Rorug01G0165900

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002244
Physical Location & Seq
Reverse (-)
22636535 .. 22641113
4579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0165900.1

Sequence Viewer

Length: 351 bp
ATGGGTTCCAAGGATCCTGCTCCGGTGGTTGACAACGTTAAGAGGGCAAAGCAGGTGCCGGTGGACTGGTCTTGTGTGAAAATTAAAGTCGGACAGTATCAAGGGGGAATTGTACTTGGTATTAATAAATTGGAGGATAGATCTGTGTCTCGAACTAGCACGTCATGCACAAGCCAAAGCTCTTTTTGCAAGGACTCTTTTGGGTCATCGCCTTTTTCACTCTTCTGCACACTCTCTCTCTCCAAAGCCCCAAACCCTCACTTCCCTGCTCAATCATTGCAGATTGGCATAGAGAGAGAGCTTGAACAACAACTTCAACTTCAGCTGTTACTTTTTAAGTTTGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

116

Amino Acids

12.72

Weight (kDa)

8.76

Isoelectric Point (pI)

71.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 55
AclI AACGTT 1 cut(s) 36
AclWI GGATC 2 cut(s) 8, 21
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 1 cut(s) 114
AgsI TTSAA 2 cut(s) 305, 317
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 3 cut(s) 180, 301, 325
AluI AGCT 3 cut(s) 180, 301, 325
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 8, 21
AseI ATTAAT 1 cut(s) 123
BamHI GGATCC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 55
BccI CCATC 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 7, 15, 57
BsaJI CCNNGG 1 cut(s) 9
BsaWI WCCGGW 1 cut(s) 22
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 9
BseMI GCAATG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 71
BsgI GTGCAG 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 2 cut(s) 23, 59
BsmAI GTCTC 1 cut(s) 153
Bsp143I GATC 2 cut(s) 13, 140
BspLI GGNNCC 3 cut(s) 7, 15, 57
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 8, 21
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 2 cut(s) 13, 140
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 96
Bst6I CTCTTC 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 165
BstKTI GATC 2 cut(s) 16, 143
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 2 cut(s) 13, 140
BstMWI GCNNNNNNNGC 2 cut(s) 165, 186
BstX2I RGATCY 2 cut(s) 13, 140
BstYI RGATCY 2 cut(s) 13, 140
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 162
BveI ACCTGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 58
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 165
CviJI RGCY 5 cut(s) 174, 180, 248, 301, 325
CviKI_1 RGCY 5 cut(s) 174, 180, 248, 301, 325
CviQI GTAC 1 cut(s) 113
DpnI GATC 2 cut(s) 15, 142
DpnII GATC 2 cut(s) 13, 140
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 168
FaiI YATR 2 cut(s) 166, 290
FatI CATG 1 cut(s) 164
FspBI CTAG 1 cut(s) 156
HapII CCGG 2 cut(s) 23, 59
Hin1II CATG 1 cut(s) 168
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 1 cut(s) 194
HpaII CCGG 2 cut(s) 23, 59
Hpy166II GTNNAC 2 cut(s) 31, 64
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 2 cut(s) 31, 64
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4IV ACGT 2 cut(s) 36, 161
HpyCH4V TGCA 4 cut(s) 168, 189, 228, 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 186
HpySE526I ACGT 2 cut(s) 36, 161
Hsp92II CATG 1 cut(s) 168
Kzo9I GATC 2 cut(s) 13, 140
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 6 cut(s) 30, 36, 38, 52, 72, 279
MaeI CTAG 1 cut(s) 156
MaeII ACGT 2 cut(s) 36, 161
MaeIII GTNAC 1 cut(s) 327
MalI GATC 2 cut(s) 15, 142
MboI GATC 2 cut(s) 13, 140
MboII GAAGA 1 cut(s) 214
MflI RGATCY 2 cut(s) 13, 140
MluCI AATT 3 cut(s) 81, 108, 128
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 3 cut(s) 36, 127, 267
MseI TTAA 4 cut(s) 39, 84, 123, 336
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 2 cut(s) 23, 59
MwoI GCNNNNNNNGC 2 cut(s) 165, 186
NdeII GATC 2 cut(s) 13, 140
NlaIII CATG 1 cut(s) 168
NlaIV GGNNCC 3 cut(s) 7, 15, 57
PaqCI CACCTGC 1 cut(s) 43
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 7, 15, 57
PsuI RGATCY 2 cut(s) 13, 140
PvuII CAGCTG 1 cut(s) 325
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 4 cut(s) 39, 84, 123, 336
Sau3AI GATC 2 cut(s) 13, 140
SchI GAGTC 1 cut(s) 188
SetI ASST 6 cut(s) 39, 57, 164, 182, 303, 327
Sse9I AATT 3 cut(s) 81, 108, 128
SspMI CTAG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 96
TaiI ACGT 2 cut(s) 39, 164
TaqI TCGA 1 cut(s) 151
TasI AATT 3 cut(s) 81, 108, 128
TatI WGTACW 1 cut(s) 112
Tru1I TTAA 4 cut(s) 39, 84, 123, 336
Tru9I TTAA 4 cut(s) 39, 84, 123, 336
VspI ATTAAT 1 cut(s) 123
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.