RchiOBHm_Chr1g0343241

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002244
Physical Location & Seq
Reverse (-)
35172260 .. 35184624
12365 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56978

Sequence Viewer

Length: 4146 bp
ATGGCTCCTAGAAAGAAACCCAATCCTTCTTCTTCTACTACCGCTTCGTCTCGGAAATCGAACTCGAAAGCTCAGCCTCAGCCTTCCAAGTTCAGCATCCAACATTTCTTTGAGCGCCACACCCAGAACTCTCAAAACCCCCAAACCACCACCTCTTCCACTCCTCACAACCCTAAGCCCCCAATCGCCGACTCCGCCACCGCCATTGTTCCGCAGAAGTCGATTCCGGAGATCCAAAACCCTGCCCCGATTGAAGCATCTAGTGCACTAGTAGCTTCTGGAAATCCCGAGAATCGGCGTGATTCGCAGTCGAGTAATAAGAACAGTACGCAGGAGACACCAACTGAGAATTTGGTGGCGGTGAGGTTTATGGATGCGGAGCCGAATGTGGATGAGGAGGTCTCACCGGAGGTTTCGAAGTCTAAGTCTGTCAAGCGATTCAAGTTCTCCCCTGGAATGTTGATACAGCAGAGTCAAGATGATGGAGGTGATGAGGTGACGTGGAGGACGTCTCCGGTTAATGAAAGACTTAAAGCGGTTTCAAAGCACAAGCCTGAGATGATGAGAGTGTTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGCAAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGAGAAATCCCTGTCGTCGGTGTCCCTGAATAGGGTTGGCGTGAAGAGGGTTAAACCAGAACAGGATGTGGAACATAATGGAAATGTGGTCGATAGGACTATTTCTGAAATAGCTGGTAGTCAGAGTCCATTCCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTCCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATTTTTGTTGAAGATTCAGTACCTGATGGTGTGGAGGCATCTCAATTACCAGTTAAAGATAATTCAGAGCATGCTGTAAAAAGAGTGGTGACAGCTCCAACAGAACTGGTTACTCAGGCATCTTCCAATTACAATTTCCTTGTATTGGAGGTATCTGAGAATTGTGGACCTCATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGCGTGCTGTGTATTTACGGGAAGAGTGGTTTTACAGTGTCATTGCACCTGGAGATACTGTCAACGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATCATCACCATAACTTTTTAATTGTTCACCCCGATGTCTTAGTATCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCCTAGGCGAACTGTGCTGGATGAAAGGCTAAAAGGCAATGAGTATTCAACTCCAGCACTAATTGGCACAATATTGCATCAAGTTTTTGAGGCTGCACTTATGAAGGAGAAACCCACAATTACTTTCTTGGAGGAAAATGCAAGAATGGGGCTCCAGAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTGAACATCTTCAAACACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAGGTCACAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTTGAAGCTAACAAAAATGAATCTGACGTGAAGGTTATGCCTTTGGAGTTTAAAACCGGAAAAGTGCCGAAGGACCAGGCAAGACTATTCTCTGTACCAAACTCTCTTAGGGGATATTATACTCGAGAGCATTGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAGAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACGGAGTCCAAGCTTGTGCAGAAGTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGGCACATGGAGGCAACACAGATAGCAGCGGATTGGGATATTTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGACTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGCTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGTGATCATTCCACAAGTTGCCTTTCTTCTATTGTTCTTGACAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTGCTGGTTTGTCTTCTCTCAATGTGAGGACATCCAATGGAGATTTTCAGAATGCTGCTTCTTCACCCTCAAAATATGCAGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTAGCTGTTGCAAGGGGGGTCATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGGGCAATCCTTCTTCAGAGGCAAGTGATCTTGATCGGGAGGTCTGGCGGGTTGACAAAGATGAATTTATGACATCATTTGCCACAATGAGGTTCAACCTTCTTCAACTTTTTCTTCCGGGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGGTTTGACAGTGGATGTATTACGTTCAGTCAGGATCCAGCAATATCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTCGTGCCATTCTAAAGATACTTACAGCGAAGGACTACGCTCTTATACTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGCGAGGTGCATCAATTTTGCTTACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTGCGCATTGGAAGACAGGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCTTTTCAGCAATGAACATACAAAGTGTGGAAGACATTAAGTCAAGATTGGACCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAACAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTCGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCCCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCGCATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGAATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGACAGATTGCGTTGTGGATCTCCTGAAATAGCCAATGCCAAACTCAACTTTTCAAGTTCGAAGTCTCATTCATTGTGGATAAAGGAGGTTTTAAATCCAAGCAAACCAGTTATATTTATTAATACAGATGCTTTACCTGCTTTCGAGGCAAAGGACCACAAGATTGTGAATAACCCGATGGAAGCTCACATAGTTGTAGAGGTCGTGGAGGAATTAGTTAACAGTGGAATTGATGGGGATGATATTGGCATCATTACCCCGTATAACTCCCAGGCAGAGATCATTCGGCTTGCCATCAACCTTACTTCGGTGGAGATACATACCATTGACAAATACCAGGGAAGAGATAAAGATTGCATACTAGTCTCCTTTGTTAGGTCAACTGCAAATCCACGAAACTGCTCGTCCTCTCTGCTCGGAGACTGGCATAGGATTAATGTGGCTCTAACTCGTGCCAAGAAAAAGTTGATAATGGTCGGGTCACGCGAAACCCTATCCAAAGTTCTGCTGATGAAGCTTCTTATCGAGAAAGTTGATGAACAATCAGGCATCTTCAACGTGTCCAAGAAAGACATTAACTTTAAGAGGGAGCTCAAGAGATGCTCTCAGGTCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1381

Amino Acids

153.3

Weight (kDa)

7.4

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 382 - 584 4.8e-64 DNA replication factor Dna2
AAA_11 PF13086 961 - 1052 1.6e-16 AAA domain
AAA_30 PF13604 962 - 1125 2.7e-09 AAA domain
AAA_19 PF13245 966 - 1125 5.3e-07 AAA domain
AAA_11 PF13086 1061 - 1129 6e-16 AAA domain
AAA_12 PF13087 1138 - 1338 1.2e-53 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
Acc16I TGCGCA 1 cut(s) 3101
Acc36I ACCTGC 1 cut(s) 3673
Acc65I GGTACC 1 cut(s) 1929
AccB1I GGYRCC 2 cut(s) 1929, 2765
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccI GTMKAC 1 cut(s) 1545
AccII CGCG 1 cut(s) 4014
AccIII TCCGGA 1 cut(s) 226
AclI AACGTT 2 cut(s) 1224, 1553
AclWI GGATC 7 cut(s) 226, 1272, 1648, 2843, 2856, 3239, 3553
AcoI YGGCCR 1 cut(s) 2312
AcsI RAATTY 7 cut(s) 349, 1235, 2408, 2708, 2881, 3043, 3260
AcuI CTGAAG 2 cut(s) 1638, 2643
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 6 cut(s) 328, 942, 1851, 1931, 3388, 3479
AflIII ACRYGT 1 cut(s) 4086
AhdI GACNNNNNGTC 2 cut(s) 2075, 3184
AhlI ACTAGT 3 cut(s) 268, 3236, 3889
AjiI CACGTC 2 cut(s) 501, 1783
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
Alw21I GWGCWC 4 cut(s) 268, 1111, 2141, 4122
Alw26I GTCTC 7 cut(s) 54, 329, 406, 516, 3597, 3898, 3942
Alw44I GTGCAC 2 cut(s) 264, 2137
AlwI GGATC 7 cut(s) 226, 1272, 1648, 2843, 2856, 3239, 3553
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 5 cut(s) 872, 2061, 2312, 3317, 3393
ApaLI GTGCAC 2 cut(s) 264, 2137
ApeKI GCWGC 4 cut(s) 1325, 1433, 2178, 2483
ApoI RAATTY 7 cut(s) 349, 1235, 2408, 2708, 2881, 3043, 3260
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AseI ATTAAT 2 cut(s) 3648, 3963
Asp700I GAANNNNTTC 2 cut(s) 1595, 3132
Asp718I GGTACC 1 cut(s) 1929
AspA2I CCTAGG 2 cut(s) 1343, 2813
AspLEI GCGC 3 cut(s) 117, 3102, 3445
AspS9I GGNCC 7 cut(s) 1088, 1828, 2871, 3196, 3317, 3394, 3682
AsuC2I CCSGG 1 cut(s) 2763
AsuII TTCGAA 2 cut(s) 416, 3587
AvaI CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
AvaII GGWCC 5 cut(s) 1088, 1828, 2871, 3196, 3682
AvrII CCTAGG 2 cut(s) 1343, 2813
AxyI CCTNAGG 2 cut(s) 2243, 3450
BaeGI GKGCMC 4 cut(s) 268, 1894, 2141, 2770
BamHI GGATCC 1 cut(s) 2848
BanI GGYRCC 2 cut(s) 1929, 2765
BanII GRGCYC 3 cut(s) 1494, 1708, 4122
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 1 cut(s) 3978
BbsI GAAGAC 4 cut(s) 1946, 2433, 3115, 3183
Bbv12I GWGCWC 4 cut(s) 268, 1111, 2141, 4122
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 4 cut(s) 1337, 1420, 2190, 2470
BcgI CGANNNNNNTGC 2 cut(s) 3126, 3160
BclI TGATCA 4 cut(s) 1975, 2332, 2893, 3355
BcnI CCSGG 1 cut(s) 2763
BcoDI GTCTC 7 cut(s) 54, 329, 406, 516, 3597, 3898, 3942
BcuI ACTAGT 3 cut(s) 268, 3236, 3889
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 3673
BglII AGATCT 1 cut(s) 2003
BisI GCNGC 6 cut(s) 875, 1326, 1434, 2179, 2312, 2484
BlnI CCTAGG 2 cut(s) 1343, 2813
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 6 cut(s) 876, 1327, 1435, 2180, 2313, 2485
Bme18I GGWCC 5 cut(s) 1088, 1828, 2871, 3196, 3682
BmeRI GACNNNNNGTC 2 cut(s) 2075, 3184
BmeT110I CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
BmgBI CACGTC 2 cut(s) 501, 1783
BmgT120I GGNCC 7 cut(s) 1088, 1828, 2871, 3196, 3317, 3394, 3682
BmiI GGNNCC 9 cut(s) 6, 381, 1493, 1707, 1931, 2767, 2811, 2850, 2873
BpiI GAAGAC 4 cut(s) 1946, 2433, 3115, 3183
BplI GAGNNNNNCTC 5 cut(s) 386, 418, 496, 528, 4117
BpmI CTGGAG 4 cut(s) 1230, 1377, 1478, 1952
Bpu10I CCTNAGC 2 cut(s) 78, 174
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 2 cut(s) 416, 3587
BpuEI CTTGAG 2 cut(s) 2050, 4106
BpuMI CCSGG 1 cut(s) 2763
BsaBI GATNNNNATC 2 cut(s) 2676, 3360
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 9 cut(s) 451, 863, 1246, 1343, 1608, 2642, 2813, 3798, 3865
BsaWI WCCGGW 4 cut(s) 226, 406, 514, 1811
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bse1I ACTGG 9 cut(s) 971, 1032, 1954, 1969, 2261, 3305, 3475, 3635, 3956
Bse21I CCTNAGG 2 cut(s) 2243, 3450
Bse3DI GCAATG 6 cut(s) 887, 1200, 1384, 3142, 3162, 3464
Bse8I GATNNNNATC 2 cut(s) 2676, 3360
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 9 cut(s) 451, 863, 1246, 1343, 1608, 2642, 2813, 3798, 3865
BseJI GATNNNNATC 2 cut(s) 2676, 3360
BseMI GCAATG 6 cut(s) 887, 1200, 1384, 3142, 3162, 3464
BseNI ACTGG 9 cut(s) 971, 1032, 1954, 1969, 2261, 3305, 3475, 3635, 3956
BseRI GAGGAG 4 cut(s) 153, 410, 1706, 3363
BseSI GKGCMC 4 cut(s) 268, 1894, 2141, 2770
BseXI GCAGC 4 cut(s) 1337, 1420, 2190, 2470
BsgI GTGCAG 2 cut(s) 1419, 2131
Bsh1236I CGCG 1 cut(s) 4014
BshFI GGCC 5 cut(s) 874, 2063, 2314, 3319, 3395
BshNI GGYRCC 2 cut(s) 1929, 2765
BsiHKAI GWGCWC 4 cut(s) 268, 1111, 2141, 4122
BsiHKCI CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
BsiSI CCGG 5 cut(s) 227, 407, 515, 1812, 2762
BslFI GGGAC 5 cut(s) 661, 1553, 2267, 2857, 3225
BsmAI GTCTC 7 cut(s) 54, 329, 406, 516, 3597, 3898, 3942
BsmBI CGTCTC 2 cut(s) 54, 516
BsmFI GGGAC 5 cut(s) 661, 1553, 2267, 2857, 3225
BsmI GAATGC 2 cut(s) 2485, 2958
BsnI GGCC 5 cut(s) 874, 2063, 2314, 3319, 3395
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
Bsp119I TTCGAA 2 cut(s) 416, 3587
Bsp1286I GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1894, 2141, 2770, 4122
Bsp13I TCCGGA 1 cut(s) 226
Bsp1720I GCTNAGC 1 cut(s) 72
BspANI GGCC 5 cut(s) 874, 2063, 2314, 3319, 3395
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 4014
BspHI TCATGA 3 cut(s) 814, 1093, 1531
BspLI GGNNCC 9 cut(s) 6, 381, 1493, 1707, 1931, 2767, 2811, 2850, 2873
BspMI ACCTGC 1 cut(s) 3673
BspPI GGATC 7 cut(s) 226, 1272, 1648, 2843, 2856, 3239, 3553
BspQI GCTCTTC 1 cut(s) 1152
BspT104I TTCGAA 2 cut(s) 416, 3587
BspT107I GGYRCC 2 cut(s) 1929, 2765
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 6 cut(s) 887, 1200, 1384, 3142, 3162, 3464
BsrI ACTGG 9 cut(s) 971, 1032, 1954, 1969, 2261, 3305, 3475, 3635, 3956
BssECI CCNNGG 9 cut(s) 451, 863, 1246, 1343, 1608, 2642, 2813, 3798, 3865
BssNAI GTATAC 1 cut(s) 1546
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 3978
BssT1I CCWWGG 3 cut(s) 1246, 1343, 2813
Bst1107I GTATAC 1 cut(s) 1546
Bst2BI CACGAG 1 cut(s) 3978
Bst6I CTCTTC 8 cut(s) 160, 692, 1152, 1176, 1917, 2321, 3377, 3865
BstACI GRCGYC 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 2 cut(s) 416, 3587
BstC8I GCNNGC 8 cut(s) 617, 993, 1164, 2430, 2988, 3249, 3431, 3819
BstEII GGTNACC 1 cut(s) 2528
BstENI CCTNNNNNAGG 2 cut(s) 2961, 3901
BstFNI CGCG 1 cut(s) 4014
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 117, 3102, 3445
BstMAI GTCTC 7 cut(s) 54, 329, 406, 516, 3597, 3898, 3942
BstNSI RCATGY 6 cut(s) 995, 1120, 1524, 1943, 2990, 3225
BstPI GGTNACC 1 cut(s) 2528
BstSLI GKGCMC 4 cut(s) 268, 1894, 2141, 2770
BstUI CGCG 1 cut(s) 4014
BstV1I GCAGC 4 cut(s) 1337, 1420, 2190, 2470
BstV2I GAAGAC 4 cut(s) 1946, 2433, 3115, 3183
BstX2I RGATCY 5 cut(s) 231, 1640, 2003, 2848, 3545
BstYI RGATCY 5 cut(s) 231, 1640, 2003, 2848, 3545
BstZ17I GTATAC 1 cut(s) 1546
Bsu36I CCTNAGG 2 cut(s) 2243, 3450
BsuRI GGCC 5 cut(s) 874, 2063, 2314, 3319, 3395
BtrI CACGTC 2 cut(s) 501, 1783
BtsI GCAGTG 1 cut(s) 2554
BtsIMutI CAGTG 6 cut(s) 1118, 1201, 1259, 2554, 2830, 3757
BveI ACCTGC 1 cut(s) 3673
Cac8I GCNNGC 8 cut(s) 617, 993, 1164, 2430, 2988, 3249, 3431, 3819
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 3 cut(s) 814, 1093, 1531
CfoI GCGC 3 cut(s) 117, 3102, 3445
Cfr13I GGNCC 7 cut(s) 1088, 1828, 2871, 3196, 3317, 3394, 3682
CseI GACGC 1 cut(s) 2888
Csp6I GTAC 6 cut(s) 327, 941, 1850, 1930, 3387, 3478
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 2304, 2339
CviQI GTAC 6 cut(s) 327, 941, 1850, 1930, 3387, 3478
DraI TTTAAA 3 cut(s) 1807, 2886, 3621
DriI GACNNNNNGTC 2 cut(s) 2075, 3184
EaeI YGGCCR 1 cut(s) 2312
Eam1104I CTCTTC 8 cut(s) 160, 692, 1152, 1176, 1917, 2321, 3377, 3865
Eam1105I GACNNNNNGTC 2 cut(s) 2075, 3184
EarI CTCTTC 8 cut(s) 160, 692, 1152, 1176, 1917, 2321, 3377, 3865
EciI GGCGGA 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 4120
Eco130I CCWWGG 3 cut(s) 1246, 1343, 2813
Eco147I AGGCCT 1 cut(s) 2063
Eco24I GRGCYC 3 cut(s) 1494, 1708, 4122
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 5 cut(s) 1088, 1828, 2871, 3196, 3682
Eco53kI GAGCTC 1 cut(s) 4120
Eco57I CTGAAG 2 cut(s) 1638, 2643
Eco81I CCTNAGG 2 cut(s) 2243, 3450
Eco88I CYCGRG 5 cut(s) 287, 1317, 1878, 3314, 3396
Eco91I GGTNACC 1 cut(s) 2528
EcoICRI GAGCTC 1 cut(s) 4120
EcoNI CCTNNNNNAGG 2 cut(s) 2961, 3901
EcoO65I GGTNACC 1 cut(s) 2528
EcoT14I CCWWGG 3 cut(s) 1246, 1343, 2813
EcoT22I ATGCAT 2 cut(s) 1522, 3275
EcoT38I GRGCYC 3 cut(s) 1494, 1708, 4122
ErhI CCWWGG 3 cut(s) 1246, 1343, 2813
Esp3I CGTCTC 2 cut(s) 54, 516
FaqI GGGAC 5 cut(s) 661, 1553, 2267, 2857, 3225
FauI CCCGC 1 cut(s) 2685
FbaI TGATCA 4 cut(s) 1975, 2332, 2893, 3355
FblI GTMKAC 1 cut(s) 1545
Fnu4HI GCNGC 6 cut(s) 875, 1326, 1434, 2179, 2312, 2484
FriOI GRGCYC 3 cut(s) 1494, 1708, 4122
Fsp4HI GCNGC 6 cut(s) 875, 1326, 1434, 2179, 2312, 2484
FspAI RTGCGCAY 1 cut(s) 3101
FspI TGCGCA 1 cut(s) 3101
GlaI GCGC 3 cut(s) 116, 3101, 3444
GluI GCNGC 6 cut(s) 875, 1326, 1434, 2179, 2312, 2484
GsuI CTGGAG 4 cut(s) 1230, 1377, 1478, 1952
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 5 cut(s) 874, 2063, 2314, 3319, 3395
HapII CCGG 5 cut(s) 227, 407, 515, 1812, 2762
HgaI GACGC 1 cut(s) 2888
HhaI GCGC 3 cut(s) 117, 3102, 3445
Hin1I GRCGYC 1 cut(s) 509
Hin6I GCGC 3 cut(s) 115, 3100, 3443
HinP1I GCGC 3 cut(s) 115, 3100, 3443
HincII GTYRAC 5 cut(s) 1222, 2698, 2800, 3748, 3909
HindII GTYRAC 5 cut(s) 1222, 2698, 2800, 3748, 3909
HindIII AAGCTT 5 cut(s) 821, 1620, 2104, 3418, 4043
HpaI GTTAAC 1 cut(s) 3748
HpaII CCGG 5 cut(s) 227, 407, 515, 1812, 2762
Hpy99I CGWCG 2 cut(s) 673, 2904
HpyCH4IV ACGT 8 cut(s) 500, 509, 1224, 1553, 1782, 2032, 2837, 4086
HpySE526I ACGT 8 cut(s) 500, 509, 1224, 1553, 1782, 2032, 2837, 4086
Hsp92I GRCGYC 1 cut(s) 509
HspAI GCGC 3 cut(s) 115, 3100, 3443
Kpn2I TCCGGA 1 cut(s) 226
KpnI GGTACC 1 cut(s) 1933
Ksp22I TGATCA 4 cut(s) 1975, 2332, 2893, 3355
KspAI GTTAAC 1 cut(s) 3748
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 8 cut(s) 10, 379, 1021, 1497, 1711, 2287, 2815, 4117
Lsp1109I GCAGC 4 cut(s) 1337, 1420, 2190, 2470
MaeII ACGT 8 cut(s) 500, 509, 1224, 1553, 1782, 2032, 2837, 4086
MflI RGATCY 5 cut(s) 231, 1640, 2003, 2848, 3545
MhlI GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1894, 2141, 2770, 4122
MlyI GAGTC 4 cut(s) 185, 481, 787, 2107
MmeI TCCRAC 3 cut(s) 124, 1043, 1902
Mph1103I ATGCAT 2 cut(s) 1522, 3275
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 2 cut(s) 1595, 3132
MslI CAYNNNNRTG 5 cut(s) 1296, 1536, 2130, 2521, 2604
MspA1I CMGCKG 1 cut(s) 2181
MspI CCGG 5 cut(s) 227, 407, 515, 1812, 2762
Mva1269I GAATGC 2 cut(s) 2485, 2958
MvnI CGCG 1 cut(s) 4014
NciI CCSGG 1 cut(s) 2763
NlaIV GGNNCC 9 cut(s) 6, 381, 1493, 1707, 1931, 2767, 2811, 2850, 2873
NmuCI GTSAC 7 cut(s) 496, 1009, 1663, 2528, 2597, 3527, 4008
NsbI TGCGCA 1 cut(s) 3101
NsiI ATGCAT 2 cut(s) 1522, 3275
NspI RCATGY 6 cut(s) 995, 1120, 1524, 1943, 2990, 3225
NspV TTCGAA 2 cut(s) 416, 3587
PaeI GCATGC 2 cut(s) 995, 2990
PaeR7I CTCGAG 1 cut(s) 1878
PagI TCATGA 3 cut(s) 814, 1093, 1531
PceI AGGCCT 1 cut(s) 2063
PciSI GCTCTTC 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 2 cut(s) 506, 4011
PctI GAATGC 2 cut(s) 2485, 2958
PdmI GAANNNNTTC 2 cut(s) 1595, 3132
PflFI GACNNNGTC 2 cut(s) 1254, 3200
PflMI CCANNNNNTGG 1 cut(s) 1715
PkrI GCNGC 6 cut(s) 876, 1327, 1435, 2180, 2313, 2485
PleI GAGTC 4 cut(s) 185, 480, 786, 2106
PpsI GAGTC 4 cut(s) 185, 480, 786, 2106
PshBI ATTAAT 2 cut(s) 3648, 3963
Psp124BI GAGCTC 1 cut(s) 4122
Psp1406I AACGTT 2 cut(s) 1224, 1553
PspEI GGTNACC 1 cut(s) 2528
PspN4I GGNNCC 9 cut(s) 6, 381, 1493, 1707, 1931, 2767, 2811, 2850, 2873
PspPI GGNCC 7 cut(s) 1088, 1828, 2871, 3196, 3317, 3394, 3682
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 5 cut(s) 231, 1640, 2003, 2848, 3545
PsyI GACNNNGTC 2 cut(s) 1254, 3200
RsaI GTAC 6 cut(s) 328, 942, 1851, 1931, 3388, 3479
RsaNI GTAC 6 cut(s) 327, 941, 1850, 1930, 3387, 3478
RseI CAYNNNNRTG 5 cut(s) 1296, 1536, 2130, 2521, 2604
SacI GAGCTC 1 cut(s) 4122
SapI GCTCTTC 1 cut(s) 1152
SatI GCNGC 6 cut(s) 875, 1326, 1434, 2179, 2312, 2484
Sau96I GGNCC 7 cut(s) 1088, 1828, 2871, 3196, 3317, 3394, 3682
SchI GAGTC 4 cut(s) 185, 481, 787, 2107
SduI GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1894, 2141, 2770, 4122
Sfr274I CTCGAG 1 cut(s) 1878
SfuI TTCGAA 2 cut(s) 416, 3587
SinI GGWCC 5 cut(s) 1088, 1828, 2871, 3196, 3682
SlaI CTCGAG 1 cut(s) 1878
SmiI ATTTAAAT 1 cut(s) 2886
SmiMI CAYNNNNRTG 5 cut(s) 1296, 1536, 2130, 2521, 2604
SmlI CTYRAG 3 cut(s) 1878, 2065, 4121
SmoI CTYRAG 3 cut(s) 1878, 2065, 4121
SpeI ACTAGT 3 cut(s) 268, 3236, 3889
SphI GCATGC 2 cut(s) 995, 2990
SseBI AGGCCT 1 cut(s) 2063
SspI AATATT 3 cut(s) 1413, 1578, 3339
SstI GAGCTC 1 cut(s) 4122
StuI AGGCCT 1 cut(s) 2063
StyI CCWWGG 3 cut(s) 1246, 1343, 2813
SwaI ATTTAAAT 1 cut(s) 2886
TaiI ACGT 8 cut(s) 503, 512, 1227, 1556, 1785, 2035, 2840, 4089
TatI WGTACW 1 cut(s) 3386
TauI GCSGC 2 cut(s) 877, 2314
TscAI CASTG 6 cut(s) 1118, 1201, 1259, 2554, 2830, 3757
TseFI GTSAC 7 cut(s) 496, 1009, 1663, 2528, 2597, 3527, 4008
TseI GCWGC 4 cut(s) 1325, 1433, 2178, 2483
Tsp45I GTSAC 7 cut(s) 496, 1009, 1663, 2528, 2597, 3527, 4008
TspGWI ACGGA 1 cut(s) 2110
TspRI CASTG 6 cut(s) 1118, 1201, 1259, 2554, 2830, 3757
Tth111I GACNNNGTC 2 cut(s) 1254, 3200
Van91I CCANNNNNTGG 1 cut(s) 1715
VneI GTGCAC 2 cut(s) 264, 2137
VpaK11BI GGWCC 5 cut(s) 1088, 1828, 2871, 3196, 3682
VspI ATTAAT 2 cut(s) 3648, 3963
XagI CCTNNNNNAGG 2 cut(s) 2961, 3901
XapI RAATTY 7 cut(s) 349, 1235, 2408, 2708, 2881, 3043, 3260
XceI RCATGY 6 cut(s) 995, 1120, 1524, 1943, 2990, 3225
XhoI CTCGAG 1 cut(s) 1878
XmaJI CCTAGG 2 cut(s) 1343, 2813
XmiI GTMKAC 1 cut(s) 1545
XmnI GAANNNNTTC 2 cut(s) 1595, 3132
ZraI GACGTC 1 cut(s) 510
Zsp2I ATGCAT 2 cut(s) 1522, 3275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.