pycom07g00210

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002059
Physical Location & Seq
Forward (+)
228092 .. 245799
17708 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00210.1

Sequence Viewer

Length: 5859 bp
ATGGCATATCGAAGAGGAGGGTTTGAAGGGCAGCCGCAGAGGCGGATTCTGCGCACCCAGACTGCTGGGAGTTTTGGAGAGCCCATGCTGGATAGTGAGGTGGTGCCTTCTTCCCTCGTTGACATTGCTCCTATTCTCCGTGTTGCAAATGAAGTTGAGTCCAGGAATCCAAGAGTTGCCTATCTATGTCGGTTCTATGCCTTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGACGTGGTGTTCGCCAGTTTAAAACTGCACTTCTTCAGCGTCTAGAAAGGGAAAATGAAACAACACTGGCAGGAAGGGCAAAGAGTGATGCTCGGGAAATGCAGAGTTTTTATAGAGATTACTACAAAAAATACATTCAAGCTTTGCAGAATGCTGCTGAAAAAGTAGATCGGGCACAACTTACAAAAGCATACCAAACTGCTGCTGTTTTATTTGAGGTTTTGAAGGCTGTCAATCAGACGGAGGCTGTTGAAGTAGCTGAGGAGATTTTGGAAGCTCATACCAAGGTTGAAGAAAAACAGCAGATATATGTACCTTACAATATTCTTCCTCTTGATCCTGATAGTCAAAATCAGGCGATCATGAGATATCCCGAGATTCATGCTACTGTTTCTGCACTCCGTAACACCAGGGGTCTACCTTGGCCGAAGGGCCACAAAAAGAAAGTAGATGAAGACATTCTAGACTGGCTTCAGGCCATGTTTGGTTTTCAGAAAGATAATGTGGCAAATCAAAGGGAACACTTGATTCTGCTGGTTGCTAATGTGCACATACGGCAATTACCGAAGCCTGATCAACAACCCAAGTTGGATGACCGCGCTTTAACGGATGTGATGAAGAAGCTTTTCAAAAATTACAAAAAGTGGTGTAAATACCTGGATCGGAAAAGTAGCCTTTGGTTGCCAACTATTCAGCAGGAAGTACAGCAACGAAAATTGCTATACATGGGTTTATATCTTCTTATATGGGGAGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGCTATATCTATCATCATATGGCATTTGAATTGTATGGAATGCTAGCTGGGAGTGTTAGTCCATTGACAGGTGAGCACATAAAACCAGCCTATGGTGGTGAAGATGAGGCCTTTTTGTTGAAAGTAGTCACTCCAATATATGATACAATAGCAAAGGAGGCTAAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGACGACTTGAATGAATATTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCAGATGCTGATTTCTTTTGCCAGCCTATTGACGACATTCAAGGTGATAGAGATGAGAGAAAGAAACCATTTACTGGGGACCGATGGATTGGCAAAGTAAACTTTGTTGAAATACGCTCATTTTGGCACATTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATTGTTGCTTGGAATGGGTCGGGTGACTTAGGTTCCATGTTTGAGGGTGACGTCTTTAAGAAAGTGCTGAGTATCTTCATAACTGCTGCGATATTGAAGCTTGCACAAGCTGTTCTTGATTTAATTTTGAGCTGGAAAGCTAGGCAAAGCATGTCAGTCTTTGTCAAGCTAAGATATGTCTTGAAGGCTGTTTCAGCTGCAGCATGGGTCATTGTTTTACCAGTGACTTACGCATTTAGTTGGAAAGATCCTACTGGATTTGCACGGACCATCAAAAGTTGGTTTGGCAATGGTCCAAGTTCAAATTCTTTGTTCATTTTGGCTGTTGTCATCTACTTATCTCCAAACATACTCTCTGGATTGTTGTTTATGTTCCCATTCATTCGCCGGTATCTTGAAAGGTCAAACTTAAAGCTTGTGATGCTCATGATGTGGTGGTCTCAGACCCGGCTTTATGTTGGAAGGGGAATGCATGAAAGCTCAATTTCTCTTTTCAAGTATACCCTTTTTTGGGTTCTCCTGCTTGTGTCGAAATTAGCCTTCAGTTACTATGTAGAGATTAGGCCTCTTGTAAAACCAACTAAAGCTATAATGAAGGTTCACGTAAACACCTACCAATGGCATGAGTTCTTCCCTCAAGCCAAGAATAACATTGGTGTGGTGATTGCGCTATGGGCTCCCATTGTTCTTGTCTACTTTATGGATACTCAAATTTGGTATGCCATATTTTCCACACTATTCGGAGGTATATATGGTGCATTTCGCCGTCTCGGAGAGATTCGAACGCTGGGAATGTTGCGGTCTCGATTTGAATCACTGCCAGGTGCTTTTAATAATTTTTTGATTCCAGTGGAAAGGAATGAGCAGACTAAAAAGAAGGGAATACTAAAAGCCACTTTCTCTCGCAAGTATGATAAGAACCCATCTAGTAAAGAAAAGGAAGCGGCAAAGTTTGCACAGATGTGGAACGAAATAATTAGCAGTTTCAGAGAAGAGGATCTGATTAGTGATAGGGAAATGAACTTGTTGCTTGTTCCATATGGGGCTGACCCAGATTTAGTCGACCTAATCCAGTGGCCTCCTTTTCTGCTTGCTAGCAAGATCCCGATAGCACTGGACATGGCAAAAGACAGCAAAGATAAAGACCGTGAGCTGAAAAAGAGAATGAGTACTGACAATTATATGCGCTGTGCTATTCACGAATGCTATCTCTCGTTTAGAAGTATTATTAACTTTTTGGTCCTTGGAGATCGTGAAAAGAAGGATATAAATGACATCTTCGCCATAGTTGACGATCATATTGAAAAGGGAAACCTGACAACAGAGTTCAATATGAGTGCTCTTCCAAGTCTCCATGAACAATTTGTCAAGTTAATTGAATATCTGATGGAAAACAAAAAGGAGGACAAGGACCAGGTTGTAATTGTCTTGCTTAACATGTTAGAGGTTGTGACAAGAGACATAATGGAGGATGAAAGTCCCAACTTGCTAGATTCGAGTCATGGATCATATGGAAAAGATGAGGGAATGACACCCCTTGATCAACGGGATACATATTTTGGTGCACTGAGGTTCCCAGTCCCAGTGACCGCAAAAACAGGAGCTTGGAAAGAAAAGATCAGAAGGCTACATCTATTACTTACAGAGAAAGAGTCTGCTATGGATGTCCCATCCAACTTGGAAGCAAGAAGGCGCATTTCTTTCTTCTCCAACTCGTTGTTTATGGACATGCCTCCTGCACCCAAAGTTCGAAATATGCTTTCCTTCTCGGTTTTGACACCATACTATTCAGAAGAAGTTCTTTTCTCCAAAAAACATCTGGAATGGCAGAACGAAGATGGTGTCTCTATCCTTTTCTACTTGCAGAAGATCTTTCCAGACGAATGGACTAACTTTCTTGAGCGGGTCAAGTGTGAAAATGAAGAAGAACTCACAGAAAACGATGAATTGGAAGAGAAACTCCGTCTATGGGCATCCTATCGAGGCCAAACCTTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCTTTGGAACTTCAGGCCTTCCTTGATATGGCAAAAGATGAAGAGCTAATGGAAGGCTATAAGGCTGCTGAATCAACTATTGAGGAACGCTCAAAAGCCGAAGGGTCACTTTTGGCACAATGTCAGGCAGTGGTTGATATGAAATTCTCATATGTTGTGTCCTGCCAACAATATGGAATTCACAAGCGATCTGGAGATGCTCGTTCCAAGGACATTTTGAAACTTATGGCAACTTATCCGTCTCTTCGAGTAGCTTACATTGATGAGGTAGAGAAAACTAGTGAAGATAAGTCCAAAAAGAATGTTTGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACTAAGTCAGTTGATTCTTCTGATCCAGTTCAAAGGCTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCTGCAATATTGGGAGGGGGAAAGCCAGAAAATCAAAACCACGCCATCATTTTCACACGTGGAGAAGGCTTGCAAACCATCGATATGAACCAGGATAATTACTTGGAAGAAGCATTCAAAATGAGGAACTTACTGCAAGAATTTCTTGAGAAGCATGATGGTGTTAGATTTCCGACAATACTTGGACTTAGGGAGCATATTTTCACTGGCAGTGTTTCATCTCTTGCATGGTTTATGTCAAATCAAGAGACTTGTTTTGTAACTATTGGGCAGAGATTGCTGGCTAACCCTCTGAGAGTTAGATTCCACTATGGTCACCCAGATGTATTTGATCGACTGTTTCATCTGACTAGAGGAGGTGTCAGCAAAGCTTCCAAAGTCATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACATTACGCGAAGGTAGTGTCACTCATCATGAATATATACAAGTAGGGAAAGGACGAGATGTTGGCCTCAACCAGATCTCAATTTTTGAGGCAAAAATAGCTAATGGGAATGGCGAACAGACACTGAGTCGTGACATCTATAGGCTTGGGCATCGATTTGATTTCTTTAGAATGTTATCCTGCTATGTCACCACAATTGGTTTCTATTTCAGCACTTTATTGACTGTGCTTACGGTCTATGTGTTTCTCTACGGTCGCCTTTATCTTGTTGTCAGCGGACTTGAAAAACAGCTGAGTACTCAGCGAGGTATTCGAAACAATAAGCCTCTTCAAGTAGCTCTTGCTTCTCAGTCTCTTGTGCAAATTGGATTTTTGATGGCCTTGCCTATGGTTATGGAAATTGGTTTGGAAAAGGGGTTCCGCACTGCACTTAGCGATTTTGTATTGATGCAATTACAATTGGCCCCGGTGTTTTTCACATTTTCTCTTGGTACAAAGACCCACTATTATGGAAAGACTTTGCTTCATGGAGGTGCAGAATACAGAGGAACGGGTCGTAGCTTTGTAGTGTTCCATGCCAAATTTGCTGATAACTATAGACTCTACTCTAGAAGTCACTTTGTCAAGGGCATTGAGTTACTGATTCTGCTTGTTGTGTACCACCTATTTGGTCGTTCATATAAAAGTGGAGTTGTATATATCCTCATTACAATACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGGTTTGAGTGGCAAAAGATCGTCGATGACTGGACAGATTGGAAAAAGTGGATAAACAATCATGGAGGGATCGGTGTATCACCTGAAAAAAGTTGGGAATCCTGGTGGGAAAAAGAACATGAGCATCTTCTTTACTCTGGGGTGCGTGGTATCGTTGTTGAGATATTATTAGCATTGCGCTTTTTCATTTACCAGTATGGGCTTGTTTATCACCTTAACATCACAGACAACAAAAGTTTCCTGGTATATGGTGTTTCGTGGCTTGTTATCATTCTGATTTTGTGTCTGATGAAGGCTGTGTCTGCTGGAAGGCGACGATTAAGTGCAGATTATCAGCTTTTGTTTCGACTGGTTAAGGGATTTATATTTATTACATTTTTAGCTATATTCGTTACCTTAATAGTGCTCCCTCATATGACGCTTCGGGATGTCGTAGTGTGCATTCTTGCCTTCATGCCAACTGGTTGGGGATTGCTTTTGATTGCGCAAGCTTGTAAGCCGGTAATTAAACGAGCAGGGTTTTGGGAATCAGTTCAAACACTTGCTCGTGGTTATGAAATAATCATGGGGTTGCTTCTTTTTACCCCGGTTGCTTTCTTGGCTTGGTTTCCGTTTGTTTCCGAATTCCAGACGCGAATGCTTTTCAACCAAGCATTCAGTAGAGGTTTACAGATCTCGCGTATTCTTGGAGGACAAAAGAAGGGCCCTCACTCCTCCAACAATAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1953

Amino Acids

225.12

Weight (kDa)

9.08

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 169 2.3e-17 Vta1 like
FKS1_dom1 PF14288 318 - 430 1.3e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 855 - 1043 5.1e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1051 - 1143 2.1e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1143 - 1760 2.1e-205 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1539
Acc16I TGCGCA 2 cut(s) 53, 5613
Acc36I ACCTGC 2 cut(s) 3946, 4334
AccB1I GGYRCC 1 cut(s) 103
AccB7I CCANNNNNTGG 2 cut(s) 1118, 1370
AccBSI CCGCTC 1 cut(s) 3388
AccI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
AccII CGCG 4 cut(s) 837, 4365, 5761, 5806
AclWI GGATC 9 cut(s) 218, 569, 906, 1727, 2490, 2581, 2998, 3881, 5204
AcoI YGGCCR 1 cut(s) 662
AcsI RAATTY 8 cut(s) 1000, 1789, 2196, 3659, 3693, 4076, 4937, 5750
AcuI CTGAAG 4 cut(s) 257, 695, 2011, 3512
AcvI CACGTG 1 cut(s) 3995
AcyI GRCGYC 1 cut(s) 1536
AfaI GTAC 7 cut(s) 552, 942, 2656, 3492, 4654, 4849, 5015
AfiI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
AflII CTTAAG 1 cut(s) 4324
AflIII ACRYGT 2 cut(s) 2922, 3992
AhdI GACNNNNNGTC 1 cut(s) 4482
AhlI ACTAGT 1 cut(s) 3794
AjiI CACGTC 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 1774, 1806
AleI CACNNNNGTG 1 cut(s) 2446
AloI GAACNNNNNNTCC 4 cut(s) 231, 263, 4757, 4789
Alw21I GWGCWC 5 cut(s) 789, 1104, 2827, 3052, 5535
Alw26I GTCTC 9 cut(s) 1929, 2258, 2292, 2840, 2937, 3334, 3762, 4178, 4713
Alw44I GTGCAC 2 cut(s) 785, 3048
AlwI GGATC 9 cut(s) 218, 569, 906, 1727, 2490, 2581, 2998, 3881, 5204
AlwNI CAGNNNCTG 2 cut(s) 1304, 4480
Ama87I CYCGRG 2 cut(s) 330, 611
ApaI GGGCCC 1 cut(s) 5834
ApaLI GTGCAC 2 cut(s) 785, 3048
ApeKI GCWGC 8 cut(s) 31, 392, 440, 995, 1571, 1682, 1685, 3581
ApoI RAATTY 8 cut(s) 1000, 1789, 2196, 3659, 3693, 4076, 4937, 5750
ArsI GACNNNNNNTTYG 6 cut(s) 231, 263, 3312, 3344, 4359, 4391
Asp700I GAANNNNTTC 4 cut(s) 696, 863, 3282, 5658
AspLEI GCGC 7 cut(s) 54, 839, 2155, 2673, 3180, 5307, 5614
AsuC2I CCSGG 3 cut(s) 1933, 4823, 5714
AsuII TTCGAA 3 cut(s) 2266, 3235, 4669
AsuNHI GCTAGC 2 cut(s) 1069, 2579
AvaI CYCGRG 2 cut(s) 330, 611
AvaII GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
BaeGI GKGCMC 4 cut(s) 415, 789, 3052, 5834
BanI GGYRCC 1 cut(s) 103
BanII GRGCYC 3 cut(s) 84, 2164, 5834
BauI CACGAG 1 cut(s) 5673
BbrPI CACGTG 1 cut(s) 3995
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 5 cut(s) 789, 1104, 2827, 3052, 5535
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 8 cut(s) 43, 379, 427, 982, 1558, 1669, 1697, 3568
BceAI ACGGC 2 cut(s) 809, 2235
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciVI GTATCC 2 cut(s) 2182, 3028
BclI TGATCA 3 cut(s) 811, 1482, 3025
BcnI CCSGG 3 cut(s) 1933, 4823, 5714
BcoDI GTCTC 9 cut(s) 1929, 2258, 2292, 2840, 2937, 3334, 3762, 4178, 4713
BcuI ACTAGT 1 cut(s) 3794
BfmI CTRYAG 3 cut(s) 1683, 4495, 4951
BfrI CTTAAG 1 cut(s) 4324
BfuAI ACCTGC 2 cut(s) 3946, 4334
BfuI GTATCC 2 cut(s) 2182, 3028
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 3 cut(s) 3354, 4431, 5799
BmcAI AGTACT 2 cut(s) 2656, 4654
Bme18I GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
BmeRI GACNNNNNGTC 1 cut(s) 4482
BmeT110I CYCGRG 2 cut(s) 330, 611
BmgBI CACGTC 1 cut(s) 242
BmiI GGNNCC 9 cut(s) 105, 1376, 1519, 2163, 3059, 3858, 4775, 4821, 5832
BmrI ACTGGG 3 cut(s) 1380, 3056, 3062
BmsI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5260
BmtI GCTAGC 2 cut(s) 1073, 2583
BmuI ACTGGG 3 cut(s) 1380, 3056, 3062
BpiI GAAGAC 1 cut(s) 699
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 3590, 3622
BpmI CTGGAG 1 cut(s) 3729
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 3 cut(s) 2266, 3235, 4669
BpuEI CTTGAG 3 cut(s) 2106, 3404, 4103
BpuMI CCSGG 3 cut(s) 1933, 4823, 5714
Bsa29I ATCGAT 2 cut(s) 4017, 4510
BsaAI YACGTR 2 cut(s) 2089, 3995
BsaBI GATNNNNATC 2 cut(s) 2296, 3915
BsaHI GRCGYC 1 cut(s) 1536
BsaI GGTCTC 2 cut(s) 1929, 2292
BsaJI CCNNGG 7 cut(s) 522, 648, 659, 2728, 3723, 4821, 5712
BsaWI WCCGGW 1 cut(s) 3510
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
Bse118I RCCGGY 2 cut(s) 1872, 5626
Bse3DI GCAATG 4 cut(s) 123, 1485, 1780, 5300
Bse8I GATNNNNATC 2 cut(s) 2296, 3915
BseCI ATCGAT 2 cut(s) 4017, 4510
BseDI CCNNGG 7 cut(s) 522, 648, 659, 2728, 3723, 4821, 5712
BseGI GGATG 7 cut(s) 835, 853, 2962, 3154, 3155, 3458, 5560
BseJI GATNNNNATC 2 cut(s) 2296, 3915
BseLI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
BseMI GCAATG 4 cut(s) 123, 1485, 1780, 5300
BseMII CTCAG 9 cut(s) 489, 1544, 1940, 3044, 4220, 4472, 4640, 4670, 4718
BseRI GAGGAG 5 cut(s) 30, 515, 3849, 4305, 5830
BseSI GKGCMC 4 cut(s) 415, 789, 3052, 5834
BseXI GCAGC 8 cut(s) 43, 379, 427, 982, 1558, 1669, 1697, 3568
BseYI CCCAGC 3 cut(s) 65, 1073, 2272
BsgI GTGCAG 7 cut(s) 249, 618, 1317, 3207, 4767, 4911, 5472
Bsh1236I CGCG 4 cut(s) 837, 4365, 5761, 5806
Bsh1285I CGRYCG 1 cut(s) 4612
BshNI GGYRCC 1 cut(s) 103
BshVI ATCGAT 2 cut(s) 4017, 4510
BsiEI CGRYCG 1 cut(s) 4612
BsiHKAI GWGCWC 5 cut(s) 789, 1104, 2827, 3052, 5535
BsiHKCI CYCGRG 2 cut(s) 330, 611
BsiSI CCGG 6 cut(s) 1873, 1933, 3511, 4823, 5627, 5714
BslFI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
BslI CCNNNNNNNGG 8 cut(s) 1094, 1118, 1370, 1995, 1996, 2103, 3454, 3544
BsmAI GTCTC 9 cut(s) 1929, 2258, 2292, 2840, 2937, 3334, 3762, 4178, 4713
BsmBI CGTCTC 2 cut(s) 2258, 3762
BsmFI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
Bso31I GGTCTC 2 cut(s) 1929, 2292
BsoBI CYCGRG 2 cut(s) 330, 611
Bsp119I TTCGAA 3 cut(s) 2266, 3235, 4669
Bsp120I GGGCCC 1 cut(s) 5830
Bsp1286I GDGCHC 9 cut(s) 84, 415, 789, 1104, 2164, 2827, 3052, 5535, 5834
BspCNI CTCAG 9 cut(s) 490, 1545, 1939, 3045, 4221, 4473, 4641, 4669, 4717
BspDI ATCGAT 2 cut(s) 4017, 4510
BspFNI CGCG 4 cut(s) 837, 4365, 5761, 5806
BspHI TCATGA 3 cut(s) 600, 1911, 4384
BspLI GGNNCC 9 cut(s) 105, 1376, 1519, 2163, 3059, 3858, 4775, 4821, 5832
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 2 cut(s) 3946, 4334
BspOI GCTAGC 2 cut(s) 1073, 2583
BspPI GGATC 9 cut(s) 218, 569, 906, 1727, 2490, 2581, 2998, 3881, 5204
BspQI GCTCTTC 2 cut(s) 2832, 3552
BspT104I TTCGAA 3 cut(s) 2266, 3235, 4669
BspT107I GGYRCC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 4324
BspTNI GGTCTC 2 cut(s) 1929, 2292
BsrBI CCGCTC 1 cut(s) 3388
BsrDI GCAATG 4 cut(s) 123, 1485, 1780, 5300
BsrFI RCCGGY 2 cut(s) 1872, 5626
BssAI RCCGGY 2 cut(s) 1872, 5626
BssECI CCNNGG 7 cut(s) 522, 648, 659, 2728, 3723, 4821, 5712
BssNAI GTATAC 1 cut(s) 1986
BssNI GRCGYC 1 cut(s) 1536
BssSI CACGAG 1 cut(s) 5673
BssT1I CCWWGG 4 cut(s) 522, 659, 2728, 3723
Bst1107I GTATAC 1 cut(s) 1986
Bst2BI CACGAG 1 cut(s) 5673
Bst4CI ACNGT 7 cut(s) 628, 2633, 3490, 4275, 4582, 4591, 4610
Bst6I CTCTTC 8 cut(s) 7, 2472, 2832, 3432, 3552, 3765, 4689, 5120
BstACI GRCGYC 1 cut(s) 1536
BstAFI CTTAAG 1 cut(s) 4324
BstAPI GCANNNNNTGC 2 cut(s) 2438, 4213
BstBAI YACGTR 2 cut(s) 2089, 3995
BstBI TTCGAA 3 cut(s) 2266, 3235, 4669
BstEII GGTNACC 1 cut(s) 4250
BstF5I GGATG 7 cut(s) 835, 853, 2962, 3154, 3155, 3458, 5560
BstFNI CGCG 4 cut(s) 837, 4365, 5761, 5806
BstHHI GCGC 7 cut(s) 54, 839, 2155, 2673, 3180, 5307, 5614
BstMAI GTCTC 9 cut(s) 1929, 2258, 2292, 2840, 2937, 3334, 3762, 4178, 4713
BstMCI CGRYCG 1 cut(s) 4612
BstNSI RCATGY 3 cut(s) 1639, 2926, 3217
BstPI GGTNACC 1 cut(s) 4250
BstSFI CTRYAG 3 cut(s) 1683, 4495, 4951
BstSLI GKGCMC 4 cut(s) 415, 789, 3052, 5834
BstUI CGCG 4 cut(s) 837, 4365, 5761, 5806
BstV1I GCAGC 8 cut(s) 43, 379, 427, 982, 1558, 1669, 1697, 3568
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 7 cut(s) 223, 1732, 2482, 2586, 3354, 4431, 5799
BstXI CCANNNNNNTGG 6 cut(s) 65, 3369, 3689, 4865, 5024, 5592
BstYI RGATCY 7 cut(s) 223, 1732, 2482, 2586, 3354, 4431, 5799
BstZ17I GTATAC 1 cut(s) 1986
Bsu15I ATCGAT 2 cut(s) 4017, 4510
BsuI GTATCC 2 cut(s) 2182, 3028
BsuTUI ATCGAT 2 cut(s) 4017, 4510
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 7 cut(s) 835, 853, 2962, 3154, 3155, 3458, 5560
BtsI GCAGTG 4 cut(s) 2300, 3651, 4153, 4779
BveI ACCTGC 2 cut(s) 3946, 4334
CaiI CAGNNNCTG 2 cut(s) 1304, 4480
CciI TCATGA 3 cut(s) 600, 1911, 4384
CfoI GCGC 7 cut(s) 54, 839, 2155, 2673, 3180, 5307, 5614
Cfr10I RCCGGY 2 cut(s) 1872, 5626
ClaI ATCGAT 2 cut(s) 4017, 4510
CseI GACGC 3 cut(s) 266, 5554, 5767
CsiI ACCWGGT 1 cut(s) 2898
Csp6I GTAC 7 cut(s) 551, 941, 2655, 3491, 4653, 4848, 5014
CviQI GTAC 7 cut(s) 551, 941, 2655, 3491, 4653, 4848, 5014
DraI TTTAAA 1 cut(s) 259
DriI GACNNNNNGTC 1 cut(s) 4482
EaeI YGGCCR 1 cut(s) 662
Eam1104I CTCTTC 8 cut(s) 7, 2472, 2832, 3432, 3552, 3765, 4689, 5120
Eam1105I GACNNNNNGTC 1 cut(s) 4482
EarI CTCTTC 8 cut(s) 7, 2472, 2832, 3432, 3552, 3765, 4689, 5120
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 4 cut(s) 522, 659, 2728, 3723
Eco147I AGGCCT 3 cut(s) 1136, 2050, 3533
Eco24I GRGCYC 3 cut(s) 84, 2164, 5834
Eco31I GGTCTC 2 cut(s) 1929, 2292
Eco32I GATATC 1 cut(s) 608
Eco47I GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
Eco57I CTGAAG 4 cut(s) 257, 695, 2011, 3512
Eco72I CACGTG 1 cut(s) 3995
Eco88I CYCGRG 2 cut(s) 330, 611
Eco91I GGTNACC 1 cut(s) 4250
EcoO109I RGGNCCY 2 cut(s) 5830, 5831
EcoO65I GGTNACC 1 cut(s) 4250
EcoRI GAATTC 2 cut(s) 3693, 5750
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 4 cut(s) 522, 659, 2728, 3723
EcoT22I ATGCAT 1 cut(s) 1959
EcoT38I GRGCYC 3 cut(s) 84, 2164, 5834
ErhI CCWWGG 4 cut(s) 522, 659, 2728, 3723
Esp3I CGTCTC 2 cut(s) 2258, 3762
FaqI GGGAC 4 cut(s) 1388, 2949, 3050, 3137
FauI CCCGC 1 cut(s) 3381
FauNDI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5541
FbaI TGATCA 3 cut(s) 811, 1482, 3025
FblI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
FokI GGATG 7 cut(s) 842, 860, 2969, 3142, 3161, 3445, 5567
FriOI GRGCYC 3 cut(s) 84, 2164, 5834
FspI TGCGCA 2 cut(s) 53, 5613
GlaI GCGC 7 cut(s) 53, 838, 2154, 2672, 3179, 5306, 5613
GsaI CCCAGC 3 cut(s) 69, 1077, 2276
GsuI CTGGAG 1 cut(s) 3729
HapII CCGG 6 cut(s) 1873, 1933, 3511, 4823, 5627, 5714
HgaI GACGC 3 cut(s) 266, 5554, 5767
HhaI GCGC 7 cut(s) 54, 839, 2155, 2673, 3180, 5307, 5614
Hin1I GRCGYC 1 cut(s) 1536
Hin6I GCGC 7 cut(s) 52, 837, 2153, 2671, 3178, 5305, 5612
HinP1I GCGC 7 cut(s) 52, 837, 2153, 2671, 3178, 5305, 5612
HincII GTYRAC 3 cut(s) 121, 2548, 2776
HindII GTYRAC 3 cut(s) 121, 2548, 2776
HindIII AAGCTT 8 cut(s) 378, 860, 1583, 1898, 3516, 3926, 4305, 5616
HpaII CCGG 6 cut(s) 1873, 1933, 3511, 4823, 5627, 5714
Hpy99I CGWCG 2 cut(s) 5153, 5445
HpyCH4III ACNGT 7 cut(s) 628, 2633, 3490, 4275, 4582, 4591, 4610
HpyCH4IV ACGT 4 cut(s) 241, 1536, 2088, 3994
HpySE526I ACGT 4 cut(s) 241, 1536, 2088, 3994
Hsp92I GRCGYC 1 cut(s) 1536
HspAI GCGC 7 cut(s) 52, 837, 2153, 2671, 3178, 5305, 5612
Ksp22I TGATCA 3 cut(s) 811, 1482, 3025
LguI GCTCTTC 2 cut(s) 2832, 3552
LmnI GCTCC 9 cut(s) 133, 1194, 1452, 2167, 3086, 3862, 4129, 5110, 5538
Lsp1109I GCAGC 8 cut(s) 43, 379, 427, 982, 1558, 1669, 1697, 3568
LweI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5260
MabI ACCWGGT 1 cut(s) 2898
MaeII ACGT 4 cut(s) 241, 1536, 2088, 3994
MbiI CCGCTC 1 cut(s) 3388
MfeI CAATTG 2 cut(s) 4551, 4814
MflI RGATCY 7 cut(s) 223, 1732, 2482, 2586, 3354, 4431, 5799
MhlI GDGCHC 9 cut(s) 84, 415, 789, 1104, 2164, 2827, 3052, 5535, 5834
MlyI GAGTC 5 cut(s) 167, 2992, 3146, 4492, 4950
MmeI TCCRAC 8 cut(s) 807, 1706, 1924, 3183, 3219, 3887, 4133, 5068
Mph1103I ATGCAT 1 cut(s) 1959
MroXI GAANNNNTTC 4 cut(s) 696, 863, 3282, 5658
MslI CAYNNNNRTG 7 cut(s) 2141, 2446, 4019, 4095, 4257, 4863, 4887
MspA1I CMGCKG 3 cut(s) 1682, 4632, 4648
MspCI CTTAAG 1 cut(s) 4324
MspI CCGG 6 cut(s) 1873, 1933, 3511, 4823, 5627, 5714
MunI CAATTG 2 cut(s) 4551, 4814
MvnI CGCG 4 cut(s) 837, 4365, 5761, 5806
NciI CCSGG 3 cut(s) 1933, 4823, 5714
NdeI CATATG 5 cut(s) 1044, 2524, 2995, 3667, 5541
NheI GCTAGC 2 cut(s) 1069, 2579
NlaIV GGNNCC 9 cut(s) 105, 1376, 1519, 2163, 3059, 3858, 4775, 4821, 5832
NsbI TGCGCA 2 cut(s) 53, 5613
NsiI ATGCAT 1 cut(s) 1959
NspI RCATGY 3 cut(s) 1639, 2926, 3217
NspV TTCGAA 3 cut(s) 2266, 3235, 4669
OliI CACNNNNGTG 1 cut(s) 2446
PagI TCATGA 3 cut(s) 600, 1911, 4384
PceI AGGCCT 3 cut(s) 1136, 2050, 3533
PciI ACATGT 1 cut(s) 2922
PciSI GCTCTTC 2 cut(s) 2832, 3552
PdmI GAANNNNTTC 4 cut(s) 696, 863, 3282, 5658
PflMI CCANNNNNTGG 2 cut(s) 1118, 1370
PfoI TCCNGGA 2 cut(s) 161, 3931
PleI GAGTC 5 cut(s) 166, 2991, 3145, 4491, 4950
PmaCI CACGTG 1 cut(s) 3995
PmlI CACGTG 1 cut(s) 3995
PpsI GAGTC 5 cut(s) 166, 2991, 3145, 4491, 4950
Ppu21I YACGTR 2 cut(s) 2089, 3995
PscI ACATGT 1 cut(s) 2922
PspCI CACGTG 1 cut(s) 3995
PspEI GGTNACC 1 cut(s) 4250
PspFI CCCAGC 3 cut(s) 65, 1073, 2272
PspN4I GGNNCC 9 cut(s) 105, 1376, 1519, 2163, 3059, 3858, 4775, 4821, 5832
PspOMI GGGCCC 1 cut(s) 5830
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 2 cut(s) 1304, 4480
PsuI RGATCY 7 cut(s) 223, 1732, 2482, 2586, 3354, 4431, 5799
PvuII CAGCTG 2 cut(s) 1682, 4648
RsaI GTAC 7 cut(s) 552, 942, 2656, 3492, 4654, 4849, 5015
RsaNI GTAC 7 cut(s) 551, 941, 2655, 3491, 4653, 4848, 5014
RseI CAYNNNNRTG 7 cut(s) 2141, 2446, 4019, 4095, 4257, 4863, 4887
SalI GTCGAC 1 cut(s) 2546
SapI GCTCTTC 2 cut(s) 2832, 3552
ScaI AGTACT 2 cut(s) 2656, 4654
SchI GAGTC 5 cut(s) 167, 2992, 3146, 4492, 4950
SduI GDGCHC 9 cut(s) 84, 415, 789, 1104, 2164, 2827, 3052, 5535, 5834
SexAI ACCWGGT 1 cut(s) 2898
SfaNI GCATC 8 cut(s) 316, 1291, 1896, 3467, 3703, 4516, 4794, 5260
SfcI CTRYAG 3 cut(s) 1683, 4495, 4951
SfuI TTCGAA 3 cut(s) 2266, 3235, 4669
SinI GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
SmiMI CAYNNNNRTG 7 cut(s) 2141, 2446, 4019, 4095, 4257, 4863, 4887
SmlI CTYRAG 4 cut(s) 2121, 3383, 4082, 4324
SmoI CTYRAG 4 cut(s) 2121, 3383, 4082, 4324
SpeI ACTAGT 1 cut(s) 3794
SseBI AGGCCT 3 cut(s) 1136, 2050, 3533
SspI AATATT 3 cut(s) 562, 1253, 3945
StuI AGGCCT 3 cut(s) 1136, 2050, 3533
StyI CCWWGG 4 cut(s) 522, 659, 2728, 3723
TaaI ACNGT 7 cut(s) 628, 2633, 3490, 4275, 4582, 4591, 4610
TaiI ACGT 4 cut(s) 244, 1539, 2091, 3997
TaqII GACCGA 1 cut(s) 1392
TatI WGTACW 3 cut(s) 940, 2654, 4652
TauI GCSGC 3 cut(s) 37, 2432, 3857
TseI GCWGC 8 cut(s) 31, 392, 440, 995, 1571, 1682, 1685, 3581
TspGWI ACGGA 8 cut(s) 128, 494, 629, 860, 1765, 3437, 3744, 5727
Van91I CCANNNNNTGG 2 cut(s) 1118, 1370
Vha464I CTTAAG 1 cut(s) 4324
VneI GTGCAC 2 cut(s) 785, 3048
VpaK11BI GGWCC 7 cut(s) 1375, 1752, 1778, 2725, 2896, 3904, 3935
XapI RAATTY 8 cut(s) 1000, 1789, 2196, 3659, 3693, 4076, 4937, 5750
XbaI TCTAGA 3 cut(s) 280, 700, 4964
XceI RCATGY 3 cut(s) 1639, 2926, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3301
XmiI GTMKAC 5 cut(s) 655, 1985, 2178, 2547, 3831
XmnI GAANNNNTTC 4 cut(s) 696, 863, 3282, 5658
ZraI GACGTC 1 cut(s) 1537
ZrmI AGTACT 2 cut(s) 2656, 4654
Zsp2I ATGCAT 1 cut(s) 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.