RchiOBHm_Chr1g0313331

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002059
Physical Location & Seq
Forward (+)
432018 .. 435098
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54415

Sequence Viewer

Length: 471 bp
ATGTTTGGTTTTCAGAAGGATAATGTTGCTTGTCAAAGGGAGCGCTTGATTCTTTTGATCGCTAGTGTTCACATATGGCAATTACCAAAATCTGATCAGCAGCCCAAGGTTGCCAACTATTCAGCAGGAAGTACAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATATGGGGAGAGGCTGCCAACTTGAGATTCATGCCGGAATGCCTTTGCTACATTTATCATCATATTGGAGGGCATATGGTATGGCATTTGAATTATATGGAATGCTAACTGGGAGTGTTAGTCCAATTACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGGGGCCTTTTCGGGGAAAGTAGTAACTCCAATATATAATACTATACCAAAGGAGGCTGAAAGGAGCAAAAGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACTTGAATGAATACTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

156

Amino Acids

17.56

Weight (kDa)

8.51

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 85 - 156 1.4e-15 1,3-beta-glucan synthase subunit FKS1, domain-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 326
AciI CCGC 1 cut(s) 140
AfaI GTAC 1 cut(s) 133
AfeI AGCGCT 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 302, 326, 351
AgsI TTSAA 2 cut(s) 263, 454
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
Alw21I GWGCWC 1 cut(s) 312
Aor51HI AGCGCT 1 cut(s) 44
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 100, 137, 186
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 342
AsuHPI GGTGA 2 cut(s) 317, 344
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 112, 149, 173
BclI TGATCA 1 cut(s) 94
BfaI CTAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 46
BisI GCNGC 3 cut(s) 101, 138, 187
BlsI GCNGC 3 cut(s) 102, 139, 188
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 343
BmrI ACTGGG 1 cut(s) 291
BmuI ACTGGG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 277, 307
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 326, 351
Bse1I ACTGG 1 cut(s) 286
BseDI CCNNGG 1 cut(s) 105
BseLI CCNNNNNNNGG 3 cut(s) 302, 326, 351
BseNI ACTGG 1 cut(s) 286
BseXI GCAGC 3 cut(s) 112, 149, 173
BshFI GGCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 3 cut(s) 302, 326, 351
BsmI GAATGC 3 cut(s) 216, 279, 424
BsnI GGCC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 57, 94
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 344
BspLI GGNNCC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 2 cut(s) 57, 94
BssT1I CCWWGG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 2 cut(s) 60, 97
BstMBI GATC 2 cut(s) 57, 94
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstV1I GCAGC 3 cut(s) 112, 149, 173
BsuRI GGCC 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 132
CviAII CATG 2 cut(s) 155, 203
CviJI RGCY 6 cut(s) 103, 186, 319, 323, 344, 395
CviKI_1 RGCY 6 cut(s) 103, 186, 319, 323, 344, 395
CviQI GTAC 1 cut(s) 132
DpnI GATC 2 cut(s) 59, 96
DpnII GATC 2 cut(s) 57, 94
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 105
Eco47III AGCGCT 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 2 cut(s) 158, 206
FatI CATG 2 cut(s) 154, 202
FauNDI CATATG 2 cut(s) 74, 247
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 101, 138, 187
Fsp4HI GCNGC 3 cut(s) 101, 138, 187
FspBI CTAG 1 cut(s) 63
GlaI GCGC 1 cut(s) 44
GluI GCNGC 3 cut(s) 101, 138, 187
HaeII RGCGCY 1 cut(s) 46
HaeIII GGCC 1 cut(s) 344
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 45
Hin1II CATG 2 cut(s) 158, 206
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 2 cut(s) 49, 199
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 317, 344
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 15, 94
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 10
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
Hsp92II CATG 2 cut(s) 158, 206
HspAI GCGC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 57, 94
LmnI GCTCC 2 cut(s) 40, 402
LpnPI CCDG 4 cut(s) 111, 220, 267, 333
Lsp1109I GCAGC 3 cut(s) 112, 149, 173
MaeI CTAG 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 361
MalI GATC 2 cut(s) 59, 96
MboI GATC 2 cut(s) 57, 94
MboII GAAGA 2 cut(s) 159, 347
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 5 cut(s) 80, 144, 263, 297, 439
MnlI CCTC 5 cut(s) 176, 234, 331, 385, 404
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 207
Mva1269I GAATGC 3 cut(s) 216, 279, 424
MwoI GCNNNNNNNGC 1 cut(s) 316
NdeI CATATG 2 cut(s) 74, 247
NdeII GATC 2 cut(s) 57, 94
NlaIII CATG 2 cut(s) 158, 206
NlaIV GGNNCC 1 cut(s) 343
PctI GAATGC 3 cut(s) 216, 279, 424
PfeI GAWTC 2 cut(s) 49, 199
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 3 cut(s) 102, 139, 188
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 1 cut(s) 342
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SatI GCNGC 3 cut(s) 101, 138, 187
Sau3AI GATC 2 cut(s) 57, 94
Sau96I GGNCC 1 cut(s) 342
SduI GDGCHC 1 cut(s) 312
SetI ASST 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 5 cut(s) 80, 144, 263, 297, 439
SsiI CCGC 1 cut(s) 140
SspMI CTAG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 105
TasI AATT 5 cut(s) 80, 144, 263, 297, 439
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 2 cut(s) 49, 199
TseI GCWGC 3 cut(s) 100, 137, 186
TspDTI ATGAA 2 cut(s) 191, 471
Van91I CCANNNNNTGG 1 cut(s) 326
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.