FvH4_7g00110

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002059
Physical Location & Seq
Forward (+)
687633 .. 706247
18615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00110.t1

Sequence Viewer

Length: 5850 bp
ATGGCATACAGAAGGGGGTTCGACCAGCAGCAGCCGCAGAGGCGGATTCTGCGGACTCAGACTGCTGGAAGTTTTGGAGAGCCTATGTTGGATAGTGAGGTCGTGCCTTCCTCTCTGGTGGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAACGCACGAGTTGCCTATCTCTGTCGGTTCTATGCATTTGAGAAAGCTCACAGATTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAACGTCTTGAAAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGCTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCAAAATGCTGCTGATAAAGCTGATCGTGCACAACTGACAAAGGCATACCAAACTGCTGCTGTTTTATTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTTGCTGAGGAGATTTTGGAAGCTCACACTAAGGTTGAAGAAAAGCAACAGATATATCTTCCTTACAATATTCTTCCTCTTGATCCTGATAGTAAAAATCAGGCAATCATGAGCTATCCCGAGATTATTGCTACTGTCTATGCACTCCGAAACACCAGGGGTCTACCCTGGCCGAAGGACTACAAAAAGAAAGTTGATGAAGATATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAATGTCGCCAATCAAAGGGAGCACTTGATTCTCTTGGTTGCTAATGTTCACGTACGGCAATTACCAAAACCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACGATAGTGATGAAGAAACTTTTCAAAAATTACAAAAAGTGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACCATTCAGCAGGAAGTGCAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATATGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTCTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTCGCTGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGGAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACTTGAATGAATACTTTTGGTCAGTTGATTGTTTCAAGCTAGGCTGGCCTATGCGTGCTGATGCCAATTTCTTTTACCAGCCTAGTAAAGAGCCTGAACTTGACAAAGAGAAGAAAAACAAACCATACACTGGGCATCGATGGATTGGCAAAGTCAACTTTGTCGAGATTCGTTCATTTTGGCACATTTTCAGAAGCTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGAAATTAAGTTCAGTCTTCGAGGGTGATGTCTTCAAGAAAGTGTTGAGTATCTTCATAACTGCTGCAATGTTAAAGCTTGCACAAGCTGTTCTTGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGTGTTTGTCAGGCTAAGATATGTCCTCAAGGCTGTTTCAGCTGCAGCTTGGGTCATTGTTTTACCCGTGACTTATGCATATAGTTGGAAAAACCCTGGGGGATTTGCACAGACCATCAGGGGTTGGTTTGGCAATGGTCCAACCTCATCTTCTTTGTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGTATCTTGAAAGATCAAACTTTAAGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCAAAATTATCCTTCAGTTACTTTGTTGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATCTCGACCTATGAATGGCATGAGTTCTTCCCTCGAGCCAAGAATAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTCGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGACTAAGAAGAAGGGACTGAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCATCCAATAAAGAGAAGGAGGCAGCAAAATTTGCACAGATGTGGAATGAAATAATCAGTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCGGACCCAGATTTGAACATCATCCAGTGGCCTCCTTTCCTCCTTGCTAGCAAGATCCCTATAGCACTGGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGAGAAGGACAATTATATGTGCTGTGCTGTTAGAGAGTGCTATCTTTCGTTTAGAAGTATTATCAATGTTTTAGTCCTTGGAGAACGCGAAAAGATGGTTATAAATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACACTGACGAAGGAAGTTAAATTGAGTGCTCTCCCCAGCCTCCACGAACAATTTGTTAAACTAATTGAATATCTGTTGGACAACAAAAAGGAGGACAAGGATCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGATGATGAGATTCCCAGCTTGCTAGATTCAAACCATGGCGGATCATATGGAAAAGATGAGGGAATGAGACCCCTTGATCAACGAGATACATACTTTGGCTCACTAAAATTTCCAGTGACTCCACTAACAGAGGCTTGGAAAGAGAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATGTCTTTTTTTTCCAATTCGTTGTTTATGGACATGCCTCCTGCACCCAAAGTTCGAAATATGCTTTCGTTCTCGGTTTTGACACCCTACCATTCAGAAGAAGTTCTTTTCTCCATAAAACACCTGGAACAGGAGAACGATGATGGTGTCTCTATTCTTTTCTACCTGCAAAAGATATTTCCAGATGAATGGACTAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAGCTCAGAGCAAATGAAGACTTGGAGGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTAGATATGGCAAAAGATGAAGATCTAATGGAAGGGTACAAGGCTGCTGAATCAACGATAGAGGAACATCATTCAAAGGCTGAAAGGTCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTCATAAGCGATCCGGAAATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAGAAGATGGTTAGGAAGGTCTACTATTCTGCTCTTGTAAAGGCAGCTCCTCCCACCAAGTCAATTGATTCATCTGATCCAGTGCAGAGATTAGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAATCATGCCATTATTTTCACACGTGGAGAAGGATTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAGGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAGAAAGCATGATGGTAGACCTCCAACAATACTTGGGCTTAGGGAGCATATATTTACGGGCAGTGTATCGTCTCTTGCATGGTTTATGTCTAATCAGGAGACCAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTATTTCATCTGACCAGAGGGGGTGTGAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAACTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGTGATGTCGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAATGGTGAGCAGACACTGAGCCGTGACATCTACAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAATTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTATATGTGTTTCTCTACGGTCGCCTTTATCTGGTTATGAGCGGACTTGAAAAAGGGCTTAGTACTCAACGAGCTATTCGAGACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTGATGGCCTTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTCATCTTAATGCAATTACAACTGGCCCCTGTATTTTTCACATTTTCACTTGGTACTAAGACCCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAATTACAGGCTCTACTCCCGTAGCCATTTTGTCAAGGGTATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACCGTACAAATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTGTTCAACCCATCAGGTTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAATGGATAAGTAATCATGGAGGGATCGGTGTATCCCCTGAAAAAAGTTGGGAATCCTGGTGGGAAAAGGAACATGAGCATCTTCGCTACTCTGGGATGCGCGGTATCATTACCGAGATATTATTAGCATTGCGCTTTTTCGTCTACCAGTTTGGGCTTGTATATCACCTCAACATTACAAAGGACAAAAGTATCCTGGTCTATGGTGTTTCGTGGCTGGTGATCATCGGTGTTTTGTCGCTGATGAAGGCTGTGTCTGCTGGAAGGCGAAGATTAAGTGCAGACTTTCAGCTTTTGTTTCGACTTGTTAAGGGATGTATGTTTATTACACTCTTATCGGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGCTAAAGGATGTTGTAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTCTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCAGTCGATTCAAACACTTGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCCTGGTTCCCTTTTGTTTCCGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTCGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCGAGGAAGGGTCCCAAGGCTAGCAATAGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1950

Amino Acids

225.02

Weight (kDa)

9.29

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 169 5.3e-17 Vta1 like
FKS1_dom1 PF14288 318 - 430 8.8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 852 - 1041 4.2e-62 Callose synthase 1 domain
Glucan_synthase PF02364 1049 - 1139 6.4e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1143 - 1760 1.2e-207 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2750
Acc36I ACCTGC 2 cut(s) 3348, 4328
AccB7I CCANNNNNTGG 2 cut(s) 1118, 1370
AccBSI CCGCTC 1 cut(s) 4626
AccI GTMKAC 5 cut(s) 655, 1985, 3828, 4096, 5310
AccII CGCG 4 cut(s) 1967, 2736, 5268, 5755
AccIII TCCGGA 1 cut(s) 3704
AcoI YGGCCR 3 cut(s) 238, 662, 4243
AcuI CTGAAG 4 cut(s) 695, 2011, 3420, 3506
AcvI CACGTG 1 cut(s) 3992
AdeI CACNNNGTG 1 cut(s) 4784
AfeI AGCGCT 1 cut(s) 3513
AflII CTTAAG 1 cut(s) 4318
AflIII ACRYGT 2 cut(s) 2919, 3989
AhdI GACNNNNNGTC 1 cut(s) 4409
AhlI ACTAGT 1 cut(s) 3791
AjiI CACGTC 1 cut(s) 4406
AjuI GAANNNNNNNTTGG 4 cut(s) 992, 1024, 1774, 1806
AleI CACNNNNGTG 1 cut(s) 2440
AloI GAACNNNNNNTCC 4 cut(s) 1501, 1533, 4751, 4783
Alw21I GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2824, 3411, 5529
Alw26I GTCTC 7 cut(s) 1929, 2342, 3010, 3328, 4155, 4172, 4659
Alw44I GTGCAC 2 cut(s) 411, 2084
AlwNI CAGNNNCTG 2 cut(s) 485, 4474
Ama87I CYCGRG 3 cut(s) 611, 2121, 2273
Aor13HI TCCGGA 1 cut(s) 3704
Aor51HI AGCGCT 1 cut(s) 3513
ApaLI GTGCAC 2 cut(s) 411, 2084
Asp700I GAANNNNTTC 4 cut(s) 696, 863, 3276, 4074
AspLEI GCGC 4 cut(s) 1967, 3514, 5268, 5301
AspS9I GGNCC 8 cut(s) 1778, 2527, 2941, 3932, 4687, 4813, 5813, 5825
AsuC2I CCSGG 1 cut(s) 5708
AsuHPI GGTGA 7 cut(s) 1109, 1136, 1544, 4475, 4531, 5324, 5398
AsuII TTCGAA 1 cut(s) 3229
AsuNHI GCTAGC 3 cut(s) 2570, 4214, 5834
AvaI CYCGRG 3 cut(s) 611, 2121, 2273
AvaII GGWCC 5 cut(s) 1778, 2527, 2941, 3932, 5825
AxyI CCTNAGG 1 cut(s) 2334
BaeGI GKGCMC 2 cut(s) 415, 2088
BaeI ACNNNNGTAYC 2 cut(s) 5341, 5374
BalI TGGCCA 1 cut(s) 4245
BanII GRGCYC 2 cut(s) 2164, 3411
BarI GAAGNNNNNNTAC 2 cut(s) 2195, 2227
BauI CACGAG 4 cut(s) 171, 330, 3714, 5667
BbrPI CACGTG 1 cut(s) 3992
BbsI GAAGAC 4 cut(s) 1516, 1531, 3429, 3804
Bbv12I GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2824, 3411, 5529
BbvCI CCTCAGC 1 cut(s) 498
BceAI ACGGC 3 cut(s) 225, 809, 4464
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciVI GTATCC 3 cut(s) 2182, 5209, 5369
BclI TGATCA 6 cut(s) 811, 1482, 2776, 3025, 4311, 5388
BcnI CCSGG 1 cut(s) 5708
BcoDI GTCTC 7 cut(s) 1929, 2342, 3010, 3328, 4155, 4172, 4659
BcuI ACTAGT 1 cut(s) 3791
BfmI CTRYAG 5 cut(s) 1173, 1683, 2583, 4489, 4704
BfoI RGCGCY 1 cut(s) 3515
BfrI CTTAAG 1 cut(s) 4318
BfuAI ACCTGC 2 cut(s) 3348, 4328
BfuI GTATCC 3 cut(s) 2182, 5209, 5369
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 2 cut(s) 3553, 4425
BmcAI AGTACT 2 cut(s) 893, 4648
Bme18I GGWCC 5 cut(s) 1778, 2527, 2941, 3932, 5825
BmeRI GACNNNNNGTC 1 cut(s) 4409
BmeT110I CYCGRG 3 cut(s) 611, 2121, 2273
BmgBI CACGTC 1 cut(s) 4406
BmgT120I GGNCC 8 cut(s) 1778, 2527, 2941, 3932, 4687, 4813, 5813, 5825
BmiI GGNNCC 9 cut(s) 2163, 2529, 2942, 3934, 4769, 4815, 5729, 5826, 5827
BmrI ACTGGG 1 cut(s) 1380
BmsI GCATC 7 cut(s) 316, 1291, 1384, 1896, 3461, 5253, 5254
BmtI GCTAGC 3 cut(s) 2574, 4218, 5838
BmuI ACTGGG 1 cut(s) 1380
BpiI GAAGAC 4 cut(s) 1516, 1531, 3429, 3804
BplI GAGNNNNNCTC 2 cut(s) 313, 345
Bpu10I CCTNAGC 2 cut(s) 498, 4118
Bpu14I TTCGAA 1 cut(s) 3229
BpuEI CTTGAG 2 cut(s) 1024, 1652
BpuMI CCSGG 1 cut(s) 5708
Bsa29I ATCGAT 1 cut(s) 1378
BsaAI YACGTR 4 cut(s) 790, 3992, 4358, 5801
BsaBI GATNNNNATC 3 cut(s) 2296, 3552, 3912
BsaI GGTCTC 3 cut(s) 1929, 3010, 4172
BsaWI WCCGGW 2 cut(s) 3504, 3704
BsaXI ACNNNNNCTCC 6 cut(s) 1069, 1099, 3116, 3146, 4412, 4442
Bse118I RCCGGY 2 cut(s) 1872, 2250
Bse21I CCTNAGG 1 cut(s) 2334
Bse3DI GCAATG 4 cut(s) 1485, 1581, 1780, 5294
Bse8I GATNNNNATC 3 cut(s) 2296, 3552, 3912
BseAI TCCGGA 1 cut(s) 3704
BseCI ATCGAT 1 cut(s) 1378
BseJI GATNNNNATC 3 cut(s) 2296, 3552, 3912
BseMI GCAATG 4 cut(s) 1485, 1581, 1780, 5294
BseRI GAGGAG 5 cut(s) 515, 1145, 2351, 3446, 3846
BseSI GKGCMC 2 cut(s) 415, 2088
BseYI CCCAGC 3 cut(s) 1073, 2828, 2963
BsgI GTGCAG 7 cut(s) 249, 962, 3201, 3911, 4761, 4905, 5466
Bsh1236I CGCG 4 cut(s) 1967, 2736, 5268, 5755
Bsh1285I CGRYCG 1 cut(s) 4606
BshVI ATCGAT 1 cut(s) 1378
BsiEI CGRYCG 1 cut(s) 4606
BsiHKAI GWGCWC 7 cut(s) 415, 762, 1104, 2088, 2824, 3411, 5529
BsiHKCI CYCGRG 3 cut(s) 611, 2121, 2273
BsiSI CCGG 5 cut(s) 1873, 2251, 3505, 3705, 5708
BsiWI CGTACG 1 cut(s) 790
BslFI GGGAC 4 cut(s) 2376, 2498, 2954, 5811
BsmAI GTCTC 7 cut(s) 1929, 2342, 3010, 3328, 4155, 4172, 4659
BsmBI CGTCTC 1 cut(s) 4155
BsmFI GGGAC 4 cut(s) 2376, 2498, 2954, 5811
Bso31I GGTCTC 3 cut(s) 1929, 3010, 4172
BsoBI CYCGRG 3 cut(s) 611, 2121, 2273
Bsp119I TTCGAA 1 cut(s) 3229
Bsp1286I GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2824, 3411, 5529
Bsp13I TCCGGA 1 cut(s) 3704
Bsp19I CCATGG 1 cut(s) 2983
BspDI ATCGAT 1 cut(s) 1378
BspEI TCCGGA 1 cut(s) 3704
BspFNI CGCG 4 cut(s) 1967, 2736, 5268, 5755
BspHI TCATGA 3 cut(s) 600, 2076, 4378
BspLI GGNNCC 9 cut(s) 2163, 2529, 2942, 3934, 4769, 4815, 5729, 5826, 5827
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 2 cut(s) 3348, 4328
BspOI GCTAGC 3 cut(s) 2574, 4218, 5838
BspT104I TTCGAA 1 cut(s) 3229
BspTI CTTAAG 1 cut(s) 4318
BspTNI GGTCTC 3 cut(s) 1929, 3010, 4172
BsrBI CCGCTC 1 cut(s) 4626
BsrDI GCAATG 4 cut(s) 1485, 1581, 1780, 5294
BsrFI RCCGGY 2 cut(s) 1872, 2250
BssAI RCCGGY 2 cut(s) 1872, 2250
BssNAI GTATAC 1 cut(s) 1986
BssSI CACGAG 4 cut(s) 171, 330, 3714, 5667
BssT1I CCWWGG 3 cut(s) 2725, 2983, 5829
Bst1107I GTATAC 1 cut(s) 1986
Bst2BI CACGAG 4 cut(s) 171, 330, 3714, 5667
Bst4CI ACNGT 8 cut(s) 628, 2624, 3484, 3684, 4576, 4585, 4604, 5062
Bst6I CTCTTC 6 cut(s) 1122, 1942, 2466, 3762, 4035, 4683
BstAFI CTTAAG 1 cut(s) 4318
BstAPI GCANNNNNTGC 3 cut(s) 176, 940, 2432
BstBAI YACGTR 4 cut(s) 790, 3992, 4358, 5801
BstBI TTCGAA 1 cut(s) 3229
BstDSI CCRYGG 2 cut(s) 241, 2983
BstENI CCTNNNNNAGG 1 cut(s) 3302
BstFNI CGCG 4 cut(s) 1967, 2736, 5268, 5755
BstH2I RGCGCY 1 cut(s) 3515
BstHHI GCGC 4 cut(s) 1967, 3514, 5268, 5301
BstMAI GTCTC 7 cut(s) 1929, 2342, 3010, 3328, 4155, 4172, 4659
BstMCI CGRYCG 1 cut(s) 4606
BstNSI RCATGY 4 cut(s) 1639, 1837, 2923, 3211
BstSFI CTRYAG 5 cut(s) 1173, 1683, 2583, 4489, 4704
BstSLI GKGCMC 2 cut(s) 415, 2088
BstUI CGCG 4 cut(s) 1967, 2736, 5268, 5755
BstV2I GAAGAC 4 cut(s) 1516, 1531, 3429, 3804
BstX2I RGATCY 7 cut(s) 223, 2397, 2476, 2577, 3096, 3553, 4425
BstXI CCANNNNNNTGG 5 cut(s) 3363, 3686, 3814, 5018, 5586
BstYI RGATCY 7 cut(s) 223, 2397, 2476, 2577, 3096, 3553, 4425
BstZ17I GTATAC 1 cut(s) 1986
Bsu15I ATCGAT 1 cut(s) 1378
Bsu36I CCTNAGG 1 cut(s) 2334
BsuI GTATCC 3 cut(s) 2182, 5209, 5369
BsuTUI ATCGAT 1 cut(s) 1378
BtgI CCRYGG 2 cut(s) 241, 2983
BtrI CACGTC 1 cut(s) 4406
BtsI GCAGTG 2 cut(s) 2300, 4147
BveI ACCTGC 2 cut(s) 3348, 4328
CaiI CAGNNNCTG 2 cut(s) 485, 4474
CciI TCATGA 3 cut(s) 600, 2076, 4378
CfoI GCGC 4 cut(s) 1967, 3514, 5268, 5301
Cfr10I RCCGGY 2 cut(s) 1872, 2250
Cfr13I GGNCC 8 cut(s) 1778, 2527, 2941, 3932, 4687, 4813, 5813, 5825
ClaI ATCGAT 1 cut(s) 1378
CseI GACGC 1 cut(s) 5548
DraI TTTAAA 1 cut(s) 259
DraIII CACNNNGTG 1 cut(s) 4784
DriI GACNNNNNGTC 1 cut(s) 4409
EaeI YGGCCR 3 cut(s) 238, 662, 4243
Eam1104I CTCTTC 6 cut(s) 1122, 1942, 2466, 3762, 4035, 4683
Eam1105I GACNNNNNGTC 1 cut(s) 4409
EarI CTCTTC 6 cut(s) 1122, 1942, 2466, 3762, 4035, 4683
EciI GGCGGA 4 cut(s) 58, 2540, 3003, 3431
Ecl136II GAGCTC 1 cut(s) 3409
Eco130I CCWWGG 3 cut(s) 2725, 2983, 5829
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3527
Eco24I GRGCYC 2 cut(s) 2164, 3411
Eco31I GGTCTC 3 cut(s) 1929, 3010, 4172
Eco32I GATATC 1 cut(s) 2074
Eco47I GGWCC 5 cut(s) 1778, 2527, 2941, 3932, 5825
Eco47III AGCGCT 1 cut(s) 3513
Eco53kI GAGCTC 1 cut(s) 3409
Eco57I CTGAAG 4 cut(s) 695, 2011, 3420, 3506
Eco72I CACGTG 1 cut(s) 3992
Eco81I CCTNAGG 1 cut(s) 2334
Eco88I CYCGRG 3 cut(s) 611, 2121, 2273
EcoICRI GAGCTC 1 cut(s) 3409
EcoNI CCTNNNNNAGG 1 cut(s) 3302
EcoO109I RGGNCCY 2 cut(s) 2941, 5825
EcoRI GAATTC 2 cut(s) 3690, 5744
EcoRV GATATC 1 cut(s) 2074
EcoT14I CCWWGG 3 cut(s) 2725, 2983, 5829
EcoT22I ATGCAT 3 cut(s) 202, 1720, 1959
EcoT38I GRGCYC 2 cut(s) 2164, 3411
ErhI CCWWGG 3 cut(s) 2725, 2983, 5829
Esp3I CGTCTC 1 cut(s) 4155
FalI AAGNNNNNCTT 8 cut(s) 684, 716, 1584, 1616, 3264, 3296, 4674, 4706
FaqI GGGAC 4 cut(s) 2376, 2498, 2954, 5811
FauI CCCGC 1 cut(s) 3943
FauNDI CATATG 4 cut(s) 1044, 2995, 3664, 5535
FbaI TGATCA 6 cut(s) 811, 1482, 2776, 3025, 4311, 5388
FblI GTMKAC 5 cut(s) 655, 1985, 3828, 4096, 5310
FriOI GRGCYC 2 cut(s) 2164, 3411
GlaI GCGC 4 cut(s) 1966, 3513, 5267, 5300
GsaI CCCAGC 3 cut(s) 1077, 2832, 2967
HaeII RGCGCY 1 cut(s) 3515
HapII CCGG 5 cut(s) 1873, 2251, 3505, 3705, 5708
HgaI GACGC 1 cut(s) 5548
HhaI GCGC 4 cut(s) 1967, 3514, 5268, 5301
Hin6I GCGC 4 cut(s) 1965, 3512, 5266, 5299
HinP1I GCGC 4 cut(s) 1965, 3512, 5266, 5299
HincII GTYRAC 3 cut(s) 1396, 3649, 4572
HindII GTYRAC 3 cut(s) 1396, 3649, 4572
HindIII AAGCTT 6 cut(s) 378, 1435, 1583, 3923, 4299, 5610
HpaI GTTAAC 1 cut(s) 4572
HpaII CCGG 5 cut(s) 1873, 2251, 3505, 3705, 5708
HphI GGTGA 7 cut(s) 1109, 1136, 1544, 4475, 4531, 5324, 5398
HpyCH4III ACNGT 8 cut(s) 628, 2624, 3484, 3684, 4576, 4585, 4604, 5062
HpyCH4IV ACGT 6 cut(s) 277, 789, 3991, 4357, 4405, 5800
HpySE526I ACGT 6 cut(s) 277, 789, 3991, 4357, 4405, 5800
HspAI GCGC 4 cut(s) 1965, 3512, 5266, 5299
KflI GGGWCCC 1 cut(s) 5825
Kpn2I TCCGGA 1 cut(s) 3704
KroI GCCGGC 1 cut(s) 2250
KroNI GCCGGC 1 cut(s) 2252
Ksp22I TGATCA 6 cut(s) 811, 1482, 2776, 3025, 4311, 5388
KspAI GTTAAC 1 cut(s) 4572
LmnI GCTCC 8 cut(s) 133, 757, 1194, 1452, 2167, 3859, 4123, 5104
LweI GCATC 7 cut(s) 316, 1291, 1384, 1896, 3461, 5253, 5254
MaeII ACGT 6 cut(s) 277, 789, 3991, 4357, 4405, 5800
MaeIII GTNAC 8 cut(s) 1153, 1708, 2030, 2932, 3064, 4189, 4369, 4481
MbiI CCGCTC 1 cut(s) 4626
MfeI CAATTG 3 cut(s) 2166, 3870, 4545
MflI RGATCY 7 cut(s) 223, 2397, 2476, 2577, 3096, 3553, 4425
MhlI GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2824, 3411, 5529
MlsI TGGCCA 1 cut(s) 4245
MluNI TGGCCA 1 cut(s) 4245
MlyI GAGTC 4 cut(s) 49, 2263, 3061, 3140
MmeI TCCRAC 8 cut(s) 69, 807, 1706, 1805, 1924, 2850, 4127, 5062
Mox20I TGGCCA 1 cut(s) 4245
Mph1103I ATGCAT 3 cut(s) 202, 1720, 1959
MroI TCCGGA 1 cut(s) 3704
MroNI GCCGGC 1 cut(s) 2250
MroXI GAANNNNTTC 4 cut(s) 696, 863, 3276, 4074
MscI TGGCCA 1 cut(s) 4245
MslI CAYNNNNRTG 7 cut(s) 1640, 2081, 2440, 4251, 4796, 4881, 5534
Msp20I TGGCCA 1 cut(s) 4245
MspA1I CMGCKG 2 cut(s) 949, 1682
MspCI CTTAAG 1 cut(s) 4318
MspI CCGG 5 cut(s) 1873, 2251, 3505, 3705, 5708
MunI CAATTG 3 cut(s) 2166, 3870, 4545
MvnI CGCG 4 cut(s) 1967, 2736, 5268, 5755
NaeI GCCGGC 1 cut(s) 2252
NciI CCSGG 1 cut(s) 5708
NcoI CCATGG 1 cut(s) 2983
NdeI CATATG 4 cut(s) 1044, 2995, 3664, 5535
NgoMIV GCCGGC 1 cut(s) 2250
NheI GCTAGC 3 cut(s) 2570, 4214, 5834
NlaIV GGNNCC 9 cut(s) 2163, 2529, 2942, 3934, 4769, 4815, 5729, 5826, 5827
NmeAIII GCCGAG 2 cut(s) 1912, 5841
NmuCI GTSAC 5 cut(s) 1708, 2932, 3064, 4369, 4481
NsiI ATGCAT 3 cut(s) 202, 1720, 1959
NspI RCATGY 4 cut(s) 1639, 1837, 2923, 3211
NspV TTCGAA 1 cut(s) 3229
OliI CACNNNNGTG 1 cut(s) 2440
PaeR7I CTCGAG 2 cut(s) 2121, 2273
PagI TCATGA 3 cut(s) 600, 2076, 4378
PasI CCCWGGG 1 cut(s) 1736
PceI AGGCCT 3 cut(s) 1136, 2050, 3527
PciI ACATGT 1 cut(s) 2919
PcsI WCGNNNNNNNCGW 1 cut(s) 4660
PdiI GCCGGC 1 cut(s) 2252
PdmI GAANNNNTTC 4 cut(s) 696, 863, 3276, 4074
Pfl23II CGTACG 1 cut(s) 790
PflMI CCANNNNNTGG 2 cut(s) 1118, 1370
PfoI TCCNGGA 1 cut(s) 3928
PleI GAGTC 4 cut(s) 49, 2263, 3061, 3139
PmaCI CACGTG 1 cut(s) 3992
PmlI CACGTG 1 cut(s) 3992
PpsI GAGTC 4 cut(s) 49, 2263, 3061, 3139
Ppu21I YACGTR 4 cut(s) 790, 3992, 4358, 5801
PpuMI RGGWCCY 2 cut(s) 2941, 5825
PscI ACATGT 1 cut(s) 2919
PsiI TTATAA 1 cut(s) 2750
Psp124BI GAGCTC 1 cut(s) 3411
Psp5II RGGWCCY 2 cut(s) 2941, 5825
PspCI CACGTG 1 cut(s) 3992
PspFI CCCAGC 3 cut(s) 1073, 2828, 2963
PspLI CGTACG 1 cut(s) 790
PspN4I GGNNCC 9 cut(s) 2163, 2529, 2942, 3934, 4769, 4815, 5729, 5826, 5827
PspPI GGNCC 8 cut(s) 1778, 2527, 2941, 3932, 4687, 4813, 5813, 5825
PspPPI RGGWCCY 2 cut(s) 2941, 5825
PspXI VCTCGAGB 1 cut(s) 2121
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 2 cut(s) 485, 4474
PsuI RGATCY 7 cut(s) 223, 2397, 2476, 2577, 3096, 3553, 4425
PvuII CAGCTG 1 cut(s) 1682
RseI CAYNNNNRTG 7 cut(s) 1640, 2081, 2440, 4251, 4796, 4881, 5534
SacI GAGCTC 1 cut(s) 3411
Sau96I GGNCC 8 cut(s) 1778, 2527, 2941, 3932, 4687, 4813, 5813, 5825
ScaI AGTACT 2 cut(s) 893, 4648
SchI GAGTC 4 cut(s) 49, 2263, 3061, 3140
SduI GDGCHC 8 cut(s) 415, 762, 1104, 2088, 2164, 2824, 3411, 5529
SfaNI GCATC 7 cut(s) 316, 1291, 1384, 1896, 3461, 5253, 5254
SfcI CTRYAG 5 cut(s) 1173, 1683, 2583, 4489, 4704
Sfr274I CTCGAG 2 cut(s) 2121, 2273
SfuI TTCGAA 1 cut(s) 3229
SinI GGWCC 5 cut(s) 1778, 2527, 2941, 3932, 5825
SlaI CTCGAG 2 cut(s) 2121, 2273
SmiMI CAYNNNNRTG 7 cut(s) 1640, 2081, 2440, 4251, 4796, 4881, 5534
SmlI CTYRAG 5 cut(s) 1003, 1667, 2121, 2273, 4318
SmoI CTYRAG 5 cut(s) 1003, 1667, 2121, 2273, 4318
SpeI ACTAGT 1 cut(s) 3791
SseBI AGGCCT 3 cut(s) 1136, 2050, 3527
SspI AATATT 1 cut(s) 562
SstI GAGCTC 1 cut(s) 3411
StuI AGGCCT 3 cut(s) 1136, 2050, 3527
StyI CCWWGG 3 cut(s) 2725, 2983, 5829
TaaI ACNGT 8 cut(s) 628, 2624, 3484, 3684, 4576, 4585, 4604, 5062
TaiI ACGT 6 cut(s) 280, 792, 3994, 4360, 4408, 5803
TaqII GACCGA 1 cut(s) 5494
TatI WGTACW 4 cut(s) 891, 2178, 4646, 4890
TauI GCSGC 1 cut(s) 37
TseFI GTSAC 5 cut(s) 1708, 2932, 3064, 4369, 4481
Tsp45I GTSAC 5 cut(s) 1708, 2932, 3064, 4369, 4481
TspGWI ACGGA 4 cut(s) 128, 3137, 4760, 4875
Van91I CCANNNNNTGG 2 cut(s) 1118, 1370
Vha464I CTTAAG 1 cut(s) 4318
VneI GTGCAC 2 cut(s) 411, 2084
VpaK11BI GGWCC 5 cut(s) 1778, 2527, 2941, 3932, 5825
XagI CCTNNNNNAGG 1 cut(s) 3302
XceI RCATGY 4 cut(s) 1639, 1837, 2923, 3211
XcmI CCANNNNNNNNNTGG 1 cut(s) 3295
XhoI CTCGAG 2 cut(s) 2121, 2273
XmiI GTMKAC 5 cut(s) 655, 1985, 3828, 4096, 5310
XmnI GAANNNNTTC 4 cut(s) 696, 863, 3276, 4074
ZrmI AGTACT 2 cut(s) 893, 4648
Zsp2I ATGCAT 3 cut(s) 202, 1720, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.