RLG00000001791

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002244
Physical Location & Seq
Reverse (-)
20839225 .. 20839879
655 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001791

Sequence Viewer

Length: 396 bp
ATGATGAGAGTATTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGCCAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGAGAAATCCCTATCGTCGGTGTCCCTGAATAGGGTTGGTGTGAAGAGGGTTAAACCGGATCAGGATGTGGAACATCATGGAAATGTGGTTGATAGGACTATTTCTGAAATAGCTGGTAGTCAGAGTCCATTTCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTGCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCAGCACAGGGCAGAAACCCTAAGCAGTAGGACATGTCAGTTCATGCTCTTCAGATTTTCTGATGGGTTTGATCACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

132

Amino Acids

14.41

Weight (kDa)

8.34

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 349
AfiI CCNNNNNNNGG 3 cut(s) 112, 126, 127
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 2 cut(s) 27, 83
AjnI CCWGG 1 cut(s) 304
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 3 cut(s) 86, 209, 265
AluI AGCT 3 cut(s) 86, 209, 265
AlwI GGATC 1 cut(s) 162
ApeKI GCWGC 1 cut(s) 316
AsuII TTCGAA 1 cut(s) 229
BbvI GCAGC 1 cut(s) 328
BccI CCATC 2 cut(s) 281, 371
BciT130I CCWGG 1 cut(s) 306
BclI TGATCA 1 cut(s) 385
BisI GCNGC 1 cut(s) 317
BlsI GCNGC 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 335
Bpu14I TTCGAA 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 126, 127
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 3 cut(s) 112, 126, 127
BseXI GCAGC 1 cut(s) 328
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 3 cut(s) 112, 126, 127
BsmFI GGGAC 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 229
Bsp143I GATC 2 cut(s) 154, 385
BspHI TCATGA 1 cut(s) 256
BspPI GGATC 1 cut(s) 162
BspQI GCTCTTC 1 cut(s) 368
BspT104I TTCGAA 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 2 cut(s) 154, 385
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 254, 392
Bst6I CTCTTC 2 cut(s) 134, 368
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 59, 267
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 2 cut(s) 157, 388
BstMBI GATC 2 cut(s) 154, 385
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 2 cut(s) 59, 267
CciI TCATGA 1 cut(s) 256
CviAII CATG 5 cut(s) 173, 257, 282, 348, 358
CviJI RGCY 5 cut(s) 31, 86, 209, 265, 304
CviKI_1 RGCY 5 cut(s) 31, 86, 209, 265, 304
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 156, 387
DpnII GATC 2 cut(s) 154, 385
Eam1104I CTCTTC 2 cut(s) 134, 368
EarI CTCTTC 2 cut(s) 134, 368
Eco57I CTGAAG 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 304
FaeI CATG 5 cut(s) 176, 260, 285, 351, 361
FaiI YATR 6 cut(s) 174, 250, 258, 283, 349, 359
FaqI GGGAC 1 cut(s) 103
FatI CATG 5 cut(s) 172, 256, 281, 347, 357
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 317
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 317
HapII CCGG 1 cut(s) 152
Hin1II CATG 5 cut(s) 176, 260, 285, 351, 361
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 1 cut(s) 220
HpaII CCGG 1 cut(s) 152
Hpy188I TCNGA 5 cut(s) 202, 219, 298, 368, 376
Hpy188III TCNNGA 2 cut(s) 158, 257
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 249
HpyCH4III ACNGT 2 cut(s) 254, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 335
Hsp92II CATG 5 cut(s) 176, 260, 285, 351, 361
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 2 cut(s) 154, 385
LguI GCTCTTC 1 cut(s) 368
LpnPI CCDG 8 cut(s) 72, 134, 143, 165, 195, 291, 307, 318
Lsp1109I GCAGC 1 cut(s) 328
MalI GATC 2 cut(s) 156, 387
MboI GATC 2 cut(s) 154, 385
MboII GAAGA 2 cut(s) 151, 355
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 2 cut(s) 135, 306
MseI TTAA 3 cut(s) 41, 48, 147
MslI CAYNNNNRTG 1 cut(s) 177
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 2 cut(s) 154, 385
NlaIII CATG 5 cut(s) 176, 260, 285, 351, 361
NspI RCATGY 1 cut(s) 351
NspV TTCGAA 1 cut(s) 229
PagI TCATGA 1 cut(s) 256
PciI ACATGT 1 cut(s) 347
PciSI GCTCTTC 1 cut(s) 368
PkrI GCNGC 1 cut(s) 318
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PscI ACATGT 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 41, 48, 147
SatI GCNGC 1 cut(s) 317
Sau3AI GATC 2 cut(s) 154, 385
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 1 cut(s) 306
SetI ASST 5 cut(s) 88, 96, 211, 241, 267
SfuI TTCGAA 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 177
StyD4I CCNGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 254, 392
TaqI TCGA 1 cut(s) 229
Tru1I TTAA 3 cut(s) 41, 48, 147
Tru9I TTAA 3 cut(s) 41, 48, 147
TscAI CASTG 1 cut(s) 395
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 270, 346
TspRI CASTG 1 cut(s) 395
XceI RCATGY 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.