) 199, 1720, 1959
NspI RCATGY 3 cut(s) 1639, 1837, 2926
NspV TTCGAA 2 cut(s) 2015, 4669
OliI CACNNNNGTG 1 cut(s) 2443
PaeR7I CTCGAG 1 cut(s) 2121
PagI TCATGA 4 cut(s) 600, 619, 2076, 4384
PceI AGGCCT 3 cut(s) 1136, 2050, 3530
PciI ACATGT 1 cut(s) 2922
PcsI WCGNNNNNNNCGW 1 cut(s) 4666
PdiI GCCGGC 2 cut(s) 1874, 2252
PdmI GAANNNNTTC 5 cut(s) 696, 863, 2301, 3279, 4080
PflMI CCANNNNNTGG 1 cut(s) 1118
PfoI TCCNGGA 2 cut(s) 3534, 3934
PleI GAGTC 5 cut(s) 49, 2263, 3142, 4491, 4849
PmaCI CACGTG 1 cut(s) 3998
PmlI CACGTG 1 cut(s) 3998
PpsI GAGTC 5 cut(s) 49, 2263, 3142, 4491, 4849
Ppu21I YACGTR 3 cut(s) 3998, 4364, 5807
PpuMI RGGWCCY 2 cut(s) 2944, 5831
PscI ACATGT 1 cut(s) 2922
PshBI ATTAAT 1 cut(s) 4802
PsiI TTATAA 1 cut(s) 2753
Psp124BI GAGCTC 1 cut(s) 3414
Psp5II RGGWCCY 2 cut(s) 2944, 5831
PspCI CACGTG 1 cut(s) 3998
PspFI CCCAGC 1 cut(s) 1073
PspPPI RGGWCCY 2 cut(s) 2944, 5831
PspXI VCTCGAGB 1 cut(s) 2121
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 2 cut(s) 485, 4480
PsuI RGATCY 7 cut(s) 220, 2400, 2479, 2580, 3099, 3351, 4431
PvuII CAGCTG 2 cut(s) 1682, 5432
RseI CAYNNNNRTG 5 cut(s) 2081, 2443, 4257, 4887, 5540
SacI GAGCTC 1 cut(s) 3414
ScaI AGTACT 3 cut(s) 388, 893, 4654
SchI GAGTC 5 cut(s) 49, 2263, 3143, 4492, 4849
SduI GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2827, 3414, 5535
SexAI ACCWGGT 1 cut(s) 2898
SfcI CTRYAG 6 cut(s) 1173, 1683, 2586, 4495, 4710, 4951
Sfr274I CTCGAG 1 cut(s) 2121
SfuI TTCGAA 2 cut(s) 2015, 4669
SinI GGWCC 7 cut(s) 1778, 2896, 2944, 3907, 3938, 5160, 5831
SlaI CTCGAG 1 cut(s) 2121
SmiMI CAYNNNNRTG 5 cut(s) 2081, 2443, 4257, 4887, 5540
SmlI CTYRAG 3 cut(s) 1003, 2121, 4324
SmoI CTYRAG 3 cut(s) 1003, 2121, 4324
SpeI ACTAGT 2 cut(s) 3797, 4187
SseBI AGGCCT 3 cut(s) 1136, 2050, 3530
SsiI CCGC 9 cut(s) 35, 43, 52, 949, 3445, 3857, 3942, 4632, 5836
SspI AATATT 1 cut(s) 562
SstI GAGCTC 1 cut(s) 3414
StuI AGGCCT 3 cut(s) 1136, 2050, 3530
StyI CCWWGG 3 cut(s) 2325, 2986, 5822
TaiI ACGT 4 cut(s) 277, 4000, 4366, 5809
TaqII GACCGA 1 cut(s) 5177
TatI WGTACW 6 cut(s) 386, 891, 940, 2178, 4652, 4896
TauI GCSGC 1 cut(s) 3860
TscAI CASTG 6 cut(s) 1527, 2632, 3072, 4153, 4485, 4587
TseFI GTSAC 5 cut(s) 1708, 2935, 3067, 4375, 4487
Tsp45I GTSAC 5 cut(s) 1708, 2935, 3067, 4375, 4487
TspGWI ACGGA 3 cut(s) 125, 4766, 4881
TspRI CASTG 6 cut(s) 1527, 2632, 3072, 4153, 4485, 4587
Van91I CCANNNNNTGG 1 cut(s) 1118
Vha464I CTTAAG 1 cut(s) 4324
VneI GTGCAC 2 cut(s) 411, 2084
VpaK11BI GGWCC 7 cut(s) 1778, 2896, 2944, 3907, 3938, 5160, 5831
VspI ATTAAT 1 cut(s) 4802
XagI CCTNNNNNAGG 1 cut(s) 1648
XbaI TCTAGA 2 cut(s) 277, 2272
XceI RCATGY 3 cut(s) 1639, 1837, 2926
XcmI CCANNNNNNNNNTGG 1 cut(s) 3298
XhoI CTCGAG 1 cut(s) 2121
XmaJI CCTAGG 1 cut(s) 5822
XmiI GTMKAC 3 cut(s) 1985, 3834, 5316
XmnI GAANNNNTTC 5 cut(s) 696, 863, 2301, 3279, 4080
ZrmI AGTACT 3 cut(s) 388, 893, 4654
Zsp2I ATGCAT 3 cut(s) 199, 1720, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.