AT1G08840

DNA replication factor Dna2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002244
Physical Location & Seq
Reverse (-)
2829105 .. 2838855
9751 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08840.2

Sequence Viewer

Length: 3891 bp
ATGCCACCAAGAAAGAAGCCCAAGTCTTCAGCTTTGAAATCAAACAAACAATCTTCAGCTAATCATTCGTCACAACCTTCCACCTTTGGCATCCAGCAGTTGTTCCTTCGACATATTCAGAACTCCCAATCAACTTCCAACTCTCATACCTCCACTGCTGACCCAGTTGATCAACAGAATGTGAATGGTCTTGCCTCTGACACTGCAGTTTTAACGCCTCAAAACCCTCTAGGCACATCGAATGAGAAACCTGATGAGTCCAAAGATATGGACCAGCAACTCACGGAGGCATCTCCTAAGATCTCCAAAAACCTGAAGCGCTTCTCTCCTGGAATGCTGATAAAGCAAAGTCAAGATGACTGTGGTGGTGAGATAACTTGGAAAATCTCTCCAGTAAATGAAAGACTTCGGGCAGCTGCTAAGAACATTCCTAAGATGATGGACTTAACAGAAAATTCACTAGGGAAGCAATGTCCTACATCAGGTATAACTTCCAAGGTTGAGCAATGGTTATCTTCACCCTCAAAAAAAGCTTCCAAAAGACCAGCTTTTGCAACAAATAGGGTCATGGAAAGGGTAAATCCTTCCCCAGATGCAGAGTTTGAAATTGTTAACACTTCAAGTAGTGGAAACAGTCCTTTCCAGACCCCACCGTCTCTTTCATGCCCTCACAACAAGCTTCCTTGTACTGTAACGTGCAGCGGAGCTTGTGGATCTATGGGGGCTGGACAACATAAAAAGGCCTTGCTTGAACTATTAGATCAAGTGGAGGATGTCATCGCAGTTGATGACAAAACAACTGATGATGTGGGGATTGTGATGCCTCAAGCTCGAGTTAAAGATGATATCATCTCTTCTGTTGTTGATTGCGCTGTAGATGAAGGGCCAGTTTCTCTACCAAAAATGCAGAATTCCATAAATCCAGATAGCTATTTCCTTGTATTGGAGGTATCGGAGAAACGTGGCTCGGGTAGCTCATCTAAGGGTCAATGTCCTTATAAGGTTCTTCGCTTGCTAGATGAGCACACAGGAGTGGAATGCGCTTTGTATCTCTGGGATGAATGGTTTTACAGCACTGTATCACCAGGAGATTCAATCAATGTTATTGGAGAGTTTGATGGGGATGGCAAGTGTGACGTGGACCGTCAAAACAATTTCTTAATTGTTCATCCTGATACCCTTGTTGCCGGAACTAGGGTTGCTGCAAGTTTTGGTTGCCCAAGGCGAACCGTGCTAGATGAGAGGCTCAGATCCAATGAACACGCTACAGTAGCCTTACTTGGAACCTTGCAACACCAAGTTTTTCAGGCTGGTCTCTCGCAAGAATCTCCATCTGTAGATGGTTTGCAAGAATATGCAAGCACAGTGATTGAGAAAAGTATTGAGAGCTTATATGCATGCGGAGTGCATGAAGGAGACGTGAGATCAACTTTATTTAAAGCTATCCCGAAAATGTTGAATTGGATTGAGCATTTCAGATATTCGAAGGACTCAGAAGTATCAAAAGTTGATTTTGGTTCTACCATTGGGAAGAAAGCTGTTAAGGTATCAGAGGTAATTGATATTGAGGAGATGTCATGGGCCCCTAAATATGGTCTCAAAGGGATGATCGATGCTTCGGTCAGAGTGATAGTTGAATCTGACATGAACACAGTGAATGAGAAGATAATGCCCCTTGAGTTTAAATCTGGAAAAGCTCCTAGTGGTCAGTCATCAATAGAGCATTCTGCACAGGTGATCCTGTACACGCTCCTTATGTCTGAGAGGTACCTGAAGCATATTGACAATGGCCTTCTGTACTATCTACAGTCAGATCAGACCCAGGGAATTTCTGTTCAACGATCAGATTTGGTGGGTCTGATTATTCGTCGCAATGAACTTGCAAATGACATTCTTGTGGCGTCAACAACCCAGCAACTGCCGCCGATGTTACGGGCAGATGGTGGAAACACAGAGAGTAGCGGACTTGGTGATGTCTTTGACACACATGTTTCTCATCTATCAACTTTACATTTCAATTTCCTCCGACACTGGGACAGGCTAATTGACTTGGAAGGTAGAGAGATGCAGCTTCTACGGAAAGATATTGCGCATCCACATGGTTCAAAGGGCAGCCACTCAGCTAGTTATCTTTCTTCAATGGTTCTTGACGTGACAAATGGGTTTCAGCATCATAACTCTCATAAAGAAACTAGATTCATCTATCGTTTTGTACGTCAAAAATCATCTGAATCTAGAGAAAGAGTAACCAGTGAAGATATGATTAGGACTGGAAACCTTGCAACTGATGACCTGGATTGCAAACTTAGAACTGGAGATCGTGTGATTCTTCGCACAGAAGTCAGCCACCTGACCGTTGCAAATGGGATTATAGCAGATATTAGTCGCACACATATATCAGTTTCTTTATCCAAGCGATTACGTCTTCCTTGGAGCGAACCTTCTTCTGAGGTATCAAATCTCTCTCATGAGTTATGGAGAATTTACAAGGATGAATTCATGACATCATTTTCGGTTATGAGGTTCAATCTCATGCAGCTTTTCGTACAAAATGGACATAACATCAGGAAAATGATTGTGGATCTCGAGCCTCCTAGATTTGACAATGGATCTATACTGAGCCAGGATCCTGCAATATCATATATTTGGTCAGAAAAGAGTCTGAATAATGATCAGCGCCAAGCTATACTTAAGATACTCACTGCAAAGGACTATGCTTTGATATTAGGAATGCCTGGAACAGGAAAAACCTCCACAATGGTTCATGCTGTGAAAGCTTTGTTGATCAGAGGCTCATCTATTCTGCTTGCATCGTACACAAACTCTGCTGTTGATAATCTACTTATCAAACTAAAAGCACAGGGAATTGAGTTTCTACGTATTGGTAGAGACGAGGCTGTACACGAAGAAGTTCGAGAAAGTTGCTTTTCAGCCATGAATATGTGCAGTGTCGAGGACATAAAGAAAAAGTTGGACCAGGTCAAAGTTGTGGCCTCTACATGTTTGGGAATCAATAGTCCTTTGCTTGTGAATAGGCGATTTGATGTATGCATTATTGATGAAGCTGGCCAGATTGCCCTCCCTGTTTCGATAGGGCCCTTGCTGTTTGCATCTACCTTTGTACTTGTTGGTGACCACTATCAACTACCGCCACTGGTGCAGAGTACAGAGGCTAGAGAGAATGGGATGGGAATAAGCTTGTTTCGCAGGTTATCAGAAGCCCATCCTCAGGCAATTTCAGTGTTACAAAACCAGTACCGAATGTGTCGAGGTATTATGGAACTATCAAATGCCTTGATATATGGAGACAGATTATGCTGCGGTTCTGCTGAAGTAGCTGATGCCACACTTGTGCTTTCTACCTCCAGTTCTACTTCGCCATGGCTTAAAAAGGTTCTGGAGCCAACAAGAACAGTTGTATTTGTTAATACAGATATGCTGCGTGCTTTTGAAGCTAGGGATCAGAATGCGATCAACAATCCAGTTGAAGCTTCCATAATAGCTGAGATTGTAGAGGAGCTAGTTAACAATGGAGTGGATAGTAAAGATATTGGAATCATTACTCCTTATAACTCACAAGCCAGCCTCATTCAACATGCCATTCCGACAACTCCTGTGGAGATACATACTATTGACAAATATCAGGGAAGAGATAAAGACTGCATATTGGTATCTTTTGTGAGATCGCGAGAGAAACCAAGGAGCTCTGCTTCTTCACTGCTCGGAGATTGGCATAGGATCAACGTTGCTTTGACACGAGCAAAGAAAAAGCTGATAATGGTGGGATCACAAAGAACACTCTCAAGAGTTCCACTTCTGATGCTTCTTCTAAACAAGGTTAAGGAGCAGTCAGGTATCTTGAATCTGTTGCCGGGAGATTTAAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1296

Amino Acids

143.25

Weight (kDa)

6.59

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 341 - 543 6.9e-59 DNA replication factor Dna2
AAA_11 PF13086 907 - 996 1.5e-15 AAA domain
AAA_30 PF13604 907 - 1070 8.6e-10 AAA domain
UvrD-helicase PF00580 907 - 1000 7.2e-06 UvrD/REP helicase N-terminal domain
AAA_19 PF13245 911 - 1071 3.9e-08 AAA domain
AAA_11 PF13086 1007 - 1074 2.1e-16 AAA domain
AAA_12 PF13087 1083 - 1283 5e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 999, 3570
AasI GACNNNNNNGTC 1 cut(s) 652
Acc16I TGCGCA 1 cut(s) 2091
Acc36I ACCTGC 1 cut(s) 3198
Acc65I GGTACC 1 cut(s) 1767
AccB1I GGYRCC 1 cut(s) 1767
AccII CGCG 1 cut(s) 3688
AciI CCGC 6 cut(s) 702, 1401, 1922, 1962, 3149, 3321
AclI AACGTT 1 cut(s) 3744
AcoI YGGCCR 1 cut(s) 3067
AcsI RAATTY 5 cut(s) 454, 910, 1827, 2481, 2495
AcuI CTGAAG 5 cut(s) 12, 39, 335, 1793, 3351
AcyI GRCGYC 1 cut(s) 1901
AfeI AGCGCT 1 cut(s) 320
AfiI CCNNNNNNNGG 7 cut(s) 482, 943, 1194, 1592, 2032, 2741, 3229
AflII CTTAAG 1 cut(s) 2690
AflIII ACRYGT 2 cut(s) 1987, 2999
AjiI CACGTC 3 cut(s) 1138, 1420, 2152
AjnI CCWGG 7 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976
AleI CACNNNNGTG 2 cut(s) 1031, 3350
AloI GAACNNNNNNTCC 2 cut(s) 1818, 1850
Alw21I GWGCWC 2 cut(s) 1026, 3707
Alw26I GTCTC 6 cut(s) 660, 1319, 1410, 1601, 2883, 3300
AlwNI CAGNNNCTG 1 cut(s) 1918
Ama87I CYCGRG 3 cut(s) 831, 967, 2585
Aor51HI AGCGCT 1 cut(s) 320
AoxI GGCC 7 cut(s) 741, 884, 1581, 1789, 2991, 3067, 3095
ApaI GGGCCC 2 cut(s) 1585, 3099
ApeKI GCWGC 9 cut(s) 413, 416, 699, 1202, 2068, 2112, 2536, 3318, 3439
ApoI RAATTY 5 cut(s) 454, 910, 1827, 2481, 2495
ArsI GACNNNNNNTTYG 2 cut(s) 2494, 2526
Asp700I GAANNNNTTC 3 cut(s) 320, 405, 2910
Asp718I GGTACC 1 cut(s) 1767
AspLEI GCGC 5 cut(s) 321, 872, 1043, 2092, 2679
AspS9I GGNCC 8 cut(s) 271, 884, 1141, 1581, 1582, 2974, 3095, 3096
AsuC2I CCSGG 1 cut(s) 3873
AsuHPI GGTGA 6 cut(s) 380, 510, 1074, 1747, 1982, 3143
AsuII TTCGAA 1 cut(s) 1484
AvaI CYCGRG 3 cut(s) 831, 967, 2585
AvaII GGWCC 3 cut(s) 271, 1141, 2974
AxyI CCTNAGG 1 cut(s) 3228
BaeGI GKGCMC 2 cut(s) 1585, 3099
BaeI ACNNNNGTAYC 2 cut(s) 1167, 1200
BalI TGGCCA 1 cut(s) 3069
BamHI GGATCC 1 cut(s) 2626
BanI GGYRCC 1 cut(s) 1767
BanII GRGCYC 3 cut(s) 1585, 3099, 3707
BauI CACGAG 1 cut(s) 3756
BbsI GAAGAC 2 cut(s) 18, 2417
Bbv12I GWGCWC 2 cut(s) 1026, 3707
BbvI GCAGC 9 cut(s) 403, 425, 711, 1189, 2080, 2124, 2548, 3305, 3426
BccI CCATC 8 cut(s) 433, 1112, 1118, 1334, 1339, 1934, 3181, 3231
BcgI CGANNNNNNTGC 2 cut(s) 2904, 2938
BciT130I CCWGG 7 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978
BclI TGATCA 3 cut(s) 169, 2671, 2784
BcnI CCSGG 1 cut(s) 3873
BcoDI GTCTC 6 cut(s) 660, 1319, 1410, 1601, 2883, 3300
BfmI CTRYAG 5 cut(s) 204, 873, 1266, 1335, 1805
BfoI RGCGCY 2 cut(s) 322, 2680
BfrI CTTAAG 1 cut(s) 2690
BfuAI ACCTGC 1 cut(s) 3198
BglII AGATCT 1 cut(s) 300
Bme1390I CCNGG 8 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978, 3873
Bme18I GGWCC 3 cut(s) 271, 1141, 2974
BmeT110I CYCGRG 3 cut(s) 831, 967, 2585
BmgBI CACGTC 3 cut(s) 1138, 1420, 2152
BmgT120I GGNCC 8 cut(s) 271, 884, 1141, 1581, 1582, 2974, 3095, 3096
BmiI GGNNCC 7 cut(s) 1285, 1583, 1584, 1769, 2628, 3097, 3402
BmrFI CCNGG 8 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978, 3873
BmrI ACTGGG 2 cut(s) 158, 2041
BmuI ACTGGG 2 cut(s) 158, 2041
BpiI GAAGAC 2 cut(s) 18, 2417
BpmI CTGGAG 4 cut(s) 375, 2334, 3349, 3419
Bpu14I TTCGAA 1 cut(s) 1484
BpuEI CTTGAG 3 cut(s) 810, 1697, 3787
BpuMI CCSGG 1 cut(s) 3873
Bsa29I ATCGAT 1 cut(s) 1611
BsaAI YACGTR 1 cut(s) 2879
BsaHI GRCGYC 1 cut(s) 1901
BsaI GGTCTC 2 cut(s) 1319, 1601
BsaJI CCNNGG 7 cut(s) 495, 1220, 1821, 1822, 2429, 3380, 3698
BsaXI ACNNNNNCTCC 2 cut(s) 2307, 2337
Bsc4I CCNNNNNNNGG 7 cut(s) 482, 943, 1194, 1592, 2032, 2741, 3229
Bse21I CCTNAGG 1 cut(s) 3228
Bse3DI GCAATG 3 cut(s) 476, 512, 1879
BseBI CCWGG 7 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978
BseCI ATCGAT 1 cut(s) 1611
BseDI CCNNGG 7 cut(s) 495, 1220, 1821, 1822, 2429, 3380, 3698
BseLI CCNNNNNNNGG 7 cut(s) 482, 943, 1194, 1592, 2032, 2741, 3229
BseMI GCAATG 3 cut(s) 476, 512, 1879
BseMII CTCAG 8 cut(s) 1261, 1506, 1752, 2133, 2439, 2609, 3242, 3495
BseRI GAGGAG 2 cut(s) 1583, 3530
BseSI GKGCMC 2 cut(s) 1585, 3099
BseXI GCAGC 9 cut(s) 403, 425, 711, 1189, 2080, 2124, 2548, 3305, 3426
BseYI CCCAGC 1 cut(s) 1911
BsgI GTGCAG 4 cut(s) 718, 1713, 2965, 3179
Bsh1236I CGCG 1 cut(s) 3688
BshFI GGCC 7 cut(s) 743, 886, 1583, 1791, 2993, 3069, 3097
BshNI GGYRCC 1 cut(s) 1767
BshVI ATCGAT 1 cut(s) 1611
BsiHKAI GWGCWC 2 cut(s) 1026, 3707
BsiHKCI CYCGRG 3 cut(s) 831, 967, 2585
BsiSI CCGG 2 cut(s) 1188, 3872
BslFI GGGAC 1 cut(s) 2048
BslI CCNNNNNNNGG 7 cut(s) 482, 943, 1194, 1592, 2032, 2741, 3229
BsmAI GTCTC 6 cut(s) 660, 1319, 1410, 1601, 2883, 3300
BsmBI CGTCTC 3 cut(s) 660, 1410, 2883
BsmFI GGGAC 1 cut(s) 2048
BsmI GAATGC 5 cut(s) 339, 1043, 1723, 2736, 3472
BsnI GGCC 7 cut(s) 743, 886, 1583, 1791, 2993, 3069, 3097
Bso31I GGTCTC 2 cut(s) 1319, 1601
BsoBI CYCGRG 3 cut(s) 831, 967, 2585
Bsp119I TTCGAA 1 cut(s) 1484
Bsp120I GGGCCC 2 cut(s) 1581, 3095
Bsp1286I GDGCHC 4 cut(s) 1026, 1585, 3099, 3707
Bsp1407I TGTACA 2 cut(s) 1743, 2899
Bsp19I CCATGG 1 cut(s) 3380
Bsp68I TCGCGA 1 cut(s) 3688
BspACI CCGC 6 cut(s) 702, 1401, 1922, 1962, 3149, 3321
BspANI GGCC 7 cut(s) 743, 886, 1583, 1791, 2993, 3069, 3097
BspCNI CTCAG 8 cut(s) 1260, 1505, 1753, 2132, 2440, 2610, 3241, 3496
BspDI ATCGAT 1 cut(s) 1611
BspFNI CGCG 1 cut(s) 3688
BspHI TCATGA 2 cut(s) 2467, 2499
BspLI GGNNCC 7 cut(s) 1285, 1583, 1584, 1769, 2628, 3097, 3402
BspMAI CTGCAG 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 3198
BspT104I TTCGAA 1 cut(s) 1484
BspT107I GGYRCC 1 cut(s) 1767
BspTI CTTAAG 1 cut(s) 2690
BspTNI GGTCTC 2 cut(s) 1319, 1601
BsrDI GCAATG 3 cut(s) 476, 512, 1879
BsrGI TGTACA 2 cut(s) 1743, 2899
BssECI CCNNGG 7 cut(s) 495, 1220, 1821, 1822, 2429, 3380, 3698
BssNI GRCGYC 1 cut(s) 1901
BssSI CACGAG 1 cut(s) 3756
BssT1I CCWWGG 5 cut(s) 495, 1220, 2429, 3380, 3698
Bst2BI CACGAG 1 cut(s) 3756
Bst2UI CCWGG 7 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978
Bst6I CTCTTC 2 cut(s) 859, 3643
BstACI GRCGYC 1 cut(s) 1901
BstAFI CTTAAG 1 cut(s) 2690
BstAUI TGTACA 2 cut(s) 1743, 2899
BstBAI YACGTR 1 cut(s) 2879
BstBI TTCGAA 1 cut(s) 1484
BstC8I GCNNGC 7 cut(s) 1013, 1360, 1399, 2808, 3067, 3444, 3583
BstDSI CCRYGG 1 cut(s) 3380
BstEII GGTNACC 1 cut(s) 3131
BstENI CCTNNNNNAGG 2 cut(s) 480, 2739
BstFNI CGCG 1 cut(s) 3688
BstH2I RGCGCY 2 cut(s) 322, 2680
BstHHI GCGC 5 cut(s) 321, 872, 1043, 2092, 2679
BstMAI GTCTC 6 cut(s) 660, 1319, 1410, 1601, 2883, 3300
BstNI CCWGG 7 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978
BstNSI RCATGY 4 cut(s) 1401, 1991, 3003, 3599
BstPI GGTNACC 1 cut(s) 3131
BstSCI CCNGG 8 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976, 3871
BstSFI CTRYAG 5 cut(s) 204, 873, 1266, 1335, 1805
BstSLI GKGCMC 2 cut(s) 1585, 3099
BstSNI TACGTA 1 cut(s) 2879
BstUI CGCG 1 cut(s) 3688
BstV1I GCAGC 9 cut(s) 403, 425, 711, 1189, 2080, 2124, 2548, 3305, 3426
BstV2I GAAGAC 2 cut(s) 18, 2417
BstX2I RGATCY 6 cut(s) 300, 713, 1250, 2581, 2609, 2626
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 6 cut(s) 300, 713, 1250, 2581, 2609, 2626
Bsu15I ATCGAT 1 cut(s) 1611
Bsu36I CCTNAGG 1 cut(s) 3228
BsuRI GGCC 7 cut(s) 743, 886, 1583, 1791, 2993, 3069, 3097
BsuTUI ATCGAT 1 cut(s) 1611
BtgI CCRYGG 1 cut(s) 3380
BtgZI GCGATG 1 cut(s) 763
BtrI CACGTC 3 cut(s) 1138, 1420, 2152
BtsI GCAGTG 5 cut(s) 153, 201, 2700, 2953, 3716
BtuMI TCGCGA 1 cut(s) 3688
BveI ACCTGC 1 cut(s) 3198
Cac8I GCNNGC 7 cut(s) 1013, 1360, 1399, 2808, 3067, 3444, 3583
CaiI CAGNNNCTG 1 cut(s) 1918
CciI TCATGA 2 cut(s) 2467, 2499
CfoI GCGC 5 cut(s) 321, 872, 1043, 2092, 2679
Cfr13I GGNCC 8 cut(s) 271, 884, 1141, 1581, 1582, 2974, 3095, 3096
ClaI ATCGAT 1 cut(s) 1611
CseI GACGC 1 cut(s) 1890
CsiI ACCWGGT 1 cut(s) 2976
CspCI CAANNNNNGTGG 2 cut(s) 3597, 3632
DraI TTTAAA 3 cut(s) 1438, 1684, 3882
DrdI GACNNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 3067
Eam1104I CTCTTC 2 cut(s) 859, 3643
EarI CTCTTC 2 cut(s) 859, 3643
Ecl136II GAGCTC 1 cut(s) 3705
Eco105I TACGTA 1 cut(s) 2879
Eco130I CCWWGG 5 cut(s) 495, 1220, 2429, 3380, 3698
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 3 cut(s) 1585, 3099, 3707
Eco31I GGTCTC 2 cut(s) 1319, 1601
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 3 cut(s) 271, 1141, 2974
Eco47III AGCGCT 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 3705
Eco57I CTGAAG 5 cut(s) 12, 39, 335, 1793, 3351
Eco81I CCTNAGG 1 cut(s) 3228
Eco88I CYCGRG 3 cut(s) 831, 967, 2585
Eco91I GGTNACC 1 cut(s) 3131
EcoICRI GAGCTC 1 cut(s) 3705
EcoNI CCTNNNNNAGG 2 cut(s) 480, 2739
EcoO109I RGGNCCY 3 cut(s) 1582, 3095, 3096
EcoO65I GGTNACC 1 cut(s) 3131
EcoRI GAATTC 2 cut(s) 910, 2495
EcoRII CCWGG 7 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 5 cut(s) 495, 1220, 2429, 3380, 3698
EcoT22I ATGCAT 2 cut(s) 1399, 3053
EcoT38I GRGCYC 3 cut(s) 1585, 3099, 3707
ErhI CCWWGG 5 cut(s) 495, 1220, 2429, 3380, 3698
Esp3I CGTCTC 3 cut(s) 660, 1410, 2883
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 2673, 2705
FaqI GGGAC 1 cut(s) 2048
FbaI TGATCA 3 cut(s) 169, 2671, 2784
FriOI GRGCYC 3 cut(s) 1585, 3099, 3707
FspI TGCGCA 1 cut(s) 2091
GlaI GCGC 5 cut(s) 320, 871, 1042, 2091, 2678
GsaI CCCAGC 1 cut(s) 1915
GsuI CTGGAG 4 cut(s) 375, 2334, 3349, 3419
HaeII RGCGCY 2 cut(s) 322, 2680
HaeIII GGCC 7 cut(s) 743, 886, 1583, 1791, 2993, 3069, 3097
HapII CCGG 2 cut(s) 1188, 3872
HgaI GACGC 1 cut(s) 1890
HhaI GCGC 5 cut(s) 321, 872, 1043, 2092, 2679
Hin1I GRCGYC 1 cut(s) 1901
Hin6I GCGC 5 cut(s) 319, 870, 1041, 2090, 2677
HinP1I GCGC 5 cut(s) 319, 870, 1041, 2090, 2677
HincII GTYRAC 3 cut(s) 613, 1905, 3526
HindII GTYRAC 3 cut(s) 613, 1905, 3526
HindIII AAGCTT 5 cut(s) 531, 677, 2775, 3196, 3489
HpaI GTTAAC 2 cut(s) 613, 3526
HpaII CCGG 2 cut(s) 1188, 3872
HphI GGTGA 6 cut(s) 380, 510, 1074, 1747, 1982, 3143
Hpy166II GTNNAC 7 cut(s) 613, 1141, 1746, 1905, 2817, 2902, 3526
Hpy8I GTNNAC 7 cut(s) 613, 1141, 1746, 1905, 2817, 2902, 3526
Hpy99I CGWCG 1 cut(s) 1872
HpyAV CCTTC 9 cut(s) 87, 116, 594, 875, 1406, 1480, 1802, 2048, 2451
HpyCH4IV ACGT 9 cut(s) 695, 961, 1137, 1419, 2151, 2215, 2422, 2878, 3744
HpySE526I ACGT 9 cut(s) 695, 961, 1137, 1419, 2151, 2215, 2422, 2878, 3744
Hsp92I GRCGYC 1 cut(s) 1901
HspAI GCGC 5 cut(s) 319, 870, 1041, 2090, 2677
KpnI GGTACC 1 cut(s) 1771
Ksp22I TGATCA 3 cut(s) 169, 2671, 2784
KspAI GTTAAC 2 cut(s) 613, 3526
LmnI GCTCC 8 cut(s) 704, 1702, 1755, 2433, 3400, 3517, 3702, 3844
Lsp1109I GCAGC 9 cut(s) 403, 425, 711, 1189, 2080, 2124, 2548, 3305, 3426
MabI ACCWGGT 1 cut(s) 2976
MaeII ACGT 9 cut(s) 695, 961, 1137, 1419, 2151, 2215, 2422, 2878, 3744
MaeIII GTNAC 8 cut(s) 69, 691, 1133, 1929, 2152, 2245, 3131, 3243
MflI RGATCY 6 cut(s) 300, 713, 1250, 2581, 2609, 2626
MhlI GDGCHC 4 cut(s) 1026, 1585, 3099, 3707
MlsI TGGCCA 1 cut(s) 3069
MluNI TGGCCA 1 cut(s) 3069
MlyI GAGTC 3 cut(s) 266, 1484, 2668
MmeI TCCRAC 4 cut(s) 162, 2050, 2952, 3629
Mox20I TGGCCA 1 cut(s) 3069
Mph1103I ATGCAT 2 cut(s) 1399, 3053
MroXI GAANNNNTTC 3 cut(s) 320, 405, 2910
MscI TGGCCA 1 cut(s) 3069
MslI CAYNNNNRTG 5 cut(s) 1031, 1895, 2097, 2939, 3350
Msp20I TGGCCA 1 cut(s) 3069
MspA1I CMGCKG 2 cut(s) 416, 702
MspCI CTTAAG 1 cut(s) 2690
MspI CCGG 2 cut(s) 1188, 3872
MspR9I CCNGG 8 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978, 3873
Mva1269I GAATGC 5 cut(s) 339, 1043, 1723, 2736, 3472
MvaI CCWGG 7 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978
MvnI CGCG 1 cut(s) 3688
NciI CCSGG 1 cut(s) 3873
NcoI CCATGG 1 cut(s) 3380
NlaIV GGNNCC 7 cut(s) 1285, 1583, 1584, 1769, 2628, 3097, 3402
NmuCI GTSAC 4 cut(s) 69, 1133, 2152, 3131
NruI TCGCGA 1 cut(s) 3688
NsbI TGCGCA 1 cut(s) 2091
NsiI ATGCAT 2 cut(s) 1399, 3053
NspI RCATGY 4 cut(s) 1401, 1991, 3003, 3599
NspV TTCGAA 1 cut(s) 1484
OliI CACNNNNGTG 2 cut(s) 1031, 3350
PaeI GCATGC 1 cut(s) 1401
PaeR7I CTCGAG 2 cut(s) 831, 2585
PagI TCATGA 2 cut(s) 2467, 2499
PasI CCCWGGG 1 cut(s) 1822
PceI AGGCCT 1 cut(s) 743
PciI ACATGT 2 cut(s) 1987, 2999
PcsI WCGNNNNNNNCGW 1 cut(s) 2212
PctI GAATGC 5 cut(s) 339, 1043, 1723, 2736, 3472
PdmI GAANNNNTTC 3 cut(s) 320, 405, 2910
PfeI GAWTC 9 cut(s) 1091, 1325, 1637, 2196, 2231, 2326, 3009, 3555, 3862
PflFI GACNNNGTC 1 cut(s) 2978
PfoI TCCNGGA 1 cut(s) 328
PleI GAGTC 3 cut(s) 265, 1484, 2667
PpsI GAGTC 3 cut(s) 265, 1484, 2667
Ppu21I YACGTR 1 cut(s) 2879
PscI ACATGT 2 cut(s) 1987, 2999
PsiI TTATAA 2 cut(s) 999, 3570
Psp124BI GAGCTC 1 cut(s) 3707
Psp1406I AACGTT 1 cut(s) 3744
Psp6I CCWGG 7 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976
PspEI GGTNACC 1 cut(s) 3131
PspFI CCCAGC 1 cut(s) 1911
PspGI CCWGG 7 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976
PspN4I GGNNCC 7 cut(s) 1285, 1583, 1584, 1769, 2628, 3097, 3402
PspOMI GGGCCC 2 cut(s) 1581, 3095
PspPI GGNCC 8 cut(s) 271, 884, 1141, 1581, 1582, 2974, 3095, 3096
PspXI VCTCGAGB 1 cut(s) 831
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 1918
PsuI RGATCY 6 cut(s) 300, 713, 1250, 2581, 2609, 2626
PsyI GACNNNGTC 1 cut(s) 2978
PvuII CAGCTG 1 cut(s) 416
RruI TCGCGA 1 cut(s) 3688
RseI CAYNNNNRTG 5 cut(s) 1031, 1895, 2097, 2939, 3350
SacI GAGCTC 1 cut(s) 3707
Sau96I GGNCC 8 cut(s) 271, 884, 1141, 1581, 1582, 2974, 3095, 3096
SchI GAGTC 3 cut(s) 266, 1484, 2668
ScrFI CCNGG 8 cut(s) 330, 1086, 1823, 2294, 2624, 2736, 2978, 3873
SduI GDGCHC 4 cut(s) 1026, 1585, 3099, 3707
SexAI ACCWGGT 1 cut(s) 2976
SfcI CTRYAG 5 cut(s) 204, 873, 1266, 1335, 1805
Sfr274I CTCGAG 2 cut(s) 831, 2585
SfuI TTCGAA 1 cut(s) 1484
SinI GGWCC 3 cut(s) 271, 1141, 2974
SlaI CTCGAG 2 cut(s) 831, 2585
SmiMI CAYNNNNRTG 5 cut(s) 1031, 1895, 2097, 2939, 3350
SmlI CTYRAG 6 cut(s) 825, 831, 1676, 2585, 2690, 3802
SmoI CTYRAG 6 cut(s) 825, 831, 1676, 2585, 2690, 3802
SnaBI TACGTA 1 cut(s) 2879
SphI GCATGC 1 cut(s) 1401
SseBI AGGCCT 1 cut(s) 743
SsiI CCGC 6 cut(s) 702, 1401, 1922, 1962, 3149, 3321
SstI GAGCTC 1 cut(s) 3707
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 8 cut(s) 328, 1084, 1821, 2292, 2622, 2734, 2976, 3871
StyI CCWWGG 5 cut(s) 495, 1220, 2429, 3380, 3698
TaiI ACGT 9 cut(s) 698, 964, 1140, 1422, 2154, 2218, 2425, 2881, 3747
TaqII GACCGA 1 cut(s) 1609
TatI WGTACW 6 cut(s) 686, 1743, 1797, 2899, 3121, 3164
TauI GCSGC 1 cut(s) 1924
TfiI GAWTC 9 cut(s) 1091, 1325, 1637, 2196, 2231, 2326, 3009, 3555, 3862
TseFI GTSAC 4 cut(s) 69, 1133, 2152, 3131
TseI GCWGC 9 cut(s) 413, 416, 699, 1202, 2068, 2112, 2536, 3318, 3439
Tsp45I GTSAC 4 cut(s) 69, 1133, 2152, 3131
TspGWI ACGGA 2 cut(s) 299, 2092
Tth111I GACNNNGTC 1 cut(s) 2978
Vha464I CTTAAG 1 cut(s) 2690
VpaK11BI GGWCC 3 cut(s) 271, 1141, 2974
XagI CCTNNNNNAGG 2 cut(s) 480, 2739
XapI RAATTY 5 cut(s) 454, 910, 1827, 2481, 2495
XbaI TCTAGA 1 cut(s) 2234
XceI RCATGY 4 cut(s) 1401, 1991, 3003, 3599
XhoI CTCGAG 2 cut(s) 831, 2585
XmnI GAANNNNTTC 3 cut(s) 320, 405, 2910
Zsp2I ATGCAT 2 cut(s) 1399, 3053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.