Rw1G015060

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002244
Physical Location & Seq
Reverse (-)
33411307 .. 33418486
7180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015060.1

Sequence Viewer

Length: 2043 bp
ATGAAGGAGAAACCCACAATTACTTTCTTGGAGGAACATGCAAGAATGGGGCTCCAGAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTGAACATCTTCAAACACACCCAGAATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAGGTCACAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTCGAAGCTAACAAAAATGAATCTGACGTGAAGGTTATGCCTTTGGAGTTTAAAACCGGGAAAGTGCCGAAGGACCAGGAGCATTGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAGAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACGGAGTCCAAGCTTGTGCAGAAGTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGGCACATGGAGGCAACACAGATAGCAGCGGATTGGGAGATTTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGACTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGCTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGTGATCATTCCACAAGTTGCCTTTCTTCTATGGTTCTTGACAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTGCTGGTTTGTCTTCTCTCAATGTGAGGACATCCAATGGAGATTTTCAGAATGCTGCTTCTTCACCCTCAAAATATGCAGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTAGCTGTTGCAAGGGGGGTCATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGGGCAATCCTTCTTCAGAGGCAAGTGATCTTTATCGGGAGGTCTGGCGGGTTGACAAAGATGAATTTCTGACATCATTTGCCACAATGAGGTTCAATCTTCTTCAACTTTTTCTTCCGGGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGGTTTGACAGTGGATGTATTACGTTCAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTCGTGCCATTCTAAAGATACTTACAGCGAAGGACTACGCTCTTATTCTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGCGAGGTGCATCAATTTTGCTTACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTGCGCATTGGAAGACAGGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCCTTTCAGCAATGAACATACAAAGTGTGGAAGACATTAAGTCAAGATTGGACCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAACAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTCGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCCCGAAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCACATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGTATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

680

Amino Acids

75.84

Weight (kDa)

8.74

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 3 - 104 1.1e-22 DNA replication factor Dna2
AAA_11 PF13086 465 - 557 2.7e-17 AAA domain
AAA_30 PF13604 466 - 629 8.9e-10 AAA domain
UvrD-helicase PF00580 466 - 562 5.6e-06 UvrD/REP helicase N-terminal domain
AAA_19 PF13245 470 - 629 1.7e-07 AAA domain
AAA_11 PF13086 566 - 633 2.8e-16 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1613
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 2 cut(s) 441, 1277
AccB7I CCANNNNNTGG 1 cut(s) 275
AccI GTMKAC 1 cut(s) 105
AciI CCGC 4 cut(s) 506, 693, 823, 1204
AclI AACGTT 1 cut(s) 113
AclWI GGATC 4 cut(s) 208, 1355, 1368, 1751
AcoI YGGCCR 1 cut(s) 824
AcsI RAATTY 6 cut(s) 172, 920, 1220, 1393, 1555, 1772
AcuI CTGAAG 2 cut(s) 198, 1155
AfaI GTAC 3 cut(s) 443, 1900, 1992
AfiI CCNNNNNNNGG 5 cut(s) 275, 1245, 1380, 1475, 1717
AgsI TTSAA 7 cut(s) 160, 717, 838, 900, 1252, 1262, 1848
AhdI GACNNNNNGTC 2 cut(s) 587, 1696
AhlI ACTAGT 1 cut(s) 1748
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 5 cut(s) 390, 1153, 1356, 1468, 1857
Alw21I GWGCWC 1 cut(s) 653
Alw44I GTGCAC 1 cut(s) 649
AlwI GGATC 4 cut(s) 208, 1355, 1368, 1751
Ama87I CYCGRG 1 cut(s) 1826
AoxI GGCC 4 cut(s) 573, 824, 1829, 1905
ApaLI GTGCAC 1 cut(s) 649
ApeKI GCWGC 2 cut(s) 690, 995
ApoI RAATTY 6 cut(s) 172, 920, 1220, 1393, 1555, 1772
Asp700I GAANNNNTTC 2 cut(s) 155, 1644
Asp718I GGTACC 1 cut(s) 441
AspA2I CCTAGG 1 cut(s) 1325
AspLEI GCGC 1 cut(s) 1614
AspS9I GGNCC 5 cut(s) 388, 1383, 1708, 1829, 1906
AsuC2I CCSGG 2 cut(s) 373, 1275
AsuHPI GGTGA 8 cut(s) 160, 226, 322, 716, 996, 1052, 1877, 1903
AvaI CYCGRG 1 cut(s) 1826
AvaII GGWCC 3 cut(s) 388, 1383, 1708
AvrII CCTAGG 1 cut(s) 1325
AxyI CCTNAGG 2 cut(s) 755, 1963
BaeGI GKGCMC 3 cut(s) 406, 653, 1282
BamHI GGATCC 1 cut(s) 1360
BanI GGYRCC 2 cut(s) 441, 1277
BanII GRGCYC 2 cut(s) 54, 268
BbsI GAAGAC 4 cut(s) 458, 945, 1627, 1695
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 2 cut(s) 702, 982
BccI CCATC 4 cut(s) 250, 645, 662, 790
BcgI CGANNNNNNTGC 2 cut(s) 1638, 1672
BciT130I CCWGG 5 cut(s) 392, 1155, 1358, 1470, 1859
BclI TGATCA 4 cut(s) 487, 844, 1405, 1867
BcnI CCSGG 2 cut(s) 373, 1275
BcuI ACTAGT 1 cut(s) 1748
BfaI CTAG 5 cut(s) 1076, 1316, 1326, 1460, 1749
BglII AGATCT 1 cut(s) 515
BisI GCNGC 3 cut(s) 691, 824, 996
BlnI CCTAGG 1 cut(s) 1325
BlsI GCNGC 3 cut(s) 692, 825, 997
Bme1390I CCNGG 7 cut(s) 373, 392, 1155, 1275, 1358, 1470, 1859
Bme18I GGWCC 3 cut(s) 388, 1383, 1708
BmeRI GACNNNNNGTC 2 cut(s) 587, 1696
BmeT110I CYCGRG 1 cut(s) 1826
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 5 cut(s) 388, 1383, 1708, 1829, 1906
BmiI GGNNCC 7 cut(s) 53, 267, 443, 1279, 1323, 1362, 1385
BmrFI CCNGG 7 cut(s) 373, 392, 1155, 1275, 1358, 1470, 1859
BmsI GCATC 5 cut(s) 112, 286, 1509, 1538, 1794
BpiI GAAGAC 4 cut(s) 458, 945, 1627, 1695
BpmI CTGGAG 2 cut(s) 38, 464
BpuEI CTTGAG 1 cut(s) 562
BpuMI CCSGG 2 cut(s) 373, 1275
BsaBI GATNNNNATC 2 cut(s) 1188, 1872
BsaJI CCNNGG 2 cut(s) 1154, 1325
Bsc4I CCNNNNNNNGG 5 cut(s) 275, 1245, 1380, 1475, 1717
Bse1I ACTGG 5 cut(s) 466, 481, 773, 1817, 1988
Bse21I CCTNAGG 2 cut(s) 755, 1963
Bse3DI GCAATG 3 cut(s) 1654, 1674, 1977
Bse8I GATNNNNATC 2 cut(s) 1188, 1872
BseBI CCWGG 5 cut(s) 392, 1155, 1358, 1470, 1859
BseDI CCNNGG 2 cut(s) 1154, 1325
BseGI GGATG 4 cut(s) 971, 1346, 1798, 1958
BseJI GATNNNNATC 2 cut(s) 1188, 1872
BseLI CCNNNNNNNGG 5 cut(s) 275, 1245, 1380, 1475, 1717
BseMI GCAATG 3 cut(s) 1654, 1674, 1977
BseMII CTCAG 6 cut(s) 195, 225, 1286, 1607, 1963, 1977
BseNI ACTGG 5 cut(s) 466, 481, 773, 1817, 1988
BseRI GAGGAG 2 cut(s) 266, 1875
BseSI GKGCMC 3 cut(s) 406, 653, 1282
BseXI GCAGC 2 cut(s) 702, 982
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 4 cut(s) 575, 826, 1831, 1907
BshNI GGYRCC 2 cut(s) 441, 1277
BsiHKAI GWGCWC 1 cut(s) 653
BsiHKCI CYCGRG 1 cut(s) 1826
BsiSI CCGG 2 cut(s) 372, 1274
BslFI GGGAC 4 cut(s) 113, 779, 1369, 1737
BslI CCNNNNNNNGG 5 cut(s) 275, 1245, 1380, 1475, 1717
BsmFI GGGAC 4 cut(s) 113, 779, 1369, 1737
BsmI GAATGC 2 cut(s) 997, 1470
BsnI GGCC 4 cut(s) 575, 826, 1831, 1907
BsoBI CYCGRG 1 cut(s) 1826
Bsp1286I GDGCHC 5 cut(s) 54, 268, 406, 653, 1282
BspACI CCGC 4 cut(s) 506, 693, 823, 1204
BspANI GGCC 4 cut(s) 575, 826, 1831, 1907
BspCNI CTCAG 6 cut(s) 196, 226, 1287, 1606, 1962, 1976
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 7 cut(s) 53, 267, 443, 1279, 1323, 1362, 1385
BspPI GGATC 4 cut(s) 208, 1355, 1368, 1751
BspT107I GGYRCC 2 cut(s) 441, 1277
BsrDI GCAATG 3 cut(s) 1654, 1674, 1977
BsrI ACTGG 5 cut(s) 466, 481, 773, 1817, 1988
BssECI CCNNGG 2 cut(s) 1154, 1325
BssNAI GTATAC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 1325
Bst1107I GTATAC 1 cut(s) 106
Bst2UI CCWGG 5 cut(s) 392, 1155, 1358, 1470, 1859
Bst4CI ACNGT 4 cut(s) 932, 1337, 1995, 2020
Bst6I CTCTTC 3 cut(s) 429, 833, 1889
BstC8I GCNNGC 4 cut(s) 942, 1500, 1761, 1944
BstDEI CTNAG 9 cut(s) 204, 234, 755, 1031, 1137, 1295, 1593, 1949, 1963
BstEII GGTNACC 1 cut(s) 1040
BstENI CCTNNNNNAGG 1 cut(s) 1473
BstF5I GGATG 4 cut(s) 971, 1346, 1798, 1958
BstHHI GCGC 1 cut(s) 1614
BstMWI GCNNNNNNNGC 5 cut(s) 538, 1286, 1508, 1939, 1974
BstNI CCWGG 5 cut(s) 392, 1155, 1358, 1470, 1859
BstNSI RCATGY 5 cut(s) 41, 84, 455, 1502, 1737
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 7 cut(s) 371, 390, 1153, 1273, 1356, 1468, 1857
BstSLI GKGCMC 3 cut(s) 406, 653, 1282
BstV1I GCAGC 2 cut(s) 702, 982
BstV2I GAAGAC 4 cut(s) 458, 945, 1627, 1695
BstX2I RGATCY 3 cut(s) 200, 515, 1360
BstYI RGATCY 3 cut(s) 200, 515, 1360
BstZ17I GTATAC 1 cut(s) 106
Bsu36I CCTNAGG 2 cut(s) 755, 1963
BsuRI GGCC 4 cut(s) 575, 826, 1831, 1907
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 4 cut(s) 971, 1346, 1798, 1958
BtsI GCAGTG 1 cut(s) 1066
BtsIMutI CAGTG 2 cut(s) 1066, 1342
Cac8I GCNNGC 4 cut(s) 942, 1500, 1761, 1944
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 1614
Cfr13I GGNCC 5 cut(s) 388, 1383, 1708, 1829, 1906
CseI GACGC 1 cut(s) 1400
Csp6I GTAC 3 cut(s) 442, 1899, 1991
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviQI GTAC 3 cut(s) 442, 1899, 1991
DdeI CTNAG 9 cut(s) 204, 234, 755, 1031, 1137, 1295, 1593, 1949, 1963
DraI TTTAAA 2 cut(s) 367, 1398
DriI GACNNNNNGTC 2 cut(s) 587, 1696
EaeI YGGCCR 1 cut(s) 824
Eam1104I CTCTTC 3 cut(s) 429, 833, 1889
Eam1105I GACNNNNNGTC 2 cut(s) 587, 1696
EarI CTCTTC 3 cut(s) 429, 833, 1889
Eco130I CCWWGG 1 cut(s) 1325
Eco147I AGGCCT 1 cut(s) 575
Eco24I GRGCYC 2 cut(s) 54, 268
Eco47I GGWCC 3 cut(s) 388, 1383, 1708
Eco57I CTGAAG 2 cut(s) 198, 1155
Eco81I CCTNAGG 2 cut(s) 755, 1963
Eco88I CYCGRG 1 cut(s) 1826
Eco91I GGTNACC 1 cut(s) 1040
EcoNI CCTNNNNNAGG 1 cut(s) 1473
EcoO65I GGTNACC 1 cut(s) 1040
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 5 cut(s) 390, 1153, 1356, 1468, 1857
EcoT14I CCWWGG 1 cut(s) 1325
EcoT22I ATGCAT 2 cut(s) 82, 1787
EcoT38I GRGCYC 2 cut(s) 54, 268
ErhI CCWWGG 1 cut(s) 1325
FaqI GGGAC 4 cut(s) 113, 779, 1369, 1737
FauI CCCGC 1 cut(s) 1197
FbaI TGATCA 4 cut(s) 487, 844, 1405, 1867
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 691, 824, 996
FokI GGATG 4 cut(s) 958, 1353, 1805, 1945
FriOI GRGCYC 2 cut(s) 54, 268
Fsp4HI GCNGC 3 cut(s) 691, 824, 996
FspAI RTGCGCAY 1 cut(s) 1613
FspBI CTAG 5 cut(s) 1076, 1316, 1326, 1460, 1749
FspI TGCGCA 1 cut(s) 1613
GlaI GCGC 1 cut(s) 1613
GluI GCNGC 3 cut(s) 691, 824, 996
GsuI CTGGAG 2 cut(s) 38, 464
HaeIII GGCC 4 cut(s) 575, 826, 1831, 1907
HapII CCGG 2 cut(s) 372, 1274
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 1 cut(s) 1614
Hin6I GCGC 1 cut(s) 1612
HinP1I GCGC 1 cut(s) 1612
HincII GTYRAC 2 cut(s) 1210, 1312
HindII GTYRAC 2 cut(s) 1210, 1312
HindIII AAGCTT 3 cut(s) 180, 616, 1931
HinfI GANTC 4 cut(s) 335, 610, 713, 1064
HpaII CCGG 2 cut(s) 372, 1274
HphI GGTGA 8 cut(s) 160, 226, 322, 716, 996, 1052, 1877, 1903
Hpy166II GTNNAC 5 cut(s) 106, 151, 651, 1210, 1312
Hpy188III TCNNGA 7 cut(s) 55, 92, 579, 878, 906, 1193, 1701
Hpy8I GTNNAC 5 cut(s) 106, 151, 651, 1210, 1312
Hpy99I CGWCG 1 cut(s) 1416
HpyAV CCTTC 6 cut(s) 340, 379, 602, 1176, 1435, 1823
HpyCH4III ACNGT 4 cut(s) 932, 1337, 1995, 2020
HpyCH4IV ACGT 4 cut(s) 113, 342, 544, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 538, 1286, 1508, 1939, 1974
HpyF3I CTNAG 9 cut(s) 204, 234, 755, 1031, 1137, 1295, 1593, 1949, 1963
HpySE526I ACGT 4 cut(s) 113, 342, 544, 1349
HspAI GCGC 1 cut(s) 1612
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 4 cut(s) 487, 844, 1405, 1867
LmnI GCTCC 5 cut(s) 57, 271, 394, 799, 1327
Lsp1109I GCAGC 2 cut(s) 702, 982
LweI GCATC 5 cut(s) 112, 286, 1509, 1538, 1794
MaeI CTAG 5 cut(s) 1076, 1316, 1326, 1460, 1749
MaeII ACGT 4 cut(s) 113, 342, 544, 1349
MaeIII GTNAC 7 cut(s) 223, 473, 603, 1040, 1109, 1143, 1729
MflI RGATCY 3 cut(s) 200, 515, 1360
MhlI GDGCHC 5 cut(s) 54, 268, 406, 653, 1282
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 414
Mph1103I ATGCAT 2 cut(s) 82, 1787
MroXI GAANNNNTTC 2 cut(s) 155, 1644
MseI TTAA 5 cut(s) 140, 366, 1397, 1586, 1695
MslI CAYNNNNRTG 4 cut(s) 96, 642, 1033, 1116
MspA1I CMGCKG 1 cut(s) 693
MspI CCGG 2 cut(s) 372, 1274
MspR9I CCNGG 7 cut(s) 373, 392, 1155, 1275, 1358, 1470, 1859
Mva1269I GAATGC 2 cut(s) 997, 1470
MvaI CCWGG 5 cut(s) 392, 1155, 1358, 1470, 1859
MwoI GCNNNNNNNGC 5 cut(s) 538, 1286, 1508, 1939, 1974
NciI CCSGG 2 cut(s) 373, 1275
NlaIV GGNNCC 7 cut(s) 53, 267, 443, 1279, 1323, 1362, 1385
NmuCI GTSAC 3 cut(s) 223, 1040, 1109
NsbI TGCGCA 1 cut(s) 1613
NsiI ATGCAT 2 cut(s) 82, 1787
NspI RCATGY 5 cut(s) 41, 84, 455, 1502, 1737
PaeI GCATGC 1 cut(s) 1502
PagI TCATGA 1 cut(s) 91
PceI AGGCCT 1 cut(s) 575
PctI GAATGC 2 cut(s) 997, 1470
PdmI GAANNNNTTC 2 cut(s) 155, 1644
PfeI GAWTC 3 cut(s) 335, 713, 1064
PflFI GACNNNGTC 1 cut(s) 1712
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 3 cut(s) 692, 825, 997
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
Psp1406I AACGTT 1 cut(s) 113
Psp6I CCWGG 5 cut(s) 390, 1153, 1356, 1468, 1857
PspEI GGTNACC 1 cut(s) 1040
PspGI CCWGG 5 cut(s) 390, 1153, 1356, 1468, 1857
PspN4I GGNNCC 7 cut(s) 53, 267, 443, 1279, 1323, 1362, 1385
PspPI GGNCC 5 cut(s) 388, 1383, 1708, 1829, 1906
PsuI RGATCY 3 cut(s) 200, 515, 1360
PsyI GACNNNGTC 1 cut(s) 1712
RsaI GTAC 3 cut(s) 443, 1900, 1992
RsaNI GTAC 3 cut(s) 442, 1899, 1991
RseI CAYNNNNRTG 4 cut(s) 96, 642, 1033, 1116
SaqAI TTAA 5 cut(s) 140, 366, 1397, 1586, 1695
SatI GCNGC 3 cut(s) 691, 824, 996
Sau96I GGNCC 5 cut(s) 388, 1383, 1708, 1829, 1906
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 7 cut(s) 373, 392, 1155, 1275, 1358, 1470, 1859
SduI GDGCHC 5 cut(s) 54, 268, 406, 653, 1282
SfaNI GCATC 5 cut(s) 112, 286, 1509, 1538, 1794
SinI GGWCC 3 cut(s) 388, 1383, 1708
SmiI ATTTAAAT 1 cut(s) 1398
SmiMI CAYNNNNRTG 4 cut(s) 96, 642, 1033, 1116
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
SpeI ACTAGT 1 cut(s) 1748
SphI GCATGC 1 cut(s) 1502
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 4 cut(s) 506, 693, 823, 1204
SspI AATATT 2 cut(s) 138, 1851
SspMI CTAG 5 cut(s) 1076, 1316, 1326, 1460, 1749
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 7 cut(s) 371, 390, 1153, 1273, 1356, 1468, 1857
StyI CCWWGG 1 cut(s) 1325
SwaI ATTTAAAT 1 cut(s) 1398
TaaI ACNGT 4 cut(s) 932, 1337, 1995, 2020
TaiI ACGT 4 cut(s) 116, 345, 547, 1352
TaqI TCGA 2 cut(s) 318, 1648
TatI WGTACW 1 cut(s) 1898
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 3 cut(s) 335, 713, 1064
Tru1I TTAA 5 cut(s) 140, 366, 1397, 1586, 1695
Tru9I TTAA 5 cut(s) 140, 366, 1397, 1586, 1695
TscAI CASTG 2 cut(s) 1066, 1342
TseFI GTSAC 3 cut(s) 223, 1040, 1109
TseI GCWGC 2 cut(s) 690, 995
Tsp45I GTSAC 3 cut(s) 223, 1040, 1109
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 2 cut(s) 1066, 1342
Tth111I GACNNNGTC 1 cut(s) 1712
Van91I CCANNNNNTGG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 649
VpaK11BI GGWCC 3 cut(s) 388, 1383, 1708
XagI CCTNNNNNAGG 1 cut(s) 1473
XapI RAATTY 6 cut(s) 172, 920, 1220, 1393, 1555, 1772
XceI RCATGY 5 cut(s) 41, 84, 455, 1502, 1737
XmaJI CCTAGG 1 cut(s) 1325
XmiI GTMKAC 1 cut(s) 105
XmnI GAANNNNTTC 2 cut(s) 155, 1644
XspI CTAG 5 cut(s) 1076, 1316, 1326, 1460, 1749
Zsp2I ATGCAT 2 cut(s) 82, 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.