MD02G1319100.v1.1

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002059
Physical Location & Seq
Reverse (-)
37346607 .. 37365311
18705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1319100.v1.1.491

Sequence Viewer

Length: 5808 bp
ATGGCGTATAGAAGAGGAGGGTTTGACGGCCAGCCGCCGAGGCGGATTCTGCGCACCCAGACTGCTGGGAGCTTTGGAGAGACCATGCTGGATAGTGAGGTGGTGCCTTCTTCCCTCTTCGAGATTGCTCCTATTCTCCGTGTTGCAAACGAAGTTGAGTCCAGGAATCCAAGAGTTGCCTATCTATGTCGGTTCTATGCCTTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGACGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAGCGTCTAGAAAGGGAAAATGAAACAACACTGGCAGGAAGGGCAAAGAGTGATGCTCGGGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGCAGCATGCTACTGATAAAGCAGATCGGGCACAACTTACAAAAGCATACCAAACTGCTGCTGTTTTATTTGAGGTTTTGAAGGCTGTCAATCAGACGGAGGCTGTTGAAGTAGCTGAGGAGATTTTGGAAGCTCATACCAAGGTTGAAGAAAAACAGCAGATATATGTACCTTACAATATCCTTCCTCTTGATCCTGATAGTCAAAATCAGGCGATCATGAGATATCCTGAGATTCATGCTACTGTTTCTGCACTCCGGAACACCAGGGGTCTTCCATGGCCGCAGGGCCACAAGAAGAAAGTAGATGAAGATATTCTAGACTGGCTTCAGGCCATGTTTGGTTTTCAGAAAGATAATGTGGCAAATCAAAGGGAACACTTGATTCTGCTGGTTGCTAATGTCCACATACGGCAATTGCCCAAGCCTGATCAACAGCCCAAGTTGGATGATCGAGCTTTAACGGATGTGATGAAGAAGCTTTTCAAAAATTACAAAAAGTGGTGTAAATACCTGGATCGGAAAAGTAGCCTGTGGTTGCCAACTATTCAGCAGGAAGTACAGCAACGAAAGTTGCTATACATGGGTTTATATCTTCTTATATGGGGAGAAGCTGCAAATTTGAGATTCATGCCAGAATGTCTTTGCTATATTTATCATCATATGGCATTTGAATTGTATGGAATGCTAGCTGGGAGTGTTAGTCCAATGACAGGTGAGCACATAAAGCCAGCCTATGGTGGAGAGGATGAGGCCTTTTTGTCAAAAGTAGTCACTCCAATATATGATACAATAGCAAAGGAGGCTAAAAGGAGCAAAGGAGGAAAATCAAAGCATTCCCAATGGAGAAATTACGACGACTTGAATGAATATTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCAGATGCTGATTTCTTTTGCCCGCCTATCGACGATATTCAAGTTGACAAAGATGAGAGAAAGAAACCGTTCACTGGGGATCGATGGATTGGCAAAGTCAACTTTGTTGAAGTACGCTCATTTTGGCACATTTTCAGAAGTTTTGATAGGATGTGGAGCTTTTATATACTGAGTTTACAGGCAATGATCATTGTTGCTTGGAATGGGTCGGGGGACTTAGGTTCCATGTTTGAGGGTGATGTCTTTAAGAAAGTGCTGAGTATCTTCGTAACTGCTGCCATATTGAAGCTTGCACATGCTGTTCTTGATTTAATTTTGAGCTGGAAGGCTAGGCAAAGCATGTCATTCTTTGTCAAGCTAAGATATGTCTTGAAGGCTGTTTCAGCTGCAGCATGGGTCATTGTTTTACCAGTGACTTACGCCTATAGTTGGAAAGATCCTCCCGGATTTGCACGGACCATCAAAAACTGGTTTGGCAATGGTCCAAGTTCAAATTCCTTGTTCATTTTGGCTGTTGTCATCTACTTATCTCCAAACATGCTCTCTGGATTGTTGTTTATGTTCCCATTCATTCGCCGGTTTCTTGAAAGGTCAAACATAAAGCTTGTGATGCTCATGATGTGGTGGTCTCAGACTCGGCTTTATGTTGGAAGGGGAATGCATGAAAGCTCAGTTTCCCTTTTCAAGTATACCCTTTTTTGGGTTCTCCTGCTTGTGTCAAAATTAGCCTTCAGTTACTATGTAGAGATTAGGCCTCTTGTAAAACCAACTAAAGATATCATGAGGGTTCACATAAGGACCTACCAATGGCATGAGTTCTTCCCTCAAGCTAAGAATAACATTGGTGTGGTGATTGCGCTATGGGCTCCCATTGTTCTTGTATACTTTATGGATACTCAAATTTGGTATGCCATATTTTCAACAATATTCGGGGGTATATATGGTGCATTTCGCCGTCTTGGAGAGATTCGAACACTGGGAATGTTGCGATCTCGATTTGAATCACTGCCAGGTGCTTTTAATAATTTTTTGATTCCAGTGGAAAGGATTGAGCAGACTAAAAAGAAGGGAATACTAAAAGCCACGTTCTCTCGCAAGTTTGATAAGACCCCATCTAGTAAAGAGAAGGAAGCAGCAAAGTTTGCACAGATGTGGAACGAAATAATTAGCAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAGAAGAACTTGTTGCTCGTCCCATATGGGGCTGACCCAGATTTAGCAGACCTCATCCAGTGGCCTCCTTTTCTGCTTGCTAGCAAGATCCCAATAGCACTGGACATGGCAAAAGACAGCAAAGATAAAGACCGTGAGCTGAAAAAGAGAATGAGTACTGACAATTATATGCGCTGTGCTATTCGCGAATGCTATCTCTCTTTTAGAAGTATTATTAACTCTTTGGTCAGTGGAGCTCGTGAAAAGAAGGTTATAAGTGATATCTTCACCATAGTTGACGATCATATTGACAAGGGAAATCTGACAACAGAGTTCAATATGAGTGCTCTTCCAAGTCTCCATGAACAATTTGTTAAGTTAATTGAATATCTGATGGAAAACAAACAAGAGGACAAGGACCAGGTCGTAATTGTCTTGCTTAACATGTTAGAGGTTGTGACAAGAGACATAATGGAGGATGAAATTCCCAACTTGCTAGATTCAAGTCATGGATCATATGGAAAAGATGAGGGAATGACACCCCTTGATCAACGGGATACATATTTCGGTGAACTGAATTTCCCATTCCAAGTGTCCACAAAAACAGCAGATTGGAAAGAAAAGATCAGAAGGCTACATCTATTACTTACAGAGAAAGAGTCTGCTATGGATGTCCCATCCAACTTGGAAGCAAGAAGGCGCATTTCTTTCTTTTCCAACTCGTTGTTTATGGACATGCCTCCCGCACCTAAAGTTCGAAATATGCTTTCGTTCTCGGTTTTGACACCATACCATTCAGAAGAAGTTCTTTTCTCCATAAATCATCTGGAAAGGCAGAATGAAGATGGTGTCTCTATCCTCTTTTACTTGCAGAAGATCTTTCCAGATGAATGGACTAACTTTCTTGAGCGGGTCAAGTGCGAAAATGAGGAAGAACTCAGAGCAAACGATGAATTGGAAGAGAAACTCCGTCTATGGGCATCCTATCGAGGCCAAACATTGACAAAAACTGTACGAGGTATGATGTATTACCGGAAAGCTTTGGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGAGCTAATGGCTGGCTATAAGGCTGCTGAATCAACTATTGAGGAACACTCAAAAGCAGAAGGGTCACTTTTGGCACAATGCCAGGCGGTGGTCGATATGAAATTCTCATATGTTGTGTCATGCCAACAATATGGAATTCACAAGCGATCTGGGGATGCTCGTTCCAAAGACATTTTGAAACTTATGGCAACTTATCCATCTCTTCGAGTAGCTTACATTGATGAGGTAGAGAAAACTAGCGAGGATAAATCCAAAAAGAACGTTTGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACTAAGTCAGTTGATTCTTCTGATCCAGTTCAAAGGTTGGATCAGGATATTTATAGGATAAAGCTTCCTGGACCTGCAATTTTGGGAGAGGGAAAGCCAGAAAATCAAAACCATGCCATCATTTTCACACGTGGGGAAGGCTTGCAAACAATTGATATGAATCAGGATAATTACTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGCAAGAATTTCTTGAGAAGCATGATGGTGTTAGATTCCCGACAATACTTGGACTTAGGGAGCATATTTTCACTGGAAGTGTTTCATCTCTTGCATGGTTTATGTCGAATCAAGAGACTAGTTTTGTAACTATCGGGCAGAGATTGCTGGCTAATCCTCTGAGAGTTAGATTCCACTATGGTCATCCAGATGTATTTGATCGACTGTTTCATCTGACTAGAGGGGGTTTCAATTCTACAGTACGTGAAGGCAGCGTCACTCATCATGAATATATACAAGTAGGTAAAGGACGAGATGTTGGCCTCAACCAAATCTCAATTTTTGAGGCAAAGATAGCTAATGGGAATGGCGAACAGACACTGAGTCGTGACATCTATAGACTTGGGCATCGATTTGATTTCTTCCGAATGCTATCCTGCTACGTCACTACAATTGGTTTCTATTTCAGCACTTTATTGACTGTGCTTACGGTCTATGTTTTTCTCTACGGTCGCCTTTATCTTGTTCTCAGTGGACTTGAAAAAGGATTGAGTACTCAGCGAGGTATTCGAGACAATAAACCTCTTCAAGTAGCTCTTGCTTCTCAGTCTGTTGTGCAAATTGGATTTTTGATGGCCTTGCCTATGGTTATGGAAATTGGTTTGGAAAAGGGGTTCCGTACTGCACTTAGCGATTTTGTATTGATGCAATTACAATTGGCTCCGGTGTTTTTCACATTTTCTCTTGGTACAAAGACCCACTATTATGGAAAGACTTTGCTTCATGGAGGTGCAGAATACAGAGGAACGGGTCGTAGCTTTGTAGTGTTTCATGCCAAGTTTGCTGATAACTACAGGCTCTACTCTAGAAGTCACTTTGTCAAGGGCATTGAGTTATTGATTCTGCTTGTGGTATACCACCTATTTGGCCGTTCATATAAAAGTGGAGTTGTATATATTCTCATTACAATACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGGTTTGAGTGGCAAAAGATTGTTGATGACTGGACAGATTGGAAAAAGTGGATAAACAATCATGGAGGGATTGGTGTATCACCAGACAAGAGCTGGGAATCCTGGTGGGAAAAAGAACATGAGCATCTTCTGTACTCCGGGATACGTGGCATCGTTGCTGAGATATTATTAGCATTGCGCTTTTTCATTTACCAGTATGGGCTAGTCTATCACCTTAACATCACAAACAACAAAAGTTTCCTGGTATATGGTGTTTCGTGGCTCGTGATCATTCTAGTTTTGTGTCTGATGAAGGCTGTGTCTGCTGGAAGGCGACGATTAAGTGCAGACTATCAGCTTTTGTTTCGACTGGTTAAGGGATTTATATTTATTACATTTTTAGCCATCTTCATTACCTTAATAGTGGTCCCTCATATGACACTTCGGGATGTTGTAGTGTGCATTCTTGCCTTCATGCCAACTGGATGGGGATTGCTTTTGATTGCGCAAGCTTGTAAGCCACTAATTAAACGAGCAGGGTTTTGGGAATCAGTTCAAACACTTGCTCGCGGTTATGAAATAATCATTGGATTGCTTCTTTTTACCCCGGTTGCATTCTTGGCTTGGTTTCCGTTTGTTTCCGAATTCCAGACGCGAATGCTCTTCAACCAAGCATTCAGCAGAGGTTTACAGATCTCACGTATTCTTGGAGGACAAAAGAAGGGCTCTCACTCCTCCAACAATAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1936

Amino Acids

223.3

Weight (kDa)

9.08

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 169 8.5e-17 Vta1 like
FKS1_dom1 PF14288 318 - 430 1.9e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 855 - 1043 4.6e-66 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1141 1.3e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1142 - 1742 2.7e-184 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2753
Acc16I TGCGCA 2 cut(s) 53, 5562
Acc36I ACCTGC 1 cut(s) 3946
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 2 cut(s) 1118, 3646
AccBSI CCGCTC 1 cut(s) 3388
AccI GTMKAC 4 cut(s) 1985, 2178, 3831, 4962
AccII CGCG 3 cut(s) 2685, 5625, 5710
AccIII TCCGGA 1 cut(s) 639
AciI CCGC 9 cut(s) 35, 43, 665, 1319, 3222, 3388, 3644, 3854, 5625
AclI AACGTT 1 cut(s) 3819
AcoI YGGCCR 3 cut(s) 28, 662, 4975
AcsI RAATTY 9 cut(s) 1000, 1789, 2196, 2961, 3055, 3659, 3693, 4076, 5699
AcuI CTGAAG 4 cut(s) 257, 695, 2011, 3512
AcvI CACGTG 1 cut(s) 3995
AflIII ACRYGT 2 cut(s) 2922, 3992
AhdI GACNNNNNGTC 1 cut(s) 4431
AhlI ACTAGT 1 cut(s) 4187
AjiI CACGTC 1 cut(s) 242
AjuI GAANNNNNNNTTGG 4 cut(s) 1774, 1806, 5625, 5657
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 6 cut(s) 231, 263, 3042, 3074, 4706, 4738
Alw21I GWGCWC 3 cut(s) 1104, 2737, 2827
Alw26I GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4614
AlwNI CAGNNNCTG 2 cut(s) 1304, 4429
Ama87I CYCGRG 1 cut(s) 330
Aor13HI TCCGGA 1 cut(s) 639
ApoI RAATTY 9 cut(s) 1000, 1789, 2196, 2961, 3055, 3659, 3693, 4076, 5699
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp700I GAANNNNTTC 6 cut(s) 696, 863, 1364, 3282, 4150, 5607
AspLEI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5256, 5563
AspS9I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5482
AsuC2I CCSGG 3 cut(s) 1740, 5215, 5663
AsuHPI GGTGA 7 cut(s) 1109, 1544, 2158, 2758, 3059, 5148, 5279
AsuII TTCGAA 2 cut(s) 2266, 3235
AsuNHI GCTAGC 2 cut(s) 1069, 2579
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5482
BaeGI GKGCMC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 3 cut(s) 2164, 2737, 5783
BauI CACGAG 2 cut(s) 2736, 5339
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 1 cut(s) 647
Bbv12I GWGCWC 3 cut(s) 1104, 2737, 2827
BbvCI CCTCAGC 1 cut(s) 498
BceAI ACGGC 4 cut(s) 43, 809, 2235, 4962
BciVI GTATCC 3 cut(s) 2182, 3028, 5211
BclI TGATCA 4 cut(s) 811, 1482, 3025, 5343
BcnI CCSGG 3 cut(s) 1740, 5215, 5663
BcoDI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4614
BcuI ACTAGT 1 cut(s) 4187
BfmI CTRYAG 5 cut(s) 1683, 1720, 4305, 4444, 4900
BfuAI ACCTGC 1 cut(s) 3946
BfuI GTATCC 3 cut(s) 2182, 3028, 5211
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 2 cut(s) 3354, 5748
BmcAI AGTACT 2 cut(s) 2656, 4603
Bme18I GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5482
BmeRI GACNNNNNGTC 1 cut(s) 4431
BmeT110I CYCGRG 1 cut(s) 330
BmgBI CACGTC 1 cut(s) 242
BmgT120I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5482
BmiI GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4724, 4770, 5484
BmrI ACTGGG 2 cut(s) 1380, 2282
BmsI GCATC 9 cut(s) 316, 1291, 1896, 3467, 3703, 4465, 4743, 5209, 5235
BmtI GCTAGC 2 cut(s) 1073, 2583
BmuI ACTGGG 2 cut(s) 1380, 2282
BpiI GAAGAC 1 cut(s) 647
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 3590, 3622
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 2266, 3235
BpuEI CTTGAG 3 cut(s) 2106, 3404, 4103
BpuMI CCSGG 3 cut(s) 1740, 5215, 5663
Bsa29I ATCGAT 2 cut(s) 1378, 4459
BsaAI YACGTR 4 cut(s) 3995, 4313, 5222, 5756
BsaBI GATNNNNATC 2 cut(s) 2296, 4023
BsaI GGTCTC 2 cut(s) 74, 1929
BsaJI CCNNGG 5 cut(s) 38, 522, 648, 659, 5661
BsaWI WCCGGW 3 cut(s) 639, 3510, 4771
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bse118I RCCGGY 1 cut(s) 1872
Bse3DI GCAATG 3 cut(s) 1485, 1780, 5249
Bse8I GATNNNNATC 2 cut(s) 2296, 4023
BseAI TCCGGA 1 cut(s) 639
BseCI ATCGAT 2 cut(s) 1378, 4459
BseDI CCNNGG 5 cut(s) 38, 522, 648, 659, 5661
BseJI GATNNNNATC 2 cut(s) 2296, 4023
BseMI GCAATG 3 cut(s) 1485, 1780, 5249
BseRI GAGGAG 4 cut(s) 30, 515, 3849, 5779
BseSI GKGCMC 1 cut(s) 415
BseYI CCCAGC 3 cut(s) 65, 1073, 5169
BsgI GTGCAG 6 cut(s) 249, 618, 1317, 4716, 4860, 5421
Bsh1236I CGCG 3 cut(s) 2685, 5625, 5710
Bsh1285I CGRYCG 1 cut(s) 4561
BshNI GGYRCC 1 cut(s) 103
BshVI ATCGAT 2 cut(s) 1378, 4459
BsiEI CGRYCG 1 cut(s) 4561
BsiHKAI GWGCWC 3 cut(s) 1104, 2737, 2827
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 7 cut(s) 640, 1740, 1873, 3511, 4772, 5214, 5663
BslFI GGGAC 4 cut(s) 1523, 2504, 3137, 5468
BsmAI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4614
BsmFI GGGAC 4 cut(s) 1523, 2504, 3137, 5468
Bso31I GGTCTC 2 cut(s) 74, 1929
BsoBI CYCGRG 1 cut(s) 330
Bsp119I TTCGAA 2 cut(s) 2266, 3235
Bsp1286I GDGCHC 6 cut(s) 415, 1104, 2164, 2737, 2827, 5783
Bsp13I TCCGGA 1 cut(s) 639
Bsp19I CCATGG 1 cut(s) 659
Bsp68I TCGCGA 1 cut(s) 2685
BspACI CCGC 9 cut(s) 35, 43, 665, 1319, 3222, 3388, 3644, 3854, 5625
BspDI ATCGAT 2 cut(s) 1378, 4459
BspEI TCCGGA 1 cut(s) 639
BspFNI CGCG 3 cut(s) 2685, 5625, 5710
BspHI TCATGA 4 cut(s) 600, 1911, 2076, 4333
BspLI GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4724, 4770, 5484
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 1 cut(s) 3946
BspOI GCTAGC 2 cut(s) 1073, 2583
BspQI GCTCTTC 3 cut(s) 2832, 3552, 5723
BspT104I TTCGAA 2 cut(s) 2266, 3235
BspT107I GGYRCC 1 cut(s) 103
BspTNI GGTCTC 2 cut(s) 74, 1929
BsrBI CCGCTC 1 cut(s) 3388
BsrDI GCAATG 3 cut(s) 1485, 1780, 5249
BsrFI RCCGGY 1 cut(s) 1872
BssAI RCCGGY 1 cut(s) 1872
BssECI CCNNGG 5 cut(s) 38, 522, 648, 659, 5661
BssNAI GTATAC 3 cut(s) 1986, 2179, 4963
BssSI CACGAG 2 cut(s) 2736, 5339
BssT1I CCWWGG 2 cut(s) 522, 659
Bst1107I GTATAC 3 cut(s) 1986, 2179, 4963
Bst2BI CACGAG 2 cut(s) 2736, 5339
Bst4CI ACNGT 9 cut(s) 628, 1365, 2633, 3490, 4275, 4309, 4531, 4540, 4559
BstAPI GCANNNNNTGC 2 cut(s) 2438, 4213
BstBAI YACGTR 4 cut(s) 3995, 4313, 5222, 5756
BstBI TTCGAA 2 cut(s) 2266, 3235
BstDSI CCRYGG 1 cut(s) 659
BstFNI CGCG 3 cut(s) 2685, 5625, 5710
BstHHI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5256, 5563
BstMAI GTCTC 7 cut(s) 74, 1929, 2840, 2937, 3334, 4178, 4614
BstMCI CGRYCG 1 cut(s) 4561
BstNSI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
BstSFI CTRYAG 5 cut(s) 1683, 1720, 4305, 4444, 4900
BstSLI GKGCMC 1 cut(s) 415
BstUI CGCG 3 cut(s) 2685, 5625, 5710
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5748
BstXI CCANNNNNNTGG 6 cut(s) 65, 3369, 3689, 4814, 4973, 5541
BstYI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5748
BstZ17I GTATAC 3 cut(s) 1986, 2179, 4963
Bsu15I ATCGAT 2 cut(s) 1378, 4459
BsuI GTATCC 3 cut(s) 2182, 3028, 5211
BsuTUI ATCGAT 2 cut(s) 1378, 4459
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 242
BtsI GCAGTG 1 cut(s) 2300
BtuMI TCGCGA 1 cut(s) 2685
BveI ACCTGC 1 cut(s) 3946
CaiI CAGNNNCTG 2 cut(s) 1304, 4429
CciI TCATGA 4 cut(s) 600, 1911, 2076, 4333
CfoI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5256, 5563
Cfr10I RCCGGY 1 cut(s) 1872
Cfr13I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5482
ClaI ATCGAT 2 cut(s) 1378, 4459
CseI GACGC 3 cut(s) 266, 4312, 5716
CsiI ACCWGGT 1 cut(s) 2898
DraI TTTAAA 1 cut(s) 259
DriI GACNNNNNGTC 1 cut(s) 4431
EaeI YGGCCR 3 cut(s) 28, 662, 4975
Eam1105I GACNNNNNGTC 1 cut(s) 4431
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 2735
Eco130I CCWWGG 2 cut(s) 522, 659
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3533
Eco24I GRGCYC 3 cut(s) 2164, 2737, 5783
Eco31I GGTCTC 2 cut(s) 74, 1929
Eco32I GATATC 3 cut(s) 608, 2074, 2761
Eco47I GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5482
Eco53kI GAGCTC 1 cut(s) 2735
Eco57I CTGAAG 4 cut(s) 257, 695, 2011, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 2735
EcoO109I RGGNCCY 1 cut(s) 2094
EcoRI GAATTC 2 cut(s) 3693, 5699
EcoRV GATATC 3 cut(s) 608, 2074, 2761
EcoT14I CCWWGG 2 cut(s) 522, 659
EcoT22I ATGCAT 1 cut(s) 1959
EcoT38I GRGCYC 3 cut(s) 2164, 2737, 5783
ErhI CCWWGG 2 cut(s) 522, 659
FalI AAGNNNNNCTT 8 cut(s) 3270, 3302, 3609, 3641, 4065, 4097, 4629, 4661
FaqI GGGAC 4 cut(s) 1523, 2504, 3137, 5468
FauI CCCGC 3 cut(s) 1326, 3229, 3381
FauNDI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5490
FbaI TGATCA 4 cut(s) 811, 1482, 3025, 5343
FblI GTMKAC 4 cut(s) 1985, 2178, 3831, 4962
FriOI GRGCYC 3 cut(s) 2164, 2737, 5783
FspI TGCGCA 2 cut(s) 53, 5562
GlaI GCGC 6 cut(s) 53, 2154, 2672, 3179, 5255, 5562
GsaI CCCAGC 3 cut(s) 69, 1077, 5173
HapII CCGG 7 cut(s) 640, 1740, 1873, 3511, 4772, 5214, 5663
HgaI GACGC 3 cut(s) 266, 4312, 5716
HhaI GCGC 6 cut(s) 54, 2155, 2673, 3180, 5256, 5563
Hin6I GCGC 6 cut(s) 52, 2153, 2671, 3178, 5254, 5561
HinP1I GCGC 6 cut(s) 52, 2153, 2671, 3178, 5254, 5561
HincII GTYRAC 3 cut(s) 1342, 1396, 2776
HindII GTYRAC 3 cut(s) 1342, 1396, 2776
HindIII AAGCTT 7 cut(s) 378, 860, 1583, 1898, 3516, 3926, 5565
HpaII CCGG 7 cut(s) 640, 1740, 1873, 3511, 4772, 5214, 5663
HphI GGTGA 7 cut(s) 1109, 1544, 2158, 2758, 3059, 5148, 5279
Hpy99I CGWCG 3 cut(s) 1241, 1331, 5394
HpyCH4III ACNGT 9 cut(s) 628, 1365, 2633, 3490, 4275, 4309, 4531, 4540, 4559
HpyCH4IV ACGT 8 cut(s) 241, 2381, 3819, 3994, 4312, 4491, 5221, 5755
HpySE526I ACGT 8 cut(s) 241, 2381, 3819, 3994, 4312, 4491, 5221, 5755
HspAI GCGC 6 cut(s) 52, 2153, 2671, 3178, 5254, 5561
Kpn2I TCCGGA 1 cut(s) 639
Ksp22I TGATCA 4 cut(s) 811, 1482, 3025, 5343
LguI GCTCTTC 3 cut(s) 2832, 3552, 5723
LweI GCATC 9 cut(s) 316, 1291, 1896, 3467, 3703, 4465, 4743, 5209, 5235
MabI ACCWGGT 1 cut(s) 2898
MaeII ACGT 8 cut(s) 241, 2381, 3819, 3994, 4312, 4491, 5221, 5755
MbiI CCGCTC 1 cut(s) 3388
MfeI CAATTG 4 cut(s) 797, 4014, 4500, 4763
MflI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5748
MhlI GDGCHC 6 cut(s) 415, 1104, 2164, 2737, 2827, 5783
MlyI GAGTC 4 cut(s) 167, 1924, 3146, 4441
MmeI TCCRAC 8 cut(s) 807, 1706, 1924, 3183, 3219, 3882, 3887, 5017
Mph1103I ATGCAT 1 cut(s) 1959
MroI TCCGGA 1 cut(s) 639
MroXI GAANNNNTTC 6 cut(s) 696, 863, 1364, 3282, 4150, 5607
MslI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4812, 4836
MspA1I CMGCKG 1 cut(s) 1682
MspI CCGG 7 cut(s) 640, 1740, 1873, 3511, 4772, 5214, 5663
MunI CAATTG 4 cut(s) 797, 4014, 4500, 4763
MvnI CGCG 3 cut(s) 2685, 5625, 5710
NciI CCSGG 3 cut(s) 1740, 5215, 5663
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5490
NheI GCTAGC 2 cut(s) 1069, 2579
NlaIV GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4724, 4770, 5484
NmeAIII GCCGAG 2 cut(s) 63, 1912
NmuCI GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4324, 4436, 4492, 4919
NruI TCGCGA 1 cut(s) 2685
NsbI TGCGCA 2 cut(s) 53, 5562
NsiI ATGCAT 1 cut(s) 1959
NspI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
NspV TTCGAA 2 cut(s) 2266, 3235
OliI CACNNNNGTG 1 cut(s) 2446
PaeI GCATGC 1 cut(s) 392
PagI TCATGA 4 cut(s) 600, 1911, 2076, 4333
PceI AGGCCT 3 cut(s) 1136, 2050, 3533
PciI ACATGT 1 cut(s) 2922
PciSI GCTCTTC 3 cut(s) 2832, 3552, 5723
PdmI GAANNNNTTC 6 cut(s) 696, 863, 1364, 3282, 4150, 5607
PflFI GACNNNGTC 1 cut(s) 2900
PflMI CCANNNNNTGG 2 cut(s) 1118, 3646
PfoI TCCNGGA 5 cut(s) 161, 1738, 3537, 3931, 5213
PleI GAGTC 4 cut(s) 166, 1924, 3145, 4440
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 4 cut(s) 166, 1924, 3145, 4440
Ppu21I YACGTR 4 cut(s) 3995, 4313, 5222, 5756
PpuMI RGGWCCY 1 cut(s) 2094
PscI ACATGT 1 cut(s) 2922
PsiI TTATAA 1 cut(s) 2753
Psp124BI GAGCTC 1 cut(s) 2737
Psp1406I AACGTT 1 cut(s) 3819
Psp5II RGGWCCY 1 cut(s) 2094
PspCI CACGTG 1 cut(s) 3995
PspFI CCCAGC 3 cut(s) 65, 1073, 5169
PspN4I GGNNCC 7 cut(s) 105, 1519, 2163, 3858, 4724, 4770, 5484
PspPI GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5482
PspPPI RGGWCCY 1 cut(s) 2094
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 2 cut(s) 1304, 4429
PsuI RGATCY 6 cut(s) 223, 1732, 2482, 2586, 3354, 5748
PsyI GACNNNGTC 1 cut(s) 2900
PvuII CAGCTG 1 cut(s) 1682
RruI TCGCGA 1 cut(s) 2685
RseI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4812, 4836
SacI GAGCTC 1 cut(s) 2737
SapI GCTCTTC 3 cut(s) 2832, 3552, 5723
Sau96I GGNCC 7 cut(s) 670, 1752, 1778, 2094, 2896, 3935, 5482
ScaI AGTACT 2 cut(s) 2656, 4603
SchI GAGTC 4 cut(s) 167, 1924, 3146, 4441
SduI GDGCHC 6 cut(s) 415, 1104, 2164, 2737, 2827, 5783
SexAI ACCWGGT 1 cut(s) 2898
SfaNI GCATC 9 cut(s) 316, 1291, 1896, 3467, 3703, 4465, 4743, 5209, 5235
SfcI CTRYAG 5 cut(s) 1683, 1720, 4305, 4444, 4900
SfuI TTCGAA 2 cut(s) 2266, 3235
SinI GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5482
SmiMI CAYNNNNRTG 6 cut(s) 2141, 2446, 4095, 4257, 4812, 4836
SmlI CTYRAG 3 cut(s) 2121, 3383, 4082
SmoI CTYRAG 3 cut(s) 2121, 3383, 4082
SpeI ACTAGT 1 cut(s) 4187
SphI GCATGC 1 cut(s) 392
SseBI AGGCCT 3 cut(s) 1136, 2050, 3533
SsiI CCGC 9 cut(s) 35, 43, 665, 1319, 3222, 3388, 3644, 3854, 5625
SspI AATATT 2 cut(s) 1253, 2223
SstI GAGCTC 1 cut(s) 2737
StuI AGGCCT 3 cut(s) 1136, 2050, 3533
StyI CCWWGG 2 cut(s) 522, 659
TaaI ACNGT 9 cut(s) 628, 1365, 2633, 3490, 4275, 4309, 4531, 4540, 4559
TaiI ACGT 8 cut(s) 244, 2384, 3822, 3997, 4315, 4494, 5224, 5758
TatI WGTACW 4 cut(s) 940, 2654, 4601, 5208
TauI GCSGC 3 cut(s) 37, 667, 3857
TseFI GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4324, 4436, 4492, 4919
Tsp45I GTSAC 8 cut(s) 1153, 1708, 2935, 3621, 4324, 4436, 4492, 4919
TspGWI ACGGA 7 cut(s) 128, 494, 860, 1765, 3437, 4715, 5676
Tth111I GACNNNGTC 1 cut(s) 2900
Van91I CCANNNNNTGG 2 cut(s) 1118, 3646
VpaK11BI GGWCC 6 cut(s) 1752, 1778, 2094, 2896, 3935, 5482
XapI RAATTY 9 cut(s) 1000, 1789, 2196, 2961, 3055, 3659, 3693, 4076, 5699
XbaI TCTAGA 3 cut(s) 280, 700, 4913
XceI RCATGY 6 cut(s) 392, 1595, 1639, 1837, 2926, 3217
XcmI CCANNNNNNNNNTGG 2 cut(s) 3301, 5166
XmiI GTMKAC 4 cut(s) 1985, 2178, 3831, 4962
XmnI GAANNNNTTC 6 cut(s) 696, 863, 1364, 3282, 4150, 5607
ZrmI AGTACT 2 cut(s) 2656, 4603
Zsp2I ATGCAT 1 cut(s) 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.