Rh2BG309800

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002244
Physical Location & Seq
Forward (+)
36191736 .. 36193018
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG309800.1

Sequence Viewer

Length: 474 bp
ATGCAAAGAATGGGGATCCAGAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCACAAAAATATTAAATTGGGTTAACATCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAAGTCGCAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTTGAAGCTAACAAAAATGAACCTGACGTGAAGGTTATGCCTTCGGAGTTTAAAACCGGGAAAGTGCCAAAGGACCAGGAGCATAGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACTTGATCTTTATTCCTCATTCGTTTTTTTTTTTCTTTCCCTTTAGTACGTATAACGGTATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

157

Amino Acids

17.89

Weight (kDa)

5.63

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 6 - 92 3e-21 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 1 cut(s) 69
AclI AACGTT 1 cut(s) 77
AclWI GGATC 3 cut(s) 10, 23, 172
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 407, 454
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 124, 284
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 2 cut(s) 132, 354
AluBI AGCT 2 cut(s) 146, 287
AluI AGCT 2 cut(s) 146, 287
AlwI GGATC 3 cut(s) 10, 23, 172
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 190, 286
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 370
BamHI GGATCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 232
BccI CCATC 1 cut(s) 214
BciT130I CCWGG 2 cut(s) 134, 356
BcnI CCSGG 1 cut(s) 337
Bme1390I CCNGG 3 cut(s) 134, 337, 356
Bme18I GGWCC 1 cut(s) 352
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 17, 231
BmrFI CCNGG 3 cut(s) 134, 337, 356
BmsI GCATC 2 cut(s) 76, 250
BpuMI CCSGG 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 456
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 239
BseBI CCWGG 2 cut(s) 134, 356
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 2 cut(s) 159, 189
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 232, 370
Bsp143I GATC 3 cut(s) 15, 164, 411
BspCNI CTCAG 2 cut(s) 160, 190
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 17, 231
BspPI GGATC 3 cut(s) 10, 23, 172
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 15, 164, 411
BssNAI GTATAC 1 cut(s) 70
Bst1107I GTATAC 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 134, 356
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 168, 198
BstKTI GATC 3 cut(s) 18, 167, 414
BstMBI GATC 3 cut(s) 15, 164, 411
BstNI CCWGG 2 cut(s) 134, 356
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 3 cut(s) 132, 335, 354
BstSLI GKGCMC 1 cut(s) 370
BstSNI TACGTA 1 cut(s) 456
BstX2I RGATCY 2 cut(s) 15, 164
BstYI RGATCY 2 cut(s) 15, 164
BstZ17I GTATAC 1 cut(s) 70
BtrI CACGTC 1 cut(s) 307
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 406, 453
CviAII CATG 3 cut(s) 45, 56, 225
CviJI RGCY 3 cut(s) 146, 230, 287
CviKI_1 RGCY 3 cut(s) 146, 230, 287
CviQI GTAC 2 cut(s) 406, 453
DdeI CTNAG 2 cut(s) 168, 198
DpnI GATC 3 cut(s) 17, 166, 413
DpnII GATC 3 cut(s) 15, 164, 411
DraI TTTAAA 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco105I TACGTA 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 132, 354
EcoT22I ATGCAT 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 232
FaeI CATG 3 cut(s) 48, 59, 228
FatI CATG 3 cut(s) 44, 55, 224
FblI GTMKAC 1 cut(s) 69
FriOI GRGCYC 1 cut(s) 232
HapII CCGG 1 cut(s) 336
Hin1II CATG 3 cut(s) 48, 59, 228
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 1 cut(s) 137
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 1 cut(s) 336
HphI GGTGA 2 cut(s) 190, 286
Hpy166II GTNNAC 2 cut(s) 70, 115
Hpy188I TCNGA 5 cut(s) 142, 169, 199, 325, 400
Hpy188III TCNNGA 2 cut(s) 19, 56
Hpy8I GTNNAC 2 cut(s) 70, 115
HpyAV CCTTC 2 cut(s) 304, 330
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4IV ACGT 3 cut(s) 77, 306, 455
HpyCH4V TGCA 2 cut(s) 4, 44
HpyF3I CTNAG 2 cut(s) 168, 198
HpySE526I ACGT 3 cut(s) 77, 306, 455
Hsp92II CATG 3 cut(s) 48, 59, 228
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 15, 164, 411
LmnI GCTCC 2 cut(s) 235, 358
LpnPI CCDG 8 cut(s) 32, 119, 146, 256, 315, 341, 349, 368
LweI GCATC 2 cut(s) 76, 250
MaeII ACGT 3 cut(s) 77, 306, 455
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 17, 166, 413
MboI GATC 3 cut(s) 15, 164, 411
MboII GAAGA 2 cut(s) 112, 380
MflI RGATCY 2 cut(s) 15, 164
MhlI GDGCHC 2 cut(s) 232, 370
MluCI AATT 2 cut(s) 106, 204
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 4 cut(s) 193, 208, 396, 432
Mph1103I ATGCAT 1 cut(s) 46
MseI TTAA 3 cut(s) 104, 114, 330
MslI CAYNNNNRTG 1 cut(s) 60
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 3 cut(s) 134, 337, 356
MvaI CCWGG 2 cut(s) 134, 356
NciI CCSGG 1 cut(s) 337
NdeII GATC 3 cut(s) 15, 164, 411
NlaIII CATG 3 cut(s) 48, 59, 228
NlaIV GGNNCC 2 cut(s) 17, 231
NmuCI GTSAC 1 cut(s) 91
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 1 cut(s) 48
PagI TCATGA 1 cut(s) 55
PfeI GAWTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 456
Psp1406I AACGTT 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 132, 354
PspGI CCWGG 2 cut(s) 132, 354
PspN4I GGNNCC 2 cut(s) 17, 231
PspPI GGNCC 1 cut(s) 352
PsuI RGATCY 2 cut(s) 15, 164
RsaI GTAC 2 cut(s) 407, 454
RsaNI GTAC 2 cut(s) 406, 453
RseI CAYNNNNRTG 1 cut(s) 60
SaqAI TTAA 3 cut(s) 104, 114, 330
Sau3AI GATC 3 cut(s) 15, 164, 411
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 3 cut(s) 134, 337, 356
SduI GDGCHC 2 cut(s) 232, 370
SfaNI GCATC 2 cut(s) 76, 250
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 60
SnaBI TACGTA 1 cut(s) 456
Sse9I AATT 2 cut(s) 106, 204
SspI AATATT 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 132, 335, 354
TaaI ACNGT 1 cut(s) 464
TaiI ACGT 3 cut(s) 80, 309, 458
TasI AATT 2 cut(s) 106, 204
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 3 cut(s) 104, 114, 330
Tru9I TTAA 3 cut(s) 104, 114, 330
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 4 cut(s) 44, 72, 138, 312
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 352
XceI RCATGY 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.