RchiOBHm_Chr2g0122971

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002244
Physical Location & Seq
Forward (+)
36090755 .. 36092998
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49531

Sequence Viewer

Length: 714 bp
ATGCAAAGAATGGGGATCCAGAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCACAAAAATATTAAATTGGGTTAACATCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAAGTCGCAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTTGAAGCTAACAAAAATGAACCTGACGTGAAGGTTATGCCTTCGGAGTTTAAAACCGGGAAAGTGCCAAAGGACCAGGCAAGACTATTCTCTGTACCAAAATCTCTTAGGGGATTATTATACTCAACTGATGAGCATAGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAAAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACATTGTTGGGCTAATCGTGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTGCGGAATTCAAGCTTGTGCAGAATTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGTTGCAGAACAAATCCGAAACGGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

237

Amino Acids

26.74

Weight (kDa)

8.72

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 6 - 92 8.1e-21 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 459
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 1 cut(s) 69
AciI CCGC 2 cut(s) 524, 625
AclI AACGTT 1 cut(s) 77
AclWI GGATC 3 cut(s) 10, 23, 172
AcsI RAATTY 2 cut(s) 628, 645
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 375, 461
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 3 cut(s) 124, 284, 633
AhdI GACNNNNNGTC 1 cut(s) 605
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 2 cut(s) 132, 354
AluBI AGCT 3 cut(s) 146, 287, 636
AluI AGCT 3 cut(s) 146, 287, 636
Alw21I GWGCWC 1 cut(s) 671
Alw44I GTGCAC 1 cut(s) 667
AlwI GGATC 3 cut(s) 10, 23, 172
AoxI GGCC 1 cut(s) 591
ApaLI GTGCAC 1 cut(s) 667
ApoI RAATTY 2 cut(s) 628, 645
Asp718I GGTACC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 190, 286
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 2 cut(s) 424, 671
BamHI GGATCC 1 cut(s) 15
BanI GGYRCC 1 cut(s) 459
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 671
BccI CCATC 3 cut(s) 214, 663, 680
BciT130I CCWGG 2 cut(s) 134, 356
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 337
BfaI CTAG 1 cut(s) 712
BglII AGATCT 1 cut(s) 533
Bme1390I CCNGG 3 cut(s) 134, 337, 356
Bme18I GGWCC 1 cut(s) 352
BmeRI GACNNNNNGTC 1 cut(s) 605
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 3 cut(s) 17, 231, 461
BmrFI CCNGG 3 cut(s) 134, 337, 356
BmsI GCATC 2 cut(s) 76, 250
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 580
BpuMI CCSGG 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 2 cut(s) 484, 499
BseBI CCWGG 2 cut(s) 134, 356
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 2 cut(s) 159, 189
BseNI ACTGG 2 cut(s) 484, 499
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 2 cut(s) 424, 671
BsgI GTGCAG 1 cut(s) 661
BshFI GGCC 1 cut(s) 593
BshNI GGYRCC 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 239
BsnI GGCC 1 cut(s) 593
Bsp1286I GDGCHC 3 cut(s) 232, 424, 671
Bsp143I GATC 4 cut(s) 15, 164, 505, 533
BspACI CCGC 2 cut(s) 524, 625
BspANI GGCC 1 cut(s) 593
BspCNI CTCAG 2 cut(s) 160, 190
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 3 cut(s) 17, 231, 461
BspPI GGATC 3 cut(s) 10, 23, 172
BspT107I GGYRCC 1 cut(s) 459
BsrI ACTGG 2 cut(s) 484, 499
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 4 cut(s) 15, 164, 505, 533
BssNAI GTATAC 1 cut(s) 70
Bst1107I GTATAC 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 134, 356
Bst6I CTCTTC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 168, 198, 386
BstKTI GATC 4 cut(s) 18, 167, 508, 536
BstMBI GATC 4 cut(s) 15, 164, 505, 533
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 2 cut(s) 134, 356
BstNSI RCATGY 2 cut(s) 48, 473
BstSCI CCNGG 3 cut(s) 132, 335, 354
BstSLI GKGCMC 2 cut(s) 424, 671
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 3 cut(s) 15, 164, 533
BstYI RGATCY 3 cut(s) 15, 164, 533
BstZ17I GTATAC 1 cut(s) 70
BsuRI GGCC 1 cut(s) 593
BtrI CACGTC 1 cut(s) 307
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 374, 460
CviAII CATG 4 cut(s) 45, 56, 225, 470
CviJI RGCY 7 cut(s) 146, 230, 287, 550, 593, 636, 711
CviKI_1 RGCY 7 cut(s) 146, 230, 287, 550, 593, 636, 711
CviQI GTAC 2 cut(s) 374, 460
DdeI CTNAG 3 cut(s) 168, 198, 386
DpnI GATC 4 cut(s) 17, 166, 507, 535
DpnII GATC 4 cut(s) 15, 164, 505, 533
DraI TTTAAA 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 605
Eam1104I CTCTTC 1 cut(s) 447
Eam1105I GACNNNNNGTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 2 cut(s) 132, 354
EcoT22I ATGCAT 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 232
FaeI CATG 4 cut(s) 48, 59, 228, 473
FatI CATG 4 cut(s) 44, 55, 224, 469
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 1 cut(s) 69
FriOI GRGCYC 1 cut(s) 232
FspBI CTAG 1 cut(s) 712
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 593
HapII CCGG 1 cut(s) 336
Hin1II CATG 4 cut(s) 48, 59, 228, 473
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 2 cut(s) 144, 634
HinfI GANTC 1 cut(s) 137
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 1 cut(s) 336
HphI GGTGA 2 cut(s) 190, 286
Hpy166II GTNNAC 3 cut(s) 70, 115, 669
Hpy188III TCNNGA 3 cut(s) 19, 56, 597
Hpy8I GTNNAC 3 cut(s) 70, 115, 669
HpyAV CCTTC 4 cut(s) 304, 330, 620, 701
HpyCH4IV ACGT 3 cut(s) 77, 306, 562
HpyCH4V TGCA 8 cut(s) 4, 44, 559, 575, 604, 642, 669, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 3 cut(s) 168, 198, 386
HpySE526I ACGT 3 cut(s) 77, 306, 562
Hsp92II CATG 4 cut(s) 48, 59, 228, 473
KpnI GGTACC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 505
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 15, 164, 505, 533
LmnI GCTCC 1 cut(s) 235
LweI GCATC 2 cut(s) 76, 250
MaeI CTAG 1 cut(s) 712
MaeII ACGT 3 cut(s) 77, 306, 562
MaeIII GTNAC 2 cut(s) 91, 491
MalI GATC 4 cut(s) 17, 166, 507, 535
MboI GATC 4 cut(s) 15, 164, 505, 533
MboII GAAGA 4 cut(s) 112, 434, 476, 543
MflI RGATCY 3 cut(s) 15, 164, 533
MhlI GDGCHC 3 cut(s) 232, 424, 671
MluCI AATT 5 cut(s) 106, 204, 519, 628, 645
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 5 cut(s) 193, 208, 450, 506, 604
Mph1103I ATGCAT 1 cut(s) 46
MseI TTAA 3 cut(s) 104, 114, 330
MslI CAYNNNNRTG 2 cut(s) 60, 660
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 3 cut(s) 134, 337, 356
MvaI CCWGG 2 cut(s) 134, 356
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 1 cut(s) 337
NdeII GATC 4 cut(s) 15, 164, 505, 533
NlaIII CATG 4 cut(s) 48, 59, 228, 473
NlaIV GGNNCC 3 cut(s) 17, 231, 461
NmuCI GTSAC 1 cut(s) 91
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 2 cut(s) 48, 473
PagI TCATGA 1 cut(s) 55
PceI AGGCCT 1 cut(s) 593
PfeI GAWTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 132, 354
PspGI CCWGG 2 cut(s) 132, 354
PspN4I GGNNCC 3 cut(s) 17, 231, 461
PspPI GGNCC 1 cut(s) 352
PsuI RGATCY 3 cut(s) 15, 164, 533
RsaI GTAC 2 cut(s) 375, 461
RsaNI GTAC 2 cut(s) 374, 460
RseI CAYNNNNRTG 2 cut(s) 60, 660
SaqAI TTAA 3 cut(s) 104, 114, 330
Sau3AI GATC 4 cut(s) 15, 164, 505, 533
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 3 cut(s) 134, 337, 356
SduI GDGCHC 3 cut(s) 232, 424, 671
SfaNI GCATC 2 cut(s) 76, 250
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 60, 660
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
Sse9I AATT 5 cut(s) 106, 204, 519, 628, 645
SseBI AGGCCT 1 cut(s) 593
SsiI CCGC 2 cut(s) 524, 625
SspI AATATT 1 cut(s) 102
SspMI CTAG 1 cut(s) 712
StuI AGGCCT 1 cut(s) 593
StyD4I CCNGG 3 cut(s) 132, 335, 354
TaiI ACGT 3 cut(s) 80, 309, 565
TasI AATT 5 cut(s) 106, 204, 519, 628, 645
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 3 cut(s) 104, 114, 330
Tru9I TTAA 3 cut(s) 104, 114, 330
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 4 cut(s) 44, 72, 138, 312
Van91I CCANNNNNTGG 1 cut(s) 239
VneI GTGCAC 1 cut(s) 667
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 2 cut(s) 628, 645
XceI RCATGY 2 cut(s) 48, 473
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.