RLG00000028987

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002244
Physical Location & Seq
Forward (+)
31111442 .. 31123432
11991 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028987

Sequence Viewer

Length: 4104 bp
ATGGCTCCTAGAAAGAAACCCAATTCTTCTTCTTCTTCTTCTTCTACCGCTTCGTCTCGGAAATCGAACTCGAAAGCTCAGCCTCAGCCTTCCAAGTTCAGCATCCAACATTTCTTTGACCGCCACACCCAGAACTCTCAAAACCCCCAAACCACCACCTCTTCCACTCCTCACAACCCTAAGCCCCCAATCCCTGACTCTTCCACCGCCATTGTTCCGCAGAAGTCGATTCCGGAGATCCAAAACCCTGCCCCGATTGAAGCAAATAGTGCACTAGTAGCTTCTGGAAATCCCGAGAACCGGCGTGATTCGCAGTCGAGTAATAAGAACAGTACGCAGGTGACACCAACTGAGAATTTGGTGGCGGTGAGGTTTATGGATGCCGAGCCGAATGTGGATGAGGAGGTCTCACCGGAGGTTTCGAAGTCTAAGTCTGTCAAGCGATTCAAGTTCTCCCCTGGAATGTTGATAAAGCAGAGTCAAGACGATGGAGGTGATGAGGTGACGTGGAGGACGTCTCCGGTTAATGAAAGACTTAAAGCAGTTTCAAAGCACAAGCCTGAGATGATGAGAGTGTTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGCAAAACAAGACTTCTGCTGTTGAAGCCGGAAAGGTTGAGAAATCCCTGCCGTTGGTGTCCCTGAATAGGGTTGGCTTGAAAAGGGTTAAACCGGATCAGGATGTGGATCATAATGGAAATGTGGTCGATAGGACTATTTCTGAAATAGCTGGTAGTCAGAGTCCATTCCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTGCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATTTTTGTTGAAGATTCAGTACCTGATACTGTGGAGGCATCTCAATTACAAGTTAAAGATAAATCAAAGCATGTCGTAAAAAGAGTGGTGACAGCTCCAACAGAACAGGTTACTCGGGCATCTTCCAATTACAGTTTCCTTGTATTGGAGGTATCTGAGAATTGTAGACCTGATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTATGGGAAGAGTGGTTTTACAGTGTCATTGCACCTGGAGATACTGTCAACGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATCATCACCGTAACTTTTTAATTGTTCACCCTGATGTCTTAGTGTCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCCTAGGCGAACTGTGCTGGATGAAAGGCTAAAAGGCAATGAGTATTCAACTCCAGCACTAATTGGCACAATACTGCATCAAGTTTTTGAGGCTGCACTTATGAAGGAGAAACCCACAATTACTTTCTTGGAGGAACATGCAAGAATGGGGCTCCAGAGAAGCATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTGAACGTCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGAATTTGGATCTGAGAATGGGGTGAAAAAAGTCACAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTCGAAGCTAACAAAAATGAATCTGACATGAAGGTTATGCCTTTGGAGTTTAAAACCGGGAAAGAGCCAAAGGACCAGGAGCATTGTGCCCAAGTGATCTTATACACTCTTCTTATGTCGGAGAGGTACCAGAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACAAAGGGAATTGCAGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACGGAGTCCAAGCTTGTGCAGAAGTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGGCACATGGAGGCAACGCAGATAGCAGCGGATTGGAAGGTTTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGACTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGGTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGCGATCATTCCACAAGTTGCCTTTCTTCTATGGTTCTTGATAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTACTGGTTTGTCTTCTCTCAATATGAGGACATCCAATGGAGATTTTCAGAATGCTGCTTCTTCACCCTCAAAATTTGCTGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTGGCTGTTGCAAGGGGGGTCATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGGGCAATCCTTCTTCAGAGGCAAGTGATCTTCGTCGGGAGGTCTGGCGGGTTGACAAAGATGAATTTATGACATCATTTGCCACAATGAGGTTCAACCTTCTTCAACTTTTTCTTCCGAGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGATTTGACAGTGGATGTATTACGTTCAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTCGTGCCATTCTAAAGATACTTACAGCGAAGGACTATGCTCTTATACTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGCGAGGTGCATCAATTTTGCTCACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTGCGCATTGGAAGACACGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCTTTTCAGCAATGAACATACAAAGTGTGGAAGACATTAAGTCAAGATTGGACCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAACAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTTGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCCCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCGCATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGTATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGACAGATTGCGTTGTGGATCTCCTGAGATAGCCAATGCCAAACTCAGCTTCTCAAGTTTGAAGTCTCATTCATTGTGGATAAAGGAGGTTTTAAATCCAAGCAAACCAGTTATATTTATTAATACAGATTCTTTACCTGCTTTTGAGGCAAAGGACCACAAGATTGTGAATAACCCGATGGAAGCTCACATAATTGTAGAGGTCGTGGAGGAATTAGTTAACAGTGGAATTGAAGGGGATGATATCGGCATCATTACCCCGTATAACTCCCAGGCAGAGATCATTCGGCTTGCCATCAACTTTACTTCCGTGGAGATACATACCATTGACAAATACCAGGGAAGAGATAAAGATTGCATACTAGTCTCCTTTGTTAGGTCAACTGAAAATCCACAAAACTGCTCGTCCTCTCTGCTCGGAGACTGGCATAGGATTAATGTGGCTCTAACTCGTGCCAAGAAAAAGTTGATAATGGTCGGGTCACGCGAAACCCTATCCAAAGTTCTGCTGATGAAGCTTCTTATCGAGAAAGTTGATGAACAATCAGGCATCTTAAACGTGTCCAAGAAAGACATCAACTTTAAGAGGGAGCTCAAGAGATGTTCTCAGGTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1368

Amino Acids

151.97

Weight (kDa)

7.56

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 384 - 586 1.5e-62 DNA replication factor Dna2
AAA_11 PF13086 947 - 1038 1.8e-16 AAA domain
AAA_30 PF13604 948 - 1111 2.5e-09 AAA domain
AAA_19 PF13245 952 - 1111 4.5e-07 AAA domain
AAA_11 PF13086 1047 - 1115 5.9e-16 AAA domain
AAA_12 PF13087 1124 - 1324 9.2e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
AatII GACGTC 1 cut(s) 518
Acc16I TGCGCA 1 cut(s) 3059
Acc36I ACCTGC 2 cut(s) 328, 3631
Acc65I GGTACC 1 cut(s) 1887
AccB1I GGYRCC 1 cut(s) 1887
AccB7I CCANNNNNTGG 1 cut(s) 1721
AccI GTMKAC 2 cut(s) 1093, 1551
AccII CGCG 1 cut(s) 3972
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 9 cut(s) 48, 121, 207, 218, 365, 881, 2139, 2269, 2650
AclI AACGTT 2 cut(s) 1230, 1559
AclWI GGATC 9 cut(s) 232, 726, 738, 1278, 1654, 2801, 2814, 3197, 3511
AcoI YGGCCR 1 cut(s) 2270
AcsI RAATTY 9 cut(s) 355, 1241, 1640, 2366, 2459, 2666, 2839, 3001, 3218
AcuI CTGAAG 2 cut(s) 1644, 2601
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 6 cut(s) 334, 948, 1889, 2389, 3346, 3437
AflIII ACRYGT 1 cut(s) 4044
AhdI GACNNNNNGTC 2 cut(s) 2033, 3142
AhlI ACTAGT 3 cut(s) 274, 3194, 3847
AjiI CACGTC 1 cut(s) 507
AjuI GAANNNNNNNTTGG 4 cut(s) 1046, 1078, 3518, 3550
Alw21I GWGCWC 4 cut(s) 274, 1117, 2099, 4080
Alw26I GTCTC 6 cut(s) 60, 412, 522, 3555, 3856, 3900
Alw44I GTGCAC 2 cut(s) 270, 2095
AlwI GGATC 9 cut(s) 232, 726, 738, 1278, 1654, 2801, 2814, 3197, 3511
AlwNI CAGNNNCTG 1 cut(s) 950
Ama87I CYCGRG 4 cut(s) 293, 1041, 1323, 3354
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 5 cut(s) 878, 2019, 2270, 3275, 3351
ApaLI GTGCAC 2 cut(s) 270, 2095
ApeKI GCWGC 4 cut(s) 1331, 1439, 2136, 2441
ApoI RAATTY 9 cut(s) 355, 1241, 1640, 2366, 2459, 2666, 2839, 3001, 3218
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AseI ATTAAT 2 cut(s) 3606, 3921
Asp700I GAANNNNTTC 2 cut(s) 1601, 3090
Asp718I GGTACC 1 cut(s) 1887
AspA2I CCTAGG 1 cut(s) 1349
AspLEI GCGC 2 cut(s) 3060, 3403
AspS9I GGNCC 6 cut(s) 1834, 2829, 3154, 3275, 3352, 3640
AsuC2I CCSGG 1 cut(s) 1819
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 4 cut(s) 293, 1041, 1323, 3354
AvaII GGWCC 4 cut(s) 1834, 2829, 3154, 3640
AvrII CCTAGG 1 cut(s) 1349
AxyI CCTNAGG 2 cut(s) 2201, 3408
BaeGI GKGCMC 4 cut(s) 274, 1852, 2099, 2728
BamHI GGATCC 1 cut(s) 2806
BanI GGYRCC 1 cut(s) 1887
BanII GRGCYC 3 cut(s) 1500, 1714, 4080
BarI GAAGNNNNNNTAC 2 cut(s) 930, 962
BauI CACGAG 1 cut(s) 3936
BbsI GAAGAC 5 cut(s) 1594, 1904, 2391, 3073, 3141
Bbv12I GWGCWC 4 cut(s) 274, 1117, 2099, 4080
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 4 cut(s) 1343, 1426, 2148, 2428
BccI CCATC 8 cut(s) 482, 845, 1696, 2091, 2108, 2236, 3658, 3788
BceAI ACGGC 1 cut(s) 658
BcgI CGANNNNNNTGC 2 cut(s) 3084, 3118
BclI TGATCA 3 cut(s) 1933, 2851, 3313
BcnI CCSGG 1 cut(s) 1819
BcoDI GTCTC 6 cut(s) 60, 412, 522, 3555, 3856, 3900
BcuI ACTAGT 3 cut(s) 274, 3194, 3847
BfaI CTAG 8 cut(s) 9, 275, 1350, 2762, 2772, 2906, 3195, 3848
BfuAI ACCTGC 2 cut(s) 328, 3631
BglII AGATCT 1 cut(s) 1961
BisI GCNGC 6 cut(s) 881, 1332, 1440, 2137, 2270, 2442
BlnI CCTAGG 1 cut(s) 1349
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 6 cut(s) 882, 1333, 1441, 2138, 2271, 2443
Bme18I GGWCC 4 cut(s) 1834, 2829, 3154, 3640
BmeRI GACNNNNNGTC 2 cut(s) 2033, 3142
BmeT110I CYCGRG 4 cut(s) 293, 1041, 1323, 3354
BmgBI CACGTC 1 cut(s) 507
BmgT120I GGNCC 6 cut(s) 1834, 2829, 3154, 3275, 3352, 3640
BmiI GGNNCC 7 cut(s) 6, 1499, 1713, 1889, 2769, 2808, 2831
BpiI GAAGAC 5 cut(s) 1594, 1904, 2391, 3073, 3141
BplI GAGNNNNNCTC 5 cut(s) 392, 424, 502, 534, 4075
BpmI CTGGAG 4 cut(s) 1236, 1383, 1484, 1910
Bpu10I CCTNAGC 2 cut(s) 84, 180
Bpu1102I GCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 3 cut(s) 2008, 3523, 4064
BpuMI CCSGG 1 cut(s) 1819
BsaBI GATNNNNATC 2 cut(s) 729, 3318
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 9 cut(s) 457, 869, 1252, 1349, 1614, 2600, 3756, 3795, 3823
BsaWI WCCGGW 4 cut(s) 232, 412, 520, 715
BsaXI ACNNNNNCTCC 2 cut(s) 1209, 1239
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 8 cut(s) 1912, 1927, 2219, 2395, 3263, 3433, 3593, 3914
Bse21I CCTNAGG 2 cut(s) 2201, 3408
Bse3DI GCAATG 6 cut(s) 893, 1206, 1390, 3100, 3120, 3422
Bse8I GATNNNNATC 2 cut(s) 729, 3318
BseAI TCCGGA 1 cut(s) 232
BseDI CCNNGG 9 cut(s) 457, 869, 1252, 1349, 1614, 2600, 3756, 3795, 3823
BseJI GATNNNNATC 2 cut(s) 729, 3318
BseMI GCAATG 6 cut(s) 893, 1206, 1390, 3100, 3120, 3422
BseNI ACTGG 8 cut(s) 1912, 1927, 2219, 2395, 3263, 3433, 3593, 3914
BseRI GAGGAG 4 cut(s) 159, 416, 1712, 3321
BseSI GKGCMC 4 cut(s) 274, 1852, 2099, 2728
BseXI GCAGC 4 cut(s) 1343, 1426, 2148, 2428
BsgI GTGCAG 2 cut(s) 1425, 2089
Bsh1236I CGCG 1 cut(s) 3972
BshFI GGCC 5 cut(s) 880, 2021, 2272, 3277, 3353
BshNI GGYRCC 1 cut(s) 1887
BsiHKAI GWGCWC 4 cut(s) 274, 1117, 2099, 4080
BsiHKCI CYCGRG 4 cut(s) 293, 1041, 1323, 3354
BsiSI CCGG 7 cut(s) 233, 301, 413, 521, 651, 716, 1818
BslFI GGGAC 5 cut(s) 667, 1559, 2225, 2815, 3183
BsmAI GTCTC 6 cut(s) 60, 412, 522, 3555, 3856, 3900
BsmBI CGTCTC 2 cut(s) 60, 522
BsmFI GGGAC 5 cut(s) 667, 1559, 2225, 2815, 3183
BsmI GAATGC 2 cut(s) 2443, 2916
BsnI GGCC 5 cut(s) 880, 2021, 2272, 3277, 3353
Bso31I GGTCTC 1 cut(s) 412
BsoBI CYCGRG 4 cut(s) 293, 1041, 1323, 3354
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 8 cut(s) 274, 1117, 1500, 1714, 1852, 2099, 2728, 4080
Bsp13I TCCGGA 1 cut(s) 232
Bsp1720I GCTNAGC 1 cut(s) 78
BspACI CCGC 9 cut(s) 48, 121, 207, 218, 365, 881, 2139, 2269, 2650
BspANI GGCC 5 cut(s) 880, 2021, 2272, 3277, 3353
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 3972
BspHI TCATGA 2 cut(s) 820, 1537
BspLI GGNNCC 7 cut(s) 6, 1499, 1713, 1889, 2769, 2808, 2831
BspMI ACCTGC 2 cut(s) 328, 3631
BspPI GGATC 9 cut(s) 232, 726, 738, 1278, 1654, 2801, 2814, 3197, 3511
BspQI GCTCTTC 1 cut(s) 2279
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 1887
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 6 cut(s) 893, 1206, 1390, 3100, 3120, 3422
BsrFI RCCGGY 1 cut(s) 300
BsrI ACTGG 8 cut(s) 1912, 1927, 2219, 2395, 3263, 3433, 3593, 3914
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 9 cut(s) 457, 869, 1252, 1349, 1614, 2600, 3756, 3795, 3823
BssNAI GTATAC 1 cut(s) 1552
BssNI GRCGYC 1 cut(s) 515
BssSI CACGAG 1 cut(s) 3936
BssT1I CCWWGG 2 cut(s) 1252, 1349
Bst1107I GTATAC 1 cut(s) 1552
Bst2BI CACGAG 1 cut(s) 3936
Bst6I CTCTTC 8 cut(s) 166, 205, 1158, 1182, 1875, 2279, 3335, 3823
BstACI GRCGYC 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 269
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 6 cut(s) 623, 831, 2946, 3207, 3389, 3777
BstDSI CCRYGG 1 cut(s) 3795
BstEII GGTNACC 1 cut(s) 2486
BstENI CCTNNNNNAGG 2 cut(s) 2919, 3859
BstFNI CGCG 1 cut(s) 3972
BstHHI GCGC 2 cut(s) 3060, 3403
BstMAI GTCTC 6 cut(s) 60, 412, 522, 3555, 3856, 3900
BstNSI RCATGY 7 cut(s) 1001, 1126, 1487, 1530, 1901, 2948, 3183
BstPI GGTNACC 1 cut(s) 2486
BstSLI GKGCMC 4 cut(s) 274, 1852, 2099, 2728
BstUI CGCG 1 cut(s) 3972
BstV1I GCAGC 4 cut(s) 1343, 1426, 2148, 2428
BstV2I GAAGAC 5 cut(s) 1594, 1904, 2391, 3073, 3141
BstX2I RGATCY 5 cut(s) 237, 1646, 1961, 2806, 3503
BstYI RGATCY 5 cut(s) 237, 1646, 1961, 2806, 3503
BstZ17I GTATAC 1 cut(s) 1552
Bsu36I CCTNAGG 2 cut(s) 2201, 3408
BsuRI GGCC 5 cut(s) 880, 2021, 2272, 3277, 3353
BtgI CCRYGG 1 cut(s) 3795
BtrI CACGTC 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 2512
BtsIMutI CAGTG 6 cut(s) 1124, 1207, 1265, 2512, 2788, 3715
BveI ACCTGC 2 cut(s) 328, 3631
Cac8I GCNNGC 6 cut(s) 623, 831, 2946, 3207, 3389, 3777
CaiI CAGNNNCTG 1 cut(s) 950
CciI TCATGA 2 cut(s) 820, 1537
CfoI GCGC 2 cut(s) 3060, 3403
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 6 cut(s) 1834, 2829, 3154, 3275, 3352, 3640
CseI GACGC 1 cut(s) 2846
Csp6I GTAC 6 cut(s) 333, 947, 1888, 2388, 3345, 3436
CspCI CAANNNNNGTGG 4 cut(s) 193, 228, 2262, 2297
CviQI GTAC 6 cut(s) 333, 947, 1888, 2388, 3345, 3436
DraI TTTAAA 3 cut(s) 1813, 2844, 3579
DriI GACNNNNNGTC 2 cut(s) 2033, 3142
EaeI YGGCCR 1 cut(s) 2270
Eam1104I CTCTTC 8 cut(s) 166, 205, 1158, 1182, 1875, 2279, 3335, 3823
Eam1105I GACNNNNNGTC 2 cut(s) 2033, 3142
EarI CTCTTC 8 cut(s) 166, 205, 1158, 1182, 1875, 2279, 3335, 3823
Ecl136II GAGCTC 1 cut(s) 4078
Eco130I CCWWGG 2 cut(s) 1252, 1349
Eco147I AGGCCT 1 cut(s) 2021
Eco24I GRGCYC 3 cut(s) 1500, 1714, 4080
Eco31I GGTCTC 1 cut(s) 412
Eco32I GATATC 1 cut(s) 3730
Eco47I GGWCC 4 cut(s) 1834, 2829, 3154, 3640
Eco53kI GAGCTC 1 cut(s) 4078
Eco57I CTGAAG 2 cut(s) 1644, 2601
Eco81I CCTNAGG 2 cut(s) 2201, 3408
Eco88I CYCGRG 4 cut(s) 293, 1041, 1323, 3354
Eco91I GGTNACC 1 cut(s) 2486
EcoICRI GAGCTC 1 cut(s) 4078
EcoNI CCTNNNNNAGG 2 cut(s) 2919, 3859
EcoO65I GGTNACC 1 cut(s) 2486
EcoRV GATATC 1 cut(s) 3730
EcoT14I CCWWGG 2 cut(s) 1252, 1349
EcoT22I ATGCAT 2 cut(s) 1528, 3233
EcoT38I GRGCYC 3 cut(s) 1500, 1714, 4080
ErhI CCWWGG 2 cut(s) 1252, 1349
Esp3I CGTCTC 2 cut(s) 60, 522
FaqI GGGAC 5 cut(s) 667, 1559, 2225, 2815, 3183
FauI CCCGC 1 cut(s) 2643
FbaI TGATCA 3 cut(s) 1933, 2851, 3313
FblI GTMKAC 2 cut(s) 1093, 1551
Fnu4HI GCNGC 6 cut(s) 881, 1332, 1440, 2137, 2270, 2442
FriOI GRGCYC 3 cut(s) 1500, 1714, 4080
Fsp4HI GCNGC 6 cut(s) 881, 1332, 1440, 2137, 2270, 2442
FspAI RTGCGCAY 1 cut(s) 3059
FspBI CTAG 8 cut(s) 9, 275, 1350, 2762, 2772, 2906, 3195, 3848
FspI TGCGCA 1 cut(s) 3059
GlaI GCGC 2 cut(s) 3059, 3402
GluI GCNGC 6 cut(s) 881, 1332, 1440, 2137, 2270, 2442
GsuI CTGGAG 4 cut(s) 1236, 1383, 1484, 1910
HaeIII GGCC 5 cut(s) 880, 2021, 2272, 3277, 3353
HapII CCGG 7 cut(s) 233, 301, 413, 521, 651, 716, 1818
HgaI GACGC 1 cut(s) 2846
HhaI GCGC 2 cut(s) 3060, 3403
Hin1I GRCGYC 1 cut(s) 515
Hin6I GCGC 2 cut(s) 3058, 3401
HinP1I GCGC 2 cut(s) 3058, 3401
HincII GTYRAC 5 cut(s) 1228, 2656, 2758, 3706, 3867
HindII GTYRAC 5 cut(s) 1228, 2656, 2758, 3706, 3867
HindIII AAGCTT 5 cut(s) 827, 1626, 2062, 3376, 4001
HpaI GTTAAC 1 cut(s) 3706
HpaII CCGG 7 cut(s) 233, 301, 413, 521, 651, 716, 1818
Hpy99I CGWCG 2 cut(s) 2640, 2862
HpyCH4IV ACGT 8 cut(s) 506, 515, 1230, 1559, 1599, 1990, 2795, 4044
HpySE526I ACGT 8 cut(s) 506, 515, 1230, 1559, 1599, 1990, 2795, 4044
Hsp92I GRCGYC 1 cut(s) 515
HspAI GCGC 2 cut(s) 3058, 3401
Kpn2I TCCGGA 1 cut(s) 232
KpnI GGTACC 1 cut(s) 1891
Ksp22I TGATCA 3 cut(s) 1933, 2851, 3313
KspAI GTTAAC 1 cut(s) 3706
LguI GCTCTTC 1 cut(s) 2279
LmnI GCTCC 7 cut(s) 10, 1027, 1503, 1717, 1840, 2773, 4075
Lsp1109I GCAGC 4 cut(s) 1343, 1426, 2148, 2428
MaeI CTAG 8 cut(s) 9, 275, 1350, 2762, 2772, 2906, 3195, 3848
MaeII ACGT 8 cut(s) 506, 515, 1230, 1559, 1599, 1990, 2795, 4044
MflI RGATCY 5 cut(s) 237, 1646, 1961, 2806, 3503
MhlI GDGCHC 8 cut(s) 274, 1117, 1500, 1714, 1852, 2099, 2728, 4080
MlyI GAGTC 4 cut(s) 191, 487, 793, 2065
MmeI TCCRAC 3 cut(s) 130, 1049, 1860
Mph1103I ATGCAT 2 cut(s) 1528, 3233
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 2 cut(s) 1601, 3090
MslI CAYNNNNRTG 5 cut(s) 1302, 1542, 2088, 2479, 2562
MspA1I CMGCKG 1 cut(s) 2139
MspI CCGG 7 cut(s) 233, 301, 413, 521, 651, 716, 1818
Mva1269I GAATGC 2 cut(s) 2443, 2916
MvnI CGCG 1 cut(s) 3972
NciI CCSGG 1 cut(s) 1819
NlaIV GGNNCC 7 cut(s) 6, 1499, 1713, 1889, 2769, 2808, 2831
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 8 cut(s) 340, 502, 1015, 1669, 2486, 2555, 3485, 3966
NsbI TGCGCA 1 cut(s) 3059
NsiI ATGCAT 2 cut(s) 1528, 3233
NspI RCATGY 7 cut(s) 1001, 1126, 1487, 1530, 1901, 2948, 3183
NspV TTCGAA 1 cut(s) 422
PaeI GCATGC 1 cut(s) 2948
PagI TCATGA 2 cut(s) 820, 1537
PaqCI CACCTGC 1 cut(s) 328
PceI AGGCCT 1 cut(s) 2021
PciSI GCTCTTC 1 cut(s) 2279
PcsI WCGNNNNNNNCGW 2 cut(s) 512, 3969
PctI GAATGC 2 cut(s) 2443, 2916
PdmI GAANNNNTTC 2 cut(s) 1601, 3090
PflFI GACNNNGTC 2 cut(s) 1260, 3158
PflMI CCANNNNNTGG 1 cut(s) 1721
PkrI GCNGC 6 cut(s) 882, 1333, 1441, 2138, 2271, 2443
PleI GAGTC 4 cut(s) 191, 486, 792, 2064
PpsI GAGTC 4 cut(s) 191, 486, 792, 2064
PshBI ATTAAT 2 cut(s) 3606, 3921
Psp124BI GAGCTC 1 cut(s) 4080
Psp1406I AACGTT 2 cut(s) 1230, 1559
PspEI GGTNACC 1 cut(s) 2486
PspN4I GGNNCC 7 cut(s) 6, 1499, 1713, 1889, 2769, 2808, 2831
PspPI GGNCC 6 cut(s) 1834, 2829, 3154, 3275, 3352, 3640
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 5 cut(s) 237, 1646, 1961, 2806, 3503
PsyI GACNNNGTC 2 cut(s) 1260, 3158
RsaI GTAC 6 cut(s) 334, 948, 1889, 2389, 3346, 3437
RsaNI GTAC 6 cut(s) 333, 947, 1888, 2388, 3345, 3436
RseI CAYNNNNRTG 5 cut(s) 1302, 1542, 2088, 2479, 2562
SacI GAGCTC 1 cut(s) 4080
SapI GCTCTTC 1 cut(s) 2279
SatI GCNGC 6 cut(s) 881, 1332, 1440, 2137, 2270, 2442
Sau96I GGNCC 6 cut(s) 1834, 2829, 3154, 3275, 3352, 3640
SchI GAGTC 4 cut(s) 191, 487, 793, 2065
SduI GDGCHC 8 cut(s) 274, 1117, 1500, 1714, 1852, 2099, 2728, 4080
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 4 cut(s) 1834, 2829, 3154, 3640
SmiI ATTTAAAT 1 cut(s) 2844
SmiMI CAYNNNNRTG 5 cut(s) 1302, 1542, 2088, 2479, 2562
SmlI CTYRAG 3 cut(s) 2023, 3538, 4079
SmoI CTYRAG 3 cut(s) 2023, 3538, 4079
SpeI ACTAGT 3 cut(s) 274, 3194, 3847
SphI GCATGC 1 cut(s) 2948
SseBI AGGCCT 1 cut(s) 2021
SsiI CCGC 9 cut(s) 48, 121, 207, 218, 365, 881, 2139, 2269, 2650
SspI AATATT 2 cut(s) 1584, 3297
SspMI CTAG 8 cut(s) 9, 275, 1350, 2762, 2772, 2906, 3195, 3848
SstI GAGCTC 1 cut(s) 4080
StuI AGGCCT 1 cut(s) 2021
StyI CCWWGG 2 cut(s) 1252, 1349
SwaI ATTTAAAT 1 cut(s) 2844
TaiI ACGT 8 cut(s) 509, 518, 1233, 1562, 1602, 1993, 2798, 4047
TaqI TCGA 9 cut(s) 65, 71, 227, 317, 422, 750, 1764, 3094, 4011
TatI WGTACW 1 cut(s) 3344
TauI GCSGC 2 cut(s) 883, 2272
TscAI CASTG 6 cut(s) 1124, 1207, 1265, 2512, 2788, 3715
TseFI GTSAC 8 cut(s) 340, 502, 1015, 1669, 2486, 2555, 3485, 3966
TseI GCWGC 4 cut(s) 1331, 1439, 2136, 2441
Tsp45I GTSAC 8 cut(s) 340, 502, 1015, 1669, 2486, 2555, 3485, 3966
TspGWI ACGGA 2 cut(s) 2068, 3784
TspRI CASTG 6 cut(s) 1124, 1207, 1265, 2512, 2788, 3715
Tth111I GACNNNGTC 2 cut(s) 1260, 3158
Van91I CCANNNNNTGG 1 cut(s) 1721
VneI GTGCAC 2 cut(s) 270, 2095
VpaK11BI GGWCC 4 cut(s) 1834, 2829, 3154, 3640
VspI ATTAAT 2 cut(s) 3606, 3921
XagI CCTNNNNNAGG 2 cut(s) 2919, 3859
XapI RAATTY 9 cut(s) 355, 1241, 1640, 2366, 2459, 2666, 2839, 3001, 3218
XceI RCATGY 7 cut(s) 1001, 1126, 1487, 1530, 1901, 2948, 3183
XmaJI CCTAGG 1 cut(s) 1349
XmiI GTMKAC 2 cut(s) 1093, 1551
XmnI GAANNNNTTC 2 cut(s) 1601, 3090
XspI CTAG 8 cut(s) 9, 275, 1350, 2762, 2772, 2906, 3195, 3848
ZraI GACGTC 1 cut(s) 516
Zsp2I ATGCAT 2 cut(s) 1528, 3233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.