Rh1DG002500

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002059
Physical Location & Seq
Forward (+)
419104 .. 447970
28867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG002500.1

Sequence Viewer

Length: 5631 bp
ATGGCACATAGAAGGGACTTCGACCAGCAGCAACCGCAGAGGCGGATTCTGCGGACTCAGACTGCCGGAAGTTTTGGAGAGCCTATGTTGGATAGTGAGGTTGTGCCTTCTTCTCTGGTTGATATTGCTCCTATTCTCCGTGTTGCAAATGAAGTCGAATCTAGGAATGCAAGAGTTGCCTATCTCTGTCGCTTCTATGCATTTGAGAAAGCTCACAGACTAGATCCCACATCTAGTGGCCGTGGTGTTCGCCAGCTTAAAACCGCACTTCTTCAACGTCTAGAGAGGGAAAATGAAACAACATTGGCAGGACGAGCAAAGAGTGATGCTCGTGAAATGCAGAGTTTTTATCGAGATTACTACAAAAAATACATTCAAGCTTTGAACAGTACTGCTGATAAACCTGATCGTGCACAACTGACAAAGGCATACAAAACTGCTGCTGTTTTGTTTGAGGTTTTGAAGGCTGTCAATCAGACAGAGGCTGTTGAAGTCGCTGAGGAGATTTTGGAAGCCCACACTAAGGTTGAAGAAAAACAACAGATATATCCACCTTACAATATTCTTCCTCTTGATCCTGATAGTCGAAATCAGGCAATCATGAGATGTCCGGAGATTCGTGCTACTGTTTCTGCACTCCGAAACACCAGAGGTTTACCCTGGTCGAAGGACTACGAAAAGAAAGAAGATGAAGACATTCTTGATTGGCTTCAAGCTATGTTTGGTTTTCAGAAGGATAGTGTCGCCAATCAAAGGGAGCACTTGATTCTATTGATCGCTAATGTTCACATACGGCAATTACCAAAATCTGATCAGCAGCCCAAGATGGCATTTGAATTATATGGAATGCTAGCCGGGAGTGTTAGTCCAATGACGGGTGAGCACATAAAGCCAGCCTATGGTGGTGAAGAGGAGGCCTTTTTGGGCAAAGTAGTAACTCCAATATATAATACTATAGCACAGGAGGCTGAAAGGAGCAAAGGAGGAAAATCAAAGCATTCTCAATGGAGAAATTATGATGACCTGAATGAATACTTTTGGTCAGTTGATTGTTTTAAGCTAGGCTGGCCTATGCGTGCTGATGCTGATTTCTTTTACCAGCCTAGTAAAGAGCTTGAAGTTGACAAAGAGAAGAAAAAGAAACCATACACCGGGGATCGATGGATTGGCAAAGTCAATTTTGTCGAGATACGCTCATTTTGGCACCTTTTCAGAAGTTTTGATAGAATGTGGAGCTTTTATATATTGAGTTTACAGGCAATGATCATAGTTGCTTGGAATGGGTCAGGGGAATTAAGTACAGTGTTTGACGGTGATGTCTTTAAAAAAGTGCTGAGTATCTTCATAACTGCTGCAATGTTGAAGCTTGCGCAAGCTGTTCTCGATCTAATTCTGAGCTGGAAAGCTAGGCGAAGCATGTCAGCCTTTGTCAGGCTAAGATATGTCCTGAAAGCTGTTTCAGCTGCAGCCTGGGTCATTGTTTTACCTGTGACTTATGCATTTAGCTGGAAAAATCCCTCTGGGTTTGCACAGACCATCAGGAGTTGGTTTGGCAACGGTCCAAGTTCATCTTCTTTGTTCATTTTGGCTATTGTCATTTACTTATCTCCAAACATGCTCTCAGCATTGTTGTTTATGTTCCCATTTATTCGCCGGCATCTTGAAAGATCAAACTATAGGATTATGATGCTGATGATGTGGTGGTCTCAGCCTCGGCTTTATGTTGGAAGAGGAATGCATGAAAGCGCGTTTTCCCTTTTCAAGTATACCCTGTTTTGGGTTCTCCTGCTTGTTTCGAAATTAGCCTTTAGTTACTTTGTCGAGATTAGGCCTCTTGTAAAGCCAACTAAAGATATCATGAGAGTGCACATTTCGACCTATCAATGGCATGAGTTCTTCCCTCGAGCGAAGAGTAACATTGGTGTAGTGATTGCACTCTGGGCTCCAATTGTTCTTGTGTACTTTATGGATACTCAAATTTGGTATGCCATCTTCTCCACAATATATGGAGGTATATATGGTGCATTTCGCCGGCTTGGAGAGATTCGGACTCTGGAGTTGTTGAGAAGTCGATTTGAATCACTGCCCCGTGCTTTTAATGCTTCCTTGATTCCTGAGGAGAAAAATGAGAACACTAAGAAGAAGGGACTAAAAGCCACTTTCTCTCGTAAATTTGATAAGATCCCATCTAATATAGAGAATGAGGCAGCAAAATTTGCACAGATGTGGAACGAAATAATCACTAGTTTCAGAGAAGAGGATCTGATAAGTGATAGGGAAATGAACTTATTGCTTGTCCCATATTGGGCTGACCCAGATTTGACCCTCATCCAGTGGCCTCCATTTCTACTTGCTAGCAAGATCCCTATAGCACTAGATATGGCTAAAGACAGCAAAGGTAAAGACAGTGAGCTGAAAAAGAGAATGAATACAGACAAGGACAGTTATATGCGCTGTGCAGTTAGAGAGTGCTATCTCTCATTTAAAAGTATTATCAATGTTTTAGTCCAAGGAGAACGCGAAAAGATGGTTATAGATGAAATTTTTTCCATAGTTGATGATCATATAGAAAAAGGAACACTGACGAAGGAAGTTAAATTGAGTGCTCTCCCAAGCCTCCATGAACAATTTATTAAGCTAATTGATTATCTGTTGAAAAACAAAAGGGAGGACAAGGACCAGGTTGTAATTGTTTTGCTTAACATGTTAGAAGTTGTGACAAGGGACCTAATAGAAGATGAAATTACCACCTTGGTAGATTCAAACCATGGTGGATCATATGGAAACGATGGGGGAATGAGACCTCTTAATCAACAAGATACATACTTCAGTGAACTGAATTTCCCAGTGACGCAACAATCAGAGGCTTGGAAAGAAAAGATCCGAAGGCTTCATCTATTGCTTACGGAGAAGGAGTCTGCTATGGATGTTCCATCTAACTTGGAAGCAAGAAGGCGTATTACTTTCTTTTCCAACTCATTGTTTATGGATATGCCTCCTGCACCCAAAGTTCAAAATATGCTTTCCTTCTCGGTTTTGACACCCTACTATTCAGAAGACGTTCTTTTCTCCATAGATCACCTGGAAAGGGAGAACGATGATGGTGTCTCTATTCTTTTCTACTTGCAAAAGATTTTTCCAGATGAATGGACCAACTTTCTTGAACGGGTCAAATGTGGTACTGAAGATGAACTCAGAGCAAGCGAAGACTTGGAAGAGAAACTCCGCCTATGGGCATCCTATCGAGGTCAAACATTGACCAAAACTGTACGAGGTATGATGTATTACCGGAAAGCGCTAGAACTTCAGGCCTTCCTGGATATGGCAAAAGATGAAGATCTAATGGAAGGGTACAAGGCTGCTGAATCAACGATCGAGGAACATTCAAAGGCTGAAAGGCCGCTATTGGCACAATGTCAAGCAGTAGTTGACATGAAATTCTCATATGTTGTTTCGTGCCAACAGTATGGAATTGATAAGCGATCTGGTGATGCTCGTGCCAAAGACATTTTGAAACTAATGGCAACTTACCCATCTCTTCGGGTAGCTTACATTGACGAGGTTGAAAAAACTAGTGAAGACAAATCCAAAAAAATGGTTAGGAAGGTCTACTATTCGGCTCTTGTAAAGGCGGCTCCTCCAACAAAGTCAATTGATTCTTCTGATCCAGTTCAGAGATTGGACCAGGATATTTATCGGATAAAGCTTCCTGGACCCGCAATACTAGGAGAAGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTGCAAACAATAGATATGAACCAGGATAACTATTTGGAAGAAGCATTCAAAATGAGGAACTTGCTGGAAGAATTTCTCAAAAACCATGATGGCAGACCCCCAACAATACTTGGGCTTAGGGAGCATATATTTACTGGCAGTGTATCATCTCTTGCATGGTTTATGTCAAATCAGGAGACTAGTTTTGTAACTATTGGGCAGAGATTGTTGGCTAGCCCTCTGAGAGTTAGATTCCATTATGGCCATCCAGATGTATTCGACAGACTGTTTCATCTGACTAGAGGGGGTATAAGCAAAGCTTCTAAAGTGATCAACTTAAGCGAGGACATATTTGCAGGTTTCAATTCTACCTTACGTGATGGCAAAGTCACTCATCATGAATATATACAAGTTGGTAAAGGACGGGATGTCGGCCTCAACCAGATCTCCATTTTTGAGGCAAAAATAGCTAATGGGAACGGTGAGCAGACACTGAGTCGTGACATCTATAGGCTTGGACACCGATTTGATTTCTTCCGAATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTTAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTCTAATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTTGGTACAAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACTGTACAGATATGGTTCATGGTTGGAACATGGCTTTTCGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATTGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTATTCTGGGATGCGCGGTATCGTTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAGAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATCGTTGTTTTGTTGCTGATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTCTTGTTTCGACTTGTTAAGGGATGTATGTTTATTACACTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTTTGATTGCGCAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCTGTCGGTTCAAACACTGGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCTTGGTTCCCTTTTGTTTCTGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGCCCCCGCGCTAGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1876

Amino Acids

216.08

Weight (kDa)

9.1

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 169 1.1e-14 Vta1 like
FKS1_dom1 PF14288 276 - 357 1.6e-19 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 780 - 969 8.1e-63 Callose synthase 1 domain
Glucan_synthase PF02364 977 - 1067 5.7e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1070 - 1769 3.3e-208 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1316
Acc16I TGCGCA 2 cut(s) 1371, 5388
Acc36I ACCTGC 1 cut(s) 4109
AccB1I GGYRCC 1 cut(s) 1203
AccB7I CCANNNNNTGG 1 cut(s) 899
AccBSI CCGCTC 1 cut(s) 4407
AccI GTMKAC 3 cut(s) 1766, 3609, 5091
AccII CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
AccIII TCCGGA 1 cut(s) 610
AclWI GGATC 9 cut(s) 218, 569, 1164, 2176, 2268, 2356, 2782, 2875, 3659
AcoI YGGCCR 2 cut(s) 238, 4024
AcsI RAATTY 9 cut(s) 1977, 2171, 2213, 2541, 2839, 3437, 3854, 4712, 5525
AcuI CTGAAG 3 cut(s) 2812, 3204, 3290
AcvI CACGTG 1 cut(s) 3773
AdeI CACNNNGTG 1 cut(s) 4565
AfeI AGCGCT 1 cut(s) 3297
AflII CTTAAG 1 cut(s) 4099
AflIII ACRYGT 2 cut(s) 2703, 3770
AhdI GACNNNNNGTC 2 cut(s) 4190, 4257
AhlI ACTAGT 3 cut(s) 2243, 3572, 3962
AjuI GAANNNNNNNTTGG 2 cut(s) 1555, 1587
AleI CACNNNNGTG 1 cut(s) 2224
AloI GAACNNNNNNTCC 4 cut(s) 2826, 2858, 4532, 4564
Alw21I GWGCWC 6 cut(s) 415, 762, 885, 1869, 2608, 5310
Alw26I GTCTC 4 cut(s) 1710, 2794, 3112, 3953
Alw44I GTGCAC 2 cut(s) 411, 1865
AlwI GGATC 9 cut(s) 218, 569, 1164, 2176, 2268, 2356, 2782, 2875, 3659
AlwNI CAGNNNCTG 2 cut(s) 485, 4255
Ama87I CYCGRG 1 cut(s) 1902
Aor13HI TCCGGA 1 cut(s) 610
Aor51HI AGCGCT 1 cut(s) 3297
ApaI GGGCCC 1 cut(s) 5609
ApaLI GTGCAC 2 cut(s) 411, 1865
ApeKI GCWGC 8 cut(s) 28, 440, 817, 1352, 1463, 1466, 2207, 3359
ApoI RAATTY 9 cut(s) 1977, 2171, 2213, 2541, 2839, 3437, 3854, 4712, 5525
AseI ATTAAT 1 cut(s) 4577
Asp700I GAANNNNTTC 3 cut(s) 696, 3060, 3855
AspA2I CCTAGG 1 cut(s) 5597
AspLEI GCGC 8 cut(s) 1372, 1748, 2454, 3298, 5049, 5082, 5389, 5615
AsuC2I CCSGG 3 cut(s) 856, 1153, 5489
AsuHPI GGTGA 9 cut(s) 890, 917, 1325, 3071, 3500, 4256, 4312, 5105, 5179
AsuII TTCGAA 2 cut(s) 1796, 4444
AsuNHI GCTAGC 4 cut(s) 850, 2354, 3995, 5615
AvaI CYCGRG 1 cut(s) 1902
AvaII GGWCC 6 cut(s) 1559, 2677, 2725, 3150, 3682, 3713
AvrII CCTAGG 1 cut(s) 5597
AxyI CCTNAGG 1 cut(s) 2115
BaeGI GKGCMC 3 cut(s) 415, 1869, 5609
BaeI ACNNNNGTAYC 2 cut(s) 5122, 5155
BalI TGGCCA 1 cut(s) 4026
BanI GGYRCC 1 cut(s) 1203
BanII GRGCYC 2 cut(s) 1945, 5609
BarI GAAGNNNNNNTAC 4 cut(s) 1976, 2008, 2944, 2976
BauI CACGAG 4 cut(s) 330, 3495, 5021, 5448
BbrPI CACGTG 1 cut(s) 3773
BbsI GAAGAC 4 cut(s) 699, 3063, 3213, 3585
Bbv12I GWGCWC 6 cut(s) 415, 762, 885, 1869, 2608, 5310
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 8 cut(s) 40, 427, 829, 1339, 1450, 1478, 2219, 3346
BceAI ACGGC 2 cut(s) 225, 809
BciVI GTATCC 2 cut(s) 1963, 5150
BclI TGATCA 5 cut(s) 811, 1263, 2560, 4092, 5169
BcnI CCSGG 3 cut(s) 856, 1153, 5489
BcoDI GTCTC 4 cut(s) 1710, 2794, 3112, 3953
BcuI ACTAGT 3 cut(s) 2243, 3572, 3962
BfmI CTRYAG 7 cut(s) 954, 1464, 1675, 2367, 4270, 4485, 4726
BfoI RGCGCY 1 cut(s) 3299
BfrI CTTAAG 1 cut(s) 4099
BfuAI ACCTGC 1 cut(s) 4109
BfuI GTATCC 2 cut(s) 1963, 5150
BglII AGATCT 2 cut(s) 3337, 4206
BlnI CCTAGG 1 cut(s) 5597
BmcAI AGTACT 2 cut(s) 391, 4429
Bme18I GGWCC 6 cut(s) 1559, 2677, 2725, 3150, 3682, 3713
BmeRI GACNNNNNGTC 2 cut(s) 4190, 4257
BmeT110I CYCGRG 1 cut(s) 1902
BmrI ACTGGG 1 cut(s) 2840
BmsI GCATC 8 cut(s) 316, 1072, 1666, 1677, 3245, 3481, 5034, 5035
BmtI GCTAGC 4 cut(s) 854, 2358, 3999, 5619
BmuI ACTGGG 1 cut(s) 2840
BpiI GAAGAC 4 cut(s) 699, 3063, 3213, 3585
BplI GAGNNNNNCTC 4 cut(s) 313, 345, 1178, 1210
BpmI CTGGAG 1 cut(s) 2075
Bpu10I CCTNAGC 2 cut(s) 498, 3899
Bpu14I TTCGAA 2 cut(s) 1796, 4444
BpuMI CCSGG 3 cut(s) 856, 1153, 5489
Bsa29I ATCGAT 1 cut(s) 1159
BsaAI YACGTR 3 cut(s) 3773, 4139, 5582
BsaBI GATNNNNATC 3 cut(s) 2077, 3336, 3693
BsaI GGTCTC 2 cut(s) 1710, 2794
BsaWI WCCGGW 2 cut(s) 610, 3288
BsaXI ACNNNNNCTCC 6 cut(s) 850, 880, 2900, 2930, 4193, 4223
Bse118I RCCGGY 2 cut(s) 1653, 2031
Bse1I ACTGG 9 cut(s) 2332, 2846, 3668, 3922, 4687, 4937, 5095, 5368, 5448
Bse21I CCTNAGG 1 cut(s) 2115
Bse3DI GCAATG 4 cut(s) 1266, 1362, 4511, 5075
Bse8I GATNNNNATC 3 cut(s) 2077, 3336, 3693
BseAI TCCGGA 1 cut(s) 610
BseCI ATCGAT 1 cut(s) 1159
BseGI GGATG 9 cut(s) 2328, 2932, 3236, 4027, 4195, 5049, 5267, 5335, 5372
BseJI GATNNNNATC 3 cut(s) 2077, 3336, 3693
BseMI GCAATG 4 cut(s) 1266, 1362, 4511, 5075
BseNI ACTGG 9 cut(s) 2332, 2846, 3668, 3922, 4687, 4937, 5095, 5368, 5448
BseRI GAGGAG 4 cut(s) 515, 926, 2132, 3627
BseSI GKGCMC 3 cut(s) 415, 1869, 5609
BseXI GCAGC 8 cut(s) 40, 427, 829, 1339, 1450, 1478, 2219, 3346
BsgI GTGCAG 6 cut(s) 618, 2478, 2985, 4542, 4686, 5247
Bsh1236I CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
Bsh1285I CGRYCG 2 cut(s) 3375, 4387
BshNI GGYRCC 1 cut(s) 1203
BshVI ATCGAT 1 cut(s) 1159
BsiEI CGRYCG 2 cut(s) 3375, 4387
BsiHKAI GWGCWC 6 cut(s) 415, 762, 885, 1869, 2608, 5310
BsiHKCI CYCGRG 1 cut(s) 1902
BsiSI CCGG 8 cut(s) 66, 611, 855, 1152, 1654, 2032, 3289, 5489
BslFI GGGAC 4 cut(s) 29, 2160, 2282, 2738
BsmAI GTCTC 4 cut(s) 1710, 2794, 3112, 3953
BsmFI GGGAC 4 cut(s) 29, 2160, 2282, 2738
Bso31I GGTCTC 2 cut(s) 1710, 2794
BsoBI CYCGRG 1 cut(s) 1902
Bsp119I TTCGAA 2 cut(s) 1796, 4444
Bsp120I GGGCCC 1 cut(s) 5605
Bsp1286I GDGCHC 8 cut(s) 415, 762, 885, 1869, 1945, 2608, 5310, 5609
Bsp13I TCCGGA 1 cut(s) 610
Bsp1407I TGTACA 1 cut(s) 4843
Bsp19I CCATGG 1 cut(s) 2767
BspDI ATCGAT 1 cut(s) 1159
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
BspHI TCATGA 3 cut(s) 600, 1857, 4159
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 1 cut(s) 4109
BspOI GCTAGC 4 cut(s) 854, 2358, 3999, 5619
BspPI GGATC 9 cut(s) 218, 569, 1164, 2176, 2268, 2356, 2782, 2875, 3659
BspT104I TTCGAA 2 cut(s) 1796, 4444
BspT107I GGYRCC 1 cut(s) 1203
BspTI CTTAAG 1 cut(s) 4099
BspTNI GGTCTC 2 cut(s) 1710, 2794
BsrBI CCGCTC 1 cut(s) 4407
BsrDI GCAATG 4 cut(s) 1266, 1362, 4511, 5075
BsrFI RCCGGY 2 cut(s) 1653, 2031
BsrGI TGTACA 1 cut(s) 4843
BsrI ACTGG 9 cut(s) 2332, 2846, 3668, 3922, 4687, 4937, 5095, 5368, 5448
BssAI RCCGGY 2 cut(s) 1653, 2031
BssNAI GTATAC 1 cut(s) 1767
BssSI CACGAG 4 cut(s) 330, 3495, 5021, 5448
BssT1I CCWWGG 4 cut(s) 2509, 2751, 2767, 5597
Bst1107I GTATAC 1 cut(s) 1767
Bst2BI CACGAG 4 cut(s) 330, 3495, 5021, 5448
Bst6I CTCTTC 7 cut(s) 903, 1723, 1904, 2250, 3210, 3543, 4464
BstAFI CTTAAG 1 cut(s) 4099
BstAPI GCANNNNNTGC 2 cut(s) 176, 2216
BstAUI TGTACA 1 cut(s) 4843
BstBAI YACGTR 3 cut(s) 3773, 4139, 5582
BstBI TTCGAA 2 cut(s) 1796, 4444
BstDSI CCRYGG 2 cut(s) 241, 2767
BstENI CCTNNNNNAGG 1 cut(s) 1429
BstF5I GGATG 9 cut(s) 2328, 2932, 3236, 4027, 4195, 5049, 5267, 5335, 5372
BstFNI CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
BstH2I RGCGCY 1 cut(s) 3299
BstHHI GCGC 8 cut(s) 1372, 1748, 2454, 3298, 5049, 5082, 5389, 5615
BstMAI GTCTC 4 cut(s) 1710, 2794, 3112, 3953
BstMCI CGRYCG 2 cut(s) 3375, 4387
BstNSI RCATGY 3 cut(s) 1420, 1618, 2707
BstSFI CTRYAG 7 cut(s) 954, 1464, 1675, 2367, 4270, 4485, 4726
BstSLI GKGCMC 3 cut(s) 415, 1869, 5609
BstUI CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
BstV1I GCAGC 8 cut(s) 40, 427, 829, 1339, 1450, 1478, 2219, 3346
BstV2I GAAGAC 4 cut(s) 699, 3063, 3213, 3585
BstX2I RGATCY 7 cut(s) 223, 2181, 2260, 2361, 2880, 3337, 4206
BstXI CCANNNNNNTGG 5 cut(s) 3147, 3467, 3595, 4799, 5367
BstYI RGATCY 7 cut(s) 223, 2181, 2260, 2361, 2880, 3337, 4206
BstZ17I GTATAC 1 cut(s) 1767
Bsu15I ATCGAT 1 cut(s) 1159
Bsu36I CCTNAGG 1 cut(s) 2115
BsuI GTATCC 2 cut(s) 1963, 5150
BsuTUI ATCGAT 1 cut(s) 1159
BtgI CCRYGG 2 cut(s) 241, 2767
BtsCI GGATG 9 cut(s) 2328, 2932, 3236, 4027, 4195, 5049, 5267, 5335, 5372
BtsI GCAGTG 2 cut(s) 2081, 3928
BveI ACCTGC 1 cut(s) 4109
CaiI CAGNNNCTG 2 cut(s) 485, 4255
CciI TCATGA 3 cut(s) 600, 1857, 4159
CfoI GCGC 8 cut(s) 1372, 1748, 2454, 3298, 5049, 5082, 5389, 5615
Cfr10I RCCGGY 2 cut(s) 1653, 2031
ClaI ATCGAT 1 cut(s) 1159
CseI GACGC 1 cut(s) 2860
CsiI ACCWGGT 1 cut(s) 2679
DraI TTTAAA 2 cut(s) 1324, 2485
DraIII CACNNNGTG 1 cut(s) 4565
DrdI GACNNNNNNGTC 1 cut(s) 1316
DriI GACNNNNNGTC 2 cut(s) 4190, 4257
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 2 cut(s) 238, 4024
Eam1104I CTCTTC 7 cut(s) 903, 1723, 1904, 2250, 3210, 3543, 4464
Eam1105I GACNNNNNGTC 2 cut(s) 4190, 4257
EarI CTCTTC 7 cut(s) 903, 1723, 1904, 2250, 3210, 3543, 4464
EciI GGCGGA 2 cut(s) 58, 3215
Eco130I CCWWGG 4 cut(s) 2509, 2751, 2767, 5597
Eco147I AGGCCT 3 cut(s) 917, 1831, 3311
Eco24I GRGCYC 2 cut(s) 1945, 5609
Eco31I GGTCTC 2 cut(s) 1710, 2794
Eco32I GATATC 1 cut(s) 1855
Eco47I GGWCC 6 cut(s) 1559, 2677, 2725, 3150, 3682, 3713
Eco47III AGCGCT 1 cut(s) 3297
Eco57I CTGAAG 3 cut(s) 2812, 3204, 3290
Eco72I CACGTG 1 cut(s) 3773
Eco81I CCTNAGG 1 cut(s) 2115
Eco88I CYCGRG 1 cut(s) 1902
EcoNI CCTNNNNNAGG 1 cut(s) 1429
EcoO109I RGGNCCY 4 cut(s) 2725, 5594, 5605, 5606
EcoRI GAATTC 1 cut(s) 5525
EcoRV GATATC 1 cut(s) 1855
EcoT14I CCWWGG 4 cut(s) 2509, 2751, 2767, 5597
EcoT22I ATGCAT 3 cut(s) 202, 1501, 1740
EcoT38I GRGCYC 2 cut(s) 1945, 5609
ErhI CCWWGG 4 cut(s) 2509, 2751, 2767, 5597
FaqI GGGAC 4 cut(s) 29, 2160, 2282, 2738
FauI CCCGC 2 cut(s) 3724, 5618
FauNDI CATATG 3 cut(s) 2779, 3445, 5316
FbaI TGATCA 5 cut(s) 811, 1263, 2560, 4092, 5169
FblI GTMKAC 3 cut(s) 1766, 3609, 5091
FokI GGATG 9 cut(s) 2315, 2939, 3223, 4014, 4202, 5056, 5274, 5342, 5379
FriOI GRGCYC 2 cut(s) 1945, 5609
FspI TGCGCA 2 cut(s) 1371, 5388
GlaI GCGC 8 cut(s) 1371, 1747, 2453, 3297, 5048, 5081, 5388, 5614
GsuI CTGGAG 1 cut(s) 2075
HaeII RGCGCY 1 cut(s) 3299
HapII CCGG 8 cut(s) 66, 611, 855, 1152, 1654, 2032, 3289, 5489
HgaI GACGC 1 cut(s) 2860
HhaI GCGC 8 cut(s) 1372, 1748, 2454, 3298, 5049, 5082, 5389, 5615
Hin6I GCGC 8 cut(s) 1370, 1746, 2452, 3296, 5047, 5080, 5387, 5613
HinP1I GCGC 8 cut(s) 1370, 1746, 2452, 3296, 5047, 5080, 5387, 5613
HincII GTYRAC 3 cut(s) 1123, 3430, 4353
HindII GTYRAC 3 cut(s) 1123, 3430, 4353
HindIII AAGCTT 5 cut(s) 378, 1364, 3704, 4080, 5391
HpaI GTTAAC 1 cut(s) 4353
HpaII CCGG 8 cut(s) 66, 611, 855, 1152, 1654, 2032, 3289, 5489
HphI GGTGA 9 cut(s) 890, 917, 1325, 3071, 3500, 4256, 4312, 5105, 5179
Hpy99I CGWCG 1 cut(s) 5220
HpyCH4IV ACGT 5 cut(s) 277, 3060, 3772, 4138, 5581
HpySE526I ACGT 5 cut(s) 277, 3060, 3772, 4138, 5581
HspAI GCGC 8 cut(s) 1370, 1746, 2452, 3296, 5047, 5080, 5387, 5613
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 2 cut(s) 1653, 2031
KroNI GCCGGC 2 cut(s) 1655, 2033
Ksp22I TGATCA 5 cut(s) 811, 1263, 2560, 4092, 5169
KspAI GTTAAC 1 cut(s) 4353
LmnI GCTCC 8 cut(s) 133, 757, 975, 1233, 1948, 3640, 3904, 4885
Lsp1109I GCAGC 8 cut(s) 40, 427, 829, 1339, 1450, 1478, 2219, 3346
LweI GCATC 8 cut(s) 316, 1072, 1666, 1677, 3245, 3481, 5034, 5035
MabI ACCWGGT 1 cut(s) 2679
MaeII ACGT 5 cut(s) 277, 3060, 3772, 4138, 5581
MbiI CCGCTC 1 cut(s) 4407
MfeI CAATTG 3 cut(s) 1947, 3651, 4589
MflI RGATCY 7 cut(s) 223, 2181, 2260, 2361, 2880, 3337, 4206
MhlI GDGCHC 8 cut(s) 415, 762, 885, 1869, 1945, 2608, 5310, 5609
MlsI TGGCCA 1 cut(s) 4026
MluNI TGGCCA 1 cut(s) 4026
MlyI GAGTC 5 cut(s) 49, 2044, 2924, 4267, 4624
MmeI TCCRAC 5 cut(s) 69, 1705, 2997, 3665, 4843
Mox20I TGGCCA 1 cut(s) 4026
Mph1103I ATGCAT 3 cut(s) 202, 1501, 1740
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 2 cut(s) 1653, 2031
MroXI GAANNNNTTC 3 cut(s) 696, 3060, 3855
MscI TGGCCA 1 cut(s) 4026
MslI CAYNNNNRTG 5 cut(s) 1862, 2224, 4032, 4662, 5315
Msp20I TGGCCA 1 cut(s) 4026
MspA1I CMGCKG 2 cut(s) 1463, 5207
MspCI CTTAAG 1 cut(s) 4099
MspI CCGG 8 cut(s) 66, 611, 855, 1152, 1654, 2032, 3289, 5489
MunI CAATTG 3 cut(s) 1947, 3651, 4589
MvnI CGCG 5 cut(s) 1748, 2520, 5049, 5536, 5613
NaeI GCCGGC 2 cut(s) 1655, 2033
NciI CCSGG 3 cut(s) 856, 1153, 5489
NcoI CCATGG 1 cut(s) 2767
NdeI CATATG 3 cut(s) 2779, 3445, 5316
NgoMIV GCCGGC 2 cut(s) 1653, 2031
NheI GCTAGC 4 cut(s) 850, 2354, 3995, 5615
NmeAIII GCCGAG 1 cut(s) 1693
NmuCI GTSAC 5 cut(s) 1489, 2716, 2848, 4150, 4262
NsbI TGCGCA 2 cut(s) 1371, 5388
NsiI ATGCAT 3 cut(s) 202, 1501, 1740
NspI RCATGY 3 cut(s) 1420, 1618, 2707
NspV TTCGAA 2 cut(s) 1796, 4444
OliI CACNNNNGTG 1 cut(s) 2224
PaeR7I CTCGAG 1 cut(s) 1902
PagI TCATGA 3 cut(s) 600, 1857, 4159
PceI AGGCCT 3 cut(s) 917, 1831, 3311
PciI ACATGT 1 cut(s) 2703
PcsI WCGNNNNNNNCGW 1 cut(s) 4441
PdiI GCCGGC 2 cut(s) 1655, 2033
PdmI GAANNNNTTC 3 cut(s) 696, 3060, 3855
PflMI CCANNNNNTGG 1 cut(s) 899
PfoI TCCNGGA 2 cut(s) 3315, 3709
Ple19I CGATCG 1 cut(s) 3375
PleI GAGTC 5 cut(s) 49, 2044, 2923, 4266, 4624
PmaCI CACGTG 1 cut(s) 3773
PmlI CACGTG 1 cut(s) 3773
PpsI GAGTC 5 cut(s) 49, 2044, 2923, 4266, 4624
Ppu21I YACGTR 3 cut(s) 3773, 4139, 5582
PpuMI RGGWCCY 1 cut(s) 2725
PscI ACATGT 1 cut(s) 2703
PshBI ATTAAT 1 cut(s) 4577
Psp5II RGGWCCY 1 cut(s) 2725
PspCI CACGTG 1 cut(s) 3773
PspOMI GGGCCC 1 cut(s) 5605
PspPPI RGGWCCY 1 cut(s) 2725
PspXI VCTCGAGB 1 cut(s) 1902
PstI CTGCAG 1 cut(s) 1468
PstNI CAGNNNCTG 2 cut(s) 485, 4255
PsuI RGATCY 7 cut(s) 223, 2181, 2260, 2361, 2880, 3337, 4206
PvuI CGATCG 1 cut(s) 3375
PvuII CAGCTG 2 cut(s) 1463, 5207
RseI CAYNNNNRTG 5 cut(s) 1862, 2224, 4032, 4662, 5315
ScaI AGTACT 2 cut(s) 391, 4429
SchI GAGTC 5 cut(s) 49, 2044, 2924, 4267, 4624
SduI GDGCHC 8 cut(s) 415, 762, 885, 1869, 1945, 2608, 5310, 5609
SexAI ACCWGGT 1 cut(s) 2679
SfaNI GCATC 8 cut(s) 316, 1072, 1666, 1677, 3245, 3481, 5034, 5035
SfcI CTRYAG 7 cut(s) 954, 1464, 1675, 2367, 4270, 4485, 4726
Sfr274I CTCGAG 1 cut(s) 1902
SfuI TTCGAA 2 cut(s) 1796, 4444
SinI GGWCC 6 cut(s) 1559, 2677, 2725, 3150, 3682, 3713
SlaI CTCGAG 1 cut(s) 1902
SmiMI CAYNNNNRTG 5 cut(s) 1862, 2224, 4032, 4662, 5315
SmlI CTYRAG 2 cut(s) 1902, 4099
SmoI CTYRAG 2 cut(s) 1902, 4099
SpeI ACTAGT 3 cut(s) 2243, 3572, 3962
SseBI AGGCCT 3 cut(s) 917, 1831, 3311
SspI AATATT 1 cut(s) 562
StuI AGGCCT 3 cut(s) 917, 1831, 3311
StyI CCWWGG 4 cut(s) 2509, 2751, 2767, 5597
TaiI ACGT 5 cut(s) 280, 3063, 3775, 4141, 5584
TatI WGTACW 6 cut(s) 389, 1298, 1959, 4427, 4671, 4843
TauI GCSGC 2 cut(s) 3403, 3635
TseFI GTSAC 5 cut(s) 1489, 2716, 2848, 4150, 4262
TseI GCWGC 8 cut(s) 28, 440, 817, 1352, 1463, 1466, 2207, 3359
Tsp45I GTSAC 5 cut(s) 1489, 2716, 2848, 4150, 4262
TspGWI ACGGA 4 cut(s) 128, 2921, 4541, 4656
Van91I CCANNNNNTGG 1 cut(s) 899
Vha464I CTTAAG 1 cut(s) 4099
VneI GTGCAC 2 cut(s) 411, 1865
VpaK11BI GGWCC 6 cut(s) 1559, 2677, 2725, 3150, 3682, 3713
VspI ATTAAT 1 cut(s) 4577
XagI CCTNNNNNAGG 1 cut(s) 1429
XapI RAATTY 9 cut(s) 1977, 2171, 2213, 2541, 2839, 3437, 3854, 4712, 5525
XbaI TCTAGA 1 cut(s) 280
XceI RCATGY 3 cut(s) 1420, 1618, 2707
XcmI CCANNNNNNNNNTGG 1 cut(s) 3079
XhoI CTCGAG 1 cut(s) 1902
XmaJI CCTAGG 1 cut(s) 5597
XmiI GTMKAC 3 cut(s) 1766, 3609, 5091
XmnI GAANNNNTTC 3 cut(s) 696, 3060, 3855
ZrmI AGTACT 2 cut(s) 391, 4429
Zsp2I ATGCAT 3 cut(s) 202, 1501, 1740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.