Rw1G000180

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002059
Physical Location & Seq
Forward (+)
240748 .. 260111
19364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000180.1

Sequence Viewer

Length: 5847 bp
ATGGCATATAGAAGGGGCTTTGACCAGCAGCATCCGCAGAGGCCGATTCTGCGGACTCAGACTGCCGGCCCCGGCAGTTTTGGAGAGCCGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCCCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCCAGTGGCCGTGGTGTTCGCCAGTTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCAAGAGTACTACAGAAAATACATTCAAGCTTTGCACACTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTTCTATTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCTCAGGCTAAGGTTGAAGAAAAACAACAAATATATCTACCTTACAATATTCTTCCTCTTGATCGTGATAGTCGAAATCAGGCAATCATGAGATATCCCGAGATTCTTGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGCCAAAGGACTACAAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAGGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTATTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCCAAGTTGGATGATCGTGCTTTAACAGTAGTGATGAAGAAACTTTTCAAAAATTACAAAAGATGGTGCAAGTACTTGGGTCGCAAAAGCAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTAGAGCAGCGGAAATTACTATACATGGGTTTATATCTTCTTATCTGGGGAGAGGCTGCCAACTTGAGATTCATGCCAGAATGCCTTTGCTACATTTATCATCATATGGCATTTGAATTATATGGAATGCTAGCCGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCGAAGCATTCTCAATGGAGAAATTATGATGACCTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGATGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACTATACACCGGGGATCGATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATACTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTACAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCGCAAGCTGTTCTCGATCTAATTCTAAGCTGGAAAGCTAGGCGAAGCATGCCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCCGTGACTTATGCATTTAGCTGGAAAAATCCCTCTGGGTTTGCACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTGTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGTTTCTTGAAAGATCAAACTATAGGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTGGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGACAAGATCCCATCTAATATAGAGAATGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCACTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGAACCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAATGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCTGTTAGAGAGTGCTATCTTTCGTTTAGAAGTATTATCAATGTTTTAGTCCAAGGAGAACGCGAAAAGATGGTTATAAATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACACTGACGAAGGAAGTTAAATTGAGTGCTCTCCCAAGCCTCCACGAACAATTTATTAAGCTAATTGATTATCTGTTGACAAACAAAAGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGATGATGACATTACCACCTTGGTAGATTCAAACCATGGTGGATCATATGGAAATGATGGGGGAATGAGACCTCTTAATCAACAAGATACATACTTCAGTGAACTGAATTTCCCAGTGACGCAACAATCAGAGGCTTGGAAAGAAAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGAAATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCGGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAACGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACTAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAACTCAGAGCAAATGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTATAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGAAAGGCCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGAAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACAAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGTCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCCCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTCATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCACTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTCGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAACGGTGAGCAGACACTGAGTCGTGACATCTATAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTAATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTTCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTCGGTACAAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACTGTACAGATATGGTTCATGGTTGGAACATGGCTTTTCGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATTGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTACTCTGGGATGCGCGGTATCATTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTCGTATATCACCTCAACATTACAGAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATTGTTGTTTTGTTGCTGATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTTTTGTTTCGACTTGTTAAGGGATGTATATTTATTACACTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATCGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTTTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCAGTCGGTTCAAACACTTGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCTTGGTTCCCTTTTGTTTCTGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGTCCCCGCGCTAGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1948

Amino Acids

225.11

Weight (kDa)

9.16

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 171 3e-15 Vta1 like
FKS1_dom1 PF14288 317 - 429 8.8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 852 - 1041 2.6e-62 Callose synthase 1 domain
Glucan_synthase PF02364 1049 - 1139 4e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1143 - 1842 4.1e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2750
AasI GACNNNNNNGTC 1 cut(s) 1532
Acc16I TGCGCA 1 cut(s) 1587
Acc36I ACCTGC 1 cut(s) 4325
AccB1I GGYRCC 1 cut(s) 1419
AccB7I CCANNNNNTGG 1 cut(s) 1115
AccBSI CCGCTC 1 cut(s) 4623
AccI GTMKAC 3 cut(s) 1982, 3825, 5307
AccII CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
AclWI GGATC 8 cut(s) 221, 1380, 2392, 2484, 2572, 2998, 3091, 3875
AcoI YGGCCR 3 cut(s) 241, 659, 4240
AcsI RAATTY 9 cut(s) 2193, 2387, 2429, 2757, 3055, 3653, 4070, 4928, 5741
AcuI CTGAAG 4 cut(s) 692, 3028, 3420, 3506
AcvI CACGTG 1 cut(s) 3989
AdeI CACNNNGTG 1 cut(s) 4781
AfeI AGCGCT 1 cut(s) 3513
AflII CTTAAG 1 cut(s) 4315
AflIII ACRYGT 2 cut(s) 2919, 3986
AhdI GACNNNNNGTC 2 cut(s) 4406, 4473
AhlI ACTAGT 2 cut(s) 2459, 4178
AjuI GAANNNNNNNTTGG 4 cut(s) 989, 1021, 1771, 1803
AleI CACNNNNGTG 1 cut(s) 2440
AloI GAACNNNNNNTCC 2 cut(s) 3042, 3074
Alw21I GWGCWC 6 cut(s) 412, 759, 1101, 2085, 2824, 5526
Alw26I GTCTC 5 cut(s) 1926, 3010, 3328, 3840, 4169
Alw44I GTGCAC 2 cut(s) 408, 2081
AlwI GGATC 8 cut(s) 221, 1380, 2392, 2484, 2572, 2998, 3091, 3875
AlwNI CAGNNNCTG 2 cut(s) 482, 4471
Ama87I CYCGRG 2 cut(s) 608, 2118
Aor51HI AGCGCT 1 cut(s) 3513
ApaLI GTGCAC 2 cut(s) 408, 2081
ApoI RAATTY 9 cut(s) 2193, 2387, 2429, 2757, 3055, 3653, 4070, 4928, 5741
AseI ATTAAT 1 cut(s) 4793
Asp700I GAANNNNTTC 4 cut(s) 693, 860, 3276, 4071
AspA2I CCTAGG 1 cut(s) 5813
AspLEI GCGC 7 cut(s) 1588, 1964, 2670, 3514, 5265, 5298, 5831
AsuC2I CCSGG 4 cut(s) 72, 1072, 1369, 5705
AsuHPI GGTGA 9 cut(s) 1106, 1133, 1541, 3287, 3716, 4472, 4528, 5321, 5395
AsuII TTCGAA 2 cut(s) 2012, 4660
AsuNHI GCTAGC 4 cut(s) 1066, 2570, 4211, 5831
AvaI CYCGRG 2 cut(s) 608, 2118
AvaII GGWCC 6 cut(s) 1775, 2893, 2941, 3898, 3929, 5822
AvrII CCTAGG 1 cut(s) 5813
AxyI CCTNAGG 1 cut(s) 2331
BaeGI GKGCMC 2 cut(s) 412, 2085
BaeI ACNNNNGTAYC 2 cut(s) 5338, 5371
BalI TGGCCA 2 cut(s) 661, 4242
BanI GGYRCC 1 cut(s) 1419
BanII GRGCYC 2 cut(s) 2161, 5322
BarI GAAGNNNNNNTAC 4 cut(s) 2192, 2224, 3160, 3192
BauI CACGAG 4 cut(s) 333, 3711, 5237, 5664
BbrPI CACGTG 1 cut(s) 3989
BbsI GAAGAC 4 cut(s) 696, 3279, 3429, 3801
Bbv12I GWGCWC 6 cut(s) 412, 759, 1101, 2085, 2824, 5526
BbvCI CCTCAGC 1 cut(s) 495
BceAI ACGGC 3 cut(s) 73, 228, 806
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciVI GTATCC 2 cut(s) 2179, 5366
BclI TGATCA 5 cut(s) 808, 1479, 2776, 4308, 5385
BcnI CCSGG 4 cut(s) 72, 1072, 1369, 5705
BcoDI GTCTC 5 cut(s) 1926, 3010, 3328, 3840, 4169
BcuI ACTAGT 2 cut(s) 2459, 4178
BfmI CTRYAG 8 cut(s) 364, 1170, 1680, 1891, 2583, 4486, 4701, 4942
BfoI RGCGCY 1 cut(s) 3515
BfrI CTTAAG 1 cut(s) 4315
BfuAI ACCTGC 1 cut(s) 4325
BfuI GTATCC 2 cut(s) 2179, 5366
BglII AGATCT 2 cut(s) 3553, 4422
BlnI CCTAGG 1 cut(s) 5813
BmcAI AGTACT 3 cut(s) 362, 890, 4645
Bme18I GGWCC 6 cut(s) 1775, 2893, 2941, 3898, 3929, 5822
BmeRI GACNNNNNGTC 2 cut(s) 4406, 4473
BmeT110I CYCGRG 2 cut(s) 608, 2118
BmrI ACTGGG 1 cut(s) 3056
BmsI GCATC 8 cut(s) 40, 319, 1288, 1893, 3461, 3697, 5250, 5251
BmtI GCTAGC 4 cut(s) 1070, 2574, 4215, 5835
BmuI ACTGGG 1 cut(s) 3056
BpiI GAAGAC 4 cut(s) 696, 3279, 3429, 3801
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 1394, 1426
BpmI CTGGAG 1 cut(s) 2291
Bpu10I CCTNAGC 4 cut(s) 495, 513, 519, 4115
Bpu14I TTCGAA 2 cut(s) 2012, 4660
BpuEI CTTGAG 1 cut(s) 1021
BpuMI CCSGG 4 cut(s) 72, 1072, 1369, 5705
Bsa29I ATCGAT 1 cut(s) 1375
BsaAI YACGTR 3 cut(s) 3989, 4355, 5798
BsaBI GATNNNNATC 3 cut(s) 2293, 3552, 3909
BsaI GGTCTC 2 cut(s) 1926, 3010
BsaWI WCCGGW 1 cut(s) 3504
BsaXI ACNNNNNCTCC 6 cut(s) 1066, 1096, 3116, 3146, 4409, 4439
Bse118I RCCGGY 3 cut(s) 65, 1869, 2247
Bse21I CCTNAGG 1 cut(s) 2331
Bse3DI GCAATG 4 cut(s) 1482, 1578, 4727, 5291
Bse8I GATNNNNATC 3 cut(s) 2293, 3552, 3909
BseCI ATCGAT 1 cut(s) 1375
BseJI GATNNNNATC 3 cut(s) 2293, 3552, 3909
BseMI GCAATG 4 cut(s) 1482, 1578, 4727, 5291
BseRI GAGGAG 4 cut(s) 512, 1142, 2348, 3843
BseSI GKGCMC 2 cut(s) 412, 2085
BsgI GTGCAG 5 cut(s) 615, 3201, 4758, 4902, 5463
Bsh1236I CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
Bsh1285I CGRYCG 2 cut(s) 3591, 4603
BshNI GGYRCC 1 cut(s) 1419
BshVI ATCGAT 1 cut(s) 1375
BsiEI CGRYCG 2 cut(s) 3591, 4603
BsiHKAI GWGCWC 6 cut(s) 412, 759, 1101, 2085, 2824, 5526
BsiHKCI CYCGRG 2 cut(s) 608, 2118
BsiSI CCGG 8 cut(s) 66, 72, 1071, 1368, 1870, 2248, 3505, 5705
BslFI GGGAC 4 cut(s) 2376, 2498, 2954, 5808
BsmAI GTCTC 5 cut(s) 1926, 3010, 3328, 3840, 4169
BsmBI CGTCTC 1 cut(s) 3840
BsmFI GGGAC 4 cut(s) 2376, 2498, 2954, 5808
Bso31I GGTCTC 2 cut(s) 1926, 3010
BsoBI CYCGRG 2 cut(s) 608, 2118
Bsp119I TTCGAA 2 cut(s) 2012, 4660
Bsp1286I GDGCHC 8 cut(s) 412, 759, 1101, 2085, 2161, 2824, 5322, 5526
Bsp1407I TGTACA 1 cut(s) 5059
Bsp19I CCATGG 1 cut(s) 2983
BspDI ATCGAT 1 cut(s) 1375
BspFNI CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
BspHI TCATGA 3 cut(s) 597, 2073, 4375
BspMAI CTGCAG 1 cut(s) 1684
BspMI ACCTGC 1 cut(s) 4325
BspOI GCTAGC 4 cut(s) 1070, 2574, 4215, 5835
BspPI GGATC 8 cut(s) 221, 1380, 2392, 2484, 2572, 2998, 3091, 3875
BspT104I TTCGAA 2 cut(s) 2012, 4660
BspT107I GGYRCC 1 cut(s) 1419
BspTI CTTAAG 1 cut(s) 4315
BspTNI GGTCTC 2 cut(s) 1926, 3010
BsrBI CCGCTC 1 cut(s) 4623
BsrDI GCAATG 4 cut(s) 1482, 1578, 4727, 5291
BsrFI RCCGGY 3 cut(s) 65, 1869, 2247
BsrGI TGTACA 1 cut(s) 5059
BssAI RCCGGY 3 cut(s) 65, 1869, 2247
BssNAI GTATAC 1 cut(s) 1983
BssSI CACGAG 4 cut(s) 333, 3711, 5237, 5664
BssT1I CCWWGG 4 cut(s) 2725, 2967, 2983, 5813
Bst1107I GTATAC 1 cut(s) 1983
Bst2BI CACGAG 4 cut(s) 333, 3711, 5237, 5664
Bst6I CTCTTC 7 cut(s) 1119, 1939, 2120, 2466, 3426, 3759, 4680
BstAFI CTTAAG 1 cut(s) 4315
BstAPI GCANNNNNTGC 2 cut(s) 179, 2432
BstAUI TGTACA 1 cut(s) 5059
BstBAI YACGTR 3 cut(s) 3989, 4355, 5798
BstBI TTCGAA 2 cut(s) 2012, 4660
BstDSI CCRYGG 2 cut(s) 244, 2983
BstENI CCTNNNNNAGG 1 cut(s) 1645
BstFNI CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
BstH2I RGCGCY 1 cut(s) 3515
BstHHI GCGC 7 cut(s) 1588, 1964, 2670, 3514, 5265, 5298, 5831
BstMAI GTCTC 5 cut(s) 1926, 3010, 3328, 3840, 4169
BstMCI CGRYCG 2 cut(s) 3591, 4603
BstNSI RCATGY 3 cut(s) 1636, 1834, 2923
BstSFI CTRYAG 8 cut(s) 364, 1170, 1680, 1891, 2583, 4486, 4701, 4942
BstSLI GKGCMC 2 cut(s) 412, 2085
BstUI CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
BstV2I GAAGAC 4 cut(s) 696, 3279, 3429, 3801
BstX2I RGATCY 7 cut(s) 226, 2397, 2476, 2577, 3096, 3553, 4422
BstXI CCANNNNNNTGG 5 cut(s) 3363, 3683, 3811, 5015, 5583
BstYI RGATCY 7 cut(s) 226, 2397, 2476, 2577, 3096, 3553, 4422
BstZ17I GTATAC 1 cut(s) 1983
Bsu15I ATCGAT 1 cut(s) 1375
Bsu36I CCTNAGG 1 cut(s) 2331
BsuI GTATCC 2 cut(s) 2179, 5366
BsuTUI ATCGAT 1 cut(s) 1375
BtgI CCRYGG 2 cut(s) 244, 2983
BtsI GCAGTG 2 cut(s) 2297, 4144
BveI ACCTGC 1 cut(s) 4325
CaiI CAGNNNCTG 2 cut(s) 482, 4471
CciI TCATGA 3 cut(s) 597, 2073, 4375
CfoI GCGC 7 cut(s) 1588, 1964, 2670, 3514, 5265, 5298, 5831
Cfr10I RCCGGY 3 cut(s) 65, 1869, 2247
ClaI ATCGAT 1 cut(s) 1375
CseI GACGC 1 cut(s) 3076
CsiI ACCWGGT 1 cut(s) 2895
DraI TTTAAA 2 cut(s) 262, 1540
DraIII CACNNNGTG 1 cut(s) 4781
DrdI GACNNNNNNGTC 1 cut(s) 1532
DriI GACNNNNNGTC 2 cut(s) 4406, 4473
DseDI GACNNNNNNGTC 1 cut(s) 1532
EaeI YGGCCR 3 cut(s) 241, 659, 4240
Eam1104I CTCTTC 7 cut(s) 1119, 1939, 2120, 2466, 3426, 3759, 4680
Eam1105I GACNNNNNGTC 2 cut(s) 4406, 4473
EarI CTCTTC 7 cut(s) 1119, 1939, 2120, 2466, 3426, 3759, 4680
EciI GGCGGA 1 cut(s) 3431
Eco130I CCWWGG 4 cut(s) 2725, 2967, 2983, 5813
Eco147I AGGCCT 3 cut(s) 1133, 2047, 3527
Eco24I GRGCYC 2 cut(s) 2161, 5322
Eco31I GGTCTC 2 cut(s) 1926, 3010
Eco32I GATATC 2 cut(s) 605, 2071
Eco47I GGWCC 6 cut(s) 1775, 2893, 2941, 3898, 3929, 5822
Eco47III AGCGCT 1 cut(s) 3513
Eco57I CTGAAG 4 cut(s) 692, 3028, 3420, 3506
Eco72I CACGTG 1 cut(s) 3989
Eco81I CCTNAGG 1 cut(s) 2331
Eco88I CYCGRG 2 cut(s) 608, 2118
EcoNI CCTNNNNNAGG 1 cut(s) 1645
EcoO109I RGGNCCY 3 cut(s) 2941, 5810, 5822
EcoRI GAATTC 1 cut(s) 5741
EcoRV GATATC 2 cut(s) 605, 2071
EcoT14I CCWWGG 4 cut(s) 2725, 2967, 2983, 5813
EcoT22I ATGCAT 3 cut(s) 205, 1717, 1956
EcoT38I GRGCYC 2 cut(s) 2161, 5322
ErhI CCWWGG 4 cut(s) 2725, 2967, 2983, 5813
Esp3I CGTCTC 1 cut(s) 3840
FaqI GGGAC 4 cut(s) 2376, 2498, 2954, 5808
FauI CCCGC 2 cut(s) 3940, 5834
FauNDI CATATG 4 cut(s) 1041, 2995, 3661, 5532
FbaI TGATCA 5 cut(s) 808, 1479, 2776, 4308, 5385
FblI GTMKAC 3 cut(s) 1982, 3825, 5307
FriOI GRGCYC 2 cut(s) 2161, 5322
FspI TGCGCA 1 cut(s) 1587
GlaI GCGC 7 cut(s) 1587, 1963, 2669, 3513, 5264, 5297, 5830
GsuI CTGGAG 1 cut(s) 2291
HaeII RGCGCY 1 cut(s) 3515
HapII CCGG 8 cut(s) 66, 72, 1071, 1368, 1870, 2248, 3505, 5705
HgaI GACGC 1 cut(s) 3076
HhaI GCGC 7 cut(s) 1588, 1964, 2670, 3514, 5265, 5298, 5831
Hin6I GCGC 7 cut(s) 1586, 1962, 2668, 3512, 5263, 5296, 5829
HinP1I GCGC 7 cut(s) 1586, 1962, 2668, 3512, 5263, 5296, 5829
HincII GTYRAC 4 cut(s) 1339, 2871, 3646, 4569
HindII GTYRAC 4 cut(s) 1339, 2871, 3646, 4569
HindIII AAGCTT 5 cut(s) 381, 1580, 3920, 4296, 5607
HpaI GTTAAC 1 cut(s) 4569
HpaII CCGG 8 cut(s) 66, 72, 1071, 1368, 1870, 2248, 3505, 5705
HphI GGTGA 9 cut(s) 1106, 1133, 1541, 3287, 3716, 4472, 4528, 5321, 5395
Hpy99I CGWCG 1 cut(s) 5436
HpyCH4IV ACGT 5 cut(s) 280, 3276, 3988, 4354, 5797
HpySE526I ACGT 5 cut(s) 280, 3276, 3988, 4354, 5797
HspAI GCGC 7 cut(s) 1586, 1962, 2668, 3512, 5263, 5296, 5829
KflI GGGWCCC 1 cut(s) 5822
KroI GCCGGC 2 cut(s) 65, 2247
KroNI GCCGGC 2 cut(s) 67, 2249
Ksp22I TGATCA 5 cut(s) 808, 1479, 2776, 4308, 5385
KspAI GTTAAC 1 cut(s) 4569
LmnI GCTCC 7 cut(s) 754, 1191, 1449, 2164, 3856, 4120, 5101
LweI GCATC 8 cut(s) 40, 319, 1288, 1893, 3461, 3697, 5250, 5251
MabI ACCWGGT 1 cut(s) 2895
MaeII ACGT 5 cut(s) 280, 3276, 3988, 4354, 5797
MaeIII GTNAC 9 cut(s) 1150, 1705, 2027, 2129, 2932, 3064, 4186, 4366, 4478
MbiI CCGCTC 1 cut(s) 4623
MfeI CAATTG 3 cut(s) 2163, 3867, 4805
MflI RGATCY 7 cut(s) 226, 2397, 2476, 2577, 3096, 3553, 4422
MhlI GDGCHC 8 cut(s) 412, 759, 1101, 2085, 2161, 2824, 5322, 5526
MlsI TGGCCA 2 cut(s) 661, 4242
MluNI TGGCCA 2 cut(s) 661, 4242
MlyI GAGTC 5 cut(s) 49, 2260, 3140, 4483, 4840
MmeI TCCRAC 6 cut(s) 72, 804, 1921, 3213, 3881, 5059
Mox20I TGGCCA 2 cut(s) 661, 4242
Mph1103I ATGCAT 3 cut(s) 205, 1717, 1956
MroNI GCCGGC 2 cut(s) 65, 2247
MroXI GAANNNNTTC 4 cut(s) 693, 860, 3276, 4071
MscI TGGCCA 2 cut(s) 661, 4242
MslI CAYNNNNRTG 5 cut(s) 2078, 2440, 4248, 4878, 5531
Msp20I TGGCCA 2 cut(s) 661, 4242
MspA1I CMGCKG 3 cut(s) 946, 1679, 5423
MspCI CTTAAG 1 cut(s) 4315
MspI CCGG 8 cut(s) 66, 72, 1071, 1368, 1870, 2248, 3505, 5705
MunI CAATTG 3 cut(s) 2163, 3867, 4805
MvnI CGCG 5 cut(s) 1964, 2736, 5265, 5752, 5829
NaeI GCCGGC 2 cut(s) 67, 2249
NciI CCSGG 4 cut(s) 72, 1072, 1369, 5705
NcoI CCATGG 1 cut(s) 2983
NdeI CATATG 4 cut(s) 1041, 2995, 3661, 5532
NgoMIV GCCGGC 2 cut(s) 65, 2247
NheI GCTAGC 4 cut(s) 1066, 2570, 4211, 5831
NmeAIII GCCGAG 1 cut(s) 1909
NmuCI GTSAC 5 cut(s) 1705, 2932, 3064, 4366, 4478
NsbI TGCGCA 1 cut(s) 1587
NsiI ATGCAT 3 cut(s) 205, 1717, 1956
NspI RCATGY 3 cut(s) 1636, 1834, 2923
NspV TTCGAA 2 cut(s) 2012, 4660
OliI CACNNNNGTG 1 cut(s) 2440
PaeI GCATGC 1 cut(s) 1636
PaeR7I CTCGAG 1 cut(s) 2118
PagI TCATGA 3 cut(s) 597, 2073, 4375
PceI AGGCCT 3 cut(s) 1133, 2047, 3527
PciI ACATGT 1 cut(s) 2919
PcsI WCGNNNNNNNCGW 2 cut(s) 580, 4657
PdiI GCCGGC 2 cut(s) 67, 2249
PdmI GAANNNNTTC 4 cut(s) 693, 860, 3276, 4071
PflMI CCANNNNNTGG 1 cut(s) 1115
PfoI TCCNGGA 2 cut(s) 3531, 3925
Ple19I CGATCG 1 cut(s) 3591
PleI GAGTC 5 cut(s) 49, 2260, 3139, 4482, 4840
PmaCI CACGTG 1 cut(s) 3989
PmlI CACGTG 1 cut(s) 3989
PpsI GAGTC 5 cut(s) 49, 2260, 3139, 4482, 4840
Ppu21I YACGTR 3 cut(s) 3989, 4355, 5798
PpuMI RGGWCCY 2 cut(s) 2941, 5822
PscI ACATGT 1 cut(s) 2919
PshBI ATTAAT 1 cut(s) 4793
PsiI TTATAA 1 cut(s) 2750
Psp5II RGGWCCY 2 cut(s) 2941, 5822
PspCI CACGTG 1 cut(s) 3989
PspPPI RGGWCCY 2 cut(s) 2941, 5822
PspXI VCTCGAGB 1 cut(s) 2118
PstI CTGCAG 1 cut(s) 1684
PstNI CAGNNNCTG 2 cut(s) 482, 4471
PsuI RGATCY 7 cut(s) 226, 2397, 2476, 2577, 3096, 3553, 4422
PvuI CGATCG 1 cut(s) 3591
PvuII CAGCTG 2 cut(s) 1679, 5423
RseI CAYNNNNRTG 5 cut(s) 2078, 2440, 4248, 4878, 5531
ScaI AGTACT 3 cut(s) 362, 890, 4645
SchI GAGTC 5 cut(s) 49, 2260, 3140, 4483, 4840
SduI GDGCHC 8 cut(s) 412, 759, 1101, 2085, 2161, 2824, 5322, 5526
SexAI ACCWGGT 1 cut(s) 2895
SfaNI GCATC 8 cut(s) 40, 319, 1288, 1893, 3461, 3697, 5250, 5251
SfcI CTRYAG 8 cut(s) 364, 1170, 1680, 1891, 2583, 4486, 4701, 4942
Sfr274I CTCGAG 1 cut(s) 2118
SfuI TTCGAA 2 cut(s) 2012, 4660
SinI GGWCC 6 cut(s) 1775, 2893, 2941, 3898, 3929, 5822
SlaI CTCGAG 1 cut(s) 2118
SmiMI CAYNNNNRTG 5 cut(s) 2078, 2440, 4248, 4878, 5531
SmlI CTYRAG 3 cut(s) 1000, 2118, 4315
SmoI CTYRAG 3 cut(s) 1000, 2118, 4315
SpeI ACTAGT 2 cut(s) 2459, 4178
SphI GCATGC 1 cut(s) 1636
SseBI AGGCCT 3 cut(s) 1133, 2047, 3527
SspI AATATT 1 cut(s) 559
StuI AGGCCT 3 cut(s) 1133, 2047, 3527
StyI CCWWGG 4 cut(s) 2725, 2967, 2983, 5813
TaiI ACGT 5 cut(s) 283, 3279, 3991, 4357, 5800
TatI WGTACW 7 cut(s) 360, 888, 1514, 2175, 4643, 4887, 5059
TauI GCSGC 2 cut(s) 3619, 3851
TseFI GTSAC 5 cut(s) 1705, 2932, 3064, 4366, 4478
Tsp45I GTSAC 5 cut(s) 1705, 2932, 3064, 4366, 4478
TspGWI ACGGA 3 cut(s) 131, 3137, 4872
Van91I CCANNNNNTGG 1 cut(s) 1115
Vha464I CTTAAG 1 cut(s) 4315
VneI GTGCAC 2 cut(s) 408, 2081
VpaK11BI GGWCC 6 cut(s) 1775, 2893, 2941, 3898, 3929, 5822
VspI ATTAAT 1 cut(s) 4793
XagI CCTNNNNNAGG 1 cut(s) 1645
XapI RAATTY 9 cut(s) 2193, 2387, 2429, 2757, 3055, 3653, 4070, 4928, 5741
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 3 cut(s) 1636, 1834, 2923
XcmI CCANNNNNNNNNTGG 1 cut(s) 3295
XhoI CTCGAG 1 cut(s) 2118
XmaJI CCTAGG 1 cut(s) 5813
XmiI GTMKAC 3 cut(s) 1982, 3825, 5307
XmnI GAANNNNTTC 4 cut(s) 693, 860, 3276, 4071
ZrmI AGTACT 3 cut(s) 362, 890, 4645
Zsp2I ATGCAT 3 cut(s) 205, 1717, 1956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.