AT2G31960

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002059
Physical Location & Seq
Forward (+)
13588522 .. 13600430
11909 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31960.5

Sequence Viewer

Length: 5853 bp
ATGGCTCAAAGGAAAGGACCTGATCCGCCTCCGCCTCAAAGGCGGATTCTGCGGACTCAAACTGCTGGTAATCTCGGAGAGGCTATGTTGGACAGTGAAGTGGTGCCATCGTCTCTTGTGGAGATTGCTCCGATCCTTCGTGTAGCTAATGAAGTTGAAGCTAGTAACCCCCGTGTTGCTTACTTATGTCGGTTTTATGCGTTTGAGAAGGCACACAGGCTTGATCCCACATCAAGTGGACGTGGTGTTCGCCAGTTTAAGACTGCGCTTCTTCAACGGTTAGAACGGGAGAATGAAACAACTCTTGCAGGAAGGCAGAAGAGTGATGCACGCGAAATGCAAAGTTTCTATCAACACTACTACAAGAAATACATCCAAGCTTTGCAAAACGCCGCTGACAAAGCTGACCGTGCTCAACTTACAAAGGCATATCAAACTGCTGCTGTTCTCTTTGAAGTCTTGAAAGCTGTTAATCAGACAGAAGATGTGGAAGTTGCTGATGAGATTTTGGAGGCTCACACAAAGGTGGAAGAAAAAAGCCAAATCTACGTGCCTTACAACATTCTTCCCCTTGATCCTGATAGTCAAAATCAGGCTATAATGAGATTTCCTGAGATACAAGCTACAGTAAGCGCACTTCGTAACACTAGGGGTTTACCATGGCCAGCTGGTCACAAGAAGAAACTCGATGAAGACATGCTAGACTGGCTTCAAACTATGTTTGGTTTTCAGAAAGATAATGTTTCAAATCAAAGAGAGCACTTGATTTTGTTACTTGCTAATGTCCACATTCGACAGTTTCCAAGACCTGAACAACAACCAAGGCTGGATGATCGTGCATTGACTATAGTGATGAAGAAACTTTTCAAAAACTACAAAAAGTGGTGCAAGTATTTGGGTCGGAAGAGCAGCCTTTGGTTGCCGACGATTCAACAAGAGGTTCAGCAGCGGAAACTGCTATACATGGGACTCTATCTTTTAATATGGGGAGAAGCTGCAAACCTGAGATTTTTGCCAGAATGCCTTTGCTATATATATCACCATATGGCTTTTGAGCTGTATGGGATGTTGGCTGGGAGTGTTAGTCCAATGACAGGGGAGCATGTAAAACCGGCATATGGTGGTGAAGACGAAGCTTTCCTGCAAAAAGTAGTGACTCCTATATACAAGACAATAGCAAAGGAAGCAAAGAGAAGTAGAGGAGGAAAATCAAAGCATTCTGAGTGGAGAAACTATGACGACTTGAATGAATATTTTTGGTCAATTCGGTGCTTTCGGTTAGGGTGGCCTATGCGAGCTGATGCTGATTTCTTTTGTCAGACAGCGGAAGAACTTCGACTTGACAGAAGTGAGAATAAACCAAAAACTGGAGATCGATGGATGGGCAAAGTCAATTTTGTTGAGATACGCTCCTTCTGGCATATATTTAGAAGTTTCGATAGAATGTGGAGTTTCTATATCCTGTCTTTGCAGGCAATGATCATAATTGCTTGGAATGGATCTGGGAAATTAAGCGGCATCTTCCAAGGTGATGTCTTCCTAAAGGTTTTGAGCATCTTCATTACGGCAGCTATATTAAAGCTTGCACAAGCTGTCCTTGATATAGCTCTAAGCTGGAAATCAAGACATAGTATGTCATTCCATGTGAAGCTCAGATTTATCTTCAAGGCAGTTGCAGCTGCAATCTGGGTGGTTCTCATGCCACTAACTTACGCATACAGCTGGAAGACCCCTTCTGGTTTTGCAGAGACCATAAAGAACTGGTTTGGGGGACACCAAAATTCCTCACCTTCCTTTTTCATTATTGTCATTCTTATCTACTTATCCCCAAATATGCTCTCTACATTGCTCTTCGCGTTCCCATTCATCCGACGTTATCTTGAAAGGTCTGACTATAAAATAGTGATGCTTATGATGTGGTGGTCTCAGCCGAGACTCTATATAGGGAGGGGTATGCATGAGAGTGCATTGTCACTTTTCAAGTACACGATGTTCTGGGTTGTACTTCTAATATCAAAGCTTGCATTCAGTTTTTATGCAGAGATTAAGCCTCTTGTTAAACCAACCAAAGACATTATGCGAGTTCATATATCAGTCTACCGCTGGCATGAGTTTTTCCCTCACGCCAAGAGTAATATGGGTGTGGTGATCGCTCTCTGGTCACCTGTTATTCTTGTTTATTTCATGGACACTCAAATATGGTATGCTATAGTTTCAACTCTGGTGGGAGGTTTAAATGGAGCTTTTCGACGTCTTGGAGAGATCCGAACTCTAGGAATGTTGAGGTCTAGATTTCAGTCCTTACCAGAGGCCTTTAATGCTTGCTTGGTTCCAAATGAGAAGAGCGAAACTCCAAAGAAAAAGGGGATTATGGCGACCTTCACTCGCAAGTTCGATCAGGTTCCATCTAGTAAAGACAAGGAGGCCGCACGATTTGCTCAGATGTGGAACAAAATAATCAGTAGTTTCCGAGAGGAGGATTTGATTAGCGATAGGGAAATGGAACTCCTGCTTGTGCCATATTGGGCTGACCGTGATCTGGATCTTATCCGCTGGCCACCTTTTCTGCTGGCTAGTAAGATCCCAATAGCTTTGGATATGGCAAAAGATAGCAATGGAAAAGATCGTGAACTGACCAAGAGATTGAGTGTCGACAGTTACATGACTTGTGCGGTTAGAGAGTGCTACGCCTCTTTTAAAAATCTCATAAACTTTTTGGTTGTTGGAGAACGAGAAGGACAGGTCATAAACGAAATCTTCTCCAGGATTGATGAACATATAGAGAAAGAAACACTAATAAAGGACTTGAACTTGAGTGCTCTTCCCGATCTGTATGGTCAATTTGTTCGACTAATTGAATATCTGATGGAAAACAGAGAGGAGGACAAGGACCAAATTGTTATTGTCTTGCTGAACATGTTAGAAGTGGTGACAAGAGACATAATGGACGAAGAGGTCCCAAGCATGCTAGAGTCAACTCACAATGGAACTTATGTCAAGTACGACGTAATGACTCCTCTTCATCAACAGCGCAAATATTTCAGCCAACTTCGATTCCCAGTGTACTCTCAGACAGAAGCCTGGAAAGAAAAAATCAAGAGGCTTCATCTTTTACTTACTGTCAAAGAGTCGGCTATGGATGTGCCATCCAACTTGGAAGCTCGAAGGCGACTTACATTCTTTTCAAATTCATTATTCATGGAAATGCCTGATGCCCCCAAAATCCGCAACATGCTTTCCTTTTCGGTCCTAACACCATACTATTCAGAGGATGTTCTATTCTCCATTTTTGGTTTGGAGAAGCAAAATGAAGATGGAGTCTCTATACTCTTTTACTTGCAGAAGATCTTTCCAGATGAGTGGACAAACTTTTTGGAACGCGTGAAATGTGGCAGCGAAGAAGAACTCAGAGCAAGAGAAGAGTTGGAAGAGGAACTTCGTCTGTGGGCATCTTACAGAGGCCAAACACTGACCAAAACTGTGCGAGGCATGATGTATTACCGAAAGGCTTTGGAGCTCCAGGCCTTCCTTGATATGGCAAAGGATGAAGAATTAATGAAAGGGTACAAGGCTCTCGAGTTAACTAGTGAAGACGCATCGAAGAGTGGAACGTCGCTCTGGGCTCAGTGTCAAGCACTTGCTGACATGAAATTTACCTTTGTGGTTTCATGCCAACAATACAGTGTTCAGAAGAGATCCGGTGACCAACGTGCGAAGGACATATTGAGGCTTATGACTACGTACCCGTCTCTACGTGTTGCTTATATTGACGAGGTTGAGCAAACACATAAAGAAAGCTATAAAGGGGCAGACGAAAAAATTTACTACTCAGCCCTTGTCAAAGCTGCTCCGCAGACCAAATCTATGGATTCTTCTGAATCTGTGCAAACACTAGACCAGGTCATATACCGGATTAAGCTACCAGGACCTGCAATACTAGGAGAGGGGAAGCCAGAGAATCAGAACCATTCTATCATTTTTACTCGCGGAGAAGGGTTACAGACAATAGACATGAACCAGGACAATTACATGGAGGAAGCTTTCAAAATGAGGAACTTGCTTCAAGAATTTCTTGTTAAGCATGGAGGCGTAAGAACCCCTACAATACTTGGACTTAGAGAGCATATTTTCACGGGAAGTGTTTCTTCTCTTGCATGGTTTATGTCGAATCAGGAAAACAGCTTTGTTACTATTGGGCAGAGGGTGCTAGCTAGCCCCTTGAAGGTACGATTCCATTACGGTCATCCAGATGTTTTTGATCGACTGTTTCACCTTACCAGAGGTGGTGTCTGCAAAGCTTCTAAAGTTATCAATCTAAGTGAAGACATATTTGCTGGTTTCAACTCTACTCTTCGTGAAGGCAATGTGACTCATCATGAATACATACAAGTTGGTAAAGGGAGAGATGTAGGACTCAACCAGATTTCAATGTTTGAGGCAAAGATCGCTAACGGAAATGGGGAGCAAACTCTAAGCCGTGACCTATATAGGCTGGGGCACCGATTTGATTTCTTCCGCATGCTTTCTTGTTATTTCACTACAATTGGGTTCTACTTCAGTACTATGTTAACCGTGCTTACGGTCTATGTATTTCTATATGGACGGCTGTATCTCGTTCTTAGTGGTCTTGAAGAAGGATTGAGCAACCAGAAAGCTTTTCGATCCAACATGCCTCTTCAAGCTGCACTTGCTTCTCAATCATTTGTGCAGATTGGTTTCTTGATGGCCTTACCTATGATGATGGAGATAGGTCTTGAGAGGGGATTCCATAACGCCCTAATTGATTTTGTACTGATGCAGTTACAACTCGCTTCCGTGTTTTTCACGTTCCAACTGGGGACAAAAACTCATTACTATGGTCGAACATTGTTTCATGGCGGCGCAGAATACAGAGGCACTGGGCGTGGTTTTGTGGTGTTCCATGCCAAATTTGCTGAAAACTACAGATTCTACTCGCGCAGTCATTTTGTGAAGGGTATTGAGCTGATGATACTCCTGCTAGTGTATCAGATATTTGGCCATGCCTATAGAGGAGTTGTTACATATATTCTGATCACTGTTTCGATATGGTTCATGGTGGTGACTTGGCTCTTTGCTCCTTTCCTGTTCAATCCCTCTGGTTTCGAGTGGCAAAAGATCGTAGATGATTGGACAGATTGGAATAAATGGATATATAACCGAGGGGGAATTGGTGTTCCTCCAGAAAAGAGTTGGGAGTCTTGGTGGGAGAAGGAAATAGGACATCTTCGACACTCGGGAAAACGGGGTATCATCCTCGAGATTGTTTTGGCTTTACGCTTCTTCATCTTTCAGTACGGACTGGTTTATCAACTCAGCACCTTTAAACAGGAAAATCAAAGTTTGTGGATTTATGGAGCTTCATGGTTCGTGATCTTATTCATTTTACTTATTGTAAAGGGTCTGGGTGTGGGAAGACAACGGTTCAGCACAAACTTCCAGCTTCTCTTCAGGATCATCAAGGGTTTTGTGTTCCTCACATTTCTTGGACTTCTCATTACTTTCTTAGCTCTTCGTTTTTTAACACCCAAGGACATCTTCCTTTGTATGCTCGCTTTCATGCCAACCGGTTGGGGAATGTTACTCATTGCACAAGCGTGTAAGCCTCTGATTCAACGGCTAGGATTCTGGTCATCGGTTAGGACGCTGGCTCGAGGCTATGAGATTCTCATGGGTTTACTTTTGTTCACTCCGGTAGCGTTCCTGGCTTGGTTCCCGTTCGTGTCTGAGTTTCAGACAAGAATGCTCTTTAACCAAGCATTTAGCAGAGGATTGCAGATTTCACGTATTCTTGGTGGTCAGAGAAAAGACCGCTCTTCCAAGAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1950

Amino Acids

226.02

Weight (kDa)

9.21

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 42 - 169 5.7e-18 Vta1 like
FKS1_dom1 PF14288 318 - 430 4.8e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 853 - 1041 2.2e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1048 - 1138 1.4e-27 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1139 - 1758 2.7e-207 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2954
AatII GACGTC 1 cut(s) 2254
Acc36I ACCTGC 1 cut(s) 3937
AccB1I GGYRCC 2 cut(s) 103, 4497
AccB7I CCANNNNNTGG 1 cut(s) 1367
AccBSI CCGCTC 1 cut(s) 5832
AccI GTMKAC 2 cut(s) 2097, 2652
AccII CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
AcoI YGGCCR 3 cut(s) 662, 2555, 5017
AcsI RAATTY 6 cut(s) 1780, 3187, 3650, 3819, 4067, 4928
AcuI CTGAAG 2 cut(s) 4540, 5452
AcyI GRCGYC 1 cut(s) 2251
AfiI CCNNNNNNNGG 7 cut(s) 1094, 1118, 1367, 2476, 2539, 3535, 4222
AflIII ACRYGT 3 cut(s) 2916, 3379, 3754
AgeI ACCGGT 1 cut(s) 5585
AhlI ACTAGT 1 cut(s) 3584
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
AjuI GAANNNNNNNTTGG 2 cut(s) 5540, 5572
AleI CACNNNNGTG 2 cut(s) 524, 5614
AloI GAACNNNNNNTCC 4 cut(s) 231, 263, 5178, 5210
Alw21I GWGCWC 4 cut(s) 415, 762, 2821, 3519
Alw26I GTCTC 7 cut(s) 117, 1742, 1927, 1929, 2931, 3325, 3753
AlwNI CAGNNNCTG 1 cut(s) 3929
Ama87I CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
AoxI GGCC 9 cut(s) 662, 1286, 2310, 2424, 2555, 3460, 3522, 4725, 5017
ApoI RAATTY 6 cut(s) 1780, 3187, 3650, 3819, 4067, 4928
ArsI GACNNNNNNTTYG 4 cut(s) 231, 263, 3355, 3387
AseI ATTAAT 1 cut(s) 3554
AsiGI ACCGGT 1 cut(s) 5585
Asp700I GAANNNNTTC 3 cut(s) 863, 1332, 4141
AspLEI GCGC 5 cut(s) 268, 635, 3033, 4883, 4959
AspS9I GGNCC 5 cut(s) 17, 2890, 2956, 3247, 3926
AsuNHI GCTAGC 2 cut(s) 4207, 4211
AvaI CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
AvaII GGWCC 5 cut(s) 17, 2890, 2956, 3247, 3926
BaeGI GKGCMC 1 cut(s) 4500
BaeI ACNNNNGTAYC 2 cut(s) 4592, 4625
BalI TGGCCA 3 cut(s) 664, 2557, 5019
BanI GGYRCC 2 cut(s) 103, 4497
BanII GRGCYC 2 cut(s) 3519, 3625
BarI GAAGNNNNNNTAC 4 cut(s) 621, 653, 3157, 3189
BbsI GAAGAC 7 cut(s) 699, 1134, 1528, 1733, 3597, 4329, 5440
Bbv12I GWGCWC 4 cut(s) 415, 762, 2821, 3519
BccI CCATC 9 cut(s) 115, 1371, 1375, 2413, 2860, 3154, 3308, 4717, 4735
BceAI ACGGC 4 cut(s) 1581, 4461, 4619, 5651
BciT130I CCWGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
BclI TGATCA 2 cut(s) 1479, 5052
BcoDI GTCTC 7 cut(s) 117, 1742, 1927, 1929, 2931, 3325, 3753
BcuI ACTAGT 1 cut(s) 3584
BfmI CTRYAG 5 cut(s) 624, 846, 2208, 4942, 5026
BfuAI ACCTGC 1 cut(s) 3937
BglI GCCNNNNNGGC 1 cut(s) 40
BglII AGATCT 1 cut(s) 3345
BmcAI AGTACT 1 cut(s) 4561
Bme1390I CCNGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
Bme18I GGWCC 5 cut(s) 17, 2890, 2956, 3247, 3926
BmeT110I CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
BmgBI CACGTC 1 cut(s) 242
BmgT120I GGNCC 5 cut(s) 17, 2890, 2956, 3247, 3926
BmiI GGNNCC 6 cut(s) 105, 2331, 2403, 2958, 4499, 5732
BmrFI CCNGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
BmrI ACTGGG 3 cut(s) 3053, 4844, 4908
BmsI GCATC 9 cut(s) 316, 1291, 1527, 1563, 1896, 3202, 3458, 3605, 4785
BmtI GCTAGC 2 cut(s) 4211, 4215
BmuI ACTGGG 3 cut(s) 3053, 4844, 4908
BpiI GAAGAC 7 cut(s) 699, 1134, 1528, 1733, 3597, 4329, 5440
BplI GAGNNNNNCTC 4 cut(s) 1394, 1426, 2335, 2367
BpmI CTGGAG 4 cut(s) 1389, 2746, 3503, 5184
BpuEI CTTGAG 2 cut(s) 2833, 4775
Bsa29I ATCGAT 1 cut(s) 1375
BsaAI YACGTR 4 cut(s) 550, 3741, 3755, 5804
BsaBI GATNNNNATC 1 cut(s) 2541
BsaHI GRCGYC 1 cut(s) 2251
BsaI GGTCTC 2 cut(s) 1742, 1929
BsaJI CCNNGG 5 cut(s) 659, 821, 1525, 5179, 5547
BsaWI WCCGGW 4 cut(s) 3698, 3909, 5585, 5710
BsaXI ACNNNNNCTCC 4 cut(s) 1069, 1099, 5207, 5237
Bsc4I CCNNNNNNNGG 7 cut(s) 1094, 1118, 1367, 2476, 2539, 3535, 4222
Bse118I RCCGGY 2 cut(s) 1111, 5585
Bse1I ACTGG 8 cut(s) 253, 710, 1372, 1766, 3059, 4839, 4903, 5325
Bse3DI GCAATG 5 cut(s) 1482, 1844, 2620, 4369, 5604
Bse8I GATNNNNATC 1 cut(s) 2541
BseBI CCWGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
BseCI ATCGAT 1 cut(s) 1375
BseDI CCNNGG 5 cut(s) 659, 821, 1525, 5179, 5547
BseJI GATNNNNATC 1 cut(s) 2541
BseLI CCNNNNNNNGG 7 cut(s) 1094, 1118, 1367, 2476, 2539, 3535, 4222
BseMI GCAATG 5 cut(s) 1482, 1844, 2620, 4369, 5604
BseNI ACTGG 8 cut(s) 253, 710, 1372, 1766, 3059, 4839, 4903, 5325
BseRI GAGGAG 5 cut(s) 1215, 2489, 2894, 3006, 5046
BseSI GKGCMC 1 cut(s) 4500
BseYI CCCAGC 2 cut(s) 1073, 4492
BsgI GTGCAG 2 cut(s) 4668, 4727
Bsh1236I CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
BshFI GGCC 9 cut(s) 664, 1288, 2312, 2426, 2557, 3462, 3524, 4727, 5019
BshNI GGYRCC 2 cut(s) 103, 4497
BshTI ACCGGT 1 cut(s) 5585
BshVI ATCGAT 1 cut(s) 1375
BsiHKAI GWGCWC 4 cut(s) 415, 762, 2821, 3519
BsiHKCI CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
BsiSI CCGG 5 cut(s) 1112, 3699, 3910, 5586, 5711
BslFI GGGAC 4 cut(s) 981, 1785, 2942, 4852
BslI CCNNNNNNNGG 7 cut(s) 1094, 1118, 1367, 2476, 2539, 3535, 4222
BsmAI GTCTC 7 cut(s) 117, 1742, 1927, 1929, 2931, 3325, 3753
BsmBI CGTCTC 2 cut(s) 117, 3753
BsmFI GGGAC 4 cut(s) 981, 1785, 2942, 4852
BsmI GAATGC 4 cut(s) 1025, 1216, 2024, 5766
BsnI GGCC 9 cut(s) 664, 1288, 2312, 2426, 2557, 3462, 3524, 4727, 5019
Bso31I GGTCTC 2 cut(s) 1742, 1929
BsoBI CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
Bsp1286I GDGCHC 6 cut(s) 415, 762, 2821, 3519, 3625, 4500
Bsp19I CCATGG 1 cut(s) 659
BspANI GGCC 9 cut(s) 664, 1288, 2312, 2426, 2557, 3462, 3524, 4727, 5019
BspDI ATCGAT 1 cut(s) 1375
BspFNI CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
BspHI TCATGA 1 cut(s) 4375
BspLI GGNNCC 6 cut(s) 105, 2331, 2403, 2958, 4499, 5732
BspMI ACCTGC 1 cut(s) 3937
BspOI GCTAGC 2 cut(s) 4211, 4215
BspQI GCTCTTC 6 cut(s) 899, 1856, 2336, 2826, 5535, 5839
BspT107I GGYRCC 2 cut(s) 103, 4497
BspTNI GGTCTC 2 cut(s) 1742, 1929
BsrBI CCGCTC 1 cut(s) 5832
BsrDI GCAATG 5 cut(s) 1482, 1844, 2620, 4369, 5604
BsrFI RCCGGY 2 cut(s) 1111, 5585
BsrI ACTGG 8 cut(s) 253, 710, 1372, 1766, 3059, 4839, 4903, 5325
BssAI RCCGGY 2 cut(s) 1111, 5585
BssECI CCNNGG 5 cut(s) 659, 821, 1525, 5179, 5547
BssNI GRCGYC 1 cut(s) 2251
BssT1I CCWWGG 4 cut(s) 659, 821, 1525, 5547
Bst2UI CCWGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
BstACI GRCGYC 1 cut(s) 2251
BstAPI GCANNNNNTGC 2 cut(s) 2435, 4204
BstBAI YACGTR 4 cut(s) 550, 3741, 3755, 5804
BstDSI CCRYGG 1 cut(s) 659
BstEII GGTNACC 2 cut(s) 2160, 3701
BstFNI CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
BstHHI GCGC 5 cut(s) 268, 635, 3033, 4883, 4959
BstMAI GTCTC 7 cut(s) 117, 1742, 1927, 1929, 2931, 3325, 3753
BstNI CCWGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
BstNSI RCATGY 7 cut(s) 700, 1106, 2920, 2968, 3235, 4522, 4672
BstPI GGTNACC 2 cut(s) 2160, 3701
BstSCI CCNGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
BstSFI CTRYAG 5 cut(s) 624, 846, 2208, 4942, 5026
BstSLI GKGCMC 1 cut(s) 4500
BstSNI TACGTA 1 cut(s) 3741
BstUI CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
BstV2I GAAGAC 7 cut(s) 699, 1134, 1528, 1733, 3597, 4329, 5440
BstX2I RGATCY 6 cut(s) 1499, 2262, 2542, 2580, 3345, 3695
BstXI CCANNNNNNTGG 3 cut(s) 3360, 3865, 5589
BstYI RGATCY 6 cut(s) 1499, 2262, 2542, 2580, 3345, 3695
Bsu15I ATCGAT 1 cut(s) 1375
BsuRI GGCC 9 cut(s) 664, 1288, 2312, 2426, 2557, 3462, 3524, 4727, 5019
BsuTUI ATCGAT 1 cut(s) 1375
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 242
BtsIMutI CAGTG 7 cut(s) 100, 3066, 3467, 3632, 3688, 4896, 5055
BveI ACCTGC 1 cut(s) 3937
CaiI CAGNNNCTG 1 cut(s) 3929
CciI TCATGA 1 cut(s) 4375
CfoI GCGC 5 cut(s) 268, 635, 3033, 4883, 4959
Cfr10I RCCGGY 2 cut(s) 1111, 5585
Cfr13I GGNCC 5 cut(s) 17, 2890, 2956, 3247, 3926
ClaI ATCGAT 1 cut(s) 1375
CseI GACGC 2 cut(s) 3602, 5671
CsiI ACCWGGT 1 cut(s) 3897
CspAI ACCGGT 1 cut(s) 5585
CspCI CAANNNNNGTGG 6 cut(s) 1671, 1706, 2205, 2240, 5345, 5380
DraI TTTAAA 3 cut(s) 2235, 2698, 5344
DrdI GACNNNNNNGTC 1 cut(s) 2954
DseDI GACNNNNNNGTC 1 cut(s) 2954
EaeI YGGCCR 3 cut(s) 662, 2555, 5017
EciI GGCGGA 3 cut(s) 15, 21, 58
Ecl136II GAGCTC 1 cut(s) 3517
Eco105I TACGTA 1 cut(s) 3741
Eco130I CCWWGG 4 cut(s) 659, 821, 1525, 5547
Eco147I AGGCCT 2 cut(s) 2312, 3524
Eco24I GRGCYC 2 cut(s) 3519, 3625
Eco31I GGTCTC 2 cut(s) 1742, 1929
Eco47I GGWCC 5 cut(s) 17, 2890, 2956, 3247, 3926
Eco53kI GAGCTC 1 cut(s) 3517
Eco57I CTGAAG 2 cut(s) 4540, 5452
Eco88I CYCGRG 4 cut(s) 3575, 5254, 5276, 5670
Eco91I GGTNACC 2 cut(s) 2160, 3701
EcoICRI GAGCTC 1 cut(s) 3517
EcoO109I RGGNCCY 3 cut(s) 17, 2956, 3926
EcoO65I GGTNACC 2 cut(s) 2160, 3701
EcoRII CCWGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
EcoT14I CCWWGG 4 cut(s) 659, 821, 1525, 5547
EcoT22I ATGCAT 1 cut(s) 1959
EcoT38I GRGCYC 2 cut(s) 3519, 3625
ErhI CCWWGG 4 cut(s) 659, 821, 1525, 5547
Esp3I CGTCTC 2 cut(s) 117, 3753
FaqI GGGAC 4 cut(s) 981, 1785, 2942, 4852
FauNDI CATATG 2 cut(s) 1044, 1117
FbaI TGATCA 2 cut(s) 1479, 5052
FblI GTMKAC 2 cut(s) 2097, 2652
FriOI GRGCYC 2 cut(s) 3519, 3625
GlaI GCGC 5 cut(s) 267, 634, 3032, 4882, 4958
GsaI CCCAGC 2 cut(s) 1077, 4496
GsuI CTGGAG 4 cut(s) 1389, 2746, 3503, 5184
HaeIII GGCC 9 cut(s) 664, 1288, 2312, 2426, 2557, 3462, 3524, 4727, 5019
HapII CCGG 5 cut(s) 1112, 3699, 3910, 5586, 5711
HgaI GACGC 2 cut(s) 3602, 5671
HhaI GCGC 5 cut(s) 268, 635, 3033, 4883, 4959
Hin1I GRCGYC 1 cut(s) 2251
Hin6I GCGC 5 cut(s) 266, 633, 3031, 4881, 4957
HinP1I GCGC 5 cut(s) 266, 633, 3031, 4881, 4957
HincII GTYRAC 4 cut(s) 2653, 2976, 3582, 4569
HindII GTYRAC 4 cut(s) 2653, 2976, 3582, 4569
HindIII AAGCTT 7 cut(s) 378, 1134, 1580, 2018, 4038, 4296, 4653
HpaI GTTAAC 2 cut(s) 3582, 4569
HpaII CCGG 5 cut(s) 1112, 3699, 3910, 5586, 5711
Hpy99I CGWCG 5 cut(s) 928, 1875, 2253, 3008, 3616
Hsp92I GRCGYC 1 cut(s) 2251
HspAI GCGC 5 cut(s) 266, 633, 3031, 4881, 4957
Ksp22I TGATCA 2 cut(s) 1479, 5052
KspAI GTTAAC 2 cut(s) 3582, 4569
LguI GCTCTTC 6 cut(s) 899, 1856, 2336, 2826, 5535, 5839
LweI GCATC 9 cut(s) 316, 1291, 1527, 1563, 1896, 3202, 3458, 3605, 4785
MabI ACCWGGT 1 cut(s) 3897
MbiI CCGCTC 1 cut(s) 5832
MfeI CAATTG 1 cut(s) 4542
MflI RGATCY 6 cut(s) 1499, 2262, 2542, 2580, 3345, 3695
MhlI GDGCHC 6 cut(s) 415, 762, 2821, 3519, 3625, 4500
MlsI TGGCCA 3 cut(s) 664, 2557, 5019
MluI ACGCGT 1 cut(s) 3379
MluNI TGGCCA 3 cut(s) 664, 2557, 5019
MmeI TCCRAC 8 cut(s) 69, 881, 1894, 2704, 3174, 3405, 4689, 4855
Mox20I TGGCCA 3 cut(s) 664, 2557, 5019
Mph1103I ATGCAT 1 cut(s) 1959
MroXI GAANNNNTTC 3 cut(s) 863, 1332, 4141
MscI TGGCCA 3 cut(s) 664, 2557, 5019
MslI CAYNNNNRTG 7 cut(s) 524, 1962, 3203, 4248, 4854, 5078, 5614
Msp20I TGGCCA 3 cut(s) 664, 2557, 5019
MspA1I CMGCKG 8 cut(s) 395, 668, 949, 1325, 1679, 1722, 2103, 2553
MspI CCGG 5 cut(s) 1112, 3699, 3910, 5586, 5711
MspR9I CCNGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
MunI CAATTG 1 cut(s) 4542
Mva1269I GAATGC 4 cut(s) 1025, 1216, 2024, 5766
MvaI CCWGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
MvnI CGCG 5 cut(s) 333, 1856, 3381, 3987, 4957
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 2 cut(s) 1044, 1117
NheI GCTAGC 2 cut(s) 4207, 4211
NlaIV GGNNCC 6 cut(s) 105, 2331, 2403, 2958, 4499, 5732
NmeAIII GCCGAG 1 cut(s) 1956
NmuCI GTSAC 9 cut(s) 671, 1153, 1971, 2160, 2929, 3701, 4366, 4478, 5080
NsiI ATGCAT 1 cut(s) 1959
NspI RCATGY 7 cut(s) 700, 1106, 2920, 2968, 3235, 4522, 4672
OliI CACNNNNGTG 2 cut(s) 524, 5614
PaeI GCATGC 2 cut(s) 2968, 4522
PaeR7I CTCGAG 3 cut(s) 3575, 5276, 5670
PagI TCATGA 1 cut(s) 4375
PceI AGGCCT 2 cut(s) 2312, 3524
PciI ACATGT 1 cut(s) 2916
PciSI GCTCTTC 6 cut(s) 899, 1856, 2336, 2826, 5535, 5839
PctI GAATGC 4 cut(s) 1025, 1216, 2024, 5766
PdmI GAANNNNTTC 3 cut(s) 863, 1332, 4141
PflFI GACNNNGTC 1 cut(s) 3899
PflMI CCANNNNNTGG 1 cut(s) 1367
PfoI TCCNGGA 1 cut(s) 2762
PinAI ACCGGT 1 cut(s) 5585
Ppu21I YACGTR 4 cut(s) 550, 3741, 3755, 5804
PpuMI RGGWCCY 3 cut(s) 17, 2956, 3926
PscI ACATGT 1 cut(s) 2916
PshBI ATTAAT 1 cut(s) 3554
Psp124BI GAGCTC 1 cut(s) 3519
Psp5II RGGWCCY 3 cut(s) 17, 2956, 3926
Psp6I CCWGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
PspEI GGTNACC 2 cut(s) 2160, 3701
PspFI CCCAGC 2 cut(s) 1073, 4492
PspGI CCWGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
PspN4I GGNNCC 6 cut(s) 105, 2331, 2403, 2958, 4499, 5732
PspPI GGNCC 5 cut(s) 17, 2890, 2956, 3247, 3926
PspPPI RGGWCCY 3 cut(s) 17, 2956, 3926
PspXI VCTCGAGB 1 cut(s) 5670
PsrI GAACNNNNNNTAC 2 cut(s) 1976, 2008
PstNI CAGNNNCTG 1 cut(s) 3929
PsuI RGATCY 6 cut(s) 1499, 2262, 2542, 2580, 3345, 3695
PsyI GACNNNGTC 1 cut(s) 3899
PvuII CAGCTG 3 cut(s) 668, 1679, 1722
RseI CAYNNNNRTG 7 cut(s) 524, 1962, 3203, 4248, 4854, 5078, 5614
SacI GAGCTC 1 cut(s) 3519
SalI GTCGAC 1 cut(s) 2651
SapI GCTCTTC 6 cut(s) 899, 1856, 2336, 2826, 5535, 5839
Sau96I GGNCC 5 cut(s) 17, 2890, 2956, 3247, 3926
ScaI AGTACT 1 cut(s) 4561
ScrFI CCNGG 7 cut(s) 2764, 3082, 3521, 3899, 3924, 4019, 5723
SduI GDGCHC 6 cut(s) 415, 762, 2821, 3519, 3625, 4500
SexAI ACCWGGT 1 cut(s) 3897
SfaNI GCATC 9 cut(s) 316, 1291, 1527, 1563, 1896, 3202, 3458, 3605, 4785
SfcI CTRYAG 5 cut(s) 624, 846, 2208, 4942, 5026
Sfr274I CTCGAG 3 cut(s) 3575, 5276, 5670
SinI GGWCC 5 cut(s) 17, 2890, 2956, 3247, 3926
SlaI CTCGAG 3 cut(s) 3575, 5276, 5670
SmiMI CAYNNNNRTG 7 cut(s) 524, 1962, 3203, 4248, 4854, 5078, 5614
SmlI CTYRAG 5 cut(s) 2812, 3575, 4754, 5276, 5670
SmoI CTYRAG 5 cut(s) 2812, 3575, 4754, 5276, 5670
SnaBI TACGTA 1 cut(s) 3741
SpeI ACTAGT 1 cut(s) 3584
SphI GCATGC 2 cut(s) 2968, 4522
SseBI AGGCCT 2 cut(s) 2312, 3524
SspI AATATT 2 cut(s) 1253, 3038
SstI GAGCTC 1 cut(s) 3519
StuI AGGCCT 2 cut(s) 2312, 3524
StyD4I CCNGG 7 cut(s) 2762, 3080, 3519, 3897, 3922, 4017, 5721
StyI CCWWGG 4 cut(s) 659, 821, 1525, 5547
TaqII GACCGA 1 cut(s) 3235
TatI WGTACW 5 cut(s) 1983, 2002, 3063, 4559, 4789
TauI GCSGC 4 cut(s) 395, 1518, 2429, 4881
TscAI CASTG 7 cut(s) 100, 3066, 3474, 3632, 3688, 4903, 5062
TseFI GTSAC 9 cut(s) 671, 1153, 1971, 2160, 2929, 3701, 4366, 4478, 5080
Tsp45I GTSAC 9 cut(s) 671, 1153, 1971, 2160, 2929, 3701, 4366, 4478, 5080
TspGWI ACGGA 3 cut(s) 4467, 4804, 5331
TspRI CASTG 7 cut(s) 100, 3066, 3474, 3632, 3688, 4903, 5062
Tth111I GACNNNGTC 1 cut(s) 3899
Van91I CCANNNNNTGG 1 cut(s) 1367
VpaK11BI GGWCC 5 cut(s) 17, 2890, 2956, 3247, 3926
VspI ATTAAT 1 cut(s) 3554
XapI RAATTY 6 cut(s) 1780, 3187, 3650, 3819, 4067, 4928
XbaI TCTAGA 1 cut(s) 2288
XceI RCATGY 7 cut(s) 700, 1106, 2920, 2968, 3235, 4522, 4672
XcmI CCANNNNNNNNNTGG 3 cut(s) 2134, 3292, 5208
XhoI CTCGAG 3 cut(s) 3575, 5276, 5670
XmiI GTMKAC 2 cut(s) 2097, 2652
XmnI GAANNNNTTC 3 cut(s) 863, 1332, 4141
ZraI GACGTC 1 cut(s) 2252
ZrmI AGTACT 1 cut(s) 4561
Zsp2I ATGCAT 1 cut(s) 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.