MD07G1085900.v1.1

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002244
Physical Location & Seq
Reverse (-)
8897688 .. 8910239
12552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1085900.v1.1.491

Sequence Viewer

Length: 4128 bp
ATGGCTCCGAGAAAGAAACCCATTTCTTCCAACACGACGTCGTCTAAGAAATCCAACTCGAAAGCTCAGCCGCAGCCCTCCAAGTTCGGCATCCAACATTTTTTTGAGCGCCACACCCAGAACTCCCAGAAACCCAAAGAGGTCGCCGCTTCTTCCCCTCCTCAGAACCCTAAAACCCTAGCCGCCACCGTCTCCCCCACCGTTGCTCCAGTACAATCGGTTCCAAAGATCCGAAAGCCTGCTCAGATTGATGCGGGTAATGCGGTTTTAGCTTTGGTGAATGATGAAATTCAGTCGATTTCGCAATTGGGGAATAAGAATAGCATGTCGAAGTACACGCCGGAGAATTTGATGGCGGCTGGAGTTGGTGGTGATAAGGAGAATGTTGACGAGGAGGCCTCGCCGGAGATTTCAAAGTCTAAGTCTTTAAAGCGCTTCAAGTTTTCACCTGGAATGTTGATTAAGCAGAGCCAAGATGACGGAGGCGATGAGGTAACATGGAGGATATCTCCGGTAAATGAAAGACTCCAAGCTGTTTCAAAACGCATGCCGGAGATTATTAGAGTGTTGGCTGACTCTTCAAGGCTTAAATCTTTAAGCATTAGTCAGTGCCCGCAAAACAAGACTTCTCCTGTTAAAGCTGTAACGGTTGAGAAGTGGATTCCCTCACGGACACCGAATACTTCTGATAGATCGTTGGTATCCCTGAATAGGGTTGGTGTAAAAAGAGTTAAACCAGACCAGGATATGGATAGTAATGGAGCTTTTTCAGGAATAGATAGTAGGCAGAGTCCATTCCGAACTCCACCTTCTCTACCGTACTGTCATGATAAGCTTGCTAATGCATGTAATGGAGTTTCAGATCAGCCGGGGTTGAGGCAGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTGGAAGATGTTATTTCTGTTGAAGATTCAGTGTCTAGTGATGTGGAGGAATCTCCATTTCAAGTTGAAGATAAAAAAAGCAAGAGAATGTCTATCAGTTTGGATCACACAGTTAAAAGAGTAGAGACAGCTCTAACAGGAAAGGTTCTTCGGAAATCTTCATATTGTAGTTTTCTTGTATTGGAGGTATCTGAGAAGTGTGGAGATGCTGATTCATCTGGTGATCAATGCCTGTTTAAGGTTATAAGGTTACTAAATGAGCAAAGTGGAGAAGAGCGCTCTGTATATTTACGAGATGAGTGGTTGTACAGTGTCATTGCTCCTGGAGATACTGTCAATGTTATTGGCGAATTTGATAACCAAGGACAGTGTCATGTGGATCGTCACAATAATTTTATAATTGTTCATCCAGATGTTTTGGTGTCTGGAACTAGGGTTACAGCTAGCTTCATTTGCCCTAGGCGAACTGTCCTGGATGAAAGACTAAAATCCAATGAGCATTCAACTGCAGCACTAAGTGGTACAATATTGCATCGAGTTTTTGAGGCTGGACTTATGAAGGAGATACCTACAGTAACTTTCTTGGAGGAACGCACAAGAACGGTGCTCCAGAAAAACCTTGAGAACTTGTATGCTTGTGGAGTGAACGAAAATGATATGTACAAAACATTGATCGAGGCTGTCCCCAAAATACTGAATTGGGTTAACCTCTTCAAAAATTCCCAGGGTTCAAAAGCTCCTTCTGTTGATTTTGGATCTGACATTGGGGTGAAAAAGGTAATTGATATTGAGGAGATGGCCTGGGCTCCAAAATATGGTCTGAAAGGCCAGATTGATGCTTCTGTCAGAGTGGAAGTTGAATCTAACAGTGGTGAATCTCATGAAAAGGTCATGCCTTTAGAGTTCAAAAGTGGGAAAGTACCAAAGGGCCAGTCGTCTATGGAGCATAGTGCCCAAGTGATCTTATACACTCTCCTCATGTCGGAGAGGTACCAGAAGCATGTCGATACTGGTCTCCTATGTTATCTGCAGTCAGATCAGACACAGGGGATTGTTGCTAGAAGATCTGACCTAGTTGGTCTAATCATGCGACGTAATGAACTTGCAAATGATATCCTCAAGGCATCACAAACACAAGTACTGCCCCCAATGTTACAGTGTCAGAGCATTTGTGGAAGTTGCCGCCATCTAAATGTTTGTACTGTCTATCACAAGGTACATGGTGGAAGCACAGAGAGCAGCGGATTGGCAGATTTGTTTGATTCAAATACTCAGTACCTAACAAATGCTCATGGTGTTTTCTTAAGGCTGTGGGATGGGTTGATTGATTTGGAAGCTAAAGAGATGCAGCTTGTAAAGAATGAAATTTGGCGGTCACATAGCTCCAATAGTGATTATGCCACTGGTTGCCTTTCTTTTATTGTTCTTGATGGTGATTCTCCATGTTCAGCATCCGGAAAAGATAGCAGATTTGTTTATCGTTTTTTGCGTAACAGTTTTCCTTCTCTCAATGGGAGGACATCTGATATAGATTTTTCAAGCGCCAGTTCTTCAAGAAAAGATATAGATTGCACACTAAAATGTGGTGACTTTGTGATACTTAGCAGCGAATCTGGTAGTCTACCTGTTGCAAGCGGAATCATAATGGATATAAGTCAATCCCATGTTTCTGTTTCTTTCTCTAAGCACTTACGACTTCCTTGGAGCAATCCTTCAGAGGCAAGTGATCTCCTTCAGGAGGTCTGGCGTGTTGACAAAGATGATTTCGTGACATCATTTTCAGTAATGAGGTTTAACCTTGTTCAACTTTTTCTTCAAAGTACACAAGCTGCTCACCTTAGAAAGATGATTGTTGACCTAGAAGCTCCTAGGTTTGACAATGGGTCTCCACTCAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAGAGAAATTTAAATGATGATCAGCGTCGAGCCATTCTTAAGATACTTACAGCAAAGGACTACGCTCTTATACTAGGAATGCCTGGAACGGGCAAGACATCCACCATGGTGCATGCTGTGAAAGCTCTGTTGATTAGAGGTTCATCCATTTTGCTTACATCCTACACAAACTCCGCTGTTGATAATCTACTTGTCAAATTAAAGGCTGAGGATATTGATTTTATACGCATTGGAAGACATGAAGCTGTGCATGAAGAAATTCGAGGCCATTGCTTCTCAGAGATGAACATTCAAACTGTGGAAGGCATTAAGTTAATATTAGACCAAGTCAAGGTGGTTGCCGTTACATGCTTGGGGATCACCAGCCCCTTGCTTGCCAATAAGAGATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTGTCTCTTGGGCCTCTGATGTTTGCTTCAAAGTTTGTCCTGGTTGGCGATCATTATCAGTTACCTCCTCTGGTCAAGAGTACGGAGGCTCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGTAGGCTTTCAGAAGCGCATCCTCAGGCAATTTCAGCCTTGCAAAGCCAGTACCGTATGTGTCGAGGCATTATGGCACTGTCAAATGCCTTGATTTATGGTGATCGATTGCGATGTGGCTCTCCTGAAATAGAAAATGCCAAACTCAAGGTTTCAAGTTCAATACCTCATTCATTTTGGATACAGGAGGTTATAAACCCAAGCAAGCCAGTTATATTTATTAATACAGATTCTTTACCTGCTTTTGAGGCAAAGGATCACAAGATTGTGAGTAACCCGACGGAAGCTAACATAATTGTACAGGTTGTGGAGGGACTAGTCAAGAGTGGAATAAAGGGGGAAGATATTGGTGTCATTACCCCATATAACTCCCAGGCAGAGATCATCCGGCTTGCCCTCAGCCGCCTGACCTCTGTTGAGATACATACCATTGACAAATATCAGGGAAGAGACAAGGATTGTATACTAGTGTCCTTCGTTAGGTCAATGGAAAATCCCACGAATTGCTCCTCCTCGCTGCTTGGAGACTGGCATAGGATTAATGTAGCTCTCACTCGTGCCAAGAAAAAGTTGGTAATGGTTGGATCGTGCAAAACCCTATCGAATGTTGTGCTGTTGAAGCTTCTTATCGAGCAAGTTGAACAACAGTCGGGCATCTTAAATGTATCGAACAAGGATATTAAGTTTAAAAGAGAGTTAAAGAGATGCTCTCAGGCTCAGGCTCAGGCCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1376

Amino Acids

152.03

Weight (kDa)

8.38

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 392 - 589 7.2e-61 DNA replication factor Dna2
AAA_11 PF13086 950 - 1041 8.4e-16 AAA domain
AAA_30 PF13604 951 - 1114 3.6e-09 AAA domain
UvrD-helicase PF00580 951 - 1049 5.7e-07 UvrD/REP helicase N-terminal domain
AAA_19 PF13245 955 - 1115 2.6e-07 AAA domain
AAA_11 PF13086 1051 - 1118 7.9e-18 AAA domain
AAA_12 PF13087 1127 - 1328 2.9e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1160, 1313, 3587
AatII GACGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 3640
Acc65I GGTACC 1 cut(s) 1905
AccB1I GGYRCC 1 cut(s) 1905
AccB7I CCANNNNNTGG 2 cut(s) 748, 1730
AccI GTMKAC 2 cut(s) 2536, 3856
AccIII TCCGGA 1 cut(s) 2369
AclWI GGATC 9 cut(s) 223, 1026, 1302, 1678, 2810, 2823, 3206, 3657, 3985
AcsI RAATTY 7 cut(s) 288, 346, 1265, 1633, 2280, 2848, 3099
AcuI CTGAAG 2 cut(s) 2613, 2633
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 1433
AfeI AGCGCT 2 cut(s) 434, 1193
AflII CTTAAG 2 cut(s) 2218, 2879
AhdI GACNNNNNGTC 1 cut(s) 2797
AhlI ACTAGT 2 cut(s) 3709, 3859
AleI CACNNNNGTG 1 cut(s) 2948
Alw21I GWGCWC 1 cut(s) 1524
Alw26I GTCTC 7 cut(s) 196, 1034, 1934, 2805, 3282, 3837, 3912
AlwI GGATC 9 cut(s) 223, 1026, 1302, 1678, 2810, 2823, 3206, 3657, 3985
Ama87I CYCGRG 1 cut(s) 3363
Aor13HI TCCGGA 1 cut(s) 2369
Aor51HI AGCGCT 2 cut(s) 434, 1193
AoxI GGCC 7 cut(s) 396, 1713, 1741, 1843, 3106, 3284, 4119
ApeKI GCWGC 8 cut(s) 73, 880, 1424, 2154, 2263, 2520, 2744, 3910
ApoI RAATTY 7 cut(s) 288, 346, 1265, 1633, 2280, 2848, 3099
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 1832, 1864
AseI ATTAAT 2 cut(s) 3615, 3933
Asp700I GAANNNNTTC 1 cut(s) 3099
Asp718I GGTACC 1 cut(s) 1905
AspA2I CCTAGG 2 cut(s) 1373, 2783
AspLEI GCGC 5 cut(s) 111, 435, 1194, 2459, 3412
AspS9I GGNCC 3 cut(s) 1843, 2838, 3284
AsuC2I CCSGG 1 cut(s) 870
AsuNHI GCTAGC 1 cut(s) 1358
AvaI CYCGRG 1 cut(s) 3363
AvaII GGWCC 1 cut(s) 2838
AvrII CCTAGG 2 cut(s) 1373, 2783
AxyI CCTNAGG 1 cut(s) 3417
BaeGI GKGCMC 2 cut(s) 614, 1870
BamHI GGATCC 1 cut(s) 2815
BanI GGYRCC 1 cut(s) 1905
BanII GRGCYC 1 cut(s) 1723
BauI CACGAG 1 cut(s) 3948
BbsI GAAGAC 1 cut(s) 3082
Bbv12I GWGCWC 1 cut(s) 1524
BbvCI CCTCAGC 2 cut(s) 3048, 3791
BbvI GCAGC 8 cut(s) 85, 892, 1436, 2166, 2275, 2532, 2731, 3897
BccI CCATC 5 cut(s) 346, 1705, 2109, 2225, 2339
BceAI ACGGC 1 cut(s) 3167
BcgI CGANNNNNNTGC 4 cut(s) 3093, 3127, 3985, 4019
BciVI GTATCC 2 cut(s) 712, 3567
BclI TGATCA 2 cut(s) 1138, 2860
BcnI CCSGG 1 cut(s) 870
BcoDI GTCTC 7 cut(s) 196, 1034, 1934, 2805, 3282, 3837, 3912
BcuI ACTAGT 2 cut(s) 3709, 3859
BfmI CTRYAG 3 cut(s) 1422, 1485, 1943
BfoI RGCGCY 4 cut(s) 112, 436, 1195, 2460
BfrI CTTAAG 2 cut(s) 2218, 2879
BfuAI ACCTGC 1 cut(s) 3640
BfuI GTATCC 2 cut(s) 712, 3567
BglII AGATCT 1 cut(s) 1979
BlnI CCTAGG 2 cut(s) 1373, 2783
BlpI GCTNAGC 1 cut(s) 66
BmcAI AGTACT 1 cut(s) 2055
Bme18I GGWCC 1 cut(s) 2838
BmeRI GACNNNNNGTC 1 cut(s) 2797
BmeT110I CYCGRG 1 cut(s) 3363
BmgT120I GGNCC 3 cut(s) 1843, 2838, 3284
BmiI GGNNCC 6 cut(s) 6, 222, 1722, 1907, 2817, 2840
BmtI GCTAGC 1 cut(s) 1362
BpiI GAAGAC 1 cut(s) 3082
BplI GAGNNNNNCTC 6 cut(s) 383, 415, 493, 525, 4087, 4119
BpmI CTGGAG 4 cut(s) 192, 381, 1260, 1508
Bpu10I CCTNAGC 4 cut(s) 3048, 3791, 4110, 4116
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 1556, 2018, 3524
BpuMI CCSGG 1 cut(s) 870
Bsa29I ATCGAT 1 cut(s) 3499
BsaBI GATNNNNATC 1 cut(s) 3327
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 2 cut(s) 1934, 2805
BsaWI WCCGGW 2 cut(s) 511, 2369
BsaXI ACNNNNNCTCC 6 cut(s) 190, 220, 1233, 1263, 2996, 3026
Bse1I ACTGG 9 cut(s) 209, 1846, 1930, 2323, 2460, 3272, 3442, 3602, 3926
Bse21I CCTNAGG 1 cut(s) 3417
Bse3DI GCAATG 3 cut(s) 893, 1230, 3109
Bse8I GATNNNNATC 1 cut(s) 3327
BseAI TCCGGA 1 cut(s) 2369
BseCI ATCGAT 1 cut(s) 3499
BseJI GATNNNNATC 1 cut(s) 3327
BseMI GCAATG 3 cut(s) 893, 1230, 3109
BseNI ACTGG 9 cut(s) 209, 1846, 1930, 2323, 2460, 3272, 3442, 3602, 3926
BseRI GAGGAG 7 cut(s) 150, 407, 1721, 1880, 3330, 3892, 3895
BseSI GKGCMC 2 cut(s) 614, 1870
BseXI GCAGC 8 cut(s) 85, 892, 1436, 2166, 2275, 2532, 2731, 3897
BshFI GGCC 7 cut(s) 398, 1715, 1743, 1845, 3108, 3286, 4121
BshNI GGYRCC 1 cut(s) 1905
BshVI ATCGAT 1 cut(s) 3499
BsiHKAI GWGCWC 1 cut(s) 1524
BsiHKCI CYCGRG 1 cut(s) 3363
BsiSI CCGG 7 cut(s) 341, 404, 512, 551, 869, 2370, 3781
BslFI GGGAC 3 cut(s) 1583, 2824, 3720
BsmAI GTCTC 7 cut(s) 196, 1034, 1934, 2805, 3282, 3837, 3912
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 1583, 2824, 3720
BsmI GAATGC 2 cut(s) 1414, 2925
BsnI GGCC 7 cut(s) 398, 1715, 1743, 1845, 3108, 3286, 4121
Bso31I GGTCTC 2 cut(s) 1934, 2805
BsoBI CYCGRG 1 cut(s) 3363
Bsp1286I GDGCHC 4 cut(s) 614, 1524, 1723, 1870
Bsp13I TCCGGA 1 cut(s) 2369
Bsp1407I TGTACA 3 cut(s) 1221, 1575, 3691
Bsp1720I GCTNAGC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 2946
BspANI GGCC 7 cut(s) 398, 1715, 1743, 1845, 3108, 3286, 4121
BspDI ATCGAT 1 cut(s) 3499
BspEI TCCGGA 1 cut(s) 2369
BspHI TCATGA 2 cut(s) 826, 1795
BspLI GGNNCC 6 cut(s) 6, 222, 1722, 1907, 2817, 2840
BspMAI CTGCAG 2 cut(s) 1426, 1947
BspMI ACCTGC 1 cut(s) 3640
BspOI GCTAGC 1 cut(s) 1362
BspPI GGATC 9 cut(s) 223, 1026, 1302, 1678, 2810, 2823, 3206, 3657, 3985
BspQI GCTCTTC 1 cut(s) 1182
BspT107I GGYRCC 1 cut(s) 1905
BspTI CTTAAG 2 cut(s) 2218, 2879
BspTNI GGTCTC 2 cut(s) 1934, 2805
BsrDI GCAATG 3 cut(s) 893, 1230, 3109
BsrGI TGTACA 3 cut(s) 1221, 1575, 3691
BsrI ACTGG 9 cut(s) 209, 1846, 1930, 2323, 2460, 3272, 3442, 3602, 3926
BssNAI GTATAC 1 cut(s) 3857
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 3948
BssT1I CCWWGG 5 cut(s) 1276, 1373, 2615, 2783, 2946
Bst1107I GTATAC 1 cut(s) 3857
Bst2BI CACGAG 1 cut(s) 3948
Bst6I CTCTTC 4 cut(s) 583, 1182, 1631, 3835
BstACI GRCGYC 1 cut(s) 38
BstAFI CTTAAG 2 cut(s) 2218, 2879
BstAUI TGTACA 3 cut(s) 1221, 1575, 3691
BstDSI CCRYGG 1 cut(s) 2946
BstENI CCTNNNNNAGG 3 cut(s) 1151, 2651, 3871
BstH2I RGCGCY 4 cut(s) 112, 436, 1195, 2460
BstHHI GCGC 5 cut(s) 111, 435, 1194, 2459, 3412
BstMAI GTCTC 7 cut(s) 196, 1034, 1934, 2805, 3282, 3837, 3912
BstMWI GCNNNNNNNGC 8 cut(s) 260, 269, 1368, 2151, 2963, 3428, 3641, 4012
BstNSI RCATGY 6 cut(s) 328, 550, 849, 1919, 2957, 3192
BstSFI CTRYAG 3 cut(s) 1422, 1485, 1943
BstSLI GKGCMC 2 cut(s) 614, 1870
BstV1I GCAGC 8 cut(s) 85, 892, 1436, 2166, 2275, 2532, 2731, 3897
BstV2I GAAGAC 1 cut(s) 3082
BstX2I RGATCY 4 cut(s) 228, 1670, 1979, 2815
BstYI RGATCY 4 cut(s) 228, 1670, 1979, 2815
BstZ17I GTATAC 1 cut(s) 3857
Bsu15I ATCGAT 1 cut(s) 3499
Bsu36I CCTNAGG 1 cut(s) 3417
BsuI GTATCC 2 cut(s) 712, 3567
BsuRI GGCC 7 cut(s) 398, 1715, 1743, 1845, 3108, 3286, 4121
BsuTUI ATCGAT 1 cut(s) 3499
BtgI CCRYGG 1 cut(s) 2946
BtgZI GCGATG 2 cut(s) 501, 3520
BtsIMutI CAGTG 9 cut(s) 614, 951, 1231, 1289, 1789, 2078, 2316, 3279, 3470
BveI ACCTGC 1 cut(s) 3640
CciI TCATGA 2 cut(s) 826, 1795
CfoI GCGC 5 cut(s) 111, 435, 1194, 2459, 3412
Cfr13I GGNCC 3 cut(s) 1843, 2838, 3284
ClaI ATCGAT 1 cut(s) 3499
CseI GACGC 1 cut(s) 2855
DraI TTTAAA 3 cut(s) 429, 2853, 4081
DraIII CACNNNGTG 1 cut(s) 1433
DriI GACNNNNNGTC 1 cut(s) 2797
Eam1104I CTCTTC 4 cut(s) 583, 1182, 1631, 3835
Eam1105I GACNNNNNGTC 1 cut(s) 2797
EarI CTCTTC 4 cut(s) 583, 1182, 1631, 3835
Eco130I CCWWGG 5 cut(s) 1276, 1373, 2615, 2783, 2946
Eco147I AGGCCT 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 1723
Eco31I GGTCTC 2 cut(s) 1934, 2805
Eco32I GATATC 2 cut(s) 507, 2029
Eco47I GGWCC 1 cut(s) 2838
Eco47III AGCGCT 2 cut(s) 434, 1193
Eco57I CTGAAG 2 cut(s) 2613, 2633
Eco81I CCTNAGG 1 cut(s) 3417
Eco88I CYCGRG 1 cut(s) 3363
EcoNI CCTNNNNNAGG 3 cut(s) 1151, 2651, 3871
EcoRV GATATC 2 cut(s) 507, 2029
EcoT14I CCWWGG 5 cut(s) 1276, 1373, 2615, 2783, 2946
EcoT22I ATGCAT 2 cut(s) 847, 3242
EcoT38I GRGCYC 1 cut(s) 1723
ErhI CCWWGG 5 cut(s) 1276, 1373, 2615, 2783, 2946
Esp3I CGTCTC 1 cut(s) 196
FaqI GGGAC 3 cut(s) 1583, 2824, 3720
FauI CCCGC 2 cut(s) 247, 621
FbaI TGATCA 2 cut(s) 1138, 2860
FblI GTMKAC 2 cut(s) 2536, 3856
FriOI GRGCYC 1 cut(s) 1723
GlaI GCGC 5 cut(s) 110, 434, 1193, 2458, 3411
GsuI CTGGAG 4 cut(s) 192, 381, 1260, 1508
HaeII RGCGCY 4 cut(s) 112, 436, 1195, 2460
HaeIII GGCC 7 cut(s) 398, 1715, 1743, 1845, 3108, 3286, 4121
HapII CCGG 7 cut(s) 341, 404, 512, 551, 869, 2370, 3781
HgaI GACGC 1 cut(s) 2855
HhaI GCGC 5 cut(s) 111, 435, 1194, 2459, 3412
Hin1I GRCGYC 1 cut(s) 38
Hin6I GCGC 5 cut(s) 109, 433, 1192, 2457, 3410
HinP1I GCGC 5 cut(s) 109, 433, 1192, 2457, 3410
HincII GTYRAC 4 cut(s) 388, 1621, 2668, 2770
HindII GTYRAC 4 cut(s) 388, 1621, 2668, 2770
HindIII AAGCTT 3 cut(s) 833, 3385, 4013
HpaI GTTAAC 1 cut(s) 1621
HpaII CCGG 7 cut(s) 341, 404, 512, 551, 869, 2370, 3781
Hpy166II GTNNAC 9 cut(s) 336, 388, 1561, 1621, 2537, 2668, 2738, 2770, 3857
Hpy8I GTNNAC 9 cut(s) 336, 388, 1561, 1621, 2537, 2668, 2738, 2770, 3857
Hpy99I CGWCG 5 cut(s) 40, 43, 2010, 2871, 3676
HpyAV CCTTC 9 cut(s) 819, 1468, 1665, 2427, 2637, 2657, 3137, 3278, 3877
HpyCH4IV ACGT 2 cut(s) 38, 2008
HpyF10VI GCNNNNNNNGC 8 cut(s) 260, 269, 1368, 2151, 2963, 3428, 3641, 4012
HpySE526I ACGT 2 cut(s) 38, 2008
Hsp92I GRCGYC 1 cut(s) 38
HspAI GCGC 5 cut(s) 109, 433, 1192, 2457, 3410
Kpn2I TCCGGA 1 cut(s) 2369
KpnI GGTACC 1 cut(s) 1909
Ksp22I TGATCA 2 cut(s) 1138, 2860
KspAI GTTAAC 1 cut(s) 1621
LguI GCTCTTC 1 cut(s) 1182
Lsp1109I GCAGC 8 cut(s) 85, 892, 1436, 2166, 2275, 2532, 2731, 3897
MaeII ACGT 2 cut(s) 38, 2008
MfeI CAATTG 1 cut(s) 305
MflI RGATCY 4 cut(s) 228, 1670, 1979, 2815
MhlI GDGCHC 4 cut(s) 614, 1524, 1723, 1870
MlyI GAGTC 3 cut(s) 519, 569, 799
MmeI TCCRAC 5 cut(s) 54, 78, 118, 1878, 3955
Mph1103I ATGCAT 2 cut(s) 847, 3242
MroI TCCGGA 1 cut(s) 2369
MroXI GAANNNNTTC 1 cut(s) 3099
MslI CAYNNNNRTG 5 cut(s) 1326, 1682, 2106, 2494, 2948
MspA1I CMGCKG 2 cut(s) 2157, 3017
MspCI CTTAAG 2 cut(s) 2218, 2879
MspI CCGG 7 cut(s) 341, 404, 512, 551, 869, 2370, 3781
MunI CAATTG 1 cut(s) 305
Mva1269I GAATGC 2 cut(s) 1414, 2925
MwoI GCNNNNNNNGC 8 cut(s) 260, 269, 1368, 2151, 2963, 3428, 3641, 4012
NciI CCSGG 1 cut(s) 870
NcoI CCATGG 1 cut(s) 2946
NheI GCTAGC 1 cut(s) 1358
NlaIV GGNNCC 6 cut(s) 6, 222, 1722, 1907, 2817, 2840
NmuCI GTSAC 4 cut(s) 1298, 2289, 2501, 2683
NsiI ATGCAT 2 cut(s) 847, 3242
NspI RCATGY 6 cut(s) 328, 550, 849, 1919, 2957, 3192
OliI CACNNNNGTG 1 cut(s) 2948
PaeI GCATGC 2 cut(s) 550, 2957
PaeR7I CTCGAG 1 cut(s) 3363
PagI TCATGA 2 cut(s) 826, 1795
PasI CCCWGGG 1 cut(s) 1639
PceI AGGCCT 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 1182
PcsI WCGNNNNNNNCGW 1 cut(s) 2401
PctI GAATGC 2 cut(s) 1414, 2925
PdmI GAANNNNTTC 1 cut(s) 3099
PflFI GACNNNGTC 3 cut(s) 40, 1284, 3167
PflMI CCANNNNNTGG 2 cut(s) 748, 1730
PfoI TCCNGGA 2 cut(s) 1237, 1386
PleI GAGTC 3 cut(s) 519, 569, 798
PpsI GAGTC 3 cut(s) 519, 569, 798
PshBI ATTAAT 2 cut(s) 3615, 3933
PsiI TTATAA 3 cut(s) 1160, 1313, 3587
PspN4I GGNNCC 6 cut(s) 6, 222, 1722, 1907, 2817, 2840
PspPI GGNCC 3 cut(s) 1843, 2838, 3284
PsrI GAACNNNNNNTAC 4 cut(s) 204, 236, 1336, 1368
PstI CTGCAG 2 cut(s) 1426, 1947
PsuI RGATCY 4 cut(s) 228, 1670, 1979, 2815
PsyI GACNNNGTC 3 cut(s) 40, 1284, 3167
RseI CAYNNNNRTG 5 cut(s) 1326, 1682, 2106, 2494, 2948
SapI GCTCTTC 1 cut(s) 1182
Sau96I GGNCC 3 cut(s) 1843, 2838, 3284
ScaI AGTACT 1 cut(s) 2055
SchI GAGTC 3 cut(s) 519, 569, 799
SduI GDGCHC 4 cut(s) 614, 1524, 1723, 1870
SfcI CTRYAG 3 cut(s) 1422, 1485, 1943
Sfr274I CTCGAG 1 cut(s) 3363
SinI GGWCC 1 cut(s) 2838
SlaI CTCGAG 1 cut(s) 3363
SmiI ATTTAAAT 1 cut(s) 2853
SmiMI CAYNNNNRTG 5 cut(s) 1326, 1682, 2106, 2494, 2948
SmlI CTYRAG 6 cut(s) 1535, 2033, 2218, 2879, 3363, 3539
SmoI CTYRAG 6 cut(s) 1535, 2033, 2218, 2879, 3363, 3539
SpeI ACTAGT 2 cut(s) 3709, 3859
SphI GCATGC 2 cut(s) 550, 2957
SseBI AGGCCT 1 cut(s) 398
SspI AATATT 2 cut(s) 1443, 3159
StuI AGGCCT 1 cut(s) 398
StyI CCWWGG 5 cut(s) 1276, 1373, 2615, 2783, 2946
SwaI ATTTAAAT 1 cut(s) 2853
TaiI ACGT 2 cut(s) 41, 2011
TatI WGTACW 8 cut(s) 211, 333, 1221, 1575, 2053, 2114, 2735, 3691
TauI GCSGC 6 cut(s) 73, 149, 185, 359, 2100, 3798
TscAI CASTG 9 cut(s) 614, 951, 1231, 1289, 1789, 2078, 2323, 3279, 3477
TseFI GTSAC 4 cut(s) 1298, 2289, 2501, 2683
TseI GCWGC 8 cut(s) 73, 880, 1424, 2154, 2263, 2520, 2744, 3910
Tsp45I GTSAC 4 cut(s) 1298, 2289, 2501, 2683
TspGWI ACGGA 4 cut(s) 495, 685, 3371, 3689
TspRI CASTG 9 cut(s) 614, 951, 1231, 1289, 1789, 2078, 2323, 3279, 3477
Tth111I GACNNNGTC 3 cut(s) 40, 1284, 3167
Van91I CCANNNNNTGG 2 cut(s) 748, 1730
Vha464I CTTAAG 2 cut(s) 2218, 2879
VpaK11BI GGWCC 1 cut(s) 2838
VspI ATTAAT 2 cut(s) 3615, 3933
XagI CCTNNNNNAGG 3 cut(s) 1151, 2651, 3871
XapI RAATTY 7 cut(s) 288, 346, 1265, 1633, 2280, 2848, 3099
XceI RCATGY 6 cut(s) 328, 550, 849, 1919, 2957, 3192
XcmI CCANNNNNNNNNTGG 1 cut(s) 3961
XhoI CTCGAG 1 cut(s) 3363
XmaJI CCTAGG 2 cut(s) 1373, 2783
XmiI GTMKAC 2 cut(s) 2536, 3856
XmnI GAANNNNTTC 1 cut(s) 3099
ZraI GACGTC 1 cut(s) 39
ZrmI AGTACT 1 cut(s) 2055
Zsp2I ATGCAT 2 cut(s) 847, 3242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.