MD02G1227500.v1.1

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002244
Physical Location & Seq
Forward (+)
26953302 .. 26958808
5507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1227500.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGACTATCCCAGGAATAGCTAGTAGGCAGAGTCCTTTCCGAACTCCACCTTCTCTATTGTACTTCCATGATAAGCTTGCTAATGGTGTTGCATGTAATGGAGTTTCAGATCAGATGGGGTTGAGGCAACATAAAAAGGCATTGCTTGAACTCTTAGATAAAGTGGAAGATGTTATTTCTATTGAAGATTCAGTGTCTAGAGATGTGGAGGAATCTCCATTTCAAGTTGAAGATAAAAAAGGCAAAAGAATGCCTGTCAGTTTGGATAACACAGTGAAAAGAGCAGAGACAGCTCTAACAGAAACGGTTACTCGGAAATCTTCCTATTGTAGTTTTCTTGTATTGGAGGTATCTGAGAAGAGTGGAGATGCTGATTCATCATGTGATCAATGCCTGTTTAAGGTTATTTGGTTACGAAATGAGCAAAGTGGAGAAGAGAGCTCTGTATATTTACTAGATGAGTGGTTTTACAGTGTCATTGCTCCTGGAGATACTGTCAATGTTATTGGTGAATTTGATAACCAAGGACAGTGCCAAGTGGATCGTCACAATAATTTTATAATTGTTCATCCGGATGTTTTGGTGTCCGGAACTAGGGTTACAGCTAGCTTCGTTTGCCCGAGGCGAACTGTTCTGGATGAAAGACTAAAATCCAATGAGCATTCAACTGCAGCACTAAGTGGTACAGCTGGACTTATGAAGGAGATACCTACACTAACTTTTTTAGAGGAACGCACAAGAGTGGTGCTCCAGAAACACCTTGAGAACTTGTATGCTTGTGGAGGGAACAAAAATGATATGTACAAAACATTGATTGAGGCTGTACCCAAAATATTGAATTGGGTTAACCTCTTCAAAAATTCTCAGGATTCAAAAGCTCCTTCTGTTAATTTTGGATCTGACAATGGGGTGAAAAAAGTAATTGATATTGAGGAGATGGCCTGGGCTCCAAAATATGGTTTGAAAGGCCAGATTGATGCTTCTGTCAGAGTGAAAGTTGAATCCAGCAATGGTGAATCTCATGGGAAAGTTATGCCTTTAGAGTTCAAAAGTGGGAAAGTACCCAAGGGCCAGTCATCTATGGAGCATAGTGCCCAAGTGATCTTATACACTCTCCTCATGTCGGACAGCATGTCGATACTGGTCTCCTATGTTATCTGCAATCAGATCAGACACAGGGAATTATGGCTAGAAGATCTGACCTGGTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

405

Amino Acids

45.15

Weight (kDa)

5.7

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 141 - 331 8.5e-51 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB7I CCANNNNNTGG 1 cut(s) 956
AccIII TCCGGA 2 cut(s) 571, 587
AclWI GGATC 2 cut(s) 549, 904
AcsI RAATTY 2 cut(s) 512, 859
AdeI CACNNNGTG 1 cut(s) 680
AfaI GTAC 5 cut(s) 62, 685, 803, 825, 1062
AfiI CCNNNNNNNGG 4 cut(s) 400, 594, 956, 1123
AhdI GACNNNNNGTC 1 cut(s) 1132
AjnI CCWGG 4 cut(s) 10, 484, 941, 1202
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 8 cut(s) 20, 76, 293, 441, 605, 609, 689, 878
AluI AGCT 8 cut(s) 20, 76, 293, 441, 605, 609, 689, 878
Alw21I GWGCWC 2 cut(s) 443, 750
Alw26I GTCTC 2 cut(s) 281, 1150
AlwI GGATC 2 cut(s) 549, 904
Ama87I CYCGRG 1 cut(s) 619
Aor13HI TCCGGA 2 cut(s) 571, 587
AoxI GGCC 3 cut(s) 939, 967, 1069
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 2 cut(s) 512, 859
AspS9I GGNCC 1 cut(s) 1069
AsuHPI GGTGA 3 cut(s) 521, 922, 1025
AsuNHI GCTAGC 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 619
BaeGI GKGCMC 1 cut(s) 1096
BanII GRGCYC 2 cut(s) 443, 949
Bbv12I GWGCWC 2 cut(s) 443, 750
BbvI GCAGC 1 cut(s) 683
BccI CCATC 2 cut(s) 109, 931
BciT130I CCWGG 4 cut(s) 12, 486, 943, 1204
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 281, 1150
BfaI CTAG 6 cut(s) 21, 198, 455, 594, 606, 1190
BfmI CTRYAG 1 cut(s) 669
BglII AGATCT 1 cut(s) 1195
BisI GCNGC 1 cut(s) 672
BlsI GCNGC 1 cut(s) 673
Bme1390I CCNGG 4 cut(s) 12, 486, 943, 1204
BmeRI GACNNNNNGTC 1 cut(s) 1132
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 1 cut(s) 948
BmrFI CCNGG 4 cut(s) 12, 486, 943, 1204
BmsI GCATC 2 cut(s) 358, 967
BmtI GCTAGC 1 cut(s) 609
BplI GAGNNNNNCTC 2 cut(s) 732, 764
BpmI CTGGAG 2 cut(s) 507, 734
BpuEI CTTGAG 1 cut(s) 782
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 5 cut(s) 10, 523, 620, 942, 1065
BsaWI WCCGGW 2 cut(s) 571, 587
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 4 cut(s) 400, 594, 956, 1123
Bse1I ACTGG 2 cut(s) 1072, 1146
Bse3DI GCAATG 3 cut(s) 140, 477, 1015
BseAI TCCGGA 2 cut(s) 571, 587
BseBI CCWGG 4 cut(s) 12, 486, 943, 1204
BseDI CCNNGG 5 cut(s) 10, 523, 620, 942, 1065
BseGI GGATG 3 cut(s) 568, 580, 643
BseLI CCNNNNNNNGG 4 cut(s) 400, 594, 956, 1123
BseMI GCAATG 3 cut(s) 140, 477, 1015
BseMII CTCAG 2 cut(s) 345, 878
BseNI ACTGG 2 cut(s) 1072, 1146
BseRI GAGGAG 2 cut(s) 947, 1106
BseSI GKGCMC 1 cut(s) 1096
BseXI GCAGC 1 cut(s) 683
BshFI GGCC 3 cut(s) 941, 969, 1071
BsiHKAI GWGCWC 2 cut(s) 443, 750
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 2 cut(s) 572, 588
BslI CCNNNNNNNGG 4 cut(s) 400, 594, 956, 1123
BsmAI GTCTC 2 cut(s) 281, 1150
BsmI GAATGC 2 cut(s) 255, 661
BsnI GGCC 3 cut(s) 941, 969, 1071
Bso31I GGTCTC 1 cut(s) 1150
BsoBI CYCGRG 1 cut(s) 619
Bsp1286I GDGCHC 4 cut(s) 443, 750, 949, 1096
Bsp13I TCCGGA 2 cut(s) 571, 587
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
BspANI GGCC 3 cut(s) 941, 969, 1071
BspCNI CTCAG 2 cut(s) 346, 877
BspEI TCCGGA 2 cut(s) 571, 587
BspLI GGNNCC 1 cut(s) 948
BspMAI CTGCAG 1 cut(s) 673
BspOI GCTAGC 1 cut(s) 609
BspPI GGATC 2 cut(s) 549, 904
BspTNI GGTCTC 1 cut(s) 1150
BsrDI GCAATG 3 cut(s) 140, 477, 1015
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 2 cut(s) 1072, 1146
BssECI CCNNGG 5 cut(s) 10, 523, 620, 942, 1065
BssMI GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
BssT1I CCWWGG 2 cut(s) 523, 1065
Bst2UI CCWGG 4 cut(s) 12, 486, 943, 1204
Bst4CI ACNGT 6 cut(s) 274, 307, 473, 496, 531, 631
Bst6I CTCTTC 3 cut(s) 353, 429, 857
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 78, 607
BstDEI CTNAG 4 cut(s) 154, 354, 677, 864
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 3 cut(s) 568, 580, 643
BstKTI GATC 7 cut(s) 112, 388, 544, 899, 1104, 1170, 1198
BstMAI GTCTC 2 cut(s) 281, 1150
BstMBI GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
BstMWI GCNNNNNNNGC 2 cut(s) 290, 615
BstNI CCWGG 4 cut(s) 12, 486, 943, 1204
BstNSI RCATGY 2 cut(s) 96, 1135
BstSCI CCNGG 4 cut(s) 10, 484, 941, 1202
BstSFI CTRYAG 1 cut(s) 669
BstSLI GKGCMC 1 cut(s) 1096
BstV1I GCAGC 1 cut(s) 683
BstX2I RGATCY 2 cut(s) 896, 1195
BstYI RGATCY 2 cut(s) 896, 1195
BsuRI GGCC 3 cut(s) 941, 969, 1071
BtsCI GGATG 3 cut(s) 568, 580, 643
BtsIMutI CAGTG 4 cut(s) 198, 279, 478, 536
Cac8I GCNNGC 2 cut(s) 78, 607
Cfr13I GGNCC 1 cut(s) 1069
CsiI ACCWGGT 1 cut(s) 1202
Csp6I GTAC 5 cut(s) 61, 684, 802, 824, 1061
CviAII CATG 6 cut(s) 68, 93, 381, 1022, 1120, 1132
CviQI GTAC 5 cut(s) 61, 684, 802, 824, 1061
DdeI CTNAG 4 cut(s) 154, 354, 677, 864
DpnI GATC 7 cut(s) 111, 387, 543, 898, 1103, 1169, 1197
DpnII GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
DraIII CACNNNGTG 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 1132
Eam1104I CTCTTC 3 cut(s) 353, 429, 857
Eam1105I GACNNNNNGTC 1 cut(s) 1132
EarI CTCTTC 3 cut(s) 353, 429, 857
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 2 cut(s) 523, 1065
Eco24I GRGCYC 2 cut(s) 443, 949
Eco31I GGTCTC 1 cut(s) 1150
Eco53kI GAGCTC 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 441
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 4 cut(s) 10, 484, 941, 1202
EcoT14I CCWWGG 2 cut(s) 523, 1065
EcoT38I GRGCYC 2 cut(s) 443, 949
ErhI CCWWGG 2 cut(s) 523, 1065
FaeI CATG 6 cut(s) 71, 96, 384, 1025, 1123, 1135
FatI CATG 6 cut(s) 67, 92, 380, 1021, 1119, 1131
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 672
FokI GGATG 3 cut(s) 555, 587, 650
FriOI GRGCYC 2 cut(s) 443, 949
Fsp4HI GCNGC 1 cut(s) 672
FspBI CTAG 6 cut(s) 21, 198, 455, 594, 606, 1190
GluI GCNGC 1 cut(s) 672
GsuI CTGGAG 2 cut(s) 507, 734
HaeIII GGCC 3 cut(s) 941, 969, 1071
HapII CCGG 2 cut(s) 572, 588
Hin1II CATG 6 cut(s) 71, 96, 384, 1025, 1123, 1135
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 7 cut(s) 31, 188, 212, 374, 869, 1001, 1016
HpaI GTTAAC 1 cut(s) 847
HpaII CCGG 2 cut(s) 572, 588
HphI GGTGA 3 cut(s) 521, 922, 1025
Hpy166II GTNNAC 1 cut(s) 847
Hpy188III TCNNGA 6 cut(s) 198, 572, 588, 635, 751, 866
Hpy8I GTNNAC 1 cut(s) 847
HpyAV CCTTC 3 cut(s) 60, 694, 891
HpyCH4III ACNGT 6 cut(s) 274, 307, 473, 496, 531, 631
HpyCH4V TGCA 3 cut(s) 92, 671, 1161
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 615
HpyF3I CTNAG 4 cut(s) 154, 354, 677, 864
Hsp92II CATG 6 cut(s) 71, 96, 384, 1025, 1123, 1135
Kpn2I TCCGGA 2 cut(s) 571, 587
Ksp22I TGATCA 1 cut(s) 385
KspAI GTTAAC 1 cut(s) 847
Kzo9I GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
LmnI GCTCC 5 cut(s) 487, 753, 883, 952, 1084
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 2 cut(s) 358, 967
MabI ACCWGGT 1 cut(s) 1202
MaeI CTAG 6 cut(s) 21, 198, 455, 594, 606, 1190
MaeIII GTNAC 4 cut(s) 307, 411, 545, 598
MalI GATC 7 cut(s) 111, 387, 543, 898, 1103, 1169, 1197
MboI GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
MboII GAAGA 8 cut(s) 179, 197, 242, 312, 370, 446, 844, 1205
MflI RGATCY 2 cut(s) 896, 1195
MhlI GDGCHC 4 cut(s) 443, 750, 949, 1096
MluCI AATT 8 cut(s) 512, 553, 561, 838, 859, 889, 921, 1181
MlyI GAGTC 1 cut(s) 40
MmeI TCCRAC 1 cut(s) 1104
MroI TCCGGA 2 cut(s) 571, 587
MseI TTAA 3 cut(s) 399, 846, 888
MslI CAYNNNNRTG 2 cut(s) 573, 740
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 2 cut(s) 572, 588
MspR9I CCNGG 4 cut(s) 12, 486, 943, 1204
Mva1269I GAATGC 2 cut(s) 255, 661
MvaI CCWGG 4 cut(s) 12, 486, 943, 1204
MwoI GCNNNNNNNGC 2 cut(s) 290, 615
NdeII GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
NheI GCTAGC 1 cut(s) 605
NlaIII CATG 6 cut(s) 71, 96, 384, 1025, 1123, 1135
NlaIV GGNNCC 1 cut(s) 948
NmuCI GTSAC 1 cut(s) 545
NspI RCATGY 2 cut(s) 96, 1135
OliI CACNNNNGTG 1 cut(s) 740
PctI GAATGC 2 cut(s) 255, 661
PfeI GAWTC 6 cut(s) 188, 212, 374, 869, 1001, 1016
PflMI CCANNNNNTGG 1 cut(s) 956
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 1 cut(s) 673
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 4 cut(s) 10, 484, 941, 1202
PspGI CCWGG 4 cut(s) 10, 484, 941, 1202
PspN4I GGNNCC 1 cut(s) 948
PspPI GGNCC 1 cut(s) 1069
PsrI GAACNNNNNNTAC 2 cut(s) 583, 615
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 2 cut(s) 896, 1195
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 5 cut(s) 62, 685, 803, 825, 1062
RsaNI GTAC 5 cut(s) 61, 684, 802, 824, 1061
RseI CAYNNNNRTG 2 cut(s) 573, 740
SacI GAGCTC 1 cut(s) 443
SaqAI TTAA 3 cut(s) 399, 846, 888
SatI GCNGC 1 cut(s) 672
Sau3AI GATC 7 cut(s) 109, 385, 541, 896, 1101, 1167, 1195
Sau96I GGNCC 1 cut(s) 1069
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 4 cut(s) 12, 486, 943, 1204
SduI GDGCHC 4 cut(s) 443, 750, 949, 1096
SexAI ACCWGGT 1 cut(s) 1202
SfaNI GCATC 2 cut(s) 358, 967
SfcI CTRYAG 1 cut(s) 669
SmiMI CAYNNNNRTG 2 cut(s) 573, 740
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 8 cut(s) 512, 553, 561, 838, 859, 889, 921, 1181
SspI AATATT 1 cut(s) 834
SspMI CTAG 6 cut(s) 21, 198, 455, 594, 606, 1190
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 4 cut(s) 10, 484, 941, 1202
StyI CCWWGG 2 cut(s) 523, 1065
TaaI ACNGT 6 cut(s) 274, 307, 473, 496, 531, 631
TaqI TCGA 1 cut(s) 1136
TasI AATT 8 cut(s) 512, 553, 561, 838, 859, 889, 921, 1181
TatI WGTACW 2 cut(s) 60, 801
TfiI GAWTC 6 cut(s) 188, 212, 374, 869, 1001, 1016
Tru1I TTAA 3 cut(s) 399, 846, 888
Tru9I TTAA 3 cut(s) 399, 846, 888
TscAI CASTG 4 cut(s) 198, 279, 478, 536
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 4 cut(s) 366, 557, 654, 713
TspRI CASTG 4 cut(s) 198, 279, 478, 536
Van91I CCANNNNNTGG 1 cut(s) 956
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 2 cut(s) 512, 859
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 2 cut(s) 96, 1135
XspI CTAG 6 cut(s) 21, 198, 455, 594, 606, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.