Rroxscaffold_4G00310940

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002244
Physical Location & Seq
Forward (+)
33096431 .. 33108947
12517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310940.1

Sequence Viewer

Length: 4113 bp
ATGGCTCCTAGAAAGAAACCCAATTCTTCTTCTTCTGCCGCTTCGTCTCGGAAATCGAACTCGAAAGCTCAGCCTCAGCCTTCCAAGTTCAGCATCCAACATTTCTTTGACCGCCACACCCAGAACTCTCAAAACCCCCAAACCACCACCTCTTCCACTCCTCACAACCCTAAGCCCCCAATCCCCGACTCCGCCACCGCCATTGTTCCGCAGAAGTCGATTCCGGAGATCCAAAACCCTGCCCCGATTGAAGCAACTAGTGCACTAGTAGCTTCTGGAAATCCTGAGAATCGGCGTGATTCGCAGTCGAGTGATAAGAACAGTACGCAGGTGACACCAACTGAGAATTTGGTGGCAGTGAGGTTTATTGATGCAGAGACGAATGCGGATGAGGAGGTCTCACCGGAGGTTTCGAAGTCTAAGTCTGTCAAGCGATTCAAGTTCTCCCCTGGAATGTTGATAAAGCAGAGTCAAGATGACGGAGGTGATGAGGTGACGTGGAGGACGTCTCCGGTCAATGAAAGACTTAAAGCAGTTTCAAAGCACAATCCTGAGATGATGAGAGTGTTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGCAAAACAAGGCATCTGGAAAGGTTGAGAAATCCCTGTCGTCGGTGTCCCTGAATAGGGTTGGCGTGAAGAGGGTTAAACCGGATCACGATGTGGAACATAATGGAAATGTGGTCGATAGGATTATTTCTGAAATAGCTGGTAGTCAGAGTCCATTCCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTGCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATTTTTGTTGAAGATTCAGTACCTGATGATGTGGAGGCATCTCAATTACAAGTTAAAGATAAATCAGAGCATTTCGTAAAAAGAGTGGTGACAGCTCCAACAGAACAGGTTACTCGGGCATCTTCCAATTACAGTTTCCTTGTATTGGAGGTATCTGAGAATTGTAGACCTGATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTACGGGAAGAGTGGTTTTACAGTGTCATTGCACCTGGAGATACTGTCAACGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATAATCACCATAACTTTTTAATTGTTCACCCTGATGTCTTAGTGTCTGGAACTCGGGTTGCATCTAATTTCATTTGTCCTAGGCGAACTGTGCTGGATGAAAGGCTAAAAGGCAATGAGTATTCAACTCCAGCACTAATTGGCACAATATTGCATCAAGTTTTTGAGGCTGCACTTATGAAGGAGAAACCTACAATTACTTTCTTGGAGGAACAAGCAAGAATGGGGCTCCAAAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTTAACATCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAAGTCGCGATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTCGAAGCTAACAAAAATGAATCTGAAGTGAAGGTTATACCTTTGGAGTTTAAAACCGGGAAAGTGCCGAAGGACCAGGCAAGACTATTCTCTGAGCATAGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAAAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACAGAATCCAAGCTTGTGCAGAAGTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGGCACATGGAGGCAACACAGATAGCAGTGGATTGGGAGAATTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGATTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGCTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGTGATCATTCCACAAGTTGCCTTTCTTCTGTGGTTCTTGATAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTACTGGTTTGTCTTCTCTCAATGTGGGGACATCCAATGGAGATTTTCAGAAGGCTGCTTCTTCACCCTCAAAATATGCAGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTAGCTGTTGCAAGGGGGGTTATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGAGCAATCCTTCTTCAGAGGCAAGTGATCTCCATCGGGAGCTCTGGCGGGTTGACAAAGATGAATTTATGACATCATTTGCCACAATGAGGTTCAACCTTCTTCAACTTTTTCTTCCAAGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGGTTTGACAGTGGATGTATTACGTTCAGCCAGGATCCAGCAATCTCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTCGTGCCATTCTAAAGATACTTACAGCGAAGGATTATGCTCTTATACTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGAGAGGTGCATCAATTTTGCTTACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTACGCATTGGAAGACAGGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCTTTTCAGTGATAGTTTTGACAGCAATGAACATACAAAGTGTGGAAGACGTTAAGTCAAGATTGGATCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAATAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTTGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCCCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCGCATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGTATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGACAGATTGCGTTGTGGATCTCCTGAGATAGCCAATGCCAAACTCAGCTTTTCAAGTTTGAAGTCTCATTCATTGTGGATAAAGGAGGTTTTAAATCCAAGCAAACCAGTTATATTTATTAATACAGATGCTTTACCTGCCTTCGAGGCAAAGGACCACAAGATTGTGAATAACCCGATGGAAGCTCACATAATTGTAGAGGTTGTGGAGGAATTAGTTAACAGTGGAATTGAAGGGGATGATATTGGCATCATTACCCCGTATAACTCCCAGGCAGAGATCATTCGGCTTGCCATCAACCTTACTTCTGTGGAGATACATACCATTGACAAATACCAGGGAAGAGATAAAGATTGCATACTAGTCTCCTTTGTTAGGTCAACTGAAAATCCACGAAACTGCTCGTCCTCTCTGCTCGGAGACTGGCATAGGATTAATGTGGCTCTAACTCGTGCCAAGAAAAAGTTGATAATGGTCGGGTCACGCGAAACCCTATCCAAAGTTCTGCTGATGAAGCTTCTTATCGAGAAAGTTGATGAACAATCAGGCATCTTAAACGTGTCCAAGAAAGACATCAACTTTAAGAGGGAGCTCAAGAGATGTTCCCAGGTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1370

Amino Acids

152.08

Weight (kDa)

6.99

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 377 - 579 2.8e-64 DNA replication factor Dna2
AAA_11 PF13086 945 - 1043 7.4e-17 AAA domain
AAA_30 PF13604 946 - 1114 5.2e-09 AAA domain
AAA_19 PF13245 950 - 1114 1.1e-06 AAA domain
AAA_11 PF13086 1050 - 1118 6e-16 AAA domain
AAA_12 PF13087 1127 - 1327 1.5e-53 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2537
AarI CACCTGC 1 cut(s) 319
AatII GACGTC 1 cut(s) 509
Acc36I ACCTGC 2 cut(s) 319, 3640
Acc65I GGTACC 1 cut(s) 1881
AccB1I GGYRCC 1 cut(s) 1881
AccB7I CCANNNNNTGG 1 cut(s) 1700
AccI GTMKAC 2 cut(s) 1072, 1530
AccII CGCG 2 cut(s) 1652, 3981
AccIII TCCGGA 1 cut(s) 223
AclI AACGTT 2 cut(s) 1209, 1538
AclWI GGATC 8 cut(s) 223, 705, 1633, 2795, 2808, 3171, 3206, 3520
AcoI YGGCCR 1 cut(s) 2264
AcsI RAATTY 7 cut(s) 346, 1220, 2360, 2660, 2833, 2995, 3227
AcuI CTGAAG 3 cut(s) 1623, 1785, 2595
AcyI GRCGYC 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 7 cut(s) 325, 927, 1883, 2383, 3051, 3355, 3446
AfiI CCNNNNNNNGG 9 cut(s) 655, 669, 670, 1051, 1700, 2685, 2820, 2915, 3870
AflIII ACRYGT 1 cut(s) 4053
AhdI GACNNNNNGTC 2 cut(s) 2027, 3151
AhlI ACTAGT 4 cut(s) 257, 265, 3203, 3856
AjiI CACGTC 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 1025, 1057
Alw21I GWGCWC 5 cut(s) 265, 1096, 2093, 2640, 4089
Alw26I GTCTC 7 cut(s) 51, 371, 403, 513, 3564, 3865, 3909
Alw44I GTGCAC 2 cut(s) 261, 2089
AlwI GGATC 8 cut(s) 223, 705, 1633, 2795, 2808, 3171, 3206, 3520
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 3 cut(s) 1020, 1302, 3363
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 5 cut(s) 857, 2013, 2264, 3284, 3360
ApaLI GTGCAC 2 cut(s) 261, 2089
ApeKI GCWGC 2 cut(s) 1418, 2435
ApoI RAATTY 7 cut(s) 346, 1220, 2360, 2660, 2833, 2995, 3227
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AseI ATTAAT 2 cut(s) 3615, 3930
Asp700I GAANNNNTTC 1 cut(s) 3084
Asp718I GGTACC 1 cut(s) 1881
AspA2I CCTAGG 2 cut(s) 1328, 2765
AspLEI GCGC 1 cut(s) 3412
AspS9I GGNCC 5 cut(s) 1813, 2823, 3284, 3361, 3649
AsuC2I CCSGG 1 cut(s) 1798
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 3 cut(s) 1020, 1302, 3363
AvaII GGWCC 3 cut(s) 1813, 2823, 3649
AvrII CCTAGG 2 cut(s) 1328, 2765
AxyI CCTNAGG 2 cut(s) 2195, 3417
BaeGI GKGCMC 4 cut(s) 265, 1846, 2093, 2722
BamHI GGATCC 1 cut(s) 2800
BanI GGYRCC 1 cut(s) 1881
BanII GRGCYC 4 cut(s) 1479, 1693, 2640, 4089
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BauI CACGAG 1 cut(s) 3945
BbsI GAAGAC 4 cut(s) 1898, 2385, 3067, 3150
Bbv12I GWGCWC 5 cut(s) 265, 1096, 2093, 2640, 4089
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 2 cut(s) 1405, 2422
BccI CCATC 8 cut(s) 824, 1675, 2085, 2102, 2230, 2637, 3667, 3797
BcgI CGANNNNNNTGC 2 cut(s) 3078, 3112
BclI TGATCA 4 cut(s) 1927, 2284, 2845, 3322
BcnI CCSGG 1 cut(s) 1798
BcoDI GTCTC 7 cut(s) 51, 371, 403, 513, 3564, 3865, 3909
BcuI ACTAGT 4 cut(s) 257, 265, 3203, 3856
BfuAI ACCTGC 2 cut(s) 319, 3640
BglI GCCNNNNNGGC 1 cut(s) 3641
BglII AGATCT 1 cut(s) 1955
BisI GCNGC 5 cut(s) 39, 860, 1419, 2264, 2436
BlnI CCTAGG 2 cut(s) 1328, 2765
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 5 cut(s) 40, 861, 1420, 2265, 2437
Bme18I GGWCC 3 cut(s) 1813, 2823, 3649
BmeRI GACNNNNNGTC 2 cut(s) 2027, 3151
BmeT110I CYCGRG 3 cut(s) 1020, 1302, 3363
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 5 cut(s) 1813, 2823, 3284, 3361, 3649
BmiI GGNNCC 7 cut(s) 6, 1478, 1692, 1883, 2763, 2802, 2825
BpiI GAAGAC 4 cut(s) 1898, 2385, 3067, 3150
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 493, 525
BpmI CTGGAG 3 cut(s) 1215, 1362, 1904
Bpu10I CCTNAGC 2 cut(s) 75, 171
Bpu1102I GCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 2002, 4073
BpuMI CCSGG 1 cut(s) 1798
BsaBI GATNNNNATC 2 cut(s) 2628, 3327
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 403
BsaWI WCCGGW 4 cut(s) 223, 403, 511, 694
BsaXI ACNNNNNCTCC 2 cut(s) 1188, 1218
Bsc4I CCNNNNNNNGG 9 cut(s) 655, 669, 670, 1051, 1700, 2685, 2820, 2915, 3870
Bse1I ACTGG 7 cut(s) 1906, 1921, 2389, 3272, 3442, 3602, 3923
Bse21I CCTNAGG 2 cut(s) 2195, 3417
Bse3DI GCAATG 6 cut(s) 872, 1185, 1369, 3094, 3129, 3431
Bse8I GATNNNNATC 2 cut(s) 2628, 3327
BseAI TCCGGA 1 cut(s) 223
BseGI GGATG 8 cut(s) 93, 394, 1351, 2411, 2786, 3253, 3412, 3739
BseJI GATNNNNATC 2 cut(s) 2628, 3327
BseLI CCNNNNNNNGG 9 cut(s) 655, 669, 670, 1051, 1700, 2685, 2820, 2915, 3870
BseMI GCAATG 6 cut(s) 872, 1185, 1369, 3094, 3129, 3431
BseNI ACTGG 7 cut(s) 1906, 1921, 2389, 3272, 3442, 3602, 3923
BseRI GAGGAG 4 cut(s) 150, 407, 1691, 3330
BseSI GKGCMC 4 cut(s) 265, 1846, 2093, 2722
BseXI GCAGC 2 cut(s) 1405, 2422
BsgI GTGCAG 2 cut(s) 1404, 2083
Bsh1236I CGCG 2 cut(s) 1652, 3981
BshFI GGCC 5 cut(s) 859, 2015, 2266, 3286, 3362
BshNI GGYRCC 1 cut(s) 1881
BsiHKAI GWGCWC 5 cut(s) 265, 1096, 2093, 2640, 4089
BsiHKCI CYCGRG 3 cut(s) 1020, 1302, 3363
BsiSI CCGG 5 cut(s) 224, 404, 512, 695, 1797
BslFI GGGAC 5 cut(s) 646, 1538, 2422, 2809, 3192
BslI CCNNNNNNNGG 9 cut(s) 655, 669, 670, 1051, 1700, 2685, 2820, 2915, 3870
BsmAI GTCTC 7 cut(s) 51, 371, 403, 513, 3564, 3865, 3909
BsmBI CGTCTC 3 cut(s) 51, 371, 513
BsmFI GGGAC 5 cut(s) 646, 1538, 2422, 2809, 3192
BsmI GAATGC 2 cut(s) 388, 2910
BsnI GGCC 5 cut(s) 859, 2015, 2266, 3286, 3362
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 3 cut(s) 1020, 1302, 3363
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 9 cut(s) 265, 1096, 1479, 1693, 1846, 2093, 2640, 2722, 4089
Bsp13I TCCGGA 1 cut(s) 223
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 1652
BspANI GGCC 5 cut(s) 859, 2015, 2266, 3286, 3362
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 2 cut(s) 1652, 3981
BspHI TCATGA 2 cut(s) 799, 1516
BspLI GGNNCC 7 cut(s) 6, 1478, 1692, 1883, 2763, 2802, 2825
BspMI ACCTGC 2 cut(s) 319, 3640
BspPI GGATC 8 cut(s) 223, 705, 1633, 2795, 2808, 3171, 3206, 3520
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 1881
BspTNI GGTCTC 1 cut(s) 403
BsrDI GCAATG 6 cut(s) 872, 1185, 1369, 3094, 3129, 3431
BsrI ACTGG 7 cut(s) 1906, 1921, 2389, 3272, 3442, 3602, 3923
BssNAI GTATAC 1 cut(s) 1531
BssNI GRCGYC 1 cut(s) 506
BssSI CACGAG 1 cut(s) 3945
BssT1I CCWWGG 3 cut(s) 1231, 1328, 2765
Bst1107I GTATAC 1 cut(s) 1531
Bst2BI CACGAG 1 cut(s) 3945
Bst6I CTCTTC 8 cut(s) 157, 677, 1137, 1161, 1869, 2273, 3344, 3832
BstACI GRCGYC 1 cut(s) 506
BstAPI GCANNNNNTGC 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 6 cut(s) 614, 810, 2940, 3216, 3398, 3786
BstEII GGTNACC 1 cut(s) 2480
BstENI CCTNNNNNAGG 2 cut(s) 2913, 3868
BstF5I GGATG 8 cut(s) 93, 394, 1351, 2411, 2786, 3253, 3412, 3739
BstFNI CGCG 2 cut(s) 1652, 3981
BstHHI GCGC 1 cut(s) 3412
BstMAI GTCTC 7 cut(s) 51, 371, 403, 513, 3564, 3865, 3909
BstNSI RCATGY 5 cut(s) 1105, 1509, 1895, 2942, 3192
BstPI GGTNACC 1 cut(s) 2480
BstSLI GKGCMC 4 cut(s) 265, 1846, 2093, 2722
BstUI CGCG 2 cut(s) 1652, 3981
BstV1I GCAGC 2 cut(s) 1405, 2422
BstV2I GAAGAC 4 cut(s) 1898, 2385, 3067, 3150
BstX2I RGATCY 5 cut(s) 228, 1625, 1955, 2800, 3512
BstYI RGATCY 5 cut(s) 228, 1625, 1955, 2800, 3512
BstZ17I GTATAC 1 cut(s) 1531
Bsu36I CCTNAGG 2 cut(s) 2195, 3417
BsuRI GGCC 5 cut(s) 859, 2015, 2266, 3286, 3362
BtrI CACGTC 1 cut(s) 498
BtsCI GGATG 8 cut(s) 93, 394, 1351, 2411, 2786, 3253, 3412, 3739
BtsI GCAGTG 3 cut(s) 363, 2137, 2506
BtsIMutI CAGTG 9 cut(s) 363, 1103, 1186, 1244, 2137, 2506, 2782, 3111, 3724
BtuMI TCGCGA 1 cut(s) 1652
BveI ACCTGC 2 cut(s) 319, 3640
Cac8I GCNNGC 6 cut(s) 614, 810, 2940, 3216, 3398, 3786
CaiI CAGNNNCTG 1 cut(s) 929
CciI TCATGA 2 cut(s) 799, 1516
CfoI GCGC 1 cut(s) 3412
Cfr13I GGNCC 5 cut(s) 1813, 2823, 3284, 3361, 3649
CseI GACGC 1 cut(s) 2840
Csp6I GTAC 7 cut(s) 324, 926, 1882, 2382, 3050, 3354, 3445
CspCI CAANNNNNGTGG 4 cut(s) 184, 219, 2256, 2291
CviQI GTAC 7 cut(s) 324, 926, 1882, 2382, 3050, 3354, 3445
DraI TTTAAA 3 cut(s) 1792, 2838, 3588
DraIII CACNNNGTG 1 cut(s) 706
DriI GACNNNNNGTC 2 cut(s) 2027, 3151
EaeI YGGCCR 1 cut(s) 2264
Eam1104I CTCTTC 8 cut(s) 157, 677, 1137, 1161, 1869, 2273, 3344, 3832
Eam1105I GACNNNNNGTC 2 cut(s) 2027, 3151
EarI CTCTTC 8 cut(s) 157, 677, 1137, 1161, 1869, 2273, 3344, 3832
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 2 cut(s) 2638, 4087
Eco130I CCWWGG 3 cut(s) 1231, 1328, 2765
Eco147I AGGCCT 1 cut(s) 2015
Eco24I GRGCYC 4 cut(s) 1479, 1693, 2640, 4089
Eco31I GGTCTC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 1656
Eco47I GGWCC 3 cut(s) 1813, 2823, 3649
Eco53kI GAGCTC 2 cut(s) 2638, 4087
Eco57I CTGAAG 3 cut(s) 1623, 1785, 2595
Eco81I CCTNAGG 2 cut(s) 2195, 3417
Eco88I CYCGRG 3 cut(s) 1020, 1302, 3363
Eco91I GGTNACC 1 cut(s) 2480
EcoICRI GAGCTC 2 cut(s) 2638, 4087
EcoNI CCTNNNNNAGG 2 cut(s) 2913, 3868
EcoO65I GGTNACC 1 cut(s) 2480
EcoRV GATATC 1 cut(s) 1656
EcoT14I CCWWGG 3 cut(s) 1231, 1328, 2765
EcoT22I ATGCAT 2 cut(s) 1507, 3242
EcoT38I GRGCYC 4 cut(s) 1479, 1693, 2640, 4089
ErhI CCWWGG 3 cut(s) 1231, 1328, 2765
Esp3I CGTCTC 3 cut(s) 51, 371, 513
FaqI GGGAC 5 cut(s) 646, 1538, 2422, 2809, 3192
FauI CCCGC 1 cut(s) 2637
FbaI TGATCA 4 cut(s) 1927, 2284, 2845, 3322
FblI GTMKAC 2 cut(s) 1072, 1530
Fnu4HI GCNGC 5 cut(s) 39, 860, 1419, 2264, 2436
FokI GGATG 8 cut(s) 80, 401, 1358, 2398, 2793, 3260, 3399, 3746
FriOI GRGCYC 4 cut(s) 1479, 1693, 2640, 4089
Fsp4HI GCNGC 5 cut(s) 39, 860, 1419, 2264, 2436
GlaI GCGC 1 cut(s) 3411
GluI GCNGC 5 cut(s) 39, 860, 1419, 2264, 2436
GsuI CTGGAG 3 cut(s) 1215, 1362, 1904
HaeIII GGCC 5 cut(s) 859, 2015, 2266, 3286, 3362
HapII CCGG 5 cut(s) 224, 404, 512, 695, 1797
HgaI GACGC 1 cut(s) 2840
HhaI GCGC 1 cut(s) 3412
Hin1I GRCGYC 1 cut(s) 506
Hin6I GCGC 1 cut(s) 3410
HinP1I GCGC 1 cut(s) 3410
HincII GTYRAC 6 cut(s) 1207, 1576, 2650, 2752, 3715, 3876
HindII GTYRAC 6 cut(s) 1207, 1576, 2650, 2752, 3715, 3876
HindIII AAGCTT 5 cut(s) 806, 1605, 2056, 3385, 4010
HpaI GTTAAC 2 cut(s) 1576, 3715
HpaII CCGG 5 cut(s) 224, 404, 512, 695, 1797
Hpy99I CGWCG 2 cut(s) 658, 2856
HpyCH4IV ACGT 8 cut(s) 497, 506, 1209, 1538, 1984, 2789, 3147, 4053
HpySE526I ACGT 8 cut(s) 497, 506, 1209, 1538, 1984, 2789, 3147, 4053
Hsp92I GRCGYC 1 cut(s) 506
HspAI GCGC 1 cut(s) 3410
Kpn2I TCCGGA 1 cut(s) 223
KpnI GGTACC 1 cut(s) 1885
Ksp22I TGATCA 4 cut(s) 1927, 2284, 2845, 3322
KspAI GTTAAC 2 cut(s) 1576, 3715
LmnI GCTCC 9 cut(s) 10, 1006, 1482, 1696, 2239, 2598, 2635, 2767, 4084
Lsp1109I GCAGC 2 cut(s) 1405, 2422
MaeII ACGT 8 cut(s) 497, 506, 1209, 1538, 1984, 2789, 3147, 4053
MflI RGATCY 5 cut(s) 228, 1625, 1955, 2800, 3512
MhlI GDGCHC 9 cut(s) 265, 1096, 1479, 1693, 1846, 2093, 2640, 2722, 4089
MlyI GAGTC 3 cut(s) 182, 478, 772
MmeI TCCRAC 3 cut(s) 121, 1028, 1854
Mph1103I ATGCAT 2 cut(s) 1507, 3242
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 3084
MslI CAYNNNNRTG 5 cut(s) 1281, 1521, 2082, 2473, 2556
MspI CCGG 5 cut(s) 224, 404, 512, 695, 1797
Mva1269I GAATGC 2 cut(s) 388, 2910
MvnI CGCG 2 cut(s) 1652, 3981
NciI CCSGG 1 cut(s) 1798
NlaIV GGNNCC 7 cut(s) 6, 1478, 1692, 1883, 2763, 2802, 2825
NmuCI GTSAC 7 cut(s) 331, 493, 994, 2480, 2549, 3494, 3975
NruI TCGCGA 1 cut(s) 1652
NsiI ATGCAT 2 cut(s) 1507, 3242
NspI RCATGY 5 cut(s) 1105, 1509, 1895, 2942, 3192
NspV TTCGAA 1 cut(s) 413
PaeI GCATGC 1 cut(s) 2942
PagI TCATGA 2 cut(s) 799, 1516
PaqCI CACCTGC 1 cut(s) 319
PceI AGGCCT 1 cut(s) 2015
PcsI WCGNNNNNNNCGW 2 cut(s) 503, 3978
PctI GAATGC 2 cut(s) 388, 2910
PdmI GAANNNNTTC 1 cut(s) 3084
PflFI GACNNNGTC 1 cut(s) 1239
PflMI CCANNNNNTGG 1 cut(s) 1700
PkrI GCNGC 5 cut(s) 40, 861, 1420, 2265, 2437
PleI GAGTC 3 cut(s) 182, 477, 771
PpsI GAGTC 3 cut(s) 182, 477, 771
PshBI ATTAAT 2 cut(s) 3615, 3930
PsiI TTATAA 1 cut(s) 2537
Psp124BI GAGCTC 2 cut(s) 2640, 4089
Psp1406I AACGTT 2 cut(s) 1209, 1538
PspEI GGTNACC 1 cut(s) 2480
PspN4I GGNNCC 7 cut(s) 6, 1478, 1692, 1883, 2763, 2802, 2825
PspPI GGNCC 5 cut(s) 1813, 2823, 3284, 3361, 3649
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 5 cut(s) 228, 1625, 1955, 2800, 3512
PsyI GACNNNGTC 1 cut(s) 1239
RruI TCGCGA 1 cut(s) 1652
RsaI GTAC 7 cut(s) 325, 927, 1883, 2383, 3051, 3355, 3446
RsaNI GTAC 7 cut(s) 324, 926, 1882, 2382, 3050, 3354, 3445
RseI CAYNNNNRTG 5 cut(s) 1281, 1521, 2082, 2473, 2556
SacI GAGCTC 2 cut(s) 2640, 4089
SatI GCNGC 5 cut(s) 39, 860, 1419, 2264, 2436
Sau96I GGNCC 5 cut(s) 1813, 2823, 3284, 3361, 3649
SchI GAGTC 3 cut(s) 182, 478, 772
SduI GDGCHC 9 cut(s) 265, 1096, 1479, 1693, 1846, 2093, 2640, 2722, 4089
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 3 cut(s) 1813, 2823, 3649
SmiI ATTTAAAT 1 cut(s) 2838
SmiMI CAYNNNNRTG 5 cut(s) 1281, 1521, 2082, 2473, 2556
SmlI CTYRAG 2 cut(s) 2017, 4088
SmoI CTYRAG 2 cut(s) 2017, 4088
SpeI ACTAGT 4 cut(s) 257, 265, 3203, 3856
SphI GCATGC 1 cut(s) 2942
SseBI AGGCCT 1 cut(s) 2015
SspI AATATT 3 cut(s) 1398, 1563, 3306
SstI GAGCTC 2 cut(s) 2640, 4089
StuI AGGCCT 1 cut(s) 2015
StyI CCWWGG 3 cut(s) 1231, 1328, 2765
SwaI ATTTAAAT 1 cut(s) 2838
TaiI ACGT 8 cut(s) 500, 509, 1212, 1541, 1987, 2792, 3150, 4056
TatI WGTACW 1 cut(s) 3353
TauI GCSGC 3 cut(s) 41, 862, 2266
TscAI CASTG 9 cut(s) 363, 1103, 1186, 1244, 2137, 2506, 2782, 3111, 3724
TseFI GTSAC 7 cut(s) 331, 493, 994, 2480, 2549, 3494, 3975
TseI GCWGC 2 cut(s) 1418, 2435
Tsp45I GTSAC 7 cut(s) 331, 493, 994, 2480, 2549, 3494, 3975
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 9 cut(s) 363, 1103, 1186, 1244, 2137, 2506, 2782, 3111, 3724
Tth111I GACNNNGTC 1 cut(s) 1239
Van91I CCANNNNNTGG 1 cut(s) 1700
VneI GTGCAC 2 cut(s) 261, 2089
VpaK11BI GGWCC 3 cut(s) 1813, 2823, 3649
VspI ATTAAT 2 cut(s) 3615, 3930
XagI CCTNNNNNAGG 2 cut(s) 2913, 3868
XapI RAATTY 7 cut(s) 346, 1220, 2360, 2660, 2833, 2995, 3227
XceI RCATGY 5 cut(s) 1105, 1509, 1895, 2942, 3192
XmaJI CCTAGG 2 cut(s) 1328, 2765
XmiI GTMKAC 2 cut(s) 1072, 1530
XmnI GAANNNNTTC 1 cut(s) 3084
ZraI GACGTC 1 cut(s) 507
Zsp2I ATGCAT 2 cut(s) 1507, 3242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.