Rh1BG149800

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002244
Physical Location & Seq
Reverse (-)
24418683 .. 24431017
12335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG149800.1

Sequence Viewer

Length: 4098 bp
ATGGCTCCTAGAAAGAAACCCAATTCTTCTTCTTCTTCTACCACTTCGTCTCGGAAATCGAACTCGAAAGCTCAGCCTCAGCCTTCCAAGTTCAGCATCCAACATTTCTTTGAGCGCCACACCCAGAACTCGCAAAACCCCCAAACCACCACCTCTTCCACTCCTCACAACCCTAAGCCCCCAATCCCCGACTCCGCCACCGCCATTGTTCCGCAGAAGTCGATTCCGGAGATCCAAAACCCTGCCCCGATTGAAGCAAATAGTGCACTAGTAGCTTCTGGAAATCCCGAGAATCGGCGTGATTCGCAGTCGAGTAGTAAGAACAGTACGCAGGAGACACCAACTGAGAATTTGGTGGCAGTGAGGTTTATGGATGCCGAGCCGAATGTGGATGAGGAGGTCTCACCGGAGGTTTCGAAGTCTAAGTCTGTCAAGCGATTCAAGTTCTCCCCTGGAATGTTGATAAAGCAGAGTCAAGATGATGGAGGTGATGAGGTGACGTGGAGGACGTCTCCGGTTAATGAAAGACTTAAAGCAGTTTCAAAGCACAATCCTGAGATGATGAGAGTGTTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACAAATGCTCGCAAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGATAAATCCCTGTCGTTGGTGTCCCTGAATAGGGTTGGCGTGAAGAGGGTTAAACCGGATCAGGATGGGGAACATAATGGAAATGTGGTCGATAGGACTATTTCTGAAATTGCTGGTAGTCAGAGTCCATTCCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTGCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCTGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATTTTTGTTGAAGATTCAGTACCTGATGAAGTGGAGGCATCTCAATTACAAGTTAAAGATAAATCAGAACATGTCGTAAAAAGAGTGGTGACAGCTCCAACAGAACAGGTTACTCGGGCATCTTCCAATTACAGTTTCCTTGTATTGGAGGTATCTGAGAATTGTAGACCTGATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTACGGGAAGAGTGGTTTTACAGTGTCATTGCACCTGGAGATACTGTCAACGTTATTGGTCAATTTGATGACCAAGGACAGTGTCATGTGGATCATCACCATAACTTTTTAATTGTTCACCCCGATGTCTTAGTGTCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCCTAGGCGAACTTTGCTGGATGAAAGGCTAAAAGGCAATGAGTATTCAACTCCAGCACTAATTGGCACAATATTGCATCAAGTTTTTGAGGCTGCACTTATGAAGGAGAAACCCACAATTACTTTCTTGGAGGAACATGCAAGAATGGGGCTCCAGAGAAACATTGAGAATTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTGAACGTCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAGGTCACAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTCGAAGCTAACAAAAATGAATCTGACGTGAAGGTTATGCCTTTGGAGTTTAAAACCGGGAAAGTGCCGAAGGACCAGGAGCATTGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAGAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACGGAGTCCAAGCTTGTGCAGAAGTTGTCGTCATCTGAATGTGTGCACCATCTATCATAAGGCACATGGAGGCAACACAGATAGCAGCGGATTGGGAGATTTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGACTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGCTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGTGATCATTCCACAAGTTGCCTTTCTTCTATGGTTCTTGACAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTGCTGGTTTCTCTTCTCTCAATGTGAGGACATCCAATGGAGATTTTCAGAATGCTGCTTCTTCACCCTCAAAATATGCAGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTAGCTGTTGCAAGGGGGGTCATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGGGCAATCCTTCTACAGAGGCAAGTGATCTTCATCGGGAGGTCTGGCGGGTTGACAAAGATGAATTTATGACATCATTTGCCACAATGAGGTTCAACCTTCTTCAACTTTTTCTTCCGGGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGGTTTGACAGTGGATGTATTACATTCAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTCGTGCCATTCTAAAGATACTTACAGCGAAGGACTACGCTCTTATACTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGCGAGGTGCATCAATTTTGCTTACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTGCGCATTGGAAGACAGGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCTTTTCAGCAATGAACATACAAAGTGTGGAAGACATTAAGTCAAGATTGGACCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAACAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTCGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCCCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCACATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGTATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGACAGATTGCGTTGTGGATCTCCTGAAATAGCCAATGCCAAACTCAACTTTTCAAGTTCGAAGTCTCATTCACTGTGGATAAAGGAGGTTTTAAATCCAAGCAAACCAGTTATATTTATTAATACAGATGCTTTACCTGCTTTCGAGGCAAAGGACCACAAGATTGTGAATAACCCGATGGAAGCTCACATAGTTGTAGAGGTCGTGGAGGAATTAGTTAACAGTGGAATTGAAGGGGATGATATTGGCATCATTACACCGTATAACTCCCAGGCAGAGATCATTCGGCTTGCCATCAACCTTACTTCCGTGGAGATACATACCATTGACAAATACCAGGGAAGAGATAAAGATTGCATACTAGTCTCCTTTGTTAGGTCAACCGAAAATCCACGAAACTGCACGTCCTCTCTGCTCGGAGACTGGCATAGGATTAATGTGGCTTTAACTCGTGCCAAGAAAAAGTTGATAATGGTCGGGTCACGCGAAACCCTATCCAAAGTTCTGCTGATGAAGCTTCTTATCGAGAAAGTTGATGAACAATCAGGCATCTTCAACGTGTCCAAGAAAGACATTAACTTTAAGAGGGAGCTCAAGAGATGCTCTCAGATAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

1365

Amino Acids

151.7

Weight (kDa)

7.11

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 382 - 584 5.8e-63 DNA replication factor Dna2
AAA_11 PF13086 945 - 1036 1.6e-16 AAA domain
AAA_30 PF13604 946 - 1109 2.7e-09 AAA domain
AAA_19 PF13245 950 - 1109 5.2e-07 AAA domain
AAA_11 PF13086 1045 - 1113 5.9e-16 AAA domain
AAA_12 PF13087 1122 - 1322 8e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
Acc16I TGCGCA 1 cut(s) 3053
Acc36I ACCTGC 1 cut(s) 3625
Acc65I GGTACC 1 cut(s) 1881
AccB1I GGYRCC 2 cut(s) 1881, 2717
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccI GTMKAC 2 cut(s) 1087, 1545
AccII CGCG 1 cut(s) 3966
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 7 cut(s) 195, 201, 212, 1946, 2133, 2263, 2644
AclI AACGTT 2 cut(s) 1224, 1553
AclWI GGATC 8 cut(s) 226, 720, 1272, 1648, 2795, 2808, 3191, 3505
AcoI YGGCCR 1 cut(s) 2264
AcsI RAATTY 7 cut(s) 349, 1510, 2360, 2660, 2833, 2995, 3212
AcuI CTGAAG 1 cut(s) 1638
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 5 cut(s) 328, 942, 1883, 3340, 3431
AflIII ACRYGT 2 cut(s) 991, 4038
AhdI GACNNNNNGTC 2 cut(s) 2027, 3136
AhlI ACTAGT 3 cut(s) 268, 3188, 3841
AjiI CACGTC 3 cut(s) 501, 1783, 3885
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
Alw21I GWGCWC 4 cut(s) 268, 1111, 2093, 4074
Alw26I GTCTC 7 cut(s) 54, 329, 406, 516, 3549, 3850, 3894
Alw44I GTGCAC 2 cut(s) 264, 2089
AlwI GGATC 8 cut(s) 226, 720, 1272, 1648, 2795, 2808, 3191, 3505
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 4 cut(s) 2013, 2264, 3269, 3345
ApaLI GTGCAC 2 cut(s) 264, 2089
ApeKI GCWGC 5 cut(s) 874, 1325, 1433, 2130, 2435
ApoI RAATTY 7 cut(s) 349, 1510, 2360, 2660, 2833, 2995, 3212
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AseI ATTAAT 2 cut(s) 3600, 3915
Asp700I GAANNNNTTC 2 cut(s) 1595, 3084
Asp718I GGTACC 1 cut(s) 1881
AspA2I CCTAGG 2 cut(s) 1343, 2765
AspLEI GCGC 2 cut(s) 117, 3054
AspS9I GGNCC 6 cut(s) 1828, 2823, 3148, 3269, 3346, 3634
AsuC2I CCSGG 2 cut(s) 1813, 2715
AsuII TTCGAA 2 cut(s) 416, 3539
AvaI CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
AvaII GGWCC 4 cut(s) 1828, 2823, 3148, 3634
AvrII CCTAGG 2 cut(s) 1343, 2765
AxyI CCTNAGG 2 cut(s) 2195, 3402
BaeGI GKGCMC 4 cut(s) 268, 1846, 2093, 2722
BamHI GGATCC 1 cut(s) 2800
BanI GGYRCC 2 cut(s) 1881, 2717
BanII GRGCYC 3 cut(s) 1494, 1708, 4074
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 1 cut(s) 3930
BbsI GAAGAC 4 cut(s) 1588, 1898, 3067, 3135
Bbv12I GWGCWC 4 cut(s) 268, 1111, 2093, 4074
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 5 cut(s) 861, 1337, 1420, 2142, 2422
BccI CCATC 8 cut(s) 476, 713, 839, 1690, 2102, 2230, 3652, 3782
BcgI CGANNNNNNTGC 2 cut(s) 3078, 3112
BclI TGATCA 4 cut(s) 1927, 2284, 2845, 3307
BcnI CCSGG 2 cut(s) 1813, 2715
BcoDI GTCTC 7 cut(s) 54, 329, 406, 516, 3549, 3850, 3894
BcuI ACTAGT 3 cut(s) 268, 3188, 3841
BfaI CTAG 9 cut(s) 9, 269, 1344, 2516, 2756, 2766, 2900, 3189, 3842
BfmI CTRYAG 1 cut(s) 2610
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 3625
BglII AGATCT 1 cut(s) 1955
BisI GCNGC 6 cut(s) 875, 1326, 1434, 2131, 2264, 2436
BlnI CCTAGG 2 cut(s) 1343, 2765
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 6 cut(s) 876, 1327, 1435, 2132, 2265, 2437
Bme18I GGWCC 4 cut(s) 1828, 2823, 3148, 3634
BmeRI GACNNNNNGTC 2 cut(s) 2027, 3136
BmeT110I CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
BmgBI CACGTC 3 cut(s) 501, 1783, 3885
BmgT120I GGNCC 6 cut(s) 1828, 2823, 3148, 3269, 3346, 3634
BmiI GGNNCC 8 cut(s) 6, 1493, 1707, 1883, 2719, 2763, 2802, 2825
BpiI GAAGAC 4 cut(s) 1588, 1898, 3067, 3135
BplI GAGNNNNNCTC 5 cut(s) 386, 418, 496, 528, 4069
BpmI CTGGAG 4 cut(s) 1230, 1377, 1478, 1904
Bpu10I CCTNAGC 2 cut(s) 78, 174
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 2 cut(s) 416, 3539
BpuEI CTTGAG 2 cut(s) 2002, 4058
BpuMI CCSGG 2 cut(s) 1813, 2715
BsaBI GATNNNNATC 2 cut(s) 2628, 3312
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 406
BsaWI WCCGGW 4 cut(s) 226, 406, 514, 709
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bse1I ACTGG 7 cut(s) 1906, 1921, 2213, 3257, 3427, 3587, 3908
Bse21I CCTNAGG 2 cut(s) 2195, 3402
Bse3DI GCAATG 6 cut(s) 887, 1200, 1384, 3094, 3114, 3416
Bse8I GATNNNNATC 2 cut(s) 2628, 3312
BseAI TCCGGA 1 cut(s) 226
BseJI GATNNNNATC 2 cut(s) 2628, 3312
BseMI GCAATG 6 cut(s) 887, 1200, 1384, 3094, 3114, 3416
BseNI ACTGG 7 cut(s) 1906, 1921, 2213, 3257, 3427, 3587, 3908
BseRI GAGGAG 4 cut(s) 153, 410, 1706, 3315
BseSI GKGCMC 4 cut(s) 268, 1846, 2093, 2722
BseXI GCAGC 5 cut(s) 861, 1337, 1420, 2142, 2422
BsgI GTGCAG 3 cut(s) 1419, 2083, 3865
Bsh1236I CGCG 1 cut(s) 3966
BshFI GGCC 4 cut(s) 2015, 2266, 3271, 3347
BshNI GGYRCC 2 cut(s) 1881, 2717
BsiHKAI GWGCWC 4 cut(s) 268, 1111, 2093, 4074
BsiHKCI CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
BsiSI CCGG 6 cut(s) 227, 407, 515, 710, 1812, 2714
BslFI GGGAC 5 cut(s) 661, 1553, 2219, 2809, 3177
BsmAI GTCTC 7 cut(s) 54, 329, 406, 516, 3549, 3850, 3894
BsmBI CGTCTC 2 cut(s) 54, 516
BsmFI GGGAC 5 cut(s) 661, 1553, 2219, 2809, 3177
BsmI GAATGC 2 cut(s) 2437, 2910
BsnI GGCC 4 cut(s) 2015, 2266, 3271, 3347
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
Bsp119I TTCGAA 2 cut(s) 416, 3539
Bsp1286I GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1846, 2093, 2722, 4074
Bsp13I TCCGGA 1 cut(s) 226
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 7 cut(s) 195, 201, 212, 1946, 2133, 2263, 2644
BspANI GGCC 4 cut(s) 2015, 2266, 3271, 3347
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 3966
BspHI TCATGA 2 cut(s) 814, 1531
BspLI GGNNCC 8 cut(s) 6, 1493, 1707, 1883, 2719, 2763, 2802, 2825
BspMI ACCTGC 1 cut(s) 3625
BspPI GGATC 8 cut(s) 226, 720, 1272, 1648, 2795, 2808, 3191, 3505
BspT104I TTCGAA 2 cut(s) 416, 3539
BspT107I GGYRCC 2 cut(s) 1881, 2717
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 6 cut(s) 887, 1200, 1384, 3094, 3114, 3416
BsrI ACTGG 7 cut(s) 1906, 1921, 2213, 3257, 3427, 3587, 3908
BssNAI GTATAC 1 cut(s) 1546
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 3930
BssT1I CCWWGG 3 cut(s) 1246, 1343, 2765
Bst1107I GTATAC 1 cut(s) 1546
Bst2BI CACGAG 1 cut(s) 3930
Bst6I CTCTTC 9 cut(s) 160, 692, 1152, 1176, 1869, 2273, 2398, 3329, 3817
BstACI GRCGYC 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBI TTCGAA 2 cut(s) 416, 3539
BstC8I GCNNGC 7 cut(s) 617, 825, 2382, 2940, 3201, 3383, 3771
BstDSI CCRYGG 1 cut(s) 3789
BstEII GGTNACC 1 cut(s) 2480
BstENI CCTNNNNNAGG 2 cut(s) 2913, 3853
BstFNI CGCG 1 cut(s) 3966
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 3054
BstMAI GTCTC 7 cut(s) 54, 329, 406, 516, 3549, 3850, 3894
BstNSI RCATGY 7 cut(s) 995, 1120, 1481, 1524, 1895, 2942, 3177
BstPI GGTNACC 1 cut(s) 2480
BstSFI CTRYAG 1 cut(s) 2610
BstSLI GKGCMC 4 cut(s) 268, 1846, 2093, 2722
BstUI CGCG 1 cut(s) 3966
BstV1I GCAGC 5 cut(s) 861, 1337, 1420, 2142, 2422
BstV2I GAAGAC 4 cut(s) 1588, 1898, 3067, 3135
BstX2I RGATCY 5 cut(s) 231, 1640, 1955, 2800, 3497
BstYI RGATCY 5 cut(s) 231, 1640, 1955, 2800, 3497
BstZ17I GTATAC 1 cut(s) 1546
Bsu36I CCTNAGG 2 cut(s) 2195, 3402
BsuRI GGCC 4 cut(s) 2015, 2266, 3271, 3347
BtgI CCRYGG 1 cut(s) 3789
BtrI CACGTC 3 cut(s) 501, 1783, 3885
BtsI GCAGTG 2 cut(s) 366, 2506
BtsIMutI CAGTG 8 cut(s) 366, 1118, 1201, 1259, 2506, 2782, 3551, 3709
BveI ACCTGC 1 cut(s) 3625
Cac8I GCNNGC 7 cut(s) 617, 825, 2382, 2940, 3201, 3383, 3771
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 2 cut(s) 814, 1531
CfoI GCGC 2 cut(s) 117, 3054
Cfr13I GGNCC 6 cut(s) 1828, 2823, 3148, 3269, 3346, 3634
CseI GACGC 1 cut(s) 2840
Csp6I GTAC 5 cut(s) 327, 941, 1882, 3339, 3430
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 2256, 2291
CviQI GTAC 5 cut(s) 327, 941, 1882, 3339, 3430
DraI TTTAAA 3 cut(s) 1807, 2838, 3573
DriI GACNNNNNGTC 2 cut(s) 2027, 3136
EaeI YGGCCR 1 cut(s) 2264
Eam1104I CTCTTC 9 cut(s) 160, 692, 1152, 1176, 1869, 2273, 2398, 3329, 3817
Eam1105I GACNNNNNGTC 2 cut(s) 2027, 3136
EarI CTCTTC 9 cut(s) 160, 692, 1152, 1176, 1869, 2273, 2398, 3329, 3817
EciI GGCGGA 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 4072
Eco130I CCWWGG 3 cut(s) 1246, 1343, 2765
Eco147I AGGCCT 1 cut(s) 2015
Eco24I GRGCYC 3 cut(s) 1494, 1708, 4074
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 4 cut(s) 1828, 2823, 3148, 3634
Eco53kI GAGCTC 1 cut(s) 4072
Eco57I CTGAAG 1 cut(s) 1638
Eco81I CCTNAGG 2 cut(s) 2195, 3402
Eco88I CYCGRG 5 cut(s) 287, 1035, 1317, 3266, 3348
Eco91I GGTNACC 1 cut(s) 2480
EcoICRI GAGCTC 1 cut(s) 4072
EcoNI CCTNNNNNAGG 2 cut(s) 2913, 3853
EcoO65I GGTNACC 1 cut(s) 2480
EcoT14I CCWWGG 3 cut(s) 1246, 1343, 2765
EcoT22I ATGCAT 2 cut(s) 1522, 3227
EcoT38I GRGCYC 3 cut(s) 1494, 1708, 4074
ErhI CCWWGG 3 cut(s) 1246, 1343, 2765
Esp3I CGTCTC 2 cut(s) 54, 516
FaqI GGGAC 5 cut(s) 661, 1553, 2219, 2809, 3177
FauI CCCGC 1 cut(s) 2637
FbaI TGATCA 4 cut(s) 1927, 2284, 2845, 3307
FblI GTMKAC 2 cut(s) 1087, 1545
Fnu4HI GCNGC 6 cut(s) 875, 1326, 1434, 2131, 2264, 2436
FriOI GRGCYC 3 cut(s) 1494, 1708, 4074
Fsp4HI GCNGC 6 cut(s) 875, 1326, 1434, 2131, 2264, 2436
FspAI RTGCGCAY 1 cut(s) 3053
FspBI CTAG 9 cut(s) 9, 269, 1344, 2516, 2756, 2766, 2900, 3189, 3842
FspI TGCGCA 1 cut(s) 3053
GlaI GCGC 2 cut(s) 116, 3053
GluI GCNGC 6 cut(s) 875, 1326, 1434, 2131, 2264, 2436
GsuI CTGGAG 4 cut(s) 1230, 1377, 1478, 1904
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 4 cut(s) 2015, 2266, 3271, 3347
HapII CCGG 6 cut(s) 227, 407, 515, 710, 1812, 2714
HgaI GACGC 1 cut(s) 2840
HhaI GCGC 2 cut(s) 117, 3054
Hin1I GRCGYC 1 cut(s) 509
Hin6I GCGC 2 cut(s) 115, 3052
HinP1I GCGC 2 cut(s) 115, 3052
HincII GTYRAC 5 cut(s) 1222, 2650, 2752, 3700, 3861
HindII GTYRAC 5 cut(s) 1222, 2650, 2752, 3700, 3861
HindIII AAGCTT 5 cut(s) 821, 1620, 2056, 3370, 3995
HpaI GTTAAC 1 cut(s) 3700
HpaII CCGG 6 cut(s) 227, 407, 515, 710, 1812, 2714
Hpy99I CGWCG 1 cut(s) 2856
HpyCH4IV ACGT 9 cut(s) 500, 509, 1224, 1553, 1593, 1782, 1984, 3884, 4038
HpySE526I ACGT 9 cut(s) 500, 509, 1224, 1553, 1593, 1782, 1984, 3884, 4038
Hsp92I GRCGYC 1 cut(s) 509
HspAI GCGC 2 cut(s) 115, 3052
Kpn2I TCCGGA 1 cut(s) 226
KpnI GGTACC 1 cut(s) 1885
Ksp22I TGATCA 4 cut(s) 1927, 2284, 2845, 3307
KspAI GTTAAC 1 cut(s) 3700
LmnI GCTCC 8 cut(s) 10, 1021, 1497, 1711, 1834, 2239, 2767, 4069
Lsp1109I GCAGC 5 cut(s) 861, 1337, 1420, 2142, 2422
MaeI CTAG 9 cut(s) 9, 269, 1344, 2516, 2756, 2766, 2900, 3189, 3842
MaeII ACGT 9 cut(s) 500, 509, 1224, 1553, 1593, 1782, 1984, 3884, 4038
MflI RGATCY 5 cut(s) 231, 1640, 1955, 2800, 3497
MhlI GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1846, 2093, 2722, 4074
MlyI GAGTC 4 cut(s) 185, 481, 787, 2059
MmeI TCCRAC 3 cut(s) 124, 1043, 1854
Mph1103I ATGCAT 2 cut(s) 1522, 3227
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 2 cut(s) 1595, 3084
MslI CAYNNNNRTG 5 cut(s) 1296, 1536, 2082, 2473, 2556
MspA1I CMGCKG 1 cut(s) 2133
MspI CCGG 6 cut(s) 227, 407, 515, 710, 1812, 2714
Mva1269I GAATGC 2 cut(s) 2437, 2910
MvnI CGCG 1 cut(s) 3966
NciI CCSGG 2 cut(s) 1813, 2715
NlaIV GGNNCC 8 cut(s) 6, 1493, 1707, 1883, 2719, 2763, 2802, 2825
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 7 cut(s) 496, 1009, 1663, 2480, 2549, 3479, 3960
NsbI TGCGCA 1 cut(s) 3053
NsiI ATGCAT 2 cut(s) 1522, 3227
NspI RCATGY 7 cut(s) 995, 1120, 1481, 1524, 1895, 2942, 3177
NspV TTCGAA 2 cut(s) 416, 3539
PaeI GCATGC 1 cut(s) 2942
PagI TCATGA 2 cut(s) 814, 1531
PceI AGGCCT 1 cut(s) 2015
PciI ACATGT 1 cut(s) 991
PcsI WCGNNNNNNNCGW 2 cut(s) 506, 3963
PctI GAATGC 2 cut(s) 2437, 2910
PdmI GAANNNNTTC 2 cut(s) 1595, 3084
PflFI GACNNNGTC 2 cut(s) 1254, 3152
PflMI CCANNNNNTGG 1 cut(s) 1715
PkrI GCNGC 6 cut(s) 876, 1327, 1435, 2132, 2265, 2437
PleI GAGTC 4 cut(s) 185, 480, 786, 2058
PpsI GAGTC 4 cut(s) 185, 480, 786, 2058
PscI ACATGT 1 cut(s) 991
PshBI ATTAAT 2 cut(s) 3600, 3915
Psp124BI GAGCTC 1 cut(s) 4074
Psp1406I AACGTT 2 cut(s) 1224, 1553
PspEI GGTNACC 1 cut(s) 2480
PspN4I GGNNCC 8 cut(s) 6, 1493, 1707, 1883, 2719, 2763, 2802, 2825
PspPI GGNCC 6 cut(s) 1828, 2823, 3148, 3269, 3346, 3634
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 5 cut(s) 231, 1640, 1955, 2800, 3497
PsyI GACNNNGTC 2 cut(s) 1254, 3152
RsaI GTAC 5 cut(s) 328, 942, 1883, 3340, 3431
RsaNI GTAC 5 cut(s) 327, 941, 1882, 3339, 3430
RseI CAYNNNNRTG 5 cut(s) 1296, 1536, 2082, 2473, 2556
SacI GAGCTC 1 cut(s) 4074
SatI GCNGC 6 cut(s) 875, 1326, 1434, 2131, 2264, 2436
Sau96I GGNCC 6 cut(s) 1828, 2823, 3148, 3269, 3346, 3634
SchI GAGTC 4 cut(s) 185, 481, 787, 2059
SduI GDGCHC 8 cut(s) 268, 1111, 1494, 1708, 1846, 2093, 2722, 4074
SfcI CTRYAG 1 cut(s) 2610
SfuI TTCGAA 2 cut(s) 416, 3539
SinI GGWCC 4 cut(s) 1828, 2823, 3148, 3634
SmiI ATTTAAAT 1 cut(s) 2838
SmiMI CAYNNNNRTG 5 cut(s) 1296, 1536, 2082, 2473, 2556
SmlI CTYRAG 2 cut(s) 2017, 4073
SmoI CTYRAG 2 cut(s) 2017, 4073
SpeI ACTAGT 3 cut(s) 268, 3188, 3841
SphI GCATGC 1 cut(s) 2942
SseBI AGGCCT 1 cut(s) 2015
SsiI CCGC 7 cut(s) 195, 201, 212, 1946, 2133, 2263, 2644
SspI AATATT 3 cut(s) 1413, 1578, 3291
SspMI CTAG 9 cut(s) 9, 269, 1344, 2516, 2756, 2766, 2900, 3189, 3842
SstI GAGCTC 1 cut(s) 4074
StuI AGGCCT 1 cut(s) 2015
StyI CCWWGG 3 cut(s) 1246, 1343, 2765
SwaI ATTTAAAT 1 cut(s) 2838
TaiI ACGT 9 cut(s) 503, 512, 1227, 1556, 1596, 1785, 1987, 3887, 4041
TatI WGTACW 1 cut(s) 3338
TauI GCSGC 1 cut(s) 2266
TscAI CASTG 8 cut(s) 366, 1118, 1201, 1259, 2506, 2782, 3558, 3709
TseFI GTSAC 7 cut(s) 496, 1009, 1663, 2480, 2549, 3479, 3960
TseI GCWGC 5 cut(s) 874, 1325, 1433, 2130, 2435
Tsp45I GTSAC 7 cut(s) 496, 1009, 1663, 2480, 2549, 3479, 3960
TspGWI ACGGA 2 cut(s) 2062, 3778
TspRI CASTG 8 cut(s) 366, 1118, 1201, 1259, 2506, 2782, 3558, 3709
Tth111I GACNNNGTC 2 cut(s) 1254, 3152
Van91I CCANNNNNTGG 1 cut(s) 1715
VneI GTGCAC 2 cut(s) 264, 2089
VpaK11BI GGWCC 4 cut(s) 1828, 2823, 3148, 3634
VspI ATTAAT 2 cut(s) 3600, 3915
XagI CCTNNNNNAGG 2 cut(s) 2913, 3853
XapI RAATTY 7 cut(s) 349, 1510, 2360, 2660, 2833, 2995, 3212
XceI RCATGY 7 cut(s) 995, 1120, 1481, 1524, 1895, 2942, 3177
XmaJI CCTAGG 2 cut(s) 1343, 2765
XmiI GTMKAC 2 cut(s) 1087, 1545
XmnI GAANNNNTTC 2 cut(s) 1595, 3084
XspI CTAG 9 cut(s) 9, 269, 1344, 2516, 2756, 2766, 2900, 3189, 3842
ZraI GACGTC 1 cut(s) 510
Zsp2I ATGCAT 2 cut(s) 1522, 3227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.