RchiOBHm_Chr2g0122951

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002244
Physical Location & Seq
Forward (+)
36088073 .. 36089131
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49529

Sequence Viewer

Length: 423 bp
ATGAGTCAAGACGATGGAGGTGATGAGGTGACATGGAGGACGTCTCCGGTCAATGAAAGACTTAAAGCAGTTTCAAAGCACAAGCCTGAGATGATGAGAGTATTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGAAAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGAGAAATCCCTGTCGTTGGTGTCCCTGAATAGGGTTGGCGTGAAAAGGTTTAAACCAGATCAGGATGTAGAACAAAATGGAAATGTGGTCGATAGGACTATTTCTGAAATGGCTGGTAGTCAGAGTCCATTCCGGACTCCACATTCTTTATCATACTGTCATGATAAGCTTCCTAATGCTCTTTCATGTGGTGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATATAAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

140

Amino Acids

15.36

Weight (kDa)

9.07

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 44
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 202, 216, 217
AgsI TTSAA 3 cut(s) 75, 117, 173
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 2 cut(s) 176, 355
AluI AGCT 2 cut(s) 176, 355
Alw26I GTCTC 1 cut(s) 48
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 32, 40
BccI CCATC 1 cut(s) 8
BciT130I CCWGG 1 cut(s) 396
BcoDI GTCTC 1 cut(s) 48
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
Bme1390I CCNGG 1 cut(s) 396
BmrFI CCNGG 1 cut(s) 396
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 395
BsaWI WCCGGW 2 cut(s) 46, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 216, 217
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 3 cut(s) 202, 216, 217
BseMII CTCAG 1 cut(s) 78
BshFI GGCC 1 cut(s) 406
BsiSI CCGG 2 cut(s) 47, 319
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 3 cut(s) 202, 216, 217
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 1 cut(s) 406
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 1 cut(s) 407
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 1 cut(s) 79
BspEI TCCGGA 1 cut(s) 318
BspHI TCATGA 1 cut(s) 346
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 344
BstACI GRCGYC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 394
BsuRI GGCC 1 cut(s) 406
BtsCI GGATG 1 cut(s) 256
CciI TCATGA 1 cut(s) 346
CviAII CATG 3 cut(s) 33, 347, 372
CviJI RGCY 7 cut(s) 85, 121, 176, 299, 355, 394, 406
CviKI_1 RGCY 7 cut(s) 85, 121, 176, 299, 355, 394, 406
DdeI CTNAG 1 cut(s) 87
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DraI TTTAAA 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 394
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 3 cut(s) 36, 350, 375
FaiI YATR 7 cut(s) 34, 340, 348, 373, 411, 413, 421
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 32, 346, 371
Fnu4HI GCNGC 1 cut(s) 407
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 407
GluI GCNGC 1 cut(s) 407
HaeIII GGCC 1 cut(s) 406
HapII CCGG 2 cut(s) 47, 319
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 3 cut(s) 36, 350, 375
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 3 cut(s) 4, 310, 322
HpaII CCGG 2 cut(s) 47, 319
HphI GGTGA 2 cut(s) 32, 40
Hpy188I TCNGA 3 cut(s) 292, 309, 388
Hpy188III TCNNGA 4 cut(s) 8, 248, 319, 347
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 3 cut(s) 36, 350, 375
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 1 cut(s) 244
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MlyI GAGTC 3 cut(s) 13, 316, 319
MnlI CCTC 4 cut(s) 11, 19, 30, 396
MroI TCCGGA 1 cut(s) 318
MseI TTAA 4 cut(s) 63, 131, 138, 237
MspI CCGG 2 cut(s) 47, 319
MspR9I CCNGG 1 cut(s) 396
MssI GTTTAAAC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 1 cut(s) 244
NlaIII CATG 3 cut(s) 36, 350, 375
NmuCI GTSAC 1 cut(s) 28
PagI TCATGA 1 cut(s) 346
PkrI GCNGC 1 cut(s) 408
PleI GAGTC 3 cut(s) 12, 316, 318
PmeI GTTTAAAC 1 cut(s) 238
PpsI GAGTC 3 cut(s) 12, 316, 318
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
SaqAI TTAA 4 cut(s) 63, 131, 138, 237
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 1 cut(s) 244
SchI GAGTC 3 cut(s) 13, 316, 319
ScrFI CCNGG 1 cut(s) 396
SetI ASST 8 cut(s) 22, 30, 44, 178, 186, 236, 357, 420
SsiI CCGC 1 cut(s) 407
StyD4I CCNGG 1 cut(s) 394
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 149, 276
TauI GCSGC 1 cut(s) 409
Tru1I TTAA 4 cut(s) 63, 131, 138, 237
Tru9I TTAA 4 cut(s) 63, 131, 138, 237
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 69, 360
ZraI GACGTC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.